CN104046510A - Method for preparing collagen peptide from scaly fish skin - Google Patents
Method for preparing collagen peptide from scaly fish skin Download PDFInfo
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- CN104046510A CN104046510A CN201310083632.4A CN201310083632A CN104046510A CN 104046510 A CN104046510 A CN 104046510A CN 201310083632 A CN201310083632 A CN 201310083632A CN 104046510 A CN104046510 A CN 104046510A
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- skin
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- squama fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention relates to a method for preparing collagen peptide from scaly fish skin. The invention especially relates to a method for using fresh or dried scaly fish skin as a raw material, recovering crude fish oil and crude hydroxyapatite therefrom and preparing high-purity collagen peptide.
Description
Technical field
The present invention relates to a kind of from preparing the method for collagen peptide squama fish-skin.Present invention is specifically related to and a kind ofly take fresh or to dry band squama fish-skin be raw material, therefrom reclaim crude fish oil, thick hydroxyapatite and prepare the method for high purity collagen peptide.
Technical background:
Collagen peptide is a kind of biologically active substance with multiple physiological effect, is widely used in recent years the industry fields such as food, healthcare products and makeup, and market demand increases year by year, constantly expands.
China is more about preparing document and the patent of collagen peptide, but is limited to production technology, is raw material at present mainly with deliming fish scale or fish-skin.As ZL200510044916.8, use acid system deliming enzymolysis again, except causing the loss of hydroxyapatite resource, chemical treatment has produced severe contamination to environment, and desquamation simultaneously, the complicated process that strong acid soaks, cleans repeatedly have also increased raw materials cost.ZL200410024145.1 need be used and clean fish-skin is raw material, and lipid resource is not recycled utilization.
In band squama fish-skin solid substance, containing organism 56%-73%, comprise fish oil, collagen protein, Yelkin TTS etc., inanimate matter 25%-33%, take hydroxyapatite as main.Fresh or what dry is that desirable collagen peptide processes raw material with squama fish-skin.
Summary of the invention
The object of the invention is to make full use of fresh or dry band squama fish-skin resource, a kind of method that reclaims crude fish oil, thick hydroxyapatite and prepare high purity collagen peptide is provided.
The present invention be take fresh or to dry band squama fish-skin be raw material, and step is as follows:
1. take CO2 as extraction agent, utilize supercritical technology, complete from reclaiming the process of grease deodorization squama fish-skin, operational condition is 40 ~ 80 ℃, pressure 8 ~ 40MPa.
2. the band squama fish-skin obtaining is crushed to 40 ~ 80 orders, rear use extruding and puffing technology is processed, and in process, using pH is that 9 ~ 10 water is in harmonious proportion, and controlling total Water is 15 ~ 20%, and temperature is 120 ~ 180 ℃, and operating pressure is 0.5 ~ 1 MPa, and the expanded time is 10 ~ 40 seconds.
3. gained puff is used papain enzymolysis, and enzyme dosage is with 1 ~ 2% of squama fish-skin raw material, and condition is pH 6.0 ~ 8.0, and the water yield is 5 ~ 10 times with squama fish-skin raw material, 50 ~ 60 ℃ of temperature, 2 ~ 5 hours time.
4. enzymolysis solution is separated, and residue obtained be thick hydroxyapatite, gained supernatant liquor purified after dry high purity collagen peptide, the purifying of supernatant liquor can be used filtrate Plate Filtration or membrane filtration, dryly can use that spraying is dried etc.
The present invention has the following advantages:
1, the present invention breaks through existing collagen peptide production technology and can only take the limitation that deliming fish scale or fish-skin be raw material, when promoting the level of resources utilization, has avoided fish-skin/fish scale is separated and fish scale deliming produces cost and problem of environmental pollution.
2, this technique gained collagen peptide free from extraneous odour, without bitter taste, molecular weight is concentrated, purity is high.
Embodiment 1:
Fresh band squama fish-skin is put into extractor, and control condition is 80 ℃, and pressure 40MPa, with CO
2for extraction agent, complete supercritical extraction, now grease and small-molecule substance obtain hair fish oil after discharging with extraction agent.Remaining band squama fish-skin is crushed to 80 orders, and adding 15%pH is that 9 ~ 10 water is in harmonious proportion, and uses extruding puffing, and controlling temperature is 180 ℃, and operating pressure is 0.5 MPa, and the expanded time is 20 seconds.Gained puff is used papain enzymolysis, and enzyme dosage is with 1.5% of squama fish-skin raw material, and condition is pH 7.0, and the water yield is 8 times with squama fish-skin raw material, 50 ~ 60 ℃ of temperature, 4 hours time.Enzymolysis solution is separated, and residue obtained be thick hydroxyapatite, and gained supernatant liquor is spray-dried after ultrafiltration membrance filter purifying must high purity collagen peptide, and now the purity of collagen peptide is 98%, and molecular weight 1000Dal accounts for the more than 90% of total protein concentration.
Embodiment 2:
Fresh band squama fish-skin is put into extractor, and control condition is 60 ℃, and pressure 35MPa, with CO
2for extraction agent, complete supercritical extraction, now grease and small-molecule substance obtain hair fish oil after discharging with extraction agent.Remaining band squama fish-skin is crushed to 60 orders, and adding 18%pH is that 9 ~ 10 water is in harmonious proportion, and uses extruding puffing, and controlling temperature is 160 ℃, and operating pressure is 0.8 MPa, and the expanded time is 30 seconds.Gained puff is used papain enzymolysis, and enzyme dosage is with 1% of squama fish-skin raw material, and condition is pH 8.0, and the water yield is 6 times with squama fish-skin raw material, 50 ~ 60 ℃ of temperature, time 2 h.Enzymolysis solution is separated, and residue obtained be thick hydroxyapatite, and gained supernatant liquor is spray-dried after micro-filtrate membrane filtration purifying must high purity collagen peptide, and now the purity of collagen peptide is 92%, and molecular weight 1000Dal accounts for the more than 85% of total protein concentration.
Embodiment 3:
To dry band squama fish-skin and put into extractor, control condition is 40 ℃, and pressure 20MPa, with CO
2for extraction agent, complete supercritical extraction, now grease and small-molecule substance obtain hair fish oil after discharging with extraction agent.Remaining band squama fish-skin is crushed to 40 orders, and adding 20%pH is that 9 ~ 10 water is in harmonious proportion, and uses extruding puffing, and controlling temperature is 120 ℃, and operating pressure is 1 MPa, and the expanded time is 40 seconds.Gained puff is used papain enzymolysis, and enzyme dosage is with 2% of squama fish-skin raw material, and condition is pH 6.0, and the water yield is 10 times with squama fish-skin raw material, 50 ~ 60 ℃ of temperature, 5 hours time.Enzymolysis solution is separated, and residue obtained be thick hydroxyapatite, and gained supernatant liquor is spray-dried after carclazyte filtrate Plate Filtration must high purity collagen peptide, and now the purity of collagen peptide is 88%, and molecular weight 1000Dal accounts for 90% of total protein concentration.
Claims (7)
1. the present invention relates to a kind ofly from reclaiming crude fish oil, thick hydroxyapatite squama fish-skin and preparing the method for high purity collagen peptide, step is as follows: a utilizes supercritical technology, completes from reclaiming the process of grease deodorization squama fish-skin; B is used puffing technique to process on the band squama fish-skin obtaining after crushed; C gained puff enzymolysis; D is separated by enzymolysis solution, and residue obtained be thick hydroxyapatite, and gained supernatant liquor is purified must high purity collagen peptide after dry.
2. method according to claim 1, is characterized in that: selected can be fresh with squama fish-skin raw material, also can dry.
3. method according to claim 1, is characterized in that: a step supercritical technology used is with CO
2for extraction agent, operational condition is 40 ~ 80 ℃, pressure 8 ~ 40MPa.
4. method according to claim 1, is characterized in that: in b step, grinding particle size will reach 40 ~ 80 orders.
5. method according to claim 1, is characterized in that: in b step, use extruding puffing, in process, using pH is that 9 ~ 10 water is in harmonious proportion, and controlling total Water is 15 ~ 20%, and temperature is 120 ~ 180 ℃, and operating pressure is 0.5 ~ 1 MPa, and the expanded time is 10 ~ 40 seconds.
6. method according to claim 1, is characterized in that: in c step, use papoid, enzyme dosage is with 1 ~ 2% of squama fish-skin raw material, and condition is pH 6.0 ~ 8.0, and the water yield is 5 ~ 10 times with squama fish-skin raw material, 50 ~ 60 ℃ of temperature, 2 ~ 5 hours time.
7. method according to claim 1, is characterized in that: in d step, the purifying of enzymolysis supernatant liquor can be used filtrate Plate Filtration or membrane filtration, dry can use, spray dry etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310083632.4A CN104046510A (en) | 2013-03-17 | 2013-03-17 | Method for preparing collagen peptide from scaly fish skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310083632.4A CN104046510A (en) | 2013-03-17 | 2013-03-17 | Method for preparing collagen peptide from scaly fish skin |
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| CN104046510A true CN104046510A (en) | 2014-09-17 |
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| CN201310083632.4A Pending CN104046510A (en) | 2013-03-17 | 2013-03-17 | Method for preparing collagen peptide from scaly fish skin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170120A (en) * | 2018-06-08 | 2019-01-11 | 赵雅洲 | The method and Cornu Cervi bulking machine of extruding enzymatic isolation method extraction Cornu Cervi collagen |
| CN111793145A (en) * | 2020-07-02 | 2020-10-20 | 湖南伍星生物科技有限公司 | Process for improving quality and yield of sodium chondroitin sulfate co-produced collagen peptide |
| CN114574538A (en) * | 2022-03-29 | 2022-06-03 | 吴家麟 | Production method for preparing gelatin for capsules by using pigskin enzyme method |
Citations (6)
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| CN1683550A (en) * | 2005-03-08 | 2005-10-19 | 国家海洋局第一海洋研究所 | Collagen and its preparing process |
| CN101376868A (en) * | 2008-09-29 | 2009-03-04 | 泰祥集团技术开发有限公司 | Method for preparing fish oil ethyl ester from fish wastes |
| CN101715838A (en) * | 2009-11-26 | 2010-06-02 | 上海海洋大学 | Method for preparing tuna oil |
| CN101724677A (en) * | 2009-12-15 | 2010-06-09 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion |
| CN101736066A (en) * | 2010-01-08 | 2010-06-16 | 武汉普赛特膜技术循环利用有限公司 | Method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin |
| CN102212597A (en) * | 2011-03-23 | 2011-10-12 | 李世泰 | Method for producing collagen polypeptide, natural pigment and fish oil by using fish skin and fish scale |
-
2013
- 2013-03-17 CN CN201310083632.4A patent/CN104046510A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683550A (en) * | 2005-03-08 | 2005-10-19 | 国家海洋局第一海洋研究所 | Collagen and its preparing process |
| CN101376868A (en) * | 2008-09-29 | 2009-03-04 | 泰祥集团技术开发有限公司 | Method for preparing fish oil ethyl ester from fish wastes |
| CN101715838A (en) * | 2009-11-26 | 2010-06-02 | 上海海洋大学 | Method for preparing tuna oil |
| CN101724677A (en) * | 2009-12-15 | 2010-06-09 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion |
| CN101736066A (en) * | 2010-01-08 | 2010-06-16 | 武汉普赛特膜技术循环利用有限公司 | Method for producing no-fishy smell scale collagen peptide by utilizing macroporous absorbent resin |
| CN102212597A (en) * | 2011-03-23 | 2011-10-12 | 李世泰 | Method for producing collagen polypeptide, natural pigment and fish oil by using fish skin and fish scale |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170120A (en) * | 2018-06-08 | 2019-01-11 | 赵雅洲 | The method and Cornu Cervi bulking machine of extruding enzymatic isolation method extraction Cornu Cervi collagen |
| CN111793145A (en) * | 2020-07-02 | 2020-10-20 | 湖南伍星生物科技有限公司 | Process for improving quality and yield of sodium chondroitin sulfate co-produced collagen peptide |
| CN114574538A (en) * | 2022-03-29 | 2022-06-03 | 吴家麟 | Production method for preparing gelatin for capsules by using pigskin enzyme method |
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Application publication date: 20140917 |