CN103880725A - Method for extracting astaxanthin from haematococcus pluvialis - Google Patents
Method for extracting astaxanthin from haematococcus pluvialis Download PDFInfo
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- CN103880725A CN103880725A CN201210553704.2A CN201210553704A CN103880725A CN 103880725 A CN103880725 A CN 103880725A CN 201210553704 A CN201210553704 A CN 201210553704A CN 103880725 A CN103880725 A CN 103880725A
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- astaxanthin
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Abstract
The invention discloses a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of (1) breaking walls of sporophore: taking fresh wet algae mud, adding 3 mol/L HCl with an amount of two times by mass of the fresh wet algae mud, stirring uniformly, stirring for 3 min at a temperature of 50 DEG C with a stirring speed of 180 times per minute, cooling rapidly, centrifuging and washing and taking the algae bodies; and (2) extracting astaxanthin: adding ethanol into the wall-broken sporophore with an amount of two times of the sporophore, oscillating for 5 min at a low temperature of 10 DEG C, centrifuging, recovering ethanol, repeating for 2 times, merging the recovered ethanol; concentrating at a low temperature and in vacuum; and recovering ethanol by evaporation, and thus the left material being astaxanthin. The method is simple and can be easily understood, is reasonable in operation, enables the haematococcus pluvialis to perform nutritive growth relatively well, is relatively fast in transformation from nutritive growth to non-nutritive growth, and accumulates relatively much haematococcus pluvialis, thereby increasing efficiency and stability of culture, transformation and accumulation and effectively preventing contamination of miscellaneous algae during a culture process. The manufactured astaxanthin is relatively low in cost.
Description
Technical field
The invention belongs to, be specifically related to a kind of method that extracts astaxanthin from Haematocoocus Pluvialls.
Background technology
Astaxanthin is the one of carotenoid, mainly as the natural colorant of fish.There is superpower resistance of oxidation, there is protection skin, improve immunizing power, suppress the functions such as cancer, be widely used in fishery products, makeup, the industries such as medicine.Haematocoocus Pluvialls (Haematococcus pluvialis) is under the jurisdiction of Chlorophyta, Chlorophyceae, and volvocales, Haematocoocus Pluvialls section, Haematocoocus Pluvialls belongs to.Haematocoocus Pluvialls is the current known the highest biology of content astaxanthin; it is coercing environment accumulation astaxanthin, as nutritional deficiency, and intense light irradiation etc.; the high energy of its content astaxanthin reaches the 1-4% of cell quality, is a kind of algae that has large-scale production astaxanthin potentiality.
Haematocoocus Pluvialls is a kind of unicell green alga, and accumulation astaxanthin in a large number is under given conditions the highest biology of occurring in nature accumulation natural astaxanthin ability.But Haematocoocus Pluvialls is through a series of culturing process, is finally the spore of haematococcus pluvialis of a large amount of astaxanthins of accumulation, and because astaxanthin is intracellular product, astaxanthin is all thick cell walls parcel, can not be utilized.The astaxanthin overwhelming majority of selling is in the market chemosynthesis astaxanthin, not only expensive, also may exist and pollute and potential harm, and in biological effect and security not as good as natural astaxanthin,
summary of the invention
The problems referred to above that exist in order to overcome prior art field, the object of the invention is to, a kind of method that extracts astaxanthin from Haematocoocus Pluvialls is provided, and solve commercially available astaxanthin and be chemosynthesis, expensive problem.
The method that extracts astaxanthin from Haematocoocus Pluvialls provided by the invention, it comprises following concrete steps:
(1) sporophyte broken wall is got: get fresh wet algae mud, add the HCl of 2 times of mass ratio 3mol/L, stir, and 50 ℃, stir 3min, stirring velocity per minute 180 times, more cooling rapidly, after centrifuge washing, get frond;
(2) extraction astaxanthin: in broken sporophyte, add 2 times of ethanol, 10 ℃ of low temperature, concussion 5min, centrifugal, reclaim ethanol, 2 times repeatedly, the ethanol of secondary merges, and cryogenic vacuum is concentrated, and ethanol volatilization is reclaimed, and the material staying is astaxanthin.
The method that extracts astaxanthin from Haematocoocus Pluvialls provided by the invention, its beneficial effect is, the method is easily understood, manipulation rationally, makes nourishing and growing better of Haematocoocus Pluvialls, nourishes and grows and transforms comparatively fast to non-nourishing and growing, accumulation astaxanthin is more, thereby improved efficiency and the stability of cultivation, conversion, accumulation, also effectively prevented the pollution of assorted algae in culturing process, the astaxanthin cost of making is low.
Embodiment
Below in conjunction with embodiment, to the method that extracts astaxanthin from Haematocoocus Pluvialls provided by the invention, be described in detail.
Embodiment
The method that extracts astaxanthin from Haematocoocus Pluvialls of the present embodiment, it comprises following concrete steps:
(1) sporophyte broken wall is got: get fresh wet algae mud, add the HCl of 2 times of mass ratio 3mol/L, stir, and 50 ℃, stir 3min, stirring velocity per minute 180 times, more cooling rapidly, after centrifuge washing, get frond;
(2) extraction astaxanthin: in broken sporophyte, add 2 times of ethanol, 10 ℃ of low temperature, concussion 5min, centrifugal, reclaim ethanol, 2 times repeatedly, the ethanol of secondary merges, and cryogenic vacuum is concentrated, and ethanol volatilization is reclaimed, and the material staying is astaxanthin.
Due to spore of haematococcus pluvialis wall thickness and solid, and sporophyte is very tiny, bring many difficulty to broken wall, the present invention adopts acid heat method, get fresh wet algae mud, the HCl that adds 2 times of mass ratio 3mol/L, stirs, 50 ℃, stir 3min, stirring velocity per minute 180 times, rear cooling rapidly, after centrifuge washing, get frond; Astaxanthin is that fat-soluble, conventional organic solvent all can be used as organic solvent, as methyl alcohol, ethanol, sherwood oil, methylene dichloride, acetone, ethyl acetate etc., consider, and ethanol net effect is better, in broken sporophyte, add 2 times of ethanol, 10 ℃ of low temperature, concussion 5min, centrifugal, reclaim ethanol, 2 times repeatedly, the ethanol of secondary merges, cryogenic vacuum is concentrated, and ethanol volatilization is reclaimed, and the material staying is astaxanthin.
Claims (1)
1. a method that extracts astaxanthin from Haematocoocus Pluvialls, is characterized in that: it comprises following concrete steps:
One, sporophyte broken wall is got: get fresh wet algae mud, add the HCl of 2 times of mass ratio 3mol/L, stir, and 50 ℃, stir 3min, stirring velocity per minute 180 times, more cooling rapidly, after centrifuge washing, get frond;
Two, extraction astaxanthin: in broken sporophyte, add 2 times of ethanol, 10 ℃ of low temperature, concussion 5min, centrifugal, reclaim ethanol, 2 times repeatedly, the ethanol of secondary merges, and cryogenic vacuum is concentrated, and ethanol volatilization is reclaimed, and the material staying is astaxanthin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210553704.2A CN103880725A (en) | 2012-12-19 | 2012-12-19 | Method for extracting astaxanthin from haematococcus pluvialis |
Applications Claiming Priority (1)
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| CN201210553704.2A CN103880725A (en) | 2012-12-19 | 2012-12-19 | Method for extracting astaxanthin from haematococcus pluvialis |
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| CN103880725A true CN103880725A (en) | 2014-06-25 |
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| CN201210553704.2A Pending CN103880725A (en) | 2012-12-19 | 2012-12-19 | Method for extracting astaxanthin from haematococcus pluvialis |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104557651A (en) * | 2014-12-31 | 2015-04-29 | 云南民族大学 | Method for extracting astaxanthin from haematococcus pluvialis by double-aqueous-phase coupled wall breaking technology |
| CN104705654A (en) * | 2015-04-02 | 2015-06-17 | 徐权汉 | Preparation method for astaxanthin microcapsules |
| CN104961668A (en) * | 2015-06-23 | 2015-10-07 | 晨光生物科技集团股份有限公司 | Method for quickly extracting astaxanthin from haematococcus pluvialis |
| CN105394749A (en) * | 2015-10-23 | 2016-03-16 | 武汉龙族药号生物医药科技有限公司 | Formula, preparation method and application of astaxanthin-containing health food |
| CN110724083A (en) * | 2019-10-29 | 2020-01-24 | 四川轻化工大学 | Method for efficiently extracting astaxanthin from haematococcus pluvialis mud |
| CN111875528A (en) * | 2020-08-30 | 2020-11-03 | 云南爱尔康生物技术有限公司 | Method for preparing astaxanthin oil from haematococcus pluvialis |
-
2012
- 2012-12-19 CN CN201210553704.2A patent/CN103880725A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104557651A (en) * | 2014-12-31 | 2015-04-29 | 云南民族大学 | Method for extracting astaxanthin from haematococcus pluvialis by double-aqueous-phase coupled wall breaking technology |
| CN104705654A (en) * | 2015-04-02 | 2015-06-17 | 徐权汉 | Preparation method for astaxanthin microcapsules |
| CN104961668A (en) * | 2015-06-23 | 2015-10-07 | 晨光生物科技集团股份有限公司 | Method for quickly extracting astaxanthin from haematococcus pluvialis |
| CN105394749A (en) * | 2015-10-23 | 2016-03-16 | 武汉龙族药号生物医药科技有限公司 | Formula, preparation method and application of astaxanthin-containing health food |
| CN105394749B (en) * | 2015-10-23 | 2018-03-20 | 武汉龙族药号生物医药科技有限公司 | A kind of Ricipe for health care food and preparation method and application containing astaxanthin |
| CN110724083A (en) * | 2019-10-29 | 2020-01-24 | 四川轻化工大学 | Method for efficiently extracting astaxanthin from haematococcus pluvialis mud |
| CN110724083B (en) * | 2019-10-29 | 2021-06-01 | 四川轻化工大学 | Method for efficiently extracting astaxanthin from haematococcus pluvialis mud |
| CN111875528A (en) * | 2020-08-30 | 2020-11-03 | 云南爱尔康生物技术有限公司 | Method for preparing astaxanthin oil from haematococcus pluvialis |
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Application publication date: 20140625 |