CN106450398B - The method for quickly establishing microbiological fuel cell nitrification - Google Patents
The method for quickly establishing microbiological fuel cell nitrification Download PDFInfo
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- CN106450398B CN106450398B CN201610873455.3A CN201610873455A CN106450398B CN 106450398 B CN106450398 B CN 106450398B CN 201610873455 A CN201610873455 A CN 201610873455A CN 106450398 B CN106450398 B CN 106450398B
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- fuel cell
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/02—Details
- H01M8/0202—Collectors; Separators, e.g. bipolar separators; Interconnectors
- H01M8/023—Porous and characterised by the material
- H01M8/0239—Organic resins; Organic polymers
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The present invention relates to new energy and sewage treatment field, it is desirable to provide a method of quickly establish microbiological fuel cell nitrification.Include: to handle diaphragm material, diaphragm material surface is made to form the biotic environment that nitrobacteria is easy to grow film forming；The solution containing nitrobacteria is run with gained microbiological fuel cell, for realizing the inoculation of nitrobacteria, forms nitrobacteria biomembrane on diaphragm material.The present invention can greatly shorten the settling time of microbiological fuel cell nitrification；During establishing microbiological fuel cell nitrification, denitrifying bacteria also synchronous growth may be implemented that synchronous nitration denitrification process occurs in reactor；Easy to operate using cheap diaphragm as the attachment site of nitrobacteria, treatment effect is stablized, and is conducive to microbiological fuel cell and obtains electricity generation performance steady in a long-term, is very beneficial for practical application.
Description
Technical field
The present invention relates to a kind of methods for quickly establishing microbiological fuel cell nitrification, belong at new energy and sewage
Reason field.
Background technique
Currently, being two step bioanalysis of nitration denitrification in the most important denitrogenation technology in city or Industrial Waste Water Treatments factory, i.e.,
Ammonia nitrogen occurs oxidation reaction first under the action of nitrobacteria and generates nitrate nitrogen, and then nitrate nitrogen occurs under the action of denitrifying bacterium
Reduction reaction generates nitrogen, to realize the removing of ammonia nitrogen in waste water.This traditional biological denitrogenation technology can be with efficient stable
Removal of ammonia and nitrogen, but have the shortcomings that starting time long (it is generally necessary to 30-60 days), processing cost are high, it is therefore desirable to it develops new
The more denitrogenation technology of economy.
Microbiological fuel cell (Microbial Fuel Cell, hereinafter referred to as MFC) is one and utilizes anaerobism or facultative micro-
The chemical energy being stored in organic matter is converted into the new technology of electric energy by biology.When using nitrogenous organic wastewater as matrix
When, MFC can be produced electricl energy while degrading COD and ammonia nitrogen, had the advantages that high treating effect, processing cost are low, located
There is huge application potential in terms of managing nitrogenous effluent.However, establishing mature nitrification in microbiological fuel cell still
Long period (2-3 months) are needed, its practical application is limited.
Nitrobacteria bears the generation of nitration reaction, is mostly aerobic autotrophic type bacterium, the speed of growth is far below different
Type bacterium is supported, therefore the foundation of nitrification needs the long period mostly in mixed cell system.Nitrobacteria is in attachment material
Attachment film forming on material is the key that nitrifying process is established, its attachment film forming is closely related with the surface nature of attachment material.
In the bioreactor of non-microorganism fuel cell, the surface area of attachment material is modified or increased to attachment material surface
It can accelerate the growth and film forming of nitrifier, shorten nitrification settling time.In single chamber without diaphragm air cathode microbial fuel cell
In, cathode surface is the main attachment site of nitrobacteria.Currently, binder using polyethyene diamine as cathode catalysis layer is repaired
Decorations cathode surface simultaneously increase cathodic surface area it has been reported that but result of study show this method to microbiological fuel cell nitre
The foundation of change system does not help, and the time for establishing nitrification, there is no shortenings, and polyethyene diamine is a kind of toxic and readily soluble
In the high molecular polymer of water, the secondary pollution of environment will cause in use, therefore this method is difficult to meet reality
The requirement of application.
The specific surface area for increasing nitrobacteria attachment material is to speed up another method of nitrification foundation.In single chamber sky
In gas cathode microbial fuel cell, prevent the biology of two electric pole short circuit of yin-yang and cathod catalyst dirty usually using diaphragm material
The electricity generation performance of microbiological fuel cell is contaminated and influences, therefore diaphragm can be at the main attachment site of nitrobacteria.Diaphragm material
Characteristic with high porosity, high-specific surface area is conducive to the fast-growth of nitrobacteria, but the poor biocompatibility meeting of diaphragm
Nitrifier is influenced in the attachment on its surface.Therefore, how to improve the biocompatibility of diaphragm material in microbiological fuel cell
It is the key that quickly to establish nitrification and realize that the technology is practical in terms of handling nitrogenous effluent
Summary of the invention
The technical problem to be solved by the present invention is to when overcoming microbiological fuel cell processing nitrogen-containing wastewater in the prior art
The problem for establishing nitrification time length provides the simple diaphragm material surface biological environment that improves of one kind and quickly establishes nitrification
The method of bacterial biof iotalm.This method can effectively shorten nitrification settling time, not need complicated equipment and operation, and not
It will cause environmental pollution.
In order to solve the technical problem, inventive solution is:
A kind of method for quickly establishing microbiological fuel cell nitrification is provided, comprising the following steps:
(1) diaphragm material is handled, diaphragm material surface is made to form the biocycle that nitrobacteria is easy to grow film forming
Border；Specific steps are as follows:
Diaphragm material is cut into size identical with cathode, is placed on the inside of air cathode Catalytic Layer, with carbon brush anode unit
At having septate microbiological fuel cell, it is subsequently filled electricity production microbionation liquid；15~40 DEG C at a temperature of, use is external
Electric-resistivity method starts the microbiological fuel cell；After starting successfully, which is run with the phosphate buffer containing organic carbon source
Object fuel cell, it is every to be for 24 hours a cycle of operation and replace solution, until microbiological fuel cell obtains stable electricity production
Can, the biotic environment for being easy to nitrobacteria growth film forming is formed on diaphragm material at this time；
(2) solution containing nitrobacteria is run with microbiological fuel cell obtained by previous step, it is thin for realizing nitrification
The inoculation of bacterium forms nitrobacteria biomembrane on diaphragm material；Specific steps are as follows:
Use external several periods of electric-resistivity method continuous operation to establish MFC nitrification, every be a cycle of operation for 24 hours
And replace the solution containing nitrobacteria；When the ammonia-nitrogen content of microbiological fuel cell water outlet is lower than 5mg/L, and continuous two week
When phase water outlet ammonia nitrogen keeps stablizing, then it is assumed that form stable nitration reaction in battery, formed nitre on diaphragm material at this time
Change bacterial biof iotalm, microbiological fuel cell nitrification has been established.
In the present invention, the diaphragm material is any one following: glass mat, glass fabric, nylon every
Film, carbon cloth, carbon paper, carbon felt or macromolecule cloth.
In the present invention, the microbiological fuel cell structure is any one following: double air cathode single-chamber microbials
Fuel cell, single air cathode single-chamber microbial fuel cell or air cathode double-chamber microbiological fuel cell.
In the present invention, the outer connecting resistance used in microbiological fuel cell starting and nitrification establishment process, electricity
Resistance value is 7000-20000 Ω/cm according to the area setting resistance of air cathode2Cathode area is (for example, cathode area is 7cm2When,
Outer connecting resistance uses 1000 Ω, when cathode area is 200cm2When, outer connecting resistance uses 100 Ω).
In the present invention, the electricity production microbionation liquid is any one following: long-term running air single chamber cathode
Microbiological fuel cell water outlet, city domestic sewage treatment plant primary sedimentation tank water, anaerobic activated sludge or aerobic activated sludge.
In the present invention, the phosphate buffer containing organic carbon source refers to: using sodium acetate as the phosphoric acid of organic carbon source
Salt buffer；According to the difference of service condition, sodium acetate concentration is set as 1~5g/L, phosphate buffer is 10~250mM.
In the present invention, the solution containing nitrobacteria is any one following: slaughterhouse wastewater treatment factory primary sedimentation tank
Water, aerobic activated sludge, the water outlet of cultivating wastewater purification factory nitrification tank or the water outlet of nitrogen-containing wastewater treatment plant nitrification tank.
Inventive principle description:
The present invention is carried out in two steps:
The first step provides a kind of simple and environmentally-friendly diaphragm material processing method, so that diaphragm material surface is formed nitrobacteria easy
In the biotic environment of growth film forming comprising the steps of: diaphragm material is cut into size identical with cathode, is placed in air
On the inside of cathode catalysis layer, there is diaphragm air cathode microbial fuel cell with carbon brush anode composition.Under experimental temperature, using containing
The microbiological fuel cell for having the inoculation liquid such as laboratory of electricity production bacterium to run steadily in the long term pours out liquid using external electric-resistivity method
Start the microbiological fuel cell.After starting successfully, which runs the phosphate-buffered containing organic carbon source
Liquid, battery each cycle of operation are for 24 hours.Microbiological fuel cell it is stable i.e. obtain stable electricity generation performance after, this after
The biotic environment for being easy to nitrobacteria growth film forming is formed on film.
Second step provides a kind of simple and quick method for establishing nitrification, comprising the following steps: above-mentioned electricity production is stable
Microbiological fuel cell runs the inoculation that such as slaughtering wastewater of the solution containing nitrobacteria carries out nitrobacteria, suitable external
Several periods (each cycle 24 hours) of continuous operation establish nitrification under resistance, form in a short time on diaphragm at this time
Mature nitrobacteria biomembrane.
The invention has the following advantages:
(1) it is run with the inoculation liquid starting containing electricity production bacterium and the phosphate buffer containing organic carbon source containing the micro- of diaphragm
Biological fuel cell makes to form the biotic environment for being easy to nitrobacteria attachment on its diaphragm material, can greatly shorten microorganism
The settling time of fuel cell nitrification.
(2) during the present invention establishes microbiological fuel cell nitrification, denitrifying bacteria also synchronous growth can be with
Synchronous nitration denitrification process occurs in reactor for realization.
(3) present invention is easy to operate using cheap diaphragm as the attachment site of nitrobacteria, and treatment effect is stablized,
And be conducive to microbiological fuel cell and obtain electricity generation performance steady in a long-term, it is very beneficial for practical application.
Detailed description of the invention
Fig. 1 is the microbiological fuel cell structural schematic diagram that embodiment 1-4 is used.
Fig. 2 is the microbiological fuel cell structural schematic diagram that embodiment 5-12 is used.
Fig. 3 is the microbiological fuel cell structural schematic diagram that embodiment 10 uses.
Fig. 4 is the microbiological fuel cell nitrification start up curve of embodiment 1.
Appended drawing reference in figure: 1- air cathode；2- diaphragm material；3- carbon brush anode；4- collector；The outer connecting resistance of 5-；6-
Feed liquor/liquid outlet；7- proton exchange membrane.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, its object is to preferably explain the present invention,
Non- limitation of the present invention.
Specific embodiment 1
Microbiological fuel cell in the present embodiment is by air cathode 1, diaphragm material 2, carbon brush anode 3,4 and of collector
Feed liquor/liquid outlet 6 forms.Reactor cavity is long 20cm, and the rectangle of high 10cm, real solution volume is 340mL, by PVC
Material is made.When assembling, 9 diameters are 2cm, and length is that the carbon brush anode 3 of 10cm is equidistantly placed in the middle position of cavity,
2 a length of 20cm, width are that the air cathode 1 of 10cm is symmetrically placed in cavity both ends, and it is thick that a piece of 0.15cm is placed on the inside of air cathode 1
Glass mat diaphragm 2, organize air cathode single-chamber microbial fuel cell in pairs, it is 100 Ω that external circuit, which connects resistance 5,.
After the completion of battery assembly, in 30 DEG C of experimental situation, the MFC that is run steadily in the long term using laboratory (such as it is empty
Gas single chamber cathode microbial fuel cell) liquid is poured out as inoculation liquid starting microbiological fuel cell, it is replaced daily for reactor
New inoculation liquid runs the 10mM phosphate buffer of the sodium acetate containing 1g/L, battery each periodic duty time after starting successfully
For for 24 hours.MFC obtains stable electricity generation performance after stable, and the biotic environment of glass mat membrane surface obtains at this time
Improve, but fiberglass surfacing does not form nitrobacteria biomembrane, the ammonia nitrogen concentration that MFC pours out liquid is not substantially reduced.With
Afterwards, with containing nitrobacteria slaughterhouse wastewater treatment factory primary sedimentation tank water outlet inoculation MFC nitrification, battery per for 24 hours for the period more
Change operation solution operation.It is lower than 5mg/L when microbiological fuel cell is discharged ammonia nitrogen, and water outlet of continuous two periods ammonia nitrogen keeps steady
Periodically, then it is assumed that stable nitration reaction is formd in battery.
The start up curve of microbiological fuel cell nitrification is as described in Figure 4.It can be seen that first passing through the operation of phosphoric acid salt promoter
Microbiological fuel cell is inoculated with nitrobacteria with slaughtering wastewater, and the settling time of nitrification shortened to 16 days from 36 days, ammonia
It is more than 90% that nitrogen removal efficiency, which reaches 98%, COD removal rate,.
Specific embodiment 2
Microbiological fuel cell structure, connection type, diaphragm material and membrane treatment method and implementation in the present embodiment
Example 1 is identical, except that starting and operation resistance are 56 Ω, operation solution is the 50mM of the sodium acetate containing 3g/L after starting successfully
Phosphate buffer, the inoculation source of nitrobacteria are aerobic activated sludge.Obtain the microbiological fuel cell for stablizing electricity generation performance
After operation aerobic activated sludge 18 days, water outlet ammonia nitrogen continuous two periods are lower than 5mg/L, it is believed that maturation is established in MFC
Nitrification, ammonia-nitrogen removal rate are more than that 95%, COD removal rate is more than 90%.
Specific embodiment 3
Microbiological fuel cell structure in the present embodiment, connection type, membrane treatment method are same as Example 1, institute
The difference is that diaphragm material is nylon diaphragm, starting and operation resistance are 10 Ω, and it is second containing 5g/L that solution is run after starting successfully
The 150mM phosphate buffer of sour sodium and the inoculation source of nitrobacteria are the water outlet of cultivating wastewater purification factory nitrification tank.Stablized
After the microbiological fuel cell of electricity generation performance is run breeding wastewater 16 days, water outlet ammonia nitrogen continuous two periods are lower than 5mg/L, it is believed that
Mature nitrification is established in MFC, ammonia-nitrogen removal rate is more than that 95%, COD removal rate is more than 90%.
Specific embodiment 4
Microbiological fuel cell structure in the present embodiment, connection type, membrane treatment method are same as Example 1, institute
The difference is that diaphragm material is J-cloth polymeric membrane, producing electricity microbionation liquid is the water outlet of city domestic sewage primary sedimentation tank, starting
Operation solution is the 250mM phosphate buffer of the sodium acetate containing 5g/L after success, and the inoculation source of nitrobacteria is nitrogen-containing wastewater
The water outlet for the treatment of plant's nitrification tank.After obtaining microbiological fuel cell operation breeding wastewater 20 days that stablize electricity generation performance, it is discharged ammonia nitrogen
Continuous two periods are lower than 5mg/L, it is believed that mature nitrification are established in MFC, ammonia-nitrogen removal rate is more than 95%, COD
Removal rate is more than 90%.
Specific embodiment 5
Microbiological fuel cell dimensions, connection type, membrane treatment method, nitrobacteria inoculation in the present embodiment
Method is same as Example 1, except that MFC contains only 1 air cathode, constitutes single chamber list cathode microbial fuel electricity
Pond, diaphragm material is carbon cloth, experimental temperature is 40 DEG C.The microbiological fuel cell operation that electricity generation performance is stablized in acquisition is butchered useless
For water treatment plant's primary sedimentation tank water after 17 days, water outlet ammonia nitrogen continuous two periods are lower than 5mg/L, it is believed that maturation is established in MFC
Nitrification, ammonia-nitrogen removal rate are more than that 95%, COD removal rate is more than 90%.
Specific embodiment 6
Microbiological fuel cell structure, connection type in the present embodiment, membrane treatment method, nitrobacteria inoculation method
It is same as Example 5, the difference is that diaphragm material is carbon paper, experimental temperature is 20 DEG C, nitrobacteria inoculation source is breeding wastewater
The water outlet for the treatment of plant's nitrification tank.After obtaining microbiological fuel cell operation breeding wastewater 16 days that stablize electricity generation performance, it is discharged ammonia nitrogen
Continuous two periods are lower than 5mg/L, it is believed that mature nitrification are established in MFC, ammonia-nitrogen removal rate is more than 95%, COD
Removal rate is more than 90%.
Specific embodiment 7
Microbiological fuel cell structure, connection type in the present embodiment, membrane treatment method, nitrobacteria inoculation method
Same as Example 5, the difference is that diaphragm material is carbon cloth, nitrobacteria inoculation source is that nitrogen-containing wastewater treatment plant nitrification tank goes out
Water.The microbiological fuel cell operation nitrogen-containing wastewater treatment plant nitrification tank for stablizing electricity generation performance is obtained after water outlet 16 days, water outlet
Ammonia nitrogen continuous two periods are lower than 5mg/L, it is believed that mature nitrification is established in MFC, ammonia-nitrogen removal rate is more than 95%,
COD removal rate is more than 90%.
Specific embodiment 8
Microbiological fuel cell structure, connection type in the present embodiment, membrane treatment method, nitrobacteria inoculation method
Same as Example 6, the difference is that diaphragm material is carbon felt, experimental temperature is 15 DEG C, and electricity production microbionation liquid is that anaerobism is active
Sludge.The microbiological fuel cell operation slaughterhouse wastewater treatment factory primary sedimentation tank water for stablizing electricity generation performance is obtained after 16 days, is discharged ammonia
Nitrogen continuous two periods are lower than 5mg/L, it is believed that mature nitrification is established in MFC, ammonia-nitrogen removal rate is more than 95%,
COD removal rate is more than 90%.
Specific embodiment 9
Microbiological fuel cell structure, connection type in the present embodiment, membrane treatment method, nitrobacteria inoculation method
It is same as Example 5, the difference is that outer connecting resistance is 300 Ω, experimental temperature is that 30 DEG C of electricity production microbionation liquid are anaerobism activity
Sludge.The microbiological fuel cell operation slaughterhouse wastewater treatment factory primary sedimentation tank water for stablizing electricity generation performance is obtained after 16 days, is discharged ammonia
Nitrogen continuous two periods are lower than 5mg/L, it is believed that mature nitrification is established in MFC, ammonia-nitrogen removal rate is more than 95%,
COD removal rate is more than 90%.
Specific embodiment 10
Microbiological fuel cell dimensions, connection type, membrane treatment method, nitrobacteria inoculation in the present embodiment
Method is same as Example 1, the difference is that placing one piece of size identical as cathode between anode and glass mat diaphragm
Cation-exchange membrane constitutes double-chamber microbiological fuel cell.Outside 30 DEG C of test temperatures and 500 Ω under connecting resistance, anode chamber
Inoculation liquid is anaerobic activated sludge, and the improvement biocompatibility inoculation liquid of cathode chamber is aerobic activated sludge.It obtains and stablizes electricity production
After 17 days, water outlet ammonia nitrogen continuous two periods are lower than the microbiological fuel cell operation slaughterhouse wastewater treatment factory primary sedimentation tank water of performance
5mg/L, it is believed that mature nitrification is established in MFC, ammonia-nitrogen removal rate is more than that 95%, COD removal rate is more than 90%.
Specific embodiment 11
Microbiological fuel cell structural schematic diagram in the present embodiment, connection type, membrane treatment method, nitrobacteria connect
Kind method is same as Example 5, except that: reactor cavity is long 4cm, the cylindrical body of diameter 3cm, real solution volume
It for 28mL, is made of pmma material, when assembling, 1 diameter is 2.5cm, and length is that the carbon brush anode 3 of 2cm is placed in chamber
The middle position of body, the glass fibre cloth diaphragm 2 that the air cathode 1 and a piece of thickness that 1 diameter is 3cm are 0.1cm are placed in cavity
One end, form single chamber list air cathode microbial fuel cell, external circuit connect resistance 5 be 1000 Ω.It obtains to stablize and produce
For the microbiological fuel cell operation slaughterhouse wastewater treatment factory primary sedimentation tank water of electrical property after 21 days, water outlet ammonia nitrogen continuous two periods are low
In 5mg/L, it is believed that establish mature nitrification in MFC, ammonia-nitrogen removal rate is more than that 95%, COD removal rate is more than 90%.
Specific embodiment 12
Microbiological fuel cell structure, connection type in the present embodiment, membrane treatment method, nitrobacteria inoculation method
It is identical as embodiment 11, except that: diaphragm material is J-cloth macromolecule cloth, test temperature is 40 DEG C, nitrobacteria
Inoculation source is the water outlet of cultivating wastewater purification factory nitrification tank.Obtain the microbiological fuel cell operation breeding wastewater for stablizing electricity generation performance
After treatment plant's nitrification tank is discharged 19 days, water outlet ammonia nitrogen continuous two periods are lower than 5mg/L, it is believed that maturation is established in MFC
Nitrification, ammonia-nitrogen removal rate are more than that 95%, COD removal rate is more than 90%.
Claims (5)
1. the method for quickly establishing microbiological fuel cell nitrification, which comprises the following steps:
(1) diaphragm material is handled, diaphragm material surface is made to form the biotic environment that nitrobacteria is easy to grow film forming；Tool
Body step are as follows:
Diaphragm material is cut into size identical with cathode, is placed on the inside of air cathode Catalytic Layer, forms and has with carbon brush anode
There is the microbiological fuel cell of diaphragm single chamber, is subsequently filled electricity production microbionation liquid；15~40 DEG C at a temperature of, use is external
Electric-resistivity method starts the microbiological fuel cell；After starting successfully, which is run with the phosphate buffer containing organic carbon source
Object fuel cell, it is every to be for 24 hours a cycle of operation and replace inoculation liquid, until microbiological fuel cell obtains stable electricity production
Can, the biotic environment for being easy to nitrobacteria growth film forming is formed on diaphragm material at this time；
The electricity production microbionation liquid is any one following: long-term running air single chamber cathode microbial fuel cell
Water outlet, city domestic sewage treatment plant primary sedimentation tank water, anaerobic activated sludge or aerobic activated sludge；It is described containing organic carbon source
Phosphate buffer refers to: using sodium acetate as the phosphate buffer of organic carbon source；According to the difference of service condition, acetic acid is set
Na concn is 1~5g/L, and phosphate buffer is 10~250mM；
(2) solution containing nitrobacteria is run with microbiological fuel cell obtained by step (1), for realizing connecing for nitrobacteria
Kind, nitrobacteria biomembrane is formed on diaphragm material；Specific steps are as follows:
Use external several periods of electric-resistivity method continuous operation to establish MFC nitrification, per being for 24 hours cycle of operation and more
Change the solution containing nitrobacteria；When the ammonia-nitrogen content of microbiological fuel cell water outlet is lower than 5mg/L, and continuous two periods go out
When water ammonia nitrogen keeps stablizing, then it is assumed that form stable nitration reaction in battery, it is thin to have formed nitrification on diaphragm material at this time
Bacterium biomembrane, microbiological fuel cell nitrification have been established.
2. the method according to claim 1, wherein the diaphragm material is any one following: glass
Fibrofelt, glass fabric, nylon diaphragm, carbon cloth, carbon paper, carbon felt or macromolecule cloth.
3. the method according to claim 1, wherein the microbiological fuel cell structure is following any one
Kind: double air cathode single-chamber microbial fuel cells, single air cathode single-chamber microbial fuel cell or air cathode dual chamber are micro-
Biological fuel cell.
4. the method according to claim 1, wherein being established in microbiological fuel cell starting and nitrification
Outer connecting resistance used in journey, resistance value are 7000-20000 Ω/cm according to the area setting resistance of air cathode2Cathode plane
Product.
5. the method according to claim 1, wherein the solution containing nitrobacteria is following any one
Kind: at slaughterhouse wastewater treatment factory primary sedimentation tank water, aerobic activated sludge, the water outlet of cultivating wastewater purification factory nitrification tank or nitrogen-containing wastewater
Manage the water outlet of factory's nitrification tank.
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| CN107338193A (en) * | 2017-06-27 | 2017-11-10 | 浙江大学 | A kind of enrichment method of microorganism for biological eliminating high concentration nitrogen-containing compound |
| CN107349781B (en) * | 2017-06-27 | 2019-11-08 | 浙江大学 | A kind of efficient removal ultrahigh concentration NOxMicroorganism orientation acclimation method |
| CN109148923B (en) * | 2018-08-14 | 2021-07-09 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for accelerating power generation starting of microbial fuel cell |
| CN109841883A (en) * | 2019-02-25 | 2019-06-04 | 电子科技大学中山学院 | Use method of single-chamber air cathode MFC |
| CN114524493B (en) * | 2022-01-25 | 2023-04-07 | 中南大学 | Ammonia recovery device and method for electrochemically treating nitrate wastewater |
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| CN103427102A (en) * | 2013-08-30 | 2013-12-04 | 华南理工大学 | Phycomycetes microbial fuel cell and preparation method and application of phycomycetes microbial fuel cell |
| CN104377378A (en) * | 2014-04-28 | 2015-02-25 | 华北电力大学 | Microbial electrochemical apparatus and method for restoring nitrate-polluted underground water |
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