CN103848111B - A kind of serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live the method for seed preservation - Google Patents
A kind of serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live the method for seed preservation Download PDFInfo
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- CN103848111B CN103848111B CN201210496811.6A CN201210496811A CN103848111B CN 103848111 B CN103848111 B CN 103848111B CN 201210496811 A CN201210496811 A CN 201210496811A CN 103848111 B CN103848111 B CN 103848111B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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Abstract
The invention belongs to field of marine biotechnology, the method that specifically one produces glue orchella serration Eucheuma muricatum (Gmel.) Web.Van Bos. (Eucheuma denticulatum) On Transportaion of Live and seed preservation.Serration Eucheuma muricatum (Gmel.) Web.Van Bos. is placed in the storage device of 17.0 23.0 DEG C after water processes dark condition and with moisturizing material one under the relative humidity of 80 ± 20%, and then reaches to extend storage time and long-time On Transportaion of Live serration Eucheuma muricatum (Gmel.) Web.Van Bos. purpose.The inventive method is simple, the keep-alive phase is long, it is adaptable to solve the long-distance transport of serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling, and at typhoon period, it is ensured that raiser gathers in the crops seedling in advance, and reaches safe storage seedling, evades the purpose that seedling is lost.
Description
Technical field
The invention belongs to field of marine biotechnology, specifically one produces glue orchella serration Eucheuma muricatum (Gmel.) Web.Van Bos.
(Eucheuma denticulatum) On Transportaion of Live and the method for seed preservation.
Background technology
Serration Eucheuma muricatum (Gmel.) Web.Van Bos. is the primary raw material producing ι-carrageenan in the world.And ι-carrageenan is very suitable for processing half admittedly
Body and liquid fruit juice, beverage, flavoring agent, toothpaste, cosmetics etc., in medical industry, vegetalitas film coating has nothing
Cross-linking reaction, medicine moisture absorption infectious disease risk low, non-animal derived, be difficult to containing aldehyde radical drug reaction and be prone to obsolete material
Recycling and profit advantages of higher, and ι type carrageenan is the best vegetable raw material preparing medical material coating.Serration kylin in the world
Dish cultivation is many to be carried out in subtropical and tropical zones, and serration Eucheuma muricatum (Gmel.) Web.Van Bos. main product is in the country in Southeast Asia such as Indonesia and Philippine
And area.In China, serration Eucheuma muricatum (Gmel.) Web.Van Bos., in addition to producing ι carrageenan and being used for food and medicine processing and manufacturing, also can be used directly to add
Work becomes cold and dressed with sauce instant product, one China of the most cold and dressed with sauce instant product to consume about 2000 tons of dry products every year.Before 2008, China is also
Not having serration Eucheuma muricatum (Gmel.) Web.Van Bos. artificial culture, raw material fully relies on import.In June, 2008, the Institute of Oceanology of the Chinese Academy of Sciences was by this algae
Introduce, be successfully made large-scale planting in Li An bay, Lingshui, Hainan and promote.
The cultivation of serration Eucheuma muricatum (Gmel.) Web.Van Bos. is vulnerable to the harm such as fish evil and typhoon, often occurs massive mortality to cause seedling tight
Lack.For this reason, it may be necessary to every now and then from other cultivation area live transport seedling, to maintain cultivation scale.Due to high density serration Qi
Unicorn dish easily secrets out of halogenated hydrocarbons in static sea water, quickly causes self frond deliquescing to be rotted, and therefore transport is (the most long-distance
Transport) during, how keep-alive serration Eucheuma muricatum (Gmel.) Web.Van Bos. is one of this vital technology of algae cultivation, does not the most also have
The method seeing the relevant effective On Transportaion of Live of serration Eucheuma muricatum (Gmel.) Web.Van Bos..In the past during this Ottelia acuminata (Gagnep.) Dandy seedling is transported for long-distance, often due to warm
Spending height and cause death of rotting, or cause Ottelia acuminata (Gagnep.) Dandy to damage to plants caused by sudden drop in temperature even freeze injury because cooling is improper, result also causes Ottelia acuminata (Gagnep.) Dandy vigor
Decline；Equally, during water transport is defeated, humid control is extremely important, and concrete technology yet there are no specifying information.
The temperature of serration Eucheuma muricatum (Gmel.) Web.Van Bos. suitable growth is generally 20.0-30.0 DEG C, only from ocean temperature angle analysis, although I
State is perennial meets the marine site of this seaweed growth seldom, and only Sanya, Hainan and Lingshui can meet the perennial life of serration Eucheuma muricatum (Gmel.) Web.Van Bos.
Long.But from spring Mo to autumn, there is in China the water temperature in vast sea area to maintain 20.0-30.0 DEG C, serration Eucheuma muricatum (Gmel.) Web.Van Bos. can be met
Scale is cultivated.For solving China this algae there is lack of raw materials situation, sea, China north and south temperature seasonal variation can be made full use of, when north in summer
When side's marine site temperature conditions is suitable, serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling is transported to the north from south and carries out scale cultivation, be final solution
The important channel of this algae of China there is lack of raw materials situation.It is necessary for this to develop a kind of technology ensureing this Sargassum On Transportaion of Live.
On the other hand, the Perenniporia martius countries and regions of cultivation serration Eucheuma muricatum (Gmel.) Web.Van Bos., are also the multiple districts of typhoon.How to exist
Before typhoon, results safe storage high-volume serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling, be also to evade typhoon lose and maintain typhoon in time
Later the important measures of fast quick-recovery serration Eucheuma muricatum (Gmel.) Web.Van Bos. cultivation scale.
Summary of the invention
A kind of method that it is an object of the invention to provide serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live and seed preservation.
The technical solution used in the present invention is for achieving the above object:
A kind of serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live the method for seed preservation, by serration Eucheuma muricatum (Gmel.) Web.Van Bos. from water process after with moisturizing material
Material one is placed in the storage device of 17.0-23.0 DEG C dark condition and under the relative humidity of 80 ± 20%, and then reaches to extend
Storage time and long-time On Transportaion of Live serration Eucheuma muricatum (Gmel.) Web.Van Bos. purpose.
By healthy serration Eucheuma muricatum (Gmel.) Web.Van Bos. Sargassum seedling after sea gathers in the crops, drain rapidly the moisture on surface, the degree drained
It is as the criterion not drip, stand-by.
Described storage device is put into the water suction moisturizing material soaking sea water in advance.
Described water suction moisturizing material can be: Caulis et Folium Lini, Boehmeria, cotton, and the various preferable cloth of chemical fibre water absorption；Soak
Water suction moisturizing material humidity after sea water, is as the criterion to drip the most outward.
The serration Eucheuma muricatum (Gmel.) Web.Van Bos. processed from water is put in storage device, is containing storage container bottom, surrounding and serration Eucheuma muricatum (Gmel.) Web.Van Bos.
The moisturizing absorbent material that sea water immersion is crossed is placed on middle or top.
Advantage for present invention:
On Transportaion of Live the most of the present invention and the method storing serration Eucheuma muricatum (Gmel.) Web.Van Bos., for the long-distance transport of serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling,
And gather in the crops in time and safe storage serration Eucheuma muricatum (Gmel.) Web.Van Bos. plants vegetables at typhoon period, as the seedling of Ottelia acuminata (Gagnep.) Dandy reproduction cultivation.
2. the present invention is directed to the highdensity serration Eucheuma muricatum (Gmel.) Web.Van Bos. feature that easy deliquescing is rotted in static sea water, through not
After temperature, humidity and the photo-irradiation treatment condition experiment of gradient, it is determined that one can On Transportaion of Live and storage serration Eucheuma muricatum (Gmel.) Web.Van Bos.
Technical method.
3. the present invention takes the technical measures from water, moisturizing, temperature control and dark.From water purpose for preventing serration Eucheuma muricatum (Gmel.) Web.Van Bos. from existing
In water, discharge the frond that causes of halogenated hydrocarbons when sea water middle-high density stores rot problem, extend the guarantor stored and in transportation
Live time；Moisturizing can prevent serration Eucheuma muricatum (Gmel.) Web.Van Bos. to be dried, and maintains its analytic metabolism ability together with temperature control；And dark can avoid light loss
Wound occurs, and reaches equally to extend the purpose storing and transporting the keep-alive time.
4. the inventive method is simple, the keep-alive phase is long, it is adaptable to solve the long-distance transport of serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling, and
At typhoon period, it is ensured that raiser gathers in the crops seedling in advance, and reaches safe storage seedling, evade the purpose that seedling is lost.
Detailed description of the invention
Keep-alive of the present invention stores the technical method of serration Eucheuma muricatum (Gmel.) Web.Van Bos.:
(1) by healthy serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling, from sea, results are placed on some string bags, drain the moisture on surface extremely
No longer drip with water and be as the criterion；Serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling after draining is loaded in Sheng storage container respectively, stores experiment as next step
Material.In operation, the Ottelia acuminata (Gagnep.) Dandy seedling after should as far as possible avoiding results is placed under intense direct illumination, unnecessary to reduce
Photic damage.
(2), under the conditions of Sheng storage container being put in different temperatures, humidity and lighting gradients, after storage different time, take out
And it being placed on rapidly marine cultivation, the growing state cultivating two weeks internal serration Eucheuma muricatum (Gmel.) Web.Van Bos. to plunge into the commercial sea evaluates treatment effect quality.
The thermograde setting carrying out experiment is respectively as follows: 14.0-32.0 DEG C；Relative humidity in the range of 60%-100%, light
It is set to dark or indoor dark light according to gradient.
(3) high-volume stores and during transport, is planted vegetables by serration Eucheuma muricatum (Gmel.) Web.Van Bos. and processes and put into Sheng storage appearance according to step (1) from water
In device, simultaneously at the moisturizing absorbent material containing in the middle of storage container bottom, surrounding and Ottelia acuminata (Gagnep.) Dandy or top placement sea water immersion is crossed, these
Material includes but not limited to Caulis et Folium Lini, Boehmeria, cotton and the various chemical fibre preferable clothes of water absorption etc., to ensure Ottelia acuminata (Gagnep.) Dandy in container
It is in certain humidity；The immersion degree of moisturizing material is the most outwards to seep water as basic standard.
(4) in whole operating process, should also be noted that and the most firmly extrude Sargassum, it is to avoid Sargassum fractures and other damage is sent out
Raw.
Pass through serial experiment, it is determined that it is temperature 17.0-23.0 DEG C that keep-alive stores the optimum condition of serration Eucheuma muricatum (Gmel.) Web.Van Bos., relatively
Humidity 80 ± 20%, dark or more weak indoor lighting conditions.The Ottelia acuminata (Gagnep.) Dandy transported under the conditions of above-mentioned and store, at least in 5 days
Remain to ensure its vitality, marine cultivation can be continued on for as seedling.
Embodiment 1
(1) take serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling 30 strain (every strain 30g) of health, drain the moisture on surface, will wherein 5 strains directly put
It is placed in and marine keeps hanging cultivation, as experiment contrast.It addition, other 25 strain serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling is loaded on (foam in bubble chamber
The widest 50cm of case size × long 80cm × high 30cm) store from water.
Before seedling puts into bubble chamber, bottom bubble chamber, place towel wet from sea water as water-keeping material, place one layer
Overlay one layer of wet towel after seedling then at seedling top, be then further continued for putting in case by remaining seedling, finally on seedling
Portion repaves one layer of wet towel, and the humidity of towel is as the criterion to drip the most outward.
During aforesaid operations, it is to avoid firmly extruding Sargassum causes the unnecessary equivalent damage that fractures to occur.
(2) bubble chamber is placed on temperature 14.0 ± 1.5 DEG C and in illumination is dark incubator, stores different time
(my god), random taking-up serration Eucheuma muricatum (Gmel.) Web.Van Bos., take out 5 strains every time and keep hanging in marine cultivation, measure it and keep hanging process the last fortnight marine
Weight change, observe its vitality, result ginseng table 1.
(3) in experimentation, through repeatedly measuring the relative humidity in bubble chamber, result display relative humidity is at 60%-100%
In the range of change, this variation and bubble chamber degree of closure and put into soak absorbent material quantity relevant, generally closing is preferable
In case, relative humidity is at 80%-100%, and during case-opening goods-taking dish, the relative humidity around Ottelia acuminata (Gagnep.) Dandy drops to about 60%.
Under table 1.14.0 ± 1.5 DEG C and dark condition, store the serration Eucheuma muricatum (Gmel.) Web.Van Bos. of different time from water, at sea keep hanging cultivation
Fresh weight change in training two weeks
Embodiment 2
(1) sample according to method in embodiment 1, place serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling and lay towel wet from sea water.
(2) bubble chamber is placed on temperature 17.0 ± 1.5 DEG C and in illumination is dark incubator, is storing different time
(my god), take 5 strain serration Eucheuma muricatum (Gmel.) Web.Van Bos. at random, again keep hanging in marine cultivation, measure serration Eucheuma muricatum (Gmel.) Web.Van Bos. at the marine the last fortnight that keeps hanging
Weight, observes its vigor, and result is as shown in table 2.
Table 2., at temperature 17.0 ± 1.5 DEG C and dark condition, stores the serration Eucheuma muricatum (Gmel.) Web.Van Bos. of different time, at sea from water
Keep hanging cultivation interior fresh weight change in two weeks
Embodiment 3
(1) sample according to method in embodiment 1, place serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling and lay towel wet from sea water.
(2) bubble chamber is placed on temperature 23.0 ± 1.5 DEG C and in illumination is dark incubator, stores different time
(my god), random take out serration Eucheuma muricatum (Gmel.) Web.Van Bos., take 5 strains every time and again keep hanging in marine, measure serration Eucheuma muricatum (Gmel.) Web.Van Bos. before marine keeping hanging two
The weight in week, analyzes the vitality situation of Ottelia acuminata (Gagnep.) Dandy, and result is as shown in table 3.
Table 3., at temperature 23.0 ± 1.5 DEG C and dark condition, stores the serration Eucheuma muricatum (Gmel.) Web.Van Bos. of different time, at sea from water
Keep hanging cultivation interior fresh weight change in two weeks
Embodiment 4
(1) sample according to method in embodiment 1, place serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling and lay towel wet from sea water.
(2) bubble chamber is placed on temperature 26.0 ± 1.5 DEG C and in illumination is dark incubator, stores different time
(my god), take 5 strain serration Eucheuma muricatum (Gmel.) Web.Van Bos. the most at random and again keep hanging in marine, measure serration Eucheuma muricatum (Gmel.) Web.Van Bos. at the marine weight keeping hanging the last fortnight
Amount, observes the upgrowth situation of Ottelia acuminata (Gagnep.) Dandy, and result is as shown in table 4.
Table 4., at temperature 26.0 ± 1.5 DEG C and dark condition, stores the serration Eucheuma muricatum (Gmel.) Web.Van Bos. of different time, at sea from water
Keep hanging cultivation interior fresh weight change in two weeks
Embodiment 5
Sample according to method in embodiment 1, and place serration Eucheuma muricatum (Gmel.) Web.Van Bos. seedling according to embodiment 1, by bubble chamber and Ottelia acuminata (Gagnep.) Dandy
It is placed in 23 ± 1.5 DEG C of temperature environments, or Ottelia acuminata (Gagnep.) Dandy is not carried out moisturizing process, or be placed under high light, after preserving 5 days, stored up
The serration Eucheuma muricatum (Gmel.) Web.Van Bos. deposited or rot or kill, even if some moistening Ottelia acuminata (Gagnep.) Dandy keeps hanging in marine cultivation, does not the most observe Ottelia acuminata (Gagnep.) Dandy
Growth sign occurs, all rots to later stage almost all of Ottelia acuminata (Gagnep.) Dandy.It addition, in 26 DEG C and temperatures above environment, even if in dark,
Moisturizing and under water environment preserve 5 days, Ottelia acuminata (Gagnep.) Dandy keeps hanging after marine, does not also escape the life that almost all of Ottelia acuminata (Gagnep.) Dandy all rots
Fortune.
Can draw from above-mentioned case study on implementation data, serration Eucheuma muricatum (Gmel.) Web.Van Bos. in temperature 17.0-23.0 DEG C, dark and stores from water
Under the conditions of (humidity is maintained at 80 ± 20%), within 5 day time, Ottelia acuminata (Gagnep.) Dandy remains in that vitality.
Claims (2)
1. the method for a serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live, it is characterised in that:
1) serration Eucheuma muricatum (Gmel.) Web.Van Bos. processes from water: by healthy serration Eucheuma muricatum (Gmel.) Web.Van Bos. Sargassum seedling after sea gathers in the crops, drain rapidly surface
Moisture, the degree drained is as the criterion not drip, stand-by；
2) serration Eucheuma muricatum (Gmel.) Web.Van Bos. is placed in after water processes dark in the storage device of 17.0-23.0 DEG C with water suction moisturizing material one
Condition under the relative humidity of 80 ± 20%, puts into the water suction moisturizing material soaking sea water in advance in storage device, containing
The water suction moisturizing material that sea water immersion is crossed is placed in the middle of storage container bottom, surrounding and serration Eucheuma muricatum (Gmel.) Web.Van Bos. or top, and then reaches to prolong
Long storage time and long-time On Transportaion of Live serration Eucheuma muricatum (Gmel.) Web.Van Bos. purpose.
2. the method for the serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live as described in claim 1, it is characterised in that: described water suction moisturizing material
For: Caulis et Folium Lini, Boehmeria, cotton, and the various preferable cloth of chemical fibre water absorption；Soak the water suction moisturizing material humidity after sea water, with
Drip the most outward and be as the criterion.
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| CN201210496811.6A CN103848111B (en) | 2012-11-28 | 2012-11-28 | A kind of serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live the method for seed preservation |
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| CN201210496811.6A CN103848111B (en) | 2012-11-28 | 2012-11-28 | A kind of serration Eucheuma muricatum (Gmel.) Web.Van Bos. On Transportaion of Live the method for seed preservation |
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| CN103848111A CN103848111A (en) | 2014-06-11 |
| CN103848111B true CN103848111B (en) | 2016-12-21 |
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| CN106395029B (en) * | 2016-09-09 | 2019-04-16 | 中国科学院海洋研究所 | A kind of method of asparagus On Transportaion of Live |
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| CN1373633A (en) * | 2000-08-31 | 2002-10-09 | 科学与工业研究委员会 | Improved process for cultivation of algae |
| CN1444870A (en) * | 2003-03-07 | 2003-10-01 | 中国水产科学研究院南海水产研究所 | Processing art for producing instant food contg. Eucheuma |
| CN1608080A (en) * | 2001-12-28 | 2005-04-20 | Cp凱尔科有限公司 | Heterogeneous carrageenan manufacturing process from mono component seaweed with reduced use of level of koh |
| CN201099477Y (en) * | 2007-09-14 | 2008-08-13 | 东莞市农业科学研究中心 | Plant storage box |
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2012
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| CN1373633A (en) * | 2000-08-31 | 2002-10-09 | 科学与工业研究委员会 | Improved process for cultivation of algae |
| CN1608080A (en) * | 2001-12-28 | 2005-04-20 | Cp凱尔科有限公司 | Heterogeneous carrageenan manufacturing process from mono component seaweed with reduced use of level of koh |
| CN1444870A (en) * | 2003-03-07 | 2003-10-01 | 中国水产科学研究院南海水产研究所 | Processing art for producing instant food contg. Eucheuma |
| CN201099477Y (en) * | 2007-09-14 | 2008-08-13 | 东莞市农业科学研究中心 | Plant storage box |
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