CN104726340A - Dunaliella collection method - Google Patents
Dunaliella collection method Download PDFInfo
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- CN104726340A CN104726340A CN201510152394.7A CN201510152394A CN104726340A CN 104726340 A CN104726340 A CN 104726340A CN 201510152394 A CN201510152394 A CN 201510152394A CN 104726340 A CN104726340 A CN 104726340A
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- salt algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention belongs to the technical field of alga organism collection, and relates to a Dunaliella collection method. The method comprises the following steps: pumping a Dunaliella culture solution into an upper cylinder via a feed port, starting an upper disk centrifuge and a stirrer, adding a flocculant, cooling the Dunaliella culture solution to inactivate the algae to form an alga floccule which is centralized to a middle cylinder and sinks into the a lower cylinder, opening a drainage port valve to discharge the alga floccule supernate via a drainage port, carrying out initial separation on the Dunaliella and culture solution, gradually concentrating the Dunaliella floccule in the lower cylinder, starting a lower disk centrifuge until the Dunaliella is thoroughly separated from the culture solution, opening a discharge port valve to discharge Dunaliella slurry via a discharge port, diluting with distilled water, carrying out centrifugal dehydration, and cleaning to purify and elute all the salts and flocculant on the Dunaliella surface, thereby implementing Dunaliella collection. The method has the advantages of simple collection technique, high product activity, stable performance, reliable principle, high safety, environment friendliness and high utilization ratio, and is free from the influence of the external environment.
Description
Technical field:
The invention belongs to algae bio harvesting technique field, relate to a kind of salt algae collecting method, disposablely can not to gather on a large scale the problem of salt algae to solve prior art, manufacture field for algal product.
Background technology:
Salt algae is the single celled eukaryotic algae of a kind of existence in the dense salt lake of height, be under the jurisdiction of Chlorophyta Chlorophyceae volvocales Yan Zao section Halophila, for green single-cell algae, body is small, acellular wall, there is the bag quilt that glycoprotein is formed, the unique peculiar life can survived in high density salt solution found at present, owing to being rich in uniqueness and abundant bioelement, be described as " propulsion source of cell " by World Science circle, " protective material of life ", salt algae is rich in β-carotene, artificial culture can therefrom extract glycerine or carotene, also fish can be done, shrimp, the bait of the young such as shellfish, can prevent and assisting therapy coronary heart disease, cardiovascular and cerebrovascular diseases, diabetes, cancer, hepatic diseases, stomach common disease, stomatocace, cataract, xerophthalmia at medicine and hygiene fields, alleviate chemotherapy adverse effect, improve eyesight, improve immunizing power, anti-oxidant, delay senility, due to the acellular wall of salt algae, add in animal-feed, can promote that feed conversion rate improves the speed of growth of animal, reduce disease, exploitation " green feed " is significant to raising animal food prods quality, the economic worth that salt algae is huge and unique advantage have become the object of large scale culturing, a lot of countries and regions are all in cultivation and exploitation salt algae, comprise Australia, Japan, Israel, the U.S. etc., the cultivation of salt algae has also been carried out in Hainan of China and the Inner Mongol, due to Halophila planktonic organism, build microcell is long 14 ~ 22 μm, wide 3 ~ 14 μm, acellular wall, and formalness not fixed random changes in environmental conditions and changing, shows the variforms such as pyriform, ellipse, long-neck doleiform, Chinese patent 201310026310.6 discloses the collecting method of a kind of salt algae on (January 23 2013 applying date): 1) be cultured to the later stage in salt algae, just can gather during algae cell density reaches (1 ~ 10) × 106/more than mL, 2) in algae liquid, add flocculation agent mother liquor, stir evenly, leave standstill, rear removing supernatant, collect and obtain algae mud, calculate flocculation efficiency, employing inorganic flocculating agent carrys out the salt algae in the nutrient solution of efficient flocculating enrichment high salinity.Flocculant usage is determined according to the temperature of algae liquid, salinity, pH, cell concn, and minimum amount is no less than 10mg/L, the highest 2000mg/L that is no more than; It cannot realize being separated completely of salt algae and nutrient solution, and once the amount of gathering is low, can not form gathering of mass-producing, affect the production technique of algal product; Therefore research and develop a kind of salt algae to be separated completely from nutrient solution, the single amount of gathering reaches the collecting method of more than a ton, has good application prospect and social value.
Summary of the invention:
The object of the invention is to the shortcoming overcoming prior art existence, seeking design one can separate salt algae completely from nutrient solution, the amount of gathering can be made once again to reach the salt algae collecting method of more than a ton, be applied to algal product and manufacture field.
To achieve these goals, salt algae collecting method of the present invention is implemented in salt algae recovering device, and its process is: inject water coolant to interlayer from water-in, water coolant recycles in interlayer, salt algae culturing liquid is pumped in the cylindrical shell of top by opening for feed, start top disk centrifugal separator and agitator, add flocculation agent, cool gradually under the effect of salt algae culturing liquid water coolant in interlayer, frond loses movable function, under the stirring and flocculation agent acting in conjunction of agitator, form frond throw out, and concentrate gradually to middle cylinder, slowly sink down in lower cylinder, open sewage draining exit valve, frond throw out supernatant liquor is discharged from sewage draining exit, salt algae and nutrient solution initial gross separation, salt algae throw out in lower cylinder concentrates gradually, start bottom disk centrifugal separator, algae is thoroughly separated salt from nutrient solution, open discharge port valve, salt algae slurry is the distilled water diluting that salt algae starches 15 times by weight again after discharging from discharge port, centrifuge dehydration, clean three times, the salt on salt algae surface and flocculation agent is made all to purify wash-out, obtain pure salt algae product, realize gathering of salt algae.
The agent structure of the salt algae recovering device that the present invention relates to comprises top cylindrical shell, interlayer, opening for feed, discharge port, top disk centrifugal separator, agitator, sewage draining exit, sewage draining exit valve, lower cylinder, bottom disk centrifugal separator, water-in, water outlet, discharge port valve and middle cylinder; Top cylindrical shell is diameter 90cm, height 1.5m's is cylindrical, cylindrical shell periphery, top is shaped with interlayer, and interlayer top is shaped with water-in, and interlayer bottom is shaped with water outlet, aqueous liquids is housed in interlayer be used for cooling top cylindrical shell, the top of top cylindrical shell is shaped with opening for feed, and top butterfly centrifugal machine is fixedly connected with the agitator of fixing top inner barrel through the top of top cylindrical shell, and middle cylinder is inverted trapezoidal structure, its top is shaped with sewage draining exit, is fixedly shaped with sewage draining exit valve in sewage draining exit; The upper end of middle cylinder seals with the lower end of top cylindrical shell and docks, and the lower end of middle cylinder seals with the upper end of lower cylinder and docks, the diameter 40cm of the lower cylinder of cylindrical structure, is highly 60cm; The inside of lower cylinder is shaped with bottom disk centrifugal separator, and lower cylinder bottom is shaped with discharge port, is fixedly shaped with discharge port valve in discharge port; The inner chamber of top cylindrical shell, middle cylinder and lower cylinder is by the corresponding salt algae recovering device being fixedly connected to form upper coarse and lower fine integral structure of sealing, and the total height of salt algae recovering device is 2-2.5m; When recovering device is installed, its discharge port is overhead highly greater than 40cm.
The flocculation agent that the present invention relates to is bodied ferric sulfate, and be nontoxic inorganic polymer, the addition in salt algae culturing liquid is 20ppm, utilizes the bridging action of bodied ferric sulfate, makes the frond losing movable function form flocs unit, accelerates frond and sinks.
The supernatant liquor that in the present invention, sewage draining exit is discharged is recycled after overnutrition adjustment; The salt algae recovering device that the present invention relates to once can concentrating and separating salt algae culturing liquid more than one ton.
The present invention compared with prior art, salt algae can be separated completely from nutrient solution, the amount of gathering can be made once again to reach more than one ton, the salt algae after separation still keeps activity character, nutrient solution after separation is recoverable after nutrition adjustment, its recovery process is simple, and Product Activity is high, stable performance, be not affected by the external environment, principle is reliable, and safety and environmental protection, utilization ratio is high.
Accompanying drawing illustrates:
Fig. 1 is the recovering device agent structure principle schematic that the present invention relates to.
Embodiment:
Below in conjunction with accompanying drawing, by embodiment, the invention will be further described.
Embodiment 1:
The salt algae collecting method of the present embodiment is being implemented in salt algae recovering device, and its process is: inject water coolant to interlayer 2 from water-in 11, water coolant recycles in interlayer 2, salt algae culturing liquid is pumped in top cylindrical shell 1 by opening for feed 3, start top disk centrifugal separator 5 and agitator 6, add flocculation agent, cool gradually under the effect of salt algae culturing liquid water coolant in interlayer 2, frond loses movable function, under the stirring and flocculation agent acting in conjunction of agitator 6, form frond throw out, and concentrate gradually to middle cylinder 14, slowly sink down in lower cylinder 9, open sewage draining exit valve 8, frond throw out supernatant liquor is discharged from sewage draining exit 7, salt algae and nutrient solution initial gross separation, salt algae throw out in lower cylinder 9 concentrates gradually, start bottom disk centrifugal separator 10, algae is thoroughly separated salt from nutrient solution, open discharge port valve 13, salt algae slurry is the distilled water diluting that salt algae starches 15 times by weight again after discharging from discharge port 4, centrifuge dehydration, clean three times, the salt on salt algae surface and flocculation agent is made all to purify wash-out, obtain pure salt algae product, realize gathering of salt algae.
The agent structure of the salt algae recovering device that the present embodiment relates to comprises top cylindrical shell 1, interlayer 2, opening for feed 3, discharge port 4, top disk centrifugal separator 5, agitator 6, sewage draining exit 7, sewage draining exit valve 8, lower cylinder 9, bottom disk centrifugal separator 10, water-in 11, water outlet 12, discharge port valve 13 and middle cylinder 14; Top cylindrical shell 1 is diameter 90cm, height 1.5m's is cylindrical, top cylindrical shell 1 periphery is shaped with interlayer 2, interlayer 2 top is shaped with water-in 11, interlayer 2 bottom is shaped with water outlet 12, aqueous liquids is housed in interlayer 2 be used for cooling top cylindrical shell 1, the top of top cylindrical shell 1 is shaped with opening for feed 3, top butterfly centrifugal machine 5 is fixedly connected with the agitator 6 of cylindrical shell 1 inside, fixing top through the top of top cylindrical shell 1, middle cylinder 14 is inverted trapezoidal structure, its top is shaped with sewage draining exit 7, is fixedly shaped with sewage draining exit valve 8 in sewage draining exit 7; The upper end of middle cylinder 14 seals with the lower end of top cylindrical shell 1 and docks, and the lower end of middle cylinder 14 seals with the upper end of lower cylinder 9 and docks, the diameter 40cm of the lower cylinder 9 of cylindrical structure, is highly 60cm; The inside of lower cylinder 9 is shaped with bottom disk centrifugal separator 10, and lower cylinder 9 bottom is shaped with discharge port 4, is fixedly shaped with discharge port valve 13 in discharge port 4; The inner chamber of top cylindrical shell 1, middle cylinder 14 and lower cylinder 9 is by the corresponding salt algae recovering device being fixedly connected to form upper coarse and lower fine integral structure of sealing, and the total height of salt algae recovering device is 2-2.5m; When recovering device is installed, its discharge port 4 is overhead highly greater than 40cm.
The flocculation agent that the present embodiment relates to is bodied ferric sulfate, and be nontoxic inorganic polymer, the addition in salt algae culturing liquid is 20ppm, utilizes the bridging action of bodied ferric sulfate, makes the frond losing movable function form flocs unit, accelerates frond and sinks.
The supernatant liquor that in the present embodiment, sewage draining exit 7 is discharged is recycled after overnutrition adjustment; The salt algae recovering device that the present embodiment relates to once can concentrating and separating salt algae culturing liquid more than one ton.
Claims (4)
1. a salt algae collecting method, it is characterized in that salt algae collecting method is implemented in salt algae recovering device, its process is: inject water coolant to interlayer from water-in, water coolant recycles in interlayer, salt algae culturing liquid is pumped in the cylindrical shell of top by opening for feed, start top disk centrifugal separator and stirring, add flocculation agent, cool gradually under the effect of salt algae culturing liquid water coolant in interlayer, frond loses movable function, under the stirring and flocculation agent acting in conjunction of agitator, form frond throw out, and concentrate gradually to middle cylinder, slowly sink down in lower cylinder, open sewage draining exit valve, frond throw out supernatant liquor is discharged from sewage draining exit, salt algae and nutrient solution initial gross separation, salt algae throw out in lower cylinder concentrates gradually, start bottom disk centrifugal separator, algae is thoroughly separated salt from nutrient solution, open discharge port valve, salt algae slurry is the distilled water diluting that salt algae starches 15 times by weight again after discharging from discharge port, centrifuge dehydration, clean three times, the salt on salt algae surface and flocculation agent is made all to purify wash-out, obtain pure salt algae product, realize gathering of salt algae.
2. salt algae collecting method according to claim 1, is characterized in that the agent structure of described salt algae recovering device comprises top cylindrical shell, interlayer, opening for feed, discharge port, top disk centrifugal separator, agitator, sewage draining exit, sewage draining exit valve, lower cylinder, bottom disk centrifugal separator, water-in, water outlet, discharge port valve and middle cylinder; Top cylindrical shell is diameter 90cm, height 1.5m's is cylindrical, cylindrical shell periphery, top is shaped with interlayer, and interlayer top is shaped with water-in, and interlayer bottom is shaped with water outlet, aqueous liquids is housed in interlayer be used for cooling top cylindrical shell, the top of top cylindrical shell is shaped with opening for feed, and top butterfly centrifugal machine is fixedly connected with the agitator of fixing top inner barrel through the top of top cylindrical shell, and middle cylinder is inverted trapezoidal structure, its top is shaped with sewage draining exit, is fixedly shaped with sewage draining exit valve in sewage draining exit; The upper end of middle cylinder seals with the lower end of top cylindrical shell and docks, and the lower end of middle cylinder seals with the upper end of lower cylinder and docks, the diameter 40cm of the lower cylinder of cylindrical structure, is highly 60cm; The inside of lower cylinder is shaped with bottom disk centrifugal separator, and lower cylinder bottom is shaped with discharge port, is fixedly shaped with discharge port valve in discharge port; The inner chamber of top cylindrical shell, middle cylinder and lower cylinder is by the corresponding salt algae recovering device being fixedly connected to form upper coarse and lower fine integral structure of sealing, and the total height of salt algae recovering device is 2-2.5m; When recovering device is installed, its discharge port is overhead highly greater than 40cm.
3. salt algae collecting method according to claim 1, it is characterized in that described flocculation agent is bodied ferric sulfate, it is nontoxic inorganic polymer, addition in salt algae culturing liquid is 20ppm, utilize the bridging action of bodied ferric sulfate, make the frond losing movable function form flocs unit, accelerate frond and sink.
4. salt algae collecting method according to claim 1, is characterized in that the supernatant liquor that described sewage draining exit is discharged is recycled after overnutrition adjustment; Described salt algae recovering device once can concentrating and separating salt algae culturing liquid more than one ton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510152394.7A CN104726340A (en) | 2015-04-28 | 2015-04-28 | Dunaliella collection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510152394.7A CN104726340A (en) | 2015-04-28 | 2015-04-28 | Dunaliella collection method |
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| CN104726340A true CN104726340A (en) | 2015-06-24 |
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| CN201510152394.7A Pending CN104726340A (en) | 2015-04-28 | 2015-04-28 | Dunaliella collection method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255737A (en) * | 2015-10-27 | 2016-01-20 | 卢俊 | Desalination sedimentation method for reducing phaeophytin in dunaliella salina |
| CN106076662A (en) * | 2016-06-29 | 2016-11-09 | 哈尔滨华藻生物科技开发有限公司 | A kind of spirulina centrifugal separating device |
| CN107435027A (en) * | 2017-01-09 | 2017-12-05 | 内蒙古超越健生物科技有限责任公司 | A kind of collecting method of Dunaliella salina |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204455A (en) * | 2011-06-10 | 2011-10-05 | 薛命雄 | Spirulina flocculating and harvesting device and harvesting method |
| CN104711165A (en) * | 2015-04-02 | 2015-06-17 | 徐权汉 | Dunaliella salina collecting device |
| CN204529841U (en) * | 2015-04-02 | 2015-08-05 | 徐权汉 | A kind of salt algae recovering device |
-
2015
- 2015-04-28 CN CN201510152394.7A patent/CN104726340A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204455A (en) * | 2011-06-10 | 2011-10-05 | 薛命雄 | Spirulina flocculating and harvesting device and harvesting method |
| CN104711165A (en) * | 2015-04-02 | 2015-06-17 | 徐权汉 | Dunaliella salina collecting device |
| CN204529841U (en) * | 2015-04-02 | 2015-08-05 | 徐权汉 | A kind of salt algae recovering device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255737A (en) * | 2015-10-27 | 2016-01-20 | 卢俊 | Desalination sedimentation method for reducing phaeophytin in dunaliella salina |
| CN105255737B (en) * | 2015-10-27 | 2018-03-16 | 卢俊 | A kind of desalination sedimentation method for reducing pheophytin in salt algae |
| CN106076662A (en) * | 2016-06-29 | 2016-11-09 | 哈尔滨华藻生物科技开发有限公司 | A kind of spirulina centrifugal separating device |
| CN107435027A (en) * | 2017-01-09 | 2017-12-05 | 内蒙古超越健生物科技有限责任公司 | A kind of collecting method of Dunaliella salina |
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Application publication date: 20150624 |