CN103951600A - Method for extracting beta-carotene from dunaliella salina - Google Patents
Method for extracting beta-carotene from dunaliella salina Download PDFInfo
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- CN103951600A CN103951600A CN201410203934.5A CN201410203934A CN103951600A CN 103951600 A CN103951600 A CN 103951600A CN 201410203934 A CN201410203934 A CN 201410203934A CN 103951600 A CN103951600 A CN 103951600A
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Abstract
The invention relates to a method for extracting beta-carotene from dunaliella salina. The method comprises the steps of (1) sufficiently mixing dunaliella salina paste or dunaliella salina powder and distilled water according to the mass ratio of 1:5, and regulating the pH value of the solution to 5-6.5 by using hydrochloric acid; (2) mixing protease and the dunaliella salina liquid according to the mass ratio of 1: (33.3-54.5), and then, sufficiently dissolving, preserving heat and passivating; (3) centrifuging an extracting solution, and then, taking out a sediment; (4) mixing the sediment and vegetable oil according to the mass ratio of 1:5, then, heating, keeping, standing, cooling and filtering to obtain an oil solution. According to the method provided by the invention, the effect of destroying cell membranes can be taken by adding a small deal of protease; the method is simple and convenient to operate, capable of saving the cost, suitable for large-scale industrial production, capable of omitting the process that oil suspension is needed after purification, environment-friendly, and capable of realizing large-scale popularization and application.
Description
Technical field
The invention belongs to technical field of food science, relate to particularly a kind of method of extracting β-carotene in salt algae.
Background technology
Salt algae (Dunaliella salina), belongs to Chlorophyta, Chlorophyceae, volvocales, Yan Zao section, Halophila, being a kind of unicellular Eukaryotic Algae, being distributed widely in the green alga in the higher areas of salinity such as ocean, salt lake, is one of eukaryote of the salt tolerant found up to now.Salt algae has very high economic worth, and under certain stress conditions, salt algae can produce and accumulate a large amount of β-carotenes, and its carotene carotene content can be up to 14% of dry cell weight.β-carotene is that country allows the foodstuff additive that use, there is painted and trophism, have simultaneously anti-oxidant, in conjunction with the nourishing functions such as active oxygen and medical functions, utilizing salt algae to produce carotene has become the important method of producing at present natural Beta-carotene both at home and abroad.
At present, the method for extracting β-carotene from salt algae has a lot, as organic solvent-extraction process, supercritical carbon dioxide extraction method etc.:
The comparatively popular institute of organic solvent extraction method is known, Applicative time is long, working method is easy, for example patent ZL95115709.4 has just introduced a kind of preparation method of natural Beta-carotene, and the method adopts edible organic solvent ethyl acetate purification by liquid extraction to prepare crystal carotene and concentrated solution medicinal extract product.This method is to have utilized β-carotene to be soluble in the characteristic of organic solvent, and operating process is easy, and quick, economy can extract β-carotene very rapidly from Dunaliella salina cell.But this method cause trouble can to the resource circulation utilization of algae-residue, β-carotene is generally as the raw material of functional foodstuff simultaneously, and in food, is not allow organic solvent residual.Have report to show, organic solvent, no matter be in the β-carotene extracting or in the functional substance such as polysaccharide from Dunaliella salina of subsequent extracted, can not be removed completely, and therefore, this method exists significant limitation.
Patent ZL200710120704.2 has introduced a kind of method that extracts carotenoid in Dunaliella salina, concrete steps are as follows: the Optimal culture of Dunaliella salina, tubular fibre embrane method concentrated algae liquid, centrifugation, dehydrate, the method for carotenoid in supercritical carbon dioxide extraction Dunaliella salina, this inventive method does not need to add any chemical reagent, environment is friendly, Dunaliella salina breaks in leaching process that rate of loss is low, carotenoid and the edible glycerol rate of recovery high, good product quality, non-environmental-pollution.But the carbon dioxide supercritical extraction method genus extra-high tension unit that arranges used, apparatus expensive, invests greatlyr, from Financial cost angle, considers, is unfavorable for the development of enterprise.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, and propose a kind of simple and feasiblely and no solvent residue, with proteolytic enzyme, extract the method for the β-carotene in salt algae.。
The present invention solves its technical problem and takes following technical scheme to realize:
Extract a method for the β-carotene in salt algae, it is characterized in that comprising the steps:
(1) salt algae mud or salt algae powder are fully mixed with the ratio of mass ratio 1:5 with distilled water, stir, with salt acid for adjusting pH value to 5-6.5;
(2) proteolytic enzyme is fully dissolved after mixing 1:33.3～54.5 in mass ratio with above-mentioned salt algae liquid; Be placed in 55-65 ℃ of insulation 1-1.5 hour, 85-95 ℃ of passivation 8-12 minute;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8-12min of 2500-3500r/min;
(4) precipitation is mixed to post-heating to 70-85 ℃ with vegetables oil with the ratio of mass ratio 1:5, keep standing cooling after 20-35 minute, filter to obtain oil solution.
And described proteolytic enzyme is a kind of in trypsinase, papoid or cellulase.
And described vegetables oil is one or more in Semen Maydis oil, raisin seed oil, Thistle oil or macadamia nut oil.
Advantage of the present invention and positively effect are:
(1) method of the present invention can utilize proteolytic enzyme to be hydrolyzed rapidly the outer cytolemma being comprised of glycoprotein and amino acid of Dunaliella salina cell, thereby protoplasma is come out.
(2) add a small amount of proteolytic enzyme and can play the effect that destroys cytolemma, easy and simple to handle, provide cost savings, be suitable for large-scale industrial production.
(3) β-carotene extracting is oil solution, saved the technique that needs to use again oil suspension after purifying, and extraction yield is high.
(4) present method environmental friendliness, can large-scale promotion be used.
Accompanying drawing explanation
Fig. 1 is process flow sheet of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the invention process is further described, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
Embodiment 1
(1) take fresh salt algae powder 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 6;
(2) in above-mentioned salt algae liquid, add the trypsinase of 2.2g, and fully dissolve; Be placed in 55 ℃ of insulations 1 hour, 85 ℃ of passivation 8 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8min of 2500r/min;
(4) get 6g precipitation and keep standing cooling after 25 minutes with 30g Semen Maydis oil mixing post-heating to 70 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 80%.
Embodiment 2
(1) take fresh salt algae mud 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 6;
(2) in above-mentioned salt algae liquid, add the trypsinase of 3.6g, and fully dissolve; Be placed in 55 ℃ of insulations 1 hour, 95 ℃ of passivation 8 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 12min of 3500r/min;
(4) get 6g precipitation and keep standing cooling after 25 minutes with 30g raisin seed oil mixing post-heating to 85 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 82%.
Embodiment 3
(1) take fresh salt algae powder 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 5.5;
(2) in above-mentioned salt algae liquid, add the papoid of 2.2g, and fully dissolve; Be placed in 60 ℃ of insulations 1.5 hours, 90 ℃ of passivation 12 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8min of 2500r/min;
(4) get 6g precipitation and keep standing cooling after 25 minutes with 30g Semen Maydis oil mixing post-heating to 70 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 81.5%.
Embodiment 4
(1) take fresh salt algae mud 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 5.5;
(2) in above-mentioned salt algae liquid, add the papoid of 3.6g, and fully dissolve; Be placed in 60 ℃ of insulations 1.5 hours, 90 ℃ of passivation 12 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 12min of 3500r/min;
(4) get 6g precipitation and keep standing cooling after 20 minutes with 30g Thistle oil mixing post-heating to 85 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 83%.
Embodiment 5
(1) take fresh algae powder 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 5;
(2) in above-mentioned salt algae liquid, add the cellulase of 2.2g, and fully dissolve; Be placed in 65 ℃ of insulations 1.5 hours, 95 ℃ of passivation 8 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8min of 2500r/min;
(4) get 6g precipitation and keep standing cooling after 35 minutes with 30g macadamia nut oil mixing post-heating to 75 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 80%.
Embodiment 6
(1) take fresh salt algae mud 20g, add 100g distilled water to stir, with salt acid for adjusting pH value to 5;
(2) in above-mentioned salt algae liquid, add the cellulase of 3.6g, and fully dissolve; Be placed in 65 ℃ of insulations 1.5 hours, 95 ℃ of passivation 8 minutes;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8min of 2500r/min;
(4) get 6g precipitation and keep standing cooling after 25 minutes with 30g Semen Maydis oil mixing post-heating to 70 ℃, filter to obtain oil solution;
After testing: β-carotene extraction yield is 81%.
Claims (3)
1. extract a method for the β-carotene in salt algae, it is characterized in that comprising the steps:
(1) salt algae mud or salt algae powder are fully mixed with the ratio of mass ratio 1:5 with distilled water, stir, with salt acid for adjusting pH value to 5-6.5;
(2) proteolytic enzyme is fully dissolved after mixing 1:33.3～54.5 in mass ratio with above-mentioned salt algae liquid; Be placed in 55-65 ℃ of insulation 1-1.5 hour, 85-95 ℃ of passivation 8-12 minute;
(3) extracting solution of step (2) is got to precipitation after the centrifugal 8-12min of 2500-3500r/min;
(4) precipitation is mixed to post-heating to 70-85 ℃ with vegetables oil with the ratio of mass ratio 1:5, keep standing cooling after 20-35 minute, filter to obtain oil solution.
2. the method for the β-carotene in extraction salt algae according to claim 1, is characterized in that described proteolytic enzyme is a kind of in trypsinase, papoid or cellulase.
3. the method for the β-carotene in extraction salt algae according to claim 1, is characterized in that described vegetables oil is one or more in Semen Maydis oil, raisin seed oil, Thistle oil or macadamia nut oil.
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| CN201410203934.5A CN103951600A (en) | 2014-05-15 | 2014-05-15 | Method for extracting beta-carotene from dunaliella salina |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104367572A (en) * | 2014-10-22 | 2015-02-25 | 中盐工程技术研究院有限公司 | Natural dunaliella salina beta-carotene vitamin E soft capsule |
| CN109322180A (en) * | 2018-09-11 | 2019-02-12 | 泉州市缘创环保科技有限公司 | A kind of colouring method of natural salt algae beta carotene |
Citations (4)
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|---|---|---|---|---|
| CN1059716A (en) * | 1991-07-29 | 1992-03-25 | 天津市房地产开发经营集团塘沽总公司 | The method of leaching carotene from the salt algae |
| CN1084848A (en) * | 1992-09-29 | 1994-04-06 | 南京师范大学 | The collection of salt algae and the extracting method of β-Hu Luobusu |
| CN1429544A (en) * | 2003-01-13 | 2003-07-16 | 内蒙古吉兰泰盐化集团呼和浩特制药厂 | Natural carotene soft capsule and its production method |
| US20040253227A1 (en) * | 2002-09-11 | 2004-12-16 | Martin Kenneth A. | Perioperative multivitamin protein beverage and additive for use in preparing an individual for fast surgical recovery |
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2014
- 2014-05-15 CN CN201410203934.5A patent/CN103951600A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059716A (en) * | 1991-07-29 | 1992-03-25 | 天津市房地产开发经营集团塘沽总公司 | The method of leaching carotene from the salt algae |
| CN1084848A (en) * | 1992-09-29 | 1994-04-06 | 南京师范大学 | The collection of salt algae and the extracting method of β-Hu Luobusu |
| US20040253227A1 (en) * | 2002-09-11 | 2004-12-16 | Martin Kenneth A. | Perioperative multivitamin protein beverage and additive for use in preparing an individual for fast surgical recovery |
| CN1429544A (en) * | 2003-01-13 | 2003-07-16 | 内蒙古吉兰泰盐化集团呼和浩特制药厂 | Natural carotene soft capsule and its production method |
Non-Patent Citations (3)
| Title |
|---|
| 徐怀德: "《天然产物提取工艺学》", 31 December 2006 * |
| 石斌，石军: "利用盐生杜氏藻提取β－胡萝卜素", 《精细化工》 * |
| 赵大球等: "植物类胡萝卜素分析方法研究进展", 《北方园艺》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104367572A (en) * | 2014-10-22 | 2015-02-25 | 中盐工程技术研究院有限公司 | Natural dunaliella salina beta-carotene vitamin E soft capsule |
| CN109322180A (en) * | 2018-09-11 | 2019-02-12 | 泉州市缘创环保科技有限公司 | A kind of colouring method of natural salt algae beta carotene |
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Application publication date: 20140730 |