CN103891506B - The measuring method of field soybean nodule nitrogen fixation amount - Google Patents
The measuring method of field soybean nodule nitrogen fixation amount Download PDFInfo
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- CN103891506B CN103891506B CN201410120526.3A CN201410120526A CN103891506B CN 103891506 B CN103891506 B CN 103891506B CN 201410120526 A CN201410120526 A CN 201410120526A CN 103891506 B CN103891506 B CN 103891506B
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Abstract
The measuring method of field soybean nodule nitrogen fixation amount, the present invention relates to the measuring method of nodule nitrogen fixation amount.The present invention will solve the inadequate problem of the estimating and measuring method precision of the nodule nitrogen fixation amount of the soybean varieties of existing cultivation.Method: one, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean; Two, destructive sampling, utilizes formulae discovery to go out dross soybean symbiotic nitrogen fixation amount to be measured.The present invention utilizes dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to calculate dross soybean symbiotic nitrogen fixation amount to be measured, replacement costliness
15n dilution method and natural abundance method, and the full nitrogen method of difference that precision is inadequate.Make testing process safer, result is more accurate simultaneously.Simplify the step of determination experiment, increase work efficiency, reduce experimental cost.The method can also be used for the measuring and calculating of the amount of nitrogen fixation of soybean under different times different croplands control measures.The present invention is for calculating field soybean nodule nitrogen fixation amount.
Description
Technical field
The present invention relates to the measuring method of nodule nitrogen fixation amount.
Background technology
Relevant main three kinds of soybean symbiotic nitrogen fixation amount evaluation method at present: 1) be isotope
15n dilution method, 2) N-15 natural abundance method, 3) by the full nitrogen method of difference, the precision of these three kinds of methods all has direct relation with reference Plant choosing, if reference plant is the corresponding homology nearly allelomorph non-nodulating soybean varieties under identical growth conditions, the result so measured will be very accurately, but at present both at home and abroad by the soybean varieties of extensively cultivating, all lack its corresponding homology nearly allelomorph non-nodulating soybean varieties, normally adopt gramineous crop or soybean varieties will to be planted in sand as reference plant, but crop varieties and soil regime change, the symbiotic nitrogen fixation amount of the root nodule of the soybean so estimated, even
15when N mark, still have a long way to go with the field conditions of reality.
Summary of the invention
The present invention will solve the inadequate problem of the estimating and measuring method precision of the nodule nitrogen fixation amount of the soybean varieties of existing cultivation, and provides the measuring method of field soybean nodule nitrogen fixation amount.
The measuring method of field soybean nodule nitrogen fixation amount, specifically carries out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha ~ 30kg/ha, and often kind of soybean varieties plants 2 ridges, ridge ~ 3, the long 2m ~ 3m in every ridge, joins execute phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha ~ 55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha ~ 70kg/ha;
Two, destructiveness sampling is carried out to dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean is detected, utilize formula:
calculate non-nodulating soybean nitrogen to be measured accumulation total amount, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculates dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
The present invention utilizes dross soybean to be measured, the nitrogen accumulation total amount of Clark dross soybean and Clark non-nodulating soybean and amount of application of nitrogen fertilizer all in there being binomial relation, and all reaches maximum when amount of application of nitrogen fertilizer is 20kg/ha.The amount of nitrogen fixation of Clark dross soybean adopts the nitrogen accumulation total amount of Clark dross soybean to deduct to be in the nitrogen accumulation total amount of the Clark non-nodulating soybean under same growing environment condition to obtain, dross soybean varieties to be measured lacks the allelic series non-nodulating soybean varieties of growth under same environmental condition, the response of nitrogen accumulation to nitrogenous fertilizer of Clark dross soybean and local conjunction rich 35 is unanimous on the whole, all reach maximum when amount of application of nitrogen fertilizer is 20kg/ha, show Clark dross soybean and dross soybean to be measured nitrogen accumulation total amount between there is certain proportionate relationship; Clark dross soybean and non-nodulating soybean are the allelic series soybean of growth in same environment, and the difference of both nitrogen accumulation total amounts is mainly from root nodule symbiotic nitrogen fixation, and under same growing environment, their nitrogen accumulation total amount also exists proportionate relationship.Utilize the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, extrapolate non-nodulating soybean nitrogen to be measured accumulation total amount, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculates dross soybean symbiotic nitrogen fixation amount to be measured.
The invention has the beneficial effects as follows: the present invention utilizes dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to calculate dross soybean symbiotic nitrogen fixation amount to be measured, replacement costliness
15n dilution method and natural abundance method, and the full nitrogen method of difference that precision is inadequate.Make testing process safer, result is more accurate simultaneously.Simplify the step of determination experiment, increase work efficiency, reduce experimental cost.The method can also be used for the measuring and calculating of the amount of nitrogen fixation of soybean under different times different croplands control measures.
The present invention is for calculating field soybean nodule nitrogen fixation amount.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the measuring method of present embodiment field soybean nodule nitrogen fixation amount, specifically carry out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha ~ 30kg/ha, and often kind of soybean varieties plants 2 ridges, ridge ~ 3, the long 2m ~ 3m in every ridge, joins execute phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha ~ 55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha ~ 70kg/ha;
Two, destructiveness sampling is carried out to dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean is detected, utilize formula:
calculate non-nodulating soybean nitrogen to be measured accumulation total amount, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculates dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
Embodiment two: present embodiment and embodiment one unlike: in step one, amount of application of nitrogen fertilizer is 15kg/ha ~ 25kg/ha.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: in step one, amount of application of nitrogen fertilizer is 20kg/ha.Other is identical with embodiment one or two.
Embodiment four: present embodiment and embodiment one unlike: in step one, Phosphorus fertilizer usage is 51kg/ha, and K Amounts is 60kg/ha.Other is identical with embodiment one.
Embodiment five: present embodiment and embodiment one unlike: in step 2, the destructive soybean sampled is the maturing stage.Other is identical with embodiment one.
Following examples are adopted to verify beneficial effect of the present invention:
Embodiment one:
The measuring method of the present embodiment field soybean nodule nitrogen fixation amount, specifically carries out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 20kg/ha, and often kind of soybean varieties plants 2 ridges, the long 2m in every ridge, joins execute phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 51kg/ha, potash fertilizer is K
2o, amount of application are 60kg/ha;
Two, destructiveness sampling is carried out to dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean is detected, utilize formula:
calculate non-nodulating soybean nitrogen to be measured accumulation total amount, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculates dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
In the present embodiment, dross soybean to be measured is for closing rich 35, and in step 2, the soybean of destructive sampling is the maturing stage.
It is 144.4kg/ha that the present embodiment utilizes dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to calculate dross soybean symbiotic nitrogen fixation amount to be measured, and adopts expensive simultaneously
15the soybean brother amount of nitrogen fixation of N By Dilution is 158.7kg/ha, and the soybean amount of nitrogen fixation that the present invention can calculate out and the result difference that accurate 15N measures not quite, and adopt the method for this patent to make testing process safer, and result is more accurate.Simplify the step of determination experiment, increase work efficiency, reduce experimental cost.
Claims (5)
1. the measuring method of field soybean nodule nitrogen fixation amount, its feature is specifically carried out according to following steps at this measuring method:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha ~ 30kg/ha, and often kind of soybean varieties plants 2 ridges, ridge ~ 3, the long 2m ~ 3m in every ridge, joins execute phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha ~ 55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha ~ 70kg/ha;
Two, destructiveness sampling is carried out to dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean is detected, utilize formula:
Calculate non-nodulating soybean nitrogen to be measured accumulation total amount, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculate dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount;
Wherein dross soybean to be measured is for closing rich 35.
2. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, is characterized in that in step one, amount of application of nitrogen fertilizer is 15kg/ha ~ 25kg/ha.
3. the measuring method of field soybean nodule nitrogen fixation amount according to claim 2, is characterized in that in step one, amount of application of nitrogen fertilizer is 20kg/ha.
4. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, it is characterized in that in step one, Phosphorus fertilizer usage is 51kg/ha, K Amounts is 60kg/ha.
5. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, is characterized in that carrying out destructiveness sampling to maturing stage soybean in step 2.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007846A (en) * | 2010-11-08 | 2011-04-13 | 中国科学院东北地理与农业生态研究所 | Method for detecting quantity of nitrogen fixed by soybean root nodules |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007846A (en) * | 2010-11-08 | 2011-04-13 | 中国科学院东北地理与农业生态研究所 | Method for detecting quantity of nitrogen fixed by soybean root nodules |
Non-Patent Citations (6)
| Title |
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| Improved estimation of biological nitrogen fixation of soybean cultivars (Glycine max L. Merril) using 15N natural abundance technique;P. Houngnandan等;《Biol Fertil Soils》;20081231;第45卷(第2期);第175-183页 * |
| Nodule Formation and Development in Soybeans (Glycine max L.)in Response to Phosphorus Supply in Solution Culture;MIAO Shu-Jie等;《Pedosphere》;20071231;第17卷(第1期);第36-43页 * |
| 不同大豆品种根瘤固氮酶活性与固氮量差异研究;陈慧等;《核学农报》;20131231;第27卷(第3期);第379-383页 * |
| 利用N-15自然丰度法研究固氮植物生物固氮量;何道文等;《干旱地区农业研究》;20040331;第22卷(第1期);第132-137页 * |
| 张宏等.黑土中土著大豆根瘤菌的固氮(C2H2)活性结瘤性状和固氮量估测.《大豆科学》.1986,第5卷(第1期),第47-56页. * |
| 施氮对结瘤和非结瘤近等位基因大豆生长和固氮性状的影响;石海等;《大豆科学》;20121231;第31卷(第6期);第961-965,971页 * |
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