CN103949116A - Compounded gel filter cotton capable of being used as microbial carrier and preparation method of filter cotton - Google Patents
Compounded gel filter cotton capable of being used as microbial carrier and preparation method of filter cotton Download PDFInfo
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- CN103949116A CN103949116A CN201410170007.8A CN201410170007A CN103949116A CN 103949116 A CN103949116 A CN 103949116A CN 201410170007 A CN201410170007 A CN 201410170007A CN 103949116 A CN103949116 A CN 103949116A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention belongs to the technical field of filter cotton, and particularly relates to compounded gel filter cotton capable of being used as a microbial carrier. The filter cotton comprises porous cotton and hydrogel, wherein the porous cotton and the hydrogel form an interlaced netting structure; the volume of the hydrogel is 5%-50% of that of gaps of the porous cotton; the pore size of small pores in the porous cotton is 1 micron to 1 centimeter. The compounded gel filter cotton disclosed by the invention is formed by organically combining two materials including the porous cotton and gel in a crosslinking manner based on layer-layer self-adsorption, a gel net is arranged among the gaps of sponges in a penetrating manner to increase the bacterium carrying capacity, while the porous cotton is used for providing a mechanical support and an extra passage for conveying oxygen and nutrients to a gel layer; in addition, the pore size of the gel layer is smaller than those of the small pores in the porous cotton, so that the filtration effect is enhanced as well.
Description
Technical field
The invention belongs to and filter continuous technical field, relate in particular to a kind of can be as gel combined filtration silk floss and the preparation method of microbe carrier.
Background technology
Immobilized microorganism technique is an emerging technology in modern biological project field, and it is in the area of space that free cell, microorganism or enzyme are positioned to limit of the means by chemistry or physics, makes it keep method active and that can recycle.Microorganism is mainly employed with the form of dissociating before this.But free microorganism is easily washed away by current under flox condition, under hydrostatic condition, easily by other biological, eaten, and it is difficult to stably bring into play for a long time function while lacking the carrier of absorption and growth and breeding.Therefore people start to consider to utilize immobilized microorganism technique to replace traditional method of shedding free bacteria in water treatment field.The former has obvious superiority compared with the latter, is mainly manifested in: treatment effeciency is high, stability is strong, can purifying and keep that high-efficiency strain, microorganism concn are high, good effect of separating solid from liquid.
Immobilization method of microorganism, mainly contains the several methods such as adsorption (combination) technique for fixing, crosslinked (binding) immobilization, embedding (polymer) immobilization and self immobilization (self-crosslinking immobilization between microbial cell).In various microorganism immobilization methods, investment is low with its density, be easy to fluidised superiority becomes the most widely used method of current research.The carrier material of conventional investment fixation of microbe, has agar, sodium alginate, carragheen and synthetic macromolecular compound polyacrylamide (PACAM), the polyvinyl alcohol (PVA) of bio-based high molecular polysaccharide.Wherein, bio-based high-molecular gel is if sodium alginate is because the process of its gel forming is simple, and mild condition, does not have toxicity to microbial cell, so it is the most extensive in the application of the embedding of microbial cell.But investment complicated process of preparation, preparation cost is high, material mechanical performance is poor, resistance to mass tranfer is large, larger to the metabolic effect of microbial growth and product.And at present conventionally on the market take the tender sponge of biochemistry that polyurethane, polyethers is raw material and except filtration, also all have the effect of absorption fixation of microbe concurrently, but the capacity of year bacterium is very little.
Summary of the invention
The object of the invention is to provide for the deficiencies in the prior art a kind of can be continuous as the gel combined filtration of microbe carrier.
For achieving the above object, the present invention adopts following technical scheme: a kind of can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, the tender hydrogel of porous forms interspersed net line structure mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5%～50%, and the small aperture in porous silk floss is 1 μ m～1cm.
Porous silk floss is that the biochemical silk floss of polyurethanes sponge, polyethers or polyesters are biochemical continuous.
The beneficial effect of gel combined filtration silk floss of the present invention, gel combined filtration silk floss of the present invention by the tender gel bi-material of porous by being organically combined into based on the mode from cross-linking layer by layer, gel network is interted has increased a year bacterium capacity between the space of sponge, and porous silk floss provides the additional channels of mechanics support and delivering oxygen, nutrient for gel layer, because the aperture of gel layer is little compared with the small aperture in porous silk floss, also improve state's filter effect simultaneously.
The present invention also provide a kind of can be as the preparation method of the gel combined filtration silk floss of microbe carrier, its preparation process comprises:
The first step, immerses plastic Polymer Solution 1～10 minute by porous silk floss;
Second step, puts into the porous silk floss soaking through the first step to solidify solution or under the cryogenic conditions of-20 ℃～0 ℃, form gel combined filtration silk floss;
The 3rd step, the continuous microbial solution that immerses of the gel combined filtration that second step is made, standing adsorption 0.5～5 hour, makes finished product.
Plastic Polymer Solution is that sodium alginate, agar-agar and polyvinyl alcohol mix the composite solution forming, and wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5%～20%.
Solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
Microbial solution includes aerobic microbiological, and aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the mass fraction that aerobic microbiological accounts in microbial solution is 1%～30%.
The present invention also provides the another kind can be as the preparation method of the gel combined filtration silk floss of microbe carrier, and its preparation process comprises:
The first step, is mixed to form microbiological gum liquid solution by plastic Polymer Solution and microbial solution equal-volume;
Second step, the microbiological gum liquid solution that the continuous immersion of the porous first step is made 1～10 minute;
The 3rd step, puts into the porous silk floss of processing through second step to solidify solution or make finished product under the cryogenic conditions of-20 ℃～0 ℃.
Also comprise the 4th step, with clear water, clean the porous silk floss of processing through the 3rd step, wash away the solution of ungelled part, then continuous immersion of porous solidified to solution 1～10 minute, solidify solution and put into together-20 ℃～0 ℃ low temperature environment porous is tender again, make to solidify solution gel.
Plastic Polymer Solution is that sodium alginate, agar-agar and polyvinyl alcohol mix the composite solution forming, and wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5%～20%.
Solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
Microbial solution includes aerobic microbiological, and aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the mass fraction that aerobic microbiological accounts in microbial solution is 1%～30%.
Preparation method's of the present invention beneficial effect: preparation method of the present invention is simple to operate, the gel combined filtration silk floss making has increased a year bacterium capacity, and porous silk floss provides the additional channels of mechanics support and delivering oxygen, nutrient for gel layer, because the aperture of gel layer is little compared with the small aperture in porous silk floss, also improve state's filter effect simultaneously.
The specific embodiment
A kind of can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, the tender hydrogel of porous forms interspersed net line structure mutually, and the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5%～50%, and the small aperture in porous silk floss is 1 μ m～1cm.
Porous silk floss is that the biochemical silk floss of polyurethanes sponge, polyethers or polyesters are biochemical continuous.
Embodiment 1
The preparation of agar-agar gel composite filter silk floss: take polyurethane sponge as base material (buying on market), its voidage is 60%, and average pore size is 2mm, and thickness is 8cm.Preparation process:
The first step, immerses the sponge of about polyurethane sponge bottom 3cm in 10% the agar-agar solution of 90 ℃ standing 3 minutes;
Second step is put into the polyurethane sponge of the first step refrigerator of 0 ℃ of degree together with agar-agar solution, places 2 hours cooled and solidified plastics;
The 3rd step, puts into the bacterium original solution of the photosynthetic bacteria that contains 20% mass fraction by the polyurethane sponge of processing through second step, places and within 1 hour, allows microbe passive adsorb, and the gel filter silk floss that is loaded with like this compound bacteria has just been made.
Embodiment 2
The preparation of polyvinyl alcohol gel composite filter silk floss: think that polyurethane sponge is base material (buying on market), its voidage is 70%, and average pore size is 2mm, and thickness is 8cm.Preparation process:
The first step, immerses the sponge of about polyurethane sponge bottom 3cm in 15% polyvinyl alcohol (trade mark 1799) solution of 90 ℃ standing 3 minutes;
Second step is put into the polyurethane sponge of the first step refrigerator of-20 ℃ together with poly-vinyl alcohol solution, places 10 hours cooled and solidified plastics;
The 3rd step, puts into the bacterium original solution of the photosynthetic bacteria that contains 20% mass fraction by the polyurethane sponge of processing through second step, places and within 1 hour, allows microbe passive adsorb, and the gel filter silk floss that is loaded with like this polyvinyl alcohol of compound bacteria has just been made.
Embodiment 3
The preparation method of calcium alginate gel combined filtration silk floss: the biochemical silk floss of the polyesters of take is base material (buying on market), and its voidage is 75%, and average pore size is 1mm, and thickness is 5cm.Preparation process:
The first step, is mixed into microbiological gum liquid solution by the solution equal-volume of the former bacterium of nitrobacteria that contains 4% sodium alginate gelling solution and 10%;
Second step, in the microbiological gum liquid solution that the sponge immersion first step of the biochemical continuous bottom 2cm of polyesters left and right is made, stops 5 minutes;
The 3rd step, by biochemical continuous 2% the calcium chloride solution of putting into again of the polyesters of processing through second step, within standing 3 minutes, solidify plastic, after plastic, with clear water, clean gel surface, and then repeat twice of the above-mentioned first step to the three step plastics operation, make gel layer thickness reach pre-provisioning request, carry so germy gel combined filtration silk floss and just made.
Embodiment 4
The preparation of calcium alginate-polyvinyl alcohol gel composite filter silk floss: the biochemical silk floss of the polyesters of take is base material (buying on market), and its voidage is 75%, and average pore size is 2mm, and thickness is 10cm.Preparation process:
The first step, is mixed into microbiological gum liquid solution by containing 5% sodium alginate gelling solution and 10% nitrobacteria solution equal-volume;
Second step, the microbiological gum liquid solution this first step being made with syringe injects the biochemical silk floss of polyesters, makes microbiological gum liquid solution be evenly distributed on 2cm place, the biochemical continuous bottom of polyesters, and to make microbiological gum liquid solution form thickness be 1cm contains glue thin layer;
The 3rd step, puts into 2% calcium gluconate solution by the biochemical silk floss of the polyesters of processing through second step, within standing 5 minutes, solidifies plastic;
The 4th step, with clear water, clean the biochemical silk floss of the polyesters of processing through the 3rd step, wash away the solution of ungelled part, then the sponge at 4cm place, the biochemical continuous bottom of polyesters is all immersed in 8% polyvinyl alcohol (trade mark 1799) solution of 40 ℃, stop 10 minutes, finally put into-20 ℃ of refrigerators 2 hours, make poly-vinyl alcohol solution gel, the gel filter silk floss that is loaded with like this calcium alginate-polyvinyl alcohol of compound bacteria has just been made.
The announcement of book and instruction according to the above description, those skilled in the art in the invention can also change and revise above-mentioned embodiment.Therefore, the present invention is not limited to the above-mentioned specific embodiment, and any apparent improvement, replacement or modification that every those skilled in the art have done on basis of the present invention all belong to protection scope of the present invention.In addition,, although used some specific terms in this description, these terms just for convenience of description, do not form any restriction to the present invention.
Claims (10)
- One kind can be continuous as the gel combined filtration of microbe carrier, comprise the tender hydrogel of porous, it is characterized in that: the tender hydrogel of porous forms interspersed net line structure mutually, the volume parts that hydrogel accounts for the continuous cavity volume of porous is 5%～50%, and the small aperture in porous silk floss is 1 μ m～1cm.
- 2. according to claim 1 can be continuous as the gel combined filtration of microbe carrier, it is characterized in that: porous continuous for polyurethanes sponge, the biochemical silk floss of polyethers or polyesters biochemical continuous.
- Described in claim 1 or 2 can be as the preparation method of the gel combined filtration silk floss of microbe carrier, its preparation process comprises:The first step, immerses plastic Polymer Solution 1～10 minute by porous silk floss;Second step, puts into the porous silk floss soaking through the first step to solidify solution or under the cryogenic conditions of-20 ℃～0 ℃, form gel combined filtration silk floss;The 3rd step, the continuous microbial solution that immerses of the gel combined filtration that second step is made, standing adsorption 0.5～5 hour, makes finished product.
- 4. according to claim 3 can be as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: plastic Polymer Solution is that sodium alginate, agar-agar and polyvinyl alcohol mix the composite solution forming, wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5%～20%.
- 5. according to claim 3 can, as gel combined filtration silk floss and the preparation method of microbe carrier, it is characterized in that: solidify solution and comprise water miscible calcium salt and water, wherein, the mass fraction of calcium salt is 0.1% ~ 20%.
- 6. according to claim 3 can be as gel combined filtration silk floss and the preparation method of microbe carrier, it is characterized in that: microbial solution includes aerobic microbiological, aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the mass fraction that aerobic microbiological accounts in microbial solution is 1%～30%.
- Described in claim 1 or 2 can be as the preparation method of the gel combined filtration silk floss of microbe carrier, its preparation process comprises:The first step, is mixed to form microbiological gum liquid solution by plastic Polymer Solution and microbial solution equal-volume;Second step, the microbiological gum liquid solution that the continuous immersion of the porous first step is made 1～10 minute;The 3rd step, puts into the porous silk floss of processing through second step to solidify solution or make finished product under the cryogenic conditions of-20 ℃～0 ℃.
- 8. according to claim 7 can be as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: also comprise the 4th step, with clear water, clean the porous silk floss of processing through the 3rd step, wash away the solution of ungelled part, then continuous immersion of porous solidified to solution 1～10 minute, solidify solution and put into together-20 ℃～0 ℃ low temperature environment porous is tender again, make to solidify solution gel.
- 9. according to claim 7 can be as the preparation method of the gel combined filtration silk floss of microbe carrier, it is characterized in that: plastic Polymer Solution is that sodium alginate, agar-agar and polyvinyl alcohol mix the composite solution forming, wherein, sodium alginate, agar-agar and the polyvinyl alcohol mass fraction in plastic Polymer Solution is 0.5%～20%.
- 10. according to claim 7 can be as gel combined filtration silk floss and the preparation method of microbe carrier, it is characterized in that: microbial solution includes aerobic microbiological, aerobic microbiological is nitrobacteria and/or photosynthetic bacteria, and the mass fraction that aerobic microbiological accounts in microbial solution is 1%～30%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104774830A (en) * | 2015-03-27 | 2015-07-15 | 成都百特万合医药科技有限公司 | Transaminase immobilization method |
| CN109351055A (en) * | 2018-10-24 | 2019-02-19 | 武汉大学 | A kind of hydrate gel type filter core and preparation method thereof |
| CN111995795A (en) * | 2020-08-27 | 2020-11-27 | 江南大学 | Amphiphilic polyurethane sponge and preparation method thereof |
| US20210220504A1 (en) * | 2018-06-04 | 2021-07-22 | Filkim Filtre Ve Kimya Sanayi Ticaret Anonim Sirketi | Ultra high efficiency organic gel microbial air filtration and production system |
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| US20070170114A1 (en) * | 2006-01-24 | 2007-07-26 | Michael Richter | Method and biological product for the residential and commercial aquatic environment |
| CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070170114A1 (en) * | 2006-01-24 | 2007-07-26 | Michael Richter | Method and biological product for the residential and commercial aquatic environment |
| CN101288778A (en) * | 2008-06-18 | 2008-10-22 | 天津大学 | Preparation method of porous bacteria cellulose sponges |
| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774830A (en) * | 2015-03-27 | 2015-07-15 | 成都百特万合医药科技有限公司 | Transaminase immobilization method |
| US20210220504A1 (en) * | 2018-06-04 | 2021-07-22 | Filkim Filtre Ve Kimya Sanayi Ticaret Anonim Sirketi | Ultra high efficiency organic gel microbial air filtration and production system |
| CN109351055A (en) * | 2018-10-24 | 2019-02-19 | 武汉大学 | A kind of hydrate gel type filter core and preparation method thereof |
| CN111995795A (en) * | 2020-08-27 | 2020-11-27 | 江南大学 | Amphiphilic polyurethane sponge and preparation method thereof |
| CN111995795B (en) * | 2020-08-27 | 2021-09-28 | 江南大学 | Amphiphilic polyurethane sponge and preparation method thereof |
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| CN103949116B (en) | 2016-03-09 |
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Effective date of registration: 20200113 Address after: 315500 Dongfeng Road, Fenghua District, Ningbo, Zhejiang Province, No. 80 Patentee after: Ningbo reputation Environment Technology Co., Ltd. Address before: 7, building 7006, 1558 Jiangnan Road, 315048 hi tech Zone, Zhejiang, Ningbo Patentee before: NINGBO SUNNY NOVEL MATERIAL TECHNOLOGY CO., LTD. |
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