CN103949116B - A kind of can as the gel combined filtration of microbe carrier continuous and preparation method - Google Patents
A kind of can as the gel combined filtration of microbe carrier continuous and preparation method Download PDFInfo
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- CN103949116B CN103949116B CN201410170007.8A CN201410170007A CN103949116B CN 103949116 B CN103949116 B CN 103949116B CN 201410170007 A CN201410170007 A CN 201410170007A CN 103949116 B CN103949116 B CN 103949116B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention belongs to and filter continuous technical field, particularly relate to a kind of can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, the tender hydrogel of porous forms the net line structure interted mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5% ~ 50%, and the small aperture in porous silk floss is 1 μm ~ 1cm.Gel combined filtration of the present invention is continuous to be passed through organically to be combined into based on the mode layer by layer from cross-linking by the tender gel bi-material of porous, gel network is interted to add between the space of sponge and is carried a bacterium capacity, and porous is continuous provides the additional channels of mechanical support and delivering oxygen, nutrient for gel layer, simultaneously because the aperture of gel layer is little compared with the small aperture in porous silk floss, also improve filter effect.
Description
Technical field
The invention belongs to and filter continuous technical field, particularly relate to a kind of can as the gel combined filtration of microbe carrier continuous and preparation method.
Background technology
Immobilized microorganism technique is an emerging technology in modern biological project field, it be by the means of chemistry or physics by free cell, microorganism or electrodes method in the area of space limited, make it keep active and the method that can recycle.Microorganism is mainly employed in a free form before this.But free microorganism is easily washed away by current under the flow conditions, under hydrostatic condition easily eat by other biological, and when lacking absorption and the carrier of growth and breeding, it is difficult to stably play function for a long time.Therefore people start to consider to utilize immobilized microorganism technique to replace traditional method of shedding free bacteria in water treatment field.The former comparatively the latter have obvious superiority, be mainly manifested in: treatment effeciency is high, stability is strong, can purifying and keep that high-efficiency strain, microorganism concn are high, good effect of separating solid from liquid.
Immobilization method of microorganism, mainly contains the several method such as adsorption (combination) technique for fixing, crosslinked (binding) immobilization, embedding (polymer) immobilization and self immobilization (between microbial cell self-crosslinking immobilization).In various microorganism immobilization method, investment is low with its density, be easy to fluidised superiority becomes the most widely used method of research at present.The carrier material of conventional investment fixation of microbe, has the agar of bio-based high molecular polysaccharide, sodium alginate, carragheen and synthetic macromolecular compound polyacrylamide (PACAM), polyvinyl alcohol (PVA).Wherein, bio-based high-molecular gel is as sodium alginate because the process of its gel forming is simple, and mild condition, does not have toxicity to microbial cell, so it is the most extensive in the application of the embedding of microbial cell.But investment complicated process of preparation, preparation cost are high, material mechanical performance is poor, resistance to mass tranfer is large, larger to the metabolic effect of microbial growth and product.And at present on the market the conventional tender sponge of biochemistry that is raw material with polyurethane, polyethers except filtration except, also all have the effect of absorption fixation of microbe concurrently, but the capacity of year bacterium is very little.
Summary of the invention
The object of the invention is to provide for the deficiencies in the prior art a kind of can be continuous as the gel combined filtration of microbe carrier.
For achieving the above object, the present invention adopts following technical scheme: a kind of can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, the tender hydrogel of porous forms the net line structure interted mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5% ~ 50%, and the small aperture in porous silk floss is 1 μm ~ 1cm.
Porous silk floss is polyurethanes sponge, the biochemical continuous or biochemical silk floss of polyesters of polyethers.
The beneficial effect of gel combined filtration silk floss of the present invention, gel combined filtration of the present invention is continuous to be passed through organically to be combined into based on the mode layer by layer from cross-linking by the tender gel bi-material of porous, gel network is interted to add between the space of sponge and is carried a bacterium capacity, and porous is continuous provides the additional channels of mechanical support and delivering oxygen, nutrient for gel layer, simultaneously because the aperture of gel layer is little compared with the small aperture in porous silk floss, also improve state's filter effect.
The present invention also provide a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, its preparation process comprises:
The first step, immerses plastic Polymer Solution 1 ~ 10 minute by porous silk floss;
Second step, puts into the porous of soaking through first step silk floss and solidifies solution or under the cryogenic conditions of-20 DEG C ~ 0 DEG C, form gel combined filtration silk floss;
3rd step, immerses microbial solution, standing adsorption 0.5 ~ 5 hour by gel combined filtration silk floss obtained for second step, obtained finished product.
Plastic Polymer Solution is the composite solution of sodium alginate, agar-agar and polyvinyl alcohol mixing, and wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5% ~ 20%.
Solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
Microbial solution includes aerobic microbiological, and aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the aerobic microbiological mass fraction accounted in microbial solution is 1% ~ 30%.
The present invention also provides another kind can as the preparation method of the gel combined filtration silk floss of microbe carrier, and its preparation process comprises:
The first step, is mixed to form microbiological gum liquid solution by plastic Polymer Solution and microbial solution equal-volume;
Second step, immerses the obtained microbiological gum liquid solution of the first step 1 ~ 10 minute by porous silk floss;
3rd step, puts into the porous silk floss through second step process and solidifies solution or obtained finished product under the cryogenic conditions of-20 DEG C ~ 0 DEG C.
Also comprise the 4th step, continuous through the porous of the 3rd step process by clean water, wash away the solution of ungelled part, then porous silk floss is immersed and solidify solution 1 ~ 10 minute, again tender for the porous solution that solidifies is put into-20 DEG C ~ 0 DEG C low temperature environment together, make to solidify solution gel.
Plastic Polymer Solution is the composite solution of sodium alginate, agar-agar and polyvinyl alcohol mixing, and wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5% ~ 20%.
Solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
Microbial solution includes aerobic microbiological, and aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the aerobic microbiological mass fraction accounted in microbial solution is 1% ~ 30%.
The beneficial effect of preparation method of the present invention: preparation method of the present invention is simple to operate, obtained gel combined filtration silk floss adds and carries bacterium capacity, and porous is continuous provides the additional channels of mechanical support and delivering oxygen, nutrient for gel layer, simultaneously because the aperture of gel layer is little compared with the small aperture in porous silk floss, also improve state's filter effect.
Detailed description of the invention
A kind of can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, the tender hydrogel of porous forms the net line structure interted mutually, and the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5% ~ 50%, and the small aperture in porous silk floss is 1 μm ~ 1cm.
Porous silk floss is polyurethanes sponge, the biochemical continuous or biochemical silk floss of polyesters of polyethers.
Embodiment 1
The preparation of agar-agar gel composite filter silk floss: take polyurethane sponge as base material (market is bought), its voidage is 60%, and average pore size is 2mm, and thickness is 8cm.Preparation process:
The first step, immerses in the agar-agar solution of 90 DEG C 10% by the sponge of polyurethane sponge bottom about 3cm, leaves standstill 3 minutes;
Second step, puts into the refrigerator of 0 DEG C of degree, places 2 hours cooled and solidified plastics together with agar-agar solution by the polyurethane sponge of the first step;
3rd step, puts into the bacterium original solution of the photosynthetic bacteria containing 20% mass fraction by the polyurethane sponge through second step process, place and allow microbe passive adsorb in 1 hour, and the gel filter silk floss being loaded with compound bacteria so has just been made.
Embodiment 2
The preparation of polyvinyl alcohol gel composite filter silk floss: think that polyurethane sponge is base material (market is bought), its voidage is 70%, and average pore size is 2mm, and thickness is 8cm.Preparation process:
The first step, immerses in polyvinyl alcohol (trade mark 1799) solution of 90 DEG C 15% by the sponge of polyurethane sponge bottom about 3cm, leave standstill 3 minutes;
Second step, puts into the refrigerator of-20 DEG C together with poly-vinyl alcohol solution by the polyurethane sponge of the first step, place 10 hours cooled and solidified plastics;
3rd step, puts into the bacterium original solution of the photosynthetic bacteria containing 20% mass fraction by the polyurethane sponge through second step process, place and allow microbe passive adsorb in 1 hour, and the gel filter silk floss being loaded with the polyvinyl alcohol of compound bacteria so has just been made.
Embodiment 3
The preparation method of calcium alginate gel combined filtration silk floss: biochemical continuous for base material (market is bought) with polyesters, its voidage is 75%, and average pore size is 1mm, and thickness is 5cm.Preparation process:
The first step, is mixed into microbiological gum liquid solution by the solution equal-volume containing the sodium alginate gelling solution of 4% and the former bacterium of nitrobacteria of 10%;
Second step, immerses the sponge of biochemical for polyesters continuous bottom about 2cm in the obtained microbiological gum liquid solution of the first step, stops 5 minutes;
3rd step, the biochemical silk floss of polyesters through second step process is put into again the calcium chloride solution of 2%, leave standstill and solidify plastic in 3 minutes, clean water gel surface is used after plastic, and then repeat the above-mentioned first step to the 3rd step plastic operation twice, make gel layer thicknesses reach pre-provisioning request, carry germy gel combined filtration silk floss like this and just made.
Embodiment 4
The preparation of calcium alginate-polyvinyl alcohol gel composite filter silk floss: biochemical continuous for base material (market is bought) with polyesters, its voidage is 75%, and average pore size is 2mm, and thickness is 10cm.Preparation process:
The first step, is mixed into microbiological gum liquid solution by containing the sodium alginate gelling solution of 5% and the nitrobacteria solution equal-volume of 10%;
Second step, injects the biochemical silk floss of polyesters with syringe by microbiological gum liquid solution obtained for this first step, makes microbiological gum liquid solution be evenly distributed on the biochemical 2cm place, continuous bottom of polyesters, make microbiological gum liquid solution formed thickness be 1cm contain glue thin layer;
3rd step, puts into the calcium gluconate solution of 2%, leaves standstill and solidify plastic in 5 minutes by the biochemical silk floss of polyesters through second step process;
4th step, biochemical continuous through the polyesters of the 3rd step process by clean water, wash away the solution of ungelled part, then the sponge at biochemical for polyesters 4cm place, continuous bottom is all immersed in polyvinyl alcohol (trade mark 1799) solution of 40 DEG C 8%, stop 10 minutes, finally put into-20 DEG C of refrigerators 2 hours, make poly-vinyl alcohol solution gel, the gel filter silk floss being loaded with the calcium alginate-polyvinyl alcohol of compound bacteria so has just been made.
The announcement of book and instruction according to the above description, those skilled in the art in the invention can also change above-mentioned embodiment and revise.Therefore, the present invention is not limited to above-mentioned detailed description of the invention, and any apparent improvement of every those skilled in the art done by basis of the present invention, replacement or modification all belong to protection scope of the present invention.In addition, although employ some specific terms in this description, these terms just for convenience of description, do not form any restriction to the present invention.
Claims (10)
1. one kind can as the preparation method of the gel combined filtration silk floss of microbe carrier, gel combined filtration silk floss comprises the tender hydrogel of porous, the tender hydrogel of porous forms the net line structure interted mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5% ~ 50%, and the small aperture in porous silk floss is 1 μm ~ 1cm; Its preparation process comprises:
The first step, immerses plastic Polymer Solution 1 ~ 10 minute by porous silk floss;
Second step, puts into the porous of soaking through first step silk floss and solidifies solution or under the cryogenic conditions of-20 DEG C ~ 0 DEG C, form gel combined filtration silk floss;
3rd step, immerses microbial solution, standing adsorption 0.5 ~ 5 hour by gel combined filtration silk floss obtained for second step, obtained finished product.
2. according to claim 1 a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: plastic Polymer Solution is the composite solution of sodium alginate, agar-agar and polyvinyl alcohol mixing, wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5% ~ 20%.
3. according to claim 1ly a kind ofly as the preparation method of the gel combined filtration silk floss of microbe carrier, can to it is characterized in that: solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
4. according to claim 1 a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: microbial solution includes aerobic microbiological, aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the aerobic microbiological mass fraction accounted in microbial solution is 1% ~ 30%.
5. a kind of according to Claims 1-4 any one as the preparation method of the gel combined filtration silk floss of microbe carrier, can is characterized in that: porous silk floss is polyurethanes sponge, the biochemical continuous or biochemical silk floss of polyesters of polyethers.
6. one kind can as the preparation method of the gel combined filtration silk floss of microbe carrier, gel combined filtration silk floss comprises the tender hydrogel of porous, the tender hydrogel of porous forms the net line structure interted mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5% ~ 50%, and the small aperture in porous silk floss is 1 μm ~ 1cm; Its preparation process comprises:
The first step, is mixed to form microbiological gum liquid solution by plastic Polymer Solution and microbial solution equal-volume;
Second step, immerses the obtained microbiological gum liquid solution of the first step 1 ~ 10 minute by porous silk floss;
3rd step, puts into the porous silk floss through second step process and solidifies solution or obtained finished product under the cryogenic conditions of-20 DEG C ~ 0 DEG C.
7. according to claim 6 a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: also comprise the 4th step, continuous through the porous of the 3rd step process by clean water, wash away the solution of ungelled part, then porous silk floss is immersed and solidify solution 1 ~ 10 minute, again tender for the porous solution that solidifies is put into-20 DEG C ~ 0 DEG C low temperature environment together, make to solidify solution gel.
8. according to claim 6 a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: plastic Polymer Solution is the composite solution of sodium alginate, agar-agar and polyvinyl alcohol mixing, wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5% ~ 20%.
9. according to claim 6 a kind of can as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: microbial solution includes aerobic microbiological, aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the aerobic microbiological mass fraction accounted in microbial solution is 1% ~ 30%.
10. a kind of according to claim 6 to 9 any one as the preparation method of the gel combined filtration silk floss of microbe carrier, can is characterized in that: porous silk floss is polyurethanes sponge, the biochemical continuous or biochemical silk floss of polyesters of polyethers.
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CN104774830B (en) * | 2015-03-27 | 2018-05-08 | 成都百特万合医药科技有限公司 | A kind of process for fixation of transaminase |
TR201807916A2 (en) * | 2018-06-04 | 2018-06-21 | Filkim Filtre Ve Kimya San Tic A S | ULTRA HIGH EFFICIENCY ORGANIC GEL MICROBIAL AIR FILTRATION AND PRODUCTION SYSTEM |
CN109351055A (en) * | 2018-10-24 | 2019-02-19 | 武汉大学 | A kind of hydrate gel type filter core and preparation method thereof |
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CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
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CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
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Effective date of registration: 20200113 Address after: 315500 Dongfeng Road, Fenghua District, Ningbo, Zhejiang Province, No. 80 Patentee after: Ningbo reputation Environment Technology Co., Ltd. Address before: 7, building 7006, 1558 Jiangnan Road, 315048 hi tech Zone, Zhejiang, Ningbo Patentee before: NINGBO SUNNY NOVEL MATERIAL TECHNOLOGY CO., LTD. |
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