CN103947545A - Breeding method of high-yield corn seeds - Google Patents
Breeding method of high-yield corn seeds Download PDFInfo
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- CN103947545A CN103947545A CN201410200174.2A CN201410200174A CN103947545A CN 103947545 A CN103947545 A CN 103947545A CN 201410200174 A CN201410200174 A CN 201410200174A CN 103947545 A CN103947545 A CN 103947545A
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Abstract
The invention provides a breeding method of high-yield corn seeds, aiming at solving the problems that the acre yield of the existing corn variety cannot reach 1000kg, and a technical prejudice that colchicine is only used on the single-rod corn seeds exists in the prior art. The breeding method comprises the steps of soaking the corn seeds in a Na family 778 diluent, and applying enough base fertilizer before sowing; carrying out topdressing in the period from germination to growth of 8-10 leaves; carrying out selfing pollination; after corns are mature, soaking the corn seeds in a colchicine diluent for 6-12h. According to the breeding method, the twin-rod recessive gene of the corns is induced by a gene regulator Na family 778, a twin-rod directional breeding effect can be achieved by the corn seeds through the use of a cell stabilizer colchicine, and preferred breeding of the twin-rod gene is highlighted by an eight-generation selfing method, so that the corn seeds can be directionally cultivated, selfed with parents, purified and strengthened, and the gene of the cultivated corn seeds can be maintained to be stable. Finally, the effect of improving the corn yield by at least 30% can be achieved, and the average acre yield is 250-350kg.
Description
Technical field
The invention belongs to corn breeding techniques field, specifically make the breeding method of a kind of high-yield corn seed of corn yield increasing.
Background technology
Development along with agricultural cience and farming techniques, the output of cereal crops also improves constantly, for China's people's livelihood is made huge contribution, wherein corn is as a kind of Main Economic cereal crops, it output always be what people paid close attention to, the existing corn of China is all the kind with country's authorization, as first beautiful 335, Zheng Dan 958 etc., but the corn per mu yield of these kinds is all in 900kg left and right, after great many of experiments, still do not cultivate so far kind more than average yield per mu 1000kg.People only act on colchicine on single excellent plant, do not recognize and can be used on double stick plant yet, it is sick that double stick plant is considered to a kind of corn crop, and because double stick is not of uniform size, spillikin can be fought for nutrient with big stick-means of intimidation causes two rods all can not grow completely; And stalk growth is thin and tall, be not easy to nutrient and carry and easily lodge, be difficult for survival in hazard weather and make yield reducation.
Summary of the invention
The object of this invention is to provide a kind of fruit size of two fringe corns that makes basically identical, and be not less than the breeding method of the high-yield corn seed of single ear corn.
Technical scheme of the present invention is:
A, first conventional corn seed is soaked to 10-12 hour by Na Shi 778 dilutions, naturally dry rear sowing, sow 2500-3500 pearl for every mu; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 6-12 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, until selfing, be pollinated after the ripe results of corn, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item, until selfing, be pollinated after the ripe results of corn, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Described Na Shi 778 dilution processing methods are: in former medicine, first add 100 ℃ of boiling water of 10 times of amounts, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution processing method is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Selected conventional corn seed refers to: common one-tenth cooked maize is as seed, and pellet is educated serial seed, steps on extra large serial seed, rich friendly serial seed or Zheng Dan series seed.
the mechanism of action: the present invention is for control the colchicine of stabilizing agent when the breeding as multiploid induction, when cell divides, can make chromosomal kinetochore delay division on the one hand, so two monomer separation of the chromosome having copied, and kinetochore still connects together, form " X " shape chromosome image (being called C-mitosis, i.e. colchicine effect mitosis); Be the spindle fiber fracture that causes metaphase on the other hand, or suppress the formation of spindle, result to anaphase chromosome can not shift to the two poles of the earth, and reassemble into a diploid cell nucleus.At this time, cell strengthens and does not divide, or splits into the daughter cell of an acellular core and one and have the nuclear daughter cell of amphipolyploidy.After the period, the cell that this chromosome number has doubled divides while increasing again, has just formed diploid cell and tissue.Utilize this characteristic to amplify two fringe genes, make its last complete profiling heredity.
Advantage of the present invention is:
Owing to using the own double stick recessive gene of Gene regulation agent Na Shi 778 inducing maizes and use cytotostatic agent colchicine to make corn seed obtain double stick directive breeding effect and adopt the method for eight generations selfing to give prominence to the preferred good child-rearing of double stick gene, can make its directive breeding, selfing parent, purification are held up strong, thereby keep the corn seed stable gene after breeding to get off.Finally obtain the effect that improves at least 30% than existing corn yield, average mu (667 square metres) volume increase 250-350kg, the present invention can make the output of corn bring up to 1100-1200kg.Below in conjunction with accompanying drawing and by example, the present invention is described in further detail, but following example is only the present invention's example wherein, do not represent the rights protection scope that the present invention limits, the scope of the present invention is as the criterion with claim.
Embodiment
embodiment 1
The breeding method of corn seed of the present invention:
A, first select any common one-tenth cooked maize as seed of the present invention, by Na Shi 778 dilutions, soak 10-12 hour, naturally dry rear sowing, sow 2500-3500 pearl for every mu, place is in China Liaoning Province; April 23, to the sowing on May 20, used sufficient base fertilizer, this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, (topdressed general June 7 between July 1, and the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 10-12 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, being pollinated after the ripe results of corn until selfing Chinese south (preferably selecting Hainan Province) in place, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item,, in Chinese Liaoning Province, being pollinated after the ripe results of corn until selfing in place, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, place is in Hainan Province of China, and line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, place, in Chinese Liaoning Province, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, place, in China Liaoning Province, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Two male parents and the maternal two fringe kinds of corn are done to quadrature or reciprocal cross, obtain corn hybrid seed F1 generation, then kind value, thereby the commodity corn that output reaches 1200kg left and right obtained.
Described Na Shi 778 dilutions (Yunnan Province ecological agriculture research institute produces) as induction regulating controlling agent processing method are: 100 ℃ of boiling water that first add 10 times of amounts in former medicine, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution as the processing method of making cell Induction Control stabilizing agent is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Every mu of two fringe maize seeds of sowing in 3000-3500 strain.
embodiment 2
The breeding method of corn seed of the present invention:
A, first pellet is educated to serial seed: pellet educate 90 or pellet educate 80 and soak 10-12 hour by Na Shi 778 dilutions, naturally dry rear sowing, sow 2500-3500 pearl for every mu, place is in China Liaoning Province; April 23, to the sowing on May 20, used sufficient base fertilizer, this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, (topdressed general June 7 between July 1, and the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 8-10 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, being pollinated after the ripe results of corn until selfing Chinese south (preferably selecting Hainan Province) in place, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item,, in Chinese Liaoning Province, being pollinated after the ripe results of corn until selfing in place, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, place is in Hainan Province of China, and line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, place, in Chinese Liaoning Province, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, place, in China Liaoning Province, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Two male parents and the maternal two fringe kinds of corn are done to quadrature or reciprocal cross, obtain corn hybrid seed F1 generation, then kind value, thereby the commodity corn that output reaches 1200kg left and right obtained.
Described Na Shi 778 dilutions (Yunnan Province ecological agriculture research institute produces) as induction regulating controlling agent processing method are: 100 ℃ of boiling water that first add 10 times of amounts in former medicine, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution as the processing method of making cell Induction Control stabilizing agent is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Every mu of two fringe maize seeds of sowing in 3000-3500 strain.
embodiment 3
A, first the seed of stepping on sea series is soaked to 10-12 hour by Na Shi 778 dilutions, naturally dry rear sowing, sow 2500-3500 pearl for every mu, place is in China Liaoning Province; April 23, to the sowing on May 20, used sufficient base fertilizer, this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, (topdressed general June 7 between July 1, and the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 6-8 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, being pollinated after the ripe results of corn until selfing Chinese south (preferably selecting Hainan Province) in place, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item,, in Chinese Liaoning Province, being pollinated after the ripe results of corn until selfing in place, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, place is in Hainan Province of China, and line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, place, in Chinese Liaoning Province, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, place, in China Liaoning Province, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Two male parents and the maternal two fringe kinds of corn are done to quadrature or reciprocal cross, obtain corn hybrid seed F1 generation, then kind value, thereby the commodity corn that output reaches 1200kg left and right obtained.
Described Na Shi 778 dilutions (Yunnan Province ecological agriculture research institute produces) as induction regulating controlling agent processing method are: 100 ℃ of boiling water that first add 10 times of amounts in former medicine, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution as the processing method of making cell Induction Control stabilizing agent is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Every mu of two fringe maize seeds of sowing in 3000-3500 strain.
embodiment 4
A, first the seed of rich friend's series is soaked to 10-12 hour by Na Shi 778 dilutions, naturally dry rear sowing, sow 2500-3500 pearl for every mu, place is in China Liaoning Province; April 23, to the sowing on May 20, used sufficient base fertilizer, this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, (topdressed general June 7 between July 1, and the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 6-12 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, being pollinated after the ripe results of corn until selfing Chinese south (preferably selecting Hainan Province) in place, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item,, in Chinese Liaoning Province, being pollinated after the ripe results of corn until selfing in place, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, place is in Hainan Province of China, and line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, place, in Chinese Liaoning Province, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, place, in China Liaoning Province, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Two male parents and the maternal two fringe kinds of corn are done to quadrature or reciprocal cross, obtain corn hybrid seed F1 generation, then kind value, thereby the commodity corn that output reaches 1200kg left and right obtained.
Described Na Shi 778 dilutions (Yunnan Province ecological agriculture research institute produces) as induction regulating controlling agent processing method are: 100 ℃ of boiling water that first add 10 times of amounts in former medicine, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution as the processing method of making cell Induction Control stabilizing agent is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Every mu of two fringe maize seeds of sowing in 3000-3500 strain.
embodiment 5
A, first by the seed of Zheng Dan series: Zheng Dan 958, by Na Shi 778 dilutions, soak 10-12 hour, naturally dry rear sowing, sow 2500-3500 pearl for every mu, place is in China Liaoning Province; April 23, to the sowing on May 20, used sufficient base fertilizer, this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, (topdressed general June 7 between July 1, and the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 6-12 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, being pollinated after the ripe results of corn until selfing Chinese south (preferably selecting Hainan Province) in place, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item,, in Chinese Liaoning Province, being pollinated after the ripe results of corn until selfing in place, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, place is in Hainan Province of China, and line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, place, in Chinese Liaoning Province, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, place, in China Liaoning Province, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, place, in China Hainan Province, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Two male parents and the maternal two fringe kinds of corn are done to quadrature or reciprocal cross, obtain corn hybrid seed F1 generation, then kind value, thereby the commodity corn that output reaches 1200kg left and right obtained.
Described Na Shi 778 dilutions (Yunnan Province ecological agriculture research institute produces) as induction regulating controlling agent processing method are: 100 ℃ of boiling water that first add 10 times of amounts in former medicine, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution as the processing method of making cell Induction Control stabilizing agent is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
Every mu of two fringe maize seeds of sowing in 3000-3500 strain.
Claims (3)
1. a breeding method for high-yield corn seed, the breeding method of corn seed of the present invention:
A, first conventional corn seed is soaked to 10-12 hour by Na Shi 778 dilutions, naturally dry rear sowing, sow 2500-3500 pearl for every mu; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, gathers in the crops into 2-4 fringe maize seed; By colchicine dilution soaking corn kind 6-12 hour, after naturally drying, be the two fringe maize seeds of the first generation again;
B, the two fringe corn seeds of the first generation are repeated to the operation of a item, until selfing, be pollinated after the ripe results of corn, can gather in the crops into the two fringe maize seeds of the second generation;
C, the two fringe corn seeds of the second generation are repeated to the operation of b item, until selfing, be pollinated after the ripe results of corn, can gather in the crops into the two fringe maize seeds of the third generation;
D, the two fringe corn seeds of the third generation are directly sowed, line space of its sowing strain is: 600cm*30cm; Use sufficient prior to seeding base fertilizer, the ratio of weight and number of this base fertilizer composition is nitrogen 16%, sulphur 12%, fulvic acid ammonium 8, potassium fulvate 8%, middle micro-8%, organic 12% Chemical Mixed Fertilizer, and every mu is dropped into 40-80 kilogram; When emerging to 8-10 blade of growth, to topdress, the total effective ingredient of this fertilizer is 64%, wherein: nitrogen 18%, sulphur 12%, amino acid ammonium 8%, fulvic acid ammonium 8%, middle micro-10%, organic 8%; When having the two fringe of 1-2 pearl, every fringe overlaps a transparent plastic bag selfing and is pollinated, and until selfing, is pollinated after the ripe results of corn, can gather in the crops into two fringe maize seeds of the 4th generation;
E, by the 4th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 5th generation;
F, by the 5th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 6th generation;
G, by the 6th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into two fringe maize seeds of the 7th generation;
H, by the 7th generation two fringe corn seeds repeat the operation of d item, can gather in the crops into male parent or the maternal finished product of two fringe maize seeds of the 8th generation;
Described Na Shi 778 dilution processing methods are: in former medicine, first add 100 ℃ of boiling water of 10 times of amounts, soak and be naturally cooled to after normal temperature for 12-24 hour, then can become Na Shi 778 dilutions after adding 20-30 ℃ of warm water that 5-10 doubly measures to mix;
Described colchicine dilution processing method is: after colchicine stoste is diluted to 500 times of amounts at normal temperatures, can become colchicine dilution.
2. the breeding method of corn seed according to claim 1, is characterized in that: every mu of sowing fringe maize seed in 3000-3500 strain.
3. the breeding method of corn seed according to claim 1, is characterized in that: selected conventional corn seed refers to that pellet educates serial seed, steps on extra large serial seed, rich friendly serial seed or Zheng Dan series seed.
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