CN103898232A - 一种检测低丰度分子改变的方法 - Google Patents
一种检测低丰度分子改变的方法 Download PDFInfo
- Publication number
- CN103898232A CN103898232A CN201410156661.3A CN201410156661A CN103898232A CN 103898232 A CN103898232 A CN 103898232A CN 201410156661 A CN201410156661 A CN 201410156661A CN 103898232 A CN103898232 A CN 103898232A
- Authority
- CN
- China
- Prior art keywords
- sample
- kras
- transgenation
- detect
- cancer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
反应体系组分 | 加入的体积(ul) |
10×buffer | 2.5 |
dNTPs | 2.5 |
EvaGreen | 1.25 |
上下游引物KRAS-76- F / R | 1.25+1.25 |
Taq酶 | 0.25 |
纯水 | 15 |
样本编号 | 病理诊断 | 测序结果 | digital HRM结果 | 备注 |
A1 | 胃癌 | 野生型 | 野生型 | 见图2所示 |
A2 | 结肠癌 | GGT-GAT | 突变型 | 见图3所示 |
A3 | 直肠癌 | 野生型 | 野生型 | 见图4所示 |
A4 | 食管癌 | 野生型 | 野生型 | 见图5所示 |
A5 | 盆腔癌 | 野生型 | 野生型 | 见图6所示 |
A6 | 胰腺癌 | 野生型 | 野生型 | 见图7所示 |
A7 | 肛管癌 | GGT-GAT | 突变型 | 见图8所示 |
A8 | 乳腺癌 | GGT-AGT | 突变型 | 见图9所示 |
A9 | 肺癌 | GGT-GAT | 突变型 | 见图10所示 |
样品编号 | 浓度(ng/μl) | 纯度(A260/280) |
B1 | 32.6 | 1.343 |
B2 | 33 | 1.365 |
B3 | 23.3 | 1.245 |
B4 | 52.6 | 1.623 |
B5 | 54.9 | 2.598 |
B6 | 203.9 | 2.118 |
样品编号 | 标本年份 |
C1 | 2006 |
C2 | 2006 |
C3 | 2008 |
C4 | 2008 |
C5 | 2010 |
C6 | 2010 |
样品编号 | 浓度(ng/μl) | 纯度(A260/280) |
D1 | 132.6 | 1.843 |
D2 | 233 | 1.765 |
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410156661.3A CN103898232B (zh) | 2014-04-18 | 2014-04-18 | 一种检测低丰度分子改变的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410156661.3A CN103898232B (zh) | 2014-04-18 | 2014-04-18 | 一种检测低丰度分子改变的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103898232A true CN103898232A (zh) | 2014-07-02 |
CN103898232B CN103898232B (zh) | 2016-04-13 |
Family
ID=50989797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410156661.3A Active CN103898232B (zh) | 2014-04-18 | 2014-04-18 | 一种检测低丰度分子改变的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103898232B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017185767A1 (zh) * | 2016-04-29 | 2017-11-02 | 广州市康立明生物科技有限责任公司 | 一组检测Kras基因突变的引物和探针及其试剂盒 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997008344A2 (en) * | 1995-08-30 | 1997-03-06 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
CN102304581A (zh) * | 2011-09-01 | 2012-01-04 | 广州好芝生物科技有限公司 | 用于检测kras基因突变的试剂盒及其检测方法 |
CN103721245A (zh) * | 2014-01-03 | 2014-04-16 | 苏州工业园区为真生物医药科技有限公司 | 胸腺肽制剂用于制备逆转肿瘤相关基因突变的试剂中的应用 |
-
2014
- 2014-04-18 CN CN201410156661.3A patent/CN103898232B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997008344A2 (en) * | 1995-08-30 | 1997-03-06 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
CN102304581A (zh) * | 2011-09-01 | 2012-01-04 | 广州好芝生物科技有限公司 | 用于检测kras基因突变的试剂盒及其检测方法 |
CN103721245A (zh) * | 2014-01-03 | 2014-04-16 | 苏州工业园区为真生物医药科技有限公司 | 胸腺肽制剂用于制备逆转肿瘤相关基因突变的试剂中的应用 |
Non-Patent Citations (1)
Title |
---|
傅新晖等: "利用高分辨率熔解曲线分析法检测结直肠癌中KRAS基因突变", 《中华普通外科学文献(电子版)》, vol. 03, no. 05, 1 October 2009 (2009-10-01), pages 376 - 378 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017185767A1 (zh) * | 2016-04-29 | 2017-11-02 | 广州市康立明生物科技有限责任公司 | 一组检测Kras基因突变的引物和探针及其试剂盒 |
Also Published As
Publication number | Publication date |
---|---|
CN103898232B (zh) | 2016-04-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Kilgour et al. | Liquid biopsy-based biomarkers of treatment response and resistance | |
Pietrasz et al. | Plasma circulating tumor DNA in pancreatic cancer patients is a prognostic marker | |
Bordi et al. | Circulating DNA in diagnosis and monitoring EGFR gene mutations in advanced non-small cell lung cancer | |
Soulières et al. | KRAS mutation testing in the treatment of metastatic colorectal cancer with anti-EGFR therapies | |
Luk et al. | BRAF mutations in non-small cell lung cancer | |
CN104805207A (zh) | 检测kras基因突变的试剂盒及其检测方法 | |
CN106662543B (zh) | 肺癌患者中的非侵入性基因突变检测 | |
CN103710460A (zh) | 定量检测egfr基因突变的试剂盒及其用途 | |
Mariani et al. | Concordant analysis of KRAS status in primary colon carcinoma and matched metastasis | |
CN102304581A (zh) | 用于检测kras基因突变的试剂盒及其检测方法 | |
Murray et al. | Low frequency of somatic mutations in uterine sarcomas: a molecular analysis and review of the literature | |
CN105624274A (zh) | 肿瘤靶向药物相关基因突变高通量检测方法、引物及试剂 | |
Chen et al. | CCDC167 as a potential therapeutic target and regulator of cell cycle-related networks in breast cancer | |
Rosell et al. | Non-small-cell lung cancer harbouring mutations in the EGFR kinase domain | |
CN104017887A (zh) | 人类braf基因突变检测引物对和探针及其试剂盒 | |
Bhardwaj et al. | Liquid biopsy in ovarian cancer | |
Sattar et al. | Diagnostic and prognostic biomarkers in colorectal cancer and the potential role of exosomes in drug delivery | |
Smolle et al. | Liquid biopsy in non-small cell lung cancer—current status and future outlook—a narrative review | |
Wang et al. | Expression and prognostic value of miRNA-29b in peripheral blood for endometrial cancer | |
Ličar et al. | Distribution of some activating KRAS and BRAF mutations in Slovene patients with colorectal cancer | |
CN107641649B (zh) | 检测微卫星nr27位点稳定性的引物对、试剂盒及方法 | |
Qi et al. | Blood digital polymerase chain reaction as a potential method to detect human epidermal growth factor receptor 2 amplification in non-small cell lung cancer | |
Li et al. | A highly verified assay for KRAS mutation detection in tissue and plasma of lung, colorectal, and pancreatic cancer | |
CN103898232B (zh) | 一种检测低丰度分子改变的方法 | |
Xiao et al. | CD44 affects the expression level of FOS‑like antigen 1 in cervical cancer tissues |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: ZOU HONGZHI FU XINHUI Effective date: 20150427 Owner name: GUANGZHOU KANGLIMING BIOLOGICAL TECHNOLOGY LLC Free format text: FORMER OWNER: WANG JIANPING Effective date: 20150427 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 510655 GUANGZHOU, GUANGDONG PROVINCE TO: 510665 GUANGZHOU, GUANGDONG PROVINCE |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20150427 Address after: 132, room 05, front seat, No. 1 Kam Ming Street, Whampoa Road, Tianhe District, Guangdong, Guangzhou, 510665 Applicant after: GUANGZHOU KANGLIMING BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. Address before: Two cross road 510655 Guangdong city of Guangzhou province Tianhe District village of Sixth Affiliated Hospital of Zhongshan University No. 26 Applicant before: Wang Jianping Applicant before: Zou Hongzhi Applicant before: Fu Xinhui |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP02 | Change in the address of a patent holder |
Address after: 510665, A2 building, No. 11, Kaiyuan Avenue, Science City, Guangzhou hi tech Industrial Development Zone, Guangdong, Sixth Patentee after: GUANGZHOU KANGLIMING BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. Address before: 132, room 05, front seat, No. 1 Kam Ming Street, Whampoa Road, Tianhe District, Guangdong, Guangzhou, 510665 Patentee before: GUANGZHOU KANGLIMING BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. |
|
C56 | Change in the name or address of the patentee | ||
CP02 | Change in the address of a patent holder |
Address after: 510530, A2 building, No. 11, Kaiyuan Avenue, Science City, Guangzhou hi tech Industrial Development Zone, Guangdong, Sixth Patentee after: GUANGZHOU KANGLIMING BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. Address before: 510665, A2 building, No. 11, Kaiyuan Avenue, Science City, Guangzhou hi tech Industrial Development Zone, Guangdong, Sixth Patentee before: GUANGZHOU KANGLIMING BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. |
|
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: 510530 6th floor, building A2, 11 Kaiyuan Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee after: Guangzhou kangliming Biotechnology Co.,Ltd. Address before: 510530 6th floor, building A2, 11 Kaiyuan Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee before: Guangzhou Kliming Biotechnology Co.,Ltd. |