CN103858916A

CN103858916A - Compound bactericide containing benomyl and diethofencarb

Info

Publication number: CN103858916A
Application number: CN201410112293.2A
Authority: CN
Inventors: 曹荣成
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-03-14
Filing date: 2014-03-14
Publication date: 2014-06-18

Abstract

The invention discloses a compound bactericide containing benomyl and diethofencarb. Besides containing benomyl and diethofencarb, the compound bactericide also contains a dispersing agent, nekal, a suspending agent and a filler. The compound bactericide is used for preventing and controlling gray mold on multiple plants, especially on flowers, can also can be used for various crops such as Chinese cabbage, cabbage, cauliflower, white gourd, cucumber, tomato, litchi fruit, grape, wheat, rapeseed plant, potato and tobacco; and the compound bactericide has good sterilizing effect and low cost and can effectively delay and prevent pesticide resistance development through field trial.

Description

A kind of composite bactericide that contains the mould prestige of benomyl second

Technical field

The invention belongs to pesticide production technology field, relate to a kind of bactericide, relate in particular to a kind of composite bactericide of preventing and treating crops gray mold.

Background technology

Benomyl, the special fourth imido grpup-3-isopropyl-5-of chemical name: 2-phenyl-3,4,5,6-tetrahydrochysene-2H-1,3,5-thiadiazine-4-ketone, structural formula is:

These product are efficient, wide spectrum, systemic fungicide, have protection, root out and therapeutic action, can be used for sprinkling, seed dressing and soil treatment.Be mainly used in preventing and treating vegetables, fruit tree, oil crop disease.

The mould prestige of second, chemical name: N-(3,4-diethoxy phenyl) carbamic acid isopropyl ester

Structural formula:

Effect feature is a kind of has the bactericide of negative cross resistance with carbendazim.Medicament enters the tubulin binding in somatic cells core, thereby affects the division of cell.This effect is very similar to the mode of action of thalline to carbendazim, but the two is not at same application point.As once botrytis cinerea develops immunity to drugs to carbendazim, and very sensitive to the mould prestige of second.On the contrary, to the botrytis cinerea of carbendazim sensitivity, the mould prestige of second shows as without bacteriostatic activity.

Controlling object and this agent of using method are not generally made single dose and are used, but prevent and treat gray mold with the pharmacy mix such as carbendazim, thiophanate, phonetic mould amine or procymidone.

Summary of the invention

The object of this invention is to provide a kind of bactericidal effect good, cost is low, can effectively delay and stop the composite bactericide of development of drug resistance.

The object of the invention is to be achieved through the following technical solutions, the invention provides a kind of composite bactericide, by the benomyl as active ingredient and composite the forming of the mould prestige of second.

Preferably, it contains benomyl 10-75%, the mould prestige 5-60% of second;

Preferably, it contains benomyl 20%, the mould prestige 25% of second.

The present invention also provides the wetting powder that utilizes above-mentioned composite bactericide to make simultaneously, and this wetting powder contains 20% benomyl and 25% the mould prestige of second, and diffusant, pulls open powder, suspending agent, filler;

Described diffusant is 1% sodium metnylene bis-naphthalene sulfonate;

The described powder that pulls open is 1% sodium butylnaphthalenesulfonate;

Described suspending agent is 4% lignin;

Described filler is 4% white carbon, 10% potter's clay;

Surplus is precipitated calcium carbonate.

Compound sterilizing wettable preparation of the present invention, can be used for the particularly control of the gray mold on flowers of various plants, also can be for other crops such as Chinese cabbage, wild cabbage, cauliflower, wax gourd, cucumber, tomato, lichee, grape, wheat, rape, potato, tobaccos.

The compound that composition of the present invention also can have desinsection or a bactericidal property with other particularly protective fungicide mixes use, also can use with nematocide, protective agent, growth regulator, nutrient for plants or soil conditioner mixture.Composition of the present invention can be used by common method, as cast, injection, spraying.Amount of application of the present invention is with weather condition or crop state variation, and time of application can be before or after crop be susceptible.The duration of protective effect is conventionally relevant with the content of individualized compound in composition, also relevant to extraneous factor, for example weather, but by using suitable formulation can slow down climatic influences.

The remarkable advantage of composition of the present invention: the one, composition shows extremely strong synergistic effect in certain ratio range, after mixing, the bactericidal effect of composition is significantly improved compared with its single dose, thereby reduce using dosage, in reducing peasant's drug cost, reduce influence degree.The 2nd, interracial structure differs larger, and the resistance that the application of said composition can delay single dose occurs and development.

Embodiment

Different pesticide active ingredients is combined and makes composite insecticide, is effectively and the efficiently mode of one that solves at present the series of problems such as germ pesticide resistance.

Inventor by a large amount of screening tests, finds that benomyl and the mould prestige composite bactericide of second have significant synergistic function to ash arrhizus bacteria etc., and is not only the simple addition of two kinds of medicaments, specifically sees embodiment.

Biologicall test example one: the composite Toxicity Determination to rose family ornamental flower red ash arrhizus bacteria of the four seasons of the mould prestige of benomyl and second.

Subjects: the ash arrhizus bacteria (Venturia nashicola) that picks up from field

Test method: spore germination method.Separate ash arrhizus bacteria from picking up from field leaf spot lesion, by germ on PDA medium in 25 ℃ of incubators continuous culture carry out purifying 3 times, and then be connected to shaken cultivation spore in PDB liquid nutrient medium.Cultured ash arrhizus bacteria spore is removed by filter to mycelia, be then mixed with the suspension of 4 × 105 spore/milliliters with sterile water.Reagent agent is diluted to corresponding concentration, and adds 2mL mixture with the spore suspension of ash arrhizus bacteria with 2mL, add 2mL spore suspension as blank take 2mL sterile water.Be placed in 24 ℃ of incubators and cultivate, microscopy spore germination rate after 15h, and calculate spore germination relative inhibition.10 visuals field of the random microscopy of every processing, repeat 4 times, and every spore germ tube length exceedes 1/2 of spore length and is germinating.

Relative inhibition (%)=(contrast spore germination rate-processing spore germination rate) ÷ contrasts spore germination rate × 100%

Spore germination relative inhibition is converted into probability value (y), liquor strength (μ g/ml) converts logarithm value (x) to, calculate concentration EC50 in virulence equation and inhibition with method of least squares, calculate toxicity index and the co-toxicity coefficient (CTC) of medicament according to the abundant method of Sun Yun.Toxicity test the results are shown in Table 1.

Actual measurement toxicity index (ATI)=(standard medicament EC50 ÷ reagent agent EC50) × 100

The percentage composition of B in percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI) the ÷ theoretical toxicity index of mixture (TTI)] × 100

CTC≤80, composition performance is antagonism; 80<CTC<120, composition performance is summation action; CTC >=120, composition performance is synergistic effect.

The composite Toxicity Determination result to red ash arrhizus bacteria of the four seasons of the mould prestige of benomyl and second

Process	EC50(μg/mL)	ATI	TTI	Co-toxicity coefficient CTC
					Benomyl	0.79	100.0	/	/
The mould prestige of second	4.33	18.2	/	/
					Benomyl 40%: the mould prestige 40% of second	0.65	121.8	98.5	123.7
Benomyl 50%: the mould prestige 30% of second	0.47	169.5	92.7	183.3
					Benomyl 10%: the mould prestige 45% of second	2.87	27.2	19.8	137.8
Benomyl 15%: the mould prestige 30% of second	0.46	162.4	79.6	204.2
					Benomyl 30%: the mould prestige 15% of second	1.56	50.1	25.6	196.1
Benomyl 20%: the mould prestige 25% of second	0.58	132.3	59.1	224.2

Benomyl 35%: the mould prestige 45% of second	1.96	39.6	22.2	180.4
					Benomyl 75%: the mould prestige 5% of second	3.36	23.4	19.3	121.3

As can be seen from Table 1, the mould prestige of benomyl and second is respectively with benomyl 40%: the mould prestige 40% of second, benomyl 50%: the mould prestige 30% of second, benomyl 10%: the mould prestige 45% of second, benomyl 15%: the mould prestige 30% of second, benomyl 30%: the mould prestige 15% of second, benomyl 20%: the mould prestige 25% of second, benomyl 35%: the mould prestige 45% of second, benomyl 75%: the co-toxicity coefficient of the mould prestige of second 5% ratio when composite is respectively 123.7,183.3,137.8,204.2,196.1,224.2,180.4,121.3, its co-toxicity coefficient is all greater than 120, shows as synergistic effect.Co-toxicity coefficient when wherein the mould prestige of benomyl and second is composite with 20:25 ratio is the highest, and synergistic effect is remarkable.

Composite bactericide of the present invention can be prepared into any one formulation that is applicable to agricultural use by known method, reasonable formulation is wetting powder, water dispersible granules, suspending agent, oil-suspending agent, wherein, through overtesting, reasonable formulation is wetting powder.

Embodiment 1: the mould prestige wetting powder of benomyl second

Benomyl 20%

The mould prestige 25% of second

Sodium metnylene bis-naphthalene sulfonate 1%

Sodium butylnaphthalenesulfonate 1%

Lignin 4%

White carbon 3%

Potter's clay 10%

Precipitated calcium carbonate complements to 100%

Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of formula, after superfine jet mill is pulverized, obtain the mould prestige wetting powder of benomyl second.

This embodiment is applied to control red gray mold of the four seasons.The mould prestige wetting powder of benomyl second is pressed the spraying of 40ga.i./ha thin up, and after medicine, the control efficiency of 7 days is 92.6%.The composite rear synergistic effect of the mould prestige of benomyl second is obvious, and to the control efficiency of gray mold, significantly better than single dose, and the consumption of active ingredient obviously reduces, thereby reduced cost and alleviated the pollution to environment.

Claims

1. contain a composite bactericide for the mould prestige of benomyl second, it is characterized in that by the benomyl as active ingredient and composite the forming of the mould prestige of second.

2. a kind of composite bactericide that contains the mould prestige of benomyl second as claimed in claim 1, is characterized in that it contains benomyl 10-75%, the mould prestige 5-60% of second.

3. a kind of composite bactericide that contains the mould prestige of benomyl second as claimed in claim 1, is characterized in that it contains benomyl 20%, the mould prestige 25% of second.