CN103891506A - Measuring and calculating method of field soybean root nodule nitrogen fixation amount - Google Patents
Measuring and calculating method of field soybean root nodule nitrogen fixation amount Download PDFInfo
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Abstract
The invention relates to a measuring and calculating method of field soybean root nodule nitrogen fixation amount and aims to solve the problem that the existing estimating methods of soybean root nodule nitrogen fixation amount is insufficient in precision. The method includes: firstly, planting to-be-measured nodulation soybean, Clark nodulation soybean and Clark non-nodulation soybean; secondly, destructively sampling, and using a formula to calculate the symbiotic nitrogen fixation amount of the nodulation soybean. The method has the advantages that the symbiotic nitrogen fixation amount of the to-be-measured nodulation soybean is measured and calculated by using the to-be-measured nodulation soybean, the Clark nodulation soybean and the Clark non-nodulation soybean, the expensive 15N diluting method, the natural abundance method and the full-nitrogen difference method insufficient in precision are replaced, safe detecting and accurate results are achieved, measuring experiment steps are simplified, work efficiency is increased, and experiment cost is lowered. In addition, the method is applicable to measuring and calculating the nitrogen fixation amount of soybean under different stages and different field managing measures.
Description
Technical field
The present invention relates to the measuring method of nodule nitrogen fixation amount.
Background technology
Main three kinds of at present relevant soybean symbiotic nitrogen fixation amount evaluation method: 1) be isotope
15n dilution method, 2) N-15 natural abundance method, 3) by the full nitrogen method of difference, the precision of these three kinds of methods all selects to have direct relation with reference plant, if reference plant is the nearly allelomorph non-nodulating of the corresponding homology soybean varieties under isometric growth condition, the result of measuring so will be very accurately, but the soybean varieties of extensively being cultivated both at home and abroad at present, all lack the nearly allelomorph non-nodulating of its corresponding homology soybean varieties, normally adopt gramineous crop or will be planted in sand soybean varieties as reference plant, but crop varieties and soil regime change, the symbiotic nitrogen fixation amount of the root nodule of the soybean of estimation so, even
15in the situation of N mark, still have a long way to go with actual field situation.
Summary of the invention
The present invention will solve the inadequate problem of estimating and measuring method precision of the nodule nitrogen fixation amount of the soybean varieties of existing cultivation, and the measuring method of field soybean nodule nitrogen fixation amount is provided.
The measuring method of field soybean nodule nitrogen fixation amount, specifically carries out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha～30kg/ha, and every kind of soybean varieties is planted 2 ridges, ridge～3, and the long 2m～3m in every ridge joined and executes phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha～55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha～70kg/ha;
Two, dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are carried out to destructiveness sampling, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are detected, utilize formula:
calculate non-nodulating soybean nitrogen accumulation total amount to be measured, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculate dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
The present invention utilizes the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to be all and to have binomial relation with amount of application of nitrogen fertilizer, and all in the time that amount of application of nitrogen fertilizer is 20kg/ha, reaches maximum.The amount of nitrogen fixation of Clark dross soybean is to adopt the nitrogen accumulation total amount of Clark dross soybean to deduct the nitrogen accumulation total amount acquisition of the Clark non-nodulating soybean under same growing environment condition, dross soybean varieties to be measured lacks the allelic series non-nodulating soybean varieties being grown under same environmental condition, Clark dross soybean and local rich 35 the nitrogen accumulation of closing are unanimous on the whole to the response of nitrogenous fertilizer, all in the time that amount of application of nitrogen fertilizer is 20kg/ha, reach maximum, show to have certain proportionate relationship between the nitrogen accumulation total amount of Clark dross soybean and dross soybean to be measured; Clark dross soybean and non-nodulating soybean are the allelic series soybean being grown in same environment, and the difference of both nitrogen accumulation total amounts is mainly from root nodule symbiotic nitrogen fixation, and under same growing environment, their nitrogen accumulation total amount also exists proportionate relationship.Utilize the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, extrapolate non-nodulating soybean nitrogen accumulation total amount to be measured, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculates dross soybean symbiotic nitrogen fixation amount to be measured.
The invention has the beneficial effects as follows: the present invention utilizes dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to calculate dross soybean symbiotic nitrogen fixation amount to be measured, replacement costliness
15n dilution method and natural abundance method, and the inadequate full nitrogen method of difference of precision.Make testing process safer, result is more accurate simultaneously.Simplify the step of determination experiment, increased work efficiency, reduced experimental cost.The method can also be used for the measuring and calculating of the amount of nitrogen fixation of soybean under different times different croplands control measures.
The present invention is used for calculating field soybean nodule nitrogen fixation amount.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the measuring method of present embodiment field soybean nodule nitrogen fixation amount, specifically carries out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha～30kg/ha, and every kind of soybean varieties is planted 2 ridges, ridge～3, and the long 2m～3m in every ridge joined and executes phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha～55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha～70kg/ha;
Two, dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are carried out to destructiveness sampling, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are detected, utilize formula:
calculate non-nodulating soybean nitrogen accumulation total amount to be measured, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculate dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
Embodiment two: present embodiment is different from embodiment one: in step 1, amount of application of nitrogen fertilizer is 15kg/ha～25kg/ha.Other is identical with embodiment one.
Embodiment three: present embodiment is different from embodiment one or two: in step 1, amount of application of nitrogen fertilizer is 20kg/ha.Other is identical with embodiment one or two.
Embodiment four: present embodiment is different from embodiment one: in step 1, phosphate fertilizer amount is 51kg/ha, K Amounts is 60kg/ha.Other is identical with embodiment one.
Embodiment five: present embodiment is different from embodiment one: in step 2, the soybean of destructive sampling is the maturing stage.Other is identical with embodiment one.
Adopt following examples to verify beneficial effect of the present invention:
Embodiment mono-:
The measuring method of the present embodiment field soybean nodule nitrogen fixation amount, specifically carries out according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 20kg/ha, and every kind of soybean varieties is planted 2 ridges, and the long 2m in every ridge joined and executes phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 51kg/ha, potash fertilizer is K
2o, amount of application are 60kg/ha;
Two, dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are carried out to destructiveness sampling, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are detected, utilize formula:
calculate non-nodulating soybean nitrogen accumulation total amount to be measured, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculate dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
In the present embodiment, dross soybean to be measured is rich 35 for closing, and in step 2, the soybean of destructive sampling is the maturing stage.
It is 144.4kg/ha that the present embodiment utilizes dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean to calculate dross soybean symbiotic nitrogen fixation amount to be measured, and employing costliness simultaneously
15the soybean brother amount of nitrogen fixation of N By Dilution is 158.7kg/ha, and the soybean amount of nitrogen fixation that the present invention can calculate is out little with the result difference that accurate 15N measures, and adopts the method for this patent to make testing process safer, and result is more accurate.Simplify the step of determination experiment, increased work efficiency, reduced experimental cost.
Claims (5)
1. the measuring method of field soybean nodule nitrogen fixation amount, is characterized in that specifically carrying out the measuring method of field soybean nodule nitrogen fixation amount according to following steps:
One, plant dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean, control amount of application of nitrogen fertilizer is 10kg/ha～30kg/ha, and every kind of soybean varieties is planted 2 ridges, ridge～3, and the long 2m～3m in every ridge joined and executes phosphate fertilizer and potash fertilizer in seedling stage, and wherein, phosphate fertilizer is P
2o
5, amount of application is 50kg/ha～55kg/ha, potash fertilizer is K
2o, amount of application are 50kg/ha～70kg/ha;
Two, dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are carried out to destructiveness sampling, respectively the nitrogen accumulation total amount of dross soybean to be measured, Clark dross soybean and Clark non-nodulating soybean are detected, utilize formula:
calculate non-nodulating soybean nitrogen accumulation total amount to be measured, recycling formula: dross soybean symbiotic nitrogen fixation amount to be measured=dross soybean nitrogen accumulation total amount to be measured-non-nodulating soybean nitrogen accumulation total amount to be measured, calculate dross soybean symbiotic nitrogen fixation amount to be measured, complete the measuring method of field soybean nodule nitrogen fixation amount.
2. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, is characterized in that in step 1, amount of application of nitrogen fertilizer is 15kg/ha～25kg/ha.
3. the measuring method of field soybean nodule nitrogen fixation amount according to claim 2, is characterized in that in step 1, amount of application of nitrogen fertilizer is 20kg/ha.
4. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, is characterized in that in step 1, phosphate fertilizer amount is 51kg/ha, and K Amounts is 60kg/ha.
5. the measuring method of field soybean nodule nitrogen fixation amount according to claim 1, is characterized in that the soybean of destructive sampling in step 2 is the maturing stage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105481552A (en) * | 2015-12-12 | 2016-04-13 | 常州大学 | Preparation method for soybean straw slow-release nitrogen fertilizer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007846A (en) * | 2010-11-08 | 2011-04-13 | 中国科学院东北地理与农业生态研究所 | Method for detecting quantity of nitrogen fixed by soybean root nodules |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007846A (en) * | 2010-11-08 | 2011-04-13 | 中国科学院东北地理与农业生态研究所 | Method for detecting quantity of nitrogen fixed by soybean root nodules |
Non-Patent Citations (6)
| Title |
|---|
| MIAO SHU-JIE等: "Nodule Formation and Development in Soybeans (Glycine max L.)in Response to Phosphorus Supply in Solution Culture", 《PEDOSPHERE》, vol. 17, no. 1, 31 December 2007 (2007-12-31), pages 36 - 43, XP022934388, DOI: doi:10.1016/S1002-0160(07)60005-8 * |
| P. HOUNGNANDAN等: "Improved estimation of biological nitrogen fixation of soybean cultivars (Glycine max L. Merril) using 15N natural abundance technique", 《BIOL FERTIL SOILS》, vol. 45, no. 2, 31 December 2008 (2008-12-31), pages 175 - 183, XP019656416, DOI: doi:10.1007/s00374-008-0311-5 * |
| 何道文等: "利用N-15自然丰度法研究固氮植物生物固氮量", 《干旱地区农业研究》, vol. 22, no. 1, 31 March 2004 (2004-03-31), pages 132 - 137 * |
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| 石海等: "施氮对结瘤和非结瘤近等位基因大豆生长和固氮性状的影响", 《大豆科学》, vol. 31, no. 6, 31 December 2012 (2012-12-31) * |
| 陈慧等: "不同大豆品种根瘤固氮酶活性与固氮量差异研究", 《核学农报》, vol. 27, no. 3, 31 December 2013 (2013-12-31), pages 379 - 383 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105481552A (en) * | 2015-12-12 | 2016-04-13 | 常州大学 | Preparation method for soybean straw slow-release nitrogen fertilizer |
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