CN104076141A - Positioning calibrator for enzyme label plate washing all-in-one machine - Google Patents
Positioning calibrator for enzyme label plate washing all-in-one machine Download PDFInfo
- Publication number
- CN104076141A CN104076141A CN201410361863.1A CN201410361863A CN104076141A CN 104076141 A CN104076141 A CN 104076141A CN 201410361863 A CN201410361863 A CN 201410361863A CN 104076141 A CN104076141 A CN 104076141A
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- Prior art keywords
- calibration
- lower panel
- enzyme
- plate
- washing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
The invention provides a positioning calibrator for an enzyme label plate washing all-in-one machine. The positioning calibrator for the enzyme label plate washing all-in-one machine is characterized by comprising an upper panel (4) and a lower panel (5), wherein the dimension of the lower panel (5) is matched with that of the enzyme label plate washing all-in-one machine; the dimensions of two sides or four sides to which the upper panel (4) is opposite are greater than that of the lower panel (5); an array of penetrating plate washing calibration holes (2) which penetrate through the upper panel (4) and the lower panel (5) are used for calibrating the waterway are formed in the upper panel (4); enzyme label colorimetric calibration positions (1) comprising crossed vertical stripes (7) and horizontal stripes (6) are arranged in areas except for the areas in which the plate washing calibration holes (2) are formed in the upper panel (4). The positioning calibrator for the enzyme label plate washing all-in-one machine is simple in structure, convenient to manufacture, is capable of remarkably improving the check precision and speed and ensures the normal work of the instrument.
Description
Technical field
The present invention relates to a kind of medicine equipment, especially a kind of medical science Biochemical Analyzer carries out the calibrating installation of periodic calibration, specifically location calibrating device for a kind of Enzyme-linked washing-board all-in-one.
Background technology
At present, Enzyme-linked washing-board all-in-one is a kind of common seralyzer, in order to guarantee testing accuracy, need to regularly carry out verification, and upper on the market in order to calibration, what wash plate machine is mainly to calibrate water route by ELISA Plate, because enzyme mark boring ratio is washed large many of the water absorber of plate machine, tend to occur deviation, the naked eyes that rely on judge more, and naked eyes are also not easy observation, result is to wash more than plate machine washing washs residual quantity 5 μ L, affects colorimetric result, occur that result is inaccurate, also bad judgement washes that plate machine goes wrong or the result that microplate reader place causes is inaccurate; The detection of light path simultaneously often adopts directly blank ELISA Plate is carried out to reading, so just exist the otherness rate of exchange of reading large, such as repetition measurement variation lines, repeatedly the coefficient of variation of reading result is up to more than 10%, and this just has a strong impact on the accuracy of testing result, must be overcome.
Summary of the invention
The object of the invention is for existing Enzyme-linked washing-board all-in-one because lacking the desirable instruments of inspection, but utilize ELISA Plate to carry out water route calibration, light path calibration is directly joined target by blank and is caused detection error large as reference foundation, precision is low, and mainly rely on the problem of artificial experience judgement, design a kind of location calibrating device for Enzyme-linked washing-board all-in-one that can accurately simultaneously carry out light path and water route check.
Technical scheme of the present invention is:
Location calibrating device for a kind of Enzyme-linked washing-board all-in-one, it is characterized in that it is comprised of top panel 4 and lower panel 5, the size of lower panel 5 and Enzyme-linked washing-board all-in-one match, the relative both sides of top panel 4 or the size on four limits are greater than the size of lower panel 5, in the above plate 4 be provided with a row run through top panel 4 and lower panel 5 run through for what calibrate water route, wash plate calibration hole 2, in the above on plate 4 except wash all the other regions plate calibration hole 2 regions be provided with by the intersection of nicking 7 and horizontal stripe 6 form for calibrating the enzyme mark colorimetric calibration bits 1 of light path.
The described plate calibration hole 2 of washing is several rows of row, and quantity is 8.
The orientation of described enzyme mark colorimetric calibration bits 1 is with to wash plate calibration hole 2 consistent, and every row has 8 enzyme mark colorimetric calibration bits 1.
Described top panel 4 and lower panel 5 relative position places are processed with pilot hole 3.
Beneficial effect of the present invention:
The plate calibration hole of washing of the present invention is than the large 2mm of the water absorber of washing plate machine, more much smaller than blank ELISA Plate, and by the wash residual amount after this device calibration lower than 2 μ L, repetition measurement variation lines is less than or equal to ± 2%.
Calibrating device of the present invention is as the accessory of automatic enzyme immune analytic system, for regularly (every 6 months) calibration of client provides correcting device accurately and reliably, it can guarantee to wash the accurate of Board position, light path position, the position that inspection light beam all concentrates on 8 road photoelectric sensors and washes plate machine note, imbibition is in the center of microwell plate, thus the accuracy and precision of assurance washing, reading.
The present invention is simple in structure, manufactures easy to usely, can significantly improve precision and the speed of verification, guarantees that instrument normally works.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
X-direction calibration process schematic diagram when Fig. 2 is water route of the present invention calibration.
Y-direction calibration process schematic diagram when Fig. 3 is water route of the present invention calibration.
Fig. 4 is that calibrating device of the present invention carries out the light path schematic diagram in when calibration.
Fig. 5 is washing state reading schematic diagram of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.
As shown in Figure 1.
Location calibrating device for a kind of Enzyme-linked washing-board all-in-one, it is comprised of top panel 4 and lower panel 5, the size of lower panel 5 and Enzyme-linked washing-board all-in-one match, the relative both sides of top panel 4 or the size on four limits are greater than the size of lower panel 5, in the above plate 4 be provided with a row totally 8 run through top panel 4 and lower panel 5 run through for what calibrate water route, wash plate calibration hole 2, in the above on plate 4 except wash all the other regions plate calibration hole 2 regions be provided with by the intersection of nicking 7 and horizontal stripe 6 form for calibrating the enzyme mark colorimetric calibration bits 1 of light path, the orientation of every row's enzyme mark colorimetric calibration bits 1 is with to wash 2 orientations of plate calibration hole consistent, quantity is also 8 of every rows, for easy to process, plate 4 and lower panel 5 relative position places are also processed with processing pilot hole 3 in the above.
During concrete enforcement, top panel 4 and lower panel 5 also can be designed to an one-piece construction, adopt injection mo(u)lding or machining moulding, then by design size processing, wash plate calibration hole 2, portray nicking 7 and horizontal stripe 6 and form enzyme mark colorimetric calibration bits 1.As shown in Figure 1.
Using method of the present invention is:
Enzyme-linked washing-board all-in-one of the present invention location calibrating device is placed on to automatic enzyme immune analytic system and hatches zone position place, by debugging software, control the position that Enzyme-linked washing-board all-in-one runs to appointment, such as washing plate calibration bits 2 in Fig. 1, carry out respectively in X-direction, calibrating as shown in Figure 2, in Y direction, calibrate as shown in Figure 3, after calibration, action command AD(washes the decline of plate machine), washing plate chance drops to and washes plate calibration hole 2, AU(washes plate machine and rises) mention and wash plate machine, if malposition, can block or be offset touching and wash plate calibration hole 2.By debugging software, control the position that Enzyme-linked washing-board all-in-one enzyme mark light source position runs to appointment equally as shown in Figure 4, as the enzyme mark colorimetric calibration bits 1 in figure, then move LA(lamp bright), LS(lamp goes out), whether observation light source luminous point is just in time in enzyme mark colorimetric calibration bits 1, have or not skew, can be by fine setting optocoupler position correction if having; Finally action command AD, AU, LA, LS observation X, Y direction are washed location of reading as shown in Figure 5, detect the accurate of washing, location of reading; General every 6 Ge Yue producers can be by long-range or visit as customer service.
The part that the present invention does not relate to all prior art that maybe can adopt same as the prior art is realized.
Claims (4)
1. an Enzyme-linked washing-board all-in-one is with locating calibrating device, it is characterized in that it is comprised of top panel (4) and lower panel (5), size and the Enzyme-linked washing-board all-in-one of lower panel (5) match, the relative both sides of top panel (4) or the size on four limits are greater than the size of lower panel (5), in the above plate (4) be provided with a row run through top panel (4) and lower panel (5) run through for what calibrate water route, wash plate calibration hole (2), in the above plate (4) upper except wash all the other regions plate calibration hole (2) region be provided with by the intersection of nicking (7) and horizontal stripe (6) form for calibrating the enzyme mark colorimetric calibration bits (1) of light path.
2. calibrating device according to claim 1, is characterized in that the described plate calibration hole (2) of washing is several rows of row, and quantity is 8.
3. calibrating device according to claim 1, the orientation that it is characterized in that described enzyme mark colorimetric calibration bits (1) is with to wash plate calibration hole (2) consistent, and every row has 8 enzyme mark colorimetric calibration bits (1).
4. calibrating device according to claim 1, is characterized in that described top panel (4) and lower panel (5) relative position place are processed with pilot hole (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361863.1A CN104076141B (en) | 2014-07-28 | 2014-07-28 | Enzyme-linked washing-board all-in-one location calibrating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361863.1A CN104076141B (en) | 2014-07-28 | 2014-07-28 | Enzyme-linked washing-board all-in-one location calibrating device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104076141A true CN104076141A (en) | 2014-10-01 |
| CN104076141B CN104076141B (en) | 2015-09-16 |
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| CN201410361863.1A Active CN104076141B (en) | 2014-07-28 | 2014-07-28 | Enzyme-linked washing-board all-in-one location calibrating device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999015888A1 (en) * | 1997-09-19 | 1999-04-01 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| EP1369699A1 (en) * | 1999-10-15 | 2003-12-10 | PE Corporation (NY) | System and method for filling a substrate with a liquid sample |
| CN2695954Y (en) * | 2004-04-27 | 2005-04-27 | 中国兵器工业集团第五三研究所 | Standard plate investigating / correcting for enzymic standard analyzer |
| US20100200405A1 (en) * | 2009-02-09 | 2010-08-12 | Thomas Lenz | Devices, systems and methods for separating magnetic particles |
| CN204116352U (en) * | 2014-07-28 | 2015-01-21 | 江苏雷奥生物科技有限公司 | Enzyme-linked washing-board all-in-one location calibrating device |
-
2014
- 2014-07-28 CN CN201410361863.1A patent/CN104076141B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999015888A1 (en) * | 1997-09-19 | 1999-04-01 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| EP1369699A1 (en) * | 1999-10-15 | 2003-12-10 | PE Corporation (NY) | System and method for filling a substrate with a liquid sample |
| CN2695954Y (en) * | 2004-04-27 | 2005-04-27 | 中国兵器工业集团第五三研究所 | Standard plate investigating / correcting for enzymic standard analyzer |
| US20100200405A1 (en) * | 2009-02-09 | 2010-08-12 | Thomas Lenz | Devices, systems and methods for separating magnetic particles |
| CN204116352U (en) * | 2014-07-28 | 2015-01-21 | 江苏雷奥生物科技有限公司 | Enzyme-linked washing-board all-in-one location calibrating device |
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| Publication number | Publication date |
|---|---|
| CN104076141B (en) | 2015-09-16 |
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