CN103893210A - Chicken gallbladder extraction composition and extraction method thereof - Google Patents
Chicken gallbladder extraction composition and extraction method thereof Download PDFInfo
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Abstract
The present invention relates to a chicken gallbladder extraction composition, which comprises, by mass, 45-55% of taurochenodeoxycholic acid, 10-15% of other cholic acids, 5-15% of amino acids, 23-28% of proteins, and 0.5-2% of fat, wherein the chicken gallbladder extraction composition has the high taurochenodeoxycholic acid content, contains plural kinds of the amino acids, has the high amino acid content, has significant efficacy, and has high stability. The present invention further relates to an extraction method for the chicken gallbladder extraction composition. The extraction method comprises: freezing, chopping, melting and pressing chicken gallbladder, filtering to obtain the bile, adding ethanol to extract, standing, carrying out protein precipitation, filtering to obtain the supernatant, adding petroleum ether to extract, collecting the ethanol layer, and carrying out concentration evaporation dryness to obtain the chicken gallbladder extraction composition. The method has characteristics of simple process, easy operation, low cost and short purification period.
Description
Technical field
The present invention relates to a kind of Fel Gallus domesticus and extract compositions, and this Fel Gallus domesticus extracts the extracting method of compositions.
Background technology
Fel Gallus domesticus, cures mainly heat-clearing and toxic substances removing; Expelling phlegm for arresting cough; Improving eyesight.Main pertussis; Chronic bronchitis; Otitis media; Children's's bacillary dysentery; Sand Stranguria; Conjunctival congestion is shed tears; Cataract; Eczema after ear; Hemorrhoid.Usage and dosage: (1) is for oral administration: 1～3 extracting juice sugaring clothes of freshly-slaughtered poultry gallbladder, also can dry levigation or make tablet, or making chicken bile oral liquor; (2) external: appropriate, get freshly-slaughtered poultry bile eye dripping.
Fel Gallus domesticus is mainly containing 4 kinds of bile acids (bile acid): chenodeoxy cholic acid (chenodeoxycholic acid) 80%; cholic acid (cholic acid) 17%; allocholic acid (allocholic acid) 5%; all with taurine (taurine) combination, 3 β, 7 alpha-dihydroxy-s-Δ 4; 5-cholenic acid (3 β; 7 α-dihydroxy-4,5-diene-cholic acid), 3 Alpha-hydroxy-7 ketone group cholanic acids (3 α-hydroxy-7-oxocholanic acid).
Main active in Fel Gallus domesticus is chenodeoxy cholic acid, has following effect:
1, choleretic effect.The important function of animal bile is to promote bile secretion, and the main component chenodeoxy cholic acid (CDCA) of Fel Gallus domesticus also can increase the secretion of bile and cholate.Give monkey oral CDCA150mg every day, except increasing the secretion of bile and lower limb hydrochlorate, also make phospholipid and cholesterol secretion increase, to thering is the Rhesus Macacus in normal bile acid pond, the dissolubility of cholesterol in bile reduced; Give anesthetized dog and the quiet note of bile duct disease dog CDCA, also make bile and cholate secretion increase.CDCA can make Oddi ' the S sphincter dilatation of Fel Sus domestica pipe end, promotes that bile enters duodenum; With remaining with the test of the sphincteral pig ductus choledochus of Oddi ' S specimen, lax this sphincteral effect of CpeA is better than cholic acid, ursodeoxycholic acid and dehydrogenation gallbladder, and not as good as the acid of deoxidation fat.Separately have report, after oral CDCA, in bile, Bile acid obviously changes, and CDCA increases to more than 80% by normal approximately 40%, lower limb acid (CA) and the corresponding reduction of deoxycholic acid (DCA), and right deoxycholic acid (UDCA) and lithocholic acid (LCA) slightly increase.
2, cholelitholysis.CDCA is that a kind of cholesterol is cholelithiasis lytic agent, can change the formation of bile acid in bile, increase bile storage in body, reduce cholesterol biosynthesis and the secretion of liver, bile cholesterol concentration is being shown and reduce, cholelithiasis formation rate is reduced, make again bile cholesterol desaturation simultaneously, thereby established cholelithiasis is dissolved gradually, dwindle, so that disappear.In vitro tests, CDCA and net cholesterol bead or sterin calculus are hatched in buffer, form, and make bead or cholesterol calculus surface dissolution by mixing micelle (mixed-mecelle).With monkey experiment, CDCA can increase bile storehouse, i.e. the ratio of (bile acid ten lecithin)/(cholesterol), and the ability of raising bile dissolving cholesterol, and can reduce cholesterol biosynthesis and increase choleresis.The oral CDCA of Patients with Cholelithiasis reduces the vigor of liver HMG-CoA (HMG-COA) reductase, and cholesterol biosynthesis is reduced.
3, the effect to digestive system.Bile can the water-fast fat of emulsifying, is beneficial to pancreatic lipase to fatty digestion, promotes the absorption of fat digestion product and fatsoluble vitamin (A, D, K, E), and the bile acids such as CA, CDCA also can strengthen the activity of pancreatic lipase.In mouse small intestine, chenodesoxy cholate can resist spasm due to acetic acid choline, and its ID50 is 6.3 × 10-5g/ml, is better than cholate, and not as good as dexycholate and ursodeoxycholic acid salt.Anesthetized dog colon perfusion CDCA, can reduce the absorption of power and water solution matter.The oral CDCA of people can reduce appetite, and easily causes diarrhoea, thereby causes losing weight.
In prior art, in Fel Gallus domesticus extract, the content of chenodeoxy cholic acid is low, and amino acid whose kind and content are low, and drug effect is poor, and extracting method complexity, and the yield of extract is low, and in addition, the poor stability of natural extract, is difficult to time storage.
CN200610046652.4 discloses a kind of method of extracting chenodeoxy cholic acid with Fel Gallus domesticus, get the section of chicken in jelly gallbladder, be heated to 70 ℃, add gained bile total amount 10% sodium hydroxide to boil 24 hours, reflux cooling, being adjusted to pH with hydrochloric acid is 2, obtain paste, wash with water to neutrality, add 95% ethanol of 2 times of amounts of gallbladder cream and the kieselguhr of gallbladder cream 10%, after backflow, add the active carbon of gallbladder cream amount 10%, reflux, let cool rear filtration, filtrate is added to the 120# gasoline defat of equivalent, reflux, leave standstill, isolate ethanol and in reactor evaporate to dryness be washed till neutrality, add again after 2 times of amount 95% dissolve with ethanols, add the barium chloride of evaporate to dryness medication amount 30% and the active carbon of evaporate to dryness medication amount 5%, reflux, heat filter, filtrate is concentrated out to white crystals thing, after cleaning, add 10 times of water gagings and 10% times of amount aqueous sodium carbonate, reflux, filter, it is 2 that filtrate is adjusted to pH, to there is drying precipitate and get final product.The method step is various, uses solvent more, and cost is high, and productive rate is low, and in addition, amino acid whose kind is few, content is very low.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of Fel Gallus domesticus to extract compositions, and the content that this Fel Gallus domesticus extracts Taurochenodeoxycholic Acid in compositions is high, and amino acid whose kind is many, content is high, and drug effect is remarkable, and in addition, the stability that this Fel Gallus domesticus extracts compositions is higher.
The second object of the present invention is to provide foregoing egg to extract the extracting method of compositions; the method technique is simple; easy to operate; cost is low; the purification cycle is short; in the extraction compositions obtaining, effective ingredient Taurochenodeoxycholic Acid content is high, and amino acid whose kind is many, content is higher, and yield is higher.
In order to realize foregoing invention object, the present invention takes following technical scheme:
A kind of Fel Gallus domesticus extracts compositions, by quality percentage composition, comprises Taurochenodeoxycholic Acid 45-55%, other cholic acid 10-15%, aminoacid 5-15%, protein 23-28%, fatty 0.5-2%.
The main active that Fel Gallus domesticus extracts in compositions is Taurochenodeoxycholic Acid; Taurochenodeoxycholic Acid promotes bile secretion; expand bile acid pond; reduce cholesterol biosynthesis and the secretion of liver, bile cholesterol concentration is being shown and reduce, cholelithiasis formation rate is reduced; make again bile cholesterol desaturation simultaneously; and can the water-fast fat of emulsifying, be beneficial to pancreatic lipase to fatty digestion, promote the absorption of fat digestion product and fatsoluble vitamin (A, D, K, E).The content that Fel Gallus domesticus extracts Taurochenodeoxycholic Acid in compositions is higher; its drug effect is stronger; in addition; in Fel Gallus domesticus extraction compositions provided by the invention, amino acid whose content is also higher; between these aminoacid and Taurochenodeoxycholic Acid, there is collaborative facilitation, can further improve the bioavailability of Taurochenodeoxycholic Acid.
Inventor passes through stability experiment; find that Fel Gallus domesticus that the present invention extracts extracts compositions than other Fel Gallus domesticus extract in prior art; there is higher stability; this is because according in the compositions of aforementioned proportion; between Taurochenodeoxycholic Acid molecule, other cholic acid molecules, amino acid molecular, protein molecule and fat molecule according to certain ratio and regularly arranged distribution; and mutually combine by hydrogen bond or pi-pi accumulation effect; form a stable environment, thereby strengthened the stability of Fel Gallus domesticus extraction compositions.
In the present invention, by quality percentage composition, preferred, described Fel Gallus domesticus extracts compositions and comprises Taurochenodeoxycholic Acid 49-53%, other cholic acid 11-14%, aminoacid 8-12%, protein 24-27%, fatty 0.5-1.5%.
Described other cholic acid comprises cholyltaurine, sweet ammonia lithocholic acid and allocholic acid.
In described other cholic acid, cholyltaurine accounts for 75 ~ 80%, and sweet ammonia lithocholic acid accounts for 10 ~ 15%, and allocholic acid accounts for 10 ~ 15%.
In extraction compositions of the present invention, the kind of conjugated bile acids salt is many, total content is high, conjugated bile acids salt belongs to anion and shows activating agent, reducing the capillary while, its function of gallbladder promoting, molten cholelithiasis, effect aid digestion and inhibition gram positive bacteria are obviously better than non-binding type bile acid.
In extraction compositions of the present invention; contain Taurochenodeoxycholic Acid, cholyltaurine, sweet ammonia lithocholic acid and allocholic acid; the kind of this bile acid series is many; its main component Taurochenodeoxycholic Acid function of gallbladder promoting, molten cholelithiasis, aid digestion and suppress gram positive bacteria in, other cholic acid plays extraordinary synergism as cholyltaurine, sweet ammonia lithocholic acid and allocholic acid and Taurochenodeoxycholic Acid.
Described aminoacid comprises aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus provided by the invention extracts the aminoacid that contains multiple needed by human body in compositions, 7 seed amino acids comprising needed by human: lysine, phenylalanine, methionine, threonine, isoleucine, leucine, valine; 2 kinds of semi-dispensable amino acids: arginine and histidine; And 8 kinds of non essential amino acid glycine, alanine, serine, aspartic acid, glutamic acid, proline, tyrosine, cystine.Amino acid whose kind is many,
Described Fel Gallus domesticus extracts the inorganic elements that also comprises 1-2% in compositions.Described inorganic elements comprises phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.These 9 kinds of inorganic microelements that Fel Gallus domesticus extracts in compositions are the necessary trace element of human body, can promote the function of gallbladder promoting of Taurochenodeoxycholic Acid, the effect of dissolving cholelithiasis and facilitating digestion.
Foregoing Fel Gallus domesticus extracts an extracting method for compositions, comprises the following steps:
(1) get Fel Gallus domesticus, freezing, chopping, melts, and squeezing, filters out gallbladder juice;
(2) get Fel Gallus domesticus, add the ethanol of 90-98%, the volume of ethanol is 2 ~ 4 times of Fel Gallus domesticus volume, and stirring at room temperature is placed, and protein precipitation filters, and obtains supernatant;
(3) get supernatant and add petroleum ether to extract, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions.
In described step (2), stirring at room temperature 20-40min, places 12-17h.
In described step (3), adopt 60 ~ 90# petroleum ether to extract.
In described step (3), the 0.3-1 that the volume of described petroleum ether is supernatant doubly.
In described step (3), extract 3 times.
Extracting method of the prior art is because step is various, and the aminoacid kind in Fel Gallus domesticus is more, and wherein part content is lower, in addition, also contains inorganic microelement in Fel Gallus domesticus, in leaching process, easily runs off.Extracting method step of the present invention is simple, so can retain the lot of trace aminoacid and the lot of trace inorganic elements that contain in Fel Gallus domesticus.
Compared with prior art, beneficial effect of the present invention is:
(1) in Fel Gallus domesticus extraction compositions of the present invention, the content of Taurochenodeoxycholic Acid is high, and amino acid whose kind is many, content is high, and drug effect is remarkable, and the stability that this Fel Gallus domesticus extracts compositions is higher.
(2) in Fel Gallus domesticus compositions of the present invention, also contain 9 kinds of inorganic microelements and be the necessary trace element of human body, can promote the function of gallbladder promoting of Taurochenodeoxycholic Acid, the effect of dissolving cholelithiasis and facilitating digestion.
(3) extracting method technique of the present invention is simple, easy to operate, and cost is low; the purification cycle is short, and in the extraction compositions obtaining, effective ingredient Taurochenodeoxycholic Acid content is high, and the aminoacid that human body is necessary and the kind of inorganic elements are many; and yield is higher than 30.7%, and yield is higher.
The specific embodiment
With embodiment, technical scheme of the present invention is further described below; by the advantage contributing to technical scheme of the present invention; effect has further to be understood, and embodiment does not limit protection scope of the present invention, and protection scope of the present invention is decided by claim.
Embodiment 1
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 90% ethanol, the volume of ethanol is 4 times of Fel Gallus domesticus volume, and stirring at room temperature 20min places 12h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 60# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 0.3 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.7648g, in freshly-slaughtered poultry gallbladder, yield is 30.7%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 51%, other cholic acid accounts for 12%, amino acid/11 0%, protein 25%, fat 1%, inorganic elements 1%.In other cholic acid, cholyltaurine accounts for 75%, and sweet ammonia lithocholic acid accounts for 15%, and allocholic acid accounts for 10%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus extracts in compositions and contains following inorganic elements: in inorganic elements, contain phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
Embodiment 2
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 95% ethanol, the volume of ethanol is 3 times of Fel Gallus domesticus volume, and stirring at room temperature 30min places 15h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 90# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 0.5 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.836g.In freshly-slaughtered poultry gallbladder, yield is 31.5%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 45%, other cholic acid accounts for 15%, amino acid/11 5%, protein 23%, fat 2%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Embodiment 3
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 98% ethanol, the volume of ethanol is 2 times of Fel Gallus domesticus volume, stirring at room temperature 40min, and placement-17h, protein precipitation, filters, and obtains supernatant; Get supernatant and add 90# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 1 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.891g.In freshly-slaughtered poultry gallbladder, yield is 32.1%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 55%, other cholic acid accounts for 10%, aminoacid 5%, protein 28%, fat 1.5%, inorganic elements 0.5%.In other cholic acid, cholyltaurine accounts for 80%, and sweet ammonia lithocholic acid accounts for 10%, and allocholic acid accounts for 10%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus extracts in compositions and contains following inorganic elements: in inorganic elements, contain phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
Embodiment 4
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 93% ethanol, the volume of ethanol is 3 times of Fel Gallus domesticus volume, and stirring at room temperature 25min places 15h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 60# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 0.7 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.791g.In freshly-slaughtered poultry gallbladder, yield is 31.0%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 45%, other cholic acid accounts for 15%, amino acid/11 5%, protein 23%, fat 2%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Embodiment 5
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 98% ethanol, the volume of ethanol is 2 times of Fel Gallus domesticus volume, stirring at room temperature 40min, and placement-17h, protein precipitation, filters, and obtains supernatant; Get supernatant and add 90# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 1 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.891g.In freshly-slaughtered poultry gallbladder, yield is 32.1%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 55%, other cholic acid accounts for 10%, aminoacid 5%, protein 28%, fat 1.5%, inorganic elements 0.5%.In other cholic acid, cholyltaurine accounts for 75%, and sweet ammonia lithocholic acid accounts for 10%, and allocholic acid accounts for 15%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus extracts in compositions and contains following inorganic elements: in inorganic elements, contain phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
Embodiment 6
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 92% ethanol, the volume of ethanol is 2 times of Fel Gallus domesticus volume, and stirring at room temperature 40min places 17h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 90# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 1 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.819.In freshly-slaughtered poultry gallbladder, yield is 31.3%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 49%, other cholic acid accounts for 14%, aminoacid 8%, protein 27%, fat 0.5%, inorganic elements 1.5%.
After testing, aminoacid comprises aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Embodiment 7
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 93% ethanol, the volume of ethanol is 2 times of Fel Gallus domesticus volume, and stirring at room temperature 35min places 13h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 60# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 0.5 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 2.936, in freshly-slaughtered poultry gallbladder, yield is 32.6%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 50.5%, other cholic acid accounts for 11%, amino acid/11 2%, protein 24%, fat 0.5%, inorganic elements 2%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus extracts in compositions and contains following inorganic elements: in inorganic elements, contain phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
Embodiment 8
Get fresh Fel Gallus domesticus 9.0kg, freezing, chopping, melts, and squeezing, filters out gallbladder juice; Get Fel Gallus domesticus, add 96% ethanol, the volume of ethanol is 3 times of Fel Gallus domesticus volume, and stirring at room temperature 30min places 16h, and protein precipitation filters, and obtains supernatant; Get supernatant and add 90# petroleum ether extraction 3 times, the volume of petroleum ether is supernatant 0.8 times, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions 3.015.In freshly-slaughtered poultry gallbladder, yield is 33.5%.Fel Gallus domesticus extracts in compositions: Taurochenodeoxycholic Acid accounts for 53%, other cholic acid accounts for 11%, amino acid/11 0%, protein 25%, fat 0.5%, inorganic elements 0.5%.
After testing, in Fel Gallus domesticus extraction compositions, contain following aminoacid: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
Fel Gallus domesticus extracts in compositions and contains following inorganic elements: in inorganic elements, contain phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
Experimental example 1
Pharmacodynamic experiment
1. experiment material
1.1 animals---Wstar rat, body weight 200～250g, male and female half and half, are provided by Jilin University's zoopery center.
Fel Gallus domesticus extraction compositions prepared by 1.2 medicines and reagent embodiment 1, Fel Gallus domesticus extraction compositions prepared by embodiment 2, Fel Gallus domesticus extraction compositions prepared by embodiment 3, the Fel Gallus domesticus cream of preparing according to CN200610046652.4 embodiment, dimethylbenzene are provided by Tianjin chemical reagent two factories.
1.3 instrument YLS-1A Multifunctional mouse autonomic activities monitors, produce in scientific equipment station, Shandong.
2. method and result
2.1 affect 40 of male rats to rat bile secretion, be divided at random matched group, experimental group 1(embodiment 1 Fel Gallus domesticus and extract compositions 6g/kg body weight), experimental group 2(embodiment 2 Fel Gallus domesticus extract compositions 6g/kg body weight), experimental group 3(embodiment 3 Fel Gallus domesticus extract compositions 6g/kg body weight), comparable group (the Fel Gallus domesticus cream 6g/kg body weight of preparing according to CN200610046652.4 embodiment), 8 every group.Before experiment, water is can't help in 2h fasting, every pentobarbital sodium 60mg/kg intramuscular injection anesthesia for Mus when experiment, abdominal cavity is dissected and is found out stomachus pyloricus, upset descendant duodenum mesentery finds ductus choledochus, under it, wear 2 rhizoid toe-ins and prick pars papillaris, do " V " shape otch to liver direction and insert drainage tube bile drainage, yellow green bile to be had flows out fixing drainage tube, collects with beaker.Postoperative with the closed stomach wall of mosquito forceps, cover with saline gauze.After stablizing 20min, first collect 30min bile, then, respectively by duodenum single administration, dosage is as shown in table 2.After administration, 30min, 60min, 90min respectively collect bile one time, record choleresis, obtain each group of mean.The results are shown in Table 1.
Table 1 is on rat bile secretion impact
| Group | n | Dosage | Bile before administration | 30min after administration | 60min after administration | 90min after administration |
| ? | ? | (g/kg) | Secretory volume (ml) | Choleresis (ml) | Choleresis (ml) | Choleresis (ml) |
| Matched group | 8 | - | 0.176±0.01 | 0.179±0.01 | 0.164±0.02 | 0.165±0.02 |
| Experimental group 1 | 8 | 6 | 0.172±0.02 | 0.238±0.4 ** | 0.212±0.04 * | 0.216±0.04 * |
| Experimental group 2 | 8 | 6 | 0.174±0.01 | 0.234±0.03 * | 0.209±0.03 * | 0.218±0.03 |
| Experimental group 3 | 8 | 6 | 0.176±0.02 | 0.230±0.04 * | 0.204±0.02 * | 0.214±0.02 |
| Comparable group | 8 | 6 | 0.174±0.02 | 0.189±0.03 | 0.184±0.02 * | 0.163±0.01 |
Note: with matched group comparison
*p<0.05,
*p<0.01
Above-mentioned experimental result shows, compared with prior art, Fel Gallus domesticus provided by the invention extracts the effect highly significant of compositions aspect function of gallbladder promoting.
The progradation of 2.2 pairs of rat the intestines and stomach
40 of male rats, be divided at random 5 groups, every group 8, fasting 12h before experiment, matched group (normal saline gavage 0.1ml/10g body weight), experimental group 1(embodiment 1 Fel Gallus domesticus extracts compositions gavage 4g/kg body weight), experimental group 2(embodiment 2 Fel Gallus domesticus extract compositions gavage 4g/kg body weight), experimental group 3(embodiment 3 Fel Gallus domesticus extract compositions gavage 4g/kg body weight), comparable group (Fel Gallus domesticus of preparing according to CN200610046652.4 embodiment extracts compositions gavage 4g/kg body weight), after 20min, every mice all gavages the aqueous solution of carbon dust, in carbon dust 3g, after 30min, each Mus is put to death, cut open the belly, measure the percentage rate that the displacement of powdered carbon in intestinal tube accounts for small intestinal total length, in table 2.
The effect of table 2 to gastrointestinal propulsion
Experimental group compares P<0.01 with matched group, comparable group.
Result shows, the difference highly significant (P<0.01) of experimental group and matched group, comparable group, and experimental group 1,2,3 is compared zero difference, Fel Gallus domesticus provided by the invention extracts compositions is obviously better than prior art Fel Gallus domesticus compositions to the progradation of the intestines and stomach.
Experimental example 2
Stability experiment
This test is carried out according to 2005 editions second appendix XIX C medicine stability test guideline of Chinese Pharmacopoeia, by accelerated test and long term test, has measured the content of Taurochenodeoxycholic Acid under the different storage times, the results are shown in Table 3 and table 4.
The Fel Gallus domesticus that sample 1 is prepared for embodiment 1 extracts compositions;
The Fel Gallus domesticus that sample 2 is prepared for embodiment 2 extracts compositions;
The Fel Gallus domesticus that sample 3 is prepared for embodiment 3 extracts compositions;
Sample 4, for the Fel Gallus domesticus of preparing according to the method for CN200610046652.4 embodiment extracts compositions, collects rate as 21.3% take freshly-slaughtered poultry gallbladder.
Table 3, accelerated test result
| ? | 0 month | 1 month | 2 months | 3 months | 6 months | 12 months |
| 1 | 51.0% | 51.1％ | 50.9％ | 50.8％ | 50.5％ | 50.2％ |
| 2 | 45.1％ | 45.1％ | 45.0％ | 44.9％ | 44.6％ | 44.3％ |
| 3 | 55.4％ | 55.3％ | 55.3％ | 55.2％ | 55.0％ | 54.8％ |
| 4 | 41.2％ | 41.1％ | 41.0％ | 40.7％ | 40.3％ | 39.3％ |
Table 4, long-term test results
| ? | 0 month | 3 months | 6 months | 9 months | 12 months | 18 months |
| 1 | 51.0% | 51.0％ | 50.9％ | 50.7％ | 50.6％ | 50.3％ |
| 2 | 45.1％ | 45.1％ | 44.9％ | 44.8％ | 44.7％ | 44.5％ |
| 3 | 55.4％ | 55.2％ | 55.3％ | 55.2％ | 54.9％ | 54.7％ |
| 4 | 41.2％ | 41.0％ | 40.9％ | 40.5％ | 40.0％ | 39.1％ |
This description of test, Fel Gallus domesticus provided by the invention extracts the good stability of compositions, and under acceleration, long term test condition, the changes of contents of Taurochenodeoxycholic Acid is little.And the Fel Gallus domesticus cream poor stability of prior art.
Other embodiments of the invention product has also carried out identical experiment, and obtains the experimental result of same trend, but length limits, and the present invention will not enumerate.
Claims (10)
1. Fel Gallus domesticus extracts a compositions, it is characterized in that, by quality percentage composition, comprises Taurochenodeoxycholic Acid 45-55%, other cholic acid 10-15%, aminoacid 5-15%, protein 23-28%, fatty 0.5-2%.
2. Fel Gallus domesticus according to claim 1 extracts compositions, it is characterized in that, by quality percentage composition, comprises Taurochenodeoxycholic Acid 49-53%, other cholic acid 11-14%, aminoacid 8-12%, protein 24-27%, fatty 0.5-1.5%.
3. Fel Gallus domesticus according to claim 1 and 2 extracts compositions, it is characterized in that, described aminoacid comprises aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, proline.
4. Fel Gallus domesticus according to claim 1 extracts compositions, it is characterized in that, described Fel Gallus domesticus extracts the inorganic elements that also comprises 1-2% in compositions.
5. Fel Gallus domesticus according to claim 4 extracts compositions, it is characterized in that, described inorganic elements comprises phosphorus, calcium, magnesium, ferrum, manganese, boron, copper, cobalt, chromium.
6. Fel Gallus domesticus according to claim 1 extracts compositions, it is characterized in that, described other cholic acid comprises cholyltaurine, sweet ammonia lithocholic acid and allocholic acid.
7. the Fel Gallus domesticus described in claim 1-6 any one extracts an extracting method for compositions, it is characterized in that, comprises the following steps:
(1) get Fel Gallus domesticus, freezing, chopping, melts, and squeezing, filters out gallbladder juice;
(2) get Fel Gallus domesticus, add 90 ~ 98% ethanol, the volume of ethanol is 2 ~ 4 times of Fel Gallus domesticus volume, and stirring at room temperature is placed, and protein precipitation filters, and obtains supernatant;
(3) get supernatant and add petroleum ether to extract, collect alcohol layer, concentrated evaporate to dryness, obtains Fel Gallus domesticus and extracts compositions.
8. extracting method according to claim 7, is characterized in that, in described step (2), stirring at room temperature 20-40min, places 12-17h.
9. extracting method according to claim 7, is characterized in that, in described step (3), adopts 60 ~ 90# petroleum ether to extract.
10. extracting method according to claim 7, is characterized in that, in described step (3), the 0.3-1 that the volume of described petroleum ether is supernatant doubly.
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| US20210015874A1 (en) * | 2019-07-17 | 2021-01-21 | Shanghai Kaibao Pharmaceutical Co., Ltd. | Use of bio-transformed bear bile powder in preparation of anti-inflammatory drugs |
| CN116898880A (en) * | 2023-09-13 | 2023-10-20 | 江西邦泰绿色生物合成生态产业园发展有限公司 | Preparation method of artificial bear gall powder |
| CN116898880B (en) * | 2023-09-13 | 2024-01-12 | 江西邦泰绿色生物合成生态产业园发展有限公司 | Preparation method of artificial bear gall powder |
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