CN104003919A - Method for extracting carotenoid from Melaleuca bracteata - Google Patents
Method for extracting carotenoid from Melaleuca bracteata Download PDFInfo
- Publication number
- CN104003919A CN104003919A CN201410239312.8A CN201410239312A CN104003919A CN 104003919 A CN104003919 A CN 104003919A CN 201410239312 A CN201410239312 A CN 201410239312A CN 104003919 A CN104003919 A CN 104003919A
- Authority
- CN
- China
- Prior art keywords
- incense
- carotenoid
- golden color
- ultrasonic
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a method for extracting carotenoid from Melaleuca bracteata. The method comprises the following specific steps: (1) firstly, collecting fresh Melaleuca bracteata leaves, and then, carrying out freeze drying for 30-60 hours in a freeze-drying machine; (2) crushing the freeze-dried Melaleuca bracteata leaves, and sieving with a sieve having the pore size of 40 meshes; (3) then, adding an acetone and petroleum ether mixed extracting solution into the crushed leaves in a material/solution ratio of 1: (15-40); (4) carrying out ultrasonic-assisted extraction, wherein the ultrasonic power is 40-90W, the ultrasonic temperature is 20-70 DEG C, the ultrasonic time is 5-50 minutes, and the time of extraction is 1-4; (5) after the extraction is completed, carrying out centrifugation, and absorbing a supernatant, thereby obtaining a solution containing carotenoid. The method for extracting the carotenoid from the Melaleuca bracteata has the advantages of convenience, quickness and low equipment requirements, and is simple to operate; through extracting the carotenoid by using the method disclosed by the invention, the extraction ratio is high.
Description
Technical field
The present invention relates to technical field of plant extraction, relate in particular to a kind of method of extracting carotenoid from incense with golden color willow.
Background technology
Incense with golden color willow (Melaleuca bracteata cv.Revolution Gold), is again thousand layers of gold, and Myrtaceae white spirit layer belongs to, branch is elongated, one of fine tree species that blade golden yellow or yellow is township afforestation, beautify the environment, are applicable to planting in loke shore limit, river.Pigment in plant leaf comprises chlorophyll, anthocyanidin and carotenoid, and these several pigments shared component and determining positions in cell the color of leaf, and in the time that carotenoid proportion is larger, blade is orange or yellow.Carotenoid is to be synthesized and presented yellow, an orange red and red large class terpene coloring matter by Isoprenoid pathway in organism.Carotenoid is not only as photosynthetic accessory pigment, participate in carrying out luminous energy transmission and Substance Transformation, and be the important natural pigment of a class, give animals and plants with the color and luster such as yellow, orange and red, there is important physiological function, can preventing chronic disease, suppress tumour, anti-oxidant and strengthen human immunity activity etc.
At present, the research of incense with golden color willow is mainly concentrated on the cultivation management aspects such as breediness, the prevention and control of plant diseases, pest control and grafting both at home and abroad, and the extraction of its carotenoid is studied, there is not yet the report that utilizes ultrasonic assisted Extraction to follow the example of extraction.
Summary of the invention
The object of the invention is to provide a kind of method of extracting carotenoid from incense with golden color willow.
The technical scheme that the present invention takes is:
The concrete steps of the method for extracting carotenoid from incense with golden color willow of the present invention are as follows:
(1) first gather fresh incense with golden color willow leaf sheet, then put into Freeze Drying Equipment freeze-drying 30-60h;
(2) the incense with golden color willow leaf sheet after freeze-drying is pulverized, crossed 40 mesh sieves;
(3) then toward pulverize after blade in add acetone and sherwood oil mixed extract, solid-liquid ratio is 1: 15-40;
(4) carry out ultrasonic assisted extraction, ultrasonic power is 40-90W again, and ultrasonic temperature is 20-70 DEG C, and ultrasonic time 5-50min extracts 1-4 time;
(5) extract after, carry out centrifugal, at 4 DEG C, under rotating speed 8000rpm, extract 10min, draw supernatant liquor, can obtain the solution that contains carotenoid.
In step (1), preferably put into Freeze Drying Equipment freeze-drying 48h.
In step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 1-9: 3, be preferably 6: 3.
In step (3), solid-liquid ratio is preferably 1: 36.
In step (3), preferably ultrasonic power is 50W, and ultrasonic temperature is 63 DEG C, and ultrasonic time 20min extracts 2 times.
In above-mentioned steps, note lucifuge, in operating process, action should be wanted rapidly, after EO, to cover as early as possible bottle cap, avoids sample liquid too much to contact with air and oxidized.
Positively effect of the present invention is as follows:
The method of extracting carotenoid from incense with golden color willow of the present invention has advantages of convenient and swift and simple to operate, low for equipment requirements, and uses the extraction yield of method extraction carotenoid of the present invention high.
Embodiment
The following examples are to describe in further detail of the present invention.
Embodiment 1
(1) first gather fresh incense with golden color willow leaf sheet, then put into Freeze Drying Equipment freeze-drying 30h;
(2) the incense with golden color willow leaf sheet after freeze-drying is pulverized, crossed 40 mesh sieves;
(3) then toward pulverize after blade in add acetone and sherwood oil mixed extract, solid-liquid ratio is 1: 15;
(4) carry out ultrasonic assisted extraction, ultrasonic power is 40W again, and ultrasonic temperature is 20 DEG C, and ultrasonic time 50min extracts 4 times;
(5) extract after, carry out centrifugal, at 4 DEG C, under rotating speed 8000rpm, extract 10min, draw supernatant liquor, can obtain the solution that contains carotenoid.
In step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 1: 3.
Embodiment 2
(1) first gather fresh incense with golden color willow leaf sheet, then put into Freeze Drying Equipment freeze-drying 60h;
(2) the incense with golden color willow leaf sheet after freeze-drying is pulverized, crossed 40 mesh sieves;
(3) then toward pulverize after blade in add acetone and sherwood oil mixed extract, solid-liquid ratio is 1: 40;
(4) carry out ultrasonic assisted extraction, ultrasonic power is 90W again, and ultrasonic temperature is 70 DEG C, and ultrasonic time 50min extracts 1 time;
(5) extract after, carry out centrifugal, at 4 DEG C, under rotating speed 8000rpm, extract 10min, draw supernatant liquor, can obtain the solution that contains carotenoid.
In step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 9: 3.
Embodiment 3
(1) first gather fresh incense with golden color willow leaf sheet, then put into Freeze Drying Equipment freeze-drying 48h;
(2) the incense with golden color willow leaf sheet after freeze-drying is pulverized, crossed 40 mesh sieves;
(3) then toward pulverize after blade in add acetone and sherwood oil mixed extract, solid-liquid ratio is 1: 36;
(4) carry out ultrasonic assisted extraction, ultrasonic power is 50W again, and ultrasonic temperature is 63 DEG C, and ultrasonic time 20min extracts 2 times;
(5) extract after, carry out centrifugal, at 4 DEG C, under rotating speed 8000rpm, extract 10min, draw supernatant liquor, can obtain the solution that contains carotenoid.
In step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 6: 3.
The mensuration of carotenoid content
The solution that contains carotenoid prepared by embodiment of the present invention 1-3 carries out the mensuration of carotenoid content.
Measuring method:
Draw supernatant liquor 2mL, revolve and steam to dry, use 2mL sherwood oil to redissolve quantitatively, shake up and make it whole dissolvings; Draw 0.5mL sherwood oil again and be diluted to 5mL, measure absorbancy with spectrophotometer at 445nm place, calculate yield according to formula (1), test repeats 2 times.
X＝(1000EV)/(A×W) (1)
In formula: X-total carotinoid content (mg kg
-1); The absorbance that E-records; The volume (mL) of the last constant volume of V-sample; The alleged example weight of W-(g); The average optical extinction coefficient of A-carotenoid is 2500.
Experimental result is as shown in table 1:
Table 1
| Sample | Extract yield (mg kg -1DW) |
| Embodiment 1 | 19.32 |
| Embodiment 2 | 18.65 |
| Embodiment 3 | 21.73 |
Although illustrated and described embodiments of the invention, for the ordinary skill in the art, be appreciated that without departing from the principles and spirit of the present invention and can carry out multiple variation, amendment, replacement and modification to these embodiment, scope of the present invention is limited by claims and equivalent thereof.
Claims (9)
1. a method of extracting carotenoid from incense with golden color willow, is characterized in that: the concrete steps of described method are as follows:
(1) first gather fresh incense with golden color willow leaf sheet, then put into Freeze Drying Equipment freeze-drying 30-60h;
(2) the incense with golden color willow leaf sheet after freeze-drying is pulverized, crossed 40 mesh sieves;
(3) then toward pulverize after blade in add acetone and sherwood oil mixed extract, solid-liquid ratio is 1: 15-40;
(4) carry out ultrasonic assisted extraction, ultrasonic power is 40-90W again, and ultrasonic temperature is 20-70 DEG C, and ultrasonic time 5-50min extracts 1-4 time;
(5) extract after, carry out centrifugal, draw supernatant liquor, can obtain the solution that contains carotenoid.
2. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (1), put into Freeze Drying Equipment freeze-drying 48h.
3. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 1-9: 3.
4. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), in acetone and sherwood oil mixed extract, the weight ratio of acetone and sherwood oil is 6: 3.
5. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), solid-liquid ratio is 1: 36.
6. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), ultrasonic power is 50W.
7. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), ultrasonic temperature is 63 DEG C.
8. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), and ultrasonic time 20min.
9. the method for extracting carotenoid from incense with golden color willow as claimed in claim 1, is characterized in that: in step (3), extract 2 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410239312.8A CN104003919B (en) | 2014-06-03 | 2014-06-03 | A kind of method extracting carotenoid from incense with golden color willow |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410239312.8A CN104003919B (en) | 2014-06-03 | 2014-06-03 | A kind of method extracting carotenoid from incense with golden color willow |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104003919A true CN104003919A (en) | 2014-08-27 |
| CN104003919B CN104003919B (en) | 2016-02-17 |
Family
ID=51364854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410239312.8A Expired - Fee Related CN104003919B (en) | 2014-06-03 | 2014-06-03 | A kind of method extracting carotenoid from incense with golden color willow |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104003919B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0670306A1 (en) * | 1994-03-02 | 1995-09-06 | Nippon Oil Co. Ltd. | Process for extracting carotenoids |
| CN1431196A (en) * | 2003-01-08 | 2003-07-23 | 张声华 | Carotenoid possessing anticancer function and its preparing method |
| CN101054357A (en) * | 2007-05-18 | 2007-10-17 | 武汉大学 | Method for extracting total carotinoid from turmeric |
| CN101973920A (en) * | 2010-10-18 | 2011-02-16 | 天津商业大学 | Method for extracting carotenoid from physalis persistent calyx |
| WO2011064710A1 (en) * | 2009-11-30 | 2011-06-03 | University Of Kwazulu-Natal | In vitro anti-sickling activity of betulinic acid, oleanolic acid and their derivatives |
| CN102960248A (en) * | 2012-11-28 | 2013-03-13 | 巴中市光雾山植物研究所 | Melaleuca bracteata tissue culture and rapid propagation process |
-
2014
- 2014-06-03 CN CN201410239312.8A patent/CN104003919B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0670306A1 (en) * | 1994-03-02 | 1995-09-06 | Nippon Oil Co. Ltd. | Process for extracting carotenoids |
| CN1431196A (en) * | 2003-01-08 | 2003-07-23 | 张声华 | Carotenoid possessing anticancer function and its preparing method |
| CN101054357A (en) * | 2007-05-18 | 2007-10-17 | 武汉大学 | Method for extracting total carotinoid from turmeric |
| WO2011064710A1 (en) * | 2009-11-30 | 2011-06-03 | University Of Kwazulu-Natal | In vitro anti-sickling activity of betulinic acid, oleanolic acid and their derivatives |
| CN101973920A (en) * | 2010-10-18 | 2011-02-16 | 天津商业大学 | Method for extracting carotenoid from physalis persistent calyx |
| CN102960248A (en) * | 2012-11-28 | 2013-03-13 | 巴中市光雾山植物研究所 | Melaleuca bracteata tissue culture and rapid propagation process |
Non-Patent Citations (1)
| Title |
|---|
| 钟昌勇等: "黄金香柳枝叶挥发性精油提取与分析", 《香料香精化妆品》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104003919B (en) | 2016-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6545913B2 (en) | Air cleaner composition and method of preparing the same | |
| CN102028646A (en) | Thyme perfume | |
| CN106221939A (en) | A kind of plants essential oil with antibacterial effect and preparation method and application | |
| Arora et al. | Antimicrobial & antioxidant activity of orange pulp and peel | |
| CN103705620B (en) | The preparation method of indissoluble combined state polyphenol in a kind of Fructus Litchi | |
| CN102241786A (en) | Preparation method and application of selenium enriched puerarin polysaccharide | |
| CN104610203A (en) | Method for extracting vitamin C from shine skin papayas | |
| CN103875722A (en) | Botanical fungiticide prepared by carrying out combined extraction on Chinese herbal medicines | |
| Yu et al. | Function of leafy sepals in Paris polyphylla: photosynthate allocation and partitioning to the fruit and rhizome | |
| CN104815191A (en) | Processing method of rhizoma gastrodiae decoction pieces | |
| Teoh et al. | Antioxidant properties and yield of wood ear mushroom, Auricularia polytricha (Agaricomycetes), cultivated on rubberwood sawdust | |
| CN103583574B (en) | Plant source smoke water and its preparation method and use in promotion of red sage root active-substance accumulation | |
| Verma et al. | Nutritive assessment of different plant parts of Carica papaya Linn of Jabalpur region | |
| CN103536495B (en) | A kind of preparation method of grape pip procyanidin sunscreen cream | |
| CN103333149A (en) | Extraction method of date peel cyanidin | |
| CN107821455B (en) | Traditional Chinese medicine preparation for health care and quality improvement of tobacco and use method thereof | |
| CN104003919A (en) | Method for extracting carotenoid from Melaleuca bracteata | |
| Orphanides et al. | Recovery of essential oils from carobs through various extraction methods | |
| Abeywardhana et al. | Determination of optimum maturity stage for Ocimum sanctum L. grown under different growing systems in terms of therapeutically active secondary metabolites | |
| Sharma et al. | Effect of cryogenic grinding on oil yield, phenolics and antioxidant properties of ajwain (Trachyspermum ammi L.) | |
| Alsiede et al. | Phytochemical screening, total phenolics content and antioxidants activity of Cassia Singueana | |
| Asha et al. | Evaluation of phytochemicals and antioxidant activities of Remusatia vivipara (Roxb.) Schott., an edible genus of Araceae | |
| Latif et al. | Enhancing polyphenol extraction from white button mushrooms using microwave-assisted extraction: A response surface methodology optimization approach | |
| Chandra | Qualitative and Quantitative Analysis of Phytochemical Variation in G. corticata and K. alvarezii | |
| CN105924376A (en) | Method for extracting beta-carotene from persimmons |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190314 Address after: 363000 West of Danxia Road, Xiangcheng District, Zhangzhou City, Fujian Province, and North of Jianyuan Road, Lidu Garden, Shipu Road, No. 6, No. 219 Patentee after: Zhangzhou Senjing Biotechnology Co., Ltd. Address before: Room 805, Building 4, Jianyuan South Garden, 56 Jianyuan Road, Xiancheng District, Zhangzhou City, Fujian Province Patentee before: Lin Xiaohong |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160217 Termination date: 20210603 |