CN111440757A - Microcarrier suitable for cell growth and method for culturing microcarrier in microenvironment - Google Patents
Microcarrier suitable for cell growth and method for culturing microcarrier in microenvironment Download PDFInfo
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- CN111440757A CN111440757A CN202010231537.4A CN202010231537A CN111440757A CN 111440757 A CN111440757 A CN 111440757A CN 202010231537 A CN202010231537 A CN 202010231537A CN 111440757 A CN111440757 A CN 111440757A
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Abstract
The invention discloses a microcarrier suitable for cell growth and a method for culturing the microcarrier in a microenvironment, which comprises a plurality of cells; a carrying solution for carrying the plurality of cells and mixing to form a plurality of inner cores; one is an outer shell layer of alginic acid, and covers the periphery of each inner core, and then the alginic acid and calcium chloride solution are mixed and mildly crosslinked to form a plurality of porous microcarriers, and a carrying solution with the function of enhancing cell adhesion is arranged in a culture medium in the outer shell layer, and the outer shell layer is filled with the culture medium at 37 ℃ and 5% CO2The cells are cultured in the environment of (1) to promote the cells to form cell aggregates, and then the shell layer is removed in a calcium ion removal mode to obtain pure cell aggregates.
Description
Technical Field
The invention relates to a microcarrier suitable for cell growth and a method for culturing the microcarrier in a microenvironment, in particular to a capsule type cell carrier, wherein biodegradable materials are selected, and after cells are cultured into agglomerates, the materials outside the agglomerates can be easily removed to obtain cell agglomerates with cell matrixes for application.
Background
At present, the cell culture system occupies most of the contribution in the self-cell therapy, and can avoid large-scale and high-cost animal experiment resources, however, the traditional cell culture method causes the cells to grow in a monolayer manner, which gradually loses the original characteristics of the cells, such as cell type or protein secretion, and the like, so that the cells are different from the in vivo performance, and the expected effect of the subsequent animal experiment or human body experiment is affected, therefore, the 3D cell culture system provides a cell growth environment more similar to the in vivo environment condition, and can have a better and accurate cell level expected effect. The 3D cell culture environment uses the material with three-dimensional structure for scaffold preparation and cell culture in vitro, which enables cells to grow and migrate in the three-dimensional space structure of the carrier, and the influence is very extensive, such as the interaction and migration between tissue cells, and the influence on the cell morphology, such as cell growth pattern, protein secretion, etc., can be seen in the past many literatures. The purpose of the three-dimensional cell culture environment is to create an environment similar to in vivo growth, thereby helping the growth pattern of cells and intercellular cross-linking to more approximate the in vivo pattern. It is also seen that 3D cell culture systems are expected to grow at 8.7% annual compound growth rate in the future, with the current 3D culture system model having three different disadvantages: 1. hanging drop (hanging drop), large cell mass can not be cultured; 2. cell scaffolds (scaffold), which are highly lost and the removal of material is rather difficult; 3. the hydrogel system (hydrogel system) is a material with poor permeability, so the three modes are not beneficial to the growth of cells.
In addition, when the carrier is prepared by using the hydrogel, if the carrier and the cell mass cannot be effectively separated, the cell mass and the carrier are required to be implanted into the patient, and in this case, the interaction between the cell mass and the adjacent cells is blocked by the carrier, so that the cell mass cannot have good interaction with the adjacent cells after being implanted into the affected part, and the integration effect is further achieved. In addition, the use of water gel also requires the influence on cells after chemical crosslinking with a crosslinking agent.
In addition, after cell masses are formed in the carrier or the support, the carrier prepared by using the polymer material is not easy to remove and cannot be effectively separated from the cell masses, the cell masses and the carrier are implanted into an affected part together, so that the cell masses and cells near the affected part are relatively poor in cell combination and cell integration effect, and the cell treatment effect is further reduced.
Therefore, it is an object of the present invention to provide a material capable of increasing cell adhesion, allowing cells to be completely adhered and cultured in a carrier, and removing unnecessary materials in the direction of cell aggregates, thereby obtaining cell aggregates with cell matrix and integrating the cell aggregates with adjacent cells after injection into an affected part.
Disclosure of Invention
In view of the above, the inventor of the present invention has made various experiments and careful evaluation on the above-mentioned objects in the manufacturing and development experience of related products for many years, and finally has obtained a practical invention.
The present invention has been made in view of the above problems, and it is an object of the present invention to provide a microcarrier suitable for cell growth and a method for culturing the microcarrier in a microenvironment, which can culture cells in the carrier, and can completely attach and culture the cells in the carrier by selecting a material capable of increasing the degree of cell attachment in the carrier, and can be developed in the direction of cell aggregates, thereby finally forming cell aggregates having a cell matrix and being capable of easily removing unnecessary materials, and thus being used for subsequent treatment.
After the cells form the cell mass with the cell matrix in the carrier, the unnecessary shell is removed to obtain the cell mass with the cell matrix and the cell mass is implanted into the affected part to be treated, so that the cell mass can be successfully integrated with the adjacent cells after being injected into the affected part, and the cell mass and the adjacent cells can be well interacted by the method to achieve good cell integration, therefore, the following factors need to be considered when the cell culture environment is performed:
1. high biological activity: when the biological material which is relatively loved and attached by cells is selected as the material in the carrier, the interaction between the cells can be effectively stimulated, and then the proliferation and differentiation of the cells are helped, so that the growth condition of the cells in the carrier is good, and the cells are favorably formed into cell masses.
2. Stability: the degradation and other conditions affect the formation of cell aggregates in the carrier by the cells.
3. Material cells can be differentiated: after the cell mass with the cell matrix is formed, when the cell mass is taken out and implanted into an affected part in vivo, the interaction between the cell mass and adjacent cells becomes a key factor, and the interaction between the cell mass and the adjacent cells can be effectively increased by using a material which can separate the carrier from the cell mass by simple steps.
In view of the above, the present application provides a capsule cell carrier with a putamen structure suitable for three-dimensional cell culture, wherein the material is biodegradable, which is beneficial to easily remove unnecessary external materials after the cells are cultured into clumps, so as to obtain cell clumps with cell matrix and directly apply the cell clumps, and the material located in the inner core can effectively promote the cells to grow and differentiate better, and the capsule cell carrier with the putamen structure can exhibit better effect clinically in the application of three-dimensional cell culture.
A microcarrier suitable for cell growth, comprising a plurality of cells; a carrying solution for carrying the plurality of cells and mixing the carrying solution to form a plurality of inner cores, wherein each inner core is provided with a plurality of cells; one is an outer shell layer of alginic acid, and is coated on the periphery of each inner core, and then the alginic acid and calcium chloride solution are mixed for mild crosslinking to form a plurality of porous microcarriers.
In one embodiment of the present invention, the carrier solution is a degradable biomaterial, and can be gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L ac Acid-co-Glycolic Acid), P L GA), hydroxymethylchitin.
In one embodiment of the present invention, the alginic acid may be further extended by gardenia, or other biopolymer and chemical cross-linking agent.
In one embodiment of the present invention, the degradable biomaterial is a natural polymer or a synthetic polymer extension.
A method of culturing microcarriers in a microenvironment, comprising:
step 1, placing a carrying solution into a first injector, simultaneously adding a plurality of cells and mixing, so that the cells can be carried on the carrying solution to form a plurality of inner cores;
step 2, placing the cores into a first container, and simultaneously placing alginic acid into the container, so that the alginic acid can be respectively coated on the cores to form a plurality of microcarriers;
step 3, placing a plurality of microcarriers into a second container with calcium chloride, and obtaining a plurality of cell microcarriers after the calcium chloride and the alginic acid are crosslinked;
step 4, placing the cell microcarrier in a culture medium for culturing, so that the cells in each cell microcarrier grow to form a cluster;
and 5, removing the outer shell layer with calcium chloride and alginic acid from the pellet in a calcium ion removal mode to obtain a pure cell pellet.
In one embodiment of the present invention, the carrier solution is a degradable biomaterial, and can be gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L ac Acid-co-Glycolic Acid), P L GA), hydroxymethylchitin.
In one embodiment of the present invention, the alginic acid may be further extended by gardenia, or other biopolymer and chemical cross-linking agent.
In one embodiment of the present invention, the culture is performed in a medium under a reaction temperature of 25 ℃ to 39 ℃ with an optimum temperature of 37 ℃.
In one embodiment of the invention, the medium is cultured in a medium in an environment of carbon dioxide (CO)2) Is 5% environment.
In one embodiment of the present invention, the degradable biomaterial is a natural polymer or a synthetic polymer extension.
In one embodiment of the invention, the calcium ion removal is by adding an anticoagulant and sodium citrate to remove the outer shell.
Drawings
FIG. 1 is a schematic representation of a microcarrier according to the invention suitable for cell growth;
FIG. 2 is a flow chart of a method for culturing the microcarrier in the microenvironment;
FIG. 3 is a schematic diagram of the cultivation of a microcarrier suitable for cell growth according to the invention;
FIG. 4 is a culture microscopic analysis view of the microcarrier suitable for cell growth according to the invention;
FIG. 5 is a diagram showing the state of cell growth of the microcarrier suitable for cell growth according to the present invention;
FIG. 6 is an environmental microscopic analysis of a microcarrier suitable for cell growth according to the invention;
FIG. 7 is a diagram showing the difference in environmental growth of microcarriers suitable for cell growth according to the present invention.
Description of the symbols:
(110) loading solution
(120) Multiple cells
(130) Multiple kernels
(140) Outer shell layer
(150) Calcium chloride solution
(160) Microcarrier
(S210-S250) flow
Detailed Description
To facilitate understanding of the technical features, contents, advantages and effects achieved by the present invention, the present invention will be described in detail with reference to the accompanying drawings in the form of embodiments, wherein the drawings are used for illustration and assistance of the specification, and are not necessarily the actual proportion and the precise configuration after the implementation of the present invention, and therefore, the drawings should not be read and the scope of the right of the actual implementation of the present invention should not be limited by the proportion and the configuration of the drawings.
Referring to fig. 1 and 3, a microcarrier for cell growth according to the present invention is shown, and includes a plurality of cells (120), a carrier solution (110) for carrying the cells (120), and mixing the carrier solution to form a plurality of inner cores (130), wherein the carrier solution (110) is a degradable biomaterial and is selected from gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L active-co-Glycolic Acid), P L GA), hydroxymethylchitin, etc., wherein the degradable biomaterial is a natural polymer and a synthetic polymer, an outer shell layer (140) of alginic Acid is coated on the outer periphery of each inner core (130), and is mildly crosslinked by alginic Acid and calcium chloride solution (150) to form a plurality of porous microcarriers (160), and other chemically crosslinked alginic Acid or other polymer carriers are selected from the group consisting of alginic Acid and other chemical additives.
It can be seen from the above description, please refer to fig. 4, which is a culture microscope analysis diagram of the microcarrier suitable for cell growth according to the present invention, the microcarrier suitable for cell growth is analyzed by a Scanning Electron Microscope (SEM), and compared with the microcarrier at 25 ℃, the microcarrier at 37 ℃ has a core in which gelatin is gradually dissolved, so that the microcarrier successfully forming a shell-core structure can verify the potential of cell encapsulation, and meanwhile, the microcarrier is a cell carrier with high biocompatibility and can be used for three-dimensional cell culture, and the microcarrier is a porous carrier to facilitate cell growth. The alginic acid is used as the outer shell layer of the inner core, the culture solution is mixed with gelatin to be used as the inner core, so that the material is favorable for cell adhesion, the alginic acid is contacted and mixed with a calcium chloride solution and then immediately and mildly crosslinked, an isolated environment outside the cell culture can be provided, so that the cells can be cultured in a microcarrier, the cells can grow in the microcarrier and gradually aggregate into lumps, and finally the alginic acid is removed in a calcium removal mode to obtain a cell lump with a cell matrix inside and is implanted into an affected part to assist cell treatment of the affected part.
Referring to FIG. 2, a flow chart of a method for culturing the microcarrier in the microenvironment according to the invention is shown, which comprises:
step 1, (S210) placing the carrying solution into a first syringe, adding a plurality of cells simultaneously, and mixing them to allow the plurality of cells to be carried on the carrying solution to form a plurality of cores;
step 2, (S220) placing the cores in a first container, and simultaneously placing an alginic acid in the container, so that the alginic acid can be coated on the cores respectively to form a plurality of micro-carriers;
step 3, (S230) placing the microcarriers in a second container containing calcium chloride, and cross-linking the calcium chloride with alginic acid to obtain a plurality of cell microcarriers;
step 4, (S240) placing the cell microcarriers in a culture medium for culturing, so that the cells in each cell microcarrier grow to form clumps;
step 5, (S250) removing the outer shell layer containing calcium chloride and alginic acid from the pellet by calcium ion removal to obtain a pure cell pellet.
According to the step 1, the carrying solution is a degradable biomaterial, such as gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L active-CO-Glycolic Acid), P L GA), and hydroxymethylchitin, and the degradable biomaterial is an extension of natural polymer and synthetic polymer, the alginic Acid in the step 2 is an extension of gardenia or other biopolymer and chemical cross-linker, the carrier solution in the step 4 is cultured in a culture medium, the reaction temperature of the culture environment is 37 ℃ plus or minus 0.5 ℃, the optimal temperature is 37 ℃, and the culture environment is carbon dioxide (CO)2) In 5% environment, the calcium ion removal method described in step 5 is to add anticoagulant and sodium citrate, thereby removing the shell layer with calcium chloride and alginic acid.
Therefore, according to the method of micro-environment culture described above, referring to fig. 5, the cell microcarriers are placed in the culture medium for culture, and at a fixed temperature of 37 ℃ and corresponding cell culture solution is replaced for different cells, the cells in each cell microcarrier gradually grow to form clumps, such as the growth state of the cells on days 3, 7 and 14 in fig. 5, and the cell clumps with cell matrix can be formed continuously until day 14.
In contrast to the temperature of the culture environment, the cell microcarrier has the same temperature difference in the process of transporting the cell microcarrier to the environment, as shown in fig. 6 and 7, which are an environmental microscopic analysis chart and an environmental growth difference chart of the microcarrier suitable for cell growth of the present invention, in the current cell carrying manner, when the cell is carried by the freezing tube in the control group, the cell needs to be thawed and thawed first, and then injected into the affected part, and the frozen ice crystal problem may cause a certain degree of cell death (as shown in fig. 6 and 7), so the cell survival rate is not good, and similarly, in the case of a low temperature of 4 ℃ in the refrigerator in another control group, the cell cannot perform endocytosis due to the fact that the cell cannot perform energy consumption, and the cell death condition is obviously caused after 3 days (as shown in fig. 6 and 7), in contrast, the microcarrier of the invention can not only prevent cell death but also proliferate in a proper culture medium environment at room temperature of 25 ℃, so that the microcarrier of the invention can obtain the optimal cell survival rate at the temperature of 25 ℃ to 39 ℃ which is the normal room temperature, wherein 37 ℃ is the optimal temperature.
In summary, the material used in the present invention is biodegradable, and the main purpose of the present invention is to provide a good cell growth environment for the inner core material, so that the inner core material can proliferate and differentiate at the same time in a manner close to the real physiological status of the human body, while the outer shell material provides a good protective layer for the capsule cell carrier, thereby preventing the cell material of the inner core from losing and dying. Meanwhile, the invention can provide the growth condition of cells in a three-dimensional culture environment, reduce and even avoid the loss and the loss of cell materials in the culture process, and further can achieve better effect and success rate when the cell mass is applied.
As can be seen from the above description, the present invention has the following advantages compared with the prior art and the product:
1. the method and the equipment for removing the pollutants by using the mixed bacterial liquid to promote the electrodynamic force use the alginic acid and the calcium chloride which can be mildly crosslinked for physical crosslinking to form a stable cell carrier.
2. According to the method and the equipment for removing the pollutants by using the mixed bacterial liquid to promote the electrodynamic force, the biodegradable material of the inner core can improve the cell attaching capability so as to assist the formation of cell aggregates.
3. The method and the device for removing pollutants by using mixed bacteria liquid to promote electric force can effectively separate the outer shell layer of the cell carrier from the cell mass by removing calcium so as to obtain the pure cell mass with cell matrix.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A microcarrier suitable for cell growth, comprising:
a plurality of cells (120);
a loading solution (110) for loading the plurality of cells (120) and mixing them to form a plurality of cores (130);
an outer shell (140) of alginic acid is coated on the periphery of each of the inner cores (130), and is mildly crosslinked by mixing the alginic acid with a calcium chloride solution (150) to form a plurality of microcarriers (160) having a plurality of pores.
2. The microcarrier of claim 1, wherein the carrier solution (110) is a biodegradable biomaterial selected from the group consisting of gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L ac-co-Glycolic Acid), P L GA), and hydroxymethylchitin.
3. The microcarrier of claim 1, wherein the alginic acid is further extended by gardenia, other biopolymer and chemical cross-linker.
4. The microcarrier for cell growth according to claim 2, wherein the degradable biomaterial is a natural polymer or a synthetic polymer extension.
5. A method for culturing a microcarrier in a microenvironment, comprising:
step 1, (S210) placing the carrying solution into a first syringe, adding a plurality of cells simultaneously, and mixing them to allow the plurality of cells to be carried on the carrying solution to form a plurality of cores;
step 2, (S220) placing the cores in a first container, and simultaneously placing an alginic acid in the container, so that the alginic acid can be coated on the cores respectively to form a plurality of micro-carriers;
step 3, (S230) placing the microcarriers in a second container containing calcium chloride, and cross-linking the calcium chloride with alginic acid to obtain a plurality of cell microcarriers;
step 4, (S240) placing the cell microcarriers in a culture medium for culturing, so that the cells in each cell microcarrier grow to form clumps;
step 5, (S250) removing the outer shell layer containing calcium chloride and alginic acid from the pellet by calcium ion removal to obtain a pure cell pellet.
6. The method of claim 5, wherein the carrier solution is a biodegradable biomaterial selected from gelatin, collagen, hyaluronic Acid, chitin, fibrin, polyglycolic Acid (Poly (Glycolic Acid), PGA), Glycolic Acid copolymer (Poly (L ac Acid-co-Glycolic Acid), P L GA), and hydroxymethyl chitin.
7. The method of claim 5, wherein the alginic acid is further extended by gardenia jasminoides, other biopolymers and chemical crosslinkers.
8. The method of claim 5, wherein the culturing is performed in a culture medium at a reaction temperature of about 37 ℃ plus or minus 0.5 ℃, wherein the optimal temperature is about 37 ℃.
9. The method of claim 5, wherein the culturing is performed in a medium in which carbon dioxide (CO) is used as the culturing environment2) Is 5% environment.
10. The method of claim 6, wherein the degradable biomaterial is an extension of natural polymer or synthetic polymer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010231537.4A CN111440757A (en) | 2020-03-27 | 2020-03-27 | Microcarrier suitable for cell growth and method for culturing microcarrier in microenvironment |
| TW110102812A TWI773101B (en) | 2020-03-27 | 2021-01-26 | Microcarrier suitable for cell growth and method for microcarrier culture in microenvironment |
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| CN202010231537.4A CN111440757A (en) | 2020-03-27 | 2020-03-27 | Microcarrier suitable for cell growth and method for culturing microcarrier in microenvironment |
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| CN111440757A true CN111440757A (en) | 2020-07-24 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114058569A (en) * | 2021-11-19 | 2022-02-18 | 博格隆(浙江)生物技术有限公司 | Animal cell culture microcarrier and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI802291B (en) * | 2022-02-23 | 2023-05-11 | 國立清華大學 | Highly compressible shape memory double network hydrogel, use and preparation method thereof, and intervertebral disk scaffold |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897995A (en) * | 2010-07-09 | 2010-12-01 | 深圳市北科生物科技有限公司 | Implantable membrane-covering three-dimensional carrier and preparation method thereof |
| CN106039421A (en) * | 2015-04-07 | 2016-10-26 | 四川蓝光英诺生物科技股份有限公司 | Biobrick including endothelial cells and application thereof |
| CN108310467A (en) * | 2018-04-17 | 2018-07-24 | 华中科技大学同济医学院附属协和医院 | A kind of packaging cell-derived extracellular matrix membrane composite bone repairing material and its preparation method and application |
-
2020
- 2020-03-27 CN CN202010231537.4A patent/CN111440757A/en active Pending
-
2021
- 2021-01-26 TW TW110102812A patent/TWI773101B/en active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897995A (en) * | 2010-07-09 | 2010-12-01 | 深圳市北科生物科技有限公司 | Implantable membrane-covering three-dimensional carrier and preparation method thereof |
| CN106039421A (en) * | 2015-04-07 | 2016-10-26 | 四川蓝光英诺生物科技股份有限公司 | Biobrick including endothelial cells and application thereof |
| CN108310467A (en) * | 2018-04-17 | 2018-07-24 | 华中科技大学同济医学院附属协和医院 | A kind of packaging cell-derived extracellular matrix membrane composite bone repairing material and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| AHASAN HABIB ET AL.: "3D printability of alginate-carboxymethyl cellulose hydrogel", 《MATERIALS(BASEL)》 * |
| 黎铭峰等: "基于海藻酸钠的组织工程水凝胶纤维丝的成形工艺研究", 《航天医学与医学工程》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114058569A (en) * | 2021-11-19 | 2022-02-18 | 博格隆(浙江)生物技术有限公司 | Animal cell culture microcarrier and preparation method thereof |
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| TWI773101B (en) | 2022-08-01 |
| TW202136499A (en) | 2021-10-01 |
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Application publication date: 20200724 |