CN107338193A - A kind of enrichment method of microorganism for biological eliminating high concentration nitrogen-containing compound - Google Patents
A kind of enrichment method of microorganism for biological eliminating high concentration nitrogen-containing compound Download PDFInfo
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- CN107338193A CN107338193A CN201710497512.7A CN201710497512A CN107338193A CN 107338193 A CN107338193 A CN 107338193A CN 201710497512 A CN201710497512 A CN 201710497512A CN 107338193 A CN107338193 A CN 107338193A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to biological denitrificaion field, it is desirable to provide a kind of enrichment method of microorganism for biological eliminating high concentration nitrogen-containing compound.It is to mix the anaerobic sludge of a variety of actual waste water treatment plants, inoculation source is obtained after agitated, filtering and centrifugal treating；Then chemical reagent is added, is run after being inoculated with microbiological fuel cell, is finally enriched with the microorganism for biological eliminating high concentration nitrogen-containing compound in a fuel cell.The present invention is used as carrier by the use of microbiological fuel cell, pass through bioelectrochemistry mode, with reference to specific culture environment, enable nitrification, denitrifying microorganism to stablize to grow and largely breed, and adsorb on battery electrode, form fine and close membrane structure, reach can efficient removal high concentration nitrogen-containing compound effect.Present invention introduces a variety of actual waste waters, enrich microbial population, improve the stability of system；Construction is advantageous to nitrification, the environment of denitrifying microorganism growth and breeding, selection index system of being all risen to microbial species, reaches the purpose of enrichment.
Description
Technical field
The invention belongs to biological denitrificaion field, is related to a kind of microorganism for biological eliminating high concentration nitrogen-containing compound
Enrichment method.
Background technology
Organic matter, nitrogen substance, phosphorus containg substances are common water pollutants.Implemented " a control pair since 2000 in China
It is up to standard " after, the Organic Pollution in waste water has obtained effective containment, and nitrate pollution rises to the main environment faced for China and asked
Topic.The discharge capacity of current national nitrogen pollutant has also surpassed far beyond the environmental carrying capacity of receiving water body, nitrogen pollutant
Crossing COD turns into the primary index for influenceing quality of water environment.Nitrate pollution has jeopardized all conglomeraties such as agricultural, fishery, tourist industry, and
Drinking water hygiene and food security are constituted a serious threat.The control of nitrate pollution turns into currently urgently to be resolved hurrily one with removing
Great environmental protection subject.Therefore, China newly includes nitrate pollution " 13 " pollutant emission factor system, as master
One of pollutant is wanted, and overall control is implemented to it.
The handling process of nitrogenous effluent mainly includes Physical, chemical method, ion-exchange, artificial swamp method and biology
Method.Wherein, Physical, chemical method, ion-exchange and artificial swamp method or because cost is too high, or because of poor processing effect, or
Because of narrow application range, large-scale application is not obtained.It is denitrogenation and bioanalysis has the advantages that cost is low, effect is good, pollution-free
Main stream approach.
Bio-denitrification technology mainly includes nitrification, denitrification.Nitrification refers to that heterotrophic microorganism will under aerobic condition
Ammonia catalytic is the process of nitrite and nitrate；Denitrification refers to that microorganism is by nitrite under anoxic conditions
The process of nitrogen is reduced to the catalysis of nitrate salt.
In soil, water in nature, naturally occurring several microorganisms with nitrification and denitrification performance, but because
Concentration is too low, it is impossible to plays effective purification soil and the effect of the purification of the nitrogen substance in water.It is so good in order to obtain
Nitrification and denitrification performance, it is necessary to be enriched with to nitrification and denitrification microorganism, improve its abundance.
The content of the invention
The technical problem to be solved in the present invention is to overcome deficiency of the prior art, there is provided it is high that one kind is used for biological eliminating
The enrichment method of the microorganism of concentration nitrogen-containing compound.
To solve technical problem, solution of the invention is as follows：
A kind of enrichment method of the microorganism for biological eliminating high concentration nitrogen-containing compound is provided, comprised the following steps：
Step 1：The processing of inoculation source
Pig slaughtering field waste water treatment plant, food processing wastewater treatment plant, Refuse Leachate Treatment Plant, city will be derived from
The anaerobic sludge of sewage treatment plants is mixed at 1: 1: 1: 1 by volume, and supernatant is taken after agitated, filtering and centrifugal treating,
Using positioned at the liquid of the volume of supernatant lower floor 1/10 as inoculation source；
Step 2：Add chemical reagent
Inoculation trace element solution is added by 10mL/L dosage into inoculation source, inoculation liquid is obtained after mixing；
In the inoculation trace element solution, include each composition of following mass concentrations：11.3g/L Na2HPO4·
12H2O、3.1g/L NaH2PO4·12H2O、0.1g/L NH4Cl、0.1g/L KCl、0.1g/L NaCl、0.005g/L
CaCl、0.002g/L MgSO4、0.003g/L MnSO4、0.001g/L FeSO4、0.001g/L CoSO4、0.001g/
LZnSO4、0.001g/L CuSO4；
Step 3：Inoculation
Inoculation liquid is added in the microbiological fuel cell that assembles, fuel cell operation in open circuit or it is closed circuit under the conditions of,
Environment temperature is 20~40 DEG C, and fresh inoculation liquid is changed every 24h；
Daily water outlet to fuel cell is sampled, and measures its ammonium ion, nitrate ion, nitrite ion
Concentration；When difference is less than 5% to the concentration of ammonium ion in water outlet, nitrate ion, nitrite ion within continuous three days, table
Show to be inoculated with fuel cell and complete；
Step 4：Enrichment
By same ratio relation in step 2, add trace element solution into deionized water, and ammonium addition, nitrate nitrogen,
Nitrite nitrogen and sodium acetate, obtain running liquid after mixing；Run equal with the final concentration of the ammonia nitrogen of nitrogen metering, nitrate nitrogen, nitrite nitrogen in liquid
For 1000mg/L, the final concentration of 8g/L of sodium acetate；
Inoculation liquid in fuel cell is all replaced with operation liquid, and fresh operation liquid is changed every 24h；Continuous fortune
After row 3 months, the microorganism for biological eliminating high concentration nitrogen-containing compound is finally enriched in a fuel cell.
In the present invention, described microbiological fuel cell is the microbiological fuel cell of single cell structure.
Inventive principle describes：
Inoculation source used in the present invention is mixed by the anaerobic sludge of a variety of actual waste water treatment plants, including but not limited to
Slaughterhouse water treatment plant, waste water treatment plant of food processing factory, Refuse Leachate Treatment Plant, city domestic sewage treatment plant are detested
Oxygen sludge.The microorganism wherein to play a role is mainly a variety of denitrifying bacteriums.
The present invention is by the use of microbiological fuel cell as carrier, by bioelectrochemistry mode, with reference to specific culture environment,
Enable nitrification, denitrifying microorganism to stablize to grow and largely breed, and adsorb on battery electrode, form the film knot of densification
Structure, reach can efficient removal high concentration nitrogen-containing compound effect.
Compared with prior art, beneficial effects of the present invention are as follows：
1st, a variety of actual waste waters are introduced, enrich microbial population, improve the stability of system；
2nd, inoculation source is carried out the pretreatment such as centrifuging, improves bacteria abundance；
3rd, using bioelectrochemistry system culture, the activity of microorganism is added；
4th, by adding chemical reagent, construction is advantageous to nitrification, the environment of denitrifying microorganism growth and breeding, to microorganism
Selection index system of all rising is planted, reaches the purpose of enrichment；
5th, the microorganism after taming can be with the nitrogen substance of efficient process high concentration, including ammonia nitrogen, nitrate nitrogen and nitrite nitrogen；Can
The concentration range of processing is respectively：30~1000mg/L of ammonia nitrogen, 30~1000mg/L of nitrate nitrogen, 30~1000mg/L of nitrite nitrogen.
Brief description of the drawings
Fig. 1 is the process flow diagram of this technology invention.
Fig. 2 is microbiological fuel cell structural representation in the present invention.
Reference in figure：1 anode；2 negative electrodes；3 single chamber MFC reactors；4 loads；5 switches.
Embodiment
Describe the embodiment of the present invention in detail with reference to technical scheme and accompanying drawing, but the present invention not only limits to
In following example.
From pig slaughtering field waste water treatment plant, food processing wastewater treatment plant, Refuse Leachate Treatment Plant, city
In the anaerobic sludge of sewage treatment plants, containing certain density various nitrification/denitrifying microorganisms.
The preparation method of inoculation source：By pig slaughtering field waste water treatment plant, waste water treatment plant of food processing factory, garbage filter
Liquid treatment plant, the anaerobic sludge of city domestic sewage treatment plant mix at 1: 1: 1: 1 by volume, at agitated, filtering and centrifugation
Supernatant is taken after reason, using positioned at the liquid of the volume of supernatant lower floor 1/10 as inoculation source.
Applicant promises to undertake：The inoculation source is provided to the public out of from the patent application 20 years, for realizing, utilizing
Technical scheme of the present invention.
Culture and domestication place of the microbiological fuel cell of the invention by the use of single chamber as reactor.Single chamber MFC reacts
The concrete structure of device 3 is as shown in Figure 2.Single chamber MFC reactors 3 are made up of negative electrode 2, anode 1 and external circuit, and external circuit includes load
4 and switch 5.
The enrichment method of microorganism of the present invention for biological eliminating high concentration nitrogen-containing compound, including following step
Suddenly：
Step 1：The processing of inoculation source
Pig slaughtering field waste water treatment plant, food processing wastewater treatment plant, Refuse Leachate Treatment Plant, city will be derived from
The anaerobic sludge of sewage treatment plants is mixed at 1: 1: 1: 1 by volume, and supernatant is taken after agitated, filtering and centrifugal treating,
Using positioned at the liquid of the volume of supernatant lower floor 1/10 as inoculation source；
Step 2：Add chemical reagent
Inoculation trace element solution is added by 10mL/L dosage into inoculation source, inoculation liquid is obtained after mixing；
In the inoculation trace element solution, include each composition of following mass concentrations：11.3g/L Na2HPO4·
12H2O、3.1g/L NaH2PO4·12H2O、0.1g/L NH4Cl、0.1g/L KCl、0.1g/L NaCl、0.005g/L
CaCl、0.002g/L MgSO4、0.003g/L MnSO4、0.001g/L FeSO4、0.001g/L CoSO4、0.001g/
LZnSO4、0.001g/L CuSO4；
Step 3：Inoculation
Inoculation liquid is added in the microbiological fuel cell that assembles, fuel cell operation in open circuit or it is closed circuit under the conditions of,
Environment temperature is 20~40 DEG C, and fresh inoculation liquid is changed every 24h；
Daily water outlet to fuel cell is sampled, and measures its ammonium ion, nitrate ion, nitrite ion
Concentration；When difference is less than 5% to the concentration of ammonium ion in water outlet, nitrate ion, nitrite ion within continuous three days, table
Show to be inoculated with fuel cell and complete；
Step 4：Enrichment
By same ratio relation in step 2, add trace element solution into deionized water, and ammonium addition, nitrate nitrogen,
Nitrite nitrogen and sodium acetate, obtain running liquid after mixing；Run equal with the final concentration of the ammonia nitrogen of nitrogen metering, nitrate nitrogen, nitrite nitrogen in liquid
For 1000mg/L, the final concentration of 8g/L of sodium acetate；
Inoculation liquid in fuel cell is all replaced with operation liquid, and fresh operation liquid is changed every 24h；Continuous fortune
After row 3 months, the microorganism for biological eliminating high concentration nitrogen-containing compound is finally enriched in a fuel cell.
Remove the operating instruction of high concentration nitrogen-containing compound：
Appropriate sodium acetate is added in the deionized water solution added with 10mL/L trace elements, then adds difference
Ammonium chloride, potassium nitrate, the natrium nitrosum of concentration, stir, and are then added to of the present invention by being enriched with and cultivating
In the good microbiological fuel cell for possessing Capability of Nitrification/denitrification In A, microbiological fuel cell is placed in open circuit or closed circuit condition
Lower operation.Solution example was taken to carry out ammonia nitrogen, nitrate nitrogen, nitrite nitrogen concentration determination from reactor at interval of 1 hour.
Claims (2)
1. the enrichment method of a kind of microorganism for biological eliminating high concentration nitrogen-containing compound, it is characterised in that including following
Step：
Step 1：The processing of inoculation source
Pig slaughtering field waste water treatment plant, food processing wastewater treatment plant, Refuse Leachate Treatment Plant, urban life will be derived from
The anaerobic sludge of sewage treatment plant mixes at 1: 1: 1: 1 by volume, supernatant is taken after agitated, filtering and centrifugal treating, by position
In the volume of supernatant lower floor 1/10 liquid as inoculation source；
Step 2：Add chemical reagent
Inoculation trace element solution is added by 10mL/L dosage into inoculation source, inoculation liquid is obtained after mixing；
In the inoculation trace element solution, include each composition of following mass concentrations：11.3g/L Na2HPO4·12H2O、
3.1g/L NaH2PO4·12H2O、0.1g/L NH4Cl、0.1g/L KCl、0.1g/L NaCl、0.005g/L CaCl、
0.002g/L MgSO4、0.003g/L MnSO4、0.001g/L FeSO4、0.001g/L CoSO4、0.001g/LZnSO4、
0.001g/L CuSO4；
Step 3：Inoculation
Inoculation liquid is added in the microbiological fuel cell assembled, fuel cell operation is under the conditions of opening a way or be closed circuit, environment
Temperature is 20~40 DEG C, and fresh inoculation liquid is changed every 24h；
Daily water outlet to fuel cell is sampled, measure its ammonium ion, nitrate ion, nitrite ion it is dense
Degree；When difference is less than 5% to the concentration of ammonium ion in water outlet, nitrate ion, nitrite ion within continuous three days, combustion is represented
Expect to be inoculated with battery and complete；
Step 4：Enrichment
By same ratio relation in step 2, trace element solution, and ammonium addition, nitrate nitrogen, nitrous are added into deionized water
Nitrogen and sodium acetate, obtain running liquid after mixing；It is with the final concentration of the ammonia nitrogen of nitrogen metering, nitrate nitrogen, nitrite nitrogen in operation liquid
1000mg/L, the final concentration of 8g/L of sodium acetate；
Inoculation liquid in fuel cell is all replaced with operation liquid, and fresh operation liquid is changed every 24h；Continuous operation 3
After individual month, the microorganism for biological eliminating high concentration nitrogen-containing compound is finally enriched in a fuel cell.
2. method according to claim 1, it is characterised in that the microbiological fuel cell is single chamber air cathode structure
Microbiological fuel cell.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113186146A (en) * | 2021-05-13 | 2021-07-30 | 深高蓝德环保科技集团股份有限公司 | Method for culturing microbial agent by using town landfill leachate |
| CN113484398A (en) * | 2021-06-11 | 2021-10-08 | 浙江大学 | Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand |
| CN113615627A (en) * | 2021-08-26 | 2021-11-09 | 大连海洋大学 | Biological-electrochemical culture seawater purification system for enriching nitrifying microorganisms |
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| CN106450398A (en) * | 2016-10-06 | 2017-02-22 | 浙江大学 | Method for quickly building microbial fuel cell nitrification system |
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- 2017-06-27 CN CN201710497512.7A patent/CN107338193A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106450398A (en) * | 2016-10-06 | 2017-02-22 | 浙江大学 | Method for quickly building microbial fuel cell nitrification system |
Non-Patent Citations (2)
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| DALMACIJA B等: "Purification of high-salinity wastewater by activated sludge process", 《WATER RESEARCH》 * |
| 黄浩斌等: "单室空气阴极微生物燃料电池硝酸根去除系统的建立和性能研究", 《环境科学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113186146A (en) * | 2021-05-13 | 2021-07-30 | 深高蓝德环保科技集团股份有限公司 | Method for culturing microbial agent by using town landfill leachate |
| CN113484398A (en) * | 2021-06-11 | 2021-10-08 | 浙江大学 | Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand |
| CN113484398B (en) * | 2021-06-11 | 2022-05-27 | 浙江大学 | Bioelectrochemical method for synchronously detecting nitrite and biochemical oxygen demand |
| CN113615627A (en) * | 2021-08-26 | 2021-11-09 | 大连海洋大学 | Biological-electrochemical culture seawater purification system for enriching nitrifying microorganisms |
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