CN103877172A - Compound Chinese herbal medicine capable of treating putrid-skin disease of holothurian and its production method - Google Patents

Abstract

The invention discloses a compound Chinese herbal medicine capable of treating putrid-skin disease of holothurian and its production method, the compound Chinese herbal medicine capable of treating putrid-skin disease of holothurian comprises the following eight Chinese herbal medicines of andrographis paniculata, ligusticum wallichii, radix astragali, rheum officinale, Chinese gall, bighead atractylodes rhizome, licorice and honeysuckle flower with ratio of 25: 12: 19: 12: 10: 16: 12: 18, and the production method for the compound Chinese herbal medicine comprises the following steps: 1)decocting and extracting for first time; 2) decocting for second time; 3) performing double-effect concentration; and 4)spray drying. The production method is simple and easy to understand, bacteriostasis and sterilization, antiviral action and insecticide can not be directly inhibited, drug resistance is not generated, the body immunity can be adjusted and increased, and the disease-resistant capability of the holothurians body is increased; during a culture process of the holothurians, the disease prevention medication of the compound Chinese herbal medicine has no adverse influence to the holothurians growth, the compound Chinese herbal medicine has obvious growth promotion effect to the holothurians, and the survival rate for culturing holothurians can be increased.

Description

Compound Chinese herb for the treatment of Stichopus japonicus skin fester disease and preparation method thereof

 

Technical field

The invention belongs to the Chinese herbal medicine for aquatic animal, be specifically related to a kind of compound Chinese herb for the treatment of Stichopus japonicus skin fester disease and preparation method thereof.

 

Background technology

Stichopus japonicus rich in proteins and mucopolysaccharide, nutrition and medical value are high.In recent years, along with the increase of people to Stichopus japonicus demand, holothruian cultures fast development, become aquaculture pillar industry, but, the disease problem being on the rise in holothruian cultures process, but produced, Radix Morinae Bulleyanae skin fester disease involves wide, infectiousness is strong, and mortality rate is high, is one of important disease of culturing stichopus japonicus in recent years.Face the Radix Morinae Bulleyanae skin fester disease that takes place frequently in holothruian cultures, culturist drops into a large amount of medicines blindly in the hope of controlling the development of disease and spreading, as used chemosterilant, antibiotic and other aquaculture Chinese herbal and crude drugs preparations etc. of a multitude of names.Chemosterilant, just may make host animal impaired or lethal killing before cause of disease conventionally; Antibiotic is only effective to bacterial disease, very little to viral disease effect, and has larger toxic and side effects, and extended residual, in organism, finally can work the mischief to the crowd of this organism of ingesting.

 

Summary of the invention

The problems referred to above that exist in order to overcome prior art field, the object of the invention is to, a kind of compound Chinese herb for the treatment of Stichopus japonicus skin fester disease and preparation method thereof is provided, solve chemotherapy sea cucumber diseases such as using antibiotic, chemical drugs can residue in organism for a long time, the problem that finally can work the mischief to the crowd of this organism of ingesting.

Compound Chinese herb for the treatment of Stichopus japonicus skin fester disease provided by the invention and preparation method thereof, the compound Chinese herb of described treatment Stichopus japonicus skin fester disease comprises 8 taste Chinese herbal medicine, and described 8 taste Chinese herbal medicine ratios are Herba Andrographis: Rhizoma Chuanxiong: the Radix Astragali: Radix Et Rhizoma Rhei: Galla Chinensis: the Rhizoma Atractylodis Macrocephalae: Radix Glycyrrhizae: Flos Lonicerae=25:12: 19:12:10:16:12:18.

The manufacture method of the compound Chinese herb for the treatment of Stichopus japonicus skin fester disease, it comprises following concrete steps:

(1) decoct and extract for the first time: after procedure for preparation completes, first multifunctional extracting pot cleanout door is closed, extraction pot top dog-house is opened, in proportion 8 taste Chinese herbal medicine are dropped in extraction pot, open extraction pot inlet valve, to the drinking water that is metered into 8 times of amounts of middle dose in extraction pot, close inlet valve and dog-house; Open steam valve, be heated to 40 ℃, warm macerating, after 30 minutes, continues to be heated to boiling; In decoction process, observe at any time steam gage, thermometer and extraction pot Pressure gauge numerical value, and recording start boiling time, temperature, now jacket steam pressure keeps 0.02Mpa, decocts temperature and is controlled at 100 ℃ of left and right, in tank, keep fluidized state, decoct 2 hours; Decoction process should constantly be observed decoction situation, adjust steam pressure at any time from visor; After decocting 2 hours, steam off valve, opens the fluid valve of extraction pot, and decocting liquid is crossed to 200 order filters, squeezes in fluid reservoir, and drawing liquid is complete, hangs Status Flag on fluid reservoir, indicates decoction liquor title, lot number, quantity, date, operator etc.;

(2) decoct for the second time: open extraction pot inlet valve, be metered into 6 times of amount drinking waters, open steam valve and repeat the above operation that decocts, decocting liquid is crossed to 200 order filters after decocting 2 hours, squeeze in fluid reservoir, medicinal liquid total amount must not be lower than 3500L, and operating process has special messenger to carry out process monitoring, and makes a record;

(3) economic benefits and social benefits are concentrated: first the vacuum of dual-effect concentrator, cooling water valve are opened, by medicinal liquid suction dual-effect concentrator, open steam valve and carry out concentrating under reduced pressure, evaporating temperature: 75-85 ℃ of an effect, two effect 60-70 ℃, vacuum: effect-0.03～-0.04Mpa, two effect-0.06～-0.07Mpa, in concentration process, to notice at any time that each table numerical control is in safety range, and perform record; Concentrate while closely end, open the feed opening graduated cylinder of heater and collect concentrated solution, with gravity Baume instrumentation proportion, as inadequate in proportion, should continue to concentrate, until concentrated solution relative density reaches at 1.05 o'clock, i.e. 60 ℃ of surveys, close vacuum valve, steam valve and cooling water valve, open the atmospheric valve of vaporization chamber, put cream;

(4) spraying is dry squeezes into concentrated solution in spray dryer and sprays and be dried, and obtains the former medicine of extract, adds carrier 20%, synergist 5% simultaneously.

Compound Chinese herb for the treatment of Stichopus japonicus skin fester disease provided by the invention and preparation method thereof, its beneficial effect is, the method is easily understood, not only directly bacteriostasis and sterilization, antiviral, parasite killing, do not produce drug resistance, and can regulate and improve immunity of organisms, strengthen the resistance against diseases of Stichopus japonicus body; Not only Stichopus japonicus is grown without any harmful effect as prevention of damage by disease medication in holothruian cultures process, oppose that mutually Stichopus japonicus has obvious growth promoting function, can improve the survival rate of culturing sea cucumbers; Residual without toxic medicament in Stichopus japonicus body, food without any side effects to human body.

 

The specific embodiment

Below in conjunction with embodiment, compound Chinese herb to treatment Stichopus japonicus skin fester disease provided by the invention and preparation method thereof, is described in detail.

Embodiment

Compound Chinese herb of the treatment Stichopus japonicus skin fester disease of the present embodiment and preparation method thereof, the compound Chinese herb of described treatment Stichopus japonicus skin fester disease comprises 8 taste Chinese herbal medicine, and described 8 taste Chinese herbal medicine ratios are Herba Andrographis: Rhizoma Chuanxiong: the Radix Astragali: Radix Et Rhizoma Rhei: Galla Chinensis: the Rhizoma Atractylodis Macrocephalae: Radix Glycyrrhizae: Flos Lonicerae=25:12: 19:12:10:16:12:18.

The manufacture method of the compound Chinese herb for the treatment of Stichopus japonicus skin fester disease, it comprises following concrete steps:

(1) decoct and extract for the first time: after procedure for preparation completes, first multifunctional extracting pot cleanout door is closed, extraction pot top dog-house is opened, in proportion 8 taste Chinese herbal medicine are dropped in extraction pot, open extraction pot inlet valve, to the drinking water that is metered into 8 times of amounts of middle dose in extraction pot, close inlet valve and dog-house; Open steam valve, be heated to 40 ℃, warm macerating, after 30 minutes, continues to be heated to boiling; In decoction process, observe at any time steam gage, thermometer and extraction pot Pressure gauge numerical value, and recording start boiling time, temperature, now jacket steam pressure keeps 0.02Mpa, decocts temperature and is controlled at 100 ℃ of left and right, in tank, keep fluidized state, decoct 2 hours; Decoction process should constantly be observed decoction situation, adjust steam pressure at any time from visor; After decocting 2 hours, steam off valve, opens the fluid valve of extraction pot, and decocting liquid is crossed to 200 order filters, squeezes in fluid reservoir, and drawing liquid is complete, hangs Status Flag on fluid reservoir, indicates decoction liquor title, lot number, quantity, date, operator etc.;

(2) decoct for the second time: open extraction pot inlet valve, be metered into 6 times of amount drinking waters, open steam valve and repeat the above operation that decocts, decocting liquid is crossed to 200 order filters after decocting 2 hours, squeeze in fluid reservoir, medicinal liquid total amount must not be lower than 3500L, and operating process has special messenger to carry out process monitoring, and makes a record;

(3) economic benefits and social benefits are concentrated: first the vacuum of dual-effect concentrator, cooling water valve are opened, by medicinal liquid suction dual-effect concentrator, open steam valve and carry out concentrating under reduced pressure, evaporating temperature: 75-85 ℃ of an effect, two effect 60-70 ℃, vacuum: effect-0.03～-0.04Mpa, two effect-0.06～-0.07Mpa, in concentration process, to notice at any time that each table numerical control is in safety range, and perform record; Concentrate while closely end, open the feed opening graduated cylinder of heater and collect concentrated solution, with gravity Baume instrumentation proportion, as inadequate in proportion, should continue to concentrate, until concentrated solution relative density reaches at 1.05 o'clock, i.e. 60 ℃ of surveys, close vacuum valve, steam valve and cooling water valve, open the atmospheric valve of vaporization chamber, put cream;

(4) spraying is dry squeezes into concentrated solution in spray dryer and sprays and be dried, and obtains the former medicine of extract, adds carrier 20%, synergist 5% simultaneously.

Be inoculated in respectively ordinary nutrient agar slant culture medium from ill Stichopus japonicus affected area separation and purification bacterial strain, after 28 ℃ of constant temperature culture 18-24h, wash down and make hemorrhagic disease bacteria suspension and select 180 of healthy Stichopus japonicuss with physiological saline solution, lumbar injection bacterium liquid 0.25ml, it is 25 ℃ that all test fishes are fed respectively in water temperature by group, in each aquarium of pH7.2, each cylinder use gas stone inflation, observe morbidity and the death condition of each group of fish every day, within second day, starting has death, observe and find that majority is infected, divide six groups, every group 20, normally throw something and feed for 3 groups, other 3 groups with made compound Chinese herb 20PPM dipping, and with compound Chinese herb 30g/kg feedstuff, mix to raise and throw something and feed.Result does not have dipping and three groups of the Chinese herbal feed of throwing something and feeding average mortalities after five days reach 63%, and other three groups of average mortalities are respectively 27%, are starkly lower than matched group.

Claims (2)

1. treat the compound Chinese herb of Stichopus japonicus skin fester disease for one kind, it is characterized in that: the compound Chinese herb of described treatment Stichopus japonicus skin fester disease comprises 8 taste Chinese herbal medicine, and described 8 taste Chinese herbal medicine ratios are Herba Andrographis: Rhizoma Chuanxiong: the Radix Astragali: Radix Et Rhizoma Rhei: Galla Chinensis: the Rhizoma Atractylodis Macrocephalae: Radix Glycyrrhizae: Flos Lonicerae=25:12: 19:12:10:16:12:18.

2. the manufacture method of the compound Chinese herb for the treatment of Stichopus japonicus skin fester disease according to claim 1, is characterized in that: it comprises following concrete steps:

(1) decoct and extract for the first time: after procedure for preparation completes, first multifunctional extracting pot cleanout door is closed, extraction pot top dog-house is opened, in proportion 8 taste Chinese herbal medicine are dropped in extraction pot, open extraction pot inlet valve, to the drinking water that is metered into 8 times of amounts of middle dose in extraction pot, close inlet valve and dog-house; Open steam valve, be heated to 40 ℃, warm macerating, after 30 minutes, continues to be heated to boiling; In decoction process, observe at any time steam gage, thermometer and extraction pot Pressure gauge numerical value, and recording start boiling time, temperature, now jacket steam pressure keeps 0.02Mpa, decocts temperature and is controlled at 100 ℃ of left and right, in tank, keep fluidized state, decoct 2 hours; Decoction process should constantly be observed decoction situation, adjust steam pressure at any time from visor; After decocting 2 hours, steam off valve, opens the fluid valve of extraction pot, and decocting liquid is crossed to 200 order filters, squeezes in fluid reservoir, and drawing liquid is complete, hangs Status Flag on fluid reservoir, indicates decoction liquor title, lot number, quantity, date, operator etc.;

(2) decoct for the second time: open extraction pot inlet valve, be metered into 6 times of amount drinking waters, open steam valve and repeat the above operation that decocts, decocting liquid is crossed to 200 order filters after decocting 2 hours, squeeze in fluid reservoir, medicinal liquid total amount must not be lower than 3500L, and operating process has special messenger to carry out process monitoring, and makes a record;

(3) economic benefits and social benefits are concentrated: first the vacuum of dual-effect concentrator, cooling water valve are opened, by medicinal liquid suction dual-effect concentrator, open steam valve and carry out concentrating under reduced pressure, evaporating temperature: 75-85 ℃ of an effect, two effect 60-70 ℃, vacuum: effect-0.03～-0.04Mpa, two effect-0.06～-0.07Mpa, in concentration process, to notice at any time that each table numerical control is in safety range, and perform record; Concentrate while closely end, open the feed opening graduated cylinder of heater and collect concentrated solution, with gravity Baume instrumentation proportion, as inadequate in proportion, should continue to concentrate, until concentrated solution relative density reaches at 1.05 o'clock, i.e. 60 ℃ of surveys, close vacuum valve, steam valve and cooling water valve, open the atmospheric valve of vaporization chamber, put cream;

(4) spraying is dry squeezes into concentrated solution in spray dryer and sprays and be dried, and obtains the former medicine of extract, adds carrier 20%, synergist 5% simultaneously.