CN104076141B - Enzyme-linked washing-board all-in-one location calibrating device - Google Patents
Enzyme-linked washing-board all-in-one location calibrating device Download PDFInfo
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- CN104076141B CN104076141B CN201410361863.1A CN201410361863A CN104076141B CN 104076141 B CN104076141 B CN 104076141B CN 201410361863 A CN201410361863 A CN 201410361863A CN 104076141 B CN104076141 B CN 104076141B
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- enzyme
- top panel
- washing
- board
- lower panel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
A kind of Enzyme-linked washing-board all-in-one location calibrating device, it is characterized in that it is made up of top panel (4) and lower panel (5), size and the Enzyme-linked washing-board all-in-one of lower panel (5) match, the relative both sides of top panel (4) or the size on four limits are greater than the size of lower panel (5), top panel (4) is provided with a row run through top panel (4) and lower panel (5) run through wash plate calibration hole (2) for what calibrate water route, all the other regions on top panel (4) except washing plate calibration hole (2) region are provided with enzyme mark colorimetric calibration bits (1) for calibrating light path formed by the intersection of nicking (7) and horizontal stripe (6).Structure of the present invention is simple, easily manufactured, can significantly improve precision and the speed of verification, guarantee that instrument normally works.
Description
Technical field
The present invention relates to a kind of medicine equipment, especially a kind of medical science Biochemical Analyzer carries out the calibrating installation of periodic calibration, specifically a kind of Enzyme-linked washing-board all-in-one location calibrating device.
Background technology
At present, Enzyme-linked washing-board all-in-one is a kind of common seralyzer, in order to ensure testing accuracy, need regularly to verify, and upper wash trigger in order to calibration on the market mainly carry out calibration water route by ELISA Plate, the water absorber washing trigger due to enzyme mark boring ratio is large many, often there is deviation, many dependence naked eyes judge, and naked eyes are also not easy observation, result washes trigger wash residual amount 5 more than μ L, affects Colorimetric results, occur that result is forbidden, also bad judgement washes that trigger goes wrong or the result that microplate reader place causes is inaccurate; The detection of light path simultaneously often adopts and directly carries out reading to blank ELISA Plate, the otherness rate of exchange that so just there is reading are large, such as the coefficient of variation of repetition measurement variation lines repeatedly reading result is up to more than 10%, and this just has a strong impact on the accuracy of testing result, must be overcome.
Summary of the invention
The object of the invention is for existing Enzyme-linked washing-board all-in-one because lacking the desirable instruments of inspection, but utilize ELISA Plate to carry out water route calibration, light path calibration is directly joined target by blank and is caused metrical error large as with reference to foundation, precision is low, and the main problem relying on artificial experience to judge, design a kind of Enzyme-linked washing-board all-in-one location calibrating device that accurately simultaneously can carry out light path and water route inspection.
Technical scheme of the present invention is:
A kind of Enzyme-linked washing-board all-in-one location calibrating device, it is characterized in that it is made up of top panel 4 and lower panel 5, size and the Enzyme-linked washing-board all-in-one of lower panel 5 match, the relative both sides of top panel 4 or the size on four limits are greater than the size of lower panel 5, top panel 4 is provided with a row run through top panel 4 and lower panel 5 run through wash plate calibration hole 2 for what calibrate water route, all the other regions on top panel 4 except washing plate calibration hole 2 region are provided with the enzyme mark colorimetric calibration bits 1 for calibrating light path formed by the intersection of nicking 7 and horizontal stripe 6.
Described washes plate calibration hole 2 in several rows of row, and quantity is 8.
The orientation of described enzyme mark colorimetric calibration bits 1 is consistent with washing plate calibration hole 2, and often row has 8 enzyme mark colorimetric calibration bits 1.
Described top panel 4 and lower panel 5 relative position are processed with pilot hole 3.
Beneficial effect of the present invention:
Of the present inventionly wash plate calibration hole 2mm larger than the water absorber washing trigger, more much smaller than blank ELISA Plate, and by the wash residual amount after the calibration of this device lower than 2 μ L, repetition measurement variation lines is less than or equal to ± and 2%.
Calibrating device of the present invention is as the accessory of Automatic enzyme-linked immunosorbent assay systems, for client's regularly (every 6 months) calibration provides correcting device accurately and reliably, it can guarantee to wash Board position, light path position accurate, check light beam all concentrate on 8 road photoelectric sensors and wash trigger note, imbibition position in the center of microwell plate, thus ensure washing, reading accuracy and precision.
Structure of the present invention is simple, manufactures easy to use, can significantly improve precision and the speed of verification, guarantee that instrument normally works.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
X when Fig. 2 is water route of the present invention calibration is to calibration process schematic diagram.
Y-direction calibration process schematic diagram when Fig. 3 is water route of the present invention calibration.
Fig. 4 is the schematic diagram of calibrating device of the present invention when carrying out light path calibration.
Fig. 5 is washing state reading schematic diagram of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.
As shown in Figure 1.
A kind of Enzyme-linked washing-board all-in-one location calibrating device, it is made up of top panel 4 and lower panel 5, size and the Enzyme-linked washing-board all-in-one of lower panel 5 match, the relative both sides of top panel 4 or the size on four limits are greater than the size of lower panel 5, top panel 4 is provided with a row totally 8 run through top panel 4 and lower panel 5 run through wash plate calibration hole 2 for what calibrate water route, all the other regions on top panel 4 except washing plate calibration hole 2 region are provided with the enzyme mark colorimetric calibration bits 1 for calibrating light path formed by the intersection of nicking 7 and horizontal stripe 6, the orientation of often arranging enzyme mark colorimetric calibration bits 1 is consistent with washing plate calibration hole 2 orientation, quantity is also often arrange 8, in order to easy to process, locating and machining hole 3 is also processed with at top panel 4 and lower panel 5 relative position.
During concrete enforcement, top panel 4 and lower panel 5 also can be designed to an one-piece construction, adopt injection mo(u)lding or machining shaping, then wash plate calibration hole 2 by design size processing, portray nicking 7 and horizontal stripe 6 forms enzyme mark colorimetric calibration bits 1.As shown in Figure 1.
Using method of the present invention is:
Enzyme-linked washing-board all-in-one location of the present invention calibrating device is placed on Automatic enzyme-linked immunosorbent assay systems and hatches zone position place, control Enzyme-linked washing-board all-in-one by debugging software and run to the position of specifying, plate calibration bits 2 is washed in such as Fig. 1, carry out respectively calibrating in X-direction as shown in Figure 2, calibrate in Y direction as shown in Figure 3, after calibration, action command AD(washes trigger decline), wash trigger can drop to and wash plate calibration hole 2, AU(washes trigger and rises) mention and wash trigger, if malposition, can block or offset touching and wash plate calibration hole 2.As shown in Figure 4 same by debugging software control Enzyme-linked washing-board all-in-one enzyme mark light source position run to the position of specifying, as the enzyme mark colorimetric calibration bits 1 in figure, then run LA(lamp bright), LS(lamp goes out), whether observation light source luminous point is just in time in enzyme mark colorimetric calibration bits 1, with or without skew, if having by fine setting optocoupler position correction; Last action command AD as shown in Figure 5, AU, LA, LS observe X, Y direction washing location of reading, detect washing, location of reading accurate; Generally every 6 Ge Yue producers can by long-range or visit as customer service.
The part that the present invention does not relate to prior art that maybe can adopt all same as the prior art is realized.
Claims (3)
1. an Enzyme-linked washing-board all-in-one location calibrating device, it is characterized in that it is made up of top panel (4) and lower panel (5), size and the Enzyme-linked washing-board all-in-one of lower panel (5) match, the relative both sides of top panel (4) or the size on four limits are greater than the size of lower panel (5), what top panel (4) was provided with a row runs through top panel (4) and lower panel (5) washes plate calibration hole (2) for what calibrate water route, all the other regions on top panel (4) except washing plate calibration hole (2) region are provided with and intersect by nicking (7) and horizontal stripe (6) enzyme mark colorimetric calibration bits (1) for calibrating light path formed, described top panel (4) and lower panel (5) relative position are processed with pilot hole (3).
2. calibrating device according to claim 1, it is characterized in that described washing plate calibration hole (2) in several rows of row, quantity is 8.
3. calibrating device according to claim 1, is characterized in that the orientation of described enzyme mark colorimetric calibration bits (1) is consistent with washing plate calibration hole (2), and often row has 8 enzyme mark colorimetrics calibration bits (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361863.1A CN104076141B (en) | 2014-07-28 | 2014-07-28 | Enzyme-linked washing-board all-in-one location calibrating device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361863.1A CN104076141B (en) | 2014-07-28 | 2014-07-28 | Enzyme-linked washing-board all-in-one location calibrating device |
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| Publication Number | Publication Date |
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| CN104076141A CN104076141A (en) | 2014-10-01 |
| CN104076141B true CN104076141B (en) | 2015-09-16 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN204116352U (en) * | 2014-07-28 | 2015-01-21 | 江苏雷奥生物科技有限公司 | Enzyme-linked washing-board all-in-one location calibrating device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999015888A1 (en) * | 1997-09-19 | 1999-04-01 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| EP1369699A1 (en) * | 1999-10-15 | 2003-12-10 | PE Corporation (NY) | System and method for filling a substrate with a liquid sample |
| CN2695954Y (en) * | 2004-04-27 | 2005-04-27 | 中国兵器工业集团第五三研究所 | Standard plate investigating / correcting for enzymic standard analyzer |
| EP2394175B1 (en) * | 2009-02-09 | 2016-02-03 | caprotec bioanalytics GmbH | Devices, systems and methods for separating magnetic particles |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN204116352U (en) * | 2014-07-28 | 2015-01-21 | 江苏雷奥生物科技有限公司 | Enzyme-linked washing-board all-in-one location calibrating device |
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