CN103937174B - One has germ resistance and bioactive biodegradable matrix material and preparation method thereof - Google Patents
One has germ resistance and bioactive biodegradable matrix material and preparation method thereof Download PDFInfo
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Abstract
The invention discloses one and there is germ resistance and bioactive biodegradable matrix material and preparation method thereof.This matrix material comprises biodegradable matrices and is scattered in germ resistance biologically active substance wherein, and germ resistance biologically active substance is obtained by Streptomyces jingyangensis fermentation.The inventive method comprises the preparation of germ resistance and biologically active substance, has the preparation of germ resistance and bioactive biodegradable matrix material.The method has that the cycle is short, cost is low, technique is simple, be convenient to the advantages such as suitability for industrialized production, and stability and circulation ratio are all better.The anti-microbial property of the matrix material that the present invention obtains is good, and antibacterium rate can reach more than 91%, antifungal ability reaches more than 1 grade; Degradation property is good, places degradation rate after 6 months and all can reach more than 90%, have and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, and do not harm environment after degraded in compost, active sludge, soil.
Description
Technical field
The present invention relates to a kind of biodegradable matrix material, be specifically related to one and there is germ resistance and bioactive biodegradable matrix material and preparation method thereof.
Background technology
Along with carrying out in a deep going way of scientific research and improving constantly of people's living standard, matrix material is subject to people's attention day by day.Wherein, functional composite material can meet people on the basis of the basic demand of material because of it, has various specific function concurrently and has a good application prospect.The simultaneously resource exhaustion caused by development of global economy and ecological environment problem are subject to people's attention day by day, and development renewable resources, alleviates the important directions that ecological environmental pollution is current scientific research and practical application.The development of functional degradation material and use are the important channels in this direction of development.At present, the country such as Japan and America and Europe has dropped into the research that a large amount of man power and materials carries out functional degradation material, and has defined technology and the product of comparative maturity.China is still in test and exploratory stage for the research of this series products, and the functional degradation material of development low cost is the emphasis direction of the current matrix material development of China.
Summary of the invention
The object of the present invention is to provide one to have germ resistance and bioactive biodegradable matrix material and preparation method thereof, this matrix material is nontoxic, is easy to degraded, reproducible utilization, environmental protection after discarded; The method cycle is short, cost is low, technique is simple, stability and circulation ratio are all better, is convenient to industrialization raw.
The present invention is achieved through the following technical solutions:
A kind of preparation method with germ resistance and bioactive biodegradable matrix material, biodegradable matrices is heated to complete melting, then germ resistance biologically active substance, dispersion agent and coupling agent is mixed, mixing 5 ~ 20min at 60 ~ 180 DEG C, naturally cooling, obtained have germ resistance and bioactive biodegradable matrix material; Wherein, the quality of the germ resistance biologically active substance, dispersion agent and the coupling agent that add is respectively biodegradable matrices quality 1 ~ 10%, 0.01 ~ 10% and 0.01 ~ 10%.
Described biodegradable matrices is PBS base co-polymer, PLA base co-polymer or PCL base co-polymer.
Described germ resistance biologically active substance is obtained by Streptomyces jingyangensis fermentation, and concrete preparation comprises the following steps:
1) Streptomyces jingyangensis is activated, obtain the Streptomyces jingyangensis activated, by the Streptomyces jingyangensis enlarged culturing of activation, obtain Streptomyces jingyangensis seed liquor, by Streptomyces jingyangensis seed liquor fermentation culture, obtain Streptomyces jingyangensis fermented liquid;
2) Streptomyces jingyangensis fermented liquid is got, centrifugal, obtain fermentation clear liquid and precipitation mycelia; By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Mycelia 80 ~ 100% alcohol steep 20 ~ 24h will be precipitated, centrifugal, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance biologically active substance.
Activation described in step 1) is inoculated in activation medium by Streptomyces jingyangensis, at 28 ~ 32 DEG C, once cultivate 40 ~ 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification be again inoculated in activation medium, at 28 ~ 32 DEG C, second incubation 40 ~ 50h, obtains the Streptomyces jingyangensis activated;
Wherein, described activation medium comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, agar 15 ~ 20g/L, pH value is 6.5 ~ 7.5.
Enlarged culturing described in step 1) is inoculated in by the Streptomyces jingyangensis of activation to spread cultivation in substratum, and be 28 ~ 32 DEG C in temperature, rotating speed is under the condition of 150 ~ 250r/min, and shaking culture 30 ~ 40h obtains Streptomyces jingyangensis seed liquor;
Wherein, the described substratum that spreads cultivation comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, pH value is 6.5 ~ 7.5.
Fermentation culture described in step 1) is inoculated in fermention medium according to the inoculum size of 100mL culture medium inoculated 3 ~ 6mL seed liquor by Streptomyces jingyangensis seed liquor, and at 100 ~ 150r/min, at 25 ~ 30 DEG C, fermentation 4 ~ 7d, obtains Streptomyces jingyangensis fermented liquid;
Wherein, described fermention medium comprises soybean cake powder 15 ~ 20g/L, glucose 15 ~ 20g/L, sodium-chlor 2.5 ~ 3.5g/L, calcium carbonate 0.5 ~ 1.5g/L, and pH value is 6.5 ~ 7.5.
Step 2) described in centrifugal be under rotating speed is the condition of 3000 ~ 4000r/min, centrifugal 15 ~ 25min.
Described dispersion agent is low-molecular-weight wax, ionic polymer, stearates or polymer hyper-dispersant.
Described coupling agent is silane coupling agent, titanate coupling agent, zirconium ester coupling agent, aluminate coupling agent or Aluminum zirconium coupling agent.
One has germ resistance and bioactive biodegradable matrix material, and the antibacterium rate of this matrix material reaches more than 91%, antifungal ability reaches more than 1 grade, and degradation rate reaches more than 90%.
Compared with prior art, the present invention has following useful technique effect:
1, the present invention utilizes Streptomyces jingyangensis meta-bolites to have germ resistance and bioactive feature, Streptomyces jingyangensis is fermented, preparation germ resistance biologically active substance, carry out mixing with the matrix with degradability, obtained have germ resistance and bioactive biodegradable matrix material, this matrix material all has good antimicrobial property to ten kinds of common crops pathomycetes and the streptococcus aureuses etc. such as cotton-wilt fusarium four kinds of common causative bacteriums, antibacterium rate can reach more than 91%, antifungal ability reaches more than 1 grade, and anti-microbial property is good.There is after simultaneously contained in this matrix material biologically active substance makes it degrade the effect of short plant germination and growth, fixed nitrogen, potassium decomposing, phosphorus decomposing, therefore this matrix material is that one has good germ resistance and bioactive material, can be widely used in the material such as mulch film, food casing.
What 2, the present invention obtained has in germ resistance and bioactive biodegradable matrix material, use antibacterial and biologically active substance to be fermentable gained, a kind of to environment and the harmless biomaterial of human non-toxic, thus utilize its matrix material prepared to environment and human non-toxic harmless.This antibacterial and biologically active substance is prepared by microorganism large scale fermentation, and low production cost, its utilization can reduce the production cost of matrix material.
What 3, the present invention obtained has in germ resistance and bioactive biodegradable matrix material, use matrix for degradability matrix, degraded is easy to after matrix material goes out of use, do not harm environment, in compost, active sludge, soil, place degradation rate after 6 months all can reach more than 90%, be a kind of environmental protection, renewable resources, and there is after degraded biological activity such as promotion plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing etc., do not harm environment.
What 4, the present invention obtained has germ resistance and bioactive biodegradable matrix material, its production does not rely on the Mineral resources such as oil, Sweet natural gas, abundance, energy-conserving and environment-protective and relative low price, stable, be the favorable substitutes of existing plastic prod.
5, the inventive method have that the cycle is short, cost is low, technique is simple, low for equipment requirements, stability and high repeatability and other advantages, be applicable to industrialization scale operation.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
The present invention mainly comprises the following steps:
The first step: preparation germ resistance biologically active substance;
Second step: the germ resistance biologically active substance the first step prepared and the master batch of biodegradable matrices are put into Banbury mixer and carried out mixingly having germ resistance and bioactive biodegradable matrix material with obtained the present invention.
The preparation method of germ resistance and biologically active substance in the first step, comprises the following steps:
1) Streptomyces jingyangensis is activated, obtain the Streptomyces jingyangensis activated, by the Streptomyces jingyangensis enlarged culturing of activation, obtain Streptomyces jingyangensis seed liquor, by Streptomyces jingyangensis seed liquor fermentation culture, obtain Streptomyces jingyangensis fermented liquid;
Activation is inoculated in activation medium by Streptomyces jingyangensis, at 28 ~ 32 DEG C, once cultivate 40 ~ 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification be again inoculated in activation medium, at 28 ~ 32 DEG C, second incubation 40 ~ 50h, obtains the Streptomyces jingyangensis activated; Wherein, activation medium comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, agar 15 ~ 20g/L, pH value is 6.5 ~ 7.5; (namely in every 1000mL tap water, comprise 15 ~ 20g Zulkovsky starch, 0.5 ~ 1.0g saltpetre, 0.2 ~ 0.6g potassium primary phosphate, 0.2 ~ 0.6g sodium-chlor, 0.005 ~ 0.015g ferrous sulfate, 0.2 ~ 0.6g magnesium sulfate and 15 ~ 20g agar, pH value is 6.5 ~ 7.5);
Enlarged culturing is inoculated in by the Streptomyces jingyangensis of activation to spread cultivation in substratum, and be 28 ~ 32 DEG C in temperature, rotating speed is under the condition of 150 ~ 250r/min, and shaking culture 30 ~ 40h obtains Streptomyces jingyangensis seed liquor; Wherein, the substratum that spreads cultivation comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, and pH value is 6.5 ~ 7.5; (namely in every 1000mL tap water, comprise 15 ~ 20g Zulkovsky starch, 0.5 ~ 1.0g saltpetre, 0.2 ~ 0.6g potassium primary phosphate, 0.2 ~ 0.6g sodium-chlor, 0.005 ~ 0.015g ferrous sulfate and 0.2 ~ 0.6g magnesium sulfate, pH value is 6.5 ~ 7.5);
Fermentation culture is inoculated in fermention medium according to the inoculum size of 100mL culture medium inoculated 3 ~ 6mL seed liquor by Streptomyces jingyangensis seed liquor, and at 100 ~ 150r/min, at 25 ~ 30 DEG C, fermentation 4 ~ 7d, obtains Streptomyces jingyangensis fermented liquid; Wherein, described fermention medium comprises soybean cake powder 15 ~ 20g/L, glucose 15 ~ 20g/L, sodium-chlor 2.5 ~ 3.5g/L, calcium carbonate 0.5 ~ 1.5g/L, and pH value is 6.5 ~ 7.5; (namely in every 1000mL tap water, comprise 15 ~ 20g soybean cake powder, 15 ~ 20g glucose, 2.5 ~ 3.5g sodium-chlor and 0.5 ~ 1.5g calcium carbonate, pH value is 6.5 ~ 7.5);
2) getting Streptomyces jingyangensis fermented liquid, is under the condition of 3000 ~ 4000r/min at rotating speed, centrifugal 15 ~ 25min, obtains fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, with 80 ~ 100% alcohol steep 20 ~ 24h, vat liquor is under rotating speed is the condition of 3000 ~ 4000r/min, centrifugal 15 ~ 25min, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtains secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance biologically active substance.
The mixing step of second step is: the master batch of biodegradable matrices is put into Banbury mixer and heated, after complete melting, mix outward mass percent be 1 ~ 10% germ resistance biologically active substance, mass percent be 0.01 ~ 10% dispersion agent and mass percent be the coupling agent of 0.01 ~ 10%, at 60 ~ 180 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
Biodegradable matrix in the present invention is PBS base co-polymer, PLA base co-polymer or PCL base co-polymer mainly.
The dispersion agent added time mixing can be: low one's share of expenses for a joint undertaking wax, ionic polymer, stearates or polymer hyper-dispersant.
Dispersion agent is specially: EVA-3, CH-2C, 295A, Zinic stearas, LuwaxAF-29, K-2, N-34 or stearic acid;
The coupling agent added time mixing can be: silane coupling agent, titanate coupling agent, zirconium ester coupling agent, aluminate coupling agent or Aluminum zirconium coupling agent.
Coupling agent is specially: KH-550, TTOPP-38S, ModA, DL-411-A, Z-6020 or TL-2B.
Below in conjunction with specific embodiment, the present invention is described in further detail.
Bacterial classification described in the embodiment of the present invention: Streptomyces jingyangensis (Streptomycesjingyangensis), purchased from Guangdong Province's Culture Collection, bacterial strain GIM4.92.
Embodiment 1
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, agar 15g/L, and pH value is 6.5.Inoculation Streptomyces jingyangensis, in activation medium, at 28 DEG C, once cultivates 40h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 28 DEG C, second incubation 40h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, and pH value is 6.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 28 DEG C in temperature, and rotating speed is under the condition of 150r/min, and shaking culture 30h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 15g/L, glucose 15g/L, sodium-chlor 2.5g/L, calcium carbonate 0.5g/L, and pH value is 6.5.According to the inoculum size of every 100mL culture medium inoculated 3mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 100r/min, at 25 DEG C, fermentation 4d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 15min under 3000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 80% alcohol steep 20h is added according to the solid-liquid ratio of 1g:1mL, centrifugal 15min under 3000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PBS base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PBS base co-polymer master batch, mix outward mass percent be 1% germ resistance and biologically active substance, mass percent be 0.15% dispersion agent EVA-3 and mass percent be 0.15% coupling agent KH-550, at 110 ~ 130 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 91%, antifungal ability reaches more than 1 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 92%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 8%, plant-growth rate can improve 4%, plant respiration intensity can improve 20%, nitrogen content can be utilized in soil to improve 15%, potassium content can be utilized in soil to improve 70%, phosphorus content in soil, can be utilized to improve 80%.
Embodiment 2
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, agar 18g/L, and pH value is 7.0.Inoculation Streptomyces jingyangensis, in activation medium, at 30 DEG C, once cultivates 45h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 30 DEG C, second incubation 45h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, and pH value is 7.0.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 30 DEG C in temperature, and rotating speed is under the condition of 200r/min, and shaking culture 35h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 18g/L, glucose 18g/L, sodium-chlor 3.0g/L, calcium carbonate 1.0g/L, and pH value is 7.0.According to the inoculum size of every 100mL culture medium inoculated 5mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 120r/min, at 28 DEG C, fermentation 5d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 18min under 3500r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 90% alcohol steep 22h is added according to the solid-liquid ratio of 1.5g:1mL, centrifugal 20min under 3500r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PBS base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PBS base co-polymer master batch, mix outward mass percent be 5% germ resistance and biologically active substance, mass percent be 10% dispersing agent C H-2C and mass percent be 0.3% coupling agent TTOPP-38S, at 110 ~ 130 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 95%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 93%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 15%, plant-growth rate can improve 10%, plant respiration intensity can improve 35%, nitrogen content can be utilized in soil to improve 25%, potassium content can be utilized in soil to improve 140%, phosphorus content in soil, can be utilized to improve 150%.
Embodiment 3
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, agar 20g/L, and pH value is 7.5.Inoculation Streptomyces jingyangensis, in activation medium, at 32 DEG C, once cultivates 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 32 DEG C, second incubation 50h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, and pH value is 7.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 32 DEG C in temperature, and rotating speed is under the condition of 250r/min, and shaking culture 40h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 20g/L, glucose 20g/L, sodium-chlor 3.5g/L, calcium carbonate 1.5g/L, and pH value is 7.5.According to the inoculum size of every 100mL culture medium inoculated 6mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 150r/min, at 25 ~ 30 DEG C, fermentation 7d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 25min under 4000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 100% alcohol steep 24h is added according to the solid-liquid ratio of 2g:1mL, centrifugal 25min under 4000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PBS base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PBS base co-polymer master batch, mix outward mass percent be 10% germ resistance and biologically active substance, mass percent be 8% dispersion agent 295A and mass percent be 4.2% coupling agent ModA, at 110 ~ 130 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 99%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 92%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 20%, plant-growth rate can improve 15%, plant respiration intensity can improve 40%, nitrogen content can be utilized in soil to improve 35%, potassium content can be utilized in soil to improve 200%, phosphorus content in soil, can be utilized to improve 250%.
Embodiment 4
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, agar 15g/L, and pH value is 6.5.Inoculation Streptomyces jingyangensis, in activation medium, at 28 DEG C, once cultivates 40h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 28 DEG C, second incubation 40h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, and pH value is 6.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 28 DEG C in temperature, and rotating speed is under the condition of 150r/min, and shaking culture 30h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 15g/L, glucose 15g/L, sodium-chlor 2.5g/L, calcium carbonate 0.5g/L, and pH value is 6.5.According to the inoculum size of every 100mL culture medium inoculated 3mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 100r/min, at 25 DEG C, fermentation 4d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 15min under 3000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 80% alcohol steep 20h is added according to the solid-liquid ratio of 1g:1mL, centrifugal 15min under 3000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PLA base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PLA base co-polymer master batch, mix germ resistance and biologically active substance, the mass percent dispersion agent Zinic stearas that is 0.01%, the mass percent coupling agent DL-411-A that is 4% that mass percent is 1% outward, at 160 ~ 180 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 90%, antifungal ability reaches more than 1 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 95%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 9%, plant-growth rate can improve 5%, plant respiration intensity can improve 23%, nitrogen content can be utilized in soil to improve 16%, potassium content can be utilized in soil to improve 75%, phosphorus content in soil, can be utilized to improve 82%.
Embodiment 5
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, agar 18g/L, and pH value is 7.0.Inoculation Streptomyces jingyangensis, in activation medium, at 30 DEG C, once cultivates 45h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 30 DEG C, second incubation 45h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, and pH value is 7.0.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 30 DEG C in temperature, and rotating speed is under the condition of 200r/min, and shaking culture 35h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 18g/L, glucose 18g/L, sodium-chlor 3.0g/L, calcium carbonate 1.0g/L, and pH value is 7.0.According to the inoculum size of every 100mL culture medium inoculated 5mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 120r/min, at 28 DEG C, fermentation 5d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 18min under 3500r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 90% alcohol steep 22h is added according to the solid-liquid ratio of 1.5g:1mL, centrifugal 20min under 3500r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PLA base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PLA base co-polymer master batch, mix outward mass percent be 5% germ resistance and biologically active substance, mass percent be 8% dispersion agent 295A and mass percent be 10% coupling agent Z-6020, at 160 ~ 180 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 95%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 95%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 18%, plant-growth rate can improve 13%, plant respiration intensity can improve 37%, nitrogen content can be utilized in soil to improve 26%, potassium content can be utilized in soil to improve 150%, phosphorus content in soil, can be utilized to improve 160%.
Embodiment 6
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, agar 20g/L, and pH value is 7.5.Inoculation Streptomyces jingyangensis, in activation medium, at 32 DEG C, once cultivates 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 32 DEG C, second incubation 50h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, and pH value is 7.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 32 DEG C in temperature, and rotating speed is under the condition of 250r/min, and shaking culture 40h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 20g/L, glucose 20g/L, sodium-chlor 3.5g/L, calcium carbonate 1.5g/L, and pH value is 7.5.According to the inoculum size of every 100mL culture medium inoculated 6mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 150r/min, at 25 ~ 30 DEG C, fermentation 7d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 25min under 4000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 100% alcohol steep 24h is added according to the solid-liquid ratio of 2g:1mL, centrifugal 25min under 4000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PLA base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PLA base co-polymer master batch, mix outward mass percent be 10% germ resistance and biologically active substance, mass percent be 8% dispersion agent LuwaxAF-29 and mass percent be 10% coupling agent Z-6020, at 160 ~ 180 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 99%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 95%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 23%, plant-growth rate can improve 17%, plant respiration intensity can improve 45%, nitrogen content can be utilized in soil to improve 38%, potassium content can be utilized in soil to improve 220%, phosphorus content in soil, can be utilized to improve 270%.
Embodiment 7
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, agar 15g/L, and pH value is 6.5.Inoculation Streptomyces jingyangensis, in activation medium, at 28 DEG C, once cultivates 40h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 28 DEG C, second incubation 40h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 15g/L, saltpetre 0.5g/L, potassium primary phosphate 0.2g/L, sodium-chlor 0.2g/L, ferrous sulfate 0.005g/L, magnesium sulfate 0.2g/L, and pH value is 6.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 28 DEG C in temperature, and rotating speed is under the condition of 150r/min, and shaking culture 30h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 15g/L, glucose 15g/L, sodium-chlor 2.5g/L, calcium carbonate 0.5g/L, and pH value is 6.5.According to the inoculum size of every 100mL culture medium inoculated 3mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 100r/min, at 25 DEG C, fermentation 4d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 15min under 3000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 80% alcohol steep 20h is added according to the solid-liquid ratio of 1g:1mL, centrifugal 15min under 3000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PCL base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PCL base co-polymer master batch, mix outward mass percent be 1% germ resistance and biologically active substance, mass percent be 3.1% dispersion agent K-2 and mass percent be 1.5% coupling agent TL-2B, at 60 ~ 80 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 90%, antifungal ability reaches more than 1 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 92%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 8%, plant-growth rate can improve 6%, plant respiration intensity can improve 22%, nitrogen content can be utilized in soil to improve 15%, potassium content can be utilized in soil to improve 70%, phosphorus content in soil, can be utilized to improve 81%.
Embodiment 8
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, agar 18g/L, and pH value is 7.0.Inoculation Streptomyces jingyangensis, in activation medium, at 30 DEG C, once cultivates 45h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 30 DEG C, second incubation 45h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 18g/L, saltpetre 0.8g/L, potassium primary phosphate 0.4g/L, sodium-chlor 0.4g/L, ferrous sulfate 0.01g/L, magnesium sulfate 0.4g/L, and pH value is 7.0.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 30 DEG C in temperature, and rotating speed is under the condition of 200r/min, and shaking culture 35h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 18g/L, glucose 18g/L, sodium-chlor 3.0g/L, calcium carbonate 1.0g/L, and pH value is 7.0.According to the inoculum size of every 100mL culture medium inoculated 5mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 120r/min, at 28 DEG C, fermentation 5d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 18min under 3500r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 90% alcohol steep 22h is added according to the solid-liquid ratio of 1.5g:1mL, centrifugal 20min under 3500r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PCL base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PCL base co-polymer master batch, mix outward mass percent be 5% germ resistance and biologically active substance, mass percent be 2.1% dispersion agent N-34 and mass percent be 3.4% coupling agent KM-602, at 60 ~ 80 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 95%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 92%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 16%, plant-growth rate can improve 11%, plant respiration intensity can improve 34%, nitrogen content can be utilized in soil to improve 25%, potassium content can be utilized in soil to improve 140%, phosphorus content in soil, can be utilized to improve 150%.
Embodiment 9
There is a preparation method for germ resistance and bioactive biodegradable matrix material, comprise the following steps:
(1) activation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis activation medium, activation medium comprises starch soluble 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, agar 20g/L, and pH value is 7.5.Inoculation Streptomyces jingyangensis, in activation medium, at 32 DEG C, once cultivates 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification and is again inoculated in activation medium, at 32 DEG C, second incubation 50h, obtains the Streptomyces jingyangensis activated.
(2) the spreading cultivation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis to spread cultivation substratum, the substratum that spreads cultivation comprises Zulkovsky starch 20g/L, saltpetre 1.0g/L, potassium primary phosphate 0.6g/L, sodium-chlor 0.6g/L, ferrous sulfate 0.015g/L, magnesium sulfate 0.6g/L, and pH value is 7.5.Being inoculated in by the Streptomyces jingyangensis of activation spreads cultivation in substratum, is 32 DEG C in temperature, and rotating speed is under the condition of 250r/min, and shaking culture 40h obtains Streptomyces jingyangensis seed liquor.
(3) fermentation of Streptomyces jingyangensis
Prepare Streptomyces jingyangensis fermention medium, fermention medium comprises soybean cake powder 20g/L, glucose 20g/L, sodium-chlor 3.5g/L, calcium carbonate 1.5g/L, and pH value is 7.5.According to the inoculum size of every 100mL culture medium inoculated 6mL seed liquor, be inoculated in fermention medium by Streptomyces jingyangensis seed liquor, at 150r/min, at 25 ~ 30 DEG C, fermentation 7d, obtains Streptomyces jingyangensis fermented liquid.
(4) germ resistance and bioactive preparation
Get Streptomyces jingyangensis fermented liquid, centrifugal 25min under 4000r/min condition, obtain fermentation clear liquid and precipitation mycelia.By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Get precipitation mycelia, in precipitation mycelia, 100% alcohol steep 24h is added according to the solid-liquid ratio of 2g:1mL, centrifugal 25min under 4000r/min condition, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance and biologically active substance.
(5) mixing
The master batch of biodegradable matrix PCL base co-polymer is put into Banbury mixer heat, after the complete melting of above-mentioned PCL base co-polymer master batch, mix outward mass percent be 10% germ resistance and biologically active substance, mass percent be 3.5% dispersion agent stearic acid and mass percent be 8% coupling agent Zr-801, at 60 ~ 80 DEG C of mixing 5 ~ 20min, take off after naturally cooling, obtained the present invention has germ resistance and bioactive biodegradable matrix material.
The effect of the present embodiment is: obtained have that germ resistance and bioactive biodegradable matrix material antibacterium rate can reach 99%, antifungal ability reaches more than 0 grade, and anti-microbial property is good; In compost, active sludge, soil, place degradation rate after 6 months all can reach 91%; Have after degraded and promote the biological activity such as plant germination and growth and fixed nitrogen potassium decomposing phosphorus decomposing, plant germination rate can improve 21%, plant-growth rate can improve 16%, plant respiration intensity can improve 41%, nitrogen content can be utilized in soil to improve 33%, potassium content can be utilized in soil to improve 210%, phosphorus content in soil, can be utilized to improve 260%.
In sum, Streptomyces jingyangensis meta-bolites prepared by the present invention has stronger anti-microbial property, simultaneously wherein containing large number of biological active substance, has and promotes the effect such as plant germination and growth, fixed nitrogen potassium decomposing phosphorus decomposing.Streptomyces jingyangensis meta-bolites is utilized to prepare germ resistance and biologically active substance, prepare with degradability matrix composite and there is germ resistance and bioactive degradability matrix material, it is made to be applied to agricultural mulching, wrapping material, the various products such as food product containers, lotion container, seasickness bag, stationery, leisure goods, body of a motor car inner and outer decorative parts, to the suitability for industrialized production of promotion functions degradable composite material and widespread use significant.
Claims (8)
1. one kind has the preparation method of germ resistance and bioactive biodegradable matrix material, it is characterized in that, biodegradable matrices is heated to complete melting, then germ resistance biologically active substance, dispersion agent and coupling agent is mixed, mixing 5 ~ 20min at 60 ~ 180 DEG C, naturally cooling, obtained have germ resistance and bioactive biodegradable matrix material; Wherein, the quality of the germ resistance biologically active substance, dispersion agent and the coupling agent that add is respectively biodegradable matrices quality 1 ~ 10%, 0.01 ~ 10% and 0.01 ~ 10%;
Described biodegradable matrices is PBS base co-polymer, PLA base co-polymer or PCL base co-polymer;
Described germ resistance biologically active substance is obtained by Streptomyces jingyangensis fermentation, and concrete preparation comprises the following steps:
1) Streptomyces jingyangensis is activated, obtain the Streptomyces jingyangensis activated, by the Streptomyces jingyangensis enlarged culturing of activation, obtain Streptomyces jingyangensis seed liquor, by Streptomyces jingyangensis seed liquor fermentation culture, obtain Streptomyces jingyangensis fermented liquid;
2) Streptomyces jingyangensis fermented liquid is got, centrifugal, obtain fermentation clear liquid and precipitation mycelia; By fermentation clear liquid lyophilize, obtain one-level germ resistance biologically active substance; Mycelia 80 ~ 100% alcohol steep 20 ~ 24h will be precipitated, centrifugal, supernatant liquor is mycelia vat liquor, by the lyophilize of mycelia vat liquor, obtain secondary germ resistance biologically active substance, one-level germ resistance biologically active substance is mixed with secondary germ resistance biologically active substance, obtains germ resistance biologically active substance.
2. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, it is characterized in that, step 1) described in activation be that Streptomyces jingyangensis is inoculated in activation medium, at 28 ~ 32 DEG C, once cultivate 40 ~ 50h, obtain Streptomyces jingyangensis and once cultivate bacterial classification, this Streptomyces jingyangensis is once cultivated bacterial classification be again inoculated in activation medium, at 28 ~ 32 DEG C, second incubation 40 ~ 50h, obtains the Streptomyces jingyangensis activated;
Wherein, described activation medium comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, agar 15 ~ 20g/L, pH value is 6.5 ~ 7.5.
3. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, it is characterized in that, step 1) described in enlarged culturing be the Streptomyces jingyangensis of activation is inoculated in spread cultivation in substratum, it is 28 ~ 32 DEG C in temperature, rotating speed is under the condition of 150 ~ 250r/min, shaking culture 30 ~ 40h, obtains Streptomyces jingyangensis seed liquor;
Wherein, the described substratum that spreads cultivation comprises Zulkovsky starch 15 ~ 20g/L, saltpetre 0.5 ~ 1.0g/L, potassium primary phosphate 0.2 ~ 0.6g/L, sodium-chlor 0.2 ~ 0.6g/L, ferrous sulfate 0.005 ~ 0.015g/L, magnesium sulfate 0.2 ~ 0.6g/L, pH value is 6.5 ~ 7.5.
4. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, it is characterized in that, step 1) described in fermentation culture, according to the inoculum size of 100mL culture medium inoculated 3 ~ 6mL seed liquor, Streptomyces jingyangensis seed liquor is inoculated in fermention medium, at 100 ~ 150r/min, at 25 ~ 30 DEG C, fermentation 4 ~ 7d, obtains Streptomyces jingyangensis fermented liquid;
Wherein, described fermention medium comprises soybean cake powder 15 ~ 20g/L, glucose 15 ~ 20g/L, sodium-chlor 2.5 ~ 3.5g/L, calcium carbonate 0.5 ~ 1.5g/L, and pH value is 6.5 ~ 7.5.
5. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, is characterized in that, step 2) described in centrifugal be under rotating speed is the condition of 3000 ~ 4000r/min, centrifugal 15 ~ 25min.
6. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, is characterized in that, described dispersion agent is low-molecular-weight wax, ionic polymer, stearates or polymer hyper-dispersant.
7. a kind of preparation method with germ resistance and bioactive biodegradable matrix material according to claim 1, it is characterized in that, described coupling agent is silane coupling agent, titanate coupling agent, zirconium ester coupling agent, aluminate coupling agent or Aluminum zirconium coupling agent.
8. one kind adopt the method in claim 1 ~ 7 described in any one to prepare there is germ resistance and bioactive biodegradable matrix material, it is characterized in that, the antibacterium rate of this matrix material reaches more than 91%, antifungal ability reaches more than 1 grade, and degradation rate reaches more than 90%.
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