CN103843927B - Anti-radiation black tea beverage and preparation method thereof - Google Patents
Anti-radiation black tea beverage and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of processing of beverages and provides an anti-radiation black tea beverage. The anti-radiation black tea beverage is prepared from the following raw materials in parts by weight: 20-80 parts of Tianjian tea, 20-80 parts of Fuzhuan tea A and 20-60 parts of Fuzhuan tea B, wherein the sources of the Fuzhuan tea A and the Fuzhuan tea B are different. A preparation method of the black tea beverage comprises the following steps: (1) uniformly mixing the Tianjian tea, the Fuzhuan tea A and the Fuzhuan tea B; (2) performing extraction at 50-100 DEG C for 30-60 minutes under the condition that the ratio of the tea mixture to water is 1:(10-20); (3) filtering the extracting solution; and (4) blending the filter liquor, and adding edible-grade vitamin C and/or plant sugar. According to the invention, some ingredients against the quality and flavor of the beverage in the raw materials of black tea are removed, not only is the strong pine smoke flavor of original black tea eliminated, but also the black tea beverage is endowed with strong arohid flavor, is mellow and fresh in taste and has lasting aftertaste, and tea liquor is amber and crystal clear. Research proves that the beverage is capable of effectively resisting radiation damage.
Description
Technical field
The invention belongs to drink processing technique field, particularly radiation-resistant black tea drink of one and preparation method thereof.
Background technology
The fast development of China's tea beverage more than ten years of experienced by over, in the market, popular tea beverage has green tea, black tea, also has oolong tea, jasmine tea beverage etc.Comparison is it is well known that black tea and green tea beverage.But black tea drink is almost also commercially be in space state.
The people of NORTHWEST CHINA border ethnic minorities area take in food that is fatty, high protein every day, but rarely have the crowd of obesity.In Tibet, Xinjiang, Qinghai High aititude region, faced by people every day is all strong ultraviolet radiation.Special life and eating habit, harsh climate and ecological environment make black tea become them can not one day or scarce daily necessities.Today, universal along with black tea and human health achievement in research, increasing consumer hankers after product because of prevention inferior health and drinks black tea.But clumsy profile, the packaging being difficult to open are consumed black tea to people and are brought inconvenience.In the epoch of information-based, networking instantly, work and the rhythm of life of people are accelerated day by day, and the time is the wealth that the people that strives in everyone job market is the most luxurious, and the product mode of drinking of traditional black tea allows the desire for consumer goods and the not free prestige tea heart is sighed! Today, we live in the epoch that a radiation is surrounded, and the electromagnetic radiation moment such as computer, TV, mobile phone, microwave, ultraviolet shroud us, and the harm that radiation brings brings huge threat to the health of the especially young colony of people.Radioresistance black tea drink that is convenient, graceful, fashion has huge market potential.
However, the personalized local flavor of tradition black tea pine smoky flavour etc. accepting in the young consumer group is still not high, therefore, and the necessary composition of raw materials by science and advanced processing technology, remove taste flavor unjoyful in black tea, the beverage making it become most consumer to like.The present invention is exactly the technological break-through from aspects such as composition of raw materials, technology, mouthfeel optimization, functional evaluation, and exploitation has conveniently, the radioresistance black tea drink of elegance, healthy characteristic.
Summary of the invention
Not enough for prior art, the object of the invention is to grasp natural, convenient, healthy principle, take into account the assorted technology of raw material and radiation-resisting functional, provide a kind of radiation-resistant black tea drink and preparation method thereof.
For achieving the above object, the invention provides a kind of radiation-resistant black tea drink, pulp furnish is sky point 20-80 part, Fu brick A20-80 part, Fu brick B20-60 part.
Preferred pulp furnish is sky point 20-60 part, Fu brick A20-60 part, Fu brick B25-55 part.
Preferred pulp furnish is sky point 20-40 part, Fu brick A20-40 part, Fu brick B25-50 part.
Preferably, described Fu brick A is different from the tea seedbed of described Fu brick B; Further, the resting period of described Fu brick A and described Fu brick B is more than 1 year.
Fu-brick tea not only has the superior microorganism " golden flower bacterium " (i.e. coronoid process fall apart capsule bacterium) shown unique characteristics, and is promoted the complex transformations of quality component and functional component in Fu-brick tea, define a series of active material with radiation-resisting functional by " growing dim ".Meanwhile, in selected Fu-brick tea, be rich in the radioresistance element of higher than large food 100 ~ 200 times--manganese (average content reaches 110mg/100g), for radiation-resisting functional provides reliable material base.
The invention provides a kind of preparation method of radiation-resistant black tea drink, comprise the following steps,
1) sky point, Fu brick A, Fu brick B are mixed;
2) according to tea-water proportion 1:10-20, under 50-100 DEG C of condition, 30-60min is extracted;
3) extract is filtered;
4) filtrate of gained is allocated, add food grade vitamin C and/or plant sugar.
Preferably, the ascorbic interpolation quality of food grade described in step 4) is 0.001%-0.005%, and the interpolation quality of described plant sugar is 0.01%-0.05%.
Preferably, plant sugar described in step 4) is stevioside.
Compared with prior art, the present invention has the following advantages:
1, this black tea drink is natural, healthy, does not add any other medicinal herb components, is the black tea beverage formed by the optimum organization formula of several dissimilar black tea raw material.
2, by advanced extraction process, efficiently eliminate taste flavor unjoyful in black tea raw material, unsound pine soot fragrance, incorporated strong arohid flavour, flavour is mellow sweet refreshing, long times of aftertaste, and amber soup look glittering and translucent.
3, cytology research shows, this black tea drink has significant radiation resistance.
Accompanying drawing explanation
Fig. 1 Normal group L929 cellular morphology;
Fig. 2 ultraviolet radiation model group L929 cellular morphology;
Fig. 3 black tea drink preincubate group L929 cellular morphology;
Fig. 4 various dose Ultraviolet radiation affects L929 cell survival;
The black tea drink preincubate of Fig. 5 variable concentrations affects Ultraviolet radiation L929 cell OD value.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
A kind of radiation-resistant black tea drink, pulp furnish is sky point 80 parts, Fu brick A20 part, Fu brick B60 part, and the resting period of to be the Gao Jiashan in Anhua, Fu brick B tea seedbed be in the Fu brick A tea seedbed Ma Jiashan in Anhua, Fu brick A is 1 year, and the resting period of Fu brick B is 1 year.
Embodiment 2
A kind of radiation-resistant black tea drink, pulp furnish is sky point 20 parts, Fu brick A80 part, Fu brick B20 part, and Fu brick A tea source is the Ma Jiashan in Anhua, Fu brick B tea source is the Yuntai Shan Mountain in Anhua, and the resting period of Fu brick A is 2 years, and the resting period of Fu brick B is 2 years.
Embodiment 3
A kind of radiation-resistant black tea drink, pulp furnish is sky point 60 parts, Fu brick A60 part, Fu brick B55 part, and Fu brick A tea source is the Gao Jiashan in Anhua, Fu brick B tea source is the Yuntai Shan Mountain in Anhua, and the resting period of Fu brick A is 3 years, and the resting period of Fu brick B is 3 years.
Embodiment 4
A kind of radiation-resistant black tea drink, pulp furnish is sky point 40 parts, Fu brick A40 part, Fu brick B50 part, and Fu brick A tea source is the Yuntai Shan Mountain in Anhua, and Fu brick B tea source is the resting period of the Ma Jiashan in Anhua, Fu brick A is 2 years, and the resting period of Fu brick B is 2 years.
Embodiment 5
A kind of radiation-resistant black tea drink, pulp furnish is sky point 35 parts, Fu brick A35 part, Fu brick B25 part, and the resting period of to be the Ma Jiashan in Anhua, Fu brick B tea source be in the Fu brick A tea source Gao Jiashan in Anhua, Fu brick A is 1 year, and the resting period of Fu brick B is 1 year.
Embodiment 6
A preparation method for radiation-resistant black tea drink, concrete steps are,
1) one-level Dark Green Tea, through screening, assorted, obtain sky point; Fu-brick tea A and Fu-brick tea B, produces tea seedbed difference, all houses more than 1 year time.
2) sky point, Fu brick A, Fu brick B are mixed according to the ratio of 20-80:20-80:20-60.
3) according to tea-water proportion 1:10-20, under 50-100 DEG C of condition, 30-60min is extracted.
4) use filter centrifugal initial filter, collect filtrate, then through inorganic ceramic membrane filtration, preserve filtrate.
5) filtrate of gained is allocated, adds the food grade vitamin C of 0.001%-0.005% and/or 0.01%-0.05% plant sugar, make black tea drink mouthfeel alcohol and, there is arohid flavour, amber soup look, as clear as crystal.
6) sterilizing: whole materials of black tea drink must experience sterilization process in pouring process, as high-temperature short-time sterilization (130-135 DEG C keeps 5S).
7) filling: black tea drink adopts hot filling program, and keeps 10min after full irrigation, then cools.
8) censorship, obtained black tea drink is delivered to quality inspection part and detect, testing result, meets the requirement of GB tea beverage.
The research of embodiment 7 radiation-resisting functional
1 material and instrument
1.1 experiment material
L cell L929 (cellular resources center, Chinese Academy of Sciences Shanghai) Liquid nitrogen storage.
1.2 reagent and instrument and equipment
MEM culture medium (Gibco company of the U.S.); PBS pulvis, Methyl thiazoly tetrazolium assay MTT powder (Amresco company of the U.S.); DMSO, catechin standard items (Sigma Co., USA); Trypsase, hyclone (Hyclone company of the U.S.); Glutathione peroxidase GSH-Px, superoxide dismutase SOD, MDA MDA, hydroxyproline Hyp kit and lactate dehydrogenase L DH (Bioengineering Research Institute is built up in Nanjing); Starorius precision electronic balance (Starorius company of Switzerland); DSY-2-8 water-bath (Beijing Guo Hua medical apparatus and instruments factory); Circulating water type multiplex vavuum pump SHB-B95 type Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.; Ultraviolet specrophotometer (Shimadzu, Japan); Rotary Evaporators (Buchi company of Switzerland); GAMMA1-20 vacuum freeze drier (German Christ company); LC-2010AHT high performance liquid chromatograph (Japanese Shimdzu company); The UV-crosslinked instrument of D8611 pure water preparation instrument (Britain UVP); Superclean bench (Purifying Equipment Co., Ltd., Suzhou); Inverted microscope (German Leica); Multi-functional ELIASA (Thermo company); Centrifuge (German Rotina company); CO
2incubator (U.S. Nuaire); Vibrating machine (its woods Bel instrument manufacturing Co., Ltd of Haimen City); Rotary Evaporators (Buchi company of Switzerland); GAMMA1-20 vacuum freeze drier (German Christ company).
2 experimental techniques
2.1 prepare black tea drink according to the method for embodiment 6
2.2L929 the cultivation of l cell
After recovery, L929 cell is containing 37C DEG C, 5%CO in volume fraction 10% hyclone MEM culture medium
2, Secondary Culture under saturated humidity condition, within every two days, change liquid 1 time, treat that Growth of Cells is in blocks, mutually merge when being paved with 70-80% at the bottom of ware and go down to posterity.
2.3 best ultraviolet radiation dosage choice
Get the L929 cell being in exponential phase, be inoculated in 96 orifice plates with 100 μ L/ holes, every porocyte density is 4 × 104cells/mL, arranges 6 groups, often organizes 6 multiple holes, is placed in 37 DEG C, 5%CO
2cultivate in incubator, cultivate 24h, grow to after 80% fusion until cell, abandon nutrient solution, add appropriate PBS, accept Ultraviolet radiation, dosage is respectively 0,25,50,75,100,125mJ/cm
2, do not irradiate group aluminium foil and cover.Irradiate and completely abandon PBS, add containing 10% hyclone cell culture fluid, put into incubator and continue to cultivate 24h.Then adding 10 μ L concentration to every hole is the MTT of 5mg/mL, hatches 3.5-4h, abandons supernatant, adds 100 μ LDMSO, and put vibration 10min in oscillator, ELIASA measures each hole in 570nm place light absorption value (OD value).Calculate cytoactive: cytoactive=OD experimental group/OD control group × 100%.
Under 2.4 inverted microscopes, cellular morphology is observed
Under Tissue Culture Dish is placed in inverted microscope, observe under cellar culture, UVB irradiate after and add the cell growth status (change such as cellular morphology, size) after the process of black tea drink preincubate, and to take pictures, the change of record cellular morphology.
2.5MTT method measures the black tea drink preincubate of variable concentrations to the impact of cell OD value
Get the cell being in exponential phase and be inoculated in 96 orifice plates, after cell grows to 80%, arrange Normal group, radiation patterns group, black tea drink preincubate group, each group cell all establishes 6 multiple holes.Blank group, radiation patterns group: L929 cell MEM complete culture solution cultivates 24h; Black tea drink preincubate group: give respectively 0 μ g/mL, 2.5 μ g/mL, 5.0 μ g/mL, 10 μ g/mL, 20 μ g/mL, 40 μ g/mL, 80 μ g/mL and a 160 μ g/mL6 concentration black tea drink MEM complete culture solution cultivate 24h.Abandon nutrient solution, add appropriate PBS and cover cell, irradiate, dosage is 100mJ/cm
2, blank group aluminium foil covers.Abandon PBS, add containing 10% hyclone nutrient solution, put into incubator and continue to cultivate 24h.Mtt assay detects cell OD value.
2.6 biochemical colorimetric determination MDA, GSH-Px and SOD content
Getting appropriate cell is inoculated in 12 orifice plates according to the concentration of 2 × 105 cells/well, and cell conditioned medium liquid after collection radiation 24h, requires to operate according to kit description, measure MDA, GSH-Px and SOD content.Often group establishes 3 multiple holes, repeats 5 times, averages.
2.7 data analysis
All in triplicate, result is taken the mean ± standard deviation, and carries out statistical analysis through SPSS17.0forwindows statistical software in experiment.
3 results and analysis
Under 3.1 inverted microscopes, L929 cellular morphology is observed
Observe the larger form of blank group L929 cell under inverted microscope comparatively consistent, arrangement is tight, clear-cut, as shown in Figure 1; And after ultraviolet radiation, cell concentration reduces obviously, between cell, gap becomes large, smaller volume, cell rounding, and floating cells exists in a large number, only deposits respective cells form normally, as shown in Figure 2; But add after 80 μ g/mL black tea drinks hatch, then through the cell number of ultraviolet radiation than radiation group showed increased, and most cells form is normal, sharpness of border, as shown in Figure 3.
Impact on L929 cell survival rate after 3.2 various dose Ultraviolet radiation
See Fig. 4, * under representing same condition of culture, each group is remarkable at P < 0.05 level difference with normal group, * represents that under same condition of culture, respectively group is remarkable at P < 0.01 level error heteropole with normal group, under identical condition of culture, along with the increase of dose of radiation, cell survival rate declines.Compared with the cell without ultraviolet radiation, when giving 25mJ/cm
2during dose of radiation, cytoactive does not have influenced substantially, without conspicuousness, and gives 50 and 75mJ/cm
2during dose of radiation, cytoactive declines comparatively obvious, has significant difference (P < 0.05), but when dose of radiation is 100 and 125mJ/cm
2time, cytoactive obviously declines, and have pole significant difference (P < 0.01), cell normal growth is obviously suppressed, therefore this experimental selection 100mJ/cm
2dose of radiation is used for follow-up study.
The black tea drink preincubate of 3.3 variable concentrations affects the OD value of L929 cell after irradiated cell intervention
Known by MTT detection method, under same condition of culture, OD value is directly proportional to cell survival rate, and under representing same condition of culture see Fig. 5, *, each group is remarkable at P < 0.05 level difference with normal group, through 100mJ/cm
2after dose of radiation irradiates, when the concentration of black tea drink preincubate is 2.5-5.0 μ g/mL, the change of OD value is less, little with model group difference, and when the concentration of black tea drink preincubate is 10-80 μ g/mL, OD value strengthens with the increase of black tea drink concentration, and has significant difference (P < 0.05).Therefore show when the concentration of black tea drink preincubate is for effectively suppressing the cell death caused by ultraviolet radiation damage during 10-80 μ g/mL.But when black tea drink preincubate dosage is 160 μ g/mL, there is decline in OD value, illustrates that excessive dosage has occurred negatively influencing to cell survival rate to a certain extent.
3.4 biochemical colorimetric method calibrating MDA, GSH-Px and SOD content
Black tea drink concentration is selected to be 10,20,40 and 80 μ g/mL, with biochemical colorimetric method calibrating MDA, GSH-Px and SOD content.Result is as shown in table 1, compared with Normal group, gives 100mJ/cm
2the vigor of the main antioxidant enzymes such as the model control group cell SOD of Ultraviolet radiation and GSH-Px significantly reduces, and MDA content significantly raises, and the two difference has statistical significance (P < 0.01), shows 100mJ/cm
2radiation causes the normal oxidation resistance damage of L929 cell serious.
On the contrary, through the variable concentrations nutrient solution preincubate 24h of black tea drink, in the black tea drink preincubate group L929 cell accepting Ultraviolet radiation again, the content of main antioxidant enzyme reduces and obtains obvious containment, and all along with the increase of preincubate concentration, the content of main antioxidant enzyme increases, and presents certain dosage effect, compared with model control group, have significant difference (P < 0.05), especially the high dose group of 80 μ g/mL has a significant effect.The oxidative damage that black tea drink can be alleviated ultraviolet radiation and causes cell is described.
Table 1 black tea drink variable concentrations is on the impact of Ultraviolet radiation L929 cell SOD, GSH-Px vigor and MDA content
Note:
*under representing same condition of culture, each group is remarkable at P < 0.01 level error heteropole with normal group.## represents that under same condition of culture, respectively group is remarkable at P < 0.01 level error heteropole with model group
4 conclusions
This research sets up external radiation model by ultraviolet B radiation inducing mouse fibroblast (L929) cellular damage, and research black tea drink is to the protective effect of L929 cell after Ultraviolet radiation.Ultraviolet radiation promotes that body produced polyradical, makes cell tissue be subject to the oxidative stress of the free radicals such as active oxygen, thus causes damage, works as 100mJ/cm
2after Ultraviolet radiation L929 cell, in cytoactive and cell, main antioxidase content significantly reduces, and lipid peroxidation product MDA (MDA) content significantly raises, but after giving black tea drink finite concentration process 24h in advance, in cell survival rate and cell antioxidase comparatively radiation patterns group have remarkable lifting, MDA (MDA) content is starkly lower than untreated radiation patterns group simultaneously.As can be seen here, the l cell of black tea drink to UVB radiation injury has protection and mitigation, can suppress the radiation-induced cell oxidative damage of UVB and apoptosis to a certain extent, strengthen the content of main antioxidant enzyme in its cell.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (5)
1. a radiation-resistant black tea drink, is characterized in that, pulp furnish is sky point 20-60 part, Fu brick A20-60 part, Fu brick B25-55 part, and wherein, described Fu brick A is different from the tea seedbed of described Fu brick B; The resting period of described Fu brick A and described Fu brick B is more than 1 year;
Described radiation-resistant black tea drink is prepared from by the method comprised the following steps:
1) sky point, Fu brick A, Fu brick B are mixed;
2) according to tea-water proportion 1:10-20, under 50-100 DEG C of condition, 30-60min is extracted;
3) extract is filtered;
4) filtrate of gained is allocated, add food grade vitamin C and/or plant sugar.
2. radiation-resistant black tea drink according to claim 1, is characterized in that, pulp furnish is sky point 20-40 part, Fu brick A 20-40 part, Fu brick B 25-50 part, and wherein, described Fu brick A is different from the tea seedbed of described Fu brick B.
3. a preparation method for radiation-resistant black tea drink as claimed in claim 1 or 2, is characterized in that, comprise the following steps,
1) sky point, Fu brick A, Fu brick B are mixed;
2) according to tea-water proportion 1:10-20, under 50-100 DEG C of condition, 30-60min is extracted;
3) extract is filtered;
4) filtrate of gained is allocated, add food grade vitamin C and/or plant sugar.
4. preparation method according to claim 3, is characterized in that, step 4) described in the ascorbic interpolation quality of food grade be 0.001%-0.005%, the interpolation quality of described plant sugar is 0.01%-0.05%.
5. the preparation method according to claim 3 or 4, is characterized in that, step 4) described in plant sugar be stevioside.
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| CN114831197B (en) * | 2022-05-23 | 2023-08-29 | 湖南楚茗茶业有限公司 | Composite black tea and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101617846A (en) * | 2009-07-30 | 2010-01-06 | 铁生年 | Tuckahoe tea beverage |
| CN103083535A (en) * | 2012-12-10 | 2013-05-08 | 贺志弘 | Dark tea composition and application in weight losing field |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101617846A (en) * | 2009-07-30 | 2010-01-06 | 铁生年 | Tuckahoe tea beverage |
| CN103083535A (en) * | 2012-12-10 | 2013-05-08 | 贺志弘 | Dark tea composition and application in weight losing field |
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