CN104073359A - Process for extracting alpinia oxyphylla volatile oil through biological enzyme-supercritical fluid method - Google Patents
Process for extracting alpinia oxyphylla volatile oil through biological enzyme-supercritical fluid method Download PDFInfo
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Abstract
The invention relates to a process for extracting alpinia oxyphylla volatile oil through a biological enzyme-supercritical fluid method. The process comprises the following treatment steps: performing raw material compound enzyme fermentation, adding the fermented raw materials into an extraction kettle of a supercritical CO2 fluid extractor, setting the extraction pressure to be 20-30MPa and the temperature to be 30-45 DEG C, controlling introducing rate at 20-30L/h, and extracting for 1.5-3 hours, wherein the temperature of the separation kettle I is 20-30 DEG C, the pressure is 10-20MPa, the temperature of the separation kettle II is 10-20 DEG C, the pressure is 5-15MPa, and the separated substance is the alpinia oxyphylla volatile oil. The process is high in extraction speed, high in yield, convenient to operate, safe, reliable and pollution-free, the obtained product has high yield and purity, and the process is a high-efficiency energy-saving method for extracting and separating the alpinia oxyphylla volatile oil.
Description
Technical field
The present invention relates to a kind of extracting method of Fructus Alpinae Oxyphyllae volatile oil, be specifically related to the technique of a kind of biological enzyme-supercritical fluid method extraction Fructus Alpinae Oxyphyllae volatile oil, belong to extracted form natural plant product technical field.
Background technology
Intelligence development, belong to zingiberaceous plant, solely produce south China, aboundresources, another name Sharpleaf Galangal Fruit, intelligence development, be one of Hainan Si great Nan medicine, there is warm spleen, warm kidney, solid and gas, puckery essence, control cold air stomachache, night the effect such as many urine, its main component is volatile oil, and in oil, main component has eucalyptol, zingiberene, zingiberol etc.Fructus Alpinae Oxyphyllae volatile oil has the aspect effects such as extraordinary maincenter inhibition and analgesia, is a kind of medicinal oil of very worth exploitation.
Enzyme engineering technology, utilize enzyme liquid to divide and be deconstructed into cell walls and cytoplasmic Mierocrystalline cellulose, hemicellulose and pectin substance etc., make it loosen, expand, collapse, thereby increased the diffusion area of effective constituent, reduce resistance to mass transfer, improved by a relatively large margin the extraction yield of active ingredients in natural plants.
Supercritical fluid extraction technology is to utilize supercutical fluid for solvent, extracts some active principle or monomer from solid or liquid, and a kind of technology separating.There is the feature of low-temperature extraction and inertia protection; prevent contingent reacting to each other between the oxidation of " susceptibility " material and loss and different components; make extraction and fractionation be easy to unite two into one; effectively improve production efficiency; can exempt in process of production any poisonous and hazardous organic solvent completely; extract, not containing organic solvent residual, has kept the All Pure Nature of extract.Whole process does not produce " three wastes ", can be to environment.By literature search, have no identical with the present invention open source literature report.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting volatile oil from intelligence development that technique is simple, extraction time is short, yield is high.
The present invention includes following steps:
Intelligence development dried powder sprays the biological enzyme that is equivalent to intelligence development weight 0.5 ~ 1%, and film wrapped, is deposited in the darkroom of 45 ~ 50 DEG C of temperature, fermentation 12 ~ 24h, and the raw material stoving after fermentation, joins supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20 ~ 30MPa is set, 30 ~ 45 DEG C of temperature, pass into 20 ~ 30L/h, extraction 1.5 ~ 3h, 20 ~ 30 DEG C of separating still I temperature, pressure 10 ~ 20MPa, 10 ~ 20 DEG C of separating still II temperature, pressure 5 ~ 15MPa, the material parsing is Fructus Alpinae Oxyphyllae volatile oil.
Biological enzyme of the present invention is made up of the enzyme of following weight percent: Mierocrystalline cellulose 40 ~ 50%, hemicellulose 30 ~ 40%, amylase 10 ~ 30%, the weight percent sum of various enzymes is 100%.
Beneficial effect of the present invention is: (1) adopts prozyme processing, can destroy the cell walls of plant, is conducive to the stripping of effective constituent, greatly shortens the treatment time, enhances productivity; (2) treasury of the present invention is got, separation of double acts on one, and easy to operate, technique is simple, pollution-free, and production safety is reliable.
Further illustrate the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1:
Get intelligence development dried powder 1000g, spray the biological enzyme (cellulase: hemicellulase: amylase is 0.4:0.4:0.2) that is equivalent to intelligence development weight 0.5%, film wrapped, be deposited in the darkroom of temperature 45 C, fermentation 24h, the raw material stoving after fermentation, joins supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20MPa is set, temperature 45 C, passes into 20L/h, extraction 3h, and 30 DEG C of separating still I temperature, pressure 10MPa, 10 DEG C of separating still II temperature, pressure 5MPa, the material parsing is Fructus Alpinae Oxyphyllae volatile oil, and yield is 2.3%.
Embodiment 2:
Get intelligence development dried powder 1000g, spray the biological enzyme (cellulase: hemicellulase: amylase is 0.4:0.3:0.3) that is equivalent to intelligence development weight 0.8%, film wrapped, be deposited in the darkroom of temperature 50 C, fermentation 18h, the raw material stoving after fermentation, joins supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 25MPa is set, 40 DEG C of temperature, pass into 25L/h, extraction 1.5h, 25 DEG C of separating still I temperature, pressure 15MPa, 15 DEG C of separating still II temperature, pressure 10MPa, the material parsing is Fructus Alpinae Oxyphyllae volatile oil, and yield is 2.6%.
Embodiment 3:
Get intelligence development dried powder 1000g, spray the biological enzyme (cellulase: hemicellulase: amylase is 0.5:0.3:0.2) that is equivalent to intelligence development weight 1%, film wrapped, be deposited in the darkroom of temperature 45 C, fermentation 12h, the raw material stoving after fermentation, joins supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 30MPa is set, 35 DEG C of temperature, pass into 30L/h, extraction 2h, and 20 DEG C of separating still I temperature, pressure 20MPa, 20 DEG C of separating still II temperature, pressure 15MPa, the material parsing is Fructus Alpinae Oxyphyllae volatile oil, and yield is 2.5%.
Claims (2)
1. the technique of biological enzyme-supercritical fluid method extraction Fructus Alpinae Oxyphyllae volatile oil, it is characterized in that, comprise the following steps: intelligence development dried powder sprays the biological enzyme that is equivalent to intelligence development weight 0.5 ~ 1%, film wrapped, be deposited in the darkroom of 45 ~ 50 DEG C of temperature, fermentation 12 ~ 24h, the raw material stoving after fermentation, joins supercritical CO
2in the extraction kettle of fluid extraction instrument, extracting pressure 20 ~ 30MPa is set, 30 ~ 45 DEG C of temperature, pass into 20 ~ 30L/h, extraction 1.5 ~ 3h, 20 ~ 30 DEG C of separating still I temperature, pressure 10 ~ 20MPa, 10 ~ 20 DEG C of separating still II temperature, pressure 5 ~ 15MPa, the material parsing is Fructus Alpinae Oxyphyllae volatile oil.
2. a kind of biological enzyme according to claim 1-supercritical fluid method extracts the technique of Fructus Alpinae Oxyphyllae volatile oil, it is characterized in that, described biological enzyme is made up of the enzyme of following weight percent: Mierocrystalline cellulose 40 ~ 50%, hemicellulose 30 ~ 40%, amylase 10 ~ 30%, the weight percent sum of various enzymes is 100%.
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| CN201410266499.0A CN104073359A (en) | 2014-06-16 | 2014-06-16 | Process for extracting alpinia oxyphylla volatile oil through biological enzyme-supercritical fluid method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105542966A (en) * | 2016-01-29 | 2016-05-04 | 常州达奥新材料科技有限公司 | Method for extracting high-yield citrus essential oil |
| CN105907736A (en) * | 2016-05-05 | 2016-08-31 | 王美岭 | Supercritical CO2 ginger oil extraction method and special composite catalyst thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
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2014
- 2014-06-16 CN CN201410266499.0A patent/CN104073359A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105542966A (en) * | 2016-01-29 | 2016-05-04 | 常州达奥新材料科技有限公司 | Method for extracting high-yield citrus essential oil |
| CN105907736A (en) * | 2016-05-05 | 2016-08-31 | 王美岭 | Supercritical CO2 ginger oil extraction method and special composite catalyst thereof |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
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