CN104031851B - A kind of Candida tropicalis Y2 and application thereof - Google Patents
A kind of Candida tropicalis Y2 and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of Candida tropicalis Y2 and application thereof.Candida tropicalis (Candida tropicalis) Y2 is preserved in China typical culture collection center (CCTCC) on the 29th in December in 2013, address: Wuhan University of Wuhan, China city, deposit number: CCTCC No:M2013726.Candida tropicalis (Candida tropicalis) Y2 of the present invention has abundant nutrition, can be applicable to produce the functional additive of bait of aquatic animal, can originate with sea-farming refuse or waste water for main nutrient simultaneously, in the case of according to mass volume ratio interpolation 0～5 ‰ sucrose as supplementary carbon source, quickly grow in sea-farming refuse or waste water.Its livestock waste tunning can be as saprophage or the feedstuff of filter-feeding aquatic animal；Its breeding wastewater tunning can be as the feed additive of aquatic animal.
Description
Technical field:
The invention belongs to technical field of microbe application, be specifically related to a kind of Candida tropicalis Y2 and application thereof.
Background technology:
Yeast nutrition enriches, is of moderate size, and is a lot of biological excellent natural baits, and yeast is also a class saprophytic bacteria simultaneously, can
To decompose many kinds of substance, utilize the livestock wastes such as the Organic substance in breeding water body, ammonia nitrogen, hydrogen sulfide, and form dominant population,
Effectively suppress other harmful microbe to grow, play the effect of Biological control.Progress recently as aquaculture technology
And aquatic animal nutritional need understanding is goed deep into, yeast application in aquaculture is more and more extensive.
But the yeast that can be applicable to sea-farming in the market is the most considerably less, conventional yeast remains and comes from food brewing
Bakery yeast (Saccharomyces cerevisiae), this yeast coming from terrestial enviornment can not adapt to briny environment,
Therefore can not play the effect of purifying sea water water quality, it also lacks the report of science for the impact of marine fishery growth of animal.Base
In this kind of situation, it is adapted to the unicellular yeast of marine environment for marine aquaculture in the urgent need to separating.On the other hand, ferment
In female large-scale culture, it usually needs some agricultural byproducts, as raw material, add production cost, it is therefore desirable to explore new
Substitute nutrient source, with cost-effective.
Additionally, the scale of China's sea-farming in the last thirty years and yield are continuously increased, the most occupy first place in the world, but quickly send out
Also serious problem of environmental pollution is brought while exhibition.Along with the raising of people's environmental consciousness, government is for the dirt of aquaculture
The strictest control, therefore development environment friendly aquaculture pattern are implemented in thing discharge, walk the development of continuable aquaculture
Road becomes inexorable trend.At present, although livestock waste is separated from water body by some cultivating wastewater purification methods, but
Can not effectively utilize livestock waste.How to carry out breeding wastewater, the recycling of refuse is that development environment friendly Aquatic product is supported
Grow the problem that pattern have to solve.
Summary of the invention:
First purpose of the present invention is to provide one to be had high nutritive value, efficiently utilizes sea-farming refuse, waste water and can change
Candida tropicalis (Candida tropicalis) Y2 of kind fermentation waste local flavor, this bacterium is preserved in December in 2013 on the 29th
China typical culture collection center (CCTCC), address: Wuhan University of Wuhan, China city, deposit number: CCTCC No:
M2013726。
Candida tropicalis of the present invention (Candida tropicalis) Y2 is located away from ShenZhen,GuangDong gold Stromateoides argenteus plant row
Dirty mouth.Yeast isolation culture medium is YPD yeast special culture media.Candida tropicalis (Candida tropicalis) Y2
Screening culture medium is prawn culturing waste media.The purpose of this screening is that acquisition can be at prawn culturing waste media fast-growth
Yeast specie, the yeast of separation utilizes the ability of livestock waste under the conditions of low sugar or sugar-free by having.
The colony morphology characteristic of Candida tropicalis (Candida tropicalis) Y2 and cell morphological characteristic: bacterium colony is at YPD
Cultivating on culture medium flat plate 24 hours is white, is white after 72 hours, bacterium colony circle projection, dry, smooth surface or portion
Divide and have wrinkle, matt, easy picking, there is wine flavour, cell is spherical to ellipse, and single or multiple cells are connected, see and sprout
(as shown in Figure 1).
Candida tropicalis (Candida tropicalis) Y2 growth conditions in prawn culturing waste media is cultivated with YPD
Base class is seemingly.
Extract test kit (purchased from Takara, China) with Yeast genome and extract DNA.PCR expands and checks order 18S rDNA
Fragment, its 18S rDNA sequence is as shown in SEQ ID NO.1, by 18S rDNA sequence by the BLAST in GeneBank
Software is compared with the sequence in GeneBank data base, Candida tropicalis (Candida tropicalis) Y2 bacterial strain
18S rDNA sequence is nearest with the 18S rDNA sequence relation of multiple Candida tropicalis strains, and similarity reaches 100%, and ratio
The highest to score.18S rDNA sequence based on Candida tropicalis (Candida tropicalis) Y2 bacterial strain and similar
The stammbaum of sequence construct displays that, the 18S rDNA sequence of Candida tropicalis (Candida tropicalis) Y2 bacterial strain
Row are nearest with Candida tropicalis (JQ008834) relation, and gathering with the supporting rate of 92% is one, confirms that this yeast is
The new strains of Candida tropicalis, named candida tropicalis (Candida tropicalis) Y2, this strain is in 2013
On December is preserved in China typical culture collection center (CCTCC) for 29, address: Wuhan University of Wuhan, China city, protects
Hide and number: CCTCC No:M2013726.
Through Candida tropicalis (Candida tropicalis) Y2 of the measuring present invention there is abundant nutrition, therefore,
Second object of the present invention is to provide Candida tropicalis (Candida tropicalis) Y2 and is producing aquatic animal
Application in functional additive of bait.
Fermented livestock waste is tested, and in the case of adding 2.5 ‰ sucrose, cultivates the false silk ferment in the torrid zone of the present invention in livestock waste
After female bacterium (Candida tropicalis) Y2 48h, Biomass reaches 4.05 × 108cfu/g refuse.When but sucrose concentration is too high
The Biomass causing candida tropicalis (Candida tropicalis) Y2 on the contrary declines.This requires higher with other yeast growth
Sugared concentration significantly different.When the separation of candida tropicalis (Candida tropicalis) Y2, find that it can not add
Add fast-growth on the prawn culturing waste media flat board of any sugar source, and other yeast does not grows or poor growth.This knot
Fruit shows, candida tropicalis (Candida tropicalis) Y2 is low to the requirement of sugar, is particularly suitable for sugar and relatively lacks
Aquaculture refuse and the disposal of resources of waste water.
Therefore, third object of the present invention be to provide candida tropicalis (Candida tropicalis) Y2 sea-farming give up
Application in thing disposal of resources.
Described candida tropicalis (Candida tropicalis) Y2 application in sea-farming changing waste into resources is disposed, tool
Body method is: be first incubated in YPD culture medium by Candida tropicalis Y2, forms seed liquor.Every 1 kilogram of livestock waste
Add 5～10 kilograms of sterilization sea water, seed liquor is added wherein with the ratio of 2～5% (V/W), and mends according to mass volume ratio
Fill the sucrose of 0～5 ‰ as carbon source.Room temperature was cultivated through 24～48 hours, then natural subsidence, abandoned supernatant, and the air-dried sea water that is is supported
Grow refuse tunning, thus reach livestock waste higher value application purpose.After fermented process, candida tropicalis (Candida
Tropicalis) Y2 amount reproduction, utilizes the protein of livestock waste accumulation self, therefore improves the conduct again of livestock waste
The value that nutrient utilizes；Meanwhile, sea-farming refuse tunning presents obvious fermenting aroma, can play improvement and support
Grow local flavor when refuse reclamation makees bait, improve animal ingestion desire, sea-farming refuse tunning can directly as Stichopus japonicus,
The saprophages such as shellfish and the bait of filter food economic animal.
Fourth object of the present invention is to provide candida tropicalis (Candida tropicalis) Y2 in marine culture wastewater resource
Change the application in disposing.
Described candida tropicalis (Candida tropicalis) Y2 application in marine culture wastewater disposal of resources, tool
Body method is: be first incubated in YPD culture medium by Candida tropicalis (Candida tropicalis) Y2, forms seed liquor.
Seed liquor is added in sterile-processed marine culture wastewater with the ratio of 2～5% (V/V).Add according to mass volume ratio
The sucrose of 0～5 ‰, as carbon source, fully stirs mixing, and room temperature was cultivated through 24～48 hours, after fermentation ends, stirs water, with
Water extracted respectively out by water pump has 10 mesh, 20 mesh, the cylinder mould of 40 mesh sieve thin,tough silk by hoop respectively, and the water body after filtration is through natural subsidence
4 hours, the white mastic obtaining thickness was marine culture wastewater tunning, and the marine culture wastewater tunning obtained can
To be directly used in aquatic animal of throwing something and feeding, it is also possible to be packaged into merchandise sales after being further dried, thus it is high to reach breeding wastewater
Value utilizes purpose.
Candida tropicalis (Candida tropicalis) Y2 of the present invention is preserved in Chinese Typical Representative training on the 29th in December in 2013
Support thing preservation center (CCTCC), address: Wuhan University of Wuhan, China city, deposit number: CCTCC No:M2013726.
Accompanying drawing illustrates:
Fig. 1 is that Candida tropicalis (Candida tropicalis) Y2 cultivates and cellular morphology figure.Wherein a: Candida tropicalis
(Candida tropicalis) Y2 colonial morphology on YPD flat board；B: Candida tropicalis (Candida tropicalis)
Y2 cellular morphology, cell is spherical or elliposoidal, gemmation, scale=10 μm；C: Candida tropicalis (Candida
Tropicalis) Y2 bacterium solution is white；D: Candida tropicalis (Candida tropicalis) Y2 bacterial strain is at prawn culturing refuse
Colonial morphology on flat board.
Detailed description of the invention:
Following example are to further illustrate the present invention rather than limitation of the present invention.Method used and technology such as without
Special instruction is method and the technology of routine.
Embodiment 1:
One, Candida tropicalis (Candida tropicalis) Y2 separation, screen and identify
(1), Candida tropicalis (Candida tropicalis) separation of Y2, screening
Take ShenZhen,GuangDong gold Stromateoides argenteus plant discharge outlet mud 1g, add sterilizing sea water 10ml, take 200 μ L after mixing and be coated with ferment
Female bacterium isolation medium (YPD) flat board, YPD is yeast special culture media, and preparation method is: glucose (independent sterilizing)
20g, peptone 20g, yeast extract 10g, sea water 1L, after sterilizing, cooling adds the chloromycetin of 1ml50mg/ml.30
DEG C cultivating after 48 hours, picking growth bacterium colony, purification also preserves, and obtains separating bacterium.
Above-mentioned separation bacterium is incubated in liquid YPD, takes 1 μ L dibbling respectively in yeast screening culture medium, this screening culture medium
For prawn culturing waste media flat board, its preparation method is: shrimp argol powder 20g, agar powder 20g, sea water 1L, after sterilizing
The coldest, add the chloromycetin of 1ml50mg/ml.The purpose of this screening is that acquisition can be at prawn culturing waste media fast-growth
Yeast specie, the yeast of separation quickly utilizes the ability of livestock waste under the conditions of low sugar or sugar-free by having.Cultivate 24 for 30 DEG C
After hour, record growth bacterium colony, choose that a wherein strain growth is fast, the big bacterial strain of bacterium colony is recorded as Y2.
The colony morphology characteristic of Candida tropicalis (Candida tropicalis) Y2 and cell morphological characteristic: bacterium colony is at YPD
Cultivating on culture medium flat plate 24 hours is white, is white after 72 hours, bacterium colony circle projection, dry, smooth surface or portion
Divide and have wrinkle, matt, easy picking, there is wine flavour, cell is spherical to ellipse, and single or multiple cells are connected, see and sprout
(as shown in Figure 1).
(2), Candida tropicalis (Candida tropicalis) Y2 Molecular Identification
Use the primer being anchored to fungus 18S rDNA genetic fragment, carry out PCR amplification, and PCR primer is checked order.Primer
Sequence is: EF3:5 '-TCCTCTAAATGACCAAGTTTG-3 '；EF4:5’
-GGAAGGGRTGTATTTATTAG-3’。
Bacterial strain Y2 18S rDNA sequence is as shown in SEQ ID NO.1.Show through blast comparison and Phylogenetic Analysis: Y2 bacterium
The 18S rDNA sequence of strain is nearest with the 18S rDNA sequence relation of multiple Candida tropicalis strains, and similarity reaches 100%,
And comparison score is the highest.The stammbaum that 18S rDNA sequence based on Y2 bacterial strain and similar sequences thereof build displays that,
The 18S rDNA sequence of Y2 bacterial strain and Candida tropicalis (JQ008834) relation are nearest, gather with the supporting rate of 92% and are
One, confirm the new strains that this yeast is Candida tropicalis, named candida tropicalis (Candida tropicalis)
Y2, this strain is preserved in China typical culture collection center (CCTCC) on the 29th in December in 2013, address: China
Wuhan University of Wuhan City, deposit number: CCTCC No:M2013726.
Two, Candida tropicalis (Candida tropicalis) Y2 Analysis of Nutritive Composition
Use Kjeldahl's method (GB/T6432-94) that the protein of Candida tropicalis (Candida tropicalis) Y2 is contained
Amount is measured, and when cultivating 2d, crude protein content reaches 56.1%, illustrates that candida tropicalis Y2's is mainly composed of egg
White matter.The protein content of this bacterial strain is almost generally used for the bakery yeast (Saccharomyces that single cell protein produces
Cerevisiae) in 2 times of the content (29%) of protein.
Candida tropicalis (Candida tropicalis) Y2 bacterial strain ammonia is measured with liquid chromatography (GB/T18246-2000)
Base acid forms.Its aminoacid composition is shown in Table 1, and result shows: total hydrolysis amino acid accounts for the 45.5%% of dry cell weight, the most necessary
Aminoacid accounts for the 49.5% of total amino acids.In aquatic animal feed, lysine is common limiting amino acid, but the false silk in the torrid zone
The lysine of yeast (Candida tropicalis) Y2 must content be the highest in aminoacid.Candida tropicalis
(Candida tropicalis) Y2 and Stichopus variegatus (Sempen) (Stichopus herrmanni), Litopenaeus vannamei (Litopenaeus vannamei)
Aminoacid compositional model similar.Candida tropicalis (Candida tropicalis) Y2 forms meter to the aminoacid of Stichopus variegatus (Sempen)
Calculating biological value is 84.1, and becoming to calculate biological value to Litopenaeus vannamei aminoacid is 82.4.This result shows Candida tropicalis
(Candida tropicalis) Y2 has good application potential as aquatic feed.
Table 1 candida tropicalis Y2 bacterial strain aminoacid forms and compares with aquatic biological
aIn aquatic animal ten kinds must aminoacid；bMust amino acid whose summation；cThe amino acid content list of references of Stichopus variegatus (Sempen) (Wen etc.,
2010)；dThe amino acid content list of references (Tacon etc., 2002) of Litopenaeus vannamei.
Candida tropicalis (Candida tropicalis) Y2 fatty acid is measured with gas chromatography (GB/T17377-2008)
Composition, the results are shown in Table 2.From table, oleic acid (C18:1) in the fatty acid of Candida tropicalis (Candida tropicalis) Y2
Content is the highest, accounts for the 37.2% of total fatty acids, next to that Palmic acid (C16:0) accounts for the 19.7% of total fatty acids.Unsaturated fatty acids
Acid content is up to 53.8% respectively, Candida tropicalis (Candida tropicalis) Y2 polyunsaturated fatty acid accounting 12.1%.
The fatty acid proportion of composing of Candida tropicalis (Candida tropicalis) Y2 is suitable as can be seen here, has higher nutrition
It is worth.
Fatty acid composition (accounting for the percentage ratio of total fatty acids) of table 2 candida tropicalis Y2
aThe summation of satisfied fatty acid；bThe summation of monounsaturated fatty acid；cThe summation of polyunsaturated fatty acid；dThe summation of unsaturated fatty acid；
N.D. do not detect.
Three, Candida tropicalis (Candida tropicalis) Y2 solid fermentation Grouper cultivating refuse biomass statistics
Taking the Grouper cultivating refuse 2g of drying, add water 100ml, is separately added into final concentration of 0 ‰, 2.5 ‰, 5 ‰, 10 ‰
Sucrose, after sterilizing cooling, inoculates Candida tropicalis (Candida tropicalis) the Y2 1ml of incubated overnight respectively.30℃
Shaken cultivation 48h.Taking 1ml culture fluid, after doing gradient dilution, coating YPD flat board calculates candida tropicalis in livestock waste
Y2 Biomass.The results are shown in Table 3.
Table 3. candida tropicalis Y2 solid fermentation Grouper cultivating refuse Biomass
From table 3, when adding 2.5 ‰ sucrose in livestock waste, Biomass reaches 4.05 × 108cfu/g refuse, but sugarcane
The Biomass of Candida tropicalis (Candida tropicalis) Y2 is caused to decline on the contrary during sugar excessive concentration.This is with other ferment
Female growth requires that higher sugared concentration is significantly different.When the separation of Candida tropicalis (Candida tropicalis) Y2,
It has been found that it can be without fast-growth on the prawn culturing waste media flat board of any sugar source, and other yeast is not
Growth or poor growth.This result shows, Candida tropicalis (Candida tropicalis) Y2 is low to the requirement of sugar,
It is particularly suitable for aquaculture refuse and the recycling treatment of waste water that sugar lacks relatively.
Four, Candida tropicalis (Candida tropicalis) Y2 application in sea-farming changing waste into resources is disposed
By the residual bait obtained through physical filtering in industrial circulating water marine fish culture system, collecting dung, dry, then warp
High temperature 80 DEG C is further dried and is sterilized, standby.
Candida tropicalis (Candida tropicalis) Y2 is inoculated in YPD culture medium, and 30 DEG C of incubated overnight, as kind
Sub-culture fluid.
The livestock waste of above-mentioned dried disinfecting is added the sea water of sterilization, without sucrose with mass ratio 1:10.Again with 2% (V/W)
Ratio add above-mentioned seed culture fluid, mix homogeneously.Room temperature fermentation 48h, period many times stirs.After natural subsidence,
Abandon supernatant, naturally dry, be i.e. sea-farming refuse tunning, thus reach livestock waste higher value application purpose.Warp
After fermentation process, candida tropicalis (Candida tropicalis) Y2 amount reproduction, utilize the egg of livestock waste accumulation self
White matter, therefore improves the value again utilized of livestock waste as nutrient；Meanwhile, sea-farming refuse tunning in
Reveal obvious fermenting aroma, the local flavor improved when livestock waste recycling makees bait can be played, improve animal ingestion desire,
Sea-farming refuse tunning can be directly as the saprophage such as Stichopus japonicus, shellfish and the bait of filter food economic animal.
Five, candida tropicalis (Candida tropicalis) Y2 application in marine culture wastewater disposal of resources
Candida tropicalis (Candida tropicalis) Y2 is inoculated in YPD culture medium, and 30 DEG C of incubated overnight, as seed
Culture fluid.The waste water that prawn intensive culture CS central drainage is discharged, focuses on recycling treatment pond, treats natural subsidence
After 24 hours, remove, with Pump Suction, the clear water that upper strata is unnecessary, leave behind a small amount of sewage of bottom and residual bait feces.It is passed through ozone to carry out
Disinfecting, after detoxicating processes, place 24 hours, period stirs water repeatedly, to help into water body aeration.With 2% (V/V)
Ratio add seed culture fluid, be added without sucrose, fully stir mixing, room temperature is fermented 48 hours, and period many times stirs.
After fermentation ends, stirring water, extracting water respectively out with water pump has 10 mesh, 20 mesh, 40 mesh sieve thin,tough silk by hoop respectively
Cylinder mould.Owing to the individual relative antibacterial of candida tropicalis (Candida tropicalis) Y2 wants big, the water body after filtration is through certainly
So sedimentation 4 hours, candida tropicalis (Candida tropicalis) Y2 natural subsidence to container bottom, absorb upper ocean water,
The white mastic of lower floor's thickness is marine culture wastewater tunning.
This marine culture wastewater tunning is used directly for aquatic animal of throwing something and feeding, it is also possible to be packaged into after being further dried
Merchandise sales.
Embodiment two:
By the residual bait obtained through physical filtering in industrial circulating water marine fish culture system, collecting dung, dry, then warp
High temperature 80 DEG C is further dried and is sterilized, standby.
Candida tropicalis (Candida tropicalis) Y2 is inoculated in YPD culture medium, and 30 DEG C of incubated overnight, as kind
Sub-culture fluid.
The livestock waste of above-mentioned dried disinfecting is added with mass ratio 1:5 the sea water of sterilization, adds 5 ‰ sugarcanes according to mass volume ratio
Sugar.Above-mentioned seed culture fluid, mix homogeneously is added again with the ratio of 5% (V/W).Room temperature fermentation 24h, period many times
Agitation.After natural subsidence, abandon supernatant, naturally dry, be sea-farming refuse tunning, thus reach livestock waste
Higher value application purpose.After fermented process, candida tropicalis (Candida tropicalis) Y2 amount reproduction, utilize and support
Grow the protein of Waste buildup self, therefore improve the value again utilized as nutrient of livestock waste；Meanwhile, sea water
Livestock waste tunning presents obvious fermenting aroma, can play the local flavor improved when livestock waste recycling makees bait,
Improving animal ingestion desire, sea-farming refuse tunning can eat economic animal directly as the saprophage such as Stichopus japonicus, shellfish and filter
Bait.
Embodiment three:
Candida tropicalis (Candida tropicalis) Y2 is inoculated in YPD culture medium, and 30 DEG C of incubated overnight, as seed
Culture fluid.The waste water that prawn intensive culture CS central drainage is discharged, focuses on recycling treatment pond, treats natural subsidence
After 24 hours, remove, with Pump Suction, the clear water that upper strata is unnecessary, leave behind a small amount of sewage of bottom and residual bait feces.It is passed through ozone to carry out
Disinfecting, after detoxicating processes, place 24 hours, period stirs water repeatedly, to help into water body aeration.With 5% (V/V)
Ratio add seed culture fluid, add 5 ‰ sucrose according to mass volume ratio, fully stir mixing, room temperature is fermented 24 hours,
Period many times stirs.
After fermentation ends, stirring water, extracting water respectively out with water pump has 10 mesh, 20 mesh, 40 mesh sieve thin,tough silk by hoop respectively
Cylinder mould.Owing to the individual relative antibacterial of candida tropicalis (Candida tropicalis) Y2 wants big, the water body after filtration is through certainly
So sedimentation 4 hours, candida tropicalis (Candida tropicalis) Y2 natural subsidence to container bottom, absorb upper ocean water,
The white mastic of lower floor's thickness i.e. marine culture wastewater tunning.
This marine culture wastewater tunning is used directly for aquatic animal of throwing something and feeding, it is also possible to be packaged into after being further dried
Merchandise sales.
Claims (6)
1. Candida tropicalis (Candida tropicalis) Y2, its deposit number: CCTCC No:M2013726.
2. Candida tropicalis (Candida tropicalis) Y2 described in claim 1 is producing the function of aquatic animal
Application in property additive of bait, described aquatic animal is Stichopus variegatus (Sempen) or Litopenaeus vannamei.
3. Candida tropicalis (Candida tropicalis) Y2 described in claim 1 disposes at sea-farming changing waste into resources
In application.
Candida tropicalis the most according to claim 3 (Candida tropicalis) Y2 is at sea-farming changing waste into resources
Application in disposal, and be characterised by, method particularly includes: first Candida tropicalis Y2 is incubated at YPD culture medium
In, forming seed liquor, every 1 kilogram of livestock waste adds 5～10 kilograms of sterilization sea water, by seed liquor with 2～5%V/W
Ratio is added wherein, and supplements the sucrose of 0～5 ‰ according to mass volume ratio and cultivated through 24～48 hours as carbon source, room temperature,
Natural subsidence again, abandons supernatant, air-dries and is sea-farming refuse tunning.
5. Candida tropicalis (Candida tropicalis) Y2 described in claim 1 is in marine culture wastewater disposal of resources
In application.
Candida tropicalis the most according to claim 5 (Candida tropicalis) Y2 is in marine culture wastewater resource
Application in disposal, and be characterised by, method particularly includes: first by Candida tropicalis (Candida tropicalis) Y2
It is incubated in YPD culture medium, forms seed liquor, seed liquor is added to sterile-processed with the ratio of 2～5%V/V
In marine culture wastewater, according to mass volume ratio add 0～5 ‰ sucrose as carbon source, fully stir mixing, room temperature warp
24～48 hours cultivate, after fermentation ends, stir water, extract out respectively with water pump water respectively by hoop have 10 mesh, 20
Mesh, the cylinder mould of 40 mesh sieve thin,tough silk, the water body after filtration was through natural subsidence 4 hours, and the white mastic obtaining thickness is sea water
Breeding wastewater tunning.
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| CN101926409A (en) * | 2008-11-28 | 2010-12-29 | 薛晓宁 | Feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and processing method thereof |
| CN102220262A (en) * | 2011-04-29 | 2011-10-19 | 哈尔滨恩瑞科技发展有限公司 | Composite microbiological preparation and preparation method and use thereof |
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