CN103989826A - Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof - Google Patents
Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof Download PDFInfo
- Publication number
- CN103989826A CN103989826A CN201410232557.8A CN201410232557A CN103989826A CN 103989826 A CN103989826 A CN 103989826A CN 201410232557 A CN201410232557 A CN 201410232557A CN 103989826 A CN103989826 A CN 103989826A
- Authority
- CN
- China
- Prior art keywords
- radix sophorae
- sophorae flavescentis
- body surface
- animal body
- stellera chamaejasme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and a preparation method thereof. The preparation method comprises the steps of extracting a Chinese Stellera Root paste extract, extracting sophora flavescens biological total alkali, and fixing the volume of 20 g of the Chinese Stellera Root paste extract, the sophora flavescens biological total alkali, a surfactant (SDS) and a cosurfactant (ethanol) to 100 ml by using distilled water according to a ratio of 10:6:3:1. The compound microemulsion animal body surface insecticide disclosed by the invention has an obvious effect on such ectozoa as mite, lice, tick of animals, and has the advantages of no animal irritation, low toxicity and long-lasting effect.
Description
Technical field
The invention belongs to drug manufacture field, the animal body surface insecticide that more precisely belongs to the compound preparation of plant extract is produced.
Background technology
Now insecticide major part is chemical antibiosis class medicine, and the of short duration even polypide of insecticidal effect develops immunity to drugs, and after animal-use drug, untoward reaction is obvious etc. in addition.And Stellera chamaejasme L., Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide insecticidal effect are more obvious, and to animal nonirritant, low toxicity, long-acting.
In prior art, there is the extracting method of multiple Stellera chamaejasme L. and Radix Sophorae Flavescentis, extracting method difference, the Stellera chamaejasme L. obtaining and the component of Radix Sophorae Flavescentis and molecular structure have larger difference, and its biologic activity also will be thereupon different.
Summary of the invention
In order to overcome above-mentioned prior art deficiency, the invention provides Stellera chamaejasme L., Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide and preparation method thereof, used natural plant extract, noresidue, low toxicity, and successful also to animal, non-stimulated to go back insecticidal effect long-acting.
An object of the present invention is to provide a kind of Stellera chamaejasme L., Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface method for producing insecticide, its step comprises:
1. Stellera chamaejasme L. is pulverized for 20-30 order, with 6 times of amount 95% ethanol, at 75 DEG C of temperature, soaked 5 days, after filtering, concentrated by rotary evaporation is paste;
2. Radix Sophorae Flavescentis is pulverized for 10-20 order, add 10 times of amount 65% ethanol, soak percolation 24h, flow velocity is 5ml/min, is adjusted to 6.5-7 by extracting the pH value of solution, removes lipotropy impurity by petroleum ether and ethyl acetate, acid solution cold preservation 2～3d, precipitate non-alkali composition, after filtration, make alkaloid free sour water alkalization, then obtain Radix Sophorae Flavescentis biology total alkali with chloroform extraction;
3. Stellera chamaejasme L. mastic extract, Radix Sophorae Flavescentis biology total alkali, surfactant, cosurfactant are dissolved in distilled water.
Especially, Stellera chamaejasme L. mastic extract, by Radix Sophorae Flavescentis total alkaloids, surfactant, cosurfactant 10: 6: 3 in mass ratio: after 1 (20g altogether) mixes, be dissolved in distilled water, make 100ml solution.
Especially, described surfactant is SDS, and cosurfactant is ethanol.
Another object of the present invention has been to provide a kind of composite micro-emulsion agent animal body surface insecticide being made by said method.
Compared with prior art, the invention has the beneficial effects as follows:
1, raw material is natural plant extract, non-stimulated, noresidue.
2, investment of production is few, controls simply, is easy to grasp and promote.
3, product is taking water as medium, less containing organic solvent, thereby do not fire not quick-fried, production operation, storing is safe, low in the pollution of the environment, saves a large amount of organic solvents;
4, effect more obviously and also low toxicity also long-acting;
5, microemulsion belongs to thermodynamic stable system, has advantages of that ageing stability is good;
6, there is not drug resistance, be not residually difficult for causing other pathological changes;
7, principal agent dispersion is high, reaches miniaturization degree, and principal agent particle is generally 0.1～0.01 micron, and outward appearance is similar to transparent or sub-translucent liquid; Good dispersion in water is strong to target body permeability, adhesive force good.
Detailed description of the invention
Embodiment 1
The extraction of Stellera chamaejasme L.
1. be 20-30 order by root of Stellera chamaejasme drying and crushing;
2. in the water-bath of 75 DEG C, soak 5 days with 95% ethanol of 6 times of amounts;
3. with after buchner funnel sucking filtration, be paste with revolving steaming instrument concentrated by rotary evaporation.
The extraction of Radix Sophorae Flavescentis
1., after Radix Sophorae Flavescentis being dried, pulverize the order into 10-20;
2. with 10 times of amount 65% ethanol, soak diafiltration 24 hours, flow velocity is 5ml/min;
3. thick alkali is adjusted to faintly acid, removes lipotropy impurity by petroleum ether and ethyl acetate;
4. acid solution cold preservation 2～3d, precipitates non-alkali composition, after filtration, makes alkaloid free sour water alkalization, then obtains purer total alkaloids with chloroform extraction.
By Stellera chamaejasme L. mastic extract, by Radix Sophorae Flavescentis total alkaloids, surfactant (SDS), cosurfactant (ethanol) by 10: 6: 3: 1 (altogether 20g) standardize solution is 100ml distilled water.
Embodiment 2
The LD50 of plant source external animal body surface insecticide the results are shown in Table 1:
Table 1
The oral median lethal dose(LD 50) of white mice (LD50) that can prove Stellera chamaejasme L., Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide by above-mentioned test is 327 mgs/kg, 95% credibility interval is 287-361 mg/kg, belongs to low toxicity product by national toxicity criterion.
Repeatedly skin irritation test the results are shown in Table 2:
Table 2
Can prove that by above-mentioned test Stellera chamaejasme L. that we develop, Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide are to skin nonirritant.
To the flock of sheep experimental result that infects itch mite in table 3:
Table 3
The successful that can prove Stellera chamaejasme L. that we develop, Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide by above-mentioned test is more long-acting.
Show from above experiment, Stellera chamaejasme L., Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide prepared by the present invention are more obvious to ectoparasite disease effects such as the various demodicid mites of animal, louse, Tickss, and to animal nonirritant, low toxicity, long-acting.
Microemulsion that Different Extraction Method is prepared and present common drug, carry out therapeutic effect comparison to the ear demodicid mite of laboratory animal White Rabbit (10 every group), and spray medicine and after one week, observe the number of elements of relatively having treated as follows, in table 4:
Table 4:
Stellera chamaejasme L. extraction process different multiples different concentration ethanol extraction comparison, and the extraction of different temperatures and extraction time contrast (5 every group sheep demodicid mites alive, test), the results are shown in Table 5:
Table 5
Adopt equally in the above conditions soak time contrast in 3,5,7 days, found to soak 5 days insecticidal effect the bests.
Claims (3)
1. Stellera chamaejasme L., a Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface insecticide, is characterized by Stellera chamaejasme L. mastic extract, Radix Sophorae Flavescentis total alkaloids, surfactant, cosurfactant are total to 20g by 10: 6: 3: 1 use distilled water standardize solution is that 100ml forms.
2. according to a kind of Stellera chamaejasme L. of claim 1, Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface method for producing insecticide, its step comprises:
1) Stellera chamaejasme L. being pulverized is 20～30 orders, with 6 times of amount 95% ethanol, at 75 DEG C of temperature, soaks 5 days, and after filtering, concentrated by rotary evaporation is paste;
2) Radix Sophorae Flavescentis being pulverized is 10～20 orders, add 10 times of amount 65% ethanol, soak 24h percolation, flow velocity is 5ml/min, is adjusted to 6.5～7 by extracting the pH value of solution, removes lipotropy impurity by petroleum ether and ethyl acetate, acid solution cold preservation 2～3d, precipitate non-alkali composition, after filtration, make alkaloid free sour water alkalization, then obtain Radix Sophorae Flavescentis biology total alkali with chloroform extraction;
3) Stellera chamaejasme L. mastic extract, Radix Sophorae Flavescentis biology total alkali, surfactant, cosurfactant are dissolved in distilled water in proportion.
3. according to a kind of Stellera chamaejasme L. of claim 2, Radix Sophorae Flavescentis composite micro-emulsion agent animal body surface method for producing insecticide, wherein said surfactant is that SDS, cosurfactant are ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410232557.8A CN103989826A (en) | 2014-05-23 | 2014-05-23 | Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410232557.8A CN103989826A (en) | 2014-05-23 | 2014-05-23 | Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103989826A true CN103989826A (en) | 2014-08-20 |
Family
ID=51304326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410232557.8A Pending CN103989826A (en) | 2014-05-23 | 2014-05-23 | Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103989826A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684612A (en) * | 2019-10-31 | 2020-01-14 | 呼和浩特市亿羊达生物科技有限责任公司 | Stellera chamaejasme sheep oil soap for preventing and treating animal dermatophytosis and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548679A (en) * | 2009-05-12 | 2009-10-07 | 沈阳药科大学 | Matrine type alkaloid microemulsion and preparation method thereof |
| CN103621504A (en) * | 2013-11-26 | 2014-03-12 | 内蒙古自治区农牧业科学院 | Stellera chamaejasme microemulsion and preparation method thereof |
-
2014
- 2014-05-23 CN CN201410232557.8A patent/CN103989826A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548679A (en) * | 2009-05-12 | 2009-10-07 | 沈阳药科大学 | Matrine type alkaloid microemulsion and preparation method thereof |
| CN103621504A (en) * | 2013-11-26 | 2014-03-12 | 内蒙古自治区农牧业科学院 | Stellera chamaejasme microemulsion and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 江茂生等: "植物源农用杀菌剂的研究进展", 《福建省科协第五届学术年会提高海峡西岸经济区农业综合生产能力分会场论文集》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684612A (en) * | 2019-10-31 | 2020-01-14 | 呼和浩特市亿羊达生物科技有限责任公司 | Stellera chamaejasme sheep oil soap for preventing and treating animal dermatophytosis and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101406204B (en) | Plant powder pesticide and preparation method thereof | |
| CN106420465A (en) | Soothing perfume | |
| CN103548852B (en) | A kind of containing alkaloidal insecticides and uses thereof | |
| CN106007980A (en) | Eaglewood edgeworthia chrysantha inducer and Aquilaria agallocha production method | |
| CN101518267B (en) | Aqueous pesticide solution compounded by silver chain extract and tea saponin and method for preparing same | |
| CN103989826A (en) | Chinese Stellera Root-sophora flavescens compound microemulsion animal body surface insecticide and preparation method thereof | |
| CN101209061B (en) | Preparation method of botanical insecticide | |
| CN102132707B (en) | Extraction method capable of improving antibacterial activity of extract of leaves of fagaceae plant | |
| CN103598203B (en) | Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech | |
| CN102657712A (en) | Preparation method for adonis coerulea maxim extract and application thereof | |
| CN101961399A (en) | Preparation method of insecticide for aquatic animals | |
| CN103222469A (en) | Method for producing sophocarpidine water aqua by using enzymolysis method | |
| CN103638052A (en) | Polyrhachis dives extractive, and preparation and medical purpose thereof | |
| CN104068031B (en) | Hamaudol is applied preparing in pesticide | |
| Harpreet et al. | Phytochemical analysis of Aesculus indica seeds extracts | |
| CN104586837B (en) | Purposes of the gray wool Cipadessa baecifera Miq A and the like in anti-depression drug is prepared | |
| Ghramh et al. | Phyto-Synthesis, Characterization and Biological Applications of Gold Nanoparticles (AuNPs) Using Toxicosis Mediated Plant Nicotiana glauca Graham | |
| CN103044500B (en) | A kind of organic acid preparation and antibacterial applications | |
| CN102127089A (en) | Method for extracting camptothecin | |
| CN102224829A (en) | Albizzia julibrissin durazzini extract and method for preparing water agent with extract to control melon diseases | |
| CN102335234A (en) | Alkaloid extract of trembling poplar as well as extraction method and application thereof | |
| Tiwari et al. | Phytochemical investigation of Caltropis procera flower extract | |
| CN108135899A (en) | Anti-fungal infection agent | |
| CN105638691B (en) | Ring calyculus tongue alcohol -3- caffeic acid esters are applied in insecticide is prepared | |
| CN102626460A (en) | Preparation method of Zanthoxylum dissitum Hemsl skin polysaccharide and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140820 |
|
| RJ01 | Rejection of invention patent application after publication |