CN104398694A - Traditional Chinese medicine composition with effect of protecting liver - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition with an effect of protecting the liver. The composition comprises the following raw materials in parts by weight: 4-10 parts of fingered citron, 2-6 parts of chayote leaves, 2-5 parts of astragalus membranaceus, 3-5 parts of rhizoma alismatis, 2-4 parts of dandelion, 1-5 parts of root of kudzu vine, 2-7 parts of momordica grosvenori, 2-8 parts of alder, 3-10 parts of lotus leaves, 2-6 parts of seaweeds, 3-6 parts of loquat leaves and 1-3 parts of liquorice. According to the composition, the fingered citron is taken as a monarch ingredient, the chayote leaves are taken as a ministerial ingredient, and ten traditional Chinese medicines are supplemented; the raw materials are combined for promoting liver tissue recovery, strengthening toxic removal and enhancing liver tissue stability; the composition has a very good liver-protecting effect.

Description

A kind of Chinese medicine composition with liver protection function

Technical field

The invention belongs to technical field of Chinese medicines, be specifically related to a kind of Chinese medicine composition with liver protection function.

Background technology

Liver is the maximum parenchymatous organ of intraperitoneal, undertakes human body important physiological function, and not only secretion of bile participates in digestion activity, and nutritious substance metabolism, storage glycogen, detoxifies, engulfs the important functions such as defence.Because liver is the most important removing toxic substances organ of human body, the chemical reaction such as all noxious substances entering human body or produce in human body metabolism's process all can be oxidized at liver, reduce, hydrolysis, becomes nontoxic or material that toxicity is less.The excessive picked-up of ethanol, viral infection, unbalanced dietary habit, pressure and smoking liver are very easily subject to the infringement of noxious substance, the capillary permeability of liver is caused to increase, hepatocellular degeneration swelling, liver internal hemorrhage, inflammatory cell infiltration, cause liver enlargement, normal function fails, and severe patient even develops into liver cirrhosis, hepatocarcinoma or liver failure.At present, various health product and the Chinese medicine preparation with hepatoprotective function on market, adopt the extract such as Cordyceps, Pollen Pini, Ganoderma that economic worth is higher, there is high cost and raw material supply instability, the shortcoming of control of product quality difficulty.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of Chinese medicine composition with liver protection function.

Technical scheme provided by the invention is a kind of Chinese medicine composition with liver protection function, comprises the component of following weight portion: Fructus Citri Sarcodactylis 4 ~ 10 parts, 2 ~ 6 parts, Fructus Sechii edulis leaf, the Radix Astragali 2 ~ 5 parts, Rhizoma Alismatis 3 ~ 5 parts, Herba Taraxaci 2 ~ 4 parts, Radix Puerariae 1 ~ 5 part, Fructus Momordicae 2 ~ 7 parts, Folium Et Cacumen Alni Japonicae 2 ~ 8 parts, 3 ~ 10 parts, Folium Nelumbinis, Sargassum 2 ~ 6 parts, Folium Eriobotryae 3 ~ 6 parts, 1 ~ 3 part, Radix Glycyrrhizae.

As preferably, the weight portion of each raw material is: Fructus Citri Sarcodactylis 8 parts, 4 parts, Fructus Sechii edulis leaf, the Radix Astragali 3 parts, Rhizoma Alismatis 4 parts, Herba Taraxaci 3 parts, Radix Puerariae 2.5 parts, Fructus Momordicae 4 parts, Folium Et Cacumen Alni Japonicae 6 parts, 7 parts, Folium Nelumbinis, Sargassum 4 parts, Folium Eriobotryae 5 parts, 2 parts, Radix Glycyrrhizae.

The preparation method of above-mentioned Chinese medicine composition comprises the steps:

1) fresh Fructus Citri Sarcodactylis is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by all the other raw material co-grindings, the alcohol heating reflux adding 5 ~ 10 times of weight extracts 1 ~ 3 time, extracts 0.5 ~ 2 hour, merge extractive liquid, at every turn, filters, obtains filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

The volumetric concentration of above-mentioned ethanol is 60 ~ 80%.

This prescription with Fructus Citri Sarcodactylis (i.e. Fructus Citri Sarcodactylis) for monarch, the leaf of Fructus Sechii edulis is minister, both squeezing the juice can strengthen the activity of superoxide dismutase, improve the vigor of scavenging free radicals, to anti-oxidizing activities, the removing of free radical can also be accelerated, reduce the generation of lipid peroxidation, thus stabilized cell membrane structure, alleviate the detrimental effect of radical pair hepatic tissue cell.And other components are that assistant makes in this prescription, there is suppression virus, heat-clearing and toxic substances removing, invigorate blood circulation and fall the effect of summer-heat.All medicines use, can play and promote hepatic tissue rehabilitation, and strengthen mediating of toxin, enhance liver is stablized, and has the effect of good hepatoprotective.

Detailed description of the invention

The present invention is further elaborated for following specific embodiment, but not as a limitation of the invention.

Embodiment 1

1) fresh Fructus Citri Sarcodactylis 4kg is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by Fructus Sechii edulis leaf 2kg, Radix Astragali 2kg, Rhizoma Alismatis 3kg, Herba Taraxaci 2kg, Radix Puerariae 1kg, Fructus Momordicae 2kg, Folium Et Cacumen Alni Japonicae 2kg, Folium Nelumbinis 3kg, Sargassum 2kg, Folium Eriobotryae 3kg, Radix Glycyrrhizae 1kg co-grinding, the 60v% alcohol heating reflux adding 5 times of weight extracts 0.5 hour, by extracting liquid filtering, obtain filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

Embodiment 2

1) fresh Fructus Citri Sarcodactylis 10kg is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by Fructus Sechii edulis leaf 6kg, Radix Astragali 5kg, Rhizoma Alismatis 5kg, Herba Taraxaci 4kg, Radix Puerariae 5kg, Fructus Momordicae 7kg, Folium Et Cacumen Alni Japonicae 8kg, Folium Nelumbinis 10kg, Sargassum 6kg, Folium Eriobotryae 6kg, Radix Glycyrrhizae 3kg co-grinding, the 80v% alcohol heating reflux adding 10 times of weight extracts 3 times, each extraction 2 hours, merge extractive liquid, filter, obtain filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

Embodiment 3

1) fresh Fructus Citri Sarcodactylis 8kg is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by Fructus Sechii edulis leaf 4kg, Radix Astragali 3kg, Rhizoma Alismatis 4kg, Herba Taraxaci 3kg, Radix Puerariae 2.5kg, Fructus Momordicae 4kg, Folium Et Cacumen Alni Japonicae 6kg, Folium Nelumbinis 7kg, Sargassum 4kg, Folium Eriobotryae 5kg, Radix Glycyrrhizae 2kg co-grinding, the 70v% alcohol heating reflux adding 5 ~ 10 times of weight extracts 2 times, each extraction 1 hour, merge extractive liquid, filter, obtain filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

Embodiment 4

1) fresh Fructus Citri Sarcodactylis 10kg is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by Fructus Sechii edulis leaf 2kg, Radix Astragali 2kg, Rhizoma Alismatis 5kg, Herba Taraxaci 2kg, Radix Puerariae 5kg, Fructus Momordicae 2kg, Folium Et Cacumen Alni Japonicae 8kg, Folium Nelumbinis 3kg, Sargassum 6kg, Folium Eriobotryae 3kg, Radix Glycyrrhizae 3kg co-grinding, the 80v% alcohol heating reflux adding 5 times of weight extracts 2 hours, merge extractive liquid, filter, obtain filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

For proving the effect of Chinese medicine composition of the present invention, carry out following test:

1, test method: select healthy Kunming mouse 60, male and female half and half, body weight 18 ~ 22g, be divided into 6 groups is at random negative control group, blank group, experimental group 1 ~ 4 respectively; 0.1% carbon tetrachloride solution is made into Oleum Arachidis hypogaeae semen.Experimental group 1 ~ 4 gives the medicine described in embodiment 1 ~ 4 by 20mg/kg gavage respectively; Negative control group and blank group gavage give 20ml/kg normal saline, and once a day, continuous 7 days, 1h after last administration, except to blank, according to except group, all the other respectively organize the 0.1%CCl that equal gavage gives 20ml/kg ₄oleum Arachidis hypogaeae semen.After gavage, water is can't help in the equal fasting of each treated animal, 16h with after through venous blood sampling, separation of serum, measures glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), Serum MDA (MDA) content in serum.

After getting blood, put to death by mice with cervical dislocation, solution takes its complete liver, is placed in 4% formalin.Murine liver tissue fixed, after dehydration, do paraffin section, HE (Hematoxylin-eosin) dyes, and records the Pathological morphologic change such as hepatocyte distortion, necrosis, inflammatory reaction situation with observation by light microscope.

2, result of the test

2.1 each treated animal serum glutamic pyruvic transminases (ALT), glutamic oxaloacetic transaminase, GOT (AST), Serum MDA (MDA) assay the results are shown in Table 1.

Table 1

Group	n	ALT(U/L)	AST(U/L)	MDA(μmol/L)
					Blank group	10	28.6±7.5*	42.6±6.7*	9.04±1.24*
Negative control group	10	93.2±10.4	126.2±22.4	18.21±1.48
					Experimental group 1	10	58.8±3.78**	65.6±7.81*	10.71±2.89**
Experimental group 2	10	43.5±10.78*	62.3±8.91**	10.35±1.48*
					Experimental group 3	10	36.8±13.5**	56.8±10.36*	9.28±1.09**
Experimental group 4	10	59.4±12.78*	63.6±9.81**	11.51±1.98**

Note: compare with negative control group, * P < 0.05, * * P < 0.01.

2.2 hepatic tissue case morphologic observation results

Blank group murine liver tissue clear in structure.The degeneration of negative control group mouse liver cell obvious tumefaction, hepatocyte arrangement disorder, visible point-like hepatic necrosis in lobule, cell boundary is unclear, has balloon sample to attenuate born of the same parents, and occurs a large amount of inflammatory cell infiltration.The degeneration of experimental group 1 ~ 4 mouse liver cell, necrosis, inflammatory reaction obviously alleviate, and hepatocyte arrangement is more regular.Wherein, the swelling and degeneration of experimental group 3 mouse liver cell, necrosis and inflammatory reaction improve best results.

Claims (4)

1. there is a Chinese medicine composition for liver protection function, it is characterized in that: the component comprising following weight portion: Fructus Citri Sarcodactylis 4 ~ 10 parts, 2 ~ 6 parts, Fructus Sechii edulis leaf, the Radix Astragali 2 ~ 5 parts, Rhizoma Alismatis 3 ~ 5 parts, Herba Taraxaci 2 ~ 4 parts, Radix Puerariae 1 ~ 5 part, Fructus Momordicae 2 ~ 7 parts, Folium Et Cacumen Alni Japonicae 2 ~ 8 parts, 3 ~ 10 parts, Folium Nelumbinis, Sargassum 2 ~ 6 parts, Folium Eriobotryae 3 ~ 6 parts, 1 ~ 3 part, Radix Glycyrrhizae.

2. the Chinese medicine composition with liver protection function according to claim 1, is characterized in that: the weight portion of each raw material is: Fructus Citri Sarcodactylis 8 parts, 4 parts, Fructus Sechii edulis leaf, the Radix Astragali 3 parts, Rhizoma Alismatis 4 parts, Herba Taraxaci 3 parts, Radix Puerariae 2.5 parts, Fructus Momordicae 4 parts, Folium Et Cacumen Alni Japonicae 6 parts, 7 parts, Folium Nelumbinis, Sargassum 4 parts, Folium Eriobotryae 5 parts, 2 parts, Radix Glycyrrhizae.

3. the Chinese medicine composition with liver protection function according to claim 1 and 2, is characterized in that: its preparation method comprises the steps:

1) fresh Fructus Citri Sarcodactylis is squeezed the juice, filter, obtain filtrate for subsequent use;

2) by all the other raw material co-grindings, the alcohol heating reflux adding 5 ~ 10 times of weight extracts 1 ~ 3 time, extracts 0.5 ~ 2 hour, merge extractive liquid, at every turn, filters, obtains filtrate for subsequent use;

3) by step 1) and step 2) filtrate merge, be condensed into thick paste, add Mel modulation evenly, make water-honeyed pill, dry, to obtain final product.

4. the Chinese medicine composition with liver protection function according to claim 3, is characterized in that: the volumetric concentration of described ethanol is 60 ~ 80%.