CN103892165A - 具有控释性的叶黄素纳米分散液及制备方法 - Google Patents
具有控释性的叶黄素纳米分散液及制备方法 Download PDFInfo
- Publication number
- CN103892165A CN103892165A CN201210593151.3A CN201210593151A CN103892165A CN 103892165 A CN103892165 A CN 103892165A CN 201210593151 A CN201210593151 A CN 201210593151A CN 103892165 A CN103892165 A CN 103892165A
- Authority
- CN
- China
- Prior art keywords
- dispersion liquid
- xanthophylls
- lutein
- casein
- nano dispersion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006185 dispersion Substances 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 150000003735 xanthophylls Chemical class 0.000 title abstract 12
- 235000008210 xanthophylls Nutrition 0.000 title abstract 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229920001503 Glucan Polymers 0.000 claims abstract description 18
- 239000005018 casein Substances 0.000 claims abstract description 15
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000021240 caseins Nutrition 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229920001577 copolymer Polymers 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 238000000265 homogenisation Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 5
- 239000001656 lutein Substances 0.000 claims description 46
- 229960005375 lutein Drugs 0.000 claims description 46
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 claims description 46
- 235000012680 lutein Nutrition 0.000 claims description 46
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 claims description 46
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 claims description 46
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 claims description 43
- 239000012530 fluid Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 229920001282 polysaccharide Polymers 0.000 claims description 8
- 239000005017 polysaccharide Substances 0.000 claims description 8
- 150000004676 glycans Chemical class 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 230000002496 gastric effect Effects 0.000 claims description 4
- 125000002635 lutein group Chemical group 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 235000018102 proteins Nutrition 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 230000000968 intestinal effect Effects 0.000 abstract description 10
- 235000013305 food Nutrition 0.000 abstract description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract description 4
- 239000002245 particle Substances 0.000 abstract description 3
- 239000000047 product Substances 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 238000005538 encapsulation Methods 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 102000057297 Pepsin A Human genes 0.000 description 3
- 108090000284 Pepsin A Proteins 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000004051 gastric juice Anatomy 0.000 description 3
- 239000000693 micelle Substances 0.000 description 3
- 229940111202 pepsin Drugs 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001338 self-assembly Methods 0.000 description 2
- ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 description 1
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000004700 cellular uptake Effects 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000002296 dynamic light scattering Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- -1 moisture Inorganic materials 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明属于功能性保健食品技术领域，公开一种具有控释性的叶黄素纳米分散液。本发明的步骤如下：将酪蛋白-葡聚糖共聚物溶于水，然后将溶有叶黄素的乙醇相采用注入法在搅拌下快速注入水相，通过旋转蒸发去除体系中的乙醇，再采用超高压均质处理获得叶黄素纳米分散液，经冷冻干燥即得分散性好的粉末。本发明制得的纳米分散液对叶黄素的包封率高于90％，平均粒径110～150nm之间，且叶黄素为无定形态。本发明显著提高了叶黄素的生物可给性，可作为叶黄素补充剂广泛应用于食品、保健品等行业。本发明的特征在于以酪蛋白与葡聚糖的定向接枝共聚物为壁材，可达到控制叶黄素在胃肠道中的释放，并使其均匀分散于肠液中提高生物可给性的目的。
Description
技术领域
本发明属于功能性保健食品技术领域，公开了一种具有控释性的叶黄素纳米分散液及其制备方法。
背景技术
叶黄素是类胡萝卜素家族的重要成员，具有着色和营养强化的双重功效。作为天然着色剂，叶黄素具有着色力强、安全无毒等优点；同时其也具有卓越的抗氧化性，可预防视力退化和失明症，同时延缓自由基氧化引发的心血管等疾病。然而由于叶黄素分子结构中存在多个共轭双键，导致其易受光、氧、水分、重金属等作用而降解，且不溶于水，微溶于脂肪和油，口服后经肠道吸收率低，这极大限制了叶黄素在食品中的应用。
控制释放是指将药物或其它的活性物质与适当的载体按一定的形式制成制剂，控制药物或活性成分在人体内吸收、代谢以及排泄的过程。随着药物输送相关理论、知识和技术突飞猛进的发展，人们认识到在功能食品行业中也可通过构建具有一定结构的载体用于输送营养物质，以达到提高生物利用率，控制释放及增加饱腹感等目的。纳米输送体系在营养素的包埋、增溶、靶向释放等方面表现出良好的特性，因而逐渐受到研究者的重视。药物领域已见关于利用吐温类以及溶血卵磷脂等表面活性剂制得的混合胶束可有效改善叶黄素的溶解性、细胞摄取率和生物利用率的报道，然而这些材料长时间连续使用易产生蓄积毒性，因此不宜广泛应用于食品体系。
天然生物大分子材料如蛋白、多糖不仅具有良好的生物相容性，而且在消化系统内可被降解，适合作为营养素载体。其中，酪蛋白可通过自组装形成胶束，其疏水内核可用于输送脂溶性物质，是营养物质的天然载体。酪蛋白胶束结构也较为稳定，且胶束中包埋的营养物质具有良好的消化率。然而天然酪蛋白在等电点附近pH条件下溶解性和乳化性较差，且对胃蛋白酶敏感。通过美拉德反应定向接枝获得的酪蛋白-葡聚糖共聚物赋予其更加卓越的界面性质，接枝多糖的无规线团结构特质有效改善了酪蛋白的物理稳定性，降低了蛋白对低pH及胃蛋白酶的敏感性，使疏水性营养素在胃液的消化作用下仍能与蛋白有效结合且保持分散状态，以利于肠道中的释放与乳化，以及随后经由小肠粘膜细胞的吸收。而且酪蛋白-葡聚糖共聚物在低于酪蛋白临界胶束浓度条件下可通过自组装形成胶束，因此可将其作为构建叶黄素等脂溶性营养素的理想基质。
发明内容
本发明的目的是提供一种具有控释性的叶黄素纳米分散液，选择美拉德定向接枝的酪蛋白-葡聚糖共聚物为壁材，成分简单，体系性质稳定，实现了叶黄素的保护和控释。
本发明的另一目的在于提供上述纳米分散液的制备方法，采用乙醇注入-超高压均质制备叶黄素纳米分散液。
本发明的技术方案是，具有控释性的叶黄素纳米分散液，由以下成分组成：
芯材为叶黄素；壁材为酪蛋白-葡聚糖共聚物；其中，芯材与壁材的质量比为0.1～1∶10；所述的酪蛋白-葡聚糖共聚物为干热美拉德反应定向接枝产物经超滤分离制得；所述的葡聚糖相对分子质量为20kDa或40kDa。
本发明所采用的另一技术方案是，上述纳米分散液的制备方法，按以下步骤进行：
a.采用干热美拉德反应定向接枝制备酪蛋白-多糖共聚物：将酪蛋白和多糖(m/m＝1/7～1/3)溶于磷酸盐缓冲液中获得酪蛋白浓度为8g/L的溶液，然后冷冻干燥；将干燥后的样品磨细，过120目筛，反应(60℃，相对湿度为78％，pH 7.0)12～24h；超滤(膜的截留分子量为100kDa)去除未反应的酪蛋白及多糖，即得酪蛋白-葡聚糖共聚物。
b.采用乙醇注入-超高压均质制备叶黄素纳米分散液：将上述酪蛋白-葡聚糖共聚物溶于水中(55℃)，其中蛋白浓度1g/L～10g/L；将溶有叶黄素的乙醇溶液(0.5～1g/L)快速注入共聚物水溶液中，再次搅拌(10～30min)，旋转蒸发除去乙醇，迅速冷却，调整pH为4.6；经超高压均质处理(压力为1000～1400bar条件下，循环3～6次)，即得叶黄素纳米分散液。
c.将纳米分散液进行预冷、冷冻干燥(-40～-50℃，50-100Pa，48h)，得到粉末产品。
本发明所得的纳米分散液平均粒径在110～150nm之间，包埋率高于90％，改善了叶黄素的水溶性，并对胃肠液环境具有响应性，可达到控制叶黄素在胃肠道中的释放，并使其均匀分散于肠液中提高生物可给性的目的。干粉中的叶黄素以无定形态存在，复水后平均粒径略有增加。
粒径的测定：采用动态光散射粒度仪测定，测定温度25℃。
包封率的测定：采用十二烷基硫酸钠增溶法结合正己烷萃取法测定。
附图说明
图1为实施例2制备载量为5％的叶黄素纳米分散液的粒度分布曲线。测定平均粒径为141.3nm，多分散指数为0.209，包封率94.2％。
图2为实施例3制备的叶黄素纳米分散液在模拟胃肠液中叶黄素的累积释放率与时间的关系图。
具体实施方式
实施例1
准确称取酪蛋白0.8g、葡聚糖(40kDa)5.6g溶于100mL 1/15mol/L的磷酸盐缓冲液中，搅拌，制成酪蛋白质量浓度为8g/L的均匀溶液，冷冻干燥24h。干燥后的样品磨细，过120目筛，置于培养皿中，用刺孔的铝箔封口后进行反应(60℃，相对湿度为78％，pH为7.0)反应20h时后冷却终止反应。用截流分子量为100kDa的超滤膜反复超滤三次，收集大于100kDa的组分冷冻干燥，即得酪蛋白-葡聚糖共聚物。
实施例2
将上述共聚物溶于55℃水中(酪蛋白含量为1g/L)，搅拌1h后，将0.005g叶黄素溶于乙醇(10mL)后快速注入100mL共聚物水溶液中，继续搅拌30min，旋转蒸发除去乙醇(55℃，真空度0.1MPa)，迅速冷却，调整pH为4.6，然后经超高压均质处理(压力为1000～1400bar条件下，循环5次)即得叶黄素纳米分散液。测得平均粒径141.3nm，多分散指数为0.209，包封率94.2％。
实施例3
将上述共聚物溶于55℃水中(酪蛋白含量为5g/L)，搅拌1h后，将0.025g叶黄素溶于乙醇(50mL)后快速注入100mL共聚物水溶液中，继续搅拌30min，旋转蒸发除去乙醇(55℃，真空度0.1MPa)，迅速冷却，调整pH为4.6，然后经超高压均质处理(压力为1000～1400bar条件下，循环4次)即得叶黄素纳米分散液。测得平均粒径129.2nm，多分散指数为0.321，包封率94.6％。
实施例4
本实例为对本发明的叶黄素纳米分散液的应用试验。
模拟胃液：将7mL浓盐酸，2.0g氯化钠，3.2g胃蛋白酶溶于250mL去离子水，然后用1.0mol/L盐酸调整pH至1.2，用去离子水定容至1000mL。
模拟肠液：将6.8g磷酸二氢钾溶于250mL去离子水，完全溶解后加190mL 0.2mol/L氢氧化钠和400mL的去离子水，加入复合胰酶(10g)、脱氧胆酸(1.25g)混匀，用0.2mol/L氢氧化钠将体系pH值调至7.0，然后定容至1000mL。充分搅拌后，在4000r/min高速离心15min，取上清液为最终模拟肠液。
取50mL实施例1制备的载量5％叶黄素纳米分散液置于烧杯中，移入50mL模拟胃液保持37℃，以150r/min恒速磁力搅拌，每到设定的时刻从模拟胃液中取出一定体积的胃液，并随后补充相同体积的介质。孵育3h后加入0.168mol/L NaHCO3调整pH值为7以终止反应。在上述胃液中继续加入50mL模拟肠液保持37℃，以150r/min恒速磁力搅拌，每到设定的时刻从模拟肠液中取出一定体积的肠液，并随后补充相同体积的介质。孵育3h后90℃加热5min终止反应。采用正己烷洗涤法测定分析样中的叶黄素游离量。
采用如下公式计算叶黄素的累积释放率：
这里，ci和ct分别指在i和t时刻在释放媒介中叶黄素的浓度；Vi指i时刻所取出释放媒介的体积；Vt指t时刻释放媒介的体积(100mL)；M指分散液中叶黄素总含量。
实验结果：模拟胃液中的共聚物在胃蛋白酶和低pH环境下能够保持结构的相对稳定，对叶黄素起保护作用。在模拟肠液中，复合胰酶及胆酸盐能迅速破坏共聚物的结构，使其快速解体并释放出包埋的叶黄素并均匀分散于肠液中以提高生物可给性。因此，酪蛋白-葡聚糖共聚物作为叶黄素的载体能有效调控其在模拟胃肠液中的释放行为，拓宽叶黄素在食品营养补充剂中的应用范围。
Claims (5)
1.一种叶黄素纳米分散液，由以下成份组成：芯材为叶黄素；壁材为酪蛋白-葡聚糖共聚物；其中，芯材与壁材的质量比为0.1～1∶10。
2.按照权利要求1所述的叶黄素纳米分散液，其特征在于，所述的酪蛋白-葡聚糖共聚物为干热美拉德反应定向接枝产物经超滤分离制得。
3.按照权利要求1所述的叶黄素纳米分散液，其特征在于，所述的葡聚糖相对分子质量为20kDa或40kDa。
4.一种权利要求1所述叶黄素纳米分散液的制备方法，其特征在于，该方法按以下步骤进行：
a.采用干热美拉德反应定向接枝制备酪蛋白-多糖共聚物：将酪蛋白和多糖(1/7～1/3，m/m)溶于磷酸盐缓冲液中获得酪蛋白浓度为8g/L的溶液，然后冷冻干燥；将干燥后的样品磨细，过120目筛，反应(60℃，相对湿度为78％，pH 7.0)12～24h；采用超滤装置(膜的截留分子量为100kDa)去除未反应的酪蛋白及多糖，即得酪蛋白-葡聚糖共聚物。
b.采用乙醇注入-超高压均质制备叶黄素纳米分散液：将上述酪蛋白-葡聚糖共聚物溶于水中(40～55℃)，其中蛋白浓度1g/L～10g/L；将溶有叶黄素的乙醇溶液(0.5～1g/L)快速注入共聚物水溶液中，再次搅拌(10～30min)，旋转蒸发除去乙醇，迅速冷却，调整pH为4.6；经超高压均质处理(压力为1000～1400bar条件下，循环3～6次)，即得叶黄素纳米分散液。
c.将纳米分散液进行预冷、冷冻干燥(-40～-50℃，50-100Pa，48h)，得到粉末产品。
5.按照权利要求4所述的制备方法，其特征在于，该体系可有效包埋叶黄素，并对胃肠液环境具有响应性，可在肠道中缓慢释放出叶黄素。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210593151.3A CN103892165A (zh) | 2012-12-26 | 2012-12-26 | 具有控释性的叶黄素纳米分散液及制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210593151.3A CN103892165A (zh) | 2012-12-26 | 2012-12-26 | 具有控释性的叶黄素纳米分散液及制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103892165A true CN103892165A (zh) | 2014-07-02 |
Family
ID=50983995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210593151.3A Pending CN103892165A (zh) | 2012-12-26 | 2012-12-26 | 具有控释性的叶黄素纳米分散液及制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103892165A (zh) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434348A (zh) * | 2015-12-11 | 2016-03-30 | 华南师范大学 | 一种抗脑癌的藻蓝蛋白-葡聚糖-虫草素胶束及其制备方法和应用 |
| WO2017116805A1 (en) * | 2015-12-30 | 2017-07-06 | Abbott Laboratories | Nutritional products comprising lutein |
| CN109805375A (zh) * | 2019-01-28 | 2019-05-28 | 集美大学 | 一种提高视器官抗氧化能力的功能食品及其制备方法 |
| CN111317824A (zh) * | 2020-02-29 | 2020-06-23 | 复旦大学 | 一种载多肽药物的口服纳米制剂及其制备方法 |
| CN112042937A (zh) * | 2020-09-17 | 2020-12-08 | 青岛农业大学 | 一种水溶性叶黄素乳液凝胶及其制备方法 |
| CN114521650A (zh) * | 2022-01-27 | 2022-05-24 | 华南理工大学 | 一种枣杏糖蛋白包载枣杏色素纳米肠道益生菌促进剂及其制备和应用 |
| CN114522195A (zh) * | 2022-01-27 | 2022-05-24 | 华南理工大学 | 一种板栗杏仁糖蛋白包载板栗果皮色素纳米肠道益生菌促进剂及其制备和应用 |
-
2012
- 2012-12-26 CN CN201210593151.3A patent/CN103892165A/zh active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434348A (zh) * | 2015-12-11 | 2016-03-30 | 华南师范大学 | 一种抗脑癌的藻蓝蛋白-葡聚糖-虫草素胶束及其制备方法和应用 |
| CN105434348B (zh) * | 2015-12-11 | 2018-11-09 | 华南师范大学 | 一种抗脑癌的藻蓝蛋白-葡聚糖-虫草素胶束及其制备方法和应用 |
| WO2017116805A1 (en) * | 2015-12-30 | 2017-07-06 | Abbott Laboratories | Nutritional products comprising lutein |
| CN109805375A (zh) * | 2019-01-28 | 2019-05-28 | 集美大学 | 一种提高视器官抗氧化能力的功能食品及其制备方法 |
| CN111317824A (zh) * | 2020-02-29 | 2020-06-23 | 复旦大学 | 一种载多肽药物的口服纳米制剂及其制备方法 |
| CN111317824B (zh) * | 2020-02-29 | 2022-04-12 | 复旦大学 | 一种载多肽药物的口服纳米制剂及其制备方法 |
| CN112042937A (zh) * | 2020-09-17 | 2020-12-08 | 青岛农业大学 | 一种水溶性叶黄素乳液凝胶及其制备方法 |
| CN112042937B (zh) * | 2020-09-17 | 2023-11-21 | 青岛农业大学 | 一种水溶性叶黄素乳液凝胶及其制备方法 |
| CN114521650A (zh) * | 2022-01-27 | 2022-05-24 | 华南理工大学 | 一种枣杏糖蛋白包载枣杏色素纳米肠道益生菌促进剂及其制备和应用 |
| CN114522195A (zh) * | 2022-01-27 | 2022-05-24 | 华南理工大学 | 一种板栗杏仁糖蛋白包载板栗果皮色素纳米肠道益生菌促进剂及其制备和应用 |
| CN114522195B (zh) * | 2022-01-27 | 2024-01-26 | 华南理工大学 | 一种板栗杏仁糖蛋白包载板栗果皮色素纳米肠道益生菌促进剂及其制备和应用 |
| CN114521650B (zh) * | 2022-01-27 | 2024-04-05 | 华南理工大学 | 一种枣杏糖蛋白包载枣杏色素纳米肠道益生菌促进剂及其制备和应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103892165A (zh) | 具有控释性的叶黄素纳米分散液及制备方法 | |
| Zhang et al. | Encapsulation of curcumin using fucoidan stabilized zein nanoparticles: Preparation, characterization, and in vitro release performance | |
| Xiong et al. | Construction of food-grade pH-sensitive nanoparticles for delivering functional food ingredients | |
| Du et al. | A study on the preparation of chitosan-tripolyphosphate nanoparticles and its entrapment mechanism for egg white derived peptides | |
| Ding et al. | Soy protein/soy polysaccharide complex nanogels: Folic acid loading, protection, and controlled delivery | |
| Lee et al. | Physiochemical properties and prolonged release behaviours of chitosan-denatured β-lactoglobulin microcapsules for potential food applications | |
| Luo et al. | Preparation and characterization of zein/chitosan complex for encapsulation of α-tocopherol, and its in vitro controlled release study | |
| CN111317135A (zh) | 多酚改性的玉米醇溶蛋白纳米粒子包埋缓释姜黄素的方法 | |
| CN110897161A (zh) | 一种高荷载姜黄素的大豆多肽基纳米颗粒及其pH驱动制备方法与应用 | |
| Yuan et al. | One-step self-assembly of curcumin-loaded zein/sophorolipid nanoparticles: physicochemical stability, redispersibility, solubility and bioaccessibility | |
| CN104436205B (zh) | 一种以海洋硫酸多糖制备的纳米载体及纳米复合体与应用 | |
| CN104356406B (zh) | 一种明胶‑多糖接枝物包覆多酚类物质的方法 | |
| Li et al. | Improvement of fucoxanthin oral efficacy via vehicles based on gum Arabic, gelatin and alginate hydrogel: Delivery system for oral efficacy enhancement of functional food ingredients | |
| WO2005048998A1 (en) | G i tract delivery systems | |
| CN109588721B (zh) | 一种类胡萝卜素-蛋白微粒及其制备方法和应用 | |
| Zhang et al. | Ethanol-soluble polysaccharide from sugar beet pulp for stabilizing zein nanoparticles and improving encapsulation of curcumin | |
| CN108850790A (zh) | 一种红曲色素微胶囊及其制备方法 | |
| CN107028883A (zh) | 运载姜黄素纳米乳的制备方法 | |
| Zhang et al. | Fabrication and characterization of core-shell gliadin/tremella polysaccharide nanoparticles for curcumin delivery: Encapsulation efficiency, physicochemical stability and bioaccessibility | |
| Zhang et al. | Sodium caseinate decorating on shellac nanoparticles as a stabilizer for the encapsulation of quercetin | |
| CN112655952A (zh) | 一种虾青素藻油高内相乳液及其制备方法 | |
| Mahmoud et al. | Chitin, chitosan and glucan, properties and applications | |
| Shen et al. | Effect of oxidized dextran on the stability of gallic acid-modified chitosan–sodium caseinate nanoparticles | |
| López-Córdoba et al. | Corn starch-calcium alginate matrices for the simultaneous carrying of zinc and yerba mate antioxidants | |
| Mar et al. | Development of alginate/inulin carrier systems containing non-conventional Amazonian berry extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140702 |