WO2013073431A1

WO2013073431A1 - Beauty treatment method, skin care composition, bacterial cell, and dried bacterial cell

Info

Publication number: WO2013073431A1
Application number: PCT/JP2012/078835
Authority: WO
Inventors: 英俊本多; 格出来尾
Original assignee: 株式会社バイオジェノミクス; 学校法人九州文化学園
Priority date: 2011-11-18
Filing date: 2012-11-07
Publication date: 2013-05-23
Also published as: CN103945829A; CN103945829B; JP5584833B2; JP6023756B2; JPWO2013073431A1; JP2014177489A; KR20130055495A; KR101406808B1

Abstract

Provided are: a novel and safe beauty treatment method which can cope with the individual variation of skin environments readily, has little adverse impacts such as adverse side effects, and enables the selective proliferation of a useful bacterium to thereby keep the normal bacterial skin flora healthy; a skin care composition; and a dried bacterium cell which can be used suitably for the production of the skin care composition. A beauty treatment method comprising a step of collecting bacteria inhabiting the surface of the skin of a human body, a step of selectively proliferating at least one useful bacterium that can be proliferated on the surface of the skin of a human body and can improve the health state of the skin among the collected bacteria, and a step of administering a composition containing at least one selected from the group consisting of a cell of the proliferated useful bacterium, a cell component of the useful bacterium and a substance produced by the useful bacterium onto the skin of a human body; and a skin care composition containing a useful bacterium that has been proliferated selectively in the above-mentioned manner and/or a substance produced by the useful bacterium.

Description

Cosmetic method, skin care composition, fungus body and dried fungus body

The present invention relates to a cosmetic method, a skin care composition that can be applied to all persons and has almost no side effects regardless of individual differences in skin environment, and a microbial cell and a dry microbial cell suitably used for the production thereof.

It is known that dozens of types of skin resident bacteria live on the surface of human skin. Some of the skin resident bacteria are harmful to the human body, such as Staphylococcus aureus, while other useful bacteria are useful for maintaining a healthy skin condition. Maintaining the condition is useful for improving the condition of the skin or skin surface. For example, Staphylococcus epidermidis contributes significantly to maintaining the skin's moisturizing function and barrier function by producing glycerin, and also produces organic acids and keeps the skin surface weakly acidic. Therefore, it plays a major role in suppressing the growth of harmful bacteria (see Non-Patent Document 1, for example). Furthermore, Staphylococcus epidermidis produces an antimicrobial peptide that inhibits the growth of Staphylococcus aureus, which is a harmful bacterium.

In order to prevent infectious diseases such as food poisoning and influenza and skin diseases such as acne, it is said that it is useful to keep the surface of the skin clean and suppress the growth of harmful bacteria. Therefore, detergents such as soap are used. However, if these detergents are used too much, the skin moisturizing components such as ceramide may be lost and useful bacteria may be killed, which may be counterproductive.

The existence ratio of each bacterial species in the resident skin flora is affected by various factors, but if the growth of useful bacteria is inhibited by any factor, harmful bacteria may become dominant and cause various diseases. . Therefore, research and development have been actively conducted on compounds that have a bactericidal action or a bacteriostatic action selectively only against harmful bacteria without affecting useful bacteria in order to improve the normal skin flora. For example, Patent Document 1 discloses that the balance of skin resident bacteria that works effectively only on the grown Acne bacteria (genus Propionibacterium) among the skin resident bacteria and does not act on aerobic bacteria is healthy. As a skin normal bacteria normalizing agent, what has an auren extract as an active ingredient is disclosed. Patent Document 2 discloses a skin resident flora-improving agent containing a water-soluble saccharide in which a plurality of sugars are β-bonded. Patent Document 3 discloses an ecosystem balance regulator for skin resident bacteria containing hinokitiol glycoside as an active ingredient for bacteriostasis and sterilizing Staphylococcus aureus and causing Staphylococcus epidermidis to grow. Patent Document 4 discloses one kind selected from a plant extract of the genus Kidney chicken and the genus Taconoki as an active ingredient having no bactericidal action against Staphylococcus epidermidis and having a bactericidal or growth-inhibiting action against Staphylococcus aureus. Or the ecosystem balance regulator of skin resident bacteria containing 2 or more types and bittern is disclosed.

JP 2010-202604 A JP 2008-50322 A JP 2007-246411 A JP 2007-153800 A

However, in the methods of Patent Documents 1 to 4 in which the skin resident flora is sounded by administering an exogenous compound, the drug resistance in harmful bacteria is acquired, the skin environment of each individual is different, etc. If the bacterial species constituting the skin resident flora are mutated and the drug susceptibility changes, there is a possibility that the soundening effect cannot be exhibited. It is extremely difficult to select an optimal drug according to such changes from a large number of candidate compounds in a custom-made manner.

The present invention has been made in view of the above problems, can easily cope with individual differences in the skin environment, has little adverse effects such as side effects, and healthy growth of the normal skin flora by selectively growing useful bacteria. It is an object of the present invention to provide a novel and safe cosmetic method, a skin care composition, and a dry cell body suitably used for the production thereof.

That is, the present invention provides a cosmetic method described in [1] to [7] below, a skin care composition described in [8] to [12], a fungus body described in [13] to [17], and a dried [18]. The solution of the above-described problem is achieved by providing the bacterial cells.
[1] A step of collecting bacteria that inhabit the human skin surface, and one or more useful bacteria that grow on the human skin surface and improve skin health among the bacteria are selectively grown. And a step of administering to the human skin a composition comprising one or more selected from the group consisting of cells of the proliferated useful bacteria, bacterial cell components, and substances produced by the useful bacteria .
[2] The cosmetic method according to the above [1], wherein in the administration step, the composition is administered (returned) to the same human individual from whom the bacteria have been collected.
[3] The cosmetic method according to [1] or [2], wherein the bacterium is a resident skin bacterium.
[4] The step of selectively growing the one or more kinds of useful bacteria comprises:
A pre-culturing step of culturing the collected bacteria on a medium;
A selection step of selecting the useful bacteria from the cultured bacteria;
The cosmetic method according to any one of [1] to [3], further comprising a culture step of culturing the selected useful bacteria.
[5] The cosmetic method according to any one of [1] to [4], wherein the bacteria live on the skin surface of a healthy human being.
[6] The cosmetic method according to any one of [1] to [5], wherein the useful bacteria are coagulase-negative staphylococci.
[7] The cosmetic method according to [6] above, wherein the useful bacterium is Staphylococcus epidermidis.
[8] One or a plurality of useful bacterial cells that are isolated from the skin resident bacteria collected from the human skin surface and selectively proliferated, grow on the human skin surface, and improve the health of the skin, A composition for skin care comprising one or more selected from the group consisting of bacterial cell components and substances produced by the useful bacteria.
[9] The skin care composition according to the above [8], wherein the bacteria are skin resident bacteria.
[10] The skin care composition according to the above [8] or [9], wherein the bacteria live on the skin surface of a healthy human state.
[11] The skin care composition according to any one of [8] to [10], wherein the useful bacteria are coagulase-negative staphylococci.
[12] The skin care composition according to the above [11], wherein the useful bacterium is Staphylococcus epidermidis.
[13] Among cells resident in the skin collected from the surface of human skin, cells obtained by selectively growing one or more useful bacteria that grow on the surface of human skin and improve the health of the skin .
[14] The bacterial cell according to [13] above, wherein the bacterium is a resident skin bacterium.
[15] The bacterial cell according to the above [13] or [14], wherein the bacterium lives on the skin surface of a healthy human state.
[16] The microbial cell according to any one of [13] to [15], wherein the useful bacterium is coagulase-negative staphylococci.
[17] The microbial cell according to [16] above, wherein the useful bacterium is Staphylococcus epidermidis.
[18] A dry cell obtained by drying the cell according to any one of [13] to [17].

Bacteria collected from human skin, in particular skin resident bacteria, are particularly prone to growth in human-specific skin environments. Therefore, a composition containing one or a plurality of useful bacteria selectively grown from bacteria collected from the surface of human skin, or a cell component or product of useful bacteria that promotes the growth is applied to the human skin surface. When administered, useful bacteria become dominant on the skin surface, so that the growth of harmful bacteria can be effectively suppressed, and thus the balance of the normal flora of the skin can be appropriately maintained. As a result, the health condition of the skin can be reliably maintained or improved. Therefore, the cosmetic method and the skin care composition provided by the present invention are useful for keeping the skin condition healthy. Further, the cosmetic method and the skin care composition of the present invention have the advantages that they are safe and have few adverse effects such as side effects because no drug is used.

In particular, bacteria collected from the skin of an individual, especially skin resident bacteria, are adapted to individual differences in the skin environment and are particularly prone to proliferate in the skin environment unique to that individual. For this reason, when bacteria that have been selectively grown from bacteria collected from the skin surface of the individual are administered (returned) to the skin surface of the individual, the useful bacteria are exposed to the skin surface regardless of individual differences in the skin environment. By being dominant, it is possible to more effectively suppress the growth of harmful bacteria, and thus to keep the balance of the normal skin flora more appropriately.

It is a graph which shows the influence which the returning operation | work of the dermal staphylococci derived from an own skin resident bacteria has on the number of Staphylococcus epidermidis on the skin surface. It is a graph which shows the influence which the return | restoration work of staphylococcus epidermidis derived from an own skin resident microbe has on the amount of epidermis moisture.

Hereinafter, an embodiment of the present invention (which is merely an example and does not limit the scope of the present invention) will be described.
A cosmetic method according to an embodiment of the present invention (hereinafter may be abbreviated as “cosmetic method”) includes a skin resident bacteria (bacteria of bacteria) that inhabit the surface of an individual (human individual). An example), a step of selectively growing one or more kinds of useful bacteria that grow on the skin surface of individuals and improve the health of the skin among the skin resident bacteria, Administering (returning) a skin care composition containing one or more selected from the group consisting of bacterial cells, bacterial cell components and useful bacterial product to the skin of an individual.

A composition for skin care used in a cosmetic method comprises one or a plurality of types of useful bacteria isolated from bacteria collected from the surface of an individual's skin and selectively proliferated, components of the cells, and products of useful bacteria Including one or more selected from the group and collecting bacteria living on the surface of the individual's skin, and selectively growing only one or more useful bacteria from the collected bacteria; It is manufactured by the method containing. Hereafter, each process regarding manufacture of the composition for skin care is demonstrated more concretely.

(1) Bacterial collection Bacteria such as dozens of skin resident bacteria are present on the surface of the skin, but as a bacterium used in the production of a skin care composition, the skin condition is healthy. Any one of these may be used alone, or any two or more of them may be used in appropriate combination as long as they are useful for maintenance. Examples of bacteria suitably used in the production of a skin care composition include human skin resident bacteria, and specific examples thereof include Staphylococcus epidermidis.

Various bacteria such as skin resident bacteria are present on the skin surface throughout the body, but are particularly abundant on the surface such as the face and armpits. Therefore, it is preferable to collect from these sites. In particular, when a skin care composition is used as a cosmetic, it is preferable to collect bacteria such as skin resident bacteria from the skin surface of the face. Since it is considered that the balance of skin resident bacteria is appropriately maintained on the healthy skin surface, it is preferable to collect the skin resident bacteria from the healthy (healthy) skin surface.

Bacteria can be collected using any method capable of collecting bacteria from the skin surface and isolating any strain. For example, a method of collecting by wiping the skin surface with any instrument such as a cotton swab, gauze, non-woven fabric, paper, sponge etc. A method of transferring bacteria from the skin surface to the surface of the culture medium by pressing onto the surface of the medium, a method of bringing a liquid such as water or physiological saline into contact with the skin surface, suspending the bacteria present on the skin surface in the liquid, and collecting it together with the liquid Etc. Since the bacterial species contained in the collected bacteria and the ratio of the numbers also serve as an index for diagnosing the health condition of the skin, it is preferable to collect the bacteria from a certain area on the skin. When collecting bacteria by wiping the skin surface with a sterile cotton swab, in order to keep the area wiped with a sterile cotton swab constant, it is made of a synthetic resin, glass, etc., open at both ends, cylindrical, elliptical cylinder, or prismatic Bacteria may be collected by lightly adhering the collection tool to the skin and wiping all over the opening. By such a method, bacteria can always be collected from the skin surface within a predetermined area range.

On the other hand, when bacteria are collected using a method in which a sterilized agar medium is pressed against the skin surface, the bacteria are always removed from the skin surface within a certain area by using a medium having a predetermined area (radius). Can be collected. The medium that can be used for collecting bacteria is not particularly limited, but specific examples of the medium used for collecting skin resident bacteria include standard agar medium, trypticase soy agar medium for normal living bacteria, egg yolk man Examples include selective separation media for staphylococci such as knit saline and salted egg agar. In addition, since the culture medium used for collection | recovery of bacteria is made to contact the skin surface, it is preferable to use the culture medium of the composition which does not contain the substance which may act as an allergen.

(2) Selective growth of useful bacteria The bacteria collected from the skin surface as described above include both useful bacteria and harmful bacteria. Of these, only one or more desired useful bacteria are selectively grown. As selective growth methods, there are a method in which only desired useful bacteria are isolated and cultured, and a method in which culture is performed under conditions in which only desired useful bacteria are grown, and either method may be used.

As an example of the former method, the step of selectively growing useful bacteria may include the following steps (i) to (iii).
(I) Preliminary culture process for culturing collected bacteria on medium (ii) Selection process for identifying cultured bacteria and selecting useful bacteria (iii) Culture process for culturing selected useful bacteria

(I) Pre-culture process Bacteria collected using a sterile cotton swab, etc. are diluted with a diluent such as phosphate buffered saline (PBS) at a predetermined magnification (eg, 10 to 100,000 times) and cultured. After applying to the surface of the culture medium, culture is performed. The culture conditions are appropriately set according to the conditions of the useful bacteria to be separated and the medium to be used.

In addition, when bacteria are collected by pressing the medium on the skin surface, the pre-culture process may be performed as it is on the surface of the collected medium, but it is re-collected from the medium surface using a sterile cotton swab etc. After the bacteria are diluted and applied to the surface of the culture medium, pre-culture may be performed using the same procedure as described above. Examples of the culture medium include a medium for general bacteria such as trypticase soy agar medium (TS medium), a selective separation medium for the aforementioned staphylococci, and the like.

(Ii) Selection process About the bacteria culture | cultivated at said preliminary culture process, a bacterial species is identified and a useful microbe is selected. Although a selection process can be performed using arbitrary well-known methods, it is performed in the following procedures, for example. First, the medium is visually observed, the number of colonies formed on the medium is counted, and, if possible, preliminary classification by appearance is performed. Then, bacterial species are identified for individual colonies or some representative of them. For example, in the case of Staphylococcus epidermidis, identification is performed by combining microscopic observation, Gram staining, and coagulase activity. In particular, Staphylococcus epidermidis is coagulase negative (CNS) unlike pathogenic bacteria such as Staphylococcus aureus. Therefore, measurement of coagulase activity is important for discrimination from Staphylococcus aureus.

(Iii) Culture process The useful bacteria selected (separated) from bacteria such as skin resident bacteria are cultured as described above. Culturing can be performed using any known method. The useful bacteria obtained in this way are considered to be suitable for the environment of the skin surface of the individual from whom the skin resident bacteria were collected. Therefore, they are contained in the skin care composition and administered (returned) to the skin surface of the individual. ), It proliferates predominantly on the skin surface, and can be expected to have effects such as improvement of the skin surface state and suppression of growth of harmful bacteria. About the culture medium and culture conditions used for culture | cultivation, it is suitably set according to conditions, such as the kind of useful microbe used as the objective of isolation | separation, and the culture medium to be used similarly to the case of a preculture process. In addition, when a plurality of useful bacteria are selected, those that are isolated for each strain and purely cultured may be mixed and used at a predetermined ratio at the time of manufacturing the skin care composition. You may culture in the state.

(3) Preparation of a composition for skin care In preparing a composition for skin care, live bacteria of useful bacteria selectively grown as described above may be used directly, but long-term storage is possible in a state containing the live bacteria. Because it is difficult, prepare asphyxia of useful bacteria or dry cells of useful bacteria, and restore the useful bacteria by dispersing in a base containing water just before use, for skin care containing useful bacteria A composition may be prepared.

The base material of the skin care composition is not particularly limited as long as useful bacteria can survive and / or grow therein, and any base such as lotion, milky lotion, serum, gel, cream, lotion, spray, etc. Special care products such as cosmetics and packs, hair tonics, hair lotions, hair growth agents, hair treatments, hair conditioners, hair packs, hair dyes and other scalp care products or hair cosmetics can be used. About the compounding quantity of the useful microbe per unit volume, it selects suitably according to a base material, the kind of useful microbe, etc.

Any known method can be used without particular limitation as a method for producing the dried cells, and specific examples thereof include a freeze drying method and a hot air drying method. For example, when producing dry cells by freeze-drying, useful bacteria are dispersed in a dispersion medium (usually water is used) in the presence of an appropriate protective agent such as skim milk or soy milk, and liquid nitrogen or the like is used. After freezing, remove water under reduced pressure with a freeze dryer. The dried cells thus obtained can be sealed and stored in a sterilized ampoule or the like.

In addition, when a cell component such as a dead cell of useful bacteria or a sonication product of a cell or a product produced by a useful cell has a specific growth promoting activity against the useful cell, the composition for skin care is a useful cell. These may be included instead of viable bacteria. Proportion of useful bacteria in the normal flora of skin by supplying a substance that specifically promotes the growth of useful bacteria on the skin surface of the individual instead of supplying live bacteria. This is because it is possible to increase the skin and keep the skin healthy. When using dead cells of useful bacteria or substances produced from useful bacteria instead of viable bacteria of useful bacteria, the skin care composition is a skin cleanser such as a shampoo, body shampoo, or head spa detergent containing a surfactant. It may be.

When the composition for skin care contains viable bacteria of useful bacteria, it may contain any additive usually used in cosmetics and the like as long as its production and / or growth is not inhibited. Specific examples of such additives include flavoring agents, preservatives, antioxidants, bactericides (limited to those having no or weak antibacterial activity against useful bacteria), emulsifiers, stabilizers, and the like. The composition for skin care may further contain a substance that specifically promotes the growth of useful bacteria.

By administering (returning) the skin care composition containing any one or more of the cells of the useful bacteria thus obtained, the bacterial cell components and the useful bacterial product to the individual's skin, (The site to be administered (returned) may be the same as or different from the site from which the skin resident bacteria were collected). Useful bacteria adapted to can grow rapidly on the skin surface and improve the health of the skin. In such a cosmetic method, the skin care composition can be administered in the form of a skin care composition such as application of liquids such as lotion, milky lotion, lotion, spraying of sprays, application of cream, gel, etc. Any method can be selected. Administration may be performed using fingers or the like, but may also be performed using a device such as a spray or an applicator. The administration interval and dosage per dose of the skin care composition can be adjusted as appropriate according to the type of useful bacteria, the site to be administered, the health condition of the skin, etc., but the bacteria are reliably fixed on the skin surface. In order to achieve this, it is preferable to administer continuously.

In the cosmetic method of the present embodiment, among bacteria collected from individual skin, only useful bacteria are selectively grown, and a skin care composition containing this is administered (returned) to the same individual. However, it may be administered to the skin surface of an individual different from the individual from whom the bacteria were collected. Or the composition for skin care containing the useful microbe selectively propagated from the bacteria extract | collected from several persons (The thing derived from the bacteria extract | collected from the person may be included) may be included. It may be administered.

Examples performed to confirm the effects of the present invention will be described below.
Example 1: Separation and pure culture of Staphylococcus epidermidis from resident skin bacterium and production of dry cells [1] Collection of resident skin bacterium The following operation was performed while wearing sterile gloves.
After washing, a polycarbonate cylinder (inner diameter 25 mmφ) sterilized in an autoclave is lightly pressed onto the skin surface of the subject's forehead, and the exposed skin surface inside the cylinder is sterilized phosphate buffered saline (PBS). Wipe with a sterile cotton swab soaked for 20 seconds. The cylinder was released and the moisture remaining on the skin surface was wiped off with a sterile cotton swab. The water adhering to the inside of the cylindrical skin contact surface was similarly wiped off with a sterile cotton swab. These sterilized cotton swabs were placed in a sterilized tube containing sterilized PBS (the part touched by the collector's hand was folded), capped and transferred into a safety cabinet.

[2] Preliminary culture 100 μL of the bacterial solution obtained as described above was added to a microtube containing 900 μL of PBS and vortexed to prepare a 10-fold diluted solution. The same operation was repeated using a diluted solution instead of the bacterial solution to prepare a 100 to 100,000-fold diluted solution. 50 μL of each of the obtained dilutions was added on trypticase soy agar medium (TS medium) and a selective medium for Staphylococcus aureus (hereinafter referred to as “selective medium”), and uniformly spread, and cultured at 37 ° C. for 48 hours. It was.

[3] Separation of Staphylococcus epidermidis Colonies formed on the TS medium and the selective medium were visually observed, and each colony was numbered. Each colony was purely cultured on a TS medium and a selective medium under the same culture conditions as in [2] above. Number each colony formed, place a drop of deionized water on the slide glass, disperse the cells collected from the colony using a platinum wire, and sprinkle it with a gas burner from the back. The body was fixed with flame. Crystal violet solution, iodine solution, and safranine solution were sequentially acted on this, and Gram staining was performed. Thereafter, the staining and morphology of the cells contained in each colony were observed with a microscope. At the same time, bacterial species were identified using an apistuff plate and confirmed to be Staphylococcus epidermidis.

[4] Pure culture of Staphylococcus epidermidis and production of dry cells [0104] Cells collected from colonies identified as Staphylococcus epidermidis were purely cultured on TS medium. From the obtained colonies, microbial cells were collected using a platinum loop (one colony having a diameter of 1 mm), dispersed in skim milk as a protective agent, poured into ampoules, and rapidly frozen in a -80 ° C ultra-low temperature chamber. This was subjected to a freeze dryer to obtain dry cell powder.

[5] Restoration of dried cells Ampoules containing dried cells were opened, and a small amount of liquid TS medium was added to suspend the dried cells. The obtained suspension was added to a liquid TS medium, a plate-like TS medium and a mannitol medium, and cultured at 37 ° C. for 48 hours. Were confirmed to form colonies. When these cells were identified, it was confirmed to be Staphylococcus epidermidis.

Example 2: Clinical study Using 21 females in their 20s to 50s as subjects, separation was performed from the skin resident bacteria collected from the skin (forehead) of the subject on the first day of the test using the procedure described in Example 1. In addition, the effect of administration (returning) of Staphylococcus epidermidis derived from the self-resident resident bacteria on purely cultured skin on the number of staphylococcus epidermidis on the skin surface and the water content of the epidermis was examined.

Twenty-one subjects were classified into a first group of 13 persons (indicated as “rejuvenating skin fungus return group” in FIGS. 1 and 2) and a second group of 8 persons (indicated as “placebo group” in FIGS. 1 and 2). (The subjects were not informed of which group was assigned.) Basic cosmetics (ethanol-free lotion (made by holin), moisturizing gel (made by holin), and milky lotion (made by holin)) were distributed. did. In addition, a dry cell of Staphylococcus epidermidis derived from the skin's own resident bacteria (hereinafter abbreviated as “dry cell”) was distributed to the first group, and a placebo (skimmed milk) was distributed to the second group. . For each group of subjects, apply lotion, dry cells (about 1 billion per gel use) or moisturizing gel with placebo suspended on face skin over 4 weeks, followed by emulsion. Therefore, the operation of administering (returning) Staphylococcus epidermidis derived from the self-resident skin bacterium or the placebo (referred to as “reverting beautiful skin bacteria” in FIG. 1; hereinafter, collectively referred to as “returning operation”). ) On a regular basis (1st and 4th day of each week). In order to examine the effect of the return operation, the number of Staphylococcus epidermidis on the skin surface of the forehead and the water content of the epidermis were measured periodically (once / week). The number of Staphylococcus epidermidis was measured by the method described in [1] and [2] of Example 1, and among the colonies of skin resident bacteria cultured after harvesting from the skin surface of the forehead, [3] of Example 1 This was done by counting the number of those identified as Staphylococcus epidermidis colonies by the method described. For the latter measurement, a cutometer MPA580 (skin viscoelasticity measuring device: manufactured by C + K, Germany) and a Corneometer probe were used (first course).

For 4 weeks after the end of the first course, only the subjects in the second group were subject to the same conditions as in the first course except that dry cells were distributed instead of placebo. We had you return work of staphylococci. In order to examine the effect of the return operation, the number of Staphylococcus epidermidis on the skin surface of the forehead was measured periodically (every two weeks). For the subjects of the first group, the number of Staphylococcus epidermidis on the skin surface of the forehead was measured when 4 weeks passed after the end of the first course (second course).

FIG. 1 shows the change over time in the number of Staphylococcus epidermidis (expressed as “relative number of skin-skins”) when the number of Staphylococcus epidermidis before the start of clinical trials in each group is 1. In the first group, the number of Staphylococcus epidermidis on the skin surface is significantly increased by administering (returning) Staphylococcus epidermidis derived from its own skin resident bacteria. When it stopped, it turned to decrease. On the other hand, in the second group, the number of Staphylococcus epidermidis on the skin surface is almost constant while the placebo is administered, whereas it is significant when switching to the returning operation of Staphylococcus epidermidis derived from its own skin resident bacteria. Increase was seen. These results are as follows: (1) staphylococcus epidermidis derived from its own skin resident bacteria is administered (returned), and staphylococcus epidermidis reliably settles on the skin surface. (2) Since the number of Staphylococcus epidermidis on the surface decreases, it is suggested that continuous administration (returning operation) is preferable.

FIG. 2 shows the amount of skin moisture (relative value of skin moisture) in the forehead before and after the end of the first cool in the first group and the second group. In the first group, it was confirmed that the amount of water in the epidermis was significantly increased by administering (returning) Staphylococcus epidermidis derived from the resident bacteria of the skin for 3 to 4 weeks in the first course. On the other hand, in the second group, it was observed that the water content of the epidermis was almost constant throughout the first course.

These results show that staphylococcus epidermidis derived from its own skin resident bacteria is administered (returned) to its own skin, so that staphylococcus epidermidis predominates in the resident skin flora, and accordingly, the health condition of the skin It strongly suggests that the moisture content of the epidermis, which is one index, has been improved.

Claims

Collecting bacteria that inhabit the human skin surface;
A step of selectively growing one or more useful bacteria that grow on the surface of human skin and improve skin health among the bacteria,
Administering to the human skin a composition containing one or more selected from the group consisting of the cells of the useful bacteria grown, the bacterial cell components, and the substances produced by the useful bacteria Beauty method.
The cosmetic method according to claim 1, wherein in the administering step, the composition is administered (returned) to the same human individual from whom the bacteria were collected.
The cosmetic method according to claim 1 or 2, wherein the bacteria are skin resident bacteria.
The step of selectively growing the one or more kinds of useful bacteria,
A pre-culturing step of culturing the collected bacteria on a medium;
A selection step of selecting the useful bacteria from the cultured bacteria;
The cosmetic method according to any one of claims 1 to 3, further comprising a culture step of culturing the selected useful bacteria.
The cosmetic method according to any one of claims 1 to 4, wherein the bacteria live on the skin surface of a healthy human being.
The cosmetic method according to any one of claims 1 to 5, wherein the useful bacteria are coagulase-negative staphylococci.
The cosmetic method according to claim 6, wherein the useful bacterium is Staphylococcus epidermidis.
One or a plurality of useful bacterial cells, bacterial components, and the above-mentioned useful substances that have been isolated and selectively grown from bacteria collected from the human skin surface, and that grow on the human skin surface and improve the health of the skin A composition for skin care, comprising one or more selected from the group consisting of fungal product.
The skin care composition according to claim 8, wherein the bacteria are skin resident bacteria.
10. The skin care composition according to claim 8 or 9, wherein the bacteria live on the skin surface of a healthy human state.
The skin care composition according to any one of claims 8 to 10, wherein the useful bacteria are coagulase-negative staphylococci.
12. The skin care composition according to claim 11, wherein the useful bacterium is Staphylococcus epidermidis.
A bacterial cell obtained by selectively proliferating one or more useful bacteria that grow on the human skin surface and improve the health of the skin among the bacteria collected from the human skin surface.
The bacterium according to claim 13, wherein the bacterium is a resident skin bacterium.
15. The microbial cell according to claim 13 or 14, wherein the bacterium lives on the skin surface of a healthy human state.
The microbial cell according to any one of claims 13 to 15, wherein the useful bacterium is a coagulase-negative staphylococci.
The microbial cell according to claim 16, wherein the useful bacterium is Staphylococcus epidermidis.
A dried microbial cell obtained by drying the microbial cell according to any one of claims 13 to 17.