CN103857805B - 聚合酶组合物、制备和使用所述聚合酶组合物的方法 - Google Patents

聚合酶组合物、制备和使用所述聚合酶组合物的方法 Download PDF

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Publication number
CN103857805B
CN103857805B CN201280048344.9A CN201280048344A CN103857805B CN 103857805 B CN103857805 B CN 103857805B CN 201280048344 A CN201280048344 A CN 201280048344A CN 103857805 B CN103857805 B CN 103857805B
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polymerase
seq
amino acid
modified
polymerases
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CN103857805A (zh
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P·范德霍恩
T·尼基弗罗夫
罗国斌
M·兰狄斯
D·马祖尔
E·托泽
T·林斯库姆
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Life Technologies Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polyesters Or Polycarbonates (AREA)
CN201280048344.9A 2011-08-10 2012-08-10 聚合酶组合物、制备和使用所述聚合酶组合物的方法 Active CN103857805B (zh)

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US201161522125P 2011-08-10 2011-08-10
US61/522,125 2011-08-10
US201161545434P 2011-10-10 2011-10-10
US61/545,434 2011-10-10
US201261585133P 2012-01-10 2012-01-10
US61/585,133 2012-01-10
US201261643844P 2012-05-07 2012-05-07
US61/643,844 2012-05-07
US201261681593P 2012-08-09 2012-08-09
US61/681,593 2012-08-09
PCT/US2012/050423 WO2013023176A2 (en) 2011-08-10 2012-08-10 Polymerase compositions, methods of making and using same

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US (4) US20130040365A1 (https=)
EP (2) EP2742151B1 (https=)
CN (2) CN103857805B (https=)
IN (1) IN2014CN01834A (https=)
LT (1) LT2742151T (https=)
WO (1) WO2013023176A2 (https=)

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CN107326079A (zh) 2017-11-07
US10633641B2 (en) 2020-04-28
WO2013023176A2 (en) 2013-02-14
US12338465B2 (en) 2025-06-24
US9976128B2 (en) 2018-05-22
US20130040365A1 (en) 2013-02-14
LT2742151T (lt) 2018-02-12
EP3293274A3 (en) 2018-03-28
US20180305673A1 (en) 2018-10-25
IN2014CN01834A (https=) 2015-05-29
EP2742151A2 (en) 2014-06-18
CN103857805A (zh) 2014-06-11
EP2742151B1 (en) 2017-10-25
WO2013023176A3 (en) 2013-04-18
EP3293274B1 (en) 2020-04-01
EP3293274A2 (en) 2018-03-14
CN107326079B (zh) 2021-04-20
US20220162573A1 (en) 2022-05-26
US20150368626A1 (en) 2015-12-24

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