CN103952474A - 一种食管癌诊断标志物及其使用方法 - Google Patents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
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- Proteomics, Peptides & Aminoacids (AREA)
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- Hospice & Palliative Care (AREA)
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- Oncology (AREA)
- Biochemistry (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
试剂 | 使用量 |
5×gDNA Eraser Buffer | 2.0μl |
gDNA Eraser | 1.0μl |
Total RNA | 1μg |
RNase Free dH2O | 加水至10μl |
试剂 | 使用量 |
步骤1的反应液 | 10.0μl |
PrimeScript RT Enzyme Mix I | 1.0μl |
RT Primer Mix | 1.0μl |
5×PrimeScript Buffer2 | 4μl |
RNase Free dH2O | 4.0μl |
总体积 | 20μl |
试剂 | 使用量 |
5×gDNA Eraser Buffer | 2.0μl |
gDNA Eraser | 1.0μl |
Total RNA | 1μg |
RNase Free dH2O | 加水至10μl |
试剂 | 使用量 |
步骤1的反应液 | 10.0μl |
PrimeScript RT Enzyme Mix I | 1.0μl |
RT Primer Mix | 1.0μl |
5×Prime Script Buffer2 | 4μl |
RNase Free dH2O | 4.0μl |
总体积 | 20μl |
试剂 | 使用量 |
5×gDNA Eraser Buffer | 2.0μl |
gDNA Eraser | 1.0μl |
Total RNA | 1μg |
RNase Free dH2O | 加水至10μl |
试剂 | 使用量 |
步骤1的反应液 | 10.0μl |
PrimeScript RT Enzyme Mix I | 1.0μl |
RT Primer Mix | 1.0μl |
5×Prime Script Buffer2 | 4μl |
RNase Free dH2O | 4.0μl |
总体积 | 20μl |
Claims (8)
Priority Applications (1)
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CN201410122636.3A CN103952474B (zh) | 2014-03-27 | 2014-03-27 | 一种食管癌诊断标志物及其使用方法 |
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CN201410122636.3A CN103952474B (zh) | 2014-03-27 | 2014-03-27 | 一种食管癌诊断标志物及其使用方法 |
Publications (2)
Publication Number | Publication Date |
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CN103952474A true CN103952474A (zh) | 2014-07-30 |
CN103952474B CN103952474B (zh) | 2018-05-15 |
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Country Status (1)
Country | Link |
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CN (1) | CN103952474B (zh) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104131108A (zh) * | 2014-08-13 | 2014-11-05 | 中国科学院上海微系统与信息技术研究所 | 一组人肺腺癌和人结直肠癌诊断用LncRNA生物标志物 |
CN104673797A (zh) * | 2015-02-09 | 2015-06-03 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
CN104877998A (zh) * | 2015-05-13 | 2015-09-02 | 中国人民解放军总医院 | 长链非编码rna以及检测细胞及组织中该长链非编码rna表达水平的引物对及试剂盒 |
CN105441447A (zh) * | 2015-02-09 | 2016-03-30 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
CN105441433A (zh) * | 2015-02-09 | 2016-03-30 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
CN105462974A (zh) * | 2015-02-09 | 2016-04-06 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
CN105886656A (zh) * | 2016-06-24 | 2016-08-24 | 河北医科大学第四医院 | Gif基因在食管鳞癌诊治中的应用 |
CN105969884A (zh) * | 2016-06-24 | 2016-09-28 | 河北医科大学第四医院 | Chia在食管鳞癌中的应用 |
CN106295246A (zh) * | 2016-08-07 | 2017-01-04 | 吉林大学 | 找到与肿瘤相关的lncRNA并预测其功能 |
CN111088357A (zh) * | 2019-12-31 | 2020-05-01 | 深圳大学 | 针对escc的肿瘤标志物及其应用 |
CN112359113A (zh) * | 2020-11-26 | 2021-02-12 | 中国人民解放军总医院第一医学中心 | LncRNA18q21.2在作为诊断食管癌的标志物中的应用、引物对和试剂盒 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102399889A (zh) * | 2011-12-01 | 2012-04-04 | 浙江大学 | 一种检测长链非编码rna-hotairm1的方法 |
CN103146693A (zh) * | 2013-02-26 | 2013-06-12 | 中南大学 | 一个长链非编码rna基因及其应用方法 |
-
2014
- 2014-03-27 CN CN201410122636.3A patent/CN103952474B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102399889A (zh) * | 2011-12-01 | 2012-04-04 | 浙江大学 | 一种检测长链非编码rna-hotairm1的方法 |
CN103146693A (zh) * | 2013-02-26 | 2013-06-12 | 中南大学 | 一个长链非编码rna基因及其应用方法 |
Non-Patent Citations (4)
Title |
---|
FEN-HWA WONG ET AL.: "Combination of microarray profiling and protein-protein interaction databases delineates the minimal discriminators as a metastasis network for esophageal squamous cell carcinoma", 《INTERNATIONAL JOURNAL OF ONCOLOGY》 * |
SUGANO S.ET AL.: "AK098705", 《GENBANK》 * |
谢海伟 等: "长链非编码RNA SPRY4-IT1在食管鳞癌中的表达及对细胞生长的影响", 《中国肿瘤临床》 * |
连贵勇 等: "长链非编码RNA HOTAIR 在食管鳞状细胞癌中的表达及意义", 《岭南现代临床外科》 * |
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104131108B (zh) * | 2014-08-13 | 2016-06-29 | 中国科学院上海微系统与信息技术研究所 | 一组人肺腺癌和人结直肠癌诊断用LncRNA 生物标志物 |
CN104131108A (zh) * | 2014-08-13 | 2014-11-05 | 中国科学院上海微系统与信息技术研究所 | 一组人肺腺癌和人结直肠癌诊断用LncRNA生物标志物 |
CN105441447B (zh) * | 2015-02-09 | 2018-05-29 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
CN104673797B (zh) * | 2015-02-09 | 2018-02-02 | 苏州大学 | 参与人体细胞电离辐射应激反应的长链非编码rna及其应用 |
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CN106295246A (zh) * | 2016-08-07 | 2017-01-04 | 吉林大学 | 找到与肿瘤相关的lncRNA并预测其功能 |
CN111088357A (zh) * | 2019-12-31 | 2020-05-01 | 深圳大学 | 针对escc的肿瘤标志物及其应用 |
CN111088357B (zh) * | 2019-12-31 | 2022-09-20 | 深圳大学 | 针对escc的肿瘤标志物及其应用 |
CN112359113A (zh) * | 2020-11-26 | 2021-02-12 | 中国人民解放军总医院第一医学中心 | LncRNA18q21.2在作为诊断食管癌的标志物中的应用、引物对和试剂盒 |
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