CN104068823B - In-vivo microendoscopic spectral imaging system - Google Patents

In-vivo microendoscopic spectral imaging system Download PDF

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CN104068823B
CN104068823B CN201410347433.4A CN201410347433A CN104068823B CN 104068823 B CN104068823 B CN 104068823B CN 201410347433 A CN201410347433 A CN 201410347433A CN 104068823 B CN104068823 B CN 104068823B
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imaging system
light source
vivo
microendoscopic
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CN104068823A (en
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张红明
张立福
岑奕
吴太夏
黄长平
李瑶
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Feng Yanlong
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Institute of Remote Sensing and Digital Earth of CAS
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Abstract

The invention belongs to the technical field of optical imaging, and particularly relates to an in-vivo microendoscopic spectral imaging system. The in-vivo microendoscopic spectral imaging system comprises a light source unit, a light split unit, an endoscopic unit, a dispersion unit, a detection unit and a computer unit, wherein the dispersion unit comprises a dispersion device and a dispersion device controller which controls the dispersion device to achieve gating of signals with different wave lengths; and the dispersion device adopts an acousto-optic adjustable filter, a liquid-crystal adjustable filter or a thin-film adjustable filter. The in-vivo microendoscopic spectral imaging system is relatively simple in structure, smaller in size and more convenient to use; subcellular-resolution imaging can be performed on the inner surface of an in-vivo tract organ through introduction of an imaging optical fiber bundle; and more importantly, the dispersion device adopts a full-seal design and does not have a movable part, so that the environmental adaptability of the equipment is improved greatly, and the system works more stably.

Description

A kind of live body microscopy endoscopic spectrum imaging system
Technical field
The invention belongs to optical image technology field, particularly relate to a kind of live body microscopy endoscopic spectrum Imaging system.
Background technology
Tumor is the major disease of serious threat human health.Nearly 30 years, whole world cancer (was disliked Property tumor) morbidity number with average annual 3%~5% speed increase, it is most important that cancer has become the mankind One of the cause of the death.Clinical experience shows that the more early healing survival rate of the diagnosis to cancer is the highest, in It is that by the micro-endoscope technology of the high-resolution imaging close to biopsy increasingly Come into one's own.Traditional formation method, the most all only has imaging diagnosis can only provide space shape State information.And spectroscopic diagnostics is dependent on the acquisition optical radiation signal at certain wavelength to distinguish sample The information of product biochemical composition, obtains the curve of spectrum of each each pixel of image, Spectroscopic diagnostics can also provide basis for estimation accurately to medical diagnosis on disease.Simultaneously by microscopy endoscopic Optical image technology and high light spectrum image-forming technology combine and for studying biology and medical science Problem is also a new focus of development in recent years, uses spectrum change to carry out disease in clinic Sick diagnosis have also been obtained and uses widely.Imaging diagnosis is combined with spectroscopic diagnostics, same Realize the acquisition of the two on endoscope system simultaneously, greatly improve the sensitivity of detection, both obtained Obtain the sample morphology information of subcellsular level, the sample biochemistry of molecular level can be provided again Composition information, it has also become one of foundation accurately judging disease.
But spectrum imaging system of the prior art is considerably complicated, bulky, cost is held high Expensive, and due to stability, resolution, precision and easily by problems such as surrounding environment influences Often it is extremely difficult to the requirement of Precise Diagnosis so that existing microscopy endoscopic imaging diagnosis and spectrum Learn diagnosis combination and be extremely difficult to ideal effect.
Summary of the invention
For defect of the prior art, the present invention provides a kind of live body microscopy endoscopic light spectrum image-forming System, it is possible to convenient accurate acquisition intracoelomic cavity road organ inner surface subcellular definition Image information and the spectral information of nanometer resolution.
Live body microscopy endoscopic spectrum imaging system of the present invention, including light source cell, light splitting list Unit, in peep unit, dispersion element, probe unit and computer;
Described light source cell connects spectrophotometric unit, and described spectrophotometric unit peeps unit and color with interior respectively Throwaway unit connects, and described dispersion element is also respectively connected with probe unit and computer, described spy Survey unit and be also connected to computer;
Described dispersion element includes Dispersive Devices and Dispersive Devices controller, described Dispersive Devices control Device processed controls Dispersive Devices and realizes the gating of different wave length signal;Described Dispersive Devices is that acousto-optic can Light filter (Acousto-Optic Tunable Filter is called for short AOTF), liquid crystal tunable is adjusted to filter Sheet or thin film adjustable optical filter.
Further, peep unit in described and include imaging lens, microcobjective and imaging optical fiber bundle; Described imaging lens and microcobjective composition contracting beam system.
Further, described light source is white light source or LASER Light Source;Described spectrophotometric unit is for dividing Bundle device or dichroic mirror.
Further, described probe unit be charge coupled cell (Charge-coupled Device, It is called for short CCD) detector.
Live body microscopy endoscopic spectrum imaging system of the present invention, constructs relatively easy, small volume, Use convenient;The introducing of imaging optical fiber bundle can carry out Asia to intracoelomic cavity road organ inner surface The imaging of cellular resolution, it is often more important that owing to Dispersive Devices uses hermetically sealed design, without moving Dynamic component, substantially increases the environmental suitability of instrument so that system work is more stable, and And being greatly improved along with precision and resolution, the endoscopic picture of subcellular definition can be obtained Information and the spectral information of nanometer resolution, the system such as assist digestion that can be strong and urinary system is each The diagnosis of item disease.
Accompanying drawing explanation
By being more clearly understood from the features and advantages of the present invention with reference to accompanying drawing, accompanying drawing is to show Meaning property and should not be construed as the present invention is carried out any restriction, in the accompanying drawings:
Fig. 1 is microscopy endoscopic spectrum imaging system structural representation of the present invention;
Detailed description of the invention
In conjunction with drawings and Examples, technical solution of the present invention is further elaborated.
As it is shown in figure 1, embodiment of the present invention endoscopic spectrum imaging system includes light source cell, divides Light unit, in peep unit, dispersion element, probe unit and computer.
Described light source cell connects spectrophotometric unit, and described spectrophotometric unit peeps unit and color with interior respectively Throwaway unit connects, and described dispersion element is also respectively connected with probe unit and computer, described spy Survey unit and be also connected to computer;
Described dispersion element includes Dispersive Devices and Dispersive Devices controller, described Dispersive Devices control Device processed controls Dispersive Devices and realizes the gating of different wave length signal;Described Dispersive Devices is that acousto-optic can Adjust light filter, liquid crystal tunable optical filter or thin film adjustable optical filter.
Light source cell is used for providing detection light beam.Wherein, when realizing reflectance spectrum detection, light Source unit can be wide spectrum light source;When realizing fluorescence spectrum detection, light source cell can be sharp Radiant.
Spectrophotometric unit provides two paths;The detection light beam of one route light source cell output is through light splitting Unit is peeped in unit in entering;Another road from interior peep unit return reflection light beam through spectrophotometric unit Rear entrance dispersion element.
Wherein, if detecting reflectance spectrum, spectrophotometric unit is beam splitter, if Detecting fluorescence spectrum, spectrophotometric unit is then dichroic mirror.
Inside peep unit and include imaging lens, microcobjective and imaging optical fiber bundle;Imaging lens is with aobvious Speck mirror composition contracting beam system, for contracting to the detection light beam received from spectrophotometric unit Bundle, imaging optical fiber bundle is for conducting the light beam after contracting bundle to detecting sample, and collects by detecting The fluorescence signal of sample return or reflected light signal.
Such as, the present embodiment live body microscopy endoscopic spectrum imaging system is used to carry out medical diagnosis on disease Time, light source cell send wide range light beam or laser beam, and enter spectrophotometric unit, light splitting Unit peeps unit in wide range light beam or laser beam being reflexed to.Inside peep the imaging lens in unit With the contracting beam system of microcobjective composition, wide range light beam or laser beam contracting bundle are arrived and imaging The degree that fine bundle physical size is suitable;Microcobjective by wide range light beam or laser beam focusing to becoming As the front end face of fibre bundle, by the conduction of imaging optical fiber bundle, wide range light beam or laser beam are passed It is passed to detect at sample.The actually used middle imaging optical fiber bundle biopsy channel by conventional endoscope Enter into the tract organ inner surfacies such as internal digestive system or urinary system.Wide range light beam or sharp Light light beam returns diffuse-reflectance signal or fluorescence signal after arriving detection sample, and Returning beam passes through The collection of imaging optical fiber bundle, then via microcobjective and the collimator and extender of imaging lens, through dividing Light unit arrives dispersion element;Dispersive Devices controller in dispersion element controls Dispersive Devices Wavelength gated nature, wherein Dispersive Devices is acousto-optic turnable filter, the selected different wavelengths of light of order Bundle passes through, and allows that the signal of a wavelength passes through every time, and the light beam of the logical narrow-band of band passes through dispersion Focus on the detection unit after device, the probe unit record image by wavelength light beam.
In vivo peeped spectrum imaging system by the present embodiment, construct relatively easy, easy to use, Little by surrounding environment influence, to more square in the detection of soma's tract organ inner surface at body Just, testing result is more accurate;Cost is the cheapest.The introducing of imaging optical fiber bundle can be right Intracoelomic cavity road organ inner surface carries out the imaging of subcellular definition, and can obtain subcellular fraction The endoscopic picture information of resolution and the spectral information of nanometer resolution, can strong auxiliary disappear The diagnosis of the every diseases of system such as change and urinary system.
Although being described in conjunction with the accompanying embodiments of the present invention, but those skilled in the art can To make various modifications and variations without departing from the spirit and scope of the present invention, so Amendment and within the scope of modification each falls within and is defined by the appended claims.

Claims (3)

1. a live body microscopy endoscopic spectrum imaging system, it is characterised in that described system bag Include light source cell, spectrophotometric unit, in peep unit, dispersion element, probe unit and computer;
Described light source cell connects spectrophotometric unit, and described spectrophotometric unit peeps unit and color with interior respectively Throwaway unit connects, and described dispersion element is also respectively connected with probe unit and computer, described spy Survey unit and be also connected to computer;
Described dispersion element includes Dispersive Devices and Dispersive Devices controller, described Dispersive Devices control Device processed controls Dispersive Devices and realizes the gating of different wave length signal;Described Dispersive Devices is that acousto-optic can Adjust light filter AOTF, liquid crystal tunable optical filter or thin film adjustable optical filter;
Described light source cell is LASER Light Source;Described spectrophotometric unit is dichroic mirror.
Live body microscopy endoscopic spectrum imaging system the most according to claim 1, its feature Be, described in peep unit and include imaging lens, microcobjective and imaging optical fiber bundle;Described one-tenth As camera lens and microcobjective composition contracting beam system.
Live body microscopy endoscopic spectrum imaging system the most according to claim 2, its feature Being, described probe unit is charge coupled cell ccd detector.
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CN108245121A (en) * 2016-12-29 2018-07-06 刘小华 Endoscope and its imaging method and intelligent diagnosis system
CN107361724A (en) * 2017-07-20 2017-11-21 南京亘瑞医疗科技有限公司 Tomography endoscopic microscopic imaging device
CN110478625B (en) * 2019-08-21 2020-12-08 中国科学院苏州生物医学工程技术研究所 Precise photodynamic treatment system based on hyperspectral and fluorescence imaging guidance
CN111579498B (en) * 2020-04-26 2021-08-06 中国科学院苏州生物医学工程技术研究所 Hyperspectral endoscopic imaging system based on push-broom imaging
CN113440093B (en) * 2021-07-19 2022-11-25 山东第一医科大学附属省立医院(山东省立医院) Digestive tract secretion microscopic detection system

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