CN103859024A - Production method for fermented milk through symbiosis of bifidobacterium and yeast - Google Patents
Production method for fermented milk through symbiosis of bifidobacterium and yeast Download PDFInfo
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- CN103859024A CN103859024A CN201210542955.0A CN201210542955A CN103859024A CN 103859024 A CN103859024 A CN 103859024A CN 201210542955 A CN201210542955 A CN 201210542955A CN 103859024 A CN103859024 A CN 103859024A
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- bifidobacterium
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- saccharomycete
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Abstract
The invention relates to a production method for fermented milk through symbiosis of bifidobacterium and yeast. The method utilizes the symbiotic relationship between bifidobacterium and yeast and allows bifidobacterium and yeast to ferment in milk together so as to produce the symbiotic fermented milk. The fermentated milk has pure flavor of lactic acid and aroma of yeast, can supplement beneficial substances like proteins and probiotics and complement a great amount of vitamin B1 and B2 produced in yeast fermentation at the same time and is functional food with good taste and rich nutrients.
Description
Technical field
The production method and the raw material proportioning that the invention provides a kind of Bifidobacterium, saccharomycete symbiotic fermentation breast, belong to food fermentation field.
Background technology
[bifidobacterium can produce lactic acid and acetic acid after fermenting in human body intestines, can improve the utilization rate of calcium, phosphorus, iron, promotes the absorption of iron and vitamin D.Bifidobacterium fermentation lactose produces galactolipin, is to form cerebronic composition in cerebral nervous system, has substantial connection with the ramp of baby due hindbrain.Bifidobacterium can produce Cobastab
1, B
2, B
6, B
12and the nutriment of the needed by human such as alanine, valine, aspartic acid and threonine, there is the important trophism can not be ignored for human body.
Bifidobacterium also has anti-aging effects micro-Ecological Investigation American-European and Japan and shows, the bifidobacteria in long lived elder ight soil with in, quite young.China scientist also obtained identical result to the investigation of Bama Area In Guangxi long lived elder.The antidotal reason of Bifidobacterium, is because Bifidobacterium can suppress spoilage organisms growth, reduces the generation of the harmful substances such as ammonia, hydrogen sulfide, indoles and scatol in its metabolite.
In addition Bifidobacterium causes that at control, the helicobacter pylori of phagopyrism the aspect such as control and ulcerative colitis control of gastritis (gastric ulcer etc.) all has suitable progress in recent years.Bifidobacterium is different from general medicine.General medicine is unidirectional treatment, such as hypotensive, reducing blood lipid, controls diarrhoea, treats constipation etc., and its effect is unidirectional.And the effect of Bifidobacterium is two-way adjustment, both can be by high having lowered, also can be by low going up, use bifidobacterium preparations may treat diarrhoea and constipation simultaneously, make human body recover normal, the effect that Here it is adjusts.Modern's rhythm of life is accelerated, and operating pressure increases, and the various gastrointestinal diseases that cause are therefrom especially general, and highly active elongated Bifidobacterium more can directly be supplemented beneficial bacterium, adjusts human body microecological balance.
Yeast is divided into two kinds, yeast cake, dry ferment, is a kind of edible, nutritious unicellular microorganism, in nutrition, it is called " inexhaustible nutrient source ".Except protein, carbohydrate, lipid, yeast is also rich in multivitamin, mineral matter and enzyme.Yeast after fermentation or a kind of very strong polyphenoils, can protect liver, has certain detoxication.The mineral energy such as selenium, chromium in yeast is anti-ageing, antitumor, prevention of arterial sclerosis, and improves the immunity of human body.After fermentation, in flour, a kind of phytic acid that affects the element absorption such as calcium, magnesium, iron can be decomposed, thereby improves absorption and the utilization of human body to these nutriments.
Bifidobacterium is anaerobic bacteria, need during the fermentation the environment of an anaerobism, saccharomycete can be survived in the environment of aerobic and anaerobic, therefore can be by both symbiotic fermentations, the oxygen of going out in raw material with saccharomycete, fermentation together with in the environment of Bifidobacterium in anaerobism again, thus multiple beneficial material produced.In prior art, the technique of bifidobacterium fermentation and saccharomycetes to make fermentation is very ripe, but does not also occur utilizing both symbiotic fermentations to produce the method for yoghurt product.
summary of the invention:
The production method and the raw material proportioning that the invention provides a kind of Bifidobacterium, saccharomycete symbiotic fermentation breast, raw material proportioning is: in raw milk, add the skimmed milk powder of 2-6%, the sucrose of 6-12%, the glucose of 1-3%, the vitamin C of 0.01-0.05%.It is anaerobic bacteria that the present invention utilizes Bifidobacterium, all survivable characteristics of saccharomycete aerobic anaerobic, by both symbiotic fermentations, earlier fermentation can utilize saccharomycete to raw material deoxygenation, and can produce lactose and create a favorable environment to Bifidobacterium, effectively improve the situation of the growthing lag of Bifidobacterium, later stage, both fermented until finish simultaneously, sour-sweet moderate with the yogurt taste of this explained hereafter, singly do not there is the pure local flavor of lactic acid, also there is the fragrance of yeast, at supplementary albumen, when the benefit materials such as probio, can supplement a large amount of Cobastabs that saccharomycetes to make fermentation produces
1, B
2, be the functional food that a kind of mouthfeel is good, nutritious.
Technical scheme of the present invention:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add the skimmed milk powder of 2-6%, the sucrose of 6-12%, the glucose of 1-3%, mix, homogeneous under the pressure of 15-20MPa, be heated to again 113-117 ℃, keep sterilization in 3-5 minute, then be cooled to 26-29 ℃, for subsequent use;
B, bifidobacterium bifidum is pressed to 6-8%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 2-4%, adds the vitamin C of the 0.01-0.05% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 26-29 ℃, continuing fermentation 100-140 minute;
D, the temperature of fermentation tank is increased to 35-39 ℃, continues fermentation 4-6 hour; Finish fermentation, be cooled to 8-11 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 1-5 ℃ of Storage in cold bank, be finished product.
The finished product obtaining by above technical scheme, the number of active bifid bacteria more than 1,000 ten thousand/ml, Cobastab
1content can reach 4-8ppm, Cobastab
2content be 35-50ppm.
The technical problem that the present invention solves:
1, the present invention utilizes Bifidobacterium and saccharomycete symbiotic fermentation effectively to improve the situation of the growthing lag of Bifidobacterium, produces sour power and improves, and has accelerated curdled milk, has shortened the production cycle.
2, two kinds of bacterium symbiotic fermentations, have not only increased the content of finished product protein, and have strengthened Cobastab
1and Cobastab
2.
3, two kinds of bacterium symbiotic fermentations, are finished product unique flavors, singly do not have the pure local flavor of lactic acid, also have the fragrance of yeast.
The specific embodiment
Embodiment 1:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add 2% skimmed milk powder, 6% sucrose, 1% glucose, mix homogeneous under the pressure of 15MPa, be heated to again 113 ℃, keep sterilization in 5 minutes, be then cooled to 26 ℃, for subsequent use;
B, by bifidobacterium bifidum by 6%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 2%, adds the vitamin C of 0.01% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 26 ℃, continuing fermentation 140 minutes;
D, the temperature of fermentation tank is increased to 35 ℃, continues fermentation 6 hours; Finish fermentation, be cooled to 8 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 1 ℃ of Storage in cold bank, be finished product.
The finished product that this embodiment obtains, the number of active bifid bacteria is 1,136 ten thousand/ml, Cobastab
1content can reach 4.9ppm, Cobastab
2content be 36.1ppm.
Embodiment 2:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add 6% skimmed milk powder, 12% sucrose, 3% glucose, mix homogeneous under the pressure of 20MPa, be heated to again 117 ℃, keep sterilization in 3 minutes, be then cooled to 29 ℃, for subsequent use;
B, by bifidobacterium bifidum by 8%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 4%, adds the vitamin C of 0.05% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 29 ℃, continuing fermentation 100 minutes;
D, the temperature of fermentation tank is increased to 39 ℃, continues fermentation 4 hours; Finish fermentation, be cooled to 11 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 5 ℃ of Storage in cold banks, be finished product.
The finished product that this embodiment obtains, the number of active bifid bacteria is 1,251 ten thousand/ml, Cobastab
1content can reach 6.6ppm, Cobastab
2content be 42.7ppm.
Embodiment 3:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add 4% skimmed milk powder, 10% sucrose, 2% glucose, mix homogeneous under the pressure of 18MPa, be heated to again 115 ℃, keep sterilization in 4 minutes, be then cooled to 28 ℃, for subsequent use;
B, by bifidobacterium bifidum by 7%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 3.5%, adds the vitamin C of 0.03% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 28 ℃, continuing fermentation 120 minutes;
D, the temperature of fermentation tank is increased to 37 ℃, continues fermentation 5 hours; Finish fermentation, be cooled to 9 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 3 ℃ of Storage in cold banks, be finished product.
The finished product that this embodiment obtains, the number of active bifid bacteria is 1,458 ten thousand/ml, Cobastab
1content can reach 7.9ppm, Cobastab
2content be 48.2ppm.
Embodiment 4:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add 3% skimmed milk powder, 9% sucrose, 2% glucose, mix homogeneous under the pressure of 17MPa, be heated to again 116 ℃, keep sterilization in 3 minutes, be then cooled to 27 ℃, for subsequent use;
B, by bifidobacterium bifidum by 6%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 3%, adds the vitamin C of 0.02% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 28 ℃, continuing fermentation 120 minutes;
D, the temperature of fermentation tank is increased to 38 ℃, continues fermentation 5 hours; Finish fermentation, be cooled to 10 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 2 ℃ of Storage in cold banks, be finished product.
The finished product that this embodiment obtains, the number of active bifid bacteria is 1,411 ten thousand/ml, Cobastab
1content can reach 7.3ppm, Cobastab
2content be 46.8ppm.
Embodiment 5:
A, by raw milk, skimmed milk power, sucrose, glucose by raw material proportioning: in raw milk, add 5% skimmed milk powder, 10% sucrose, 1% glucose, mix homogeneous under the pressure of 19MPa, be heated to again 114 ℃, keep sterilization in 4 minutes, be then cooled to 28 ℃, for subsequent use;
B, by bifidobacterium bifidum by 7%, saccharomyces lactis bacterium is inoculated in A step gained raw material by 3.5%, adds the vitamin C of 0.04% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 28 ℃, continuing fermentation 130 minutes;
D, the temperature of fermentation tank is increased to 37 ℃, continues fermentation 6 hours; Finish fermentation, be cooled to 10 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 2 ℃ of Storage in cold banks, be finished product.
The finished product that this embodiment obtains, the number of active bifid bacteria is 1,492 ten thousand/ml, Cobastab
1content can reach 7.8ppm, Cobastab
2content be 49.1ppm.
Claims (4)
1. a production method for Bifidobacterium, saccharomycete symbiotic fermentation breast, is characterized in that, raw materials used formula is: in raw milk, add the skimmed milk powder of 2-6%, the sucrose of 6-12%, the glucose of 1-3%, the vitamin C of 0.01-0.05%.
2. the production method of a kind of Bifidobacterium as claimed in claim 1, saccharomycete symbiotic fermentation breast, is characterized in that, described production method realizes by following processing step:
A, raw milk, skimmed milk power, sucrose, glucose are mixed by raw material proportioning claimed in claim 1, homogeneous under the pressure of 15-20MPa, then be heated to 113-117 ℃, and keeping 3-5 minute, sterilization, is then cooled to 26-29 ℃, for subsequent use;
B, Bifidobacterium is pressed to 6-8%, saccharomycete is inoculated in A step gained raw material by 2-4%, adds the vitamin C of the 0.01-0.05% after low temperature sterilization simultaneously, in fermentation tank, mixes;
C, the temperature of fermentation tank is remained on to 26-29 ℃, continuing fermentation 100-140 minute;
D, the temperature of fermentation tank is increased to 35-39 ℃, continues fermentation 4-6 hour; Finish fermentation, be cooled to 8-11 ℃;
E, by filling the cooled zymotic fluid of D step gained, put into 1-5 ℃ of Storage in cold bank, be finished product.
3. the production method of a kind of Bifidobacterium as claimed in claim 2, saccharomycete symbiotic fermentation breast, is characterized in that, the Bifidobacterium inoculating in B step is bifidobacterium bifidum, and the saccharomycete of inoculation is saccharomyces lactis bacterium.
4. the production method of a kind of Bifidobacterium as claimed in claim 2, saccharomycete symbiotic fermentation breast, is characterized in that, in finished product the number of active bifid bacteria more than 1,000 ten thousand/ml, Cobastab
1content be 4-8ppm, Cobastab
2content be 35-50ppm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108782758A (en) * | 2018-06-22 | 2018-11-13 | 陕西师范大学 | A kind of fermented type synbiotic goat milk powder and preparation method thereof |
| CN112280811A (en) * | 2020-10-31 | 2021-01-29 | 山东省大健康精准医疗产业技术研究院 | Method for high-yield production of short-chain fatty acid by utilizing microbial symbiotic fermentation technology |
-
2012
- 2012-12-15 CN CN201210542955.0A patent/CN103859024A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108782758A (en) * | 2018-06-22 | 2018-11-13 | 陕西师范大学 | A kind of fermented type synbiotic goat milk powder and preparation method thereof |
| CN108782758B (en) * | 2018-06-22 | 2022-03-08 | 浙江忠梦昌健康科技有限公司 | Fermented synbiotic goat milk powder and preparation method thereof |
| CN112280811A (en) * | 2020-10-31 | 2021-01-29 | 山东省大健康精准医疗产业技术研究院 | Method for high-yield production of short-chain fatty acid by utilizing microbial symbiotic fermentation technology |
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Application publication date: 20140618 |