CN103945829A - Beauty treatment method, skin care composition, bacterial cell, and dried bacterial cell - Google Patents
Beauty treatment method, skin care composition, bacterial cell, and dried bacterial cell Download PDFInfo
- Publication number
- CN103945829A CN103945829A CN201280056675.7A CN201280056675A CN103945829A CN 103945829 A CN103945829 A CN 103945829A CN 201280056675 A CN201280056675 A CN 201280056675A CN 103945829 A CN103945829 A CN 103945829A
- Authority
- CN
- China
- Prior art keywords
- skin
- bacterium
- antibacterial
- thalline
- useful bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/06—Preparations for styling the hair, e.g. by temporary shaping or colouring
- A61Q5/065—Preparations for temporary colouring the hair, e.g. direct dyes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/10—Preparations for permanently dyeing the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Cosmetics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Provided are: a novel and safe beauty treatment method which can cope with the individual variation of skin environments readily, has little adverse impacts such as adverse side effects, and enables the selective proliferation of a useful bacterium to thereby keep the normal bacterial skin flora healthy; a skin care composition; and a dried bacterium cell which can be used suitably for the production of the skin care composition. A beauty treatment method comprising a step of collecting bacteria inhabiting the surface of the skin of a human body, a step of selectively proliferating at least one useful bacterium that can be proliferated on the surface of the skin of a human body and can improve the health state of the skin among the collected bacteria, and a step of administering a composition containing at least one selected from the group consisting of a cell of the proliferated useful bacterium, a cell component of the useful bacterium and a substance produced by the useful bacterium onto the skin of a human body; and a skin care composition containing a useful bacterium that has been proliferated selectively in the above-mentioned manner and/or a substance produced by the useful bacterium.
Description
Technical field
The present invention relates to and the individual variation of skin environment beauty method irrelevant, that go for everyone and be almost free from side effects etc., protect skin composition, and this protect skin composition manufacture in applicable thalline and dry thalline.
Background technology
The living resident bacterium of skin of about tens of kinds of the known surface at human skin.In the resident bacterium of skin, except existing as the harmful thalline of staphylococcus aureus etc., also exist and keeping useful useful bacterium aspect sound skin condition, be favourable and make resident bacterium keep normal condition for improving the state of skin or skin surface.For example, staphylococcus epidermidis (Staphylococcus epidermidis) plays when well assisting a ruler in governing a country effect maintaining of the moisture-keeping functions of skin, isolation features by generating glycerol, also by generating organic acid, make the surface of skin remain on faintly acid, thereby also produced very large effect (for example,, with reference to non-patent literature 1) in the proliferation that suppresses harmful bacteria.In addition, staphylococcus epidermidis can also generate inhibition harmful bacteria, be the antibacterial peptide of the reproduction of staphylococcus aureus.
In order to prevent the dermatosis such as the infectious disease such as alimentary toxicosis or influenza or acne, the breeding of cleaning and suppress harmful bacteria that is necessary to keep skin surface.Therefore, the abluent such as soap has obtained generally application.Yet, if these abluents of excessive use, when causing the moisturizing ingredients from lossing of the skins such as ceramide, also can cause having killed and wounded useful bacterium and produce counteractive situation.
Each strain shared ratio that exists in the resident flora of skin is subject to the impact of various factors, if be suppressed because some factors make the reproduction of useful bacterium, and the situation that can cause harmful bacteria to be dominant, thus can give rise to diseases.Therefore,, for the object that makes resident soundization of flora of skin, for not affecting useful bacterium, only harmful bacteria is shown to the compound of selectivity bactericidal action or bacteriostasis is studied exploitation.For example; a kind of preparation of Rhizoma Coptidis extract as effective ingredient of usining disclosed in patent documentation 1; it is as only propionibacterium acnes (propionibacterium) the performance useful effect to propagation in the resident bacterium of skin of the resident normal agent of bacterium of skin; and aerobic bacteria is not produced to effect, thereby can safeguard the balance of the resident bacterium of skin and make it in the normal state of health.In patent documentation 2, disclose the resident flora improving agent of a kind of skin, wherein contained the water-soluble saccharides that a plurality of sugar forms with the combination of β glycosidic bond.The ecosystem equilibrium modifier that discloses the resident bacterium of a kind of skin in patent documentation 3, it contains Hinokitiol glycocide as active component, and staphylococcus aureus is played to antibacterial, biocidal efficacies, and makes staphylococcus epidermidis propagation.The ecosystem equilibrium modifier of the resident bacterium of a kind of skin is disclosed in patent documentation 4, it contains one or more extracts of selecting from Ludwigia octovalvis (Jacq.) Raven and screw pine extract and salt brine as active component, staphylococcus epidermidis is not shown to bactericidal action, and staphylococcus aureus is had to sterilization or inhibited proliferation.
Prior art document
Patent documentation
Patent documentation 1: TOHKEMY 2010-202604 communique
Patent documentation 2: TOHKEMY 2008-50322 communique
Patent documentation 3: TOHKEMY 2007-246411 communique
Patent documentation 4: TOHKEMY 2007-153800 communique
Non-patent literature
Non-patent literature 1: out tail lattice, Yuichi Zheng are put down and jointly write, " with 100,000,000 antibacterials of human body symbiosis ", Newton (the Newton press of Co., Ltd. distribution), in November, 2010 number, 68th～75 pages
Summary of the invention
The problem that invention will solve
But, with regard to realize the method for patent documentation 1～4 of resident soundization of flora of skin by dropping into ectogenic compound with regard to, at harmful bacteria, obtain drug resistance or morph because of each strain that forms the resident flora of skin that do not cause on an equal basis of individual's skin environment, thereby while causing that drug-susceptibility changes etc., exist the problem that cannot bring into play soundization effect.And want to change from multiple candidate compound according to these customized while selecting optimum medicament, be often accompanied by and be difficult to the difficulty expected.
In view of the above problems, the invention provides the harmful effects such as a kind of individual variation that can tackle like a cork skin environment, side effect few, and can realize by optionally breeding useful bacterium soundization, the novel and safe beauty method of the resident flora of skin, protect skin composition, and protect applicable dry thalline in skin composition manufacture at this.
The method of dealing with problems
That is, the present invention by provide as described in beauty method as described in following [1]～[7], [8]～[12] protect thalline as described in skin composition, [13]～[17] and the dry thalline as described in [18] has solved the problems referred to above.
[1] beauty method, it has the step of obtaining the antibacterial that the skin surface at human body lives, thereby make in described antibacterial to improve the step that one or more useful bacterium of the health status of skin are optionally bred in the skin surface propagation of human body, will contain one or more compositions in the choosing group that freely the generation material of thalline, thalline composition and the described useful bacterium of the useful bacterium of described propagation forms and put into the step of human body skin.
[2] beauty method as described in above-mentioned [1], wherein, in described input step, to carry out the input (backing up) of described compositions as the same individuality of object when obtaining described antibacterial.
[3] beauty method as described in above-mentioned [1] or [2], wherein, described antibacterial is the resident bacterium of skin.
[4] beauty method as described in any one in above-mentioned [1]～[3], wherein, described in one or more useful bacterium are optionally bred step comprise
The preculture step of the described antibacterial obtaining with culture medium culturing,
From the antibacterial of described cultivation, filter out the screening step of described useful bacterium,
The incubation step of the useful bacterium filtering out described in cultivation.
[5] beauty method as described in any one in above-mentioned [1]～[4], wherein, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
[6] beauty method as described in any one in above-mentioned [1]～[5], wherein, described useful bacterium is coagulase negative staphylococcus.
[7] beauty method as described in above-mentioned [6], wherein, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
[8] protect skin composition, it contains one or more materials in the choosing group that freely generation material of thalline, thalline composition and the described useful bacterium of useful bacterium forms, described useful bacterium be separated and selective proliferative the antibacterial obtaining from the skin surface from human body, can breed and improve at the skin surface of human body one or more useful bacterium of the health status of skin.
[9] as described in above-mentioned [8], protect skin composition, wherein, described antibacterial is the resident bacterium of skin.
[10] as described in above-mentioned [8] or [9], protect skin composition, wherein, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
[11] as described in any one in above-mentioned [8]～[10], protect skin composition, wherein, described useful bacterium is coagulase negative staphylococcus.
[12] as described in above-mentioned [11], protect skin composition, wherein, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
[13] thalline, thus to be one or more useful bacterium of allowing in the antibacterial obtaining at the skin surface from human body to improve the health status of skin in the skin surface propagation of human body optionally breed and the thalline that obtains for it.
[14] thalline as described in above-mentioned [13], wherein, described antibacterial is the resident bacterium of skin.
[15] thalline as described in above-mentioned [13] or [14], wherein, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
[16] thalline as described in any one in above-mentioned [13]～[15], wherein, described useful bacterium is coagulase negative staphylococcus.
[17] thalline as described in above-mentioned [16], wherein, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
[18] dry thalline, its be by as above-mentioned [13]～[17] in thalline as described in any one be dried and must dry thalline.
The effect of invention
With regard to the resident bacterium of antibacterial, especially skin obtaining from human body skin, under the intrinsic skin environment of human body, especially easily breed.Therefore, if by one or more useful bacterium that contain selective proliferative in the antibacterial obtaining at the skin surface from human body or promote the thalline composition of useful bacterium of its propagation or the skin surface that the compositions that generates material is put into human body, by useful bacterium, at skin surface, form advantage, the breeding of harmful bacteria can be effectively suppressed, and the balance of the resident flora of skin can be kept aptly.Its result, can keep or improve the health status of skin effectively.Therefore, beauty method provided by the invention and protect skin composition and can effectively keep sound skin condition.And, owing to not making with medicament, therefore have advantages of that the harmful effects such as safety and side effect are few at beauty method of the present invention and in protecting skin composition.
Particularly, the individual variation of the resident bacterium of the antibacterial obtaining from individual skin, especially skin and skin environment adapts, and under the intrinsic skin environment of this individuality, especially easily breeds.Therefore, in the antibacterial obtaining at the skin surface from individual, only optionally breed useful bacterium, and the useful bacterium of this propagation is dropped into (backing up) after this individual skin surface, useful bacterium will independently form advantage at skin surface with the individual variation of skin environment, thereby can more effectively suppress the breeding of harmful bacteria, and can guarantee more effectively the balance of the resident flora of skin.
Accompanying drawing explanation
Fig. 1 means that the backing up operation of the staphylococcus epidermidis that the resident bacterium of the skin of self is originated is for the figure of the impact of the staphylococcus epidermidis number of skin surface.
Fig. 2 means that the backing up of the staphylococcus epidermidis in the resident bacterium of the skin of self source operates the figure for the impact of epidermis water quantities.
The specific embodiment
Below, embodiments of the present invention (be only example, not delimit the scope of the invention) are described.
(following as the related beauty method of an embodiment of the invention, there is the situation referred to as " beauty method "), there are following steps, , from the skin surface of (individual human) of individuality, obtain the step of the resident bacterium (example for antibacterial) of skin of living on this surface, making can be in individual skin surface propagation in the resident bacterium of skin, thereby the step that one or more useful bacterium of improving the health status of skin are optionally bred, the thalline of the free useful bacterium breeding of choosing will be contained, the skin composition that protects of one or more in the group that the generation material of thalline composition and useful bacterium forms drops into (backing up) to the step of individual's skin.
As the skin composition that protects using in beauty method, one or more in the group that the generation material of thalline, thalline composition and the useful bacterium of one or more useful bacterium that contain separated the antibacterial that choosing freely obtains from the skin surface from individual and selective proliferative forms, and by comprise from individual skin obtain the step of the antibacterial of living on this surface and the antibacterial that obtaining only optionally breed the step of one or more useful bacterium method make.Each step of below, protecting skin composition to manufacturing is carried out more specific description.
(1) antibacterial obtains
Skin surface exists take the antibacterial that the resident bacterium of tens of kinds of skins is representative.The antibacterial of using while protecting skin composition as manufacture, as long as can effectively safeguard sound skin condition, just can use separately in them any one or suitably combine and use two or more arbitrarily.As applicable antibacterial in protecting skin composition manufacture, can enumerate the resident bacterium of skin of human body, as its object lesson, can enumerate staphylococcus epidermidis (Staphylococcus epidermidis).
The various antibacterials such as the resident bacterium of skin are present in the skin surface of whole body, especially on the surface of face or oxter etc., exist in a large number.Therefore, preferably from these positions, obtain.Especially, when as cosmetics, skin composition is protected in use, preferably from the skin surface of face, obtain as the antibacterial of the resident bacterium of skin.The balance of the resident bacterium of skin is because the skin surface healthy has obtained appropriate maintenance, so (perfecting state) skin surface obtaining preferably in health of the resident bacterium of skin carries out.
While obtaining antibacterial, can adopt from skin surface and obtain antibacterial and can the separated method arbitrarily of bacterial strain arbitrarily.For example, can adopt the following method of enumerating,, by the method that the skin surface of utensil wiping arbitrarily such as the swab stick after moistening sterilizing, gauze, non-woven fabrics, paper, sponge obtains in normal saline or buffer as required, by the agar culture medium after sterilizing by being pressed in skin surface, make antibacterial from skin surface, transfer to the method for media surface, by water, physiological saline and so on liquid, contact with skin surface and make bacterial suspension that skin surface exists in liquid, and then the method together reclaiming with liquid etc.Due to strain contained in the antibacterial obtaining and their quantity than be diagnosis skin health status time index, so the preferably certain area on skin that obtains of antibacterial carries out.When obtaining antibacterial by the method for sterilizing swab stick wiping skin surface, area in order to ensure the wiping of sterilizing swab stick is certain, can will by synthetic resin or glass etc., be formed and cylindric, the oval tubular of both ends open or the instrument that obtains of prism-shaped are attached on skin lightly, by the inside of opening is carried out wiping and obtained antibacterial without omitting.By this method, conventionally can from regulation areal extent in skin surface obtain antibacterial.
On the other hand, when adopting when the agar culture medium after sterilizing is obtained to antibacterial by the method that is pressed in skin surface, by use, there is the culture medium of regulation area (radius), conventionally can obtain antibacterial from the skin surface in certain areal extent.For can in the culture medium of obtaining middle use of antibacterial, there is no particular limitation, but as the object lesson of the culture medium of obtaining middle use the resident bacterium of skin, can enumerate conventional viable bacteria and cultivate the selection isolation medium that the standard agar culture medium of use or tryptose soya agar culture medium, egg yolk are used with staphylococcuses such as mannitol Sal culture medium or Sal egg yolk agar mediums.In addition,, because the culture medium of obtaining middle use on antibacterial contacts with skin surface, therefore preferably use the culture medium of making containing the composition that likely becomes the material of anaphylactogen by not.
(2) selective proliferative of useful bacterium
As mentioned above, the antibacterial obtaining from skin surface, both contain useful bacterium and also contained harmful bacteria.Only optionally breed therein one or more desired useful bacterium.As the method for selective proliferative, there is the method for cultivating after useful bacterium of isolating, only breeding the method for cultivating under the condition of desired useful bacterium, can adopt any one method.
As the former example of method, the step of optionally breeding useful bacterium can comprise the step of following (i)～(iii).
(i) in culture medium, cultivate the preculture step of the antibacterial obtaining
(ii) antibacterial of cultivating is identified, thereby filter out the screening step of useful bacterium
(iii) incubation step of the useful bacterium filtering out being cultivated
(i) preculture step
Utilize the diluted such as phosphate buffer normal saline (PBS) for example, to the multiplying power (, 10～100000 times) of regulation the antibacterial obtaining by sterilizing swab stick etc., cultivate after being coated on the media surface of cultivating use.Condition of culture can suitably be set according to the condition of the kind of the useful bacterium as separate object or the culture medium of using etc.
In addition, when when culture medium is obtained to antibacterial by the method that is pressed in skin surface, preculture step can directly be carried out on the surface that has completed the culture medium of obtaining, but also can will with the antibacterial that sterilizing swab stick etc. is obtained again, dilute from media surface, be coated on after the media surface of cultivating use, by carrying out preculture with above-mentioned identical step.As the culture medium of cultivating use, can enumerate selection isolation medium that culture medium that the conventional antibacterials such as tryptose soya agar culture medium (TS culture medium) use or above-mentioned staphylococcus use etc.
(ii) screening step
For the antibacterial of cultivating in the preculture step above-mentioned, carry out the evaluation of strain, thereby filter out useful bacterium.Screening step can adopt known method arbitrarily to carry out, and for example, can carry out according to following step.First, perusal culture medium, counts the clump count forming in culture medium, presorts under possible prerequisite according to outward appearance simultaneously.Subsequently, to each bacterium colony or wherein representational several bacterium colonies carry out the evaluation of strain.For example,, if staphylococcus epidermidis is identified in conjunction with microscopic examination, Gram’s staining and coagulase determination of activity.Especially, different from pathogen such as staphylococcus aureuses, because staphylococcus epidermidis is coagulase-negative (CNS), therefore, be determined at and the distinguishing of staphylococcus aureus of coagulase activity is important means.
(iii) incubation step
As mentioned above, the useful bacterium that screening (separation) goes out from antibacterial such as the resident bacterium of skin are cultivated.Cultivation can adopt known method arbitrarily to carry out.Thus obtained useful bacterium adapts to the environment facies that obtain the individual skin surface of the resident bacterium of skin, therefore join and protect in skin composition and drop into (backing up) after individual skin surface, can breed and form advantage at skin surface, and can obtain the state that improves skin surface correspondingly or suppress the effect of harmful bacteria propagation etc.Situation during with preculture step is identical, and the culture medium of using during cultivation, condition of culture can suitably be set according to the condition of the kind of the useful bacterium as separate object or the culture medium of using etc.In addition, when having filtered out a plurality of useful bacterium, each bacterial strain can be carried out after separation single culture, when protecting skin composition and manufacture, with the ratio of regulation, mix and used, but cultivate under the state that also can mix multiple useful bacterium.
(3) protect the preparation of skin composition
When skin composition is protected in preparation, can directly use by the viable bacteria of the useful bacterium of above-mentioned steps selective proliferative, but because the state containing viable bacteria is difficult to long-term preservation, therefore also can prepare the dry thalline of torpor bacterium or the useful bacterium of useful bacterium, by it is dispersed in moisture excipient, useful bacterium is restored before use, thus the skin composition that protects that configuration contains useful bacterium.
As the excipient that protects skin composition, as long as useful bacterium can survive therein and/or breed, to it, there is no particular limitation, can use the basic cosmetics arbitrarily such as astringent, emulsion, beautifying liquid, gel, emulsifiable paste, lotion, spray, the special care product such as facial film, the scalp care articles for use such as brilliantine, hair care water, hair growth promoter, hairdressing gel, hair conditioner, hair care film, hair dye or hair care apply some make up etc.For the blending amount of the useful bacterium of unit volume, can suitably select according to the kind of excipient and useful bacterium etc.
Manufacture method as dry thalline, can adopt known method arbitrarily, and to it, there is no particular limitation, as its object lesson, can enumerate freeze-drying, hot-air seasoning etc.For example; when adopting freeze-drying to make dry thalline, under the condition coexisting at suitable protective agents such as skim milk or soyabean milk, useful bacterium is dispersed in dispersant (conventionally, making water); utilize liquid nitrogen etc. carry out freezing after, under decompression, utilize freezer dryer to remove moisture.The dry thalline obtaining thus can be encapsulated in sterilizing ampoule bottle etc. in take care of.
In addition, when the product of the dead thalline of useful bacterium, the thalline compositions such as ultrasonic disruption thing of thalline or useful bacterium, verify when useful bacterium has special propagation promotion activity, in protecting skin composition, also can contain the viable bacteria that these substitute useful bacterium.This is due to following reason,, except providing with the viable bacteria of bacterium, replace to individual epidermis and provide when individual skin surface promotes the material of propagation of useful bacterium specifically, also can increase the ratio of useful bacterium shared in the resident flora of skin, and intactly keep the health status of skin.While using the dead thalline of useful bacterium or the generation material of useful bacterium when substituting the viable bacteria of useful bacterium, protect skin composition and can be the shampoo that contains surfactant, bath gel, head Epoxy resins such as abluent for hydrotherapy.
While containing the viable bacteria of useful bacterium in protecting skin composition, under the prerequisite its manufacture and/or propagation not being exerted an influence, in cosmetics etc., can contain the additive arbitrarily conventionally using.As the object lesson of this additive, can enumerate spice, deposit agent, antioxidant, antibacterial (only limiting to does not have the preparation a little less than antibacterial activity or antibacterial activity for useful bacterium), emulsifying agent, stabilizing agent etc.In protecting skin composition, can further contain the material that specificity promotes the propagation of useful bacterium.
By any one or the multiple skin composition that protects in the generation material of thalline, thalline composition and the useful bacterium of the useful bacterium containing obtaining to some extent are dropped into (backing up) to individual skin, thereby useful bacterium is dropped into (backing up) and arrive individual skin (as the position of dropping into (backing up), can the position when obtaining the resident bacterium of skin be same or different positions) upper after, the useful bacterium adapting to the environment facies of this individual skin surface is promptly bred at skin surface, thereby can improve the health status of skin.In this beauty method, as the input method of protecting skin composition, can select smearing of liquors such as astringent, emulsion, lotion, the spraying of spray, emulsifiable paste, gel etc. the method arbitrarily conforming to the character of protecting skin composition such as smear.While dropping into, can carry out with finger etc., but also can adopt the utensils such as aerosol apparatus, applicator to carry out.As protecting the input interval of skin composition and the input amount of every time, can suitably regulate according to health status of the position of the kind of useful bacterium, input, skin etc., but in order to make useful bacterium be attached to effectively skin surface, preferably routinely drop into.
In addition, in the beauty method of present embodiment, in the antibacterial obtaining at the skin from individual, only optionally bred useful bacterium, and the skin composition that protects that contains this useful bacterium is dropped into (backing up) to same individuality, but also can put into the different individual skin surface of individuality when obtaining antibacterial.Or, also can drop into the skin composition that protects of the useful bacterium (can contain or not contain the useful bacterium that derives from the antibacterial obtaining from me) that contains selective proliferative the antibacterial obtaining from a plurality of individualities.
Embodiment
Below, for the embodiment carrying out in order to confirm effect of the present invention, describe.
Embodiment 1: the making of separated staphylococcus epidermidis single culture and dry thalline from the resident bacterium of skin
[1] the resident bacterium of skin obtains
Below operation is to carry out wearing under the state of antimicrobial gloves.
Skin surface by cylinder (the internal diameter 25mm Ф) applying light of the clean Merlon system by pressure cooker sterilizing at experimenter's forehead, is used in the dipping sterilizing swab stick of 20 seconds in sterilized phosphate buffer normal saline (PBS) skin surface that is exposed to the inside of cylinder is carried out to thorough wiping.Remove cylinder, by sterilizing swab stick, wipe away the moisture of bark fetching skin remained on surface.The moisture adhering to for the inner side of the skin contact at cylinder, equally also wipes away and gets by sterilizing swab stick.By these sterilizing swab sticks, (folding falls the part having contacted with picker's hands.) be equipped with in the sterile tube of sterilizing PBS in putting into, after cover lid, be moved in safety cabinet.
[2] preculture
In the bacterium liquid obtaining by above step 100 μ L are added to, be equipped with in the miniature tube of PBS of 900 μ L, after vortex vibration, made 10 times of diluents.With diluent, substitute bacterium liquid and repeat same operation, thereby having made 100～100000 times of diluents.(following in tryptose soya agar culture medium (TS culture medium) and selective medium for staphylococcus aureus, be referred to as " selection culture medium ") in, add respectively obtained each diluent 50 μ L and make it dispersed, at 37 ℃, having cultivated 48 hours.
[3] separation of staphylococcus epidermidis
The bacterium colony that perusal forms in TS culture medium and selection culture medium, and each bacterium colony is numbered.In TS culture medium and selection culture medium, at the condition of culture identical with above-mentioned (2), each bacterium colony has been carried out to single culture.Each bacterium colony forming is numbered, on microscope slide, drips deionized water, the thalline of using platinum filament from bacterium colony picking is disperseed, with blowtorch, toast overleaf, thereby utilize flame fixation thalline to be fixed on to the surface of microscope slide.With crystal violet solution, iodine liquid, husky yellow solution, process successively, thereby carried out Gram’s staining.Subsequently, for thalline contained in each bacterium colony, by microscopic examination, dyeability and form have been confirmed.Meanwhile, utilizing API STAPH flat-panel systems to carry out confirming as after the evaluation of strain is staphylococcus epidermidis.
[4] single culture of staphylococcus epidermidis and the making of dry thalline
From be accredited as the bacterium colony of staphylococcus epidermidis, obtain thalline, in TS culture medium, carried out single culture.With platinum loop picking thalline (bacterium colony of a 1mm diameter) from the bacterium colony obtaining, be dispersed in after in protectant skim milk and be injected into ampoule bottle, in-80 ℃ of ultralow cold storages, carried out quick freezing.Subsequently, with freezer dryer, process and obtained dry thalline powder.
[5] recovery of dry thalline
Will contain ampoule bottle Kaifeng of dry thalline, add a small amount of liquid TS culture medium dry thalline that suspends.The suspension obtaining is joined respectively in liquid TS culture medium, flat TS culture medium and mannitol culture medium, at 37 ℃, cultivated after 48 hours, from each culture medium, thalline can be detected, confirmed to have formed bacterium colony simultaneously on plating medium.These thalline are identified, confirming as is staphylococcus epidermidis.
Embodiment 2: clinical trial
21 women of 20～50 age brackets are as experimenter, first day in test, the resident bacterium of skin that skin from me (forehead) is obtained has carried out separation and single culture with the step described in embodiment 1, thereby has obtained the staphylococcus epidermidis of the resident bacterium of skin that derives from self.To experimenter, drop into after (backing up), on the staphylococcus epidermidis number of skin surface and discuss for the impact of epidermis water quantities.
21 experimenters are divided into 13 the 1st group (in Fig. 1 and Fig. 2, be labeled as " skin Caring bacterium backing up group ") and 8 the 2nd group (in Fig. 1 and Fig. 2, be labeled as " blank group ") (not informing which group experimenter has been in), and distributed basic cosmetics (nonalcoholic astringent (HORIN company system), Hydra gel (HORIN company system) and emulsion (HORIN company system)).Meanwhile, to the 1st component, sent out the dry thalline (following, referred to as " dry thalline ") of the staphylococcus epidermidis of the resident bacterium of skin that derives from me, given the 2nd group and distributed placebo (skim milk).Experimenter for each group, in 4 time-of-weeks, at the skin of face, smear astringent successively, be suspended with dry thalline (in the each use amount of gel, for approximately 1,000,000,000) or Hydra gel, the emulsion of placebo, thereby regularly (first day in each week and the 4th day) implemented that input (backing up) derives from the operation of staphylococcus epidermidis of the resident bacterium of skin of self or the operation that drops into placebo (in Fig. 1, is labeled as " backing up of skin Caring bacterium ".Below, be generically and collectively referred to as " backing up operation ").In order to inquire into the impact of backing up operation, regular (1 time weekly) has measured staphylococcus epidermidis number and the epidermis water quantities of the skin surface of forehead.In the mensuration of staphylococcus epidermidis number, in the method [1] by embodiment 1, [2] described from the skin surface of forehead obtains and the skin of cultivating reside in the bacterium colony of bacterium, utilize [3] described method of embodiment 1 to identify the bacterium colony of staphylococcus epidermidis, count subsequently.In the latter's mensuration, Cutometer MPA580 (Cutometer, German C+K company system) and Corneometer probe (be above the 1st take turns disposal) have been used.
The 1st, take turns in 4 time-of-weeks of disposing after finishing, the experimenter of the 2nd group of only take is object, except substituting placebo, become the dry thalline of distribution, taking turns and dispose the backing up operation of staphylococcus epidermidis that identical condition has carried out deriving from the resident bacterium of skin of self with the 1st.In order to inquire into the impact of backing up operation, regularly (every two weeks) have measured the staphylococcus epidermidis quantity of the skin surface of forehead.For the experimenter of the 1st group, the 1st take turns dispose finish after and during through 4 time-of-week, measured the staphylococcus epidermidis quantity (be above the 2nd take turns disposal) of the skin surface of forehead.
In Fig. 1, illustrated each group staphylococcus epidermidis quantity before clinical trial starts be made as 1 o'clock, staphylococcus epidermidis quantity (being labeled as " skin Caring bacterium relative populations ") over time.In the 1st group, the staphylococcus epidermidis quantity of skin surface significantly increases dropping into while (backing up) staphylococcus epidermidis of the resident bacterium of skin that derives from self, but take turns to dispose the 1st, finishes and stop to become when backing up operates minimizing.On the other hand, in the 2nd group, with respect to be roughly certain situation between the input time of placebo, when being switched to backing up and deriving from the operation of staphylococcus epidermidis of the resident bacterium of skin of self, can observe the phenomenon that the staphylococcus epidermidis quantity of skin surface significantly increases.These results show, (1) by dropping into (backing up), derive from the staphylococcus epidermidis of the resident bacterium of skin of self, staphylococcus epidermidis can be attached to skin surface, (2) effectively because the staphylococcus epidermidis quantity of skin surface reduces after backing up EO, therefore preferably proceeds to drop into (backing up operation).
In Fig. 2, illustrated the 1st group and the 2nd group and taken turns the 1st the epidermis water quantities (moisture of skin relative value) of disposing the forehead before and after finishing.Can confirm thus, by taking turns the 1st the operation of staphylococcus epidermidis that the input of 3～4 weeks by a definite date (backing up) in disposal derives from the resident bacterium of skin of self, the epidermis water quantities of the 1st group significantly increases.On the other hand, observe the 2nd group and be roughly certain situation in the 1st epidermis water quantities of taking turns in disposal process.
These presentation of results, by the staphylococcus epidermidis that derives from the resident bacterium of skin of self is dropped into (backing up) to the skin of self, staphylococcus epidermidis can be in the resident flora of skin formation advantage, the epidermis water quantities as an index of the health status of skin is improved thereupon.
Claims (18)
1. beauty method, is characterized in that, has
Obtain the step of the antibacterial that the skin surface at human body lives,
Thereby make in described antibacterial to improve the step that one or more useful bacterium of the health status of skin are optionally bred in the skin surface propagation of human body,
The compositions of one or more in the group that the generation material of thalline, thalline composition and the described useful bacterium of the useful bacterium that contains the free described propagation of choosing is formed is put into the step of human body skin.
2. beauty method as claimed in claim 1, is characterized in that, in described input step, to carry out input, the backing up of described compositions as the same individuality of object when obtaining described antibacterial.
3. beauty method as claimed in claim 1 or 2, is characterized in that, described antibacterial is the resident bacterium of skin.
4. the beauty method as described in any one in claim 1～3, is characterized in that, described in one or more useful bacterium are optionally bred step comprise
The preculture step of the described antibacterial obtaining with culture medium culturing,
From the antibacterial of described cultivation, filter out the screening step of described useful bacterium,
The incubation step of the useful bacterium filtering out described in cultivation.
5. the beauty method as described in any one in claim 1～4, is characterized in that, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
6. the beauty method as described in any one in claim 1～5, is characterized in that, described useful bacterium is coagulase negative staphylococcus.
7. beauty method as claimed in claim 6, is characterized in that, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
8. protect skin composition, it is characterized in that, contain one or more materials in the choosing group that freely the generation material of thalline, thalline composition and the described useful bacterium of useful bacterium forms, described useful bacterium be separated and selective proliferative the antibacterial obtaining from the skin surface from human body, can breed and improve at the skin surface of human body one or more useful bacterium of the health status of skin.
9. the skin composition that protects as claimed in claim 8, is characterized in that, described antibacterial is the resident bacterium of skin.
10. protect as claimed in claim 8 or 9 skin composition, it is characterized in that, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
11. protect skin composition as described in any one in claim 8～10, it is characterized in that, described useful bacterium is coagulase negative staphylococcus.
12. skin compositions that protect as claimed in claim 11, is characterized in that, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
13. thalline, thus to be one or more useful bacterium of allowing in the antibacterial obtaining at the skin surface from human body to improve the health status of skin in the skin surface propagation of human body optionally breed and the thalline that obtains for it.
14. thalline as claimed in claim 13, is characterized in that, described antibacterial is the resident bacterium of skin.
15. thalline as described in claim 13 or 14, is characterized in that, described antibacterial is the antibacterial that the skin surface that perfects state at human body lives.
16. thalline as described in any one in claim 13～15, is characterized in that, described useful bacterium is coagulase negative staphylococcus.
17. thalline as claimed in claim 16, is characterized in that, described useful bacterium is staphylococcus epidermidis (Staphylococcus epidermidis).
18. dry thalline, it is the thalline as described in any one in claim 13～17 to be dried and the dry thalline that obtains.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011253056 | 2011-11-18 | ||
| JP2011-253056 | 2011-11-18 | ||
| PCT/JP2012/078835 WO2013073431A1 (en) | 2011-11-18 | 2012-11-07 | Beauty treatment method, skin care composition, bacterial cell, and dried bacterial cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103945829A true CN103945829A (en) | 2014-07-23 |
| CN103945829B CN103945829B (en) | 2016-08-24 |
Family
ID=48429494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280056675.7A Active CN103945829B (en) | 2011-11-18 | 2012-11-07 | Beauty method, protect skin composition, thalline and dry thalline |
Country Status (4)
| Country | Link |
|---|---|
| JP (2) | JP5584833B2 (en) |
| KR (1) | KR101406808B1 (en) |
| CN (1) | CN103945829B (en) |
| WO (1) | WO2013073431A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113413327A (en) * | 2021-06-21 | 2021-09-21 | 山东焦点福瑞达生物股份有限公司 | Preparation method of polysaccharide skin microecological cosmetic |
| CN114058559A (en) * | 2022-01-17 | 2022-02-18 | 山东锦鲤生物工程有限公司 | Staphylococcus epidermidis and application thereof |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102587334B1 (en) * | 2015-06-30 | 2023-10-12 | (주)아모레퍼시픽 | Strains for improving skin, and kit for improving skin using the same |
| JP2019510036A (en) | 2016-03-31 | 2019-04-11 | ゴジョ・インダストリーズ・インコーポレイテッド | A detergent composition comprising probiotic / prebiotic active ingredients |
| US10806769B2 (en) | 2016-03-31 | 2020-10-20 | Gojo Industries, Inc. | Antimicrobial peptide stimulating cleansing composition |
| AU2017365019A1 (en) | 2016-11-23 | 2019-07-11 | Gojo Industries, Inc. | Sanitizer composition with probiotic/prebiotic active ingredient |
| JP7208894B2 (en) * | 2017-05-23 | 2023-01-19 | 森永乳業株式会社 | Cosmetics and skin protection agents containing lactic acid bacteria |
| JP7219033B2 (en) * | 2018-09-14 | 2023-02-07 | ポーラ化成工業株式会社 | M. Skin external composition containing aerilata as an active ingredient |
| KR102305609B1 (en) * | 2019-08-27 | 2021-09-28 | 코스맥스 주식회사 | A genus of Staphylococcus strain and uses thereof |
| JP2021155370A (en) * | 2020-03-27 | 2021-10-07 | 株式会社エルピダ | Cosmetic, cosmetic composition, and method for producing cosmetic composition |
| JP7546995B1 (en) | 2024-03-29 | 2024-09-09 | 株式会社バイオジェノミクス | Bacteria, gene expression enhancers and preparations |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06135843A (en) * | 1992-10-23 | 1994-05-17 | Sagami Chem Res Center | Agent for skin |
| JPH06271851A (en) * | 1993-03-19 | 1994-09-27 | Narisu Keshohin:Kk | Antioxidant and cosmetic |
| JP2005304363A (en) * | 2004-04-20 | 2005-11-04 | Hakuto Co Ltd | Anti-oxidizing substance secretion-promoting agent and method for producing anti-oxidizing substance |
| JP2006219414A (en) * | 2005-02-10 | 2006-08-24 | Showa Denko Kk | External preparation for skin, method for preventing adhesion of microorganism harmful to skin by using the same and method for preventing proliferation |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0637384B2 (en) * | 1985-09-21 | 1994-05-18 | 株式会社資生堂 | Skin cosmetics |
| JPH0977653A (en) * | 1995-09-14 | 1997-03-25 | Advance Co Ltd | Cosmetic |
| JP3608059B2 (en) * | 1995-12-05 | 2005-01-05 | 株式会社アドバンス | Cosmetics |
| JP2000128783A (en) * | 1998-10-23 | 2000-05-09 | Lion Corp | Gram-negative bacterium-resistant agent |
| JP2003321341A (en) * | 2002-05-07 | 2003-11-11 | Sankodo:Kk | Dermatologic medicine |
-
2012
- 2012-01-20 KR KR1020120006990A patent/KR101406808B1/en active IP Right Grant
- 2012-11-07 WO PCT/JP2012/078835 patent/WO2013073431A1/en active Application Filing
- 2012-11-07 JP JP2013541091A patent/JP5584833B2/en active Active
- 2012-11-07 CN CN201280056675.7A patent/CN103945829B/en active Active
-
2014
- 2014-06-20 JP JP2014127664A patent/JP6023756B2/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06135843A (en) * | 1992-10-23 | 1994-05-17 | Sagami Chem Res Center | Agent for skin |
| JPH06271851A (en) * | 1993-03-19 | 1994-09-27 | Narisu Keshohin:Kk | Antioxidant and cosmetic |
| JP2005304363A (en) * | 2004-04-20 | 2005-11-04 | Hakuto Co Ltd | Anti-oxidizing substance secretion-promoting agent and method for producing anti-oxidizing substance |
| JP2006219414A (en) * | 2005-02-10 | 2006-08-24 | Showa Denko Kk | External preparation for skin, method for preventing adhesion of microorganism harmful to skin by using the same and method for preventing proliferation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113413327A (en) * | 2021-06-21 | 2021-09-21 | 山东焦点福瑞达生物股份有限公司 | Preparation method of polysaccharide skin microecological cosmetic |
| CN114058559A (en) * | 2022-01-17 | 2022-02-18 | 山东锦鲤生物工程有限公司 | Staphylococcus epidermidis and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101406808B1 (en) | 2014-06-12 |
| WO2013073431A1 (en) | 2013-05-23 |
| CN103945829B (en) | 2016-08-24 |
| JP2014177489A (en) | 2014-09-25 |
| JP6023756B2 (en) | 2016-11-09 |
| JPWO2013073431A1 (en) | 2015-04-02 |
| KR20130055495A (en) | 2013-05-28 |
| JP5584833B2 (en) | 2014-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103945829B (en) | Beauty method, protect skin composition, thalline and dry thalline | |
| CN102085159B (en) | Nursing type disinfecting liquid soap | |
| CN101730536B (en) | Anti-norovirus agent, and composition comprising the same | |
| US20210212318A1 (en) | Cleanser compositions and methods for using the same | |
| CN106413680A (en) | Niacinamide for inducing generation of antimicrobial peptides | |
| CN102639129A (en) | Antimicrobial compositions containing free fatty acids | |
| CN104257684B (en) | Milk cow nipple medicated bath plastics and preparation method thereof | |
| CN111093643A (en) | Antimicrobial compositions effective against bacteria and fungi | |
| CN103893022A (en) | Application of antibacterial peptide as preservative in preparing cosmetics | |
| CN108815141A (en) | Antibacterial peptide film and the preparation method and application thereof | |
| US10525017B2 (en) | Composition containing meso-2,3-butanediol | |
| JP5656375B2 (en) | Antibacterial agent and method for improving antibacterial property | |
| CN110251580A (en) | A kind of complex antimicrobials and its preparation method and application | |
| CN110339121A (en) | Bactericidal composition and the preparation method and application thereof | |
| Merchán et al. | An in vitro effectiveness evaluation of chemical agents for toothbrushes disinfection | |
| Shivappa et al. | Laboratory evaluation of safety and efficacy for Melafix (Melaleuca cajuputi extract) | |
| Mokhov et al. | Experimental Development of New Surgical Suturing Materials with Complex Biological Activities. | |
| CN111246871A (en) | Composition with inhibitory effect on virus and bacteria | |
| CN110157660A (en) | The induced medium and method, fibroblast and application of induced fibroblast secretion antibacterial peptide | |
| CN109806205A (en) | A kind of FRUCTUS TERMINALIAE IMMATURUS extract and its antibacterial application | |
| CN103767984A (en) | Hair-nourishing and bacteriostatic conditioning liquid shampoo | |
| CN105418759B (en) | A kind of anti-actinomyces naeslundii Yolk antibody and preparation method thereof | |
| CN103494742A (en) | Evening primrose oil antibacterial wipes and preparation method thereof | |
| CN114450019A (en) | Collagen production promoter, drug, cosmetic, and method for producing collagen production promoter | |
| CN108721167A (en) | A kind of antiseptic composition containing Ampelopsis grossedentata extrat and its purposes in preparing cosmetics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |