CN103981099A - Culture medium and culture method for cultivating Navicula seminulum by utilizing phosphate fertilizer plant wastewater - Google Patents
Culture medium and culture method for cultivating Navicula seminulum by utilizing phosphate fertilizer plant wastewater Download PDFInfo
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- CN103981099A CN103981099A CN201410247884.0A CN201410247884A CN103981099A CN 103981099 A CN103981099 A CN 103981099A CN 201410247884 A CN201410247884 A CN 201410247884A CN 103981099 A CN103981099 A CN 103981099A
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- phosphate fertilizer
- algae
- milliliters
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- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- ABTOQLMXBSRXSM-UHFFFAOYSA-N silicon tetrafluoride Chemical compound F[Si](F)(F)F ABTOQLMXBSRXSM-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a culture medium and culture method for cultivating Navicula seminulum by utilizing phosphate fertilizer plant wastewater. The method can treat phosphate fertilizer plant wastewater at low cost, utilize waste, change wastes into valuable substances and obtain the Navicula seminulum with important bait value, thereby implementing waste utilization and changing wastes into valuable substances. In the traditional process, the Navicula seminulum is cultured in a plastic barrel or plastic film bag by using an f/2 culture medium formula; and thus, the traditional process has the defects of low growth speed, high tendency to pollution, high cost, low benefits, labor consuming in operation, and high workload. In the invention, an improved rectangular runway tank is utilized for culture; phosphate fertilizer plant wastewater, sodium silicate, sodium platinate, chicken manure extract, sodium carboxymethyl cellulose, propylene glycol alginate, sodium D-isoascorbate, M-F trace element solution, ferric citrate and other components are added into the culture medium, and the organic fertilizers and inorganic fertilizers are mixed; and thus, the method has the advantages of more comprehensive and balanced nutrients, low tendency to pollution, low cost and high benefits, is convenient to operate, greatly enhances the growth speed of the Navicula seminulum, and enhances the yield by 360%.
Description
Technical field
The invention belongs to aquaculture field, relate in particular to a kind of substratum and cultural method that utilizes phosphate fertilizer plant's waste water to cultivate half-naked boat-shaped algae.
Background technology
The caused pollution that develops rapidly of Phosphate Fertilizer Industry is in recent years on the rise, and has caused people's extensive concern.Data demonstration, 1 ton of fused(calcium magnesium)phosphate of every production will discharge 40 tons of waste water.The waste water fluoro-containing concentration of discharging in phophatic fertilizer production process is up to 120mg/L.Fluorine is a kind of to human body and animal body and the harmful element of environment, scientific research personnel investigates demonstration, the workman of phosphate fertilizer plant, family members and around 300 meters of dental fluorosis recall rates of take interior resident are 8.4%, children, juvenile Geng Gao, be 36%, fluoride pollution also can cause that bone density increases and bone trabecula increases slightly, grid increase, structural fuzzy etc. are the skeletal fluorosis of symptom, and blood of human body and nerve are also had to detrimentally affect.Calcium superphosphate is the most frequently used phosphate fertilizer, raw material is land phosphate or phosphatic rock and 62-70% sulfuric acid, production reaction process is as follows: in the process of dissolved phosphorus dirt, emit obnoxious flavour hydrogen fluoride and silicon tetrafluoride, in the condensing works of sealing, wash with water, can be decomposed into nontoxic silicic acid and silicofluoric acid.The waste water water white transparency of discharging from this process, is slightly acidic.Therefore the production of calcium superphosphate is often combined with the production of Sodium Silicofluoride, with water wash waste gas and reclaim Sodium Silicofluoride, just becomes the method for wastewater treatment.Sodium Silicofluoride after filtration, washing, mummification can become a kind of valuable byproduct.Waste water is further processed as need, can pass through Wingdale depth filtration, deep bed filtration, or neutralizes with limestone powder or lime slurry, and the waste water after neutralization is discharged through precipitation, main sodium chloride-containing and calcium in throw out.It is large that this method is processed waste water investment, as the sewage disposal device investment of middle-size and small-size phosphate fertilizer plant reaches more than 100,000 yuan, and a year operational cost also needs more than 90,000 yuan, floor space is large, troublesome poeration, it is larger that treatment effect is affected by weather, temperature and production technique, complex process, in lime and after the waste residue problem of stacking be also difficult to solve, easily cause secondary pollution.What is more, and many phosphate fertilizer plants directly enter river course or municipal sewage system by undressed waste water by open drain or secret tunnel, and environmental and human health impacts is caused and had a strong impact on, and also can cause the secondary pollutions such as body eutrophication.
In order to solve the deficiency of current technology, I have invented a kind of phosphate fertilizer plant's waste water that utilizes and have cultivated half-naked boat-shaped algae method, can low-cost processes phosphate fertilizer plant waste water, can obtain again the biological product-half-naked boat-shaped algae of important food value, turn waste into wealth, turn harm into good, comprehensive utilization benefit is good, can significantly reduce sewage treatment equipment investment and running expense, achieve many things at one stroke, be the optimal selection of phosphate fertilizer plant's wastewater treatment.
Half-naked boat-shaped algae (Navicula seminulum) belongs to Bacillariophyta, plumage line Diatomacae, boat-shaped Cutleriales, Naviculaceae, Navicula.Navicula is the genus of most species in diatom, nearly thousand kinds.Half-naked boat-shaped algae is the main open-mouthed baits of the economic marine animal young such as abalone, stichopus japonicus.Because just Stichopus japonicus individuality abnormal and that adhere to is fine, a little less than environmental compatibility, growth is slow, and the high-quality bait that amount foot of fine quality is now provided particularly half-naked boat-shaped algae is to improve sea cucumber surviving rate, determine that sea cucumber supports the key link of industry success.Half-naked boat-shaped algae has multiple bioactive sulfated polysaccharides to nutrient solution secretion in process of growth, this polysaccharide not only can be used as wide spectrum immunopotentiating agent, improve immunological competence, in addition the effect such as anti-infective, anticoagulation, hypoglycemic, prevention and treatment tumour and AIDS.Biochemical analysis shows, half-naked boat-shaped algae also contains multiple high unsaturated fatty acid, wherein the composition such as gamma-linolenic acid, timnodonic acid, docosahexenoic acid has remarkable prophylactic effect to diseases such as cardiovascular disorder, hyperlipidemia, tumour and inflammation, be one of marine organisms that have potential pharmacy value, there is wide DEVELOPMENT PROSPECT.The long 11-30 μ of half-naked boat-shaped algae m, wide 6-20 μ m, cell boat-shaped is to oval, and wide two ends, middle part are sharp.On shell face center line, there is shell seam, can be freely movable.Except the longitudinal axis is symmetrical, transverse axis and girdle axle are also symmetrical.The longitudinal axis is long, and transverse axis is short, and girdle axle is generally shorter than transverse axis, and shell face decorative pattern is symmetrical, is strain line.There is central tubercle in shell central authorities, and Ge You one end, two ends tubercle is projecting inward to wall, have the siliceous cell wall effect of reinforcement, do not have boat's beam prominent.Chromatoplast 2-4, there is auxospore.Optimal light intensity 3000-8000Lux, optimum temperuture 21-28 ℃, optimal salinity 25-34, optimal pH 7.2-8.5.
Half-naked boat-shaped algae culture medium formula is traditionally: KNO
3600 milligrams, Na
2hPO
412H
2o60 milligram, FeCl
312 milligrams, Na
2s iO
39H
2o700 milligram, 4.3 milligrams of sodium ethylene diamine tetracetates, 3.9 milligrams of ironic citrates, vitamins B
120.5 microgram, vitamins B
110 micrograms, the pure seawater of 1000 milliliters of sterilizations, adopts plastic tank or Plastic film Bag to cultivate in the past, and growth is slow, easily pollute, cost is high, and benefit is low, operation effort, workload is large, and I adopt improved rectangle raceway pond cultivation and in substratum, have added phosphate fertilizer plant's waste water now, water glass, sodium platinate, chicken manure leach liquor, Xylo-Mucine, propylene glycol alginate, the different anti-hematic acid sodium of D-, sodium thioglycolate, L-arginine, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, soil extract, ooze leach liquor, Trypsin, Tutofusin tris, urea, oxyacetic acid, titanium solution, yeast soaks powder, Sodium desoxycholate, horsehit leach liquor, sodium ethylene diamine tetracetate, the nutritive ingredients such as ironic citrate, fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, equilibrium, easy to operate, cost is low, high efficiency, the half-naked boat-shaped algae speed of growth and output significantly improve, and culture cycle shortens, and output improves 360%.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides a kind of phosphate fertilizer plant's waste water that utilizes and cultivate half-naked boat-shaped algae culture medium, fill a prescription as follows:
85 milliliters of phosphate fertilizer plant's waste water, water glass 20 micrograms, sodium platinate 7 micrograms, 1 milliliter of chicken manure leach liquor, 3 milligrams of Xylo-Mucines, 2 milligrams of propylene glycol alginates, 5 milligrams, the different anti-hematic acid sodium of D-, 6 milligrams of sodium thioglycolates, 3 milligrams of L-arginines, 12 milligrams of medical stone powders, 920 plant growth substance 3 international unit, 1.5 milliliters of vitamin(e) S-3 solution, 1.5 milliliters of M-F trace element solutions, 1.5 milliliters of soil extracts, 1.8 milliliters of ooze leach liquors, 15 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 6 milligrams, urea, 2 milliliters of oxyacetic acids, 2 milliliters of titanium solutions, yeast soaks 8 milligrams, powder, 3 milligrams of Sodium desoxycholates, 2 milliliters of horsehit leach liquors, 1 milligram of sodium ethylene diamine tetracetate, 3 milligrams of ironic citrates, 1000 milliliters, pure seawater after clorox sterilization, utilize phosphate fertilizer plant's waste water to cultivate a half-naked boat-shaped algae method, concrete steps are as follows:
Culture device is a rectangle raceway pond, and long 50 meters, wide 25 meters, east-west, to receive more solar irradiations, raceway pond width is 1.5 meters, and raceway pond is mixed brick structure, dark 65 centimetres of pond tank, and during working order, the algae liquid depth of water is 50 centimetres.In the middle of raceway pond, peace one waterwheel, is driven by electric motor, and the waterwheel of rotation drives nutrient solution to flow in rectangle raceway pond, waterwheel velocity of rotation should not be too fast, as long as keep algae liquid to flow, frustule does not sink, and can form too soon compared with large shearing force and smash frustule.Row's glass is installed in tank top, be equivalent to lid, substratum is in a kind of semi-closed state like this, neither stop solar light irradiation algae liquid, carry out photosynthesis, the spore that can avoid again koniology, silt, leaf, paper scrap, protozoon and other microorganism etc. enters nutrient solution and pollutes.Rectangle raceway pond is washed with tap water, after use 0.2% peracetic acid disinfectant solution soaking 2 hours, the seawater flushing of sterilizing with clorox again 2 times, put into nutrient solution to 45 centimetres of the depth of waters, starting waterwheel rotates 20 minutes, stop waterwheel, select in exponential phase of growth, vigorous, the clean free of contamination algae kind inoculation of vitality, inoculum density 4 * 10
4cell/mL, after inoculation, start waterwheel and stir algae liquid, frustule is uniformly distributed, supplement nutrient solution to 50 centimetres of the depth of waters, starting waterwheel cultivates, outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-10000Lux, to the 7th day, then supplement fresh culture to 50 centimetres of the depth of waters, to make up the moisture consuming because of volatilization.To the 9th day, algae cell density reached 600 * 10
4cell/mL, stops motor, by the floor drain of cultivation pool bottom, algae liquid is emitted, and after rectangle raceway pond is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate;
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
Preferably, the described raceway pond to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, the described raceway pond to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute;
Preferably, the described raceway pond to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute;
Preferably, to the described raceway pond of selecting, minute two sterilized phases after washing, are first used high pressure nitrogen to spray sterilization, sterilization 2-3 minute; Use high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute; After sterilization, carry out again algae culture.Useful result:
In order to overcome Gourmet Powder Factory's waste water traditional treatment method shortcoming, I have invented a kind of phosphate fertilizer plant's waste water that utilizes and have cultivated half-naked boat-shaped algae method, not only can low-cost processes phosphate fertilizer plant waste water, utilization of waste material, turn waste into wealth, and can obtain the half-naked boat-shaped algae of important food value, achieve many things at one stroke, really be the ingenious utmost point, simply fantastic.Half-naked boat-shaped algae is cultivated traditional culture medium prescription and is: KNO
3600 milligrams, Na
2hPO
412H
2o60 milligram, FeCl
312 milligrams, Na
2s iO
39H
2o700 milligram, 4.3 milligrams of sodium ethylene diamine tetracetates, 3.9 milligrams of ironic citrates, vitamins B
120.5 microgram, vitamins B
110 micrograms, the pure seawater of 1000 milliliters of sterilizations, and adopt plastic tank or Plastic film Bag to cultivate, grow slow, easily pollute, cost is high, and benefit is low, operation effort, workload is large, and I adopt improved rectangle raceway pond cultivation and in substratum, have added phosphate fertilizer plant's waste water now, water glass, sodium platinate, chicken manure leach liquor, Xylo-Mucine, propylene glycol alginate, the different anti-hematic acid sodium of D-, sodium thioglycolate, L-arginine, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, soil extract, ooze leach liquor, Trypsin, Tutofusin tris, urea, oxyacetic acid, titanium solution, yeast soaks powder, Sodium desoxycholate, horsehit leach liquor, sodium ethylene diamine tetracetate, the nutritive ingredients such as ironic citrate, fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, equilibrium, easy to operate, cost is low, high efficiency, the half-naked boat-shaped algae speed of growth and output significantly improve, and culture cycle shortens, and output improves 360%.
Description of drawings 1 is half-naked boat-shaped algae morphological structure figure;
Wherein Reference numeral is: 1 end tubercle, 2 central tubercles, 3 shell seams, 4 strain lines;
Fig. 2 is for utilizing phosphate fertilizer plant's waste water to cultivate half-naked boat-shaped algae method figure;
Wherein Reference numeral is: 5 electric motor, the impeller of 6 waterwheels, 7 glass, 8 brick walls, 9 racetrack culturing pools.
Embodiment
1) phosphate fertilizer plant's pretreatment method for wastewater: phosphate fertilizer plant's waste water needs to carry out pre-treatment before supporting algae, method is: first use 60 object silk cover filtering waste water, remove floating matter, glass bits, hair, silt, leaf, the larger solid substance of plastics bag equal-volume, after sewage is introduced in long 30 meters, wide 20 meters, the cement aeration tank of dark 0.7 meter, it is east-west that pond is, to accept more solar radiation, the depth of water remains on 60 centimetres, (unslaked lime preferably adds the amount of 5 liters of pure water to dissolve in plastic tank by 1 kilogram to unslaked lime in advance by every cubic metre of sewage, to add the amount of 50 grams of unslaked limes evenly to splash, dilution, stir, full pool spilling head again), every 60 minutes, (alum preferably dissolves in plastic tank by 100 grams of amounts that add 4 liters of pure water in advance by every cubic metre of sewage, to add the amount full pool spilling head alum of 80 grams again, dilute and stir, full pool spilling head again), then with rake, stir or air agitation 60 minutes, lime and alum are fully dissolved, mix and with sewage in organism fully there is chemical reaction, then natural sedimentation, fermentation, expose 72 hours, skim the floating matters such as water surface foam, at the bottom of not stirring pond, get sewage at the middle and upper levels, pack in lighttight black plastic bucket, sealing, 4 ℃ of refrigerators or freezer, preserve, standby,
2) titanium solution is joined method: 736.6 milligrams of titanous oxalates, be dissolved in 100 ml distilled waters, add 5 grams of ammonium hydroxide, make it alkalize, precipitate, by centrifuging, will precipitate and concentrate, with the sulfuric acid dissolution of 20 milliliters of 0.05mol/L and get final product.
3) M-F trace element solution is joined method: 0.2 gram of boric acid, and 0.2 gram of Manganous chloride tetrahydrate, 0.2 milligram of molybdic oxide, 0.02 gram, copper sulfate, 0.02 gram, zinc sulfate, 0.02 gram of cobalt chloride, is dissolved in 1000 ml distilled waters, finally splashes into a vitriol oil, shakes up and get final product.
4) vitamin(e) S-3 solution is joined method: 50 milligrams of thiamine salt hydrochlorates, and 10 milligrams of Nicotinicum Acidums, 10 milligrams of calcium pantothenate, 1.0 grams of para-amino benzoic acid, 0.1 gram of vitamin H, 0.5 gram of inositol, folic acid 0.2 microgram, between chest, pyridine is 0.3 gram, 1000 milliliters of distilled water;
5) clorox sterilization seawater method: get seawater, absorbent cotton filters, and by adding the amount of 75 grams, chlorinated lime to add chlorinated lime in 1 cubic metre of seawater, makes available chlorine content in seawater reach 20mg/L, inflates after 12 hours, uses in Sulfothiorine and chlorine residue.Sulfothiorine amount is determined by the number of chlorine residue, until splash into the nondiscoloration of starch potassium iodide liquid;
6) peracetic acid disinfectant liquid and preparation method thereof and application notice: the former liquid hold-up 35% of commercially available Peracetic Acid (by weight), colourless liquid, has intense stimulus smell.During use, apply clean tap water and be diluted to 0.2% solution, soak raceway pond inwall 2 hours.These product have intense stimulus effect to eyes, skin, mucous membrane and the upper respiratory tract, headache, nauseating and inflammable, and tool explosivity, severe corrosive, strong and stimulating, can cause human body and burn, and is heated to 100 ℃ of i.e. fierce decomposition, meets fiery or be heated, be subject to shake all can detonate.Contact with reductive agent, promotor, organism, combustiblematerials etc. vigorous reaction can occur; Not directly catch tactile, during operation, should be with rubber gloves, mouth mask and glasses, metal is corrosive, be not useable for the sterilization of metallic weapon; The long-term placement of stoste can be decomposed, and notes the quality guaranteed period, should store in cool dark place in plastic tank and preserve.
7) the half-naked boat-shaped algae of inoculation use is purified (this area ordinary method, as long as can realize purification, Kai Shi extraction method for example) in advance, and algae kind is in eugonic exponential phase of growth when guaranteeing to inoculate;
8) various nutritive salt will add by the order providing in formula, to avoid chemical reaction;
9) various inorganics nutritive salt and VITAMIN need be made in advance mother liquor and be placed in 4 ℃ of refrigerator cold-storages and save backup, and storage period is not over 45 days;
10) chicken manure leach liquor preparation method: dried poultrymanure, green grass, soya-bean cake, rice bran, grain husk shell (shells of five cereals) and vegetable mould were by 60: 10: 12: the part by weight of 15: 2: 1 mixes, stir, pack airtight fermentation in cylinder into, after 30 days, pour out, at clean water mire, stand lamellar, dry 48 hours, after get 2 kilograms, be placed in plastic bucket, add 10 kilograms, tap water, stir, with 80 order silk cover filterings, clear liquid is boiled 5 minutes, cooling, standing 24 hours, get supernatant liquor, pack reagent bottle into, labelled, be placed in 4 ℃ of refrigerators, standby,
11) soil Extract preparation method: loose to getting in the woods, be rich in 1 kilogram of organic upper layer of soil, add 5 liters, tap water, boil 20 minutes, mud is poured in beaker, standing 24 hours, rear absorption supernatant liquor, pour in Erlenmeyer flask, add cotton plug, boil 15 minutes, cooling, staticly settle 12 hours, get supernatant liquor and obtain soil Extract, pack grinding port plug reagent bottle into, sealing, labelled, be placed in 4 ℃ of refrigerators standby;
12) medical stone powder is the powder product of medical stone, by medical stone raw ore, through screening, is pulverized, and is processed into floury, and granular size can be passed through 200 mesh sieve thin,tough silk;
13) 920 plant growth substances are white crystalline powder, are difficult for being dissolved in water, can first with alcohol, fully dissolve (general every gram dissolves more than 1 hour with 50 ml ethanols or 60 degree white wine), then be watered dilution by desired concn;
14) horsehit leach liquor preparation method: get 50 kilograms of fresh horsehits, add 3 kilograms, wheat bran, 1 kilogram of Semen Maydis powder, 4 liters, tap water, fully stir, pile coniform, spontaneous fermentation 15 days, rear stand lamellar, under sunlight, be exposed to the sun 3 days, after get 3 kilograms and put into bucket, add 10 liters of distilled water, fully stir evenly, standing, 100 order silk cover filterings after 48 hours, get filtrate heating, boil 45 minutes, kill bacterium wherein, mould, virus etc., cooling, precipitate 48 hours, get supernatant liquor, obtain horsehit leach liquor, pack grinding port plug reagent bottle into, sealing, be placed in 4 ℃ of refrigerators standby,
15) ooze extracts liquid and preparation method thereof: get the more fertile black ooze of what not again, with 1 part of mud, add 2 parts of fresh water, stir, standing 1-2 minute, get upper strata mud in aluminum pot, the amount that adds 1 gram of NaOH by every 1000 milliliters of mud adds NaOH, boils 15 minutes.While boiling, need constantly stir, boil that within latter standing 24 hours, to get supernatant liquor standby;
16) pharmaceutical chemicals purity used all needs chemical pure rank;
17) in order to overcome the deficiency of current technology, the invention provides a kind of substratum that utilizes phosphate fertilizer plant's waste water to cultivate half-naked boat-shaped algae, fill a prescription as follows:
18) phosphate fertilizer plant's waste water is 85 milliliters, water glass 20 micrograms, sodium platinate 7 micrograms, 1 milliliter of chicken manure leach liquor, 3 milligrams of Xylo-Mucines, 2 milligrams of propylene glycol alginates, 5 milligrams, the different anti-hematic acid sodium of D-, 6 milligrams of sodium thioglycolates, 3 milligrams of L-arginines, 12 milligrams of medical stone powders, 920 plant growth substance 3 international unit, 1.5 milliliters of vitamin(e) S-3 solution, 1.5 milliliters of M-F trace element solutions, 1.5 milliliters of soil extracts, 1.8 milliliters of ooze leach liquors, 15 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 6 milligrams, urea, 2 milliliters of oxyacetic acids, 2 milliliters of titanium solutions, yeast soaks 8 milligrams, powder, 3 milligrams of Sodium desoxycholates, 2 milliliters of horsehit leach liquors, 1 milligram of sodium ethylene diamine tetracetate, 3 milligrams of ironic citrates, 1000 milliliters, pure seawater after clorox sterilization,
19) utilize phosphate fertilizer plant's waste water to cultivate a half-naked boat-shaped algae method, concrete steps are as follows:
Culture device is a rectangle raceway pond, and long 50 meters, wide 25 meters, east-west, to receive more solar irradiations, raceway pond width is 1.5 meters, and raceway pond is mixed brick structure, dark 65 centimetres of pond tank, and during working order, the algae liquid depth of water is 50 centimetres.In the middle of raceway pond, peace one waterwheel, is driven by electric motor, and the waterwheel of rotation drives nutrient solution to flow in rectangle raceway pond, waterwheel velocity of rotation should not be too fast, as long as keep algae liquid to flow, frustule does not sink, and can form too soon compared with large shearing force and smash frustule.Row's glass is installed in tank top, be equivalent to lid, nutrient solution is in a kind of semi-closed state like this, neither stop solar light irradiation algae liquid, carry out photosynthesis, the spore that can avoid again koniology, silt, leaf, paper scrap, protozoon and other microorganism etc. enters nutrient solution and pollutes.Rectangle raceway pond is washed with tap water, after use 0.2% peracetic acid disinfectant solution soaking 2 hours, the seawater flushing of sterilizing with clorox again 2 times, put into nutrient solution to 45 centimetres of the depth of waters, starting waterwheel rotates 20 minutes, stop waterwheel, select in exponential phase of growth, vigorous, the clean free of contamination algae kind inoculation of vitality, inoculum density 4 * 10
4cell/mL, after inoculation, start waterwheel and stir algae liquid, frustule is uniformly distributed, supplement nutrient solution to 50 centimetres of the depth of waters, starting waterwheel cultivates, outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-10000Lux, to the 7th day, then supplement fresh medium to 50 centimetres of the depth of waters, to make up the moisture consuming because of volatilization.To the 9th day, algae cell density reached 600 * 10
4cell/mL, stops motor, by the floor drain of cultivation pool bottom, algae liquid is emitted, and after rectangle raceway pond is washed, after rectangle raceway pond is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate;
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (6)
1. utilize phosphate fertilizer plant's waste water to cultivate a half-naked boat-shaped algae culture medium, it is characterized in that formula is as follows:
85 milliliters of phosphate fertilizer plant's waste water, water glass 20 micrograms, sodium platinate 7 micrograms, 1 milliliter of chicken manure leach liquor, 3 milligrams of Xylo-Mucines, 2 milligrams of propylene glycol alginates, 5 milligrams, the different anti-hematic acid sodium of D-, 6 milligrams of sodium thioglycolates, 3 milligrams of L-arginines, 12 milligrams of medical stone powders, 920 plant growth substance 3 international unit, 1.5 milliliters of vitamin(e) S-3 solution, 1.5 milliliters of M-F trace element solutions, 1.5 milliliters of soil extracts, 1.8 milliliters of ooze leach liquors, 15 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 6 milligrams, urea, 2 milliliters of oxyacetic acids, 2 milliliters of titanium solutions, yeast soaks 8 milligrams, powder, 3 milligrams of Sodium desoxycholates, 2 milliliters of horsehit leach liquors, 1 milligram of sodium ethylene diamine tetracetate, 3 milligrams of ironic citrates, 1000 milliliters, pure seawater after clorox sterilization.
2. utilize phosphate fertilizer plant's waste water to cultivate a half-naked boat-shaped algae method, it is characterized in that concrete steps are as follows:
Culture device is a rectangle raceway pond, long 50 meters, wide 25 meters, east-west, to receive more solar irradiations, raceway pond width is 1.5 meters, raceway pond is mixed brick structure, dark 65 centimetres of pond tank, during working order, the algae liquid depth of water is 50 centimetres, peace one waterwheel in the middle of raceway pond, by electric motor, driven, the waterwheel rotating drives nutrient solution to flow in rectangle raceway pond, waterwheel velocity of rotation should not be too fast, as long as keep algae liquid to flow, frustule does not sink, can form too soon compared with large shearing force and smash frustule, row's glass is installed in tank top, be equivalent to lid, nutrient solution is in a kind of semi-closed state like this, neither stop solar light irradiation algae liquid, carry out photosynthesis, can avoid koniology again, silt, leaf, paper scrap, the spore of protozoon and microorganism enters nutrient solution and pollutes, rectangle raceway pond is washed with tap water, after use 0.2% peracetic acid disinfectant solution soaking 2 hours, the seawater flushing of sterilizing with clorox again 2 times, put into substratum claimed in claim 1 to 45 centimetres of the depth of waters, starting waterwheel rotates 20 minutes, stop waterwheel, selection is in exponential phase of growth, vitality is vigorous, clean free of contamination algae kind inoculation, inoculum density 4 * 10
4cell/mL, starts waterwheel and stirs algae liquid after inoculation, frustule is uniformly distributed, supplement nutrient solution to 50 centimetres of the depth of waters, start waterwheel and cultivate, outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-10000Lux, to the 7th day, supplement again fresh substratum claimed in claim 1 to 50 centimetres of the depth of waters, to make up the moisture consuming because of volatilization, to the 9th day, algae cell density reached 600 * 10
4cell/mL, stops motor, by the floor drain of cultivation pool bottom, algae liquid is emitted, and after rectangle raceway pond is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate,
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
3. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
4. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute.
5. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute.
6. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, to the described reactor of selecting, minute two sterilized phases after washing, first use high pressure nitrogen to spray sterilization, sterilization 2-3 minute, is used high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute, carries out algae culture again after sterilization.
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