CN104073356A - Technology for extracting asarum volatile oil by adopting complex enzyme-supercritical fluid method - Google Patents
Technology for extracting asarum volatile oil by adopting complex enzyme-supercritical fluid method Download PDFInfo
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- CN104073356A CN104073356A CN201410266487.8A CN201410266487A CN104073356A CN 104073356 A CN104073356 A CN 104073356A CN 201410266487 A CN201410266487 A CN 201410266487A CN 104073356 A CN104073356 A CN 104073356A
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Abstract
The invention relates to a technology for extracting asarum volatile oil by adopting a complex enzyme-supercritical fluid method. The technology for extracting the asarum volatile oil by adopting the complex enzyme-supercritical fluid method comprises the following processing steps: fermenting a complex enzyme raw material, and carrying out supercritical CO2 extraction on the fermented raw material, so that the asarum volatile oil is obtained. The technology for extracting the asarum volatile oil by adopting the complex enzyme-supercritical fluid method is simple in operation, fast in extraction speed and high in yield, and the yield of the volatile oil is high while the extracted volatile oil has good quality, so that the technology for extracting the asarum volatile oil by adopting the complex enzyme-supercritical fluid method is a high-efficiency and energy-saving asarum volatile oil extracting and separating method.
Description
Technical field
The present invention relates to the technique that a kind of prozyme-supercritical fluid method extracts Herba Asari volatile oil, belong to extracted form natural plant product technical field.
Background technology
Herba Asari, belongs to aristolochiaceae plant asarum herbal dry herb for many years, and practise and claim Herba Asari, be China's traditional Chinese medicine material.Have antipyretic, dispel the wind, sweating, analgesic activity, be used for the treatment of the diseases such as flu, cough, asthma, be used for the treatment of the diseases such as rhinitis, laryngitis, gastritis and oral erosion modern age, all have significant curative effect.Herba Asari is containing volatile oil 2.5%, and its main component is methyl eugenol, safrole etc., and methyl eugenol has the multiple physiologically actives such as antianaphylaxis, antihistamine, anesthesia, hypotensive, antidepressant, antibechic, analgesia, and safrole is stronger broad-spectrum antimicrobial composition.
At present, the extraction about Herba Asari volatile oil adopts steam distillation, organic solvent extractionprocess etc. mostly.The extraction temperature of these methods is generally higher, and required time is long, and technical process is complicated, easily cause decomposition, variation and the loss of effective constituent, and organic solvent residual is large.Supercritical fluid extraction is with liquid CO
2for solvent extracts, there is nontoxic, no solvent residue, pollution-free, extraction yield advantages of higher, whole process does not produce " three wastes ", can be to environment.By literature search, have no identical with the present invention open source literature report.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of method of utilizing the collaborative supercritical fluid method of mixed enzyme fermentation to extract Herba Asari volatile oil is provided, have that technique is simple, extraction time is short, yield advantages of higher.
The present invention includes following steps:
A. the root of Chinese wild ginger is pulverized, and crosses 40 ~ 60 mesh sieves;
B. add the prozyme that is equivalent to raw material weight 1 ~ 2%, mix, be put in mash-back, add distilled water agglomerating to holding, sealing, in 40 ~ 50 ℃ of fermentations 3 ~ 5 days;
C. the root of Chinese wild ginger meal after fermentation is joined to supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20 ~ 40MPa is set, 40 ~ 50 ℃ of temperature, pass into 25 ~ 35L/h, extraction 1 ~ 3h, and the separation temperature of separating still is 20 ~ 30 ℃, pressure 10 ~ 15MPa, the material parsing is Herba Asari volatile oil.
Biological enzyme of the present invention is comprised of the enzyme of following weight percent: cellulase 40 ~ 50%, hemicellulase 30 ~ 40%, zytase 10 ~ 30%, the weight percent sum of various enzymes is 100%.
The unit of activity of enzyme of the present invention is 6000U/g.
Beneficial effect of the present invention is: (1) adopts prozyme aging process, can destroy the cell walls of plant, is conducive to the stripping of effective constituent, has effectively improved the extraction yield of Herba Asari volatile oil; (2) the present invention adopts supercritical CO
2extraction mode; the feature with low-temperature extraction and inertia protection; oxidation and the pyrolytic decomposition of " susceptibility " material have been prevented; treasury is got, separation of double acts on one; whole process does not produce " three wastes ", can be to environment, and easy to operate; technique is simple, and production safety is reliable.
Below in conjunction with embodiment, further illustrate the present invention, but the scope of protection of present invention is not limited to following embodiment.
embodiment:
Embodiment 1:
The root of Chinese wild ginger is pulverized, cross 60 mesh sieves, get dried powder 500g, add the prozyme (cellulase, hemicellulase and zytase are by weight the mixed enzyme of 0.5:0.4:0.1, and the unit of activity of enzyme is 6000U/g) that is equivalent to raw material weight 1%, mix, be put in mash-back, adding distil water is agglomerating to holding, sealing, in 50 ℃ of fermentations 3 days, the root of Chinese wild ginger meal after fermentation is joined to supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20MPa is set, temperature 50 C, passes into 35L/h, extraction 1h, and the separation temperature of separating still is 30 ℃, and pressure 12MPa parses Herba Asari volatile oil 16.2g, and yield is 3.24%.
Embodiment 2:
The root of Chinese wild ginger is pulverized, cross 50 mesh sieves, get dried powder 500g, add the prozyme (cellulase, hemicellulase and zytase are by weight the mixed enzyme of 0.45:0.35:0.2, and the unit of activity of enzyme is 6000U/g) that is equivalent to raw material weight 1.5%, mix, be put in mash-back, adding distil water is agglomerating to holding, sealing, in 45 ℃ of fermentations 4 days, the root of Chinese wild ginger meal after fermentation is joined to supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 30MPa is set, 40 ℃ of temperature, pass into 25L/h, extraction 2h, and the separation temperature of separating still is 20 ℃, and pressure 10MPa parses Herba Asari volatile oil 20.55g, and yield is 4.11%.
Embodiment 3:
The root of Chinese wild ginger is pulverized, cross 40 mesh sieves, get dried powder 500g, add the prozyme (cellulase, hemicellulase and zytase are by weight the mixed enzyme of 0.4:0.3:0.3, and the unit of activity of enzyme is 6000U/g) that is equivalent to raw material weight 2%, mix, be put in mash-back, adding distil water is agglomerating to holding, sealing, in 40 ℃ of fermentations 5 days, the root of Chinese wild ginger meal after fermentation is joined to supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 40MPa is set, temperature 45 C, passes into 30L/h, extraction 3h, and the separation temperature of separating still is 25 ℃, and pressure 15MPa parses Herba Asari volatile oil 19.3g, and yield is 3.86%.
Claims (3)
1. prozyme-supercritical fluid method extracts a technique for Herba Asari volatile oil, it is characterized in that, comprises the following steps:
A. the root of Chinese wild ginger is pulverized, and crosses 40 ~ 60 mesh sieves;
B. add the prozyme that is equivalent to raw material weight 1 ~ 2%, mix, be put in mash-back, add distilled water agglomerating to holding, sealing, in 40 ~ 50 ℃ of fermentations 3 ~ 5 days;
C. the root of Chinese wild ginger meal after fermentation is joined to supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20 ~ 40MPa is set, 40 ~ 50 ℃ of temperature, pass into 25 ~ 35L/h, extraction 1 ~ 3h, and the separation temperature of separating still is 20 ~ 30 ℃, pressure 10 ~ 15MPa, the material parsing is Herba Asari volatile oil.
2. a kind of prozyme according to claim 1-supercritical fluid method extracts the technique of Herba Asari volatile oil, it is characterized in that, described biological enzyme is comprised of the enzyme of following weight percent: cellulase 40 ~ 50%, hemicellulase 30 ~ 40%, zytase 10 ~ 30%, the weight percent sum of various enzymes is 100%.
3. a kind of prozyme according to claim 1-supercritical fluid method extracts the technique of Herba Asari volatile oil, it is characterized in that, the unit of activity of described enzyme is 6000U/g.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105542966A (en) * | 2016-01-29 | 2016-05-04 | 常州达奥新材料科技有限公司 | Method for extracting high-yield citrus essential oil |
| WO2016065994A1 (en) * | 2014-10-30 | 2016-05-06 | 广州医科大学附属第一医院 | Method for extracting high-purity asarinin by supercritical carbon dioxide extraction method and use thereof |
| CN108034501A (en) * | 2017-12-30 | 2018-05-15 | 刘莹艳 | A kind of essential oil gel and preparation method thereof |
| CN111704960A (en) * | 2020-06-24 | 2020-09-25 | 江西思派思香料化工有限公司 | Extraction process of high-purity eucalyptus oil |
| CN113273683A (en) * | 2021-05-31 | 2021-08-20 | 四川省雅士科技有限公司 | Method for extracting spice oleoresin under assistance of complex enzyme |
| CN114989887A (en) * | 2022-05-18 | 2022-09-02 | 湖北澳利龙食品股份有限公司 | Complex enzyme assisted supercritical CO 2 Method for extracting houttuynin |
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2014
- 2014-06-16 CN CN201410266487.8A patent/CN104073356A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016065994A1 (en) * | 2014-10-30 | 2016-05-06 | 广州医科大学附属第一医院 | Method for extracting high-purity asarinin by supercritical carbon dioxide extraction method and use thereof |
| CN105542966A (en) * | 2016-01-29 | 2016-05-04 | 常州达奥新材料科技有限公司 | Method for extracting high-yield citrus essential oil |
| CN108034501A (en) * | 2017-12-30 | 2018-05-15 | 刘莹艳 | A kind of essential oil gel and preparation method thereof |
| CN111704960A (en) * | 2020-06-24 | 2020-09-25 | 江西思派思香料化工有限公司 | Extraction process of high-purity eucalyptus oil |
| CN113273683A (en) * | 2021-05-31 | 2021-08-20 | 四川省雅士科技有限公司 | Method for extracting spice oleoresin under assistance of complex enzyme |
| CN114989887A (en) * | 2022-05-18 | 2022-09-02 | 湖北澳利龙食品股份有限公司 | Complex enzyme assisted supercritical CO 2 Method for extracting houttuynin |
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Application publication date: 20141001 |