CN103898088A - Method for fixing high-concentration CO2 in flue gas by mutating microalgae biomass through nuclear radiation - Google Patents
Method for fixing high-concentration CO2 in flue gas by mutating microalgae biomass through nuclear radiation Download PDFInfo
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Abstract
The invention relates to a biomass energy utilization technology, and aims to provide a method for fixing high-concentration CO2 in flue gas by mutating microalgae biomass through nuclear radiation. The method for fixing high-concentration CO2 in flue gas by mutating microalgae biomass through nuclear radiation comprises the following steps: radiating an original algae liquid by using <60>Co-gamma rays, and putting into an illumination incubator for recovering and culturing; performing repeated inoculation, culture and centrifugation on the algae liquid to obtain an improved mutated alga which can be used for fixing high-concentration CO2 in flue gas; and inoculating the improved mutated alga, culturing, introducing flue gas, and calculating the carbon fixing efficiency by sampling and calculating the differential value between the inlet and the outlet CO2 concentrations. By adopting the method, the growing rate and carbon fixing efficiency of CO2 of 15 percent by volume in power plant flue gas can be increased effectively.
Description
Technical field
The invention relates to biomass energy utilization technologies, particularly the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation
2method.
Background technology
Having a strong impact on of Greenhouse effect makes people's growing interest CO
2reduction of discharging problem, at present CO in atmosphere
2concentration is increased to 380ppm gradually.The growth and breeding of micro-algae is rapid, a large amount of CO of photosynthesis process need consumption
2therefore, utilize the CO in the fixing coal-fired plant flue gas of micro-algae
2, become study hotspot in recent years.Utilize the Financial cost of waste water and gas cultivating microalgae lower, and can be at efficient fixing flue gas CO
2when obtaining environmental benefit, results have micro-algal biomass of higher economic worth, significant for development new forms of energy and low-carbon economy.
For Strain selection, research shows both at home and abroad, no matter is trace amounts of CO in enclosed space
2fixing or stack gases in a large amount of CO
2remove reduction of discharging, green alga and blue-green algae have very large advantage, especially taking the chlorella in green alga as good.But micro-algae is at high concentration CO
2under condition, have the problems such as growth velocity and solid carbon efficiencies are lower, therefore the efficient carbon algae kind admittedly of exploitation and solid carbon technique are key points.
Efficient fixation of C O
2strain selection method mainly contain three aspects:: (1) natural Strain selection: the people such as Sung isolate a kind of chlorella KR-1 from the waters of near power station, the best CO of this algae growth
2concentration is 10%, and now maximum growth rate is 0.667g/Ld; (2) CO
2concentration domestication: the people such as Yun, at Chlorella vulgaris growing period, pass into the gas of constant rate, and CO in gas
2concentration is along with the prolongation of incubation time is incremented to 30%(approximately every 43h, CO gradually by 5%
2concentration increases by 5% or 10%), calculate CO by average life rate and the carbon content of frustule
2fixed efficiency.Cultivating CO after 183h
2when concentration is increased to 20%, obtain maximum CO
2fixed rate is 0.936g/Ld.(3) chemomorphosis: the people such as Kao use ethyl methane sulfonate induction chlorella to undergo mutation, and (moiety of biogas is :～20%CO passing into biogas
2,～70%CH
4, H
2s<50ppm) the screening mutant of orientation in enclosed space, acquisition can enduring high-concentration CO
2mutagenesis body, and be applied to the CO removing in biogas
2, improve the CH in biogas
4the content of gas.
Because the genome sequencing of many micro-algaes such as chlorella still completes far away, diversity to the crucial controlling gene of the solid carbon of micro-algae, evolutionary path and all many-sides such as associated with apparent property thereof also disclose far away, causing being difficult to by simply certain gene being carried out expressing or knocking out operation to realize the effect of the solid carbon performance of improvement, may be an effective way therefore adopt the solid carbon algae kind of core mutafacient system seed selection.
Summary of the invention
Main purpose of the present invention is to overcome deficiency of the prior art, provides one efficiently fixing flue gas CO
2when obtaining environmental benefit, results have the fixing flue gas high concentration CO of micro-algal biomass of higher economic worth
2method.For solving the problems of the technologies described above, solution of the present invention is:
Provide the micro-algal biomass of inducement of nuclear radiation to fix flue gas high concentration CO
2method, specifically comprise the steps:
(1) getting the original algae liquid of 10～20mL and join in centrifuge tube (50mL), is 100～900GY with dosage
60co-gamma-rays carries out radiation 5～90min to the original algae liquid in centrifuge tube, and dose rate is 10～20GY/min; Then the centrifuge tube that the algae liquid after irradiation is housed is placed in illumination box, carry out the renewal cultivation of 30～40 days, renewal cultivation refers to: culture temperature is 20～23 DEG C, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination when photo-irradiation treatment is 800～1200lux;
(2) algae liquid after treatment step 1 is inoculated in triangular flask (1L), and inoculum size is 5%～10%, the SE standard medium volume in inoculation Vee formation bottle is 500～1000mL; Then postvaccinal algae liquid is carried out to amplification culture: be 25～29 DEG C in culture temperature, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination 1000～3000lux when photo-irradiation treatment, and pass into air in triangular flask, cultivate 10～15 days; After amplification culture finishes, by algae liquid centrifugal 5min under 4000rpm, lose supernatant liquor, lower sediment is mutagenesis algae kind;
(3) mutagenesis algae kind step 2 being obtained is inoculated into cylindrical bottle A(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle A is 300～400mL; Then by postvaccinal mutagenesis algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle A
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 6%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then inoculation is had to substratum centrifugal 5min under 4000rpm of mutagenesis algae kind, lose supernatant liquor, lower sediment is mutagenesis and once tames algae kind;
(4) mutagenesis obtaining in step 3 is once tamed to algae kind and is inoculated into cylindrical bottle B(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle B is 300～400mL; Then postvaccinal mutagenesis is once tamed to algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle B
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 10%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then have mutagenesis once to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is mutagenesis secondary domestication algae kind;
(5) the mutagenesis secondary domestication algae kind obtaining in step 4 is inoculated into cylindrical bottle C(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle C is 300～400mL; Then by postvaccinal mutagenesis secondary domestication algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle C
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 15%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then have mutagenesis secondary to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is can fix flue gas high concentration CO
2improvement mutagenesis algae kind;
(6) the improvement mutagenesis algae kind obtaining in step 5 is inoculated into the cylindrical bottle D(600mL of sealing) in, and inoculum size is 5～10%, SE standard medium volume after inoculation in cylindrical bottle D is 500～600mL, the bottle mouth position of described cylindrical bottle D is opened two ventages, is respectively gas feed and pneumatic outlet; Then by postvaccinal improvement mutagenesis algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into flue gas in cylindrical bottle D, and the speed that passes into that ensures flue gas is between 50～60mL/min, the then flue gas CO of the gas feed to cylindrical bottle D and gas outlet
2concentration detects, by calculating CO
2import and export concentration difference Sampling calculate solid carbon efficiencies.
As further improvement, the algae kind of the original algae liquid in described step 1, comprises the natural algae kind that screening obtains from physical environment, the algae kind mutant that physical chemistry mutagenesis obtains, the transgenic alga kind obtaining through improvement of genes.
As further improvement, the original algae liquid in described step 1 comprises green alga (comprising chlorella, micro-plan ball algae), blue-green algae (comprising quarrel algae, Chaetoceros) and diatom (comprising spirulina, Microcystis aeruginosa).
As further improvement, the original algae liquid in described step 1 comprises micro-algal biomass of spontaneous growth in physical environment, manually the algae liquid of open pond or closed reactor culturing.
As further improvement, the SE standard medium in described step 2～6, consists of: 0.25g NaNO
3, 0.075g K2HPO43H2O, 0.075g MgSO47H2O, 0.025g CaCl22H2O, 0.175g KH2PO4,0.025g NaCl, the 40mL soil solution, 0.005g FeCl
36H
2o, 1mL Fe-EDTA, 1mL A
5solution and 958mL distilled water;
The compound method of the described soil solution is: get the soil 500g that does not execute overfertilization and be placed in triangular flask, add 1000 milliliters of distilled water, bottleneck seals with porous plug, in high-pressure steam sterilizing pan, heat 4 hours with 105 DEG C, cooling, precipitate 24 hours, get supernatant liquor and obtain the soil solution;
The compound method of described Fe-EDTA is: take 0.9306g EDTA-Na
2be dissolved in 50ml distilled water, take FeCl
36H
2it is in 0.1mol/LHCl that O0.901g is dissolved in 50ml concentration, then mixes and obtains Fe-EDTA;
Described A
5the composition of solution is: in 100mL distilled water, contain 286mg H
3bO
3, 181mg MnCl
24H
2o, 22mg ZnSO
44H
2o, 7.9mg CuSO
45H
2o and 3.9mg (NH)
6mo
7o
244H
2o.
As further improvement, the method for calculation of the solid carbon efficiencies in described step 6 are:
Compared with prior art, the invention has the beneficial effects as follows:
Pass through
60the core mutagenesis of Co-gamma-rays and high concentration CO
2the method of gradient domestication can effectively improve algae kind 15vol% high concentration CO in power-plant flue gas
2under growth velocity and solid carbon efficiencies; The good solid carbon algae kind obtaining after nuclear radiation, its photosynthetic growth velocity has improved 53.1%, utilizes CO
2high density domestication artifact quality is original 2.15 times, to CO
2fixed rate be 1.538g/(Ld), Gu carbon efficiencies has reached 32.7%.
Brief description of the drawings
Fig. 1 is process flow sheet of the present invention.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the present invention is described in further detail:
The fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation in Fig. 1
2method, specifically comprise the steps:
(1) getting the original algae liquid of 10～20mL (OD680=0.64) joins in the centrifuge tube of 50mL, described original algae liquid comprises micro-algal biomass of spontaneous growth in physical environment, manually opens the algae liquid of pond, closed reactor culturing, the algae kind of original algae liquid comprises the natural algae kind that screening obtains from physical environment, the algae kind mutant that physical chemistry mutagenesis obtains, the transgenic alga kind obtaining through improvement of genes, and original algae liquid comprises green alga, blue-green algae and diatom.Then be 100～900GY with dosage
60co-gamma-rays carries out radiation 5～90min to the original algae liquid in centrifuge tube, and dose rate is 10～20GY/min.Again the centrifuge tube that the algae liquid after irradiation is housed is placed in illumination box, carry out the renewal cultivation of 30～40 days, renewal cultivation refers to: culture temperature is 20～23 DEG C, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination when photo-irradiation treatment is 800～1200lux.
(2) algae liquid after treatment step 1 is inoculated in the triangular flask of 1L, and inoculum size is 5%～10%, the SE standard medium volume in inoculation Vee formation bottle is 500～1000mL.SE standard medium consists of: 0.25g NaNO
3, 0.075g K
2hPO
43H
2o, 0.075g MgSO
47H
2o, 0.025g CaCl
22H
2o, 0.175g KH
2pO
4, 0.025gNaCl, the 40mL soil solution, 0.005g FeCl
36H
2o, 1mL Fe-EDTA, 1mL A
5solution and 958mL distilled water.The compound method of the soil solution is: get garden soil and do not execute overfertilization 500g and be placed in beaker or triangular flask, add 1000 milliliters of distilled water, bottleneck seals with porous plug, in high-pressure steam sterilizing pan, heat 4 hours with 105 DEG C, cooling, precipitate 24 hours, get supernatant liquor and obtain the soil solution.The compound method of Fe-EDTA is: take 0.9306g EDTA-Na2 and be dissolved in 50ml distilled water, taking FeCl3.6H2O0.901g, to be dissolved in 50ml concentration be in 0.1mol/LHCl, then mixes.A
5the main component of solution is: in 100mL distilled water, contain 286mg H
3bO
3, 181mgMnC1
24H
2o, 22mg ZnSO
44H
2o, 7.9mg CuSO
45H
2o and 3.9mg (NH)
6mo
7o
244H
2o.Then postvaccinal algae liquid is carried out to amplification culture: be 25～29 DEG C in culture temperature, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination 1000～3000lux when photo-irradiation treatment, and pass into air in triangular flask, cultivate 10～15 days; After amplification culture finishes, by algae liquid centrifugal 5min under 4000rpm, lose supernatant liquor, lower sediment is mutagenesis algae kind.
(3) mutagenesis algae kind step 2 being obtained is inoculated in the cylindrical bottle A of 400mL, and inoculum size is 5～10%, and the SE standard medium volume after inoculation in cylindrical bottle A is 300～400mL.Then by postvaccinal mutagenesis algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle A
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 6%, and the speed that passes into that ensures mixed gas is between 30～40mL/min.After cultivation finishes, then inoculation is had to substratum centrifugal 5min under 4000rpm of mutagenesis algae kind, lose supernatant liquor, lower sediment is mutagenesis and once tames algae kind.
(4) mutagenesis obtaining in step 3 is once tamed in the cylindrical bottle B that algae kind is inoculated into 400mL, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle B is 300～400mL.Then postvaccinal mutagenesis is once tamed to algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle B
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 10%, and the speed that passes into that ensures mixed gas is between 30～40mL/min.After cultivation finishes, then have mutagenesis once to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is mutagenesis secondary domestication algae kind.
(5) the mutagenesis secondary domestication algae kind obtaining in step 4 is inoculated in the cylindrical bottle C of 400mL, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle C is 300～400mL.Then by postvaccinal mutagenesis secondary domestication algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle C
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 15%, and the speed that passes into that ensures mixed gas is between 30～40mL/min.After cultivation finishes, then have mutagenesis secondary to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is the improvement algae kind that can fix flue gas high concentration CO 2.
(6) the mutagenesis domestication algae kind obtaining in step 5 is inoculated into the cylindrical bottle D(600mL of the sealing of 600mL) in, bottle mouth position is opened two ventages, one is gas feed, one is pneumatic outlet, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle D is 500～600mL.Then by postvaccinal mutagenesis domestication algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into flue gas in cylindrical bottle D, and the speed that passes into that ensures flue gas is between 50～60mL/min, the then flue gas CO of the gas feed to cylindrical bottle D and gas outlet
2concentration detects, by calculating CO
2import and export concentration difference Sampling calculate solid carbon efficiencies.Gu the method for calculation of carbon efficiencies are:
The following examples can make this professional professional and technical personnel's comprehend the present invention, but do not limit the present invention in any way.
Embodiment 1
Get the original chlorella algae liquid of 10mL (OD680=0.64) of artificial open pond culture, comprise the natural algae kind that screening obtains from physical environment, the algae kind mutant that physical chemistry mutagenesis obtains, the transgenic alga kind obtaining through improvement of genes, joining in the centrifuge tube of 50mL, is 100GY with dosage
60co-gamma-rays carries out radiation 5min to algae liquid, and dose rate is 20GY/min, the algae liquid after irradiation is placed on to the renewal cultivation carrying out in illumination box 30 days, and culture temperature is 20 DEG C, intensity of illumination 800lux, Light To Dark Ratio 12h:12h.By the algae liquid inoculation recovering, carry out amplification culture.
With 1L triangular flask, by the algae kind of inoculating after mutagenesis, inoculum size is 5%, the SE standard medium that after inoculation, volume is 500mL.Be 25 DEG C in culture temperature, intensity of illumination 1000lux, passes into air under Light To Dark Ratio 12h:12h condition, cultivates 10 days, and then centrifugal 5min results under 4000rpm, as mutagenesis algae kind for follow-up test.
The mutagenesis algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL, inoculum size is 5%, the SE standard medium that after inoculation, volume is 300mL, 25 DEG C of temperature, intensity of illumination 4500lux, cultivates under 24h illumination condition continuously, and passes into the CO of 6% volumetric concentration of 30mL/min
2(all the other are nitrogen) cultivates 7 days, and then centrifugal 5min results under 4000rpm, once tame algae kind as follow-up test with mutagenesis.
Again once tame algae liquid with the mutagenesis that the cylindrical bottle inoculation of 400mL is gathered in the crops, inoculum size is 5%, the SE standard medium that after inoculation, volume is 300mL, 25 DEG C of temperature, intensity of illumination 4500lux, cultivates under 24h illumination condition continuously, and passes into the CO of 10% volumetric concentration of 30mL/min
2(all the other are nitrogen) cultivates 7 days, and then centrifugal 5min results under 4000rpm, as mutagenesis secondary domestication algae kind for follow-up test.
The last mutagenesis secondary domestication algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL again, inoculum size is 5%, the SE standard medium that after inoculation, volume is 300mL, 25 DEG C of temperature, intensity of illumination 4500lux, cultivates under 24h illumination condition continuously, and passes into the CO of 15% volumetric concentration of 30mL/min
2(all the other are nitrogen) cultivates 7 days, and centrifugal 5min results under 4000rpm obtain mutagenesis domestication algae kind.The algae kind now obtaining is for can fix flue gas high concentration CO
2improvement algae kind.
Algae liquid after the mutagenesis domestication of gathering in the crops with the cylindrical bottle inoculation of 600mL sealing, bottle mouth position is opened two ventages, one is gas feed, and one is pneumatic outlet, and inoculum size is 5%, the SE standard medium that after inoculation, volume is 500mL, 25 DEG C of temperature, intensity of illumination 4500lux, cultivates under 24h illumination condition continuously, and pass into the flue gas of 50mL/min, detect the CO importing and exporting
2concentration.Adopt the import and export CO of reactor
2difference Sampling calculate solid carbon efficiencies.
In the present embodiment, chlorella is replaced to micro-plan ball algae, also can fix flue gas high concentration CO
2.
Embodiment 2
The original quarrel algae of the 15mL algae liquid (OD680=0.64) of getting closed reactor culturing, joins in the centrifuge tube of 50mL, is 500GY with dosage
60co-gamma-rays carries out radiation 33min to algae liquid, and dose rate is 15GY/min, the algae liquid after irradiation is placed on to the renewal cultivation carrying out in illumination box 35 days, and culture temperature is 22 DEG C, and intensity of illumination is 1000lux, Light To Dark Ratio 12h:12h.By the algae liquid inoculation recovering, carry out amplification culture.
With 1L triangular flask, by the algae kind of inoculating after mutagenesis, inoculum size is 8%, the SE standard medium that after inoculation, volume is 800mL.Be 27 in culture temperature, DEG C intensity of illumination 1500lux, passes into air under Light To Dark Ratio 12h:12h condition, cultivates 13 days, and then centrifugal 5min results under 4000rpm, as mutagenesis algae kind for follow-up test.
The mutagenesis algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL, inoculum size is 8%, the SE standard medium that after inoculation, volume is 350mL, in temperature 27, DEG C intensity of illumination 4700lux, cultivates under 24h illumination condition continuously, and passes into the CO of 6% volumetric concentration of 35mL/min
2(all the other are nitrogen) cultivates 9 days, and then centrifugal 5min results under 4000rpm, once tame algae kind as follow-up test with mutagenesis.
Again once tame algae liquid with the mutagenesis that the cylindrical bottle inoculation of 400mL is gathered in the crops, inoculum size is 8%, the SE standard medium that after inoculation, volume is 350mL, in temperature 27, DEG C intensity of illumination 4700lux, cultivates under 24h illumination condition continuously, and passes into the CO of 10% volumetric concentration of 35mL/min
2(all the other are nitrogen) cultivates 9 days, and then centrifugal 5min results under 4000rpm, as mutagenesis secondary domestication algae kind for follow-up test.
The last mutagenesis secondary domestication algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL again, inoculum size is 8%, the SE standard medium that after inoculation, volume is 350mL, in temperature 27, DEG C intensity of illumination 4700lux, cultivates under 24h illumination condition continuously, and passes into the CO of 15% volumetric concentration of 35mL/min
2(all the other are nitrogen) cultivates 9 days, and centrifugal 5min results under 4000rpm obtain mutagenesis domestication algae kind.The algae kind now obtaining is for can fix flue gas high concentration CO
2improvement algae kind.
Algae liquid after the mutagenesis domestication of gathering in the crops with the cylindrical bottle inoculation of 600mL sealing, bottle mouth position is opened two ventages, one is gas feed, and one is pneumatic outlet, and inoculum size is 8%, the SE standard medium that after inoculation, volume is 550mL, in temperature 27, DEG C intensity of illumination 4700lux, cultivates under 24h illumination condition continuously, and pass into the flue gas of 55mL/min, detect the CO importing and exporting
2concentration.Adopt the import and export CO of reactor
2difference Sampling calculate solid carbon efficiencies.
In the present embodiment, quarrel algae is replaced to Chaetoceros, also can fix flue gas high concentration CO
2.
Embodiment 3
The original spirulina algae of the 20mL liquid (OD680=0.64) of getting closed reactor culturing, joins in the centrifuge tube of 50mL, is 900GY with dosage
60co-gamma-rays carries out radiation 90min to algae liquid, and dose rate is 10GY/min, the algae liquid after irradiation is placed on to the renewal cultivation carrying out in illumination box 40 days, and culture temperature is 23, DEG C intensity of illumination 1200lux, Light To Dark Ratio 12h:12h.By the algae liquid inoculation recovering, carry out amplification culture.
With 1L triangular flask, by the algae kind of inoculating after mutagenesis, inoculum size is 10%, the SE standard medium that after inoculation, volume is 1000mL.Be 29 in culture temperature, DEG C intensity of illumination 3000lux, passes into air under Light To Dark Ratio 12h:12h condition, cultivates 15 days, and then centrifugal 5min results under 4000rpm, as mutagenesis algae kind for follow-up test.
The mutagenesis algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL, inoculum size is 10%, the SE standard medium that after inoculation, volume is 400mL, in temperature 29, DEG C intensity of illumination 5000lux, cultivates under 24h illumination condition continuously, and passes into the CO of 6% volumetric concentration of 40mL/min
2(all the other are nitrogen) cultivates 10 days, and then centrifugal 5min results under 4000rpm, once tame algae kind as follow-up test with mutagenesis.
Again once tame algae liquid with the mutagenesis that the cylindrical bottle inoculation of 400mL is gathered in the crops, inoculum size is 10%, the SE standard medium that after inoculation, volume is 400mL, in temperature 29, DEG C intensity of illumination 5000lux, cultivates under 24h illumination condition continuously, and passes into the CO of 10% volumetric concentration of 40mL/min
2(all the other are nitrogen) cultivates 10 days, and then centrifugal 5min results under 4000rpm, as mutagenesis secondary domestication algae kind for follow-up test.
The last mutagenesis secondary domestication algae liquid of gathering in the crops with the cylindrical bottle inoculation of 400mL again, inoculum size is 10%, the SE standard medium that after inoculation, volume is 400mL, in temperature 29, DEG C intensity of illumination 5000lux, cultivates under 24h illumination condition continuously, and passes into the CO of 15% volumetric concentration of 40mL/min
2(all the other are nitrogen) cultivates 10 days, and centrifugal 5min results under 4000rpm obtain mutagenesis domestication algae kind.The algae kind now obtaining is for can fix flue gas high concentration CO
2improvement algae kind.
Algae liquid after the mutagenesis domestication of gathering in the crops with the cylindrical bottle inoculation of 600mL sealing, bottle mouth position is opened two ventages, one is gas feed, and one is pneumatic outlet, and inoculum size is 10%, the SE standard medium that after inoculation, volume is 600mL, in temperature 29, DEG C intensity of illumination 5000lux, cultivates under 24h illumination condition continuously, and pass into the flue gas of 60mL/min, detect the CO importing and exporting
2concentration.Adopt the import and export CO of reactor
2difference Sampling calculate solid carbon efficiencies.
In the present embodiment, spirulina is replaced to Microcystis aeruginosa, also can fix flue gas high concentration CO
2.
Finally, it should be noted that above what enumerate is only specific embodiments of the invention.Obviously, the invention is not restricted to above embodiment, can also have a lot of distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (6)
1. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation
2method, it is characterized in that, specifically comprise the steps:
(1) getting the original algae liquid of 10～20mL and join in centrifuge tube (50mL), is 100～900GY with dosage
60co-gamma-rays carries out radiation 5～90min to the original algae liquid in centrifuge tube, and dose rate is 10～20GY/min; Then the centrifuge tube that the algae liquid after irradiation is housed is placed in illumination box, carry out the renewal cultivation of 30～40 days, renewal cultivation refers to: culture temperature is 20～23 DEG C, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination when photo-irradiation treatment is 800～1200lux;
(2) algae liquid after treatment step 1 is inoculated in triangular flask (1L), and inoculum size is 5%～10%, the SE standard medium volume in inoculation Vee formation bottle is 500～1000mL; Then postvaccinal algae liquid is carried out to amplification culture: be 25～29 DEG C in culture temperature, carry out successively respectively 12 hours photo-irradiation treatment and 12 hours dark processing every day, and intensity of illumination 1000～3000lux when photo-irradiation treatment, and pass into air in triangular flask, cultivate 10～15 days; After amplification culture finishes, by algae liquid centrifugal 5min under 4000rpm, lose supernatant liquor, lower sediment is mutagenesis algae kind;
(3) mutagenesis algae kind step 2 being obtained is inoculated into cylindrical bottle A(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle A is 300～400mL; Then by postvaccinal mutagenesis algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle A
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 6%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then inoculation is had to substratum centrifugal 5min under 4000rpm of mutagenesis algae kind, lose supernatant liquor, lower sediment is mutagenesis and once tames algae kind;
(4) mutagenesis obtaining in step 3 is once tamed to algae kind and is inoculated into cylindrical bottle B(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle B is 300～400mL; Then postvaccinal mutagenesis is once tamed to algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle B
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 10%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then have mutagenesis once to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is mutagenesis secondary domestication algae kind;
(5) the mutagenesis secondary domestication algae kind obtaining in step 4 is inoculated into cylindrical bottle C(400mL) in, and inoculum size is 5～10%, the SE standard medium volume after inoculation in cylindrical bottle C is 300～400mL; Then by postvaccinal mutagenesis secondary domestication algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into CO in cylindrical bottle C
2cultivate 7～10 days with the mixed gas of nitrogen, and CO in mixed gas
2volumetric concentration be 15%, and the speed that passes into that ensures mixed gas is between 30～40mL/min; After cultivation finishes, then have mutagenesis secondary to tame substratum centrifugal 5min under 4000rpm of algae kind inoculation, lose supernatant liquor, lower sediment is can fix flue gas high concentration CO
2improvement mutagenesis algae kind;
(6) the improvement mutagenesis algae kind obtaining in step 5 is inoculated into the cylindrical bottle D(600mL of sealing) in, and inoculum size is 5～10%, SE standard medium volume after inoculation in cylindrical bottle D is 500～600mL, the bottle mouth position of described cylindrical bottle D is opened two ventages, is respectively gas feed and pneumatic outlet; Then by postvaccinal improvement mutagenesis algae kind, be to cultivate under 25～29 DEG C, the intensity of illumination illumination condition that is 4500～5000lux in temperature, and pass into flue gas in cylindrical bottle D, and the speed that passes into that ensures flue gas is between 50～60mL/min, the then flue gas CO of the gas feed to cylindrical bottle D and gas outlet
2concentration detects, by calculating CO
2import and export concentration difference Sampling calculate solid carbon efficiencies.
2. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation according to claim 1
2method, it is characterized in that the algae kind of the original algae liquid in described step 1 comprises screening obtains from physical environment natural algae kind, the algae kind mutant that physical chemistry mutagenesis obtains, the transgenic alga kind obtaining through improvement of genes.
3. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation according to claim 1
2method, it is characterized in that, the original algae liquid in described step 1 comprises green alga (comprising chlorella, micro-plan ball algae), blue-green algae (comprising quarrel algae, Chaetoceros) and diatom (comprising spirulina, Microcystis aeruginosa).
4. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation according to claim 1
2method, it is characterized in that, the original algae liquid in described step 1 comprises micro-algal biomass of spontaneous growth in physical environment, manually the algae liquid of open pond or closed reactor culturing.
5. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation according to claim 1
2method, it is characterized in that, the SE standard medium in described step 2～6, consists of: 0.25g NaNO
3, 0.075gK
2hPO
43H
2o, 0.075g MgSO
47H
2o, 0.025g CaCl
22H
2o, 0.175g KH
2pO
4, 0.025gNaCl, the 40mL soil solution, 0.005g FeCl
36H
2o, 1mL Fe-EDTA, 1mL A
5solution and 958mL distilled water;
The compound method of the described soil solution is: get the soil 500g that does not execute overfertilization and be placed in triangular flask, add 1000 milliliters of distilled water, bottleneck seals with porous plug, in high-pressure steam sterilizing pan, heat 4 hours with 105 DEG C, cooling, precipitate 24 hours, get supernatant liquor and obtain the soil solution;
The compound method of described Fe-EDTA is: take 0.9306g EDTA-Na
2be dissolved in 50ml distilled water, take FeCl
36H
2it is in 0.1mol/LHCl that O0.901g is dissolved in 50ml concentration, then mixes and obtains Fe-EDTA;
Described A
5the composition of solution is: in 100mL distilled water, contain 286mg H
3bO
3, 181mg MnCl
24H
2o, 22mg ZnSO
44H
2o, 7.9mg CuSO
45H
2o and 3.9mg (NH)
6mo
7o
244H
2o.
6. the fixing flue gas high concentration CO of the micro-algal biomass of inducement of nuclear radiation according to claim 1
2method, it is characterized in that, the method for calculation of the solid carbon efficiencies in described step 6 are:
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107841495A (en) * | 2017-11-27 | 2018-03-27 | 浙江大学 | The method of Rapid Screening core mutagenesis carbon sequestration algae strain is electrically separated using photosynthetic oxygen evolution |
| CN107988129A (en) * | 2017-11-27 | 2018-05-04 | 浙江大学 | Improve the method that spirulina key gene improves growth carbon sequestration rate |
| CN108342344A (en) * | 2018-03-29 | 2018-07-31 | 浙江大学 | A method of increasing algal filament screw pitch and length improves growth of spirulina platensis carbon sequestration rate |
| CN110777091A (en) * | 2019-10-31 | 2020-02-11 | 天津大学 | Method for developing efficient BECCS system with bicarbonate radical as ligament |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101412965A (en) * | 2008-11-17 | 2009-04-22 | 武汉钢铁(集团)公司 | Preparation of microalgae for stabilizing carbon dioxide |
-
2014
- 2014-03-14 CN CN201410096508.6A patent/CN103898088A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101412965A (en) * | 2008-11-17 | 2009-04-22 | 武汉钢铁(集团)公司 | Preparation of microalgae for stabilizing carbon dioxide |
Non-Patent Citations (2)
| Title |
|---|
| JUN CHENG ET AL.: "Mutate Chlorella sp. bynuclear irradiation tofixhigh concentrations of CO2", 《BIORESOURCE TECHNOLOGY》 * |
| 黄云: "微藻固定烟气高浓度CO2的藻种改良和过程优化调控", 《中国博士学位论文全文数据库 工程科技I辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107841495A (en) * | 2017-11-27 | 2018-03-27 | 浙江大学 | The method of Rapid Screening core mutagenesis carbon sequestration algae strain is electrically separated using photosynthetic oxygen evolution |
| CN107988129A (en) * | 2017-11-27 | 2018-05-04 | 浙江大学 | Improve the method that spirulina key gene improves growth carbon sequestration rate |
| CN107988129B (en) * | 2017-11-27 | 2020-07-14 | 浙江大学 | Method for improving key enzyme gene of spirulina to increase growth carbon fixation rate |
| CN108342344A (en) * | 2018-03-29 | 2018-07-31 | 浙江大学 | A method of increasing algal filament screw pitch and length improves growth of spirulina platensis carbon sequestration rate |
| CN108342344B (en) * | 2018-03-29 | 2020-10-20 | 浙江大学 | Method for increasing spiral pitch and length of algae filaments and improving growth carbon fixation rate of spirulina |
| CN110777091A (en) * | 2019-10-31 | 2020-02-11 | 天津大学 | Method for developing efficient BECCS system with bicarbonate radical as ligament |
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