CN104067923A - Cultivation method for high protein and high vitamin C content radish seed sprouts - Google Patents

Cultivation method for high protein and high vitamin C content radish seed sprouts Download PDF

Info

Publication number
CN104067923A
CN104067923A CN201410353011.8A CN201410353011A CN104067923A CN 104067923 A CN104067923 A CN 104067923A CN 201410353011 A CN201410353011 A CN 201410353011A CN 104067923 A CN104067923 A CN 104067923A
Authority
CN
China
Prior art keywords
milk
radish seed
content
radish seeds
radish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410353011.8A
Other languages
Chinese (zh)
Other versions
CN104067923B (en
Inventor
葛喜珍
赵莹
王玥
张元�
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Union University
Original Assignee
Beijing Union University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Union University filed Critical Beijing Union University
Priority to CN201410353011.8A priority Critical patent/CN104067923B/en
Publication of CN104067923A publication Critical patent/CN104067923A/en
Application granted granted Critical
Publication of CN104067923B publication Critical patent/CN104067923B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y02P60/216—

Landscapes

  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a cultivation method for high protein and high vitamin C content radish seed sprouts. The method comprises the following steps: selecting vermiculite as a cultivation medium, taking fresh and full radish seeds, soaking the radish seeds in warm water at 50-55 DEG C for 15 min, taking the radish seeds out, soaking the radish seeds in a 1% potassium permanganate solution for 15 min, taking out the radish seeds, and washing the radish seeds twice with clear water; uniformly broadcasting the radish seeds on the medium, and covering the radish seeds with 1.5-2.0 cm of vermiculite; sowing the radish seeds, preparing a 0.5-2 g/L water solution of milk, pouring the water solution of milk into the culture medium, controlling the humidity at 50-60%, and cultivating the radish seeds at 20-30 DEG C for 5-7 days, and harvesting. The method provided by the invention is suitable for intensive cultivation of high protein and high vitamin C content radish seed sprouts; the process feasibility is high, and the sprouts are free of influence from external environment in the production process and don't need chemical fertilizer, hormones and agricultural chemicals; the field and nutrient fluid costs are low, the investment is small, the efficiency is high, the growth cycle is short, the yield and the product added value are high, and the method can be used for industrial and intensive production of sprouts.

Description

The breeding method of a kind of high protein, homovitamin C content radish seed bud dish
Technical field
The present invention relates to a kind of breeding method of radish seed bud dish, relate in particular to the breeding method of a kind of high protein, homovitamin C content radish seed bud dish, belong to agricultural production, raw-food material production field.
Background technology
Bud class is traditional high quality food that compatriots like.Bud-genus food refers generally to the food that cereal, beans are produced through germination, commonly moyashi, mung bean sprouts.The nutritive value of bud dish is higher, because its vegetative period is short, does not need fertilising, without just harvest well of laxative, is non-harmful vegetables.Radish seed, another name SEMEN RAPHANI, trailing plants white chessman, dish chieftain, Latin literary fame Semen Raphani., is the mature seed of crucifer radish, is used for the treatment of retention of food and drink, abdominal distention, constipation, the stagnant dysentery, phlegm of rushing down is stopped up and breathed with cough etc.In radish seed bud dish, contain abundant nutriment, vitamin content is equivalent to 10 times of Chinese cabbage.Radish seed bud dish is slightly with pungent taste, edible very easy, therefore can go deep into rapidly general family, the most important thing is that it can water planting, and plantation is convenient.In recent years, people like adding bud dish in eating barbecue, western-style food or salad, not only good to eat but also nutrition.As added ternip seedling to nurse one's health in the lump at taste noise juice or with meat, not only can promote stomach digestion, and can eliminate greasy.
Summary of the invention
The object of the present invention is to provide the breeding method of a kind of high protein, homovitamin C content radish seed bud dish, radish seed germination condition is screened, to increase radish seed germination rate, improve albumen, the vitamin content of radish seed bud dish, so that high-quality radish bud dish to be provided.
For achieving the above object, the present invention is by the following technical solutions:
A breeding method for high protein, homovitamin C content radish seed bud dish, the method comprises the following steps:
(1) select culture medium: take vermiculite as culture medium, pack in nutrient discs, the thickness of vermiculite is 5-7cm;
(2) seed selection: select fresh full radish seed, used 50-55 ℃ of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water is washed 2 times;
(3) sowing: seed uniform broadcasting, in matrix, is covered to vermiculite 1.5-2.0cm;
(4) add culture fluid: milk is mixed with to the 0.5-2g/L aqueous solution, pours milk soln into culture matrix, humidity is agglomerating to hold with hand, stretch out one's hand and fall apart and be advisable;
(5) management: cultivate 5-7 days, results in 20-30 ℃.
It is culture matrix that the present invention selects vermiculite, and vermiculite is a kind of natural, nontoxic mineral matter, dilatable mineral under high temperature action.Because vermiculite has the ability of ion exchange, it has great effect to the nutrition of soil.Vermiculite can promote the growth of root system of plant and the stable growth of seedling effectively.The necessary moisture of plant growth and nutrition are provided for a long time, and can keep the stable of plant root temperature.Vermiculite can make crop just can obtain sufficient moisture and mineral matter from early growth period, promotes plant faster growing, increases output.
Preferably, described nutrient discs is placed on semi-enclosed layer stereo support, and so-called semi-enclosed layer stereo support is three side sealing and closes, the layer stereo support that top, bottom and operating surface are exposed.This semi-enclosed layer stereo support, both for putting multilayer seedling dish, carries out vertical cultivation, both can improve and utilize space, cost-saving, contributes to again the humidity that keeps certain.
Preferably, in described step (4), the humidity of culture matrix is controlled at 50-60%.
Preferably, the concentration of the aqueous solution of described milk is 1g/L; In described step (5), cultivation temperature is 20 ℃, and incubation time is 7 days.
The present invention has broken through the traditional mode of production pattern of radish seed (SEMEN RAPHANI) bud dish, has initiated and under mal-condition, has utilized simple machine to carry out novel standardization intensive production new model.Beneficial effect of the present invention is:
1, the present invention is suitable for intensive cultivation high protein, homovitamin C content radish seed bud dish.
2, technological feasibility of the present invention is strong, is not affected by the external environment, without chemical fertilizer, hormone and agricultural chemicals in the production process of bud seedling dish.
3, the present invention place used and nutrient solution cost are low, invest little, instant effect, growth cycle is short, output is high, and added value of product is high, can be used for the batch production of bud dish, intensive production.
4, the Product Safety that adopts the present invention to cultivate is high, and environmentally safe, has really met people to natural, nuisanceless, high-quality demand, and the yearning to pure life is the green organic products that meets current era meaning.
Embodiment
Below by specific embodiment, the present invention will be further described.
In following examples, the mensuration of protein content and the mensuration of Vitamin C content adopt respectively following methods.
1, by Coomassie brilliant blue method, measure protein content
(1) preparation of standard liquid: accurately take bovine serum albumin(BSA) 100.00mg, distilled water dissolves, and is settled to 1000mL, and this is 0.1g/L protein standard reserving solution, Refrigerator store.
(2) preparation of Coomassie brilliant blue G-250 solution: precision takes Coomassie brilliant blue G-250100.00mg, adds 95% ethanol 50mL (blueness), then adds 85% (W/V) H 3pO 4100mL (blood red) is finally settled to 1000mL (brown) with distilled water.
(3) preparation of need testing solution: take drying sample 0.5g, add a small amount of quartz sand and grind to form homogenate with distilled water and be settled to 100mL, standing 10-30min (3-5min shakes once) filters, and it is standby that filtrate is settled to 100mL.
(4) Specification Curve of Increasing: standard liquid 1mL, add after Coomassie brilliant blue G-250 protein reagent, shake up, to place after 2min, colorimetric estimation under 595nm wavelength, records A595.The protein content (X) of take be take absorbance (A) as abscissa and is carried out linear regression analysis as ordinate, and equation of linear regression is: A=1.565X-0.005, coefficient R 2=0.999.
(5) sample size is measured: get 2, test tube, and 1 preparation 1mL liquid to be measured that adds need testing solution, 1 adds 1mL distilled water (doing blank), add again 5.0mL Coomassie brilliant blue G-250 reagent, shake up, place after 5min, colorimetric under 595nm wavelength, records A595.Press Specification Curve of Increasing operation, measure absorbance, record data, calculate protein content.
2, the mensuration of Vitamin C content
The drafting of calibration curve is drawn respectively 1g/L vitamin C titer 0.00mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL and 1.00mL to 6 clean 50mL test tube with pipette, be settled to scale, the 0.00mL vitamin C mark liquid of take is made reference as blank, under 267nm wavelength, use 1cm quartz colorimetric utensil, measure absorbance.The mass concentration of vitamin C series solution of take is abscissa, take corresponding absorbance as ordinate, draws out calibration curve.Data are returned to processing, and result shows, ascorbic mass concentration is linear within the scope of 0～20mg/L, and equation of linear regression is: A=0.06163X-0.02934, coefficient R 2=0.998.
3, ascorbic extraction and mensuration in radish seed sample
Bud dish is cleaned, drained, chopping, mixes, and accurately takes respectively 10g in grinding alms bowl, adds the hydrochloric acid of 10mL1%, and homogenate moves in 50mL volumetric flask, with distilled water diluting to scale.Difference accurate measuring 1mL extract, wherein 1mL puts into the 50mL test tube that fills 2mL10% hydrochloric acid, measures it in 267nm place absorbance.By calibration curve, calculate Vitamin C content.
Embodiment
1, seed sprouting pre-treatment
The germination of seed and pre-treatment, select that color and luster is fresh, full seed, free from insect pests, without going rotten, without broken beans, high quality seed that germination vigor is strong, with clear water rinsing 2-3 time, 50-55 ℃ of emerge in worm water 15min for seed, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water is washed 2 times, stand-by.
2, select culture medium
Selecting vermiculite, fine sand, 3 kinds of seedling mediums of light soil is culture medium, packs nutrient discs into, stromal thickness 5-7cm, and nutrient discs is put to semi-enclosed layer stereo support.Pour water into culture matrix, the humidity of controlling culture matrix is the seed sprouting number of observing its germination vigor for 60%, 5 day and recording every group, measures average height, weightless dry weight, calculates germination rate, and upgrowth situation is in Table 1.
Table 1 different substrates germinates and affects (100 seeds) radish seed
Culture matrix Fine sand Vermiculite Light soil
Number sprouts 93 98 92
Bud ratio 93％ 98％ 92％
Bud dish average height 7.5cm 9.2cm 8.2cm
Weight in wet base 5.235g 6.668g 5.870g
Dry weight 0.278g 0.323g 0.293g
As can be seen from Table 1, take vermiculite as matrix radish seed germination rate and dry weight in wet base all higher, selection vermiculite is culture matrix.
3, temperature, milk concn dialogue radish seed growth effect
Under 15 ℃, 20 ℃, 25 ℃, 30 ℃ constant temperatures, add respectively nutrient solution (milk or water), add variable concentrations milk soln, carry out germination test.Wherein vermiculite test, by seed uniform broadcasting in matrix, covers vermiculite 1.5cm.Milk concn is the 1.0g-10/L aqueous solution, pour milk soln into culture matrix, the humidity of controlling culture matrix is within 60%, the 5 day, observe its germination vigor and record the seed sprouting number of every group, measure average height, weightless dry weight, calculate germination rate, upgrowth situation is in Table 2-5.
Table 2 radish seed is at 15 ℃ of germination (100 seeds)
Culture fluid Add water 0.5g/L milk 1g/L milk 5g/L milk 10g/L milk
Number sprouts 93 92 97 95 91
Bud ratio 93％ 92％ 97％ 95％ 91％
Bud dish average height 8.3cm 8.3cm 8.8cm 8.7cm 8.7cm
Weight in wet base 5.962 6.077g 6.522g 6.136g 5.871g
Dry weight 0.302 0.301g 0.316g 0.311g 0.305g
By table 2, drawn: according to bud ratio, bud dish average height, and moisture content compares and draw, at 15 ℃ of temperature, take vermiculite as culture matrix, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 3 radish seed is at 20 ℃ of germination (100 seeds)
Add culture fluid Add water 0.5g/L milk 1g/L milk 5g/L milk 10g/L milk
Number sprouts 90 94 92 90 88
Bud ratio 90％ 94％ 92％ 90％ 88％
Bud dish average height 8.3cm 8.4cm 9.2cm 8.8cm 8.1cm
Weight in wet base 5.422g 6.138g 6.436g 6.024g 5.631g
Dry weight 0.306g 0.302g 0.318g 0.336g 0.311g
By table 3, drawn: according to bud ratio, bud dish average height, and moisture content compares and draw, at 20 ℃ of temperature, take vermiculite as culture matrix, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 4 radish seed is at 25 ℃ of germination (100 seeds)
Add culture fluid Add water 0.5g/L milk 1g/L milk 5g/L milk 10g/L milk
Number sprouts 91 92 93 90 88
Bud ratio 91％ 92％ 93％ 90％ 88％
Bud dish average height 8.5cm 8.8cm 9.1cm 8.6cm 8.4cm
Weight in wet base 5.386g 6.358g 6.524g 6.109g 5.620g
Dry weight 0.312g 0.317g 0.338g 0.341g 0.302g
By table 4, drawn: according to bud ratio, bud dish average height, and moisture content compares and draw, at 25 ℃ of temperature, take vermiculite as culture matrix, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 5 radish seed is at 30 ℃ of germination (100 seeds)
Culture fluid Add water 0.5g/L milk 1g/L milk 5g/L milk 10g/L milk
Number sprouts 93 98 97 95 91
Bud ratio 93％ 98％ 97％ 95％ 91％
Bud dish average height 9.2cm 9.9cm 9.7cm 9.0cm 8.7cm
Weight in wet base 5.962g 6.321g 6.522g 6.136g 5.871g
Dry weight 0.322g 0.329g 0.346g 0.331g 0.305g
By table 5, drawn: according to bud ratio, bud dish average height, and moisture content compares and draw, at 30 ℃ of temperature, take vermiculite as culture matrix, adding 0.5g/L milk is that the bud dish growing state of culture fluid is best.
Sum up: according to table, 1-5 draws, radish seed bud dish, at 20-30 ℃, be take vermiculite as culture matrix, and the milk that concentration is 1g/L is that culture fluid bud dish growing state is good.
4, incubation time dialogue radish seed growth effect
Radish seed bud dish, at 25 ℃, be take vermiculite as culture matrix, and the milk that concentration is 1g/L is culture fluid, observes the impact of different incubation times on bud seedling output.Result is as shown in table 6.
The impact (100 seed) of table 6 incubation time on radish seed growth
Cultivated days (d) 2 4 6 8
Number sprouts 97 97 97 97
Bud ratio 97％ 97％ 97％ 97％
Bud dish average height 2.3cm 6.1cm 9.2cm 9.7cm
Weight in wet base 3.242g 5.380g 7.098g 7.256g
Dry weight 0.208g 0.315g 0.360g 0.371g
According to bud ratio, bud dish average height and dry weight in wet base, with incubation time, increasing output increases, and considers manpower and material cost, should cultivate 5-7 days.
5, temperature, the impact of incubation time on radish seed protein content
Take vermiculite as culture matrix, and the milk that concentration is 1g/L is culture fluid.Different temperatures, cultivation different time radish seed protein content change.Result is as shown in table 7.
Table 7 temperature, incubation time are on the impact of radish seed protein content (mg/g)
By table 7, drawn: the content at the protein of 20 ℃ of bud dishes is high, cultivates 1-2 days protein contents high, consider bud dish output (dry, weight in wet base), should cultivate 5-7 days.
6, milk soln and the water impact on protein content
Take vermiculite as culture matrix, 20 ℃ of cultivations, the milk that concentration is 1g/L is culture fluid and the impact of water on protein content.Result is as shown in table 8.
Table 8 milk soln and water are on the impact of protein content (mg/g)
By table 8, drawn: under equal conditions, milk soln can obviously improve bud dish protein content.
7, radish seed Vitamin C content (mg/100g) is measured orthogonal experiment
Take vermiculite as culture matrix, and temperature, milk concn, cultivation number of days are influence factor, design 3 levels, observe the variation of Vitamin C content.Result is as shown in table 9.
Table 9 radish seed Vitamin C content (mg/100g) is measured orthogonal experiment
Horizontal factor Temperature (℃) Milk concn (g/L) Number of days (D)
1 19 0 4
2 20 1 6
3 21 1.1 8
Select L9 (34) orthogonal table, the 4th row can be arranged without factor, as sky row, do not arrange factor.According to orthogonal table requirement, carry out nine experiments, using Vitamin C content height as investigating index, the results are shown in Table 10.
Table 10 radish seed Vitamin C content (mg/100g)
Column 1 2 3 4 ?
Factor Temperature Milk concn Number of days Empty row Experimental result
Experiment 1 1 1 1 1 48.96
Experiment 2 1 2 2 2 52.32
Experiment 3 1 3 3 3 52.38
Experiment 4 2 1 2 3 60.14
Experiment 5 2 2 3 1 56.44
Experiment 6 2 3 1 2 47.21
Experiment 7 3 1 3 2 46.79
Experiment 8 3 2 1 3 51.46
Experiment 9 3 3 2 1 50.09
Average 1 52.200 51.963 49.210 51.830 ?
Average 2 54.597 54.407 55.183 49.773 ?
Average 3 49.447 49.873 51.850 54.640 ?
Extreme difference 5.150 14.534 5.973 4.867 ?
Relatively the extreme difference value of each factor can be seen, the growth temperature that radish seed Vitamin C content is the highest is 20 ℃, and the concentration of culture fluid milk is 1g/L.Best growth number of days is 7 days.

Claims (5)

1. a breeding method for high protein, homovitamin C content radish seed bud dish, is characterized in that, the method comprises the following steps:
(1) select culture medium: take vermiculite as culture medium, pack in nutrient discs, the thickness of vermiculite is 5-7cm;
(2) seed selection: select fresh full radish seed, used 50-55 ℃ of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water is washed 2 times;
(3) sowing: seed uniform broadcasting, in matrix, is covered to vermiculite 1.5-2.0cm;
(4) add culture fluid: milk is mixed with to the 0.5-2g/L aqueous solution, pours milk soln into culture matrix, humidity is agglomerating to hold with hand, stretch out one's hand and fall apart and be advisable;
(5) management: cultivate 5-7 days, results in 20-30 ℃.
2. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, described nutrient discs is placed on semi-enclosed layer stereo support.
3. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, in described step (4), the humidity of culture matrix is controlled at 50-60%.
4. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, the concentration of the aqueous solution of described milk is 1g/L.
5. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, in described step (5), cultivation temperature is 20 ℃, and incubation time is 7 days.
CN201410353011.8A 2014-07-23 2014-07-23 The breeding method of a kind of high protein, homovitamin C content radish seed bud dish Expired - Fee Related CN104067923B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410353011.8A CN104067923B (en) 2014-07-23 2014-07-23 The breeding method of a kind of high protein, homovitamin C content radish seed bud dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410353011.8A CN104067923B (en) 2014-07-23 2014-07-23 The breeding method of a kind of high protein, homovitamin C content radish seed bud dish

Publications (2)

Publication Number Publication Date
CN104067923A true CN104067923A (en) 2014-10-01
CN104067923B CN104067923B (en) 2016-01-20

Family

ID=51589853

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410353011.8A Expired - Fee Related CN104067923B (en) 2014-07-23 2014-07-23 The breeding method of a kind of high protein, homovitamin C content radish seed bud dish

Country Status (1)

Country Link
CN (1) CN104067923B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104855103A (en) * 2015-05-22 2015-08-26 枞阳县白云生态园林有限责任公司 Seedling growing method for semen raphani
CN111937730A (en) * 2019-05-14 2020-11-17 临洮县兴望牡丹产业有限责任公司 Purple-spot peony bud cultivation method and cultivation system thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009225774A (en) * 2008-03-25 2009-10-08 Noriomi Watanabe Japanese ginger-cultivating method having nutrient solution circulation
CN102630548A (en) * 2012-03-30 2012-08-15 江苏田娘农业科技有限公司 Soil-free cultivation method of radish sprout
CN103385098A (en) * 2013-07-08 2013-11-13 安徽金麒麟农业科技有限公司 Turnip sprout vegetable soilless cultivation method
CN103385162A (en) * 2012-05-10 2013-11-13 王瑞明 Soilless cultivation method for radish sprouts
CN103609306A (en) * 2013-11-26 2014-03-05 青岛东颐锦禾农业科技有限公司 Turnip sprout soilless culture technology
CN103907511A (en) * 2013-01-05 2014-07-09 赵百华 Radish seedling high-yield cultivation technology

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009225774A (en) * 2008-03-25 2009-10-08 Noriomi Watanabe Japanese ginger-cultivating method having nutrient solution circulation
CN102630548A (en) * 2012-03-30 2012-08-15 江苏田娘农业科技有限公司 Soil-free cultivation method of radish sprout
CN103385162A (en) * 2012-05-10 2013-11-13 王瑞明 Soilless cultivation method for radish sprouts
CN103907511A (en) * 2013-01-05 2014-07-09 赵百华 Radish seedling high-yield cultivation technology
CN103385098A (en) * 2013-07-08 2013-11-13 安徽金麒麟农业科技有限公司 Turnip sprout vegetable soilless cultivation method
CN103609306A (en) * 2013-11-26 2014-03-05 青岛东颐锦禾农业科技有限公司 Turnip sprout soilless culture technology

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
何伟明等: "芽苗菜栽培新模式——苗菜穴盘栽培", 《中国蔬菜》 *
崇明蔬菜站: "种芽苗菜 把"菜园"带回家", 《上海农业技术网HTTP://WWW.AGRI.SH.CN/NJXW/NYXW/201012/T20101203_169410.HTML》 *
张利民等: "芽苗菜周年生产栽培技术", 《农牧产品开发》 *
毛久庚等: "萝卜芽苗菜有机基质快繁技术研究", 《现代农业科技》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104855103A (en) * 2015-05-22 2015-08-26 枞阳县白云生态园林有限责任公司 Seedling growing method for semen raphani
CN111937730A (en) * 2019-05-14 2020-11-17 临洮县兴望牡丹产业有限责任公司 Purple-spot peony bud cultivation method and cultivation system thereof

Also Published As

Publication number Publication date
CN104067923B (en) 2016-01-20

Similar Documents

Publication Publication Date Title
CN103461861A (en) Preparation method of highland barley milled rice with embryos
CN101283667B (en) Method for cultivating iron-rich bud seeding vegetable
CN101138300B (en) Euryale ferox artificial cultivating method
CN107079700A (en) A kind of method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area
CN105706875A (en) Production method for moringa oleifera sprouting vegetables
CN104067923B (en) The breeding method of a kind of high protein, homovitamin C content radish seed bud dish
CN102499034B (en) Soilless cultivation method capable of reducing content of nitrate in curled mallow and curled mallow nutrient solution
CN104041654B (en) A kind of tissue culture method produces the method for selenium-rich rice albumen
CN109429618A (en) A method of improving corn germination rate
CN105493993A (en) Rapid cultivation method of macadamia nut rootstocks
CN107155665A (en) A kind of method for strategy of being poured water based on leaf area model of growth formulation crop
Tlevlessova et al. Mechanisation of the primary processing of watermelons without destroying the rind
Amirnia et al. Influence of corm provenance and sowing dates on stigma yield and yield components in saffron (Crocus sativus L.)
CN106282034A (en) A kind of Morchella esculenta (L.) Pers strain fast breeding method
CN103404340B (en) Cultivation method of sweet potato used for processing mashed potato and processing method of sweet potato
CN105557643A (en) Method for artificially and massively propagating experimental green peach aphids
CN109729780A (en) A method of improving germination of stevia rebaudiana seeds rate
CN109392645B (en) Method for screening peanuts with high oil content by using osmotic pressure
CN105660335B (en) A kind of method and system for identifying moisture-proof barley variety
CN104351503B (en) A kind of green plant bug man-made feeds
Bovi et al. Adequate timing for heart-of-palm harvesting in King palm
CN103918378B (en) Seed processing method for improving the capability of soybean for resisting saline-alkali stress
CN106465746A (en) A kind of green corn preservation method
Jain et al. Economics of Foxnut (Euryale ferox Salisb.) cultivation: a case study from Manipur in North eastern India
CN103098699A (en) Breeding of new variety of disease-resistant green fruity radish with high yield

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160120

Termination date: 20180723