CN103969078A - Stool sample collecting device - Google Patents

Stool sample collecting device Download PDF

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Publication number
CN103969078A
CN103969078A CN201410176987.2A CN201410176987A CN103969078A CN 103969078 A CN103969078 A CN 103969078A CN 201410176987 A CN201410176987 A CN 201410176987A CN 103969078 A CN103969078 A CN 103969078A
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China
Prior art keywords
vessel
container
volume
fecal specimens
stool
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Pending
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CN201410176987.2A
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Chinese (zh)
Inventor
邹鸿志
傅新晖
吴珊
牛智通
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Guangzhou Kangliming Biological Science & Technology Co Ltd
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Individual
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Priority to CN201410176987.2A priority Critical patent/CN103969078A/en
Publication of CN103969078A publication Critical patent/CN103969078A/en
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Abstract

The invention relates to the technical field of medical instruments, and aims to provide a stool sample collecting device which is simple and reasonable in structure, can conveniently fulfill once complete collection for stool, and effectively solves the technical problem that stool molecule markers with lower abundance and high diagnostic values cannot be detected. The stool sample collecting device at least comprises a collecting container, and further comprises a protecting liquid container, wherein the collecting container comprises a container body provided with an opening and a container cover sealing the opening, the volume of the container body is 400 to 2000 ml; the protecting liquid container is used for containing a protecting liquid, and the volume of the protecting liquid container is 100 to 1000 ml. The stool sample collecting device has the advantages that a patient can excrete all stool into the collecting container, so that the stool is conveniently collected and then is sent to a molecule detecting laboratory to facilitate detection of the stool molecule markers. Therefore, the technical problem that the stool molecule markers with lower abundance and high diagnostic values cannot be detected is effectively solved.

Description

A kind of fecal specimens gathering-device
Technical field
The present invention relates to technical field of medical instruments, more specifically, relate to a kind of fecal specimens gathering-device.
Background technology
In prior art, it is one of routine clinical laboratory examination project that ight soil detects.Can understand more intuitively some pathological phenomenons of intestines and stomach by this inspection, indirectly judge the function status of alimentary canal, pancreas, liver and gall.At present, common ight soil detects and normally adopts gatherer from patient's ight soil, to collect sub-fraction sample to detect.Because the sample that this method is collected is very few, only can detect the albumen of flora and a few species, and the detection of other various types of molecular marked compounds (as patient DNA, RNA, miRNA, metabolin molecule and low-abundance protein etc.), because its abundance in ight soil is very low, the stool sample that uses existing fecal collecting device to collect cannot complete it and detect, and causes some abundance ight soil molecular marked compound lower but that have a height diagnostic value to be detected.Therefore, need one can carry out easily a large amount of collecting dungs, particularly the device of complete collecting dung once.
Summary of the invention
The present invention is intended to solve the problems of the technologies described above.
The object of this invention is to provide a kind of simple and reasonable, can complete easily once complete collecting dung, effectively solve some abundance lower but there is the fecal specimens gathering-device of the ight soil molecular marked compound technical matters that cannot be detected of height diagnostic value.
The technical solution adopted for the present invention to solve the technical problems is: a kind of fecal specimens gathering-device, at least comprise collection container, described collection container comprises the container cover that has the vessel of opening and opening is sealed, and the volume of described vessel is 400 ~ 2000 milliliters.
Generally, patient's once complete ight soil adds that to protect the volume range of liquid be 400 ~ 2000 milliliters.Therefore, in order to complete easily once the collection of complete ight soil, the volume of the vessel of collection container is designed to 400 ~ 2000 milliliters by the present invention, can guarantee that patient all comes ight soil in collection container, is convenient to ight soil to collect.Protect the volume of liquid to match because the volume of vessel and complete ight soil once add, can reduce as much as possible the occupied space of production cost and transport and storage.After patient's defecation is complete, container cover can be sealed to the opening of vessel, then deliver to Molecular Detection laboratory, to ight soil is carried out to molecular marked compound detection, effectively solve some abundance lower but have the technical matters that the ight soil molecular marked compound of height diagnostic value cannot be detected, simple and reasonable, production cost is low, easy to use, solve a difficult problem medically.
The present invention also comprises the protection liquid container for holding protection liquid, and the volume of described protection liquid container is 100 ~ 1000 milliliters.The present invention can support the use collection container and protection liquid container; complete after fecal specimens collection; the ight soil protection liquid that can maintain molecular marked compound biological stability in ight soil can be added to the vessel of collection container; then container cover is sealed to the opening of vessel, deliver to Molecular Detection laboratory and detect.Because the volume of the required protection liquid of complete fecal specimens is once 100 ~ 1000 milliliters; therefore the volume of protection liquid container is designed to 100 ~ 1000 milliliters by the present invention; to match with the volume of protection liquid; can either pack enough protection liquid into not have ight soil; reach the object of protection fecal specimens, can also reduce as much as possible the occupied space of production cost and transport and storage simultaneously.
In a preferred implementation, the openings of sizes of described vessel is 40 ~ 400 square centimeters.If the openings of sizes of vessel is less than 40 square centimeters, is not easy to patient ight soil is entered to vessel; If the openings of sizes of vessel is greater than 400 square centimeters, can increase the occupied space of production cost and transport and storage.
In a preferred implementation, the floorage size of described vessel is 40 ~ 400 square centimeters.If the floorage of vessel is less than 40 square centimeters, be under the prerequisite of a particular value at the volume of vessel, floorage is too small must be caused the excessive height of vessel or be a conical vessel wide at the top and narrow at the bottom, be not easy to it to place, also be not easy to patient ight soil is entered to vessel, and easily knocked over by patient; Because the area that ight soil occupies is limited, if the floorage of vessel is greater than 400 square centimeters, can cause occurring increasing equally the occupied space of production cost and transport and storage by larger vacant area in vessel.
In a preferred implementation, between described vessel and container cover, be provided with seal, described seal forms sealing between vessel and container cover.Complete after collecting dung, will between vessel and container cover, seal, with the disturbing factor that after reducing, experiment detects.Preferably, described seal is the soft edge strip of being located in vessel and/or container cover.Certainly, in other embodiments, also can adopt O-ring seal or other sealing means.
In one embodiment, between described vessel and container cover, adopt engagement connection or be threaded.Adopt engagement connection or the simplest connected mode such as be threaded, container cover can be sealed to the opening of vessel, without special design, production cost is low.
In one embodiment, the end face of described vessel is provided with the face of squatting of squatting for patient, is applicable to those patients taking toilet seat as main defecation mode.
Preferably, described in the face of squatting be the cancave cambered surface adapting with human buttock, patient takes more comfortablely like this, and has easier defecation compulsive patient for part.For example, vessel can be designed as the shape of toilet.
Preferably, described in squat face adopt soft plastic make, provide a comfortable sensation to patient equally.
The invention has the beneficial effects as follows:
1, creative on detected object: traditional ight soil detects the albumen that only detects flora and a few species, the ight soil that the present invention collects can be used for various types of molecular marked compounds and detects, as patient DNA, RNA, miRNA, metabolin molecule and low-abundance protein etc., these molecular marked compounds are because the abundance in ight soil is very low, use the stool sample that fecal collecting device was collected in the past cannot complete its detection, and the fecal sample that uses the present invention to collect can significantly improve the recall rate for above molecular marked compound.
2, creative in the application that detects disease type: traditional ight soil detects the detection that is mainly used in common intestines problem, although utilize the ight soil detection haemoglobin that classic method is collected to can be used for intestinal cancer examination, but sensitivity and specificity are all lower, and very low for the diagnostic value of early stage intestinal cancer.The ight soil that uses the present invention to collect can carry out downstream nucleic acid, albumen equimolecular label detects, and can greatly improve for early stage intestinal cancer, even the recall rate of adenoma.
3, creative in structural design: in order to complete easily once the collection of complete ight soil, the volume of the vessel of collection container is designed to 400 ~ 2000 milliliters by the present invention, can guarantee that patient all comes ight soil in collection container, is convenient to ight soil to collect.Protect the volume of liquid to match because the volume of vessel and complete ight soil once add, can reduce as much as possible the occupied space of production cost and transport and storage.After patient's defecation is complete, container cover can be sealed to the opening of vessel, then deliver to Molecular Detection laboratory, to ight soil is carried out to molecular marked compound detection, effectively solve some abundance lower but there is the ight soil molecular marked compound technical matters that cannot be detected of height diagnostic value;
The present invention simultaneously also comprises the protection liquid container for holding protection liquid; collection container and protection liquid container can be supported the use; complete after fecal specimens collection; the ight soil protection liquid that can maintain molecular marked compound biological stability in ight soil can be added to the vessel of collection container; then container cover is sealed to the opening of vessel, deliver to Molecular Detection laboratory and detect.Because the volume of the required protection liquid of complete fecal specimens is once 100 ~ 1000 milliliters; therefore the volume of protection liquid container is designed to 100 ~ 1000 milliliters by the present invention; to match with the volume of protection liquid; can either pack enough protection liquid into not have ight soil; reach the object of protection fecal specimens, can also reduce as much as possible the occupied space of production cost and transport and storage simultaneously.
Brief description of the drawings
Fig. 1 is that 123 parts of full fecal specimens of people add protection liquid final volume statistical graph.
Fig. 2 is the cross-sectional view of collection container in the embodiment of the present invention 1.
Fig. 3 is the decomposing schematic representation of Fig. 1.
Fig. 4 shows the data list that can hold ight soil and protect the ratio of liquid to change along with the volume of vessel.
Fig. 5 shows the change curve that can hold ight soil and protect the ratio of liquid to change along with the volume of vessel.
Fig. 6 shows patient's data list that accurately ratio of defecation changes along with the opening of vessel.
Fig. 7 shows patient's change curve that accurately ratio of defecation changes along with the opening of vessel.
Fig. 8 shows patient changes the careless disposable box ratio of doing to fall data list along with the floorage of vessel.
Fig. 9 shows patient changes the careless disposable box ratio of doing to fall change curve along with the floorage of vessel.
Figure 10 is that the full stool sample sample of single people is corresponding to the long-pending amplification curve result figure of difference protection liquid.
Figure 11 shows the data list that fluorescent value changes along with the volume of protection liquid.
Figure 12 shows the variation schematic diagram that fluorescent value changes along with the volume of protection liquid.
Figure 13 shows the data list that Ct value changes along with the volume of protection liquid.
Figure 14 shows the variation schematic diagram that Ct value changes along with the volume of protection liquid.
Figure 15 is the cross-sectional view of collection container in the embodiment of the present invention 2.
Figure 16 is the decomposing schematic representation of Figure 15.
Figure 17 is the cross-sectional view of collection container in the embodiment of the present invention 3.
Figure 18 is the decomposing schematic representation of Figure 17.
Figure 19 is the cross-sectional view of collection container in the embodiment of the present invention 4.
Figure 20 is the decomposing schematic representation of Figure 19.
Accompanying drawing, only for exemplary illustration, can not be interpreted as limitation of the present invention; For better explanation embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing some known features and explanation thereof may to omit be understandable.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further details.
Embodiment 1
As shown in Figure 1 to Figure 3, generally, patient's once complete ight soil adds that to protect the volume range of liquid be 400 ~ 2000 milliliters.Therefore, in order to complete easily once the collection of complete ight soil, the invention provides a kind of fecal specimens gathering-device, comprise collection container 100.Described collection container 100 comprises having the vessel 101 of opening and the container cover 102 that opening is sealed, and the volume of described vessel 101 is 400 ~ 2000 milliliters.
The volume of the vessel of collection container 100 101 is designed to 400 ~ 2000 milliliters by the present invention, and the collection container 100 of selecting different sizes for different patients, can guarantee that patient all comes ight soil in collection container 100, is convenient to ight soil to collect.Because the volume of vessel 101 and the volume of complete ight soil once match, can reduce as much as possible the occupied space of production cost and transport and storage.After patient's defecation is complete, container cover 102 can be sealed to the opening of vessel 101, then deliver to Molecular Detection laboratory, to ight soil is carried out to molecular marked compound detection, effectively solve some abundance lower but there is the ight soil molecular marked compound technical matters that cannot be detected of height diagnostic value.
The present invention also comprises the protection liquid container (not shown) for holding protection liquid, and the volume of described protection liquid container is 100 ~ 1000 milliliters.The present invention can support the use collection container 100 and protection liquid container; complete after fecal specimens collection; the ight soil protection liquid that can maintain molecular marked compound biological stability in ight soil can be added to the vessel 101 of collection container 100; then container cover 102 is sealed to the opening of vessel 101, deliver to Molecular Detection laboratory and detect.Because the volume of the required protection liquid of complete fecal specimens is once 100 ~ 1000 milliliters; therefore the volume of protection liquid container is designed to 100 ~ 1000 milliliters by the present invention; to match with the volume of protection liquid; can either pack enough protection liquid into not have ight soil; reach the object of protection fecal specimens, can also reduce as much as possible the occupied space of production cost and transport and storage simultaneously.
Protection liquid container in the present embodiment can be the various shapes such as bottle, box body, kettle body, as long as its volume scope reaches requirement.
In the present embodiment, between described vessel 101 and container cover 102, adopt engagement connection.In order to complete after collecting dung, by sealing between vessel 101 and container cover 102, with the disturbing factor that after reducing, experiment detects, between described vessel 101 and container cover 102, be provided with seal (not shown), described seal forms sealing between vessel 101 and container cover 102.In the present embodiment, described seal is the soft edge strip of being located in vessel 101 and/or container cover 102.Certainly, in other embodiments, also can adopt O-ring seal or other sealing means.
In the present embodiment, the openings of sizes of described vessel 101 is 40 ~ 400 square centimeters.If the openings of sizes of vessel 101 is less than 40 square centimeters, is not easy to patient ight soil is entered to vessel 101; If the openings of sizes of vessel 101 is greater than 400 square centimeters, can increase the occupied space of production cost and transport and storage.
In the present embodiment, the floorage size of described vessel 101 is 40 ~ 400 square centimeters.If the floorage of vessel 101 is less than 40 square centimeters, be under the prerequisite of a particular value at the volume of vessel 101, floorage is too small must be caused the excessive height of vessel 101 or be a conical vessel wide at the top and narrow at the bottom, be not easy to it to place, also be not easy to patient ight soil is entered to vessel, and easily knocked over by patient; Because the area that ight soil occupies is limited, if the floorage of vessel 101 is greater than 400 square centimeters, can cause the vacant area that the interior appearance of vessel 101 is larger, increase equally the occupied space of production cost and transport and storage.
Operation instruction of the present invention is: patient first drains urine, open the container cover 102 of the collection container 100 that is suitable for volume, squat above the vessel 101 of collection container 100, buttocks/anus is aimed at the opening of vessel 101, and whole ight soil is entered in vessel 101; Complete after defecation; protection liquid in protection liquid container is all poured in the vessel 101 of collection container; build the container cover 102 of collection container; so that collection container 100 is sealed; left and right horizontal is gently shaken 5-10 time, and testing laboratory is sent it to or delivered to the collection container 100 of the sealing that fecal specimens and protection liquid are housed.So far, fecal specimens has been collected.
It is below the data foundation of each numerical range in the present invention.
As shown in Figure 4, Figure 5, experimenter tests 10 experimenters, and experimenter's natural conditions such as age, health quite and have a ubiquity.When the volume of the vessel of collection container is 100,200,300,400,500,600 ... 2200 milliliters time, the average proportions that can hold ight soil and protection liquid is 22%, 40%, 61%, 80%, 91%, 100% ... 100%.Therefore, can all collect most experimenter's ight soil in order to ensure collection container, and avoid increasing considerably the cost of collection container, in conjunction with the 123 parts of full ight soil final volume of people statisticss of Fig. 1, select the volume scope of 400 to 2000 milliliters simultaneously.
As shown in Figure 6, Figure 7, experimenter tests 10 experimenters, and experimenter's natural conditions such as age, health quite and have a ubiquity.When the aperture area of the vessel of collection container is 20,30,40,60,80,100, when 120.......600 square centimeter, the ratio that patient can accurately enter ight soil collection container is 50%, 70%, 90%, 100%, 100%, 100%, 100% ... 100%.Therefore, in order to ensure most experimenter, whole ight soil is entered in collection container, avoid increasing considerably the cost of collection container simultaneously, select the opening scope of 40 to 400 square centimeters.
As shown in Figure 8, Figure 9, experimenter tests 10 experimenters, and experimenter's natural conditions such as age, health quite and have a ubiquity.When the floorage of the vessel of collection container is 20,30,40,60,80,100, when 120.....600 square centimeter, patient for example, is 80%, 60%, 30%, 10%, 0%, 0% by the careless ratio of doing to fall of collection container (being disposable box) ... 0%.Therefore, the stability while placement in order to ensure collection container avoids increasing considerably the cost of collection container simultaneously, selects the floorage scope of 40 to 400 square centimeters.
As shown in figure 10; in figure, each little figure is the full stool sample sample of single people corresponding to the long-pending amplification curve result figure of difference protection liquid, and wherein horizontal ordinate is Ct value, and ordinate is fluorescent value; curve represents to choose specific protection liquid when long-pending, corresponding change curve between fluorescent value and Ct value.
A figure to B figure is respectively the amplification curve result figure that when protection liquid is long-pending is less than 100ml, (choosing respectively 50ml, 90ml) draws, its Ct value (cycle threshold) is all greater than 28, and without amplification curve, fluorescence signal does not reach threshold value, is considered as invalid amplification; C figure to O figure is respectively protection liquid and amasss the amplification curve result figure that (chooses respectively 100ml, 150ml, 200ml, 250ml, 300ml, 400ml, 500ml, 600ml, 700ml, 800ml, 900ml, 950ml, 1000ml) at 100ml within the scope of 1000ml and draw, its Ct value (cycle threshold) is all less than 28, there is amplification curve, more than fluorescence signal all reaches threshold value, be considered as effective amplification; P figure to Q figure is that protection liquid amasss the amplification curve result figure that while being greater than 1000ml, (choosing respectively 1100ml, 1200ml) draws, its Ct value (cycle threshold) is all greater than 28, and fluorescence signal does not reach threshold value, is considered as invalid amplification.
As shown in Figure 11 to 14, choose respectively 50, 100, 200, 300 ... 1000, the protection liquid of 1100ml is long-pending, the protection liquid of every kind of volume is poured respectively into in 10 parts of full stool samples of people, (people's normal defecation weight is 150g-250g, 10 parts of sample weight choosing are all in usable range, all increase for β-actin gene in sample, β-actin gene is the house-keeping gene in biological cell, can not cause gene expression abundance to change because metamorphosis or living condition are different, conventionally in research purpose changes in gene expression, play the function of constant reference level), mix and carry out subsequent experimental detection, obtain 10 fluorescence numerical value, get its mean value and calculate SD value (standard deviation).Testing result is added up, Ct value (cycle threshold) be less than 28 and fluorescent value be considered as effective amplification higher than 700,000, otherwise be considered as invalid amplification.According to statistics, can draw once the required protection liquid of complete fecal specimens volume range be 100-1000ml.
Embodiment 2
The difference of the present embodiment and embodiment 1 is: in the present embodiment, between described vessel 101 and container cover 102, adopt and be threaded.As shown in Figure 4, Figure 5, between described vessel 101 and container cover 102, adopt external thread to be connected.Certainly, in other embodiments, between described vessel and container cover, also can adopt internal thread to be connected or other connected modes.
Other structures are identical with embodiment 1.
Embodiment 3
The difference of the present embodiment and embodiment 1 is: as shown in Figure 6, Figure 7, in the present embodiment, the end face of described vessel 101 is provided with the face of squatting 103 of squatting for patient, is applicable to those patients taking toilet seat as main defecation mode.
In the present embodiment, described in the face 103 of squatting adopt soft plastic to make, can provide a comfortable sensation to patient.
Other structures are identical with embodiment 1.
Embodiment 4
The difference of the present embodiment and embodiment 3 is: as shown in Figure 8, Figure 9, in the present embodiment, described face 103 cancave cambered surface for adapting with human buttock of squatting, like this patient take more comfortable, and for partly having compulsive patient easier defecation.For example, vessel 101 can be designed as the shape of toilet.
Other structures are identical with embodiment 3.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also giving exhaustive to all embodiments.All any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in the protection domain of the claims in the present invention.

Claims (9)

1. a fecal specimens gathering-device, is characterized in that,
At least comprise collection container, described collection container comprises the container cover that has the vessel of opening and opening is sealed, and the volume of described vessel is 400 ~ 2000 milliliters;
Also comprise the protection liquid container for holding protection liquid, the volume of described protection liquid container is 100 ~ 1000 milliliters.
2. fecal specimens gathering-device according to claim 1, is characterized in that, the openings of sizes of described vessel is 40 ~ 400 square centimeters.
3. fecal specimens gathering-device according to claim 1, is characterized in that, the floorage size of described vessel is 40 ~ 400 square centimeters.
4. according to the fecal specimens gathering-device described in claims 1 to 3 any one, it is characterized in that, between described vessel and container cover, be provided with seal, described seal forms sealing between vessel and container cover.
5. fecal specimens gathering-device according to claim 4, is characterized in that, described seal is the soft edge strip of being located in vessel and/or container cover.
6. fecal specimens gathering-device according to claim 4, is characterized in that, adopts engagement connection or be threaded between described vessel and container cover.
7. fecal specimens gathering-device according to claim 4, is characterized in that, the end face of described vessel is provided with the face of squatting of squatting for patient.
8. fecal specimens gathering-device according to claim 7, is characterized in that, described in the face of squatting be the cancave cambered surface adapting with human buttock.
9. fecal specimens gathering-device according to claim 7, is characterized in that, described in squat face adopt soft plastic make.
CN201410176987.2A 2014-04-29 2014-04-29 Stool sample collecting device Pending CN103969078A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104502138A (en) * 2014-12-20 2015-04-08 苏州承美生物科技有限公司 Faecal sample collector
CN106419711A (en) * 2016-12-28 2017-02-22 李天璞 Intelligent toilet lid for automatically detecting intestinal flora genes and application thereof

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CN203101087U (en) * 2013-03-28 2013-07-31 成都瑞琦科技实业有限责任公司 Disposable excrement specimen collecting cup
CN103439140A (en) * 2013-09-16 2013-12-11 汪建平 Excrement collection device suitable for detecting excrement molecular marker
CN103494571A (en) * 2013-10-11 2014-01-08 朱晓东 Multifunctional child pedestal pan
CN203798612U (en) * 2014-04-29 2014-08-27 汪建平 Excrement sample collection device

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Publication number Priority date Publication date Assignee Title
US20120117723A1 (en) * 2009-04-16 2012-05-17 Thomas Lloyd Commode
CN202397347U (en) * 2011-11-09 2012-08-29 嘉婴宝有限公司 Multifunctional combined bathroom product for children
CN202568035U (en) * 2012-03-31 2012-12-05 邬培英 Medical portable metering type pedestal pan
CN203101087U (en) * 2013-03-28 2013-07-31 成都瑞琦科技实业有限责任公司 Disposable excrement specimen collecting cup
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104502138A (en) * 2014-12-20 2015-04-08 苏州承美生物科技有限公司 Faecal sample collector
CN106419711A (en) * 2016-12-28 2017-02-22 李天璞 Intelligent toilet lid for automatically detecting intestinal flora genes and application thereof

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