CN104056052B - Preparation process of powerful anti-aging Chinese patent medicine with anti-aging effect - Google Patents
Preparation process of powerful anti-aging Chinese patent medicine with anti-aging effect Download PDFInfo
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Abstract
The invention discloses a preparation process of a powerful anti-aging Chinese patent medicine with an anti-aging effect. The powerful anti-aging Chinese patent medicine is prepared from fructus lycii, glossy privet fruits, semen cuscutae, herba epimedii, astragalus membranaceus, salviae miltiorrhizae, hawthorn, spina date seeds and cortex lycii radicis. The process mainly comprises the following steps: percolating angelica sinensis by ethanol, collecting percolate and collecting filter residues in another container; carrying out water distillation on chrysanthemum to extract volatile oil, collecting the volatile oil, collecting the aqueous solution after distillation for later use and collecting filter residues in another container for later use; combining and decocting the filter residues with prepared polygonum multiflorum and sealwort, combining and concentrating the solution with the solution obtained in the above step, adding the volatile oil and uniformly mixing. The process improves the preparation process of the original traditional Chinese medicine composition and reduces the waste of raw materials, so that the extraction degree of the effective components in the traditional Chinese medicine composition is higher, and the obtained product is easy to dry, more stable and better in curative effect.
Description
Technical field
The present invention relates to a kind of preparation technology of Chinese patent medicine, particularly relate to a kind of preparation technology with the anti-ageing Chinese patent medicine of brute force of anti-aging effects.
Background technology
The degeneration change that aging is body tissue, organ dysfunction increases with the age and occurs, it is the general performance of the various biochemical reaction of body, the many factors in inside and outside, the coefficient results such as such as environmental pollution, psychentonia, heredity, finally process deathward.In October, 1999, China's more than 60 years old aging population have reached 1.26 hundred million people, account for 10% of total population, show that China has entered aged tendency of population country ranks.Then, aging population are by continuation with the speed increment of every annual more than 3%, and will reach 2.8 hundred million people by 2025, and account for 18% of total population, the year two thousand fifty reaches more than 400,000,000, accounts for 1/4 of total population, thus become the country of height aging.
Research about Aging mechanism is the advanced subject of biology, Gerontological Research Center always.At present, the old and feeble precise mechanism produced is not yet clear, researcheres proposed tens of kinds once about old and feeble theory, as free radical theory, DNA damage theory, tolemerase, cross linkage theory, biomembrane damage theory, genetic program theory, chromosome mutation theory, immunological theory, endocrine theory, apoptosis theory etc.But because this complex process old and feeble is by the coefficient result of several factors, also do not have a kind of theory can explain all aging phenomenons at present.
Chinese medicine, inquiring in old and feeble cause and mechanism, from ancient times to the present, proposes multiple different viewpoint and view, and the aging defining Chinese medicine uniqueness is theoretical.Ji Dunfu etc. propose causes of senescence and mainly contain above several on the basis of summing up TCM Document and each discussion: nativism, think that the senescence process of people and life-span depend on direct heredity basis; The day after tomorrow is said, think the senescence process of people and life-span depend primarily on conserve one's health the day after tomorrow whether proper; Deficient five internal organs is said, is one of theory had the greatest impact in the old and feeble theory of the traditional Chinese medical science; Imbalance of YIN and YANG is said, think old and feeble with occur in increasing age yin or yang excessly partially decline relevant, point out the importance etc. of equilibrium between yin and yang.
Simultaneously, have scholar think old and feeble except with congenital heredity and the health preserving day after tomorrow nurse i.e. father and mother hereditary basis and conserve one's health the day after tomorrow whether proper have outside the Pass, also has following several theory: the weak aging of the five internal organs is said: the traditional Chinese medical science thinks that within 40 years old, playing human body starts to occur old and feeble external change, begin from liver after 50 years old, with 10 years for the cycle, according to mutual generation of five phases rule, tend to old and feeble one by one, stereotype continues existing, and visible deficient five internal organs all can cause and decline; Aging of suffering from a deficiency of the kidney is said: the aging of people is first from kidney essense, and deficiency of kidney-essence then kidney yin kidney yang is also empty, the raw kidney qi of being difficult, and asthenia of renal qi then vital organs of the human body physiological function goes down, and occurs the performance of a series of aging.In addition, the kidney generating marrow and dominating bone, deficiency of kidney-essence then brain is hollow, causes vertigo and tinnitus, instability of gait.Therefore, suffering from a deficiency of the kidney is old and feeble main cause.The multi-disciplinary researchs such as modern neuro, endocrine, immunology also demonstrate that, suffer from a deficiency of the kidney to play an important role in human senility; Weakness of the spleen and stomach aging is said: The spleen and stomach provide the material basis of the acquired, source of generating QI and blood, if weakness of the spleen and stomach, then transportation capabilities go down, water paddyization spermatogenesis, gas, Tianjin, blood obstacle, insufficiency of vital energy and blood, phlegm-turbidity and blood stasis blood is stopped in body, internal organs, meridians, extremity bones of the body collectively lose in supplementing nutrition, thus lose normal physiological activity ability, cause aging.Modern age, the much research about aging also confirmed the important function of taste aging from molecular biology angle; Imbalance of YIN and YANG aging is said: the process of body aging is negative and positive disequilibriums, occurs the result that inclined Sheng declines partially or deficiency of both YIN and YANG causes.If further develop, negative and positive can not be refinforced each other and be separated, and the vital movement of people also just stopped.
Except several about except the theory of aging above, traditional Chinese medical science causes of senescence also has that stagnation of liver-QI aging is said, the turbid blood stasis of expectorant is old and feeble says.These theories only illustrate old and feeble problem in a certain respect and the old and feeble basic mechanism that is not sure.The traditional Chinese medical science thinks that suffering from a deficiency of the kidney is the basic reason of people's asthenia, is widely accepted when causing saying of declining to suffer from a deficiency of the kidney most in numerous theory.To suffer from a deficiency of the kidney that causing declines and be the vital essence loss, empty few of the yang-energy of nephrons and kidney Tibetan, the biochemical passive many old and feeble morbid state that causes of the five internal organs qi-blood-body fluid and aging course.Kidney storing essence is the congenital foundation, Source of life, and human body take kidney as all physiological functions that dominant force is presided over, maintained, make its uniform balance and can self-regulated from normal activity quietly, thus resist disease.Kidney qi virtual loss is the biochemical weary source of QI of five ZANG-organs blood body fluid then, and various senility disease just reveals day by day.Modern medicine study shows, suffering from a deficiency of the kidney of the traditional Chinese medical science causes old and feeble theory to have globality and comprehensive feature, it directly affects the aspects such as body neuroendocrine, free radical, immunologic function, thus causes the aspects such as old and feeble immunologic function, thus causes aging.
In recent years, the research and development of delay aging drug is very fast, and the research wherein about anti-aging compound recipe becomes focus gradually.From theory of Chinese medical science angle, Chinese medicine compound has irreplaceable advantage, has huge potentiality in slow down aging.The anti-aging effects of Chinese medicine compound is mainly realized by antioxidation, immunity moderation function, endocrine regulation function, mechanism such as adjustment nucleic acid metabolism and DNA repair ability etc.
Antioxidation: ERCHEN TANG can improve laboratory animal brain, thymus index, improves serum superoxide dismutases (SOD) active, reduces malonaldehyde (MDA) content, has certain anti-aging effects.Decoction of four noble drugs can improve laboratory animal SOD in serum and glutathion peroxidase (GSH-Px) is active, reduces MDA content, has certain anti-aging effects, for invigorating the spleen for dissipating phlegm defying age theory provides experimental basis.It is active that JINGUI SHENQI WAN can improve presenium Rat Erythrocytes SOD, and reduce rat blood serum MDA content, this change extends in aggravation trend with administration time.LIUWEI DIHUANG TANG can make immune organ Scavenging ability in yin-deficient model caused by corticosterone obviously strengthen, and the lipid peroxidation product attacked by free radical obviously reduces; The compound recipe with anti-radical action also has: ERZHI WAN, dihuang drink, add taste WEIDANTANG, HUANJINGJIAN, solid true capsule, Radix Notoginseng sheet, Poria SHENGMAI YIN, fleece-flower root long-life sheet, five-seed Yanzong side etc.
Immunity moderation function: LIUWEI DIHUANG TANG, pieces of Jin ' gui shenqi soup all can strengthen the vigor of immunocyte, but there are differences in the mechanism of action.DANGGUI SHAOYAO SAN has certain selectivity to immune impact, to strengthen cellular immune function, has certain facilitation to lymphopoiesis.Tiannianyin, by stoping the atrophy of spleen, promoting lymphocytic multiplication capacity, improves senile organism immunologic function, may have the effect of slow down aging.JINGUI SHENQI WAN can improve the phagocytic function of immunosuppressed mice peritoneal macrophage, can improve thymic weight, can improve hemolysin content, promotes the thin transformation function of lymph, improves RBC number, thus strengthens immunologic function.The compound recipe with Promote immunity function also has: Yougui Wan, the kidney-Yang-Reinforcing Bolus, compound recipe Radix Notoginseng soup, foster true oral liquid, HUANJINGJIAN etc.
Regulate neural and endocrine function: research finds, LIUWEI DIHUANG WAN is improved D-galactose by the function of coordination center cholinergic system and adrenergic system and is caused aging rats learning memory disorder.The party can also improve corticosterone and cause deficiency of the kidney yin mouse hypothalamus-Pituitary-ovary Axis dysfunction.Bolus as a Kidney-Yin-Tonic, Yougui Wan, the kidney-Yang-Reinforcing Bolus can delay the senile change of hypothalamo-pituitary-adrenal axis from organizational structure form aspect.The Chinese medicine compound neural and endocrine function to regulating action also has: kidney-tonifying life-prolonging capsule, five-seed Yanzong side, Chinese recipe etc.
Anti-DNA damnification: kidney and spleen invigorating nourshing blood and promoting blood circulation method prescription can reduce the oxidative damage of mitochondrial DNA, has protective effect, thus provide the mechanism of our slow down aging from molecular level to mitochondrial DNA.The Chinese medicines such as antiageing tablet fleece-flower root long-life sheet obviously can improve the senile symptom of aging model rat, and the ability that its mechanism of action may damage with raising body DNA plerosis is relevant.JINGUI SHENQI WAN has good antagonism to by caused by cyclophosphamide medullary cell DNA damage, thinks and improves the dominant mechanism place that body anti-medullary cell DNA damage ability may be JINGUI SHENQI WAN delaying senility function.In addition, WUZIYANZONG WAN and the side of tearing open, Kang Shou drink, LIUWEI DIHUANG WAN etc. all have the effect of anti-DNA damnification.
Application number is disclose a kind of anti-ageing Chinese patent medicine of brute force with anti-aging effects in the patent application of 200910079396.2, and this medicine mainly realizes slow down aging by mechanism such as antioxidation, immunity moderation function, endocrine regulation functions.
Its prescription is as follows:
Fructus Lycii 5 ~ 6 parts of Radix Polygoni Multiflori 6 ~ 7 parts of Fructus Ligustri Lucidi 5 ~ 6 parts of Radix Angelicae Sinensis 5 ~ 7 parts of Semen Cuscutae 10 ~ 12 parts of Herba Epimedii 4 ~ 6 parts of Radixs Astragali 7 ~ 9 parts of Rhizoma Polygonatis 10 ~ 12 parts of Radix Salviae Miltiorrhizaes 5 ~ 6 portions of Fructus Crataegis 8 ~ 9 parts of Semen Ziziphi Spinosaes 5 ~ 6 parts of Flos Chrysanthemis 11 ~ 12 parts of Cortex Lycii 5 ~ 6 parts
Its preparation technology is as follows:
1) get Radix Angelicae Sinensis and chrysanthemum with water is dipped to fully moistening, be heated to boil, and keep micro-and boil to well-done, filter Radix Angelicae Sinensis, Flos Chrysanthemi filtrate separately put preservation, the medicinal residues of Radix Angelicae Sinensis, Flos Chrysanthemi send next procedure;
2) Radix Angelicae Sinensis of above-mentioned gained, the medicinal residues of Flos Chrysanthemi and Radix Polygoni Multiflori, Radix Salviae Miltiorrhizae, Fructus Ligustri Lucidi, Cortex Lycii, Fructus Lycii, Ziziphi Spinosae, Herba Epimedii, the Radix Astragali, Rhizoma Polygonati, Fructus Crataegi, Semen Cuscutae are merged, add water saturates to abundant moistening, being heated to seethe with excitement and keeping micro-boils to well-done, filter, decoction like this and filtration, adding water repeats 2 ~ 3 times, finally merge each filtrate after leaving standstill, get supernatant and separately put the Radix Angelicae Sinensis of preservation, Flos Chrysanthemi medicine (filter) liquid merges, the sorbic acid of 0.15 ~ 0.2% is added, through subpackage sterilizing and get final product after concentrated.
Up to now, have no other documents and openly report that employing Different Preparation prepares this medicine.But in the preparation technology that this patent provides all medicines be all adopt water extraction dry again approach to obtain effective ingredient, adopt the Chinese medicine composition that this method for making is obtained, its active constituent content is low, in throughput process, waste of raw materials is large, the pharmaceutical preparations obtained is difficult to drying, and the stability of medicine is low.
Therefore, be badly in need of providing a kind of new technology preparing the anti-ageing Chinese patent medicine of the brute force with anti-aging effects, to provide, curative effect is better, good stability, safe drugs.
Summary of the invention
The object of the present invention is to provide a kind of preparation technology with the anti-ageing Chinese patent medicine of brute force of anti-aging effects, compared with compositions prepared by this technique compositions prepared and the method for making that contrast patent provides, its drug absorption Du Gengjia, bioavailability are higher, better efficacy.
The preparation technology with the anti-ageing Chinese patent medicine of brute force of anti-aging effects provided by the invention, specific as follows:
Prescription:
Fructus Lycii 5 ~ 6 parts of Radix Polygoni Multiflori 6 ~ 7 parts of Fructus Ligustri Lucidi 5 ~ 6 parts of Radix Angelicae Sinensis 5 ~ 7 parts of Semen Cuscutae 10 ~ 12 parts of Herba Epimedii 4 ~ 6 parts of Radixs Astragali 7 ~ 9 parts of Rhizoma Polygonatis 10 ~ 12 parts of Radix Salviae Miltiorrhizaes 5 ~ 6 portions of Fructus Crataegis 8 ~ 9 parts of Semen Ziziphi Spinosaes 5 ~ 6 parts of Flos Chrysanthemis 11 ~ 12 parts of Cortex Lycii 5 ~ 6 parts
Preparation technology:
1) get Radix Polygoni Multiflori sheet, steam about 6 ~ 8 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use.
2) get Radix Angelicae Sinensis 5-7 part and be ground into most coarse powder, use 70-90% ethanol as solvent, dipping 24-48h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, and to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use.Filtering residue collected by another device.
3) get Fructus Lycii 5 ~ 6 parts, Fructus Ligustri Lucidi 5 ~ 6 parts, Semen Cuscutae 10 ~ 12 parts, Herba Epimedii 4 ~ 6 parts, the Radix Astragali 7 ~ 9 parts, Radix Salviae Miltiorrhizae 5 ~ 6 parts, Fructus Crataegi 8 ~ 9 parts, Semen Ziziphi Spinosae 5 ~ 6 parts, Cortex Lycii 5 ~ 6 parts be ground into most coarse powder with 60 ~ 90% ethanol for solvent soaking spends the night, add 6-15 times amount 70-90% alcohol reflux 1-3 time, each 1-3h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device.
4) get Flos Chrysanthemi 11 ~ 12 parts and be ground into most coarse powder, add the water of 6-9 times amount, soak 2-3h, hydrodistillation extracts volatile oil, and extraction time is 3-5h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use.
5) Radix Polygoni Multiflori Preparata 5 ~ 7 parts, Rhizoma Polygonati 10 ~ 12 parts is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3-5h of 6-9 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.15-1.50, drying, adds above-mentioned volatile oil, mixes and get final product.
Said method also comprises to step 5) product that obtains adds appropriate adjuvant, makes various dosage form.Further preferably, described dosage form includes but not limited to granule, tablet, pill, powder, capsule.
Preferably:
1) get Radix Polygoni Multiflori sheet, steam about 6 ~ 8 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use.
2) get Radix Angelicae Sinensis 5-7 part and be ground into most coarse powder, use 80-90% ethanol as solvent, dipping 32-40h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, and to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use.Filtering residue collected by another device.
3) get Fructus Lycii 5 ~ 6 parts, Fructus Ligustri Lucidi 5 ~ 6 parts, Semen Cuscutae 10 ~ 12 parts, Herba Epimedii 4 ~ 6 parts, the Radix Astragali 7 ~ 9 parts, Radix Salviae Miltiorrhizae 5 ~ 6 parts, Fructus Crataegi 8 ~ 9 parts, Semen Ziziphi Spinosae 5 ~ 6 parts, Cortex Lycii 5 ~ 6 parts be ground into most coarse powder with 70 ~ 80% ethanol for solvent soaking spends the night, add 8-12 times amount 75-85% alcohol reflux 2-3 time, each 1.5-2.5h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device.
4) get Flos Chrysanthemi 11 ~ 12 parts and be ground into most coarse powder, add the water of 8-9 times amount, soak 2.5-3h, hydrodistillation extracts volatile oil, and extraction time is 3-4h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use.
5) Radix Polygoni Multiflori Preparata 5 ~ 7 parts, Rhizoma Polygonati 10 ~ 12 parts is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3-4h of 7-9 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.15-1.50, drying, adds above-mentioned volatile oil, mixes and get final product.
Said method also comprises to step 5) product that obtains adds appropriate adjuvant, makes various dosage form.Further preferably, described dosage form includes but not limited to granule, tablet, pill, powder, capsule.
More preferably:
1) get Radix Polygoni Multiflori sheet 180g, steam about 7 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use.
2) get Radix Angelicae Sinensis 240g part and be ground into most coarse powder, use 80% ethanol as solvent, dipping 36h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, and to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use.Filtering residue collected by another device.
3) get Fructus Lycii 180g, Fructus Ligustri Lucidi 180g, Semen Cuscutae 360g, Herba Epimedii 180g, Radix Astragali 270g, Radix Salviae Miltiorrhizae 180g, Fructus Crataegi 270g, Semen Ziziphi Spinosae 180g, Cortex Lycii 180g be ground into most coarse powder with 80% ethanol for solvent soaking spends the night, add 12 times amount 80% alcohol reflux 3 times, each 2h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device.
4) get Flos Chrysanthemi 360g part and be ground into most coarse powder, add the water of 9 times amount, soak 3h, hydrodistillation extracts volatile oil, and extraction time is 3h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use.
5) Radix Polygoni Multiflori Preparata 180g, Rhizoma Polygonati 360g part is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3h of 8 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.20, drying, adds above-mentioned volatile oil, mixes and get final product.
More preferably, said method also comprises to step 5) products therefrom adds appropriate adjuvant, makes various dosage form.Further preferably, described dosage form includes but not limited to granule, tablet, pill, powder, capsule.
Such as: by obtained pharmaceutical preparations, add dried cream powder 10 parts, sucrose 10 parts, 5 parts, dextrin, add appropriate 70% ethanol and make granule, dry, to obtain final product.
Such as: by obtained pharmaceutical preparations, add dried cream powder 10 parts, sucrose 10 parts, 5 parts, dextrin, add appropriate 70% ethanol and make granule, dry, granulate tabletting, film coating or sugar-coat, to obtain final product.
Such as: by obtained pharmaceutical preparations, every 10 parts of fine powders add refined honey 10 parts and make concentrated honeyed pill, to obtain final product.
Such as: by obtained pharmaceutical preparations, grinding, mixing, packs and get final product.
Such as: by obtained pharmaceutical preparations, add starch 10 parts, magnesium stearate 10 parts, add 70% appropriate amount of ethanol and make granule, dry, granulate, encapsulated and get final product.
The preparation technology that preparation technology provided by the present invention and patent (application number is 92115108.X) provide has significant difference, specific as follows:
1. the present invention adopts Radix Polygoni Multiflori Preparata, there are some researches show, Radix Polygoni Multiflori is after concocting, and effective ingredient changes; Radix Polygoni Multiflori mainly contains removing toxic substances, eliminating carbuncle, preventing the attack (or recurrence) of malaria, loosening bowel to relieve constipation, Radix Polygoni Multiflori Preparata invigorating the liver and kidney, benefiting essence-blood, black beard and hair, and bone and muscle strengthening, changes the effects such as turbid blood fat reducing, and therefore, the present invention selects Radix Polygoni Multiflori Preparata.
2. Radix Angelicae Sinensis decocting in water from former preparation technology extracts and has made independent seepage pressure effects into, pass through By consulting literatures, usually the extraction process adopted for medical material Radix Angelicae Sinensis is water distillation or alcohol reflux, the present invention adopts seepage pressure effects, percolation technological operation is relatively simple, and compared with extracting Radix Angelicae Sinensis with traditional boiled process, its active constituent content of the product that seepage pressure effects Radix Angelicae Sinensis obtains is higher; Ethanol used herein can be recycled simultaneously, decreases production cost.
2. Fructus Lycii, Fructus Ligustri Lucidi, Semen Cuscutae, Herba Epimedii, the Radix Astragali, Radix Salviae Miltiorrhizae, Fructus Crataegi, Semen Ziziphi Spinosae, Cortex Lycii in prescription, has made alcohol reflux into by decocting in water in former preparation technology, and its active constituent content of product that the above-mentioned Chinese medicine of alcohol reflux obtains is higher; Ethanol used herein can be recycled simultaneously, decreases production cost.
3. prescription ZHONGJUHUA has made water distillation into by former preparation technology's decocting in water, remains volatile oil component in Flos Chrysanthemi, and after distilling, medicinal residues have also been obtained and make full use of simultaneously.
Specific embodiment mode
Below in conjunction with specific embodiment, the invention will be further described; and not to the restriction of invention; according to prior art well known in the art; embodiments of the present invention are not limited to this; therefore the equivalent replacement of all this areas made according to present disclosure, all belongs to protection scope of the present invention.
Embodiment 1: different method for making prepares pharmaceutical preparations
Prescription
Fructus Lycii 180g Radix Polygoni Multiflori 180g Fructus Ligustri Lucidi 180g Radix Angelicae Sinensis 240g Semen Cuscutae 360g Herba Epimedii 180g Radix Astragali 270g Rhizoma Polygonati 360g Radix Salviae Miltiorrhizae 180g Fructus Crataegi 270g Semen Ziziphi Spinosae 180g Flos Chrysanthemi 360g Cortex Lycii 180g
1, method for making provided by the invention is adopted to prepare pharmaceutical preparations
Preparation technology:
1) get Radix Polygoni Multiflori sheet 180g, steam about 7 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use.
2) get Radix Angelicae Sinensis 240g part and be ground into most coarse powder, use 80% ethanol as solvent, dipping 36h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, and to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use.Filtering residue collected by another device.
3) get Fructus Lycii 180g, Fructus Ligustri Lucidi 180g, Semen Cuscutae 360g, Herba Epimedii 180g, Radix Astragali 270g, Radix Salviae Miltiorrhizae 180g, Fructus Crataegi 270g, Semen Ziziphi Spinosae 180g, Cortex Lycii 180g be ground into most coarse powder with 80% ethanol for solvent soaking spends the night, add 12 times amount 80% alcohol reflux 3 times, each 2h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device.
4) get Flos Chrysanthemi 360g part and be ground into most coarse powder, add the water of 9 times amount, soak 3h, hydrodistillation extracts volatile oil, and extraction time is 3h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use.
5) Radix Polygoni Multiflori Preparata 180g, Rhizoma Polygonati 360g part is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3h of 8 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.15-1.50, dry, add above-mentioned volatile oil, mixing, fine powder is made in spray-dried, pulverizing, obtains pharmaceutical preparations A.
2, the preparation technology of contrast patent is adopted to prepare Kang Xin soft capsule
Get Radix Angelicae Sinensis 240g, Flos Chrysanthemi 360g adds water 2500ml immersion about 30 minutes, is heated to boil, and filters after keeping micro-boiling 15 minutes, the another device of filtrate is preserved, by the Radix Angelicae Sinensis of gained after above-mentioned filter, the medicinal residues of Flos Chrysanthemi and Cortex Lycii 180g, Radix Polygoni Multiflori 180g, Radix Salviae Miltiorrhizae 180g, Fructus Ligustri Lucidi 180g, Fructus Lycii 180g, Semen Ziziphi Spinosae 180g, Herba Epimedii 180g, Radix Astragali 270g, Fructus Crataegi 270g, Rhizoma Polygonati 360g, Semen Cuscutae 360g merges, add water 7500ml, soak 30min and make it fully moistening, carrying out first time decocts and is heated to boil and filters after keeping micro-boiling 120 minutes, filtrate is separately deposited, filtering residue adds water 5000ml again, then carry out second time to decoct, be heated to boil and filter after keeping micro-boiling 60 minutes, the filtrate of twice decoction being filtered afterwards merges, supernatant is got after staticly settling 12 hours, be concentrated in right amount, with the Radix Angelicae Sinensis of above-mentioned gained, Flos Chrysanthemi filtrate merges, be heated to boil 15 minutes, add sorbic acid 15g, spray-dried, fine powder is made in pulverizing, obtain pharmaceutical preparations B.
Embodiment 2: the pharmaceutical preparations of Different Preparation gained is on the impact of D-galactose institute Aging mice learning and memory behavior
1, test material: pharmaceutical preparations A, the B of preparation in embodiment 1 of the present invention, content is 0.75g/ bag (every symphysis medicine is about 5.6g).
2, experimental animal: 90 4 monthly age Kunming mouses, male, body weight 20 ± 2g.
3, aging model is set up: except Normal group, and dosage is 140mg/kg × d, every day 1 time, continuous 6 weeks, induces aging model in neck dorsal sc injection 2%D-galactose every day for each group mice; Normal group injecting normal saline 0.1ml/10g.
4, animal grouping: mice is divided into 9 groups at random, i.e. dosage group (1.5g/kg), B low dose group (0.75g/kg) and piracetam group (600mg/kg) in dosage group (1.5g/kg), A low dose group (0.75g/kg), B high dose group (3.0g/kg), B in Normal group, D-galactose model group, A high dose group (3.0g/kg), A
5, administration: start gastric infusion from the modeling of each group, according to the administration of 10ml/kg body weight, model group and Normal group gavage equal-volume normal saline, continuous 6 weeks.Mice is weighed once weekly Monday, according to body weight adjustment dosage.
6. statistical analysis
Experimental result adopts SPSS18.0 statistical software to carry out statistical procedures, and data are used
represent; Compare between many groups, variance uses variance analysis together, heterogeneity of variance non parametric tests, and P < 0.05 has significant difference.
7 autonomic activitieses measure
Test is put into reaction chamber with mice, every lattice one, measure the movable number of times of mice in 5min and number of times of standing, as activity indicators.After 8th day gastric infusion 1h, mice is put into autonomic activities case, first conform 3min, then records the movable number of times in 5min and number of times of standing.
Table 1 is on the impact of D-galactose institute Aging mice autonomic activities
As shown in Table 1, Normal group, D-galactose model group and each administration group autonomic activities no significant difference after administration 1h; Can be obtained by data in table, the movable number of times of mice measured by D-galactose model group and number of times of standing all are less than Normal group, and the success of modeling is to a certain extent described; The movable number of times of mice measured by each administration group and number of times of standing all are greater than D-galactose model group, illustrate that each medicine has certain help to D-galactose institute Aging mice slow movement; Simultaneously under same dosage level, the movable number of times of the measured mice of pharmaceutical preparations A group and number of times of standing all are greater than pharmaceutical preparations B group, illustrate that pharmaceutical preparations A is better than pharmaceutical preparations B to D-galactose institute Aging mice slow movement effect.
The above results shows, adopts the pharmaceutical preparations of two kinds of method for making gained all can improve the movable number of times of D-galactose institute Aging mice and number of times of standing; Adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent simultaneously, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
8. Jumping test
After last administration, 1h carries out diving tower training, mice is put into diving tower instrument first to conform 3min, base copper grid energising 5min (given voltage is 24V), if mice rebound insulated platform (place of safety) is to hide electric shock, being correct response, being subject to electric shock if again jump on copper grid, is wrong reaction.Test after 24h, be placed on insulated platform during test by mice, record mice jumps off incubation period and the interior number of times (errors number) shocked by electricity of 5min of platform first, as Memory result.If mice rests on more than 5min on platform during test, be then designated as 300s its incubation period.
Table 2 is on the impact of D-galactose institute Aging mice diving tower achievement
Note: compare with Normal group, #P < 0.05, ##P < 0.01; Compare with model group,
*p < 0.05,
*p < 0.01,
△represent that the low dose group of pharmaceutical preparations A is compared with the low dose group of pharmaceutical preparations B,
●represent that the middle dosage group of pharmaceutical preparations A is compared with dosage group in pharmaceutical preparations B, P < 0.05.
As shown in Table 2, compared with Normal group, errors number in D-galactose model group mice 5min increases and obviously shortens (P < 0.01) (P < 0.05), the first incubation period of mistake, and modeling success is described; Errors number in each administration group mice 5min comparatively model group reduces (P < 0.05), and incubation period, comparatively model group obviously extended (P < 0.01); Simultaneously under middle dosage level, mice measured by pharmaceutical preparations A amount group first mistake incubation period be all greater than pharmaceutical preparations B group, and there is significant difference (P < 0.05), errors number in 5min is all less than pharmaceutical preparations B group, and has significant difference (P < 0.05); Under low dosage level, the errors number in pharmaceutical preparations A amount group 5min is all less than pharmaceutical preparations B group, and has significant difference (P < 0.05).
The incubation period that the measured mice of pharmaceutical preparations A group is wrong first under same dose level is all greater than pharmaceutical preparations B group, errors number in 5min is all less than pharmaceutical preparations B group, illustrates that pharmaceutical preparations A is better than pharmaceutical preparations B to D-galactose institute Aging mice slow movement effect.
The above results shows, the pharmaceutical preparations of employing two kinds of method for making gained all can reduce the errors number in mice 5min, extends the incubation period of mistake first; Adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent simultaneously, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
9. water maze laboratory
Water maze is made up of the dark double-deck lucite of 80cm × 50cm × 20cm, establishes 4 place's cecums altogether.Animal grouping and medication all identical with step dow n test.45th-47d trains, 48d test data as Memory result.Is placed near terminal in water by mice before each training, allows its climb steps 2 times voluntarily, 45d stays 2 place's cecums, by mice from starting point in the face of water put into by labyrinth wall.Allow mice swim over to terminal from starting point, train 2 times; 46d stays 3 place's cecums, and the method according to 45d trains 2 times; 47d, 48d all stay 4 place's cecums.After experiment starts, mice head is placed on starting point towards labyrinth wall, record mice is swum the time (incubation period) to terminal from starting point and enters the errors number of labyrinth cecum, and in 3min, trip does not go out person by 3min.If mice can not be swum at the appointed time to step, then guided to step, and make it stop 20s at terminal.
Table 3 is on the impact of D-galactose institute Aging mice water maze laboratory
Note: compare with Normal group,
#p < 0.05,
##p < 0.01; Compare with model group,
*p < 0.05,
*p < 0.01,
△represent that the low dose group of pharmaceutical preparations A is compared with the low dose group of pharmaceutical preparations B,
●represent that the middle dosage group of pharmaceutical preparations A is compared with dosage group in pharmaceutical preparations B, P < 0.05,
★represent that the high dose group of pharmaceutical preparations A is compared with the high dose group of pharmaceutical preparations B, P < 0.05.
As shown in Table 3, compared with Normal group, D-galactose model group mice incubation period and the errors number entering labyrinth cecum obviously extend (P < 0.05), and modeling success is described; Each administration group mice incubation period and the errors number entering labyrinth cecum obviously shorten (P < 0.05); Simultaneously under high, medium and low dosage level, measured by pharmaceutical preparations A amount group, mice is all less than pharmaceutical preparations B group incubation period, and has significant difference (P < 0.05); The errors number that the measured mice of pharmaceutical preparations A group enters labyrinth cecum under same dose level is all less than pharmaceutical preparations B group.
The above results, illustrate that pharmaceutical preparations A is better than pharmaceutical preparations B to D-galactose institute Aging mice learning and memory behavior ability, therefore can adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
Embodiment 3: the pharmaceutical preparations of Different Preparation gained is on the impact of D-galactose Aging mice oxidation resistance
1, test material: pharmaceutical preparations A, the B of preparation in embodiment 1 of the present invention, content is 0.75g/ bag (every symphysis medicine is about 5.6g).
2, experimental animal: 90 4 monthly age Kunming mouses, male, body weight 20 ± 2g.
3, aging model is set up: except Normal group, and dosage is 140mg/kg × d, every day 1 time, continuous 6 weeks, induces aging model in neck dorsal sc injection 2%D-galactose every day for each group mice; Normal group injecting normal saline 0.1ml/10g.
4, animal grouping: mice is divided into 9 groups at random, i.e. dosage group (1.5g/kg), B low dose group (0.75g/kg) and piracetam group (600mg/kg) in dosage group (1.5g/kg), A low dose group (0.75g/kg), B high dose group (3.0g/kg), B in Normal group, D-galactose model group, A high dose group (3.0g/kg), A
5, administration: start gastric infusion from the modeling of each group, according to the administration of 10ml/kg body weight, model group and Normal group gavage equal-volume normal saline, continuous 6 weeks.Mice is weighed once weekly Monday, according to body weight adjustment dosage.
6. collection of specimens and preparation
Prepared by serum: after mice behavior detects and terminates, water 24h is prohibited in fasting, and start to draw materials, mice is extractd eyeball and gets blood, blood leaves standstill 30min, the centrifugal 15min of 3000rpm, conventional separation of serum, and it is for subsequent use to get upper serum.
Prepared by brain, liver, spleen and thymic tissue: after sacrifice, dissects and wins brain, spleen, thymus and liver.The brain taken out, spleen, thymus and the liver normal saline flushing of 0.9%, cleans remained on surface blood, weighs after blotting with filter paper.
Prepared by brain tissue homogenate: by gained cerebral tissue, and accurately add 9 times to the ice-cold normal saline of tissue, homogenizer homogenate, makes 10% brain tissue homogenate, gets supernatant for subsequent use after the centrifugal 15min of 3000rpm.
Prepared by liver homogenate: then shredded by liver, accurately weigh, put into glass homogenizer, the normal saline adding 9 times of volumes carries out homogenate.
7. Testing index
1) in mice serum, liver organization, malonaldehyde (MDA) content, total number born (T-SOD) mensuration that is active and glutathion peroxidase (GSH-Px) activity all operate according to the description in related kit.
2) protein content in liver organization measures according to Coomassie Brilliant Blue.
3) computing formula of organ index: organ index (%)=organ weights/Mouse Weight × 100%
8. statistical analysis
Experimental result adopts SPSS18.0 statistical software to carry out statistical procedures, and data are used
represent; Compare between many groups, variance uses variance analysis together, heterogeneity of variance non parametric tests, and P < 0.05 has significant difference.
9. on the impact of mice organs index
Table 4 is on the impact of mice organs index
Note: compare with Normal group,
#p < 0.05,
##p < 0.01; Compare with model group,
*p < 0.05,
*p < 0.01,
●represent that the middle dosage group of pharmaceutical preparations A is compared with dosage group in pharmaceutical preparations B, P < 0.05,
★represent that the high dose group of pharmaceutical preparations A is compared with the high dose group of pharmaceutical preparations B, P < 0.05.
As shown in Table 4, compared with Normal group, D-galactose model group Organs of Mice index all obviously reduces (P < 0.05), shows that atrophy appears in mouse aging brain and each immune organ, and modeling success is described; Except pharmaceutical preparations B low dosage administration group, other administration groups are equivalent to D-galactose model group Organs of Mice index all obviously to be increased (P < 0.05); Simultaneously under height, middle dosage level, measured by pharmaceutical preparations A amount group, Organs of Mice index is all greater than pharmaceutical preparations B group, and has significant difference (P < 0.05).
The above results, illustrate that pharmaceutical preparations A increases each organ index to D-galactose institute Aging mice and is better than pharmaceutical preparations B, therefore can adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
10. on the impact of MDA content and T-SOD, GSH-Px activity in mouse liver tissue
Table 5 is on the impact of MDA content and T-SOD, GSH-Px activity in mouse liver tissue
Note: compare with Normal group,
#p < 0.05,
##p < 0.01; Compare with model group,
*p < 0.05,
*p < 0.01,
●represent that the middle dosage group of pharmaceutical preparations A is compared with dosage group in pharmaceutical preparations B, P < 0.05.
As shown in Table 5, compared with Normal group, in D-galactose model group mouse liver tissue, MDA content obviously increases (P < 0.01), GSH-Px activity and all obviously reduces (P < 0.05), and modeling success is described; In each administration group mouse liver tissue, MDA content and GSH-Px activity all have significant difference (P < 0.05) compared to model group.Simultaneously under middle dosage level, in the measured mouse liver tissue of pharmaceutical preparations A group, MDA content and GSH-Px activity have significant difference (P < 0.05) compared with pharmaceutical preparations B group.
The above results, illustrate that pharmaceutical preparations A reduces MDA content to D-galactose institute Aging mice, improve T-SOD, GSH-Px activity and be better than pharmaceutical preparations B, therefore can adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
11. impacts on MDA content and T-SOD, GSH-Px activity in mice serum
Table 6 is on the impact of MDA content and T-SOD, GSH-Px activity in mice serum
Note: compare with Normal group,
#p < 0.05; Compare with model group,
*p < 0.05,
*p < 0.01.
As shown in Table 6, compared with Normal group, in D-galactose model group mice serum, MDA content and T-SOD, GSH-Px activity all have significant difference (P < 0.05), and modeling success is described; In each administration group mice serum, MDA content all obviously reduces (P < 0.05) relative to D-galactose model group mice; Except pharmaceutical preparations B low dosage administration group, in other administration group mice serums, T-SOD activity obviously increases (P < 0.05) compared to D-galactose model group; In pharmaceutical preparations A group and positive drug group mice serum, GSH-Px is active all higher than D-galactose model group (P < 0.05).
The above results, illustrate that pharmaceutical preparations A is better than pharmaceutical preparations B to MDA content, increase T-SOD and GSH-Px activity in reduction D-galactose institute Aging mice serum, therefore can adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more remarkable effect for the preparation technology's gained pharmaceutical preparations B described in employing contrast patent, further illustrate preparation technology provided by the present invention and be better than contrasting the preparation technology described in patent.
Embodiment 4: the pharmaceutical preparations stability test contrast of Different Preparation gained
Influence factor tests: in Example 1, pharmaceutical preparations A, the pharmaceutical preparations B of gained carries out hot test (60 DEG C), high wet test (relative humidity 92.5%, 25 DEG C) and exposure experiments to light (4500lx), respectively at sampling in the 0th, 5,10 day, check according to every test method, and compared with 0 day, the results are shown in following table 7.
The pharmaceutical preparations influence factor result of the test of table 7 Different Preparation gained
Table 7 result shows, and under the high temperature conditions, Radix Angelicae Sinensis dysmenorrhea pharmaceutical preparations A, B occurred the phenomenons such as layering, granularity increase, mastic water outlet respectively at the 10th day, the 5th day, illustrate that pharmaceutical preparations A is more stable under the high temperature conditions than pharmaceutical preparations B; Under conditions of high humidity, there is the phenomenons such as layering, granularity increase, mastic water outlet in DO prepared product B, shows that pharmaceutical preparations A is more stable under conditions of high humidity than pharmaceutical preparations B at the 10th day; Under light illumination, there is the phenomenons such as layering, granularity increase, mastic water outlet in pharmaceutical preparations A, B, illustrates under light illumination, both stability there was no significant differences at the 10th day.In sum, under above-mentioned influence factor's condition, adopt the pharmaceutical preparations A of preparation technology's gained provided by the invention more to stablize than preparation technology's gained pharmaceutical preparations B of employing contrast described in patent and can show that preparation technology provided by the invention is better than contrasting the preparation technology of patent.
In sum, in pharmacodynamics, adopt the present invention the medicine prepared of described production technology, be better than adopting medicine preparation technology prepared by of contrast described in patent; The medicine simultaneously adopting preparation technology of the present invention to prepare is more stable.In sum, preparation technology provided by the present invention can be obtained be better than contrasting the preparation technology described in patent.
Embodiment 5: the preparation of the pharmaceutical preparations granule of the preparation technology's gained adopting this patent to provide
By the pharmaceutical preparations A of gained in embodiment 1, add appropriate dried cream powder, sucrose, dextrin, appropriate amount of ethanol make granule, dry, mix and get final product.
Embodiment 6: the preparation of the pharmaceutical preparations tablet of the preparation technology's gained adopting this patent to provide
By the pharmaceutical preparations A of gained in embodiment 1, add appropriate dried cream powder, sucrose, dextrin, appropriate amount of ethanol make granule, dry, granulate, tabletting, film coating or sugar-coat, to obtain final product.
Embodiment 7: the preparation of the pharmaceutical preparations pill of the preparation technology's gained adopting this patent to provide
By the pharmaceutical preparations A of gained in embodiment 1, add appropriate refined honey and make concentrated honeyed pill, to obtain final product.
Embodiment 8: the preparation of the pharmaceutical preparations powder of the preparation technology's gained adopting this patent to provide
By the pharmaceutical preparations A of gained in embodiment 1, grinding, mixing, packs and get final product.
Embodiment 9: the preparation of the pharmaceutical preparations capsule of the preparation technology's gained adopting this patent to provide
By the pharmaceutical preparations A of gained in embodiment 1, add appropriate amount of starch and magnesium stearate, make granule with the ethanol of debita spissitudo, dry, granulate, encapsulated and get final product.
Claims (10)
1. have a preparation technology for the anti-ageing Chinese patent medicine of brute force of anti-aging effects, described technique is specific as follows:
Prescription:
Fructus Lycii 5 ~ 6 parts of Radix Polygoni Multiflori 6 ~ 7 parts of Fructus Ligustri Lucidi 5 ~ 6 parts of Radix Angelicae Sinensis 5 ~ 7 parts of Semen Cuscutae 10 ~ 12 parts of Herba Epimedii 4 ~ 6 parts of Radixs Astragali 7 ~ 9 parts of Rhizoma Polygonatis 10 ~ 12 parts of Radix Salviae Miltiorrhizaes 5 ~ 6 portions of Fructus Crataegis 8 ~ 9 parts of Semen Ziziphi Spinosaes 5 ~ 6 parts of Flos Chrysanthemis 11 ~ 12 parts of Cortex Lycii 5 ~ 6 parts
Preparation technology:
1) get Radix Polygoni Multiflori sheet, steam about 6 ~ 8 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use;
2) get Radix Angelicae Sinensis 5-7 part and be ground into most coarse powder, use 70-90% ethanol as solvent, dipping 24-48h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use, and filtering residue collected by another device;
3) get Fructus Lycii 5 ~ 6 parts, to be ground into most coarse powder be that solvent soaking spends the night with 60 ~ 90% ethanol for Fructus Ligustri Lucidi 5 ~ 6 parts, Semen Cuscutae 10 ~ 12 parts, Herba Epimedii 4 ~ 6 parts, the Radix Astragali 7 ~ 9 parts, Radix Salviae Miltiorrhizae 5 ~ 6 parts, Fructus Crataegi 8 ~ 9 parts, Semen Ziziphi Spinosae 5 ~ 6 parts, Cortex Lycii 5 ~ 6 parts, add 6-15 times amount 70-90% alcohol reflux 1-3 time, each 1-3h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device;
4) get Flos Chrysanthemi 11 ~ 12 parts and be ground into most coarse powder, add the water of 6-9 times amount, soak 2-3h, hydrodistillation extracts volatile oil, and extraction time is 3-5h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use;
5) Radix Polygoni Multiflori Preparata 5 ~ 7 parts, Rhizoma Polygonati 10 ~ 12 parts is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3-5h of 6-9 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.15-1.50, drying, adds above-mentioned volatile oil, mixes and get final product.
2. there is the preparation technology of the anti-ageing Chinese patent medicine of brute force of anti-aging effects according to claim 1, it is characterized in that:
1) get Radix Polygoni Multiflori sheet, steam about 6 ~ 8 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use;
2) get Radix Angelicae Sinensis 5-7 part and be ground into most coarse powder, use 80-90% ethanol as solvent, dipping 32-40h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use, and filtering residue collected by another device;
3) get Fructus Lycii 5 ~ 6 parts, to be ground into most coarse powder be that solvent soaking spends the night with 70 ~ 80% ethanol for Fructus Ligustri Lucidi 5 ~ 6 parts, Semen Cuscutae 10 ~ 12 parts, Herba Epimedii 4 ~ 6 parts, the Radix Astragali 7 ~ 9 parts, Radix Salviae Miltiorrhizae 5 ~ 6 parts, Fructus Crataegi 8 ~ 9 parts, Semen Ziziphi Spinosae 5 ~ 6 parts, Cortex Lycii 5 ~ 6 parts, add 8-12 times amount 75-85% alcohol reflux 2-3 time, each 1.5-2.5h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device;
4) get Flos Chrysanthemi 11 ~ 12 parts and be ground into most coarse powder, add the water of 8-9 times amount, soak 2.5-3h, hydrodistillation extracts volatile oil, and extraction time is 3-4h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use;
5) Radix Polygoni Multiflori Preparata 5 ~ 7 parts, Rhizoma Polygonati 10 ~ 12 parts is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3-4h of 7-9 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.15-1.50, drying, adds above-mentioned volatile oil, mixes and get final product.
3. there is the preparation technology of the anti-ageing Chinese patent medicine of brute force of anti-aging effects according to claim 1-2, it is characterized in that:
1) get Radix Polygoni Multiflori sheet 180g, steam about 7 hours, stewing 12 hours, take out; Dry to 8 one-tenths and do, mix thoroughly with decoction of black soybean, after juice exhausts, then steam about 24 hours, black dry and get final product to interior for spending, for subsequent use;
2) get Radix Angelicae Sinensis 240g part and be ground into most coarse powder, use 80% ethanol as solvent, dipping 36h, slowly percolation, collect liquid of just filtering, another device is preserved, and continues percolation, and to percolate closely colourless or micro-yellow, collect continuous liquid of filtering, merging filtrate is for subsequent use, and filtering residue collected by another device;
3) get Fructus Lycii 180g, Fructus Ligustri Lucidi 180g, Semen Cuscutae 360g, Herba Epimedii 180g, Radix Astragali 270g, Radix Salviae Miltiorrhizae 180g, Fructus Crataegi 270g, Semen Ziziphi Spinosae 180g, Cortex Lycii 180g be ground into most coarse powder with 80% ethanol for solvent soaking spends the night, add 12 times amount 80% alcohol reflux 3 times, each 2h, filter, merging filtrate, decompression recycling ethanol, collection filtrate is for subsequent use, and filtering residue collected by another device;
4) get Flos Chrysanthemi 11 kg part and be ground into most coarse powder, add the water of 9 times amount, soak 3h, hydrodistillation extracts volatile oil, and extraction time is 3h, collects volatile oil, and distillation rear solution is collected for subsequent use, and the another device of medicinal residues is collected for subsequent use;
5) Radix Polygoni Multiflori Preparata 180g, Rhizoma Polygonati 360g part is got, with above-mentioned 2), 3), 4) gained medicinal residues add the soak by water 3h of 8 times amount in step, filter, filtrate and above-mentioned 2), 3), 4) gained solution merges in step, being evaporated to relative density at 70 DEG C is 1.20, drying, adds above-mentioned volatile oil, mixes and get final product.
4. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-3, it is characterized in that, the pharmaceutical preparations that described technique also comprises to obtaining adds appropriate amount of auxiliary materials, makes various dosage form.
5. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-4, is characterized in that, described technique also comprises and adds appropriate dried cream powder to the pharmaceutical preparations obtained, sucrose, dextrin, add appropriate 70% ethanol again, dry, make granule.
6. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-4, it is characterized in that, described technique also comprises the pharmaceutical preparations to obtaining, and adds appropriate dried cream powder, sucrose, dextrin, add appropriate 70% ethanol again and make granule, dry, granulate tabletting, film coating or sugar-coat, make tablet.
7. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-4, it is characterized in that, described technique also comprises to obtained pharmaceutical preparations, adds appropriate refined honey, concentrates and makes pill.
8. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-4, is characterized in that, described technique also comprises obtained pharmaceutical preparations, grinding, mixing, and packaging makes powder.
9. the preparation technology of the anti-ageing Chinese patent medicine of the brute force with anti-aging effects according to claim 1-4, it is characterized in that, described technique also comprises to obtained pharmaceutical preparations, add appropriate amount of starch, magnesium stearate, then add appropriate 70% ethanol and make granule, dry, granulate, encapsulatedly makes capsule.
10. the preparation technology with the anti-ageing Chinese patent medicine of brute force of anti-aging effects according to claim 1, it is characterized in that, described dosage form includes but not limited to granule, tablet, pill, powder and capsule.
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| CN1088447A (en) * | 1992-12-22 | 1994-06-29 | 福建中医学院 | A kind of powerful anti-ageing Chinese patent medicine with anti-aging effects |
| CN1509737A (en) * | 2002-12-23 | 2004-07-07 | 江苏康缘药业股份有限公司 | Chinese medicinal composition with delaying senility and improving sleep functions, and preparing method thereof |
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| CN1088447A (en) * | 1992-12-22 | 1994-06-29 | 福建中医学院 | A kind of powerful anti-ageing Chinese patent medicine with anti-aging effects |
| CN1509737A (en) * | 2002-12-23 | 2004-07-07 | 江苏康缘药业股份有限公司 | Chinese medicinal composition with delaying senility and improving sleep functions, and preparing method thereof |
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