CN103960118B - A kind of substratum and cultural method utilizing wastewater of starch factory cultivation Platymonas helgolandica var - Google Patents
A kind of substratum and cultural method utilizing wastewater of starch factory cultivation Platymonas helgolandica var Download PDFInfo
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- CN103960118B CN103960118B CN201410222840.2A CN201410222840A CN103960118B CN 103960118 B CN103960118 B CN 103960118B CN 201410222840 A CN201410222840 A CN 201410222840A CN 103960118 B CN103960118 B CN 103960118B
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses one utilizes wastewater of starch factory to cultivate Platymonas helgolandica var method.This technology not only can low-cost processes starch wastewater, and turning waste into wealth, also can obtain the Platymonas helgolandica var of important food value, kill two birds with one stone, is ingenious pole really.Platymonas helgolandica var is cultivated and is used f/2 substratum traditionally and use open cement track pond, growth is slow, yield poorly, invest large, easily pollute, benefit is low.Now I adopt volume be 3 cubic metres high density polyethylene(HDPE) water tower cultivate, cost is low, not easily pollute, scrub conveniently, can reuse, easy handling, and the present invention adds the nutritive ingredient such as donkey excrement leach liquor, SODIUM PHOSPHATE, MONOBASIC, propylene glycol alginate, Sorbitol Powder, ooze extraction liquid, soil Extract, ethylenediamine tetraacetic acid (EDTA), vitamin complex, people's urine in minimum medium, fertilizer and inorganic fertilizer used in combination, nutrition is more comprehensive, balanced, Platymonas helgolandica var output increased 300%.
Description
Technical field
The invention belongs to aquaculture field, particularly relate to a kind of substratum and the cultural method that utilize wastewater of starch factory cultivation Platymonas helgolandica var.
Background technology
Starch is a kind of essential industry raw material, is widely used in food, chemical industry, weaving, pharmaceutical industries.In starch production process, wastewater discharge is large, often produces 1t starch and produces 10-20m
3waste water, major ingredient is starch, protein and sugar class, and COD value of waste water, at 8000-30000mg/L, BOD value 5000-20000mg/L, SS value 3000-5000mg/L, pH4-6, belongs to high content acid wastewater.In addition, some starch wastewater is also containing a certain amount of SO
3 2-, SO
4 2-.Current China has size starch factory more than 300 family, often produces waste water 1,600 ten thousand m per year
3containing solvability starch, a small amount of protein, organic acid, dust, mineral substance and grease, easily corrupt, turn black smelly, enter rivers and consume dissolved oxygen in water, promote algae and waterplant breeding, cause rivers anoxic, anaerobism is corrupt, distribute stench, severe patient fish, shrimp, shellfish death by suffocation, also form grave danger to HUMAN HEALTH, therefore starch wastewater is administered and utilization is subject to environmental science worker attention.Starch wastewater treatment technique comparatively conventional both at home and abroad at present has following several:
1. anaerobic biological process
Anaerobic biotreatment method is under anaerobic, by anaerobic bacteria decomposing organic matter.In the process, participate in the organic 50-90% of biological degradation and be converted into biogas, the residuum after fermentation can be used as fertilizer and feed.Anaerobic process mainly contains up-flow anaerobic sludge blanket, deflector anaerobic Sludge Bed, anaerobic active sludge method, EGSB (expanded granular sludge bed) etc.Experiment shows, anaerobic process not only pollutant removal is obvious, and process stabilizing, energy consumption is low, and sludge yield is few, also can produce methane equal energy source gas, therefore be widely used.But anaerobic treatment method is subject to the factor impacts such as waste water water temperature, pH, toxic substance and the organic concentration of water outlet is high, the culturing sludge cycle is long.
2. aerobe method
Be under the condition of oxygen abundance, the vital movement making good use of oxygen animalcule is oxidized dirty Organic substance in water, and product is carbonic acid gas, water and energy.Activated sludge process, biomembrance process, deep well aeration method are representational aerobic biological process for treating.Recent two decades has carried out great many of experiments to Aerobic biological process method both at home and abroad, in high concentrated organic wastewater process, achieves certain achievement, COD clearance 95%, and reclaim protein 2.07-2.39g/L waste water, protein nontoxicity, do that animal feed efficiency is good and technique is simple, easy to operate, capital construction and running cost low, be convenient to management, shortcoming needs oxygenation, power consumption is large, and noenergy reclaims, and microorganism desired nutritional is many.
3. biological pond method
Be that one utilizes water body natural detergent power process method for waste water, this technology develops rapidly after the 1950's, applies at most in sanitary sewage and Industrial Wastewater Treatment.High for starch wastewater concentration, be rich in nutrition characteristic, adopt anaerobic pond, facultative lagoon, aerobic ponds combine biological pond technology.Yang Fengjiang research shows, adopt oxidation pond-Herba Eichhorniae pond-fine duckweed pond process, waste water is after barrier precipitation, import oxidation pond, through spontaneous fermentation in 1-2 days, then import Herba Eichhorniae pond, through Herba Eichhorniae purification in 7 days, reduce Pollutant levels and achieve waste water resource and utilize, but oxidation pond process is taken up an area many, is subject to weather effect.
4. flocculent precipitation
By adding flocculation agent, reducing colloidal solution stability, making it coagulative precipitation, isolation of purified, small suspended substance and colloidal impurity in main process water.Flocculation agent used is microorganism and polyose flocculation agent mainly, also has Polyferric Sulfate class flocculation agent.Waste water COD cr clearance 96.2%, turbidity removal rate reaches 96.4%.This method initial cost is few, technique is simple, easy to operate, energy consumption is low, to temperature Change strong adaptability, but coagulating agent price, the low and easily secondary pollution of small organic molecule clearance.
5. Photosynthetic Bacteria
The seventies in last century, the photosynthetic bacterium process organic waste waters that take the lead in utilizing such as the positive Thailand of holt of Japan, effect is better.Photosynthetic bacterium is Rhodopseudomonas mainly, can under anaerobic carry out not oxygen consumption photosynthesis, utilize organism as Photosynthetic Carbon and hydrogen donor, can bear high-enriched organics, not need to dilute direct process.Under suitable condition, CODcr clearance reaches 95.7%.This method organic removal rate is high, reduced investment, occupation of land less and bacterial classification mud is nontoxic to people and animals, have a bright future.But Photosynthetic Bacteria is responsive to temperature variation equally, need heat and attemperator, need stronger incandescent light photograph in the evening, and working cost is high and management is inconvenient.
In order to overcome starch wastewater traditional treatment method shortcoming, my invention's one utilizes wastewater of starch factory to cultivate Platymonas helgolandica var technology, not only can low-cost processes starch wastewater, utilization of waste material, turn waste into wealth, and the Micro Algae of important food value can be obtained--Platymonas helgolandica var, kills two birds with one stone, really be ingenious pole, simply fantastic.
Platymonas helgolandica var Platymonassp. belongs to Chlorophyta, Chlorophyceae, volvocales, chlamydomonas section, flat algae genus.Row monogony, cell longitudinal division becomes 2, is 4 under a few cases, forms statospore when environment is bad.Cell is extensively avette, and front end is broader, caves in top; Flagellum 4 is born by hollow; Have a large-scale cup-shaped green pigment body in cell, there is an internal rising cup shape pyrenoids nearly rear end, has one or more red eyespots near pyrenoids, has a nucleus, cell outsourcing layer cellulosic cell walls before omiting in the middle of cell; Travelling in water by flagellum, rapidly vivaciously; The long 16-30 micron of body, wide 12-15 micron, thick 7-10 micron, symmetrical, have dividing of back of the body abdomen, back is swelled, and belly is slightly recessed; Afterbody is slightly narrow or slightly sharp, and eyespot 2-3, minority has 4; Equal energy growth and breeding in salinity 8-80 water, optimal salinity 30-40; Thermal adaptation scope is also comparatively wide, 7-30 DEG C, the suitableeest 20-28 DEG C.Optimal light intensity 5000-10000Lux; Optimal pH 7.5-8.5.This algae strong adaptability, growth and breeding is rapid, very easily cultivate, the extra large precious animal young high-quality bait such as Chinese prawn, shrimp, chlamys farreri, bay scallop, mussel, abalone, tongue sole, swimming crab, blood clam, mud blood clam, oyster, sea cucumber, be rich in the necessary multivitamin of the animal young and lipid acid, growing larval growth has effect of the utmost importance.Also fabulous nutritional fortification effect is had to grow necessary animal bait wheel animalcule, naked abdomen egg, fairy shrimp etc. of Hai Zhen.Platymonas helgolandica var is cultivated and is used f/2 substratum traditionally and use open cement track pond, growth is slow, yield poorly, invest large, easily pollute, benefit is low.I adopts volume to be that the water tower with fabulous light transmission that the high density polyethylene(HDPE) of 3 cubic metres is made is cultivated now, cost is low, not easily pollute, scrub conveniently, can reuse, easy handling, and I with the addition of donkey excrement leach liquor in starch wastewater, rabbit excrement leach liquor, sodium acetate, ammonium sulfate, magnesium sulfate, SODIUM PHOSPHATE, MONOBASIC, propylene glycol alginate, Sugaron, Sorbitol Powder, Tripolyglycerol monostearates, ooze extracts liquid, soil Extract, ethylenediamine tetraacetic acid (EDTA), vitamin complex, the nutritive ingredients such as people's urine, fertilizer and inorganic fertilizer used in combination, nutrition is more comprehensive, balanced, the Platymonas helgolandica var speed of growth increases, culture cycle shortens, output increased 300%.
Beneficial outcomes: in order to overcome wastewater of starch factory traditional treatment method shortcoming, a kind of method utilizing wastewater of starch factory to cultivate Platymonas helgolandica var of my invention, not only utilization of waste material, turn waste into wealth, and the flat algae of important food value can be obtained, killing two birds with one stone, is ingenious pole really, simply fantastic.Platymonas helgolandica var is cultivated and is used f/2 substratum traditionally and use open cement pit to cultivate, throughput rate is low, output is not high, investment is large, easy pollution, benefit is low, I adopts volume to be that the water tower with fabulous light transmission that the high density polyethylene(HDPE) of 3 cubic metres is made is cultivated now, cost is low, not easily pollute, scrub conveniently, can reuse, easy handling, and I adds donkey excrement leach liquor in wastewater of starch factory, rabbit excrement leach liquor, sodium acetate, ammonium sulfate, magnesium sulfate, SODIUM PHOSPHATE, MONOBASIC, propylene glycol alginate, Sugaron, Sorbitol Powder, Tripolyglycerol monostearates, ooze extracts liquid, soil Extract, ethylenediamine tetraacetic acid (EDTA), vitamin complex, the nutritive ingredients such as people's urine, fertilizer and inorganic fertilizer used in combination, nutrition is more comprehensive, balanced, the Platymonas helgolandica var speed of growth and output significantly improve, culture cycle shortens, output increased 300%.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides a kind of substratum utilizing wastewater of starch factory to cultivate Platymonas helgolandica var, filling a prescription as follows:
Wastewater of starch factory 75 milliliters, donkey excrement leach liquor 2.0 milliliters, rabbit excrement leach liquor 1.5 milliliters, sodium acetate 12 milligrams, 18 milligrams, ammonium sulfate, 24 milligrams, magnesium sulfate, 30 milligrams, agar, sucrose 25 milligrams, SODIUM PHOSPHATE, MONOBASIC 15 milligrams, propylene glycol alginate 10 milligrams, Sugaron 8 milligrams, Sorbitol Powder 1.0 milliliters, Tripolyglycerol monostearates 5 milligrams, ooze extracts liquid 1.5 milliliters, soil Extract 2.0 milliliters, ethylenediamine tetraacetic acid (EDTA) 6 milligrams, vitamins B
112 micrograms, vitamins B
121.5 micrograms, people urinates 2 milliliters, 1000 milliliters, the pure seawater after hypochlorite disinfectant.
A kind of Platymonas helgolandica var cultural method, concrete steps are as follows:
Culture vessel is right cylinder polyethylene water tower, volume 3.0 cubic metres, and high-density polyethylene material is made (commercially available), colourless or white, there is good light transmission, carry out photosynthesis in order to algae, water tower is integral type structure, and wall thickness 6 millimeters, has very large intensity and soundness.Column head has a circular lid, above has and revolves silk, rotatable and seal.The base that an angle bar is welded into bottom water tower, high 50 centimetres.Column head lid diameter 40 centimetres, lid stamps 2 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, internal diameter 10 millimeters.Reactor is washed, rear distilled water rinse 3 times, load substratum according to claim 1 2000 liters, inflation mixing 5 minutes, select vigorous, the free of contamination Platymonas helgolandica var inoculation of vitality, inoculum density 2 × 10
4cell/mL; Lid inserts two clean Glass tubings, and one longer, 65 centimetres, 12 centimetres, lid is exposed on top, lower end leads to charge tube and is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone forms very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 30 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing, internal diameter 10 millimeters; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, and be filled with mixed gas, mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, can not float with frustule; Outside scenery, solar light irradiation, optimum temps 25 ± 2 DEG C, optimum illumination intensity 3000-10000Lux; First 5 days that cultivate, build a simple and easy canopy above water tower, upper placement curtain shut out the light, and makes intensity of illumination maintain 1500-2800Lux, avoided the light injury that sunburst causes cell; Within 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and the synthesis of high unsaturated fatty acid; Be cultured to the 7th day, stop inflation, take away lid, supplement fresh culture 100 liters, cover lid, inflation, continue to cultivate, to the 9th day, Platymonas helgolandica var density was increased to 150 × 10
4cell/mL, stops cultivating, releases algae liquid, after being washed by culture vessel, add 0.2% peracetic acid disinfectant solution soaking and sterilize 2 hours, then use distilled water rinse one time from the water tap of bottom, after enter next round and cultivate;
Preferably, after being washed by culture vessel, do not adopt the disinfection way of " adding 0.2% peracetic acid disinfectant solution soaking to sterilize 2 hours ", and adopt " add disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round cultivate.Described Traditional Chinese medicine disinfectant is made up of the material of following weight ratio: the Chinese medicinal materials adding following weight part in every 100 part of 85% ethanol: Root of Indigowoad 30 ~ 50 parts, radix bupleuri 15 ~ 25 parts, peppermint 15 ~ 25 parts, cape jasmine 5 ~ 15 parts, tarragon 5 ~ 15 parts, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine fruit chopping, dry at 60-70 DEG C, 70 orders are crushed to respectively more for subsequent use with Chinese medicine material crushing machine, take respectively with table balance and dry and the Root of Indigowoad 120 grams pulverized, radix bupleuri 60 grams, mentha leave 60 grams, tarragon 30 grams, mountain Cape jasmine fruit 30 grams, after being placed in the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black agents bottle, labelled, be placed in 4 DEG C of refrigerators for subsequent use.”
Preferably, to the reactor described in selecting, carry out disinfection after washing, use ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, to the reactor described in selecting, carry out disinfection after washing, use high pressure nitrogen to spray sterilization, sterilization 5-10 minute;
Preferably, to the reactor described in selecting, carry out disinfection after washing, use high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute;
Preferably, to the reactor described in selecting, point two sterilized phases after washing, first use high pressure nitrogen to spray sterilization, sterilization 2-3 minute; High-pressure carbon dioxide is used to spray sterilization, sterilization 2-3 minute; Algae culture is carried out again after sterilization.
Description of drawings 1 utilizes wastewater of starch factory to cultivate Platymonas helgolandica var method figure for one.
Wherein Reference numeral is: 1 gas-filled valve 2 algae liquid 3 base 4 culture vessel 5 escape pipe.
Embodiment
1) starch wastewater pretreatment process: starch wastewater needs to carry out pre-treatment before being used for supporting algae, method is: first use 60 object silk cover filtering waste water, removing floating matter, glass bits, hair, silt, leaf, the larger solid substance of plastics bag equal-volume, after sewage introduced in long 30 meters, wide 20 meters, the cement aeration tank of dark 0.7 meter, pond is east-west, to accept more solar radiation, the depth of water remains on 55 centimetres, the amount adding 50 grams of unslaked limes by every cubic metre of sewage is evenly splashed, and unslaked lime (dissolve in plastic tank by the amount that unslaked lime preferably adds 5 liters of pure water by 1 kilogram in advance, dilution, stir, full pool spilling head again), every 60 minutes, (amount that alum preferably adds 4 liters of pure water by 100 grams is in advance dissolved in plastic tank to add the amount full pool spilling head alum of 80 grams by every cubic metre of sewage again, dilute and stir, full pool spilling head again), then stir or air agitation 60 minutes with rake, lime and alum are fully dissolved, also fully there is chemical reaction with dirty Organic substance in water in mixing, then natural sedimentation, fermentation, expose 72 hours, skim the floating matters such as water surface foam, do not stir at the bottom of pond, get sewage at the middle and upper levels, boil 6 minutes, cooling, load in lighttight black plastic bucket, sealing, 4 DEG C of refrigerators or freezer, preserve, for subsequent use,
2) donkey excrement leach liquor preparation method: get 50 kilograms, fresh donkey excrement, add 1 kilogram, wheat bran, Semen Maydis powder 2 kilograms, 3 liters, tap water, abundant stirring, pile coniform, spontaneous fermentation 20 days, rear stand lamellar, be exposed to the sun under sunlight 4 days, after get 3 kilograms and put into bucket, add 15 liters of distilled water, fully stir evenly, leave standstill, 400 order silk cover filterings after 48 hours, get filtrate heating, boil 15 minutes, kill bacterium wherein, mould, virus etc., cooling, precipitate 24 hours, get supernatant liquor, obtain donkey excrement leach liquor, load grinding port plug reagent bottle, sealing, labelled, be placed in 4 DEG C of refrigerators for subsequent use,
3) hypochlorite disinfectant's seawater method: get seawater, absorbent cotton filters, and adds chlorinated lime, make available chlorine content in seawater reach 10-20mg/L by the amount adding chlorinated lime 50-100 gram in 1 cubic metre of seawater, inflate after 10-12 hour, with in Sulfothiorine and chlorine residue.Sulfothiorine amount by chlorine residue number determine, until the nondiscoloration of instillation starch potassium iodide liquid;
4) peracetic acid disinfectant preparation method and application notice: commercially available Peracetic Acid stoste content 35% (by weight), colourless liquid, has intense stimulus smell.Apply clean tap water during use and be diluted to 0.2% solution, soak water tank 2 hours.These product have intense stimulus effect to eyes, skin, mucous membrane and the upper respiratory tract, headache, to feel sick and inflammable, and tool explosivity, severe corrosive, strong and stimulating, can cause human body and burn, and are heated to 100 DEG C and namely fiercely decompose, and meet fire or being heated, all can detonating by shaking.Contact with reductive agent, promotor, organism, combustiblematerials etc. and vigorous reaction can occur; Corrodibility is strong, not directly catches tactile, should be with rubber gloves, mouth mask and glasses, be corrosive to metal during operation, be not useable for the sterilization of metallic weapon; Stoste places decomposable asymmetric choice net for a long time, notes the quality guaranteed period, should store in cool dark place in plastic tank and preserve, away from inflammable substance.
5) Platymonas helgolandica var of inoculation carries out purifying (this area ordinary method, as long as can realize purifying, such as kelvin extraction method) in advance, and during to guarantee to inoculate, algae kind is in exponential phase of growth;
6) various nutritive salt will add by the order provided in formula, to avoid chemical reaction;
7) various nutritive salt all needs to be made into mother liquor in advance and is placed in 4 DEG C of refrigerator cold-storages and saves backup, and does not exceed 45 days storage period;
8) all nutritive salt will inflate mixing 10 minutes after adding, and inoculates after nutritive salt is mixed;
9) rabbit excrement leach liquor preparation method: dry rabbit excrement, green grass, soya-bean cake, rice bran, the part by weight mixing of 60: 10: 12: 15: 2: 1 pressed by grain husk shell (shells of five cereals) and vegetable mould, stir, load airtight in cylinder fermentation, after 30 days, pour out, at clean water mire, stand lamellar, dry 48 hours, after get 2 kilograms, be placed in plastic bucket, add 10 kilograms, tap water, stir, with 200 order silk cover filterings, clear liquid is boiled 5 minutes, cooling, leave standstill 24 hours, get supernatant liquor, load reagent bottle, labelled, be placed in 4 DEG C of refrigerators, for subsequent use,
10) soil Extract preparation method: get loose, to be rich in organic matter upper layer of soil 1 kilogram in the woods, add 5 liters, tap water, boil 20 minutes, mud is poured in beaker, leave standstill 24 hours, rear Aspirate supernatant, pour in Erlenmeyer flask, add cotton plug, boil 15 minutes, cooling, staticly settles 12 hours, gets supernatant liquor and namely obtain soil Extract, load grinding port plug reagent bottle, sealing, labelled, be placed in 4 DEG C of refrigerators for subsequent use;
11) people urinates preparation method: get people and urinate 1000 milliliters, after flask boils 5 minutes, cools for subsequent use;
12), on the base that water tower basis rest is connected at an angle bar, steel pipe welding, base is high 50 centimetres, requires that base is firm, strong, stable, firm, durable, antirust;
13) pharmaceutical chemicals purity used all needs chemical pure rank;
14) in order to overcome the deficiency of current technology, the invention provides a kind of substratum utilizing wastewater of starch factory to cultivate Platymonas helgolandica var, filling a prescription as follows:
Wastewater of starch factory 75 milliliters, donkey excrement leach liquor 2.0 milliliters, rabbit excrement leach liquor 1.5 milliliters, sodium acetate 12 milligrams, 18 milligrams, ammonium sulfate, 24 milligrams, magnesium sulfate, 30 milligrams, agar, sucrose 25 milligrams, SODIUM PHOSPHATE, MONOBASIC 15 milligrams, propylene glycol alginate 10 milligrams, Sugaron 8 milligrams, Sorbitol Powder 1.0 milliliters, Tripolyglycerol monostearates 5 milligrams, ooze extracts liquid 1.5 milliliters, soil Extract 2.0 milliliters, ethylenediamine tetraacetic acid (EDTA) 6 milligrams, vitamins B
112 micrograms, vitamins B
121.5 micrograms, people urinates 2 milliliters, 1000 milliliters, the pure seawater after hypochlorite disinfectant.
15) a Platymonas helgolandica var cultural method, concrete steps are as follows:
Culture vessel is right cylinder polyethylene water tower, volume 3.0 cubic metres, and high-density polyethylene material is made (commercially available), colourless or white, there is good light transmission, carry out photosynthesis in order to algae, water tower is integral type structure, and wall thickness 6 millimeters, has very large intensity and soundness.Column head has a circular lid, above has and revolves silk, rotatable and seal.The base that an angle bar is welded into bottom water tower, high 50 centimetres.Column head lid diameter 40 centimetres, lid stamps 2 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, internal diameter 10 millimeters.Reactor is washed, rear distilled water rinse 3 times, load substratum according to claim 1 2000 liters, inflation mixing 5 minutes, select vigorous, the free of contamination Platymonas helgolandica var inoculation of vitality, inoculum density 2 × 10
4cell/mL; Lid inserts two clean Glass tubings, and one longer, 65 centimetres, 12 centimetres, lid is exposed on top, lower end leads to charge tube and is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone forms very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 30 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing, internal diameter 10 millimeters; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, and be filled with mixed gas, mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, can not float with frustule; Outside scenery, solar light irradiation, optimum temps 25 ± 2 DEG C, optimum illumination intensity 3000-10000Lux; First 5 days that cultivate, a simple and easy canopy is built above water tower, upper placement curtain shuts out the light, intensity of illumination is made to maintain 1500-2800Lux, avoid the light injury that sunburst causes cell, within 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and the synthesis of high unsaturated fatty acid; Be cultured to the 7th day, stop inflation, take away lid, supplement fresh culture 100 liters, cover lid, inflation, continue to cultivate, to the 9th day, Platymonas helgolandica var density was increased to 150 × 10
4cell/mL, stops cultivating, releases algae liquid, after being washed by reactor, add 0.2% peracetic acid disinfectant solution soaking and sterilize 2 hours, then use distilled water rinse one time from the water tap of bottom, after enter next round and cultivate;
Preferably, after being washed by culture vessel, do not adopt the disinfection way of " adding 0.2% peracetic acid disinfectant solution soaking to sterilize 2 hours ", and adopt ", add disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round cultivate, described Traditional Chinese medicine disinfectant is made up of the material of following weight ratio: the Chinese medicinal materials adding following weight part in every 100 part of 85% ethanol: Root of Indigowoad 30 ~ 50 parts, radix bupleuri 15 ~ 25 parts, peppermint 15 ~ 25 parts, cape jasmine 5 ~ 15 parts, tarragon 5 ~ 15 parts, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine fruit chopping, dry at 60-70 DEG C, 70 orders are crushed to respectively more for subsequent use with Chinese medicine material crushing machine, take respectively with table balance and dry and the Root of Indigowoad 120 grams pulverized, radix bupleuri 60 grams, mentha leave 60 grams, tarragon 30 grams, mountain Cape jasmine fruit 30 grams, after being placed in the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black agents bottle, labelled, be placed in 4 DEG C of refrigerators for subsequent use.", adopt important sterilization there is no chemical residue, more useful to the growth of algae.
Preferably, to the reactor described in selecting, carry out disinfection after washing, use ultraviolet lamp disinfection, sterilization 10-20 minute;
Preferably, to the reactor described in selecting, carry out disinfection after washing, use high pressure nitrogen to spray sterilization, sterilization 5-10 minute;
Preferably, to the reactor described in selecting, carry out disinfection after washing, use high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute;
Preferably, to the reactor described in selecting, point two sterilized phases after washing, first use high pressure nitrogen to spray sterilization, sterilization 2-3 minute; High-pressure carbon dioxide is used to spray sterilization, sterilization 2-3 minute; Algae culture is carried out again after sterilization.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (1)
1. utilize wastewater of starch factory to cultivate a substratum for Platymonas helgolandica var, it is characterized in that formula is as follows:
Wastewater of starch factory 75 milliliters, donkey excrement leach liquor 2.0 milliliters, rabbit excrement leach liquor 1.5 milliliters, sodium acetate 12 milligrams, 18 milligrams, ammonium sulfate, 24 milligrams, magnesium sulfate, 30 milligrams, agar, sucrose 25 milligrams, SODIUM PHOSPHATE, MONOBASIC 15 milligrams, propylene glycol alginate 10 milligrams, Sugaron 8 milligrams, Sorbitol Powder 1.0 milliliters, Tripolyglycerol monostearates 5 milligrams, ooze extracts liquid 1.5 milliliters, soil Extract 2.0 milliliters, ethylenediamine tetraacetic acid (EDTA) 6 milligrams, vitamins B
112 micrograms, vitamins B
121.5 micrograms, people urinates 2 milliliters, 1000 milliliters, the pure seawater after hypochlorite disinfectant.
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Effective date of registration: 20201208 Address after: No. 139, Jinchuan South Avenue, Xingan County, Ji'an City, Jiangxi Province Patentee after: Jiangxi kangshanyuan Oil Co.,Ltd. Address before: Room 1602, B1 building, Jinxiu Jiangnan (West Area), Gangli, east of Qingyun Road, north of Yinhe 2nd Road, Suzhou City, Anhui Province Patentee before: Anhui Huiteng Intellectual Property Co.,Ltd. Effective date of registration: 20201208 Address after: Room 1602, B1 building, Jinxiu Jiangnan (West Area), Gangli, east of Qingyun Road, north of Yinhe 2nd Road, Suzhou City, Anhui Province Patentee after: Anhui Huiteng Intellectual Property Co.,Ltd. Address before: 276000 Linyi University, middle ridge road, Shandong, Linyi Patentee before: LINYI University |
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Effective date of registration: 20231211 Address after: Yanhua City, dayangzhou Town, Xingan County, Ji'an City, Jiangxi Province Patentee after: Jiangxi Ganxing Gas Co.,Ltd. Address before: No. 139, Jinchuan South Avenue, Xingan County, Ji'an City, Jiangxi Province Patentee before: Jiangxi kangshanyuan Oil Co.,Ltd. |
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Effective date of registration: 20240603 Address after: No. 139, Jinchuan South Avenue, Xingan County, Ji'an City, Jiangxi Province Patentee after: Jiangxi kangshanyuan Oil Co.,Ltd. Country or region after: China Address before: Yanhua City, dayangzhou Town, Xingan County, Ji'an City, Jiangxi Province Patentee before: Jiangxi Ganxing Gas Co.,Ltd. Country or region before: China |