CN103849570A - Method for harvesting microalgae by rapidly adsorbing and gathering with suspended carrier - Google Patents
Method for harvesting microalgae by rapidly adsorbing and gathering with suspended carrier Download PDFInfo
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- CN103849570A CN103849570A CN201410075064.8A CN201410075064A CN103849570A CN 103849570 A CN103849570 A CN 103849570A CN 201410075064 A CN201410075064 A CN 201410075064A CN 103849570 A CN103849570 A CN 103849570A
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Abstract
The invention discloses a method for harvesting microalgae by rapidly adsorbing and gathering with a suspended carrier. The method comprises the following steps: when microalgae is cultured to a target harvesting period, adding the carrier into a microalgae culture solution to suspend the carrier in the microalgae culture solution; rapidly adsorbing and gathering the microalgae by using relative movement of the carrier and the microalgae; separating the carrier and the microalgae to harvest the microalgae. By adopting the method, continuous, convenient and economical large-scale culture and harvest of microalgae can be realized.
Description
Technical field
The production results technical field that the present invention relates to micro-algae, is specifically related to a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption.
Background technology
Some microalgae cell includes grease at high proportion, can production biofuel by fat transformation, replace traditional Nonrenewable energy resources such as coal and oil as renewable energy source, and alleviate the energy dilemma of global scale.In addition, some microalgae cells, for example Haematocoocus Pluvialls, at energy synthesizing astaxanthin stationary phase, has pharmaceutical use.The heavy industrialization cultivation that realizes micro-algae has certain economic worth and the ecological value.
Microalgae cell volume is little, and diameter is about 3-10 μ m.In micro-algae is cultivated, micro-algae enrichment concentration process is one of key issue of its heavy industrialization application of restriction.Existing microalgae harvesting method has filtration, air supporting, centrifugal and sedimentation etc., but current method all exists certain problem.Filter and easily cause filter net jam, and collection effciency is lower.Increase pressure can raise microalgae harvesting cost and energy consumption, increase the requirement of the mechanical U intensity to filter screen simultaneously.Centrifugal separation can effectively separate micro-algae, but the required energy consumption of the method is very large, and cost is high, thereby affects the extensive commercial application of the method.High speed centrifugation method is likely destroyed cell walls and is even caused cytoclasis.By Bubble-floating Method is the more potential micro-algae concentration method of one, but this method need have rational air-flotation separation equipment and separating technology, and separating effect is subject to the many factors such as device structure and processing condition.Sedimentation is by regulating pH or add inorganic or organic compound to nutrient solution, making micro-algae generation flocculating settling.The introducing of coagulating agent can affect micro-algae quality, causes micro-algae subsequent applications processing influenced.In addition, also have some novel methods as, flocculation of electric field and utilize fungi to mediate micro-algae balling-up etc., all in conceptual phase.The present invention is intended to realize by adding suspending carrier to culture system the problem of quick adsorption trapping harvesting microalgae.
Summary of the invention
The problem existing in order to solve above-mentioned prior art, the object of the present invention is to provide a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption, can realize continuous, convenient, micro-algae large scale culturing and results economically.
For reaching above object, the present invention adopts following technical scheme:
A kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption, in the time that micro-algae is cultured to target results periods, by add carrier in micro algae culturing liquid, carrier is suspended in micro algae culturing liquid, utilizing the relative movement of carrier and micro-algae to realize carrier catches the quick adsorption of micro-algae, and then by the separating of carrier and micro-algae, realize the results of micro-algae.
The specific surface area of described carrier is large, quality is harder, is shaped as thread, spherical or polyhedron; Material is fiber crops, cotton, mohair; The dosage of carrier accounts for the long-pending 20-40% of micro-frond.
Removing carrier after microalgae cell can recycling, again puts in the middle of micro algae culturing liquid, and liquid phase frustule continues as seed, is again trapped by carrier adsorption, realizes cultured continuously and the results of microalgae cell.
The described mode that carrier is suspended in micro algae culturing liquid is aeration power, shaking table power or ties up fixing.
The aeration gas of described aeration power is air or carbon dioxide, in aeration process, increase the mobility of microalgae cell, increase the probability that microalgae cell is caught and adhered to by carrier, microalgae cell on carrier can utilize carbonic acid gas supplementary in aeration process to realize the regrowth after adhering to, algae film thickness on carrier is increased, be convenient to results.
The generation type of described carrier and micro-algae relative movement is aeration power, shaking table power or flow.
The harvesting approach of described micro-algae is: micro-algae forms after certain thickness algae film on carrier, by fishing for or holding back and realize results to microalgae cell carrier.
The described above microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption, can be used in that heterotrophism is cultivated and photosynthesis cultivate be autotrophy cultivate, for the cultivation of the micro-algae of the energy and secrete the production of chemical substance for micro-algae.
Compared to the prior art, tool has the following advantages in the present invention:
1), compared with existing membrane filtration harvesting microalgae method, the inventive method is without problems such as filter net jam in membrane filtration processes, collection effciency are lower, without increasing system pressure.Therefore, can reduce microalgae harvesting cost and energy consumption.
2) compared with existing centrifugation harvesting microalgae method, the inventive method has broken that the required energy consumption of centrifugal separation method is very large, and cost is high, thereby affects the restriction of the extensive commercial application of the method.Do not exist high speed centrifugation condition to destroy cell walls and even cause cytoclastic possibility, can gather in the crops intact cell simultaneously.
3) compared with existing air supporting harvesting microalgae method, the inventive method equipment and separating technology are simple, easy to operate, the stable easily control of efficiency of crop.
4) compared with existing coagulating sedimentation harvesting microalgae method, the inventive method, without the introducing of coagulating agent, can not affect micro-algae quality, can not cause micro-algae subsequent applications processing influenced.
Brief description of the drawings
Fig. 1 is the course of processing schematic diagram of adsorbing and trapping material of the present invention.
Fig. 2 is that schematic diagram is cultivated in the embodiment of the present invention 1 concussion.
Fig. 3 is the embodiment of the present invention 1 solid liquid phase algae total amount graphic representation over time.
Fig. 4 is water sample variation diagram before and after the embodiment of the present invention 2 adsorbing and trappings, wherein: Fig. 4 a is water sample before adsorbing and trapping, and Fig. 4 b is water sample after adsorbing and trapping.
Embodiment
Below in conjunction with drawings and the specific embodiments, the present invention is described in further detail.
The preparation of carrier: by the Performance Ratio of ten different materials, select the scleroid materials of bigger serface such as mohair, cotton.Be cut into some sections of the long segments of 5cm.As shown in Figure 1, tied up balling-up, bunchy or in blocks.In cultivating, laboratory Erlenmeyer flask directly adds spheroidal material; The formula application of the extensive runway pool, is placed on some carrier stacks in blocks in the runway pool, and micro-algae is adsorbed and caught.
Adding of carrier: target microalgae is cultured under certain condition and can be gathered in the crops the stage.As intermittently cultivated, extract grease in frustule, gather in the crops in mid-term stationary phase.Harvest time is depending on cultivating object.In the time that micro-algae is cultured to target results periods, add carrier, the dosage of carrier accounts for the long-pending 20-40% of micro-frond.
The setting of adsorbing and trapping condition: realize the concussion among a small circle of carrier by exterior power.Laboratory scale results process can adopt shaking table concussion.In earthquake process, between carrier and microactuator suspension frustule, produce relative movement, carrier is similar to the high density macromole polymer in precipitation process of coagulation, in moving process, suspension frustule is played to net and catches the effect of sweeping of returning; Meanwhile, between frustule and carrier, also there is initiatively adsorbing existence, and then promote the results process of micro-algae.The results of utilizing carrier to realize the micro-algae in the runway pool are to produce the relative movement between micro-algae and suspending carrier by the effect of current, realize the absorption of micro-algae is caught.Can be by regulating the parameter regulation microalgae harvesting speed such as reflux ratio, flow rate of water flow.
Carrier separates from algae culture system to be realized the separation of micro-algae and concentrates.Micro-algae that remaining suspension is not gathered in the crops can be used as seed and proceeds enlarged culturing.By with upper type, Reasonable Regulation And Control results frequency and results ratio, can realize continuous, convenient, micro-algae large scale culturing economically.
Embodiment 1: intermittently microalgae harvesting application on a small scale
In each Erlenmeyer flask, pour the mBG11 nutrient solution 200ml preparing into, and inoculate grid algae Scenedesmus.LX1, starting point concentration is about 1 × 10
4individual/ml, is placed in photo bio incubator and cultivates.Cultivate after 10 days liquid phase algae density reachable 3.4 × 10
6individual/mL.In algae liquid, add spherical adsorbing and trapping carrier (each suspending carrier is tied up and formed by 100 × 5cm filamentary material) to be placed in concussion incubator, as shown in Figure 2.Adjusting vibration frequency is 100rad/min, and sampling, observes and record solid liquid phase algae quantity over time, as shown in Figure 3 at regular intervals.Visible, cultivate after 24h when concussion, can realize more than 95% yield rate.
Embodiment 2: medium-sized microalgae harvesting application
Utilize bioreactor to cultivate micro-algae.The 10L mBG11 substratum preparing is injected in the middle of bioreactor, and inoculate appropriate logarithmic phase grid algae S.LX1, make its starting point concentration be about 1 × 10
4individual/ml.Culture condition is Light To Dark Ratio 14:10, light intensity 800～1300Lux, and temperature is room temperature.Micro-algae is cultured to mid-term stationary phase, and (algae density is about 4 × 10
6individual/ml), in reactor, add 7 of pencil carrier balls (each suspending carrier is tied up and formed by 100 × 5cm filamentary material), utilize metal binding belt to be fixed in the middle of bioreactor.Make suspending carrier and frustule have certain relative movement by the mode of bottom aeration.After 4h, get liquid sample and carry out micro-algae counting, algae density approximately 1.5 × 10
5individual/mL.Visible, solid phase adsorption capture rate can reach more than 96%.Water sample before and after adsorbing and trapping as shown in Figure 4.
Claims (8)
1. one kind is utilized the microalgae harvesting method of suspending carrier quick adsorption trapping, it is characterized in that: in the time that micro-algae is cultured to target results periods, by add carrier in micro algae culturing liquid, carrier is suspended in micro algae culturing liquid, utilizing the relative movement of carrier and micro-algae to realize carrier catches the quick adsorption of micro-algae, and then by the separating of carrier and micro-algae, realize the results of micro-algae.
2. a kind of microalgae harvesting method that utilizes suspending carrier quick adsorption trapping according to claim 1, is characterized in that: the specific surface area of described carrier is large, quality is harder, is shaped as thread, spherical or polyhedron; Material is fiber crops, cotton, mohair; The dosage of carrier accounts for the long-pending 20-40% of micro-frond.
3. a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption according to claim 1, it is characterized in that: removing carrier after microalgae cell can recycling, again put in the middle of micro algae culturing liquid, liquid phase frustule continues as seed, again trapped by carrier adsorption, realize cultured continuously and the results of microalgae cell.
4. a kind of microalgae harvesting method that utilizes suspending carrier quick adsorption trapping according to claim 1, is characterized in that: described in carrier is suspended in micro algae culturing liquid mode be aeration power, shaking table power or tie up fixing.
5. require a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption described in 4 according to right U, it is characterized in that: the aeration gas of described aeration power is air or carbon dioxide, in aeration process, increase the mobility of microalgae cell, increase the probability that microalgae cell is caught and adhered to by carrier, microalgae cell on carrier can utilize carbonic acid gas supplementary in aeration process to realize the regrowth after adhering to, and the algae film thickness on carrier is increased, and is convenient to results.
6. a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption according to claim 1, is characterized in that: the generation type of described carrier and micro-algae relative movement is aeration power, shaking table power or flow.
7. a kind of microalgae harvesting method that utilizes the trapping of suspending carrier quick adsorption according to claim 1, it is characterized in that: the harvesting approach of described micro-algae is: micro-algae forms after certain thickness algae film on carrier, by fishing for or holding back and realize results to microalgae cell carrier.
8. the microalgae harvesting method that utilizes suspending carrier quick adsorption trapping described in claim 1 to 7 any one, is characterized in that: can be used in that heterotrophism is cultivated and photosynthesis cultivate be autotrophy cultivate, for the cultivation of the micro-algae of the energy and secrete the production of chemical substance for micro-algae.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995248A (en) * | 2017-04-26 | 2017-08-01 | 水利部交通运输部国家能源局南京水利科学研究院 | A kind of green safety, quick microalgae harvesting method |
| CN110218655A (en) * | 2019-07-11 | 2019-09-10 | 哈尔滨工业大学（深圳） | A kind of system using magnetic rotor harvest municipal sewage adhesion type culture oil-containing micro-algae |
| CN113173676A (en) * | 2021-04-09 | 2021-07-27 | 山东大学 | Low-consumption green system and method for recycling carbon, nitrogen and phosphorus resources in sewage |
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| WO2012171123A1 (en) * | 2011-06-13 | 2012-12-20 | Al-G Technologies Inc. | Method using immobilized algae for production and harvest of algal biomass and products |
| WO2013055887A1 (en) * | 2011-10-14 | 2013-04-18 | Regents Of The University Of Minnesota | Microalgae culture and harvest |
| CN103409321A (en) * | 2013-07-26 | 2013-11-27 | 清华大学 | Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier |
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Patent Citations (3)
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| WO2012171123A1 (en) * | 2011-06-13 | 2012-12-20 | Al-G Technologies Inc. | Method using immobilized algae for production and harvest of algal biomass and products |
| WO2013055887A1 (en) * | 2011-10-14 | 2013-04-18 | Regents Of The University Of Minnesota | Microalgae culture and harvest |
| CN103409321A (en) * | 2013-07-26 | 2013-11-27 | 清华大学 | Microalgae suspension-adhesion mixed culture and separated harvesting method based on suspended carrier |
Non-Patent Citations (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995248A (en) * | 2017-04-26 | 2017-08-01 | 水利部交通运输部国家能源局南京水利科学研究院 | A kind of green safety, quick microalgae harvesting method |
| CN110218655A (en) * | 2019-07-11 | 2019-09-10 | 哈尔滨工业大学（深圳） | A kind of system using magnetic rotor harvest municipal sewage adhesion type culture oil-containing micro-algae |
| CN110218655B (en) * | 2019-07-11 | 2020-11-06 | 哈尔滨工业大学（深圳） | System for attached culture of oil-containing microalgae by utilizing magnetic rotor to harvest municipal sewage |
| CN113173676A (en) * | 2021-04-09 | 2021-07-27 | 山东大学 | Low-consumption green system and method for recycling carbon, nitrogen and phosphorus resources in sewage |
| CN113173676B (en) * | 2021-04-09 | 2023-03-03 | 山东大学 | Low-consumption green system and method for recycling carbon, nitrogen and phosphorus resources in sewage |
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Application publication date: 20140611 |