CN104031851A - Candida tropicalis Y2 and applications thereof - Google Patents
Candida tropicalis Y2 and applications thereof Download PDFInfo
- Publication number
- CN104031851A CN104031851A CN201410072606.6A CN201410072606A CN104031851A CN 104031851 A CN104031851 A CN 104031851A CN 201410072606 A CN201410072606 A CN 201410072606A CN 104031851 A CN104031851 A CN 104031851A
- Authority
- CN
- China
- Prior art keywords
- candida tropicalis
- waste
- candida
- tropicalis
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
Candida tropicalis Y2 and applications thereof are disclosed. The candida tropicalis Y2 is deposited in the China Center for Type Culture Collection (CCTCC) with the address being the Wuhan university, Wuhan, China on 29 December, 2013, and has an accession number of CCTCC No:M2013726. The candida tropicalis Y2 is rich in nutrition, and can be used for production of aquatic livestock functional bait additives. In addition, mariculture waste or waste water can be adopted as the main nutrient source, and when 0-0.5% by mass volume is added as a supplementary carbon source, the candida tropicalis Y2 can grow rapidly in the mariculture waste or waste water. A cultivation waste fermentation product can be adopted as feed for saprophagous or filter aquatic livestock. A cultivation wastewater fermentation product can be adopted as a feed additive for aquatic livestock.
Description
Technical field:
The invention belongs to technical field of microbe application, be specifically related to a kind of Candida tropicalis Y2 and application thereof.
Background technology:
Yeast nutrition enriches, is of moderate size, a lot of biological good natural baits, yeast is also a class saprophytic microorganism simultaneously, can decompose many kinds of substance, utilize the livestock wastes such as organism in aquaculture water, ammonia nitrogen, hydrogen sulfide, and form dominant population, and effectively suppress other harmful microbe growth, play the effect of biological control.Along with the progress of aquaculture technology and going deep into that aquatic animal nutritional needs is understood, the application of yeast in aquaculture is more and more extensive in recent years.
Yet the yeast that can be applicable in the market sea farming is also considerably less, conventional yeast remains the bread yeast (Saccharomyces cerevisiae) that comes from food brewing, this yeast that comes from terrestial enviornment can not adapt to briny environment, therefore can not bring into play the effect of purifying sea water water quality, its impact for marine fishery growth of animal also lacks the report of science.Based on this kind of situation, the unicellular yeast that is adapted to ocean environment in the urgent need to separation is for marine aquaculture.On the other hand, in the large scale culturing of yeast, conventionally need some agricultural byproducts as raw material, increased production cost, therefore need to explore new alternative nutrition source, to save cost.
In addition, the scale of China's sea farming in the last thirty years and output constantly increase, and occupy first place in the world at present, yet have also brought serious problem of environmental pollution fast-developing time.Along with the raising of people's environmental consciousness, government implements strict gradually control for the emissions of aquaculture, so the friendly type aquaculture of development environment pattern, and walking continuable aquaculture road for development becomes inexorable trend.At present, although some breeding wastewater treatment processs are separated livestock waste from water body, can not effectively utilize livestock waste.The recycling that how to carry out breeding wastewater, refuse is the problem that the friendly type aquaculture pattern of development environment must solve.
Summary of the invention:
First object of the present invention is to provide a kind ofly to be had high nutritive value, efficiently utilize sea farming refuse, waste water and can improve candida tropicalis (Candida tropicalis) Y2 of fermentation waste local flavor, this bacterium is preserved in Chinese Typical Representative culture collection center (CCTCC) on December 29th, 2013, address: Wuhan, China city Wuhan University, deposit number: CCTCC No:M2013726.
Candida tropicalis of the present invention (Candida tropicalis) Y2 is located away from ShenZhen,GuangDong gold butterfish farm pollution discharge mouth.Yeast isolation substratum is YPD yeast special culture media.Candida tropicalis (Candida tropicalis) Y2 screening culture medium is prawn culturing waste media.The object of this screening is that acquisition can be at the yeast specie of prawn culturing waste media Fast Growth, and separated yeast will have the ability of utilizing livestock waste under low sugar or sugar-free condition.
Colony morphology characteristic and the cell morphological characteristic of Candida tropicalis (Candida tropicalis) Y2: bacterium colony is cultivated 24 hours for white on YPD culture medium flat plate, after 72 hours, be white, the circular projection of bacterium colony, dry, smooth surface or part have wrinkle, tarnish, easily picking, there is wine flavour, cell is spherical to oval, and single or multiple cells are connected, and see sprout (as shown in Figure 1).
Candida tropicalis (Candida tropicalis) Y2 in prawn culturing waste media growth conditions and YPD substratum similar.
With Yeast genome, extract test kit (purchased from Takara, China) and extract DNA.Pcr amplification the 18S rDNA fragment that checks order, its 18S rDNA sequence is as shown in SEQ ID NO.1,18S rDNA sequence is compared by the BLAST software in GeneBank and the sequence in GeneBank database, the 18S rDNA sequence of Candida tropicalis (Candida tropicalis) Y2 bacterial strain and the 18S rDNA sequence relation of a plurality of Candida tropicalis strains are nearest, similarity reaches 100%, and comparison score is also very high.The stammbaum that 18S rDNA sequence based on Candida tropicalis (Candida tropicalis) Y2 bacterial strain and similar sequences thereof build also shows, 18S rDNA sequence and Candida tropicalis (JQ008834) relation of Candida tropicalis (Candida tropicalis) Y2 bacterial strain are nearest, 92% the supporting rate of take is gathered as one, confirm that this yeast is the new bacterial strain of Candida tropicalis, called after candida tropicalis (Candida tropicalis) Y2, this bacterial classification has been preserved in Chinese Typical Representative culture collection center (CCTCC) on December 29th, 2013, address: Wuhan, China city Wuhan University, deposit number: CCTCC No:M2013726.
Through measuring Candida tropicalis of the present invention (Candida tropicalis), Y2 has abundant nutrition, therefore, second object of the present invention is to provide the application of Candida tropicalis (Candida tropicalis) Y2 in the functional additive of bait of producing aquatic animal.
Through the test of fermentation livestock waste, the in the situation that of adding 2.5 ‰ sucrose in livestock waste, to cultivate after Candida tropicalis of the present invention (Candida tropicalis) Y248h, biomass reaches 4.05 * 108cfu/g refuse.When but sucrose concentration is too high, cause on the contrary the biomass of candida tropicalis (Candida tropicalis) Y2 to decline.This sugared concentration of having relatively high expectations from other yeast growth is obviously different.When candida tropicalis (Candida tropicalis) Y2 separated, find that it can not add Fast Growth on the prawn culturing waste media flat board in any sugared source, and other yeast is not grown or poor growth.This result shows, candida tropicalis (Candida tropicalis) Y2 requires lowly to sugar, is particularly suitable for aquaculture refuse that sugar lacks relatively and the disposal of resources of waste water.
Therefore, the 3rd object of the present invention is to provide the application of candida tropicalis (Candida tropicalis) Y2 in sea farming changing waste into resources is disposed.
The application of described candida tropicalis (Candida tropicalis) Y2 in sea farming changing waste into resources is disposed, concrete grammar is: first Candida tropicalis Y2 is incubated in YPD substratum, forms seed liquor.Every 1 kilogram of livestock waste adds 5～10 kilograms of sterilization seawater, by seed liquor with 2～5%(V/W) ratio add wherein, and supplement 0～5 ‰ sucrose as carbon source according to mass volume ratio.Room temperature was cultivated through 24～48 hours, then natural subsidence, abandoned supernatant, was air-dryly sea farming refuse tunning, thereby reached livestock waste higher value application object.After fermentative processing, candida tropicalis (Candida tropicalis) Y2 amount reproduction, utilizes the protein of livestock waste accumulation self, so has improved the value of again utilizing as nutrition of livestock waste; Simultaneously, sea farming refuse tunning presents obvious fermenting aroma, can play the local flavor when improving livestock waste recycling and making bait, improve animal ingestion desire, sea farming refuse tunning can directly be eaten the bait of economic animal as the saprophages such as sea cucumber, shellfish and filter.
The 4th object of the present invention is to provide the application of candida tropicalis (Candida tropicalis) Y2 in marine culture wastewater disposal of resources.
The application of described candida tropicalis (Candida tropicalis) Y2 in marine culture wastewater disposal of resources, concrete grammar is: first Candida tropicalis (Candida tropicalis) Y2 is incubated in YPD substratum, forms seed liquor.By seed liquor with 2～5%(V/V) ratio add in sterile-processed marine culture wastewater.According to mass volume ratio, add 0～5 ‰ sucrose as carbon source, fully stir and mix, room temperature was cultivated through 24～48 hours, after fermentation ends, stir Chi Shui, with water pump, extract respectively Chi Shui out and by hoop, have 10 orders respectively, 20 orders, the cylinder mould of 40 mesh sieve thin,tough silk, water body after filtration was through natural subsidence 4 hours, the white lotion that obtains thickness is marine culture wastewater tunning, the marine culture wastewater tunning obtaining can be directly used in the aquatic animal of throwing something and feeding, also after can being further dried, be packaged into merchandise sales, thereby reach breeding wastewater higher value application object.
Candida tropicalis of the present invention (Candida tropicalis) Y2 is preserved in Chinese Typical Representative culture collection center (CCTCC), address on December 29th, 2013: Wuhan, China city Wuhan University, deposit number: CCTCC No:M2013726.
accompanying drawing explanation:
Fig. 1 is that Candida tropicalis (Candida tropicalis) Y2 cultivates and cellular form figure.A wherein: the colonial morphology of Candida tropicalis (Candida tropicalis) Y2 on YPD flat board; B: Candida tropicalis (Candida tropicalis) Y2 cellular form, the spherical or elliposoidal of cell, gemmation, scale=10 μ m; C: Candida tropicalis (Candida tropicalis) Y2 bacterium liquid is white in color; D: the colonial morphology of Candida tropicalis (Candida tropicalis) Y2 bacterial strain on prawn culturing refuse flat board.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.Method used and technology are conventional method and technology if no special instructions.
Embodiment 1:
One, separation, screening and the evaluation of Candida tropicalis (Candida tropicalis) Y2
(1), separation, the screening of Candida tropicalis (Candida tropicalis) Y2
Get the ShenZhen,GuangDong gold butterfish water port mud 1g of plant, add sterilizing seawater 10ml, after mixing, get 200 μ L coating yeast isolation substratum (YPD) flat boards, YPD is yeast special culture media, preparation method is: glucose (sterilizing separately) 20g, peptone 20g, yeast extract 10g, seawater 1L, the cooling paraxin that adds 1ml50mg/ml after sterilizing.Cultivate after 48 hours for 30 ℃, picking growth bacterium colony, purifying is also preserved, and obtains isolate.
Above-mentioned isolate is incubated in liquid YPD, gets respectively 1 μ L dibbling in yeast screening culture medium, this screening culture medium is that prawn culturing waste media is dull and stereotyped, its preparation method is: shrimp argol powder 20g, agar powder 20g, seawater 1L, slightly cold after sterilizing, add the paraxin of 1ml50mg/ml.The object of this screening is that acquisition can be at the yeast specie of prawn culturing waste media Fast Growth, and separated yeast will have the ability of utilizing fast livestock waste under low sugar or sugar-free condition.Cultivate after 24 hours for 30 ℃, record growth bacterium colony, chooses that a wherein strain growth is fast, the large bacterial strain of bacterium colony is recorded as Y2.
Colony morphology characteristic and the cell morphological characteristic of Candida tropicalis (Candida tropicalis) Y2: bacterium colony is cultivated 24 hours for white on YPD culture medium flat plate, after 72 hours, be white, the circular projection of bacterium colony, dry, smooth surface or part have wrinkle, tarnish, easily picking, there is wine flavour, cell is spherical to oval, and single or multiple cells are connected, and see sprout (as shown in Figure 1).
(2), Candida tropicalis (Candida tropicalis) Y2 Molecular Identification
The primer that employing is anchored to fungi 18S rDNA gene fragment, carries out pcr amplification, and PCR product is checked order.Primer sequence is: EF3:5 '-TCCTCTAAATGACCAAGTTTG-3 '; EF4:5 '-GGAAGGGRTGTATTTATTAG-3 '.
Bacterial strain Y2 18S rDNA sequence is as shown in SEQ ID NO.1.Through blast comparison and germline generation analysis, show: the 18S rDNA sequence relation of the 18S rDNA sequence of Y2 bacterial strain and a plurality of Candida tropicalis strains is nearest, and similarity reaches 100%, and comparison score is also very high.The stammbaum that 18S rDNA sequence based on Y2 bacterial strain and similar sequences thereof build also shows, the 18S rDNA sequence of Y2 bacterial strain and Candida tropicalis (JQ008834) relation are nearest, 92% the supporting rate of take is gathered as one, confirm that this yeast is the new bacterial strain of Candida tropicalis, called after candida tropicalis (Candida tropicalis) Y2, this bacterial classification has been preserved in Chinese Typical Representative culture collection center (CCTCC) on December 29th, 2013, address: Wuhan, China city Wuhan University, deposit number: CCTCC No:M2013726.
Two, Candida tropicalis (Candida tropicalis) Y2 Analysis of Nutritional
Adopt Kjeldahl determination (GB/T6432-94) to measure the protein content of Candida tropicalis (Candida tropicalis) Y2, when cultivating 2d, crude protein content reaches 56.1%, and the main component that candida tropicalis Y2 is described is protein.The protein content of this bacterial strain is almost 2 times of content (29%) that are generally used for protein in bread yeast (Saccharomyces cerevisiae) that single cell protein produces.
With liquid phase chromatography (GB/T18246-2000), measuring Candida tropicalis (Candida tropicalis) Y2 bacterial strain amino acid forms.Its amino acid forms in Table 1, and result shows: total hydrolysis amino acid accounts for 45.5%% of dry cell weight, wherein must account for 49.5% of total amino acid by amino acid.In aquatic animal feed, Methionin is common limiting amino acid, but the Methionin of Candida tropicalis (Candida tropicalis) Y2 content in must amino acid is the highest.Candida tropicalis (Candida tropicalis) Y2 is similar to the amino acid compositional model of Stichopus variegatus (Sempen) (Stichopus herrmanni), Environment of Litopenaeus vannamei Low (Litopenaeus vannamei).It is 84.1 that Candida tropicalis (Candida tropicalis) Y2 forms calculating biological value to the amino acid of Stichopus variegatus (Sempen), and it is 82.4 that Environment of Litopenaeus vannamei Low amino acid is become to calculate biological value.This result shows that Candida tropicalis (Candida tropicalis) Y2 has good application potential as aquatic feed.
Table 1 candida tropicalis Y2 bacterial strain amino acid form and with aquatic living things comparison
aten kinds of necessary amino acid in aquatic animal;
bnecessary amino acid whose summation;
cthe aminoacids content reference of Stichopus variegatus (Sempen) (Wen etc., 2010);
dthe aminoacids content reference of Environment of Litopenaeus vannamei Low (Tacon etc., 2002).
With vapor-phase chromatography (GB/T17377-2008), measure Candida tropicalis (Candida tropicalis) Y2 lipid acid and form, the results are shown in Table 2.From table, in the lipid acid of Candida tropicalis (Candida tropicalis) Y2, oleic acid (C18:1) content is the highest, accounts for 37.2% of total fatty acids, is secondly that palmitinic acid (C16:0) accounts for 19.7% of total fatty acids.Unsaturated fatty acid content is respectively up to 53.8%, Candida tropicalis (Candida tropicalis) Y2 polyunsaturated fatty acid accounting 12.1%.The lipid acid proportion of composing of Candida tropicalis (Candida tropicalis) Y2 is suitable as can be seen here, has higher nutritive value.
The lipid acid of table 2 candida tropicalis Y2 forms (accounting for the per-cent of total fatty acids)
athe summation of saturated fatty acid;
bthe summation of monounsaturated fatty acids;
cthe summation of polyunsaturated fatty acid;
dthe summation of unsaturated fatty acids; N.D. do not detect.
Three, Candida tropicalis (Candida tropicalis) Y2 solid state fermentation Grouper cultivating refuse biomass statistics
The Grouper cultivating refuse 2g that gets oven dry, adds water 100ml, and adding respectively final concentration is 0 ‰, 2.5 ‰, 5 ‰, 10 ‰ sucrose, after sterilizing is cooling, inoculates respectively Candida tropicalis (Candida tropicalis) Y21ml of incubated overnight.30 ℃ of shaking culture 48h.Get 1ml nutrient solution, do after gradient dilution, candida tropicalis Y2 biomass in the dull and stereotyped calculating of coating YPD livestock waste.The results are shown in Table 3.
Table 3. candida tropicalis Y2 solid state fermentation Grouper cultivating refuse biomass
athis average biomass is the calculation result of three parallel sample.
From table 3, while adding 2.5 ‰ sucrose in livestock waste, biomass reaches 4.05 * 108cfu/g refuse, but when sucrose concentration is too high, causes on the contrary the biomass of Candida tropicalis (Candida tropicalis) Y2 to decline.This sugared concentration of having relatively high expectations from other yeast growth is obviously different.When Candida tropicalis (Candida tropicalis) Y2 separated, we also find that it can not add Fast Growth on the prawn culturing waste media flat board in any sugared source, and other yeast is not grown or poor growth.This result shows, Candida tropicalis (Candida tropicalis) Y2 requires lowly to sugar, is particularly suitable for aquaculture refuse that sugar lacks relatively and the recycling treatment of waste water.
Four, the application of Candida tropicalis (Candida tropicalis) Y2 in sea farming changing waste into resources is disposed
Residual bait, collecting dung by obtaining through physical filtering in industrial circulating water marine fish culture system, dry, more further dry and sterilize through 80 ℃ of high temperature, standby.
Candida tropicalis (Candida tropicalis) Y2 is inoculated in to YPD substratum, and 30 ℃ of incubated overnight, as seed culture fluid.
The livestock waste of above-mentioned dried disinfecting is added to the seawater of sterilization with mass ratio 1:10, do not add sucrose.Again with 2%(V/W) ratio add above-mentioned seed culture fluid, mix.Room temperature fermentation 48h, during repeatedly stir as far as possible.After natural subsidence, abandon supernatant, naturally dry, be sea farming refuse tunning, thereby reach livestock waste higher value application object.After fermentative processing, candida tropicalis (Candida tropicalis) Y2 amount reproduction, utilizes the protein of livestock waste accumulation self, so has improved the value of again utilizing as nutrition of livestock waste; Simultaneously, sea farming refuse tunning presents obvious fermenting aroma, can play the local flavor when improving livestock waste recycling and making bait, improve animal ingestion desire, sea farming refuse tunning can directly be eaten the bait of economic animal as the saprophages such as sea cucumber, shellfish and filter.
Five, the application of candida tropicalis (Candida tropicalis) Y2 in marine culture wastewater disposal of resources
Candida tropicalis (Candida tropicalis) Y2 is inoculated in to YPD substratum, and 30 ℃ of incubated overnight, as seed culture fluid.The waste water that prawn intensive culture system central blow-down system is discharged, focuses on recycling treatment pond, until natural subsidence, after 24 hours, with water pump, absorbs the unnecessary clear water in upper strata, only leaves a small amount of sewage and the residual bait ight soil of bottom.Pass into ozone disinfection, detoxicating is placed 24 hours after processing, during stir Chi Shui repeatedly, to help into water body aeration.With 2%(V/V) ratio add seed culture fluid, do not add sucrose, fully stir and mix, room temperature fermentation 48 hours, during repeatedly stir as far as possible.
After fermentation ends, stir Chi Shui, with water pump, extracting respectively Chi Shui out has respectively the cylinder mould of 10 orders, 20 orders, 40 mesh sieve thin,tough silk by hoop.Because the individual relative bacterium of candida tropicalis (Candida tropicalis) Y2 wants large, water body after filtration was through natural subsidence 4 hours, candida tropicalis (Candida tropicalis) Y2 natural subsidence is to container bottom, absorb upper strata seawater, the white lotion of lower floor's thickness is marine culture wastewater tunning.
This marine culture wastewater tunning can be directly used in the aquatic animal of throwing something and feeding, and after also can being further dried, is packaged into merchandise sales.
Embodiment bis-:
Residual bait, collecting dung by obtaining through physical filtering in industrial circulating water marine fish culture system, dry, more further dry and sterilize through 80 ℃ of high temperature, standby.
Candida tropicalis (Candida tropicalis) Y2 is inoculated in to YPD substratum, and 30 ℃ of incubated overnight, as seed culture fluid.
The livestock waste of above-mentioned dried disinfecting is added to the seawater of sterilization with mass ratio 1:5, according to mass volume ratio, add 5 ‰ sucrose.Again with 5%(V/W) ratio add above-mentioned seed culture fluid, mix.Room temperature fermentation 24h, during repeatedly stir as far as possible.After natural subsidence, abandon supernatant, naturally dry, be sea farming refuse tunning, thereby reach livestock waste higher value application object.After fermentative processing, candida tropicalis (Candida tropicalis) Y2 amount reproduction, utilizes the protein of livestock waste accumulation self, so has improved the value of again utilizing as nutrition of livestock waste; Simultaneously, sea farming refuse tunning presents obvious fermenting aroma, can play the local flavor when improving livestock waste recycling and making bait, improve animal ingestion desire, sea farming refuse tunning can directly be eaten the bait of economic animal as the saprophages such as sea cucumber, shellfish and filter.
Embodiment tri-:
Candida tropicalis (Candida tropicalis) Y2 is inoculated in to YPD substratum, and 30 ℃ of incubated overnight, as seed culture fluid.The waste water that prawn intensive culture system central blow-down system is discharged, focuses on recycling treatment pond, until natural subsidence, after 24 hours, with water pump, absorbs the unnecessary clear water in upper strata, only leaves a small amount of sewage and the residual bait ight soil of bottom.Pass into ozone disinfection, detoxicating is placed 24 hours after processing, during stir Chi Shui repeatedly, to help into water body aeration.With 5%(V/V) ratio add seed culture fluid, according to mass volume ratio, add 5 ‰ sucrose, fully stir and mix, room temperature fermentation 24 hours, during repeatedly stir as far as possible.
After fermentation ends, stir Chi Shui, with water pump, extracting respectively Chi Shui out has respectively the cylinder mould of 10 orders, 20 orders, 40 mesh sieve thin,tough silk by hoop.Because the individual relative bacterium of candida tropicalis (Candida tropicalis) Y2 wants large, water body after filtration was through natural subsidence 4 hours, candida tropicalis (Candida tropicalis) Y2 natural subsidence is to container bottom, absorb upper strata seawater, the white lotion of lower floor's thickness is marine culture wastewater tunning.
This marine culture wastewater tunning can be directly used in the aquatic animal of throwing something and feeding, and after also can being further dried, is packaged into merchandise sales.
Claims (6)
1. Candida tropicalis (Candida tropicalis) Y2, its deposit number: CCTCC No:M2013726.
2. the application of Candida tropicalis claimed in claim 1 (Candida tropicalis) Y2 in the functional additive of bait of producing aquatic animal.
3. the application of Candida tropicalis claimed in claim 1 (Candida tropicalis) Y2 in sea farming changing waste into resources is disposed.
4. the application of Candida tropicalis according to claim 2 (Candida tropicalis) Y2 in sea farming changing waste into resources is disposed, and be characterised in that, concrete grammar is: first Candida tropicalis Y2 is incubated in YPD substratum, form seed liquor, every 1 kilogram of livestock waste adds 5～10 kilograms of sterilization seawater, by seed liquor with 2～5%(V/W) ratio add wherein, and supplement 0～5 ‰ sucrose as carbon source according to mass volume ratio, room temperature was cultivated through 24～48 hours, natural subsidence again, abandon supernatant, the air-dry sea farming refuse tunning that is.
5. the application of Candida tropicalis claimed in claim 1 (Candida tropicalis) Y2 in marine culture wastewater disposal of resources.
6. the application of Candida tropicalis according to claim 4 (Candida tropicalis) Y2 in marine culture wastewater disposal of resources, and be characterised in that, concrete grammar is: first Candida tropicalis (Candida tropicalis) Y2 is incubated in YPD substratum, form seed liquor, by seed liquor with 2～5%(V/V) ratio add in sterile-processed marine culture wastewater, according to mass volume ratio, add 0～5 ‰ sucrose as carbon source, fully stir and mix, room temperature was cultivated through 24～48 hours, after fermentation ends, stir Chi Shui, with water pump, extract respectively Chi Shui out and by hoop, have 10 orders respectively, 20 orders, the cylinder mould of 40 mesh sieve thin,tough silk, water body after filtration was through natural subsidence 4 hours, the white lotion that obtains thickness is marine culture wastewater tunning.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410072606.6A CN104031851B (en) | 2014-02-28 | 2014-02-28 | A kind of Candida tropicalis Y2 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410072606.6A CN104031851B (en) | 2014-02-28 | 2014-02-28 | A kind of Candida tropicalis Y2 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104031851A true CN104031851A (en) | 2014-09-10 |
| CN104031851B CN104031851B (en) | 2016-08-17 |
Family
ID=51462849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410072606.6A Expired - Fee Related CN104031851B (en) | 2014-02-28 | 2014-02-28 | A kind of Candida tropicalis Y2 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104031851B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104845905A (en) * | 2015-03-26 | 2015-08-19 | 北京北华中清环境工程技术有限公司 | High-efficiency composite inocula for removing salad oil-containing waste water COD and application thereof |
| CN113088459A (en) * | 2021-04-25 | 2021-07-09 | 天津科技大学 | Heat-resistant high-yield candida tropicalis as well as preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926409A (en) * | 2008-11-28 | 2010-12-29 | 薛晓宁 | Feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and processing method thereof |
| CN102220262A (en) * | 2011-04-29 | 2011-10-19 | 哈尔滨恩瑞科技发展有限公司 | Composite microbiological preparation and preparation method and use thereof |
-
2014
- 2014-02-28 CN CN201410072606.6A patent/CN104031851B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101926409A (en) * | 2008-11-28 | 2010-12-29 | 薛晓宁 | Feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and processing method thereof |
| CN102220262A (en) * | 2011-04-29 | 2011-10-19 | 哈尔滨恩瑞科技发展有限公司 | Composite microbiological preparation and preparation method and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| R.RHISHIPAL等: "Selection of Marine Yeasts for the Generation of Single Cell Protein from Prawn-shell Waste", 《BIORESOURCE TECHNOLOGY》 * |
| 常仁亮 等: "硒酵母的培养及其养虾效果", 《水产学报》 * |
| 高玲美: "高蛋白海洋酵母的初步研究", 《中国博士学位论文全文数据库基础科学辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104845905A (en) * | 2015-03-26 | 2015-08-19 | 北京北华中清环境工程技术有限公司 | High-efficiency composite inocula for removing salad oil-containing waste water COD and application thereof |
| CN104845905B (en) * | 2015-03-26 | 2018-02-16 | 北京北华中清环境工程技术有限公司 | The high efficiency composition microbial inoculum of removal COD containing salad oil wastewater a kind of and its application |
| CN113088459A (en) * | 2021-04-25 | 2021-07-09 | 天津科技大学 | Heat-resistant high-yield candida tropicalis as well as preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104031851B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103497906B (en) | Microecological preparation, preparation method, and applications thereof | |
| CN106867933A (en) | The probiotics of purification of water quality and preparation and application in being cultivated to Environment of Litopenaeus vannamei Low | |
| CN103602608A (en) | Application of bacillus pumilus in prawn culture | |
| Wada et al. | Single-cell protein production from purple non-sulphur bacteria-based wastewater treatment | |
| CN107974421A (en) | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum | |
| CN116439174B (en) | Seedling cultivation method for Babylonia | |
| CN103911297B (en) | A kind of Rhodosporidium toruloides Y0 and application thereof | |
| EP1142985A1 (en) | Chlorophyll-rich and salt-resistant chlorella | |
| CN114468121B (en) | Method for fermenting food industry leftovers by using fermentation agent containing Bacillus belgii | |
| KR101302705B1 (en) | Method for preparing pork cutlet using capsosiphon fulvescens | |
| CN103211088A (en) | Preparation method of sea cucumber bait | |
| CN103740615A (en) | Photosynthetic bacteria SC01 as well as fast cultivation method and application thereof | |
| CN104031851B (en) | A kind of Candida tropicalis Y2 and application thereof | |
| CN110734867B (en) | Deep-sea-derived yeast Q7-7 and application thereof | |
| CN110800888A (en) | Composition for culturing plankton, preparation method and application thereof | |
| CN114376113B (en) | Bait special for water spider and produced by raw materials prepared from Bacillus belgii and application thereof | |
| CN106957804A (en) | Prevent and treat the microbe controlling method that drip emitter is blocked | |
| CN110012852A (en) | A kind of method for improving of offshore floating cage cultured large yellow croaker adult fish quality | |
| CN114381394B (en) | Bacillus beiLeisi strain and feed raw material leavening agent for water spider | |
| KR101323957B1 (en) | Novel Chlorella vulgaris capable low temperature growth and use thereof | |
| CN110923156B (en) | Yeast Y5 for resisting aquatic pathogenic bacteria and producing lipase, separation method and application thereof, feed additive and preparation method thereof | |
| KR101323873B1 (en) | Novel Nannochloropsis sp. capable high growth and use thereof | |
| CN110089623B (en) | Scenedesmus and its application as bait/bait additive | |
| KR101323887B1 (en) | Novel Nannochloris sp. Capable High Temperature Growth and Use Thereof | |
| CN112641012A (en) | Method for preparing large yellow croaker biological fermentation feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20210228 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |