CN103918870B - Dephenolization cottonseed meal protein powder and preparation method thereof - Google Patents
Dephenolization cottonseed meal protein powder and preparation method thereof Download PDFInfo
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- CN103918870B CN103918870B CN201410174480.3A CN201410174480A CN103918870B CN 103918870 B CN103918870 B CN 103918870B CN 201410174480 A CN201410174480 A CN 201410174480A CN 103918870 B CN103918870 B CN 103918870B
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Abstract
The invention discloses dephenolization cottonseed meal protein powder and a preparation method thereof. The method comprises the following steps: firstly, coarsely crushing cottonseed meal, and then adding cellulose and hemicellulase to carry out enzymolysis on a cellulosic material in the cottonseed meal so as to improve the palatability; arranging the cottonseed meal into a circulating fluidized bed equipped with an infrared emission device; carrying out superfine grinding and infrared irradiation on the material in a boiling state by the circulating fluidized bed; destroying the structure of free gossypol in the cottonseed meal so as to reduce the content of the free gossypol to achieve the detoxification effect; finally drying to prepare the dephenolized cottonseed meal protein powder. The dephenolized cottonseed meal protein powder can be applied to the fields of food and feeds.
Description
Technical field
The invention belongs to food processing field, in particular to a kind of fluid bed irradiation cotton cake dregs albumen powder falling phenol and preparation method thereof.
Background technology
Cotton cake dregs is the accessory substance after cottonseed oil expression.The accessory substance that cottonseed gets oil after shelling claims cottonseed cake, the dregs of rice, and the title cake after oil is got in squeezing, squeezes the title dregs of rice after lixiviate or derect seething in advance.In cotton cake dregs, crude protein content is 20-44%, crude fat is 1-7%, crude fibre is 10-20%, cinder is divided into 5-8%, free gossypol to be 1.0-4.8%, and cottonseed protein is being bordering on soybean protein qualitatively, nutritive value is also high than grain protein, have essential amino acid in 8, especially lysine content is up to 6%.At present, China's cottonseed annual production, more than 8,000,000 tons, produces cotton cake dregs more than 4,000,000 tons per year.But containing gossypol in cotton cake dregs, its Free Gossypol is toxic, this significantly limit its application on food, feed.
For the detoxification of cotton cake dregs, carried out large quantifier elimination both at home and abroad, poison-removing method is mainly divided into physics detoxicity method, chemical passivation method, extraction method and biological detoxication method.Chemical passivation method is by forming bound gossoypol with substance reactions such as protein, amino acid, phosphatide, ferrous sulfate mostly, or add alkali and generate salt, thus detoxification, although this method can detoxification, but also cause the loss of nutriment, corrosivity can be produced to equipment with alkali.Extraction method meeting residual organic solvent, cotton cake dregs has peculiar smell.Biological fermentation process detoxification, shortcoming is long processing period, and cost is higher.
Find by retrieving domestic and international prior art, also there is no at present cotton cake dregs powder in infrared irridiation recirculating fluidized bed to the bibliographical information of its detoxification, by the cotton cake dregs powder in infrared irridiation recirculating fluidized bed not prepared by its detoxification to the bibliographical information of cotton cake dregs albumen powder yet.
Summary of the invention
In view of the deficiencies in the prior art, the object of the invention is to provide a kind of fluid bed irradiation cotton cake dregs albumen powder falling phenol and preparation method thereof.Adopt the dephenolization cottonseed cake protein powder prepared of the method, it has, and cost is low, effective, without the advantage of other Substances Pollution, after measured, the dephenolization cottonseed cake protein powder Free Gossypol content after dephenolize is below 0.035%.
In order to realize object of the present invention, the present inventor is studied and persistent exploration by lot of experiments, finally obtains following technical scheme:
A kind of dephenolization cottonseed cake protein powder, described dephenolization cottonseed cake protein powder obtains after putting into the dephenolize of infrared irridiation recirculating fluidized bed irradiation after cotton cake dregs enzymolysis.
Present invention also offers the preparation method of above-mentioned dephenolization cottonseed cake protein powder, the method specifically comprises the steps:
(1) cotton cake dregs is carried out being crushed to 60-100 order, obtain cotton cake dregs powder;
(2) cotton cake dregs powder is added water infiltration, then add enzyme hydrolysis;
(3) material after enzymolysis is positioned in the recirculating fluidized bed being equipped with infrared transmitting device, open fluid bed, and adopting wavelength to be that the infrared ray of 0.85 μm-2.99 μm carries out irradiation material 5.0-11.0h, the material after irradiation is spray-dried, obtains dephenolization cottonseed cake protein powder.
Preferably, the preparation method of dephenolization cottonseed cake protein powder as above, wherein the concrete steps of step (2) are: after cotton cake dregs powder is added water infiltration 3.0-4.0h with the weight ratio of 1:0.8-1.2, add cellulase and hemicellulase, mix, 50-60 DEG C of insulation 10.0-14.0h.
Further preferably, the preparation method of dephenolization cottonseed cake protein powder as above, the enzyme work of wherein said cellulase is 20,000 U/g, and addition is the 0.1%-0.2%(W/W of cotton cake dregs grain weight amount).
Further preferably, the preparation method of dephenolization cottonseed cake protein powder as above, the enzyme work of wherein said hemicellulase is 200,000 U/g, and addition is the 0.05%-0.1%(W/W of cotton cake dregs grain weight amount).
Again further preferably, the preparation method of dephenolization cottonseed cake protein powder as above, wherein adopts infrared ray to carry out irradiation material 6.0-8.0h in step (3).
Compared with prior art, the dephenolization cottonseed cake protein powder that the present invention relates to and preparation technology's tool thereof have the following advantages and progress significantly:
(1) after cotton cake dregs is pulverized, add cellulase and hemicellulase, cellulose substances is degraded to Small molecular monose that body is easy to digest and assimilate and oligosaccharides, improve the palatability of cotton cake dregs albumen powder.
(2) the cotton cake dregs particle being in fluidized state is acted in fluid bed by infrared irridiation, destroy the benzene ring structure of gossypol, thus make free gossypol loss of activity, reach the effect of detoxification, compare other cotton cake dregs detoxification technologies, cost is low, effective, does not affect the local flavor of cotton cake dregs, also can not bring other impurity.
(3) between the cotton cake dregs particle being in fluidized state in recirculating fluidized bed, phase mutual friction and shock make cotton cake dregs particle become more tiny, be beneficial to cellulase and hemicellulase effect.
Detailed description of the invention
Be below specific embodiments of the invention, technical scheme of the present invention done to describing further, the enzyme work of its cellulase is 20,000 U/g, and the enzyme work of hemicellulase is 200,000 U/g, but protection scope of the present invention is not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
embodiment 1
(1) get cotton cake dregs, be crushed to by 60 mesh sieves.
(2) the cotton cake dregs powder 100g after sieving, add water 80g, after infiltrating 4.0h, adds cellulase 0.1g, hemicellulase 0.05g, mix, 55 DEG C of insulation 11.0h.
(3) the cotton cake dregs powder of enzymolysis, is positioned in recirculating fluidized bed, and IR wavelength is set as 0.85 μm, opens infrared generator and fluid bed, and effect 6.0h, the cotton cake dregs after dephenolize is spray-dried, obtains dephenolization cottonseed cake protein powder.
embodiment 2
(1) get cotton cake dregs, pulverize, cross 60 mesh sieves.
(2) get the cotton cake dregs powder 100g by 100 mesh sieves, add water 120g, after infiltrating 3.0h, adds cellulase 0.1g, hemicellulase 0.05g, mix, 55 DEG C of insulation 11.0h.
(3) the cotton cake dregs powder of enzymolysis, is positioned in recirculating fluidized bed, and IR wavelength is set as 0.94 μm, opens infrared generator and fluid bed, and effect 6.0h, the cotton cake dregs after dephenolize is spray-dried, obtains dephenolization cottonseed cake protein powder.
embodiment 3
(1) get cotton cake dregs, pulverize, cross 80 mesh sieves.
(2) get the cotton cake dregs powder 100g by 80 mesh sieves, add water 100g, after infiltrating 4.0h, adds cellulase 0.2g, hemicellulase 0.1g, mix, 55 DEG C of insulation 14.0h.
(3) the cotton cake dregs powder of enzymolysis, is positioned in recirculating fluidized bed, and IR wavelength is set as 1.80 μm, opens infrared generator and fluid bed, and effect 8.0h, the cotton cake dregs after dephenolize is spray-dried, obtains dephenolization cottonseed cake protein powder.
embodiment 4
(1) get cotton cake dregs, pulverize, cross 100 mesh sieves.
(2) get the cotton cake dregs powder 100g by 100 mesh sieves, add water 100g, after infiltrating 4.0h, adds cellulase 0.2g, hemicellulase 0.1g, mix, 55 DEG C of insulation 14.0h.
(3) the cotton cake dregs powder of enzymolysis, is positioned in recirculating fluidized bed, and IR wavelength is set as 2.99 μm, opens infrared generator and fluid bed, and effect 8.0h, the cotton cake dregs after dephenolize is spray-dried, obtains dephenolization cottonseed cake protein powder.
For the dephenolization cottonseed cake protein powder obtained in above 4 embodiments, detect free gossypol content, crude protein content and crude fiber content wherein, result is as shown in the table:
| Item Ingredients | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Crude protein content (%) | 33.95 | 34.19 | 34.41 | 34.46 |
| Crude fiber content (%) | 1.53 | 1.46 | 0.84 | 0.83 |
| Free gossypol content (%) | 0.033 | 0.027 | 0.021 | 0.029 |
Claims (5)
1. a preparation method for dephenolization cottonseed cake protein powder, is characterized in that: described dephenolization cottonseed cake protein powder obtains after putting into the dephenolize of infrared irridiation recirculating fluidized bed irradiation after cotton cake dregs enzymolysis, specifically comprises the steps:
(1) cotton cake dregs is carried out being crushed to 60-100 order, obtain cotton cake dregs powder;
(2) cotton cake dregs powder is added water infiltration, then add enzyme hydrolysis;
(3) material after enzymolysis is positioned in the recirculating fluidized bed being equipped with infrared transmitting device, open fluid bed, and adopting wavelength to be that the infrared ray of 0.85 μm-2.99 μm carries out irradiation material 5.0-11.0h, the material after irradiation is spray-dried, obtains dephenolization cottonseed cake protein powder.
2. the preparation method of dephenolization cottonseed cake protein powder according to claim 1, it is characterized in that the concrete steps of step (2) are: after cotton cake dregs powder is added water infiltration 3.0-4.0h with the weight ratio of 1:0.8-1.2, add cellulase and hemicellulase, mix, 50-60 DEG C of insulation 10.0-14.0h.
3. the preparation method of dephenolization cottonseed cake protein powder according to claim 2, is characterized in that: the enzyme work of described cellulase is 20,000 U/g, and addition is the 0.1%-0.2% of cotton cake dregs grain weight amount.
4. the preparation method of dephenolization cottonseed cake protein powder according to claim 2, is characterized in that: the enzyme work of described hemicellulase is 200,000 U/g, and addition is the 0.05%-0.1% of cotton cake dregs grain weight amount.
5. the preparation method of dephenolization cottonseed cake protein powder according to claim 1, is characterized in that adopting infrared ray to carry out irradiation material 6.0-8.0h in step (3).
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| CN201410174480.3A CN103918870B (en) | 2014-04-29 | 2014-04-29 | Dephenolization cottonseed meal protein powder and preparation method thereof |
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| CN201410174480.3A CN103918870B (en) | 2014-04-29 | 2014-04-29 | Dephenolization cottonseed meal protein powder and preparation method thereof |
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| CN103918870B true CN103918870B (en) | 2015-03-18 |
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| CN109566912A (en) * | 2018-12-03 | 2019-04-05 | 宁波大学 | A kind of feed and preparation method thereof improving opsariichthys bidens immunity |
| CN116210844A (en) * | 2023-03-21 | 2023-06-06 | 西南科技大学 | Technical method for removing anti-nutritional substances in rapeseed cake by gradient irradiation degradation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01300878A (en) * | 1988-05-27 | 1989-12-05 | San Ei Chem Ind Ltd | Method for decomposing gossypol in cottonseed |
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01300878A (en) * | 1988-05-27 | 1989-12-05 | San Ei Chem Ind Ltd | Method for decomposing gossypol in cottonseed |
| CN101530162A (en) * | 2008-03-10 | 2009-09-16 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
Non-Patent Citations (1)
| Title |
|---|
| 棉子饼粕中棉酚的危害与脱毒方法研究进展;王冬梅 等;《湖北农业科学》;20090205;第48卷(第2期);487-492 * |
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