CN101973920A - Method for extracting carotenoid from physalis persistent calyx - Google Patents
Method for extracting carotenoid from physalis persistent calyx Download PDFInfo
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- CN101973920A CN101973920A CN2010105108758A CN201010510875A CN101973920A CN 101973920 A CN101973920 A CN 101973920A CN 2010105108758 A CN2010105108758 A CN 2010105108758A CN 201010510875 A CN201010510875 A CN 201010510875A CN 101973920 A CN101973920 A CN 101973920A
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Abstract
The invention discloses a method for extracting carotenoid from physalis persistent calyx, aiming at providing a method for extracting carotenoid from physalis persistent calyx with the advantages of no requirement on organic solvents, short production cycle and high extraction ratio by adopting a supercritical CO2 extraction method. The method comprises the following steps of: washing, drying and crushing the physalis persistent calyx and sieving by a 20 to 200 mesh sieve; extracting the crushed and sieved physalis persistent calyx powder to obtain the carotenoid by adopting the supercritical CO2 extraction method, wherein the temperature of supercritical CO2 extraction is 35 to 65DEG C; the extraction pressure is 15 to 45MPa; and the extraction time is 30 to 200min. The method adopts the supercritical CO2 extraction method and has the advantages of friendly environment, no requirement on the organic solvents, simple and convenient extraction and separation process, no pollution, short production cycle and good quality of the prepared pigment, and the extraction ratio can reach 15.96mg/g by determining the process conditions of the temperature, the pressure, and the like in the extraction course.
Description
Technical field
The present invention relates to a kind of extracting method that from the place calyx of the medicine food dual purpose plant wintercherry that is rich in carotenoid, extracts carotenoid with antitumous effect.
Background technology
Wintercherry is a Solanaceae Physalis per nnial herb, is also referred to as red Miss, Calyx seu Fructus physalis, Herb of Peruvian Gruondcherry, Lip river cornea, Physalis alkekengi L. var. francheti (Mast.) Makino etc.Abundant at China's wild resource, except that Tibet, all there is distribution other various places, and are in the majority with the north.The place calyx that it expands can be used as medicine, have refrigerant, reduce phlegm, the effect of antibechic, diuresis.(Hui Baidi, Wang Yajing, Pei Lingpeng etc. such as Hui Baidi, carotenoid compositional analysis in wintercherry berry and the place calyx, Chinese Pharmaceutical Journal, 2006,41, studies show that contain abundant carotenoid in the Calyx Physalis 583-584.) that it mainly consists of zeaxanthin and xenthophylls etc.Simultaneously, the multidigit scholar's research finds that zeaxanthin and xenthophylls not only have pigmentation, also have following physiological function: 1, anti-oxidant function both at home and abroad; 2, protection eyes; 3, prevention cataract; 4, preventing cancer; 5, preventing cardiovascular disease.Therefore, natural carotenoid has very high nutrition and edibleness.
At present, the extracting method of carotenoid is to adopt the organic solvent extracting access method.When adopting the organic solvent extracting access method to extract carotenoid, make that easily the quality product of gained pigment is relatively poor, also cause the remaining of organic solvent, extraction yield is also lower simultaneously, as (Hui Baidi such as Hui Baidi, Wang Yajing, Pei Lingpeng etc., the carotenoid compositional analysis in wintercherry berry and the place calyx, Chinese Pharmaceutical Journal, 2006,41,583-584.) adopting the extraction yield of organic solvent extraction method only is 8.19mg/g.
Summary of the invention
The present invention is in order to overcome weak point of the prior art, a kind of employing supercritical CO to be provided
2Extraction process, environmental friendliness does not need organic reagent, and extraction and separation process is easy, and is pollution-free, with short production cycle, the method for extracting carotenoid from Calyx Physalis that extraction yield is high.
The present invention is achieved through the following technical solutions:
A kind of method of extracting carotenoid from Calyx Physalis is characterized in that, comprises the steps:
(1) to Calyx Physalis clean, dry, pulverize, the 20-200 order sieves;
(2) the Calyx Physalis powder behind the crushing screening is adopted supercritical CO
2The extraction process supercritical extraction obtains carotenoid, wherein, and supercritical CO
2The temperature of extraction is 35-65 ℃, and extracting pressure is 15-45MPa, extraction time 30-200min.
The drying temperature of Calyx Physalis is 50 ℃ in the step (1), and be 24 hours time of drying.
Supercritical CO
2In the extraction process, Calyx Physalis particles of powder degree is the 100-200 order, supercritical CO
2The temperature of extraction is 50 ℃, and extracting pressure is 40MPa, extraction time 200min.
The present invention has following technique effect:
The extracting method of carotenoid of the present invention adopts supercritical CO
2Extraction process, its principle are to utilize the supercritical flow volume density to change along with the variation of its pressure and temperature, and then influence its dissolving power, reach the purpose of dissolving extract.Therefore, adopt supercritical CO
2Fluid extracts the carotenoid environmental friendliness from Calyx Physalis, do not need organic reagent, and extraction and separation process is easy, pollution-free, with short production cycle, the quality of gained pigment is good, and, make extraction yield up to 15.96mg/g by to the determining of processing condition such as temperature, pressure in the leaching process
Description of drawings
The UV scanning figure of the carotenoid that Fig. 1 obtains for the embodiment of the invention 1.
The influence of the extract stability that Fig. 2 obtains embodiment 1 for illumination condition;
The influence of the extract stability that Fig. 3 obtains embodiment 1 for metal ion;
The influence of the extract stability that Fig. 4 obtains embodiment 1 for temperature;
Fig. 5 is H
2O
2The influence of the extract stability that concentration obtains embodiment 1;
The influence of the extract stability that Fig. 6 obtains embodiment 1 for foodstuff additive.
Embodiment
Supercritical CO of the present invention
2Extraction plant is that the model that American I SCO company produces is the extraction plant of SFX220, also can adopt other various types of supercritical COs
2Extraction plant.
Below the model of producing with American I SCO company be that the extraction plant of SFX220 is an experimental installation, and the present invention is described in detail in conjunction with specific embodiments.
Embodiment 1
Calyx Physalis is cleaned, and oven dry is 24 hours under 50 ℃ of conditions, pulverizes through pulverizer, and the 100-200 order sieves, and obtains the Calyx Physalis powder.
The Calyx Physalis powder is filled extraction kettle, place receiving trap.Connect power supply, open whole extraction plant by Controlling System.The refrigerator temperature is made as about 5 ℃, sets the temperature required and extracting pressure of extraction by Controlling System.From steel cylinder, flow out CO
2, be liquefied as liquid through refrigerator, reach required pressure through pump pressurization, after preheater is heated to preset temperature, enters in the extraction kettle by the bottom and to extract, be in the CO of supercritical state this moment
2Just carotenoid is extracted from Calyx Physalis.Be dissolved with the CO of carotenoid
2Enter the reception separator through reducing valve, along with pressure descends, CO
2Dissolving power descend, the bottom of separator is separated out and be deposited in to solute, opens valve and can make CO
2Circulation is used.Through certain extraction time, will receive the separator internal pressure and reduce to normal pressure, obtain extract, promptly obtain carotenoid.
Extraction temperature is 50 ℃ in the said process, and extracting pressure is 40MPa, and the extraction time is 200min.With extract dehydrated alcohol constant volume, and be blank with the dehydrated alcohol, with TU-1810 type ultraviolet-visible pectrophotometer it is scanned in wavelength 400-500nm scope, obtain the UV scanning figure of carotenoid, as shown in Figure 1, at wavelength 400-500nm strong absorption peak is arranged as can be known, its maximum absorption wavelength is 450nm.According to absorbance in the maximum absorption wave strong point, calculate by youth's primary-Beer's law, the extraction yield that obtains present embodiment is 15.96mg/g.
By measuring illumination condition, temperature, metal ion, H
2O
2Concentration and foodstuff additive are to the influence of extract stability, and the result is shown in Fig. 2-6, and the extract that embodiment 1 obtains has higher heat-resistant stability, but illumination has destructiveness to it; The destructiveness difference of different metal ion pair carotene, Fe
3+, Al
3+Destructiveness is bigger, Cu
2+, Zn
2+, Ca
2+, Fe
2+Less to stability influence; Oxygenant and common food additive sucrose, glucose and salt are not remarkable to the pigment stability influence, (Cheng Yingkun such as this and Cheng Yingkun, Li Lei, the wangdan plum, Teng Lirong, the extraction process of Calyx Physalis pigment and Study on Stability, foodstuffs industry science and technology, 2006,12, report 58-64) is unanimity as a result.Simultaneously, analyze by extract being carried out HPLC that wherein zeaxanthin content is the highest as can be known is about 75%, secondly be about 16% and 8% respectively for beta-cryptoxanthin and xenthophylls.
Embodiment 2
Experimental installation is identical with embodiment 1 with method, and wherein, extraction temperature is 60 ℃, and extracting pressure is 30MPa, and Calyx Physalis powder particle particle diameter is the 100-200 order, extraction time 120min.Through ultra-violet analysis, the present embodiment extraction yield is about 11.32mg/g.
Embodiment 3
Experimental installation is identical with embodiment 1 with method, supercritical CO
2The temperature of extraction is 50 ℃, and extracting pressure is 30MPa, extraction time 120min, and Calyx Physalis powder particle particle diameter is the 60-80 order.Through ultra-violet analysis, the present embodiment extraction yield is about 9.20mg/g.
Claims (3)
1. a method of extracting carotenoid from Calyx Physalis is characterized in that, comprises the steps:
(1) to Calyx Physalis clean, dry, pulverize, the 20-200 order sieves;
(2) the Calyx Physalis powder behind the crushing screening is adopted supercritical CO
2The extraction process supercritical extraction obtains carotenoid, wherein, and supercritical CO
2The temperature of extraction is 35-65 ℃, and extracting pressure is 15-45MPa, extraction time 30-200min.
2. the method for extracting carotenoid from Calyx Physalis according to claim 1 is characterized in that the drying temperature of Calyx Physalis is 50 ℃ in the step (1), and be 24 hours time of drying.
3. the method for extracting carotenoid from Calyx Physalis according to claim 1 is characterized in that supercritical CO
2In the extraction process, Calyx Physalis particles of powder degree is the 100-200 order, supercritical CO
2The temperature of extraction is 50 ℃, and extracting pressure is 40MPa, extraction time 200min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105108758A CN101973920B (en) | 2010-10-18 | 2010-10-18 | Method for extracting carotenoid from physalis persistent calyx |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219721A (en) * | 2011-04-02 | 2011-10-19 | 秦皇岛大惠生物技术有限公司 | Extraction method for concentrate containing beta-cryptoxanthin, concentrate obtained by the method, and usage of the obtained concentrate |
| CN104003919A (en) * | 2014-06-03 | 2014-08-27 | 林晓红 | Method for extracting carotenoid from Melaleuca bracteata |
| CN104337927A (en) * | 2013-07-31 | 2015-02-11 | 中国科学院大连化学物理研究所 | Calyx seu fructus physalis extractive, medicine composition and/or functional food |
| CN105038300A (en) * | 2015-07-10 | 2015-11-11 | 珲春界三国农业有限公司 | Method for extracting yellow pigment from physalis alkekengi fruits |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367208A (en) * | 2002-03-04 | 2002-09-04 | 大庆石油管理局 | Process for extracting carotenol by using supercritical CO2 extraction |
-
2010
- 2010-10-18 CN CN2010105108758A patent/CN101973920B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367208A (en) * | 2002-03-04 | 2002-09-04 | 大庆石油管理局 | Process for extracting carotenol by using supercritical CO2 extraction |
Non-Patent Citations (4)
| Title |
|---|
| 《食品科学》 20081231 惠伯棣 超临界CO2 中全反式beta,beta - 和psi,psi - 胡萝卜素的电子吸收光谱位移研究 125-128 1-3 第29卷, 第10期 * |
| 惠伯棣,等: "酸浆浆果和宿萼中的类胡萝卜素组成分析", 《中国药学杂志》 * |
| 惠伯棣: "超临界CO2 中全反式β,β - 和ψ,ψ - 胡萝卜素的电子吸收光谱位移研究", 《食品科学》 * |
| 程瑛琨，等: "酸浆宿萼色素的提取工艺及稳定性的研究", 《食品工业科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219721A (en) * | 2011-04-02 | 2011-10-19 | 秦皇岛大惠生物技术有限公司 | Extraction method for concentrate containing beta-cryptoxanthin, concentrate obtained by the method, and usage of the obtained concentrate |
| CN102219721B (en) * | 2011-04-02 | 2013-08-07 | 秦皇岛大惠生物技术有限公司 | Extraction method for concentrate containing beta-cryptoxanthin, concentrate obtained by the method, and usage of the obtained concentrate |
| CN104337927A (en) * | 2013-07-31 | 2015-02-11 | 中国科学院大连化学物理研究所 | Calyx seu fructus physalis extractive, medicine composition and/or functional food |
| CN104003919A (en) * | 2014-06-03 | 2014-08-27 | 林晓红 | Method for extracting carotenoid from Melaleuca bracteata |
| CN104003919B (en) * | 2014-06-03 | 2016-02-17 | 林晓红 | A kind of method extracting carotenoid from incense with golden color willow |
| CN105038300A (en) * | 2015-07-10 | 2015-11-11 | 珲春界三国农业有限公司 | Method for extracting yellow pigment from physalis alkekengi fruits |
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| CN101973920B (en) | 2013-08-07 |
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