CN103919056A - Method for improving activity of glutamic acid decarboxylase (GAD) of immature brown rice grains - Google Patents
Method for improving activity of glutamic acid decarboxylase (GAD) of immature brown rice grains Download PDFInfo
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- CN103919056A CN103919056A CN201410163372.6A CN201410163372A CN103919056A CN 103919056 A CN103919056 A CN 103919056A CN 201410163372 A CN201410163372 A CN 201410163372A CN 103919056 A CN103919056 A CN 103919056A
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- brown rice
- immature
- immature kernel
- rice
- gad
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- 235000021329 brown rice Nutrition 0.000 title claims abstract description 61
- 102000008214 Glutamate decarboxylase Human genes 0.000 title claims abstract description 28
- 108091022930 Glutamate decarboxylase Proteins 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 24
- 235000013339 cereals Nutrition 0.000 title abstract description 14
- 230000000694 effects Effects 0.000 title abstract description 5
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 31
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 27
- 238000002791 soaking Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 241000209094 Oryza Species 0.000 claims description 13
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 238000009423 ventilation Methods 0.000 claims description 12
- 239000012535 impurity Substances 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 230000035939 shock Effects 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 6
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000035784 germination Effects 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- 206010036590 Premature baby Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 210000004720 cerebrum Anatomy 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/57—Chemical peeling or cleaning of harvested fruits, vegetables or other foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/20—Malt products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a method for improving activity of glutamic acid decarboxylase (GAD) of immature brown rice grains, belonging to the technical field of agriculture products processing. The method is characterized by taking immature brown rice grains eliminated from a color sorting process of brown rice as a raw material, soaking and changing temperature for sprouting, so as to improve the activity of the GAD of the immature brown rice grains, and facilitate enrichment of gamma-aminobutyric acid (GABA). According to the invention, the immature brown rice grains are utilized in an edible way, the GAD of the sprouted immature brown rice grains has no difference from that of the completely immature brown rice grains, and can reach 25-35U/g, and the content of the GABA is 220-320 microgram/g.
Description
One, technical field
The present invention relates to a kind of method that improves immature kernel brown rice glutamate decarboxylase vigor, belong to technical field of agricultural product process.
Two, background technology
Brown rice germinates and can not only promote its nutritional quality through environment stress, reduces ANFs level, improves mouthfeel, optimizes edible processing characteristics, can also significant enrichment functional health ingredient GABA (GABA).Plant can synthesize GABA by GABA shunt metabolism, and this branch road is taking Pidolidone as substrate, through the synthetic GABA of glutamate decarboxylase (GAD) catalytic decarboxylation.Under adverse environmental factor, plant GAD is active can be improved, thereby GABA is accumulated.GABA is inhibitory neurotransmitter main in mammalian central nervous system, human body is had to the cerebrum blood of improvement and circulate, reduce blood pressure, treat the multiple efficacies such as epilepsy and gout, is approved as new resource food in 2009 by health ministry.At present, to GABA, large quantity research has been carried out in beneficiation technologies in sprouted unpolished rice and products thereof exploitation both at home and abroad, but have not been reported taking immature kernel brown rice as the correlative study of raw material germination enrichment GABA.Immature kernel brown rice is prematurity, not full, and grain of rice outward appearance is all opaque but still has the brown rice seed of edibility, claims again white sheet or blue or green head.At present in actual production, there is 20～30% immature kernel brown rice to be rejected to improve brown rice quality by color selector in process.But, because immature kernel brown rice still has certain edibility and is rich in nutrition, it is directly discarded to not only serious environment pollution and also cause a large amount of foodstuff wastes and huge economic loss.Taking immature kernel brown rice as raw material, adopt biotechnology to improve GAD vigor, orienting enriching GABA, can be and produce the food that is rich in GABA very good material is provided.
Patent (publication number 103181523A, open day on July 3rd, 2013) " paddy endogenous enzyme activation method is produced the method for high GABA functional food material ", patent (publication number CN101294155 disclosed, open day on October 29th, 2008) " a kind of activation method for enzyme of rice bran Glutamic Acid decarboxylase ", patent (publication number CN101273769 disclosed, open day on October 1st, 2008) " a kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid " disclosed, these patents are confined to complete ripeness grain in the selection of raw material.The present invention is taking immature kernel brown rice as object, because of its physicochemical property significantly different from complete ripeness grain, so the variation of duration of germination GAD vigor is different from complete ripeness grain with Activiation method.
In the present invention, immature kernel brown rice is after alternating temperature is processed and germinateed, and GAD vigor significantly improves, and enrichment GABA ability strengthens, thereby edibleization of the present invention immature kernel brown rice that utilizes brown rice look to select to reject in process, promotes its economic worth.
Three, summary of the invention
Technical problem
The invention provides a kind of method that improves immature kernel brown rice GAD vigor, after the immature kernel brown rice of selecting removal of impurities is soaked, adopt the mode of alternating temperature processing to cultivate germination, activate GAD, make its activity level that reaches complete ripeness grain brown rice, to improve GABA enriching quantity, and in actual production, substitute complete ripeness grain brown rice, the immature kernel brown rice that edibleization utilized brown rice look to select to reject in process, and then promote its economic worth.
Technical scheme:
After the immature kernel brown rice of selecting removal of impurities is soaked, adopt the mode of alternating temperature processing to cultivate germination, activate its GAD vigor, to improve GABA enriching quantity.Specifically comprise the following steps:
(1) removal of impurities sterilization: after the removal of impurities of immature kernel brown rice, it is carried out disinfection with liquor natrii hypochloritis by known method, then clean up with distilled water;
(2) soak: immature kernel brown rice is soaked to 4～6h at 15～30 DEG C, and every 2h changes water once during this time, and every 1h passes into air once, throughput 3.0～4.0L/min, duration of ventilation is 5～10min;
(3) Fluctuation temperature culture: immature kernel brown rice is cultivated to 3～6h, then cold shock 1.5～2h under 3～5 DEG C of low temperature in 40～50 DEG C under moisture state; Immature kernel brown rice is immersed in the aqueous citric acid solution that pH is 2～3 and supports 48～72h in 25～35 DEG C of band water plantings, then temperature is increased to 40～60 DEG C, continue band water planting and support 2～6h; Immature kernel brown rice band water planting passes into air once every 20～30min during supporting, throughput 2.0～3.0L/min, and duration of ventilation is 5～10min, makes the neat immature kernel sprouted unpolished rice of sprout.
Beneficial effect:
Compared with prior art, the method that the present invention improves immature kernel brown rice GAD vigor has the following advantages:
(1) utilize to edibleization of the present invention brown rice look to select the immature kernel of rejecting in process, promote its economic worth;
(2) in the present invention, immature kernel brown rice is cultivated germination through the mode of alternating temperature processing, can significantly improve its GAD vigor, reaches complete ripeness grain brown rice activity level, and enrichment GABA ability strengthens;
(3) the present invention can improve immature kernel brown rice quality, improves palatability, expands the edible approach of immature kernel brown rice.
Four, detailed description of the invention
Embodiment 1
After the removal of impurities of immature kernel brown rice, it is carried out disinfection with liquor natrii hypochloritis by known method, then clean up with distilled water, at 15 DEG C, soak 6h, every 2h changes water once during this time, and every 1h passes into air once, throughput 3.0L/min, and duration of ventilation is 10min; Immature kernel brown rice after soaking is cultivated to 6h, then cold shock 2h under 3 DEG C of low temperature in 40 DEG C under moisture state; Immerse subsequently pH and be in 2～3 aqueous citric acid solution and support 72h in 25 DEG C of band water plantings, temperature is increased to 40 DEG C, continue band water planting and support 6h; Immature kernel brown rice band water planting passes into air once every 20min during supporting, throughput 3.0L/min, and duration of ventilation is 5min, makes the neat immature kernel sprouted unpolished rice of sprout, and GAD vigor reaches 25U/g, and GABA content is 220 μ g/g.
Embodiment 2
The removal of impurities of immature kernel brown rice, sterilization, cleaning method, with example 1, then soak 5h at 20 DEG C, and every 2h changes water once during this time, and every 1h passes into air once, throughput 3.5L/min, and duration of ventilation is 8min; Immature kernel brown rice after soaking is cultivated to 5h, then cold shock 1.8h under 4 DEG C of low temperature in 45 DEG C under moisture state; Immerse subsequently pH and be in 2～3 aqueous citric acid solution and support 60h in 30 DEG C of band water plantings, temperature is increased to 50 DEG C, continue band water planting and support 4h; Immature kernel brown rice band water planting passes into air once every 25min during supporting, throughput 2.5L/min, and duration of ventilation is 8min, makes the neat immature kernel sprouted unpolished rice of sprout, and GAD vigor reaches 28U/g, and GABA content is 260 μ g/g.
Embodiment 3
The removal of impurities of immature kernel brown rice, sterilization, cleaning method, with example 1, then soak 4h at 30 DEG C, and every 2h changes water once during this time, and every 1h passes into air once, throughput 4.0L/min, and duration of ventilation is 5min; Immature kernel brown rice after soaking is cultivated to 3h, then cold shock 1.5h under 5 DEG C of low temperature in 50 DEG C under moisture state; Immerse subsequently pH and be in 2～3 aqueous citric acid solution and support 48h in 35 DEG C of band water plantings, temperature is increased to 60 DEG C, continue band water planting and support 2h; Immature kernel brown rice band water planting passes into air once every 30min during supporting, throughput 2.0L/min, and duration of ventilation is 10min, makes the neat immature kernel sprouted unpolished rice of sprout, and GAD vigor reaches 35U/g, and GABA content is 320 μ g/g.
Embodiment 4
The removal of impurities of immature kernel brown rice, sterilization, cleaning method, with example 1, then soak 5h at 23 DEG C, and every 2h changes water once during this time, and every 1h passes into air once, throughput 3.2L/min, and duration of ventilation is 6min; Immature kernel brown rice after soaking is cultivated to 4h, then cold shock 1.6h under 4.5 DEG C of low temperature in 48 DEG C under moisture state; Immerse subsequently pH and be in 2～3 aqueous citric acid solution and support 60h in 32 DEG C of band water plantings, temperature is increased to 55 DEG C, continue band water planting and support 3h; Immature kernel brown rice band water planting passes into air once every 28min during supporting, throughput 2.2L/min, and duration of ventilation is 6min, makes the neat immature kernel sprouted unpolished rice of sprout, and GAD vigor reaches 32U/g, and GABA content is 280 μ g/g.
More than describe embodiments of the present invention in detail, but this example of just lifting for the ease of understanding should not be considered to be limitation of the scope of the invention.Equally; any person of ordinary skill in the field all can be according to the description of technical scheme of the present invention and preferred embodiment thereof; make various possible being equal to and change or replace, but all these changes or replacement all should belong to the protection domain of claim of the present invention.
Claims (2)
1. one kind is improved the method for immature kernel brown rice glutamate decarboxylase vigor, it is characterized in that: the immature kernel brown rice of removal of impurities sterilization is after water soaking, adopt the mode of alternating temperature processing to germinate, activate its glutamate decarboxylase (GAD) vigor, to improve GABA (GABA) content, specifically comprise the following steps:
(1) removal of impurities sterilization: after the removal of impurities of immature kernel brown rice, it is carried out disinfection with liquor natrii hypochloritis by known method, then clean up with distilled water;
(2) soak: immature kernel brown rice is soaked to 4～6h at 15～30 DEG C, change during this time water once, every 1h passes into air once, throughput 3.0～4.0L/min, duration of ventilation is 5～10min;
(3) Fluctuation temperature culture: immature kernel brown rice is cultivated to 3～6h, then cold shock 1.5～2h under 3～5 DEG C of low temperature in 40～50 DEG C under moisture state; Immature kernel brown rice is immersed in the aqueous citric acid solution that pH is 2～3 and supports 48～72h in 25～35 DEG C of band water plantings, then temperature is increased to 40～60 DEG C, continue band water planting and support 2～6h; During immature kernel brown rice band water planting is supported, every 20～30min passes into air once, throughput 2.0～3.0L/min, and duration of ventilation is 5～10min, makes the neat immature kernel sprouted unpolished rice of sprout.
2. immature kernel brown rice according to claim 1 is polished rice, glutinous rice, long-grained nonglutinous rice, the long 1～2mm of immature kernel sprouted unpolished rice bud making, GAD vigor 25～35U/g, GABA content 220～320 μ g/g.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410163372.6A CN103919056B (en) | 2014-04-23 | 2014-04-23 | A kind of method improving immature kernel brown rice glutamic acid decarboxylase enzyme activity |
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| CN201410163372.6A CN103919056B (en) | 2014-04-23 | 2014-04-23 | A kind of method improving immature kernel brown rice glutamic acid decarboxylase enzyme activity |
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| CN103919056B CN103919056B (en) | 2016-03-30 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621564A (en) * | 2015-02-05 | 2015-05-20 | 南京农业大学 | Dried fig product rich in gamma-aminobutyric acid and production method of dried fig product |
| CN111587990A (en) * | 2020-06-08 | 2020-08-28 | 闽南师范大学 | Method for enriching gamma-aminobutyric acid in germinated brown rice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1751599A (en) * | 2005-10-13 | 2006-03-29 | 南京农业大学 | Method for producing gamma-aminobutyric acid enriched high milled rice, and product thereof |
| CN101273769A (en) * | 2008-04-24 | 2008-10-01 | 江南大学 | Method for preparing germinated brown rice with high-content gamma-aminobutyric acid |
| CN102986898A (en) * | 2012-12-14 | 2013-03-27 | 苏州科谷米业有限公司 | Preparation method of compound type rice bean (sprout) milk |
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2014
- 2014-04-23 CN CN201410163372.6A patent/CN103919056B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1751599A (en) * | 2005-10-13 | 2006-03-29 | 南京农业大学 | Method for producing gamma-aminobutyric acid enriched high milled rice, and product thereof |
| CN101273769A (en) * | 2008-04-24 | 2008-10-01 | 江南大学 | Method for preparing germinated brown rice with high-content gamma-aminobutyric acid |
| CN102986898A (en) * | 2012-12-14 | 2013-03-27 | 苏州科谷米业有限公司 | Preparation method of compound type rice bean (sprout) milk |
Non-Patent Citations (2)
| Title |
|---|
| 白青云等: "低氧通气对发芽粟谷中γ-氨基丁酸含量的影响", 《食品科学》 * |
| 白青云等: "低氧通气对发芽粟谷中γ-氨基丁酸含量的影响", 《食品科学》, vol. 31, no. 09, 31 December 2010 (2010-12-31), pages 49 - 53 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621564A (en) * | 2015-02-05 | 2015-05-20 | 南京农业大学 | Dried fig product rich in gamma-aminobutyric acid and production method of dried fig product |
| CN111587990A (en) * | 2020-06-08 | 2020-08-28 | 闽南师范大学 | Method for enriching gamma-aminobutyric acid in germinated brown rice |
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| CN103919056B (en) | 2016-03-30 |
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