CN103843824B - A kind ofly improve seed soaking agent of kitchen garden salt resistance and preparation method thereof - Google Patents
A kind ofly improve seed soaking agent of kitchen garden salt resistance and preparation method thereof Download PDFInfo
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- CN103843824B CN103843824B CN201310618019.8A CN201310618019A CN103843824B CN 103843824 B CN103843824 B CN 103843824B CN 201310618019 A CN201310618019 A CN 201310618019A CN 103843824 B CN103843824 B CN 103843824B
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Abstract
The present invention a kind ofly improves seed soaking agent of kitchen garden salt resistance and preparation method thereof.The formula of its raw material is: calcium chloride 10-15mmol/L; Salicylic acid 10-15mmol/L; 5-ALA: 0.15-0.20mg/L, trace element: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na
2moO
40.10mg/L, CuSO
40.030mg/L, all the other are distilled water.The germination rate of kitchen garden seed under salt stress process can be improved under this seed soaking agent process, strengthen kitchen garden seedling to the resistivity of salt stress.
Description
Technical field
The present invention relates to field of plant growing technology, specifically a kind ofly improve seed soaking agent of kitchen garden salt resistance and preparation method thereof.
Background technology
Kitchen garden (PortulacaoleraceaL.) belongs to Portulacaceae Portulaca, is one of traditional medicinal and edible plant of China, is distributed widely in temperate zone, the world and torrid areas.The composition such as rich polysaccharose, flavonoids, unsaturated fatty acid, alkaloids, terpene in kitchen garden cauline leaf, has the effects such as antibacterial, antiviral, reducing blood lipid, antiatherosclerosis.The kitchen garden product developed in the market has drinks, medicine tablet, injection, oral liquid, compound purslane lotion etc.Kitchen garden plant has certain salt resistance, is the important plant resources that China coast beach and saline land, inland develop.But due to kitchen garden germination period and seedling stage more responsive to salt stress, under middle high salinity, seed germination is suppressed, and therefore on the saline land of salinity inequality, commerial growing occurs shoot-breaking phenomenon of being short of seedling often.Improve kitchen garden germination period and the salt resistance in seedling stage, for realize kitchen garden the culture and utility in saline and alkaline beach area and the exploitation in saline and alkaline soil significant.
Research shows, seed soaking agent can promote the sprouting of plant seed, improves seed activity and emergence rate, strengthens anti-cold, the drought resisting of plant seedlings, the resistance such as disease-resistant, obtains higher Production Gain.The seed soaking agent of current use mainly contains drought resisting, anti-cold, disease-resistant type etc., salt-resistant type seed soaking agent is still rare, and the research at present for salt-resistant type seed soaking agent launches mainly for one-component allogenic material, for the research of multiple allogenic material synergistic function, there is not been reported.
The compound Drought-resistant wheat seed soaking agent that Jia Hongtao etc. (2010) develop, the wheat seeds sprouting rate of seed soaking agent process, germination vigor raises, seedling radical and root length all significantly increase, seedling overground part dry weight, water retention capability and MDA content are lower than contrast, root dry weight, root/shoot ratio, chlorophyll content, stomatal conductance, Net Photosynthetic Rate, nitrate reductase activity, catalase activity is all apparently higher than contrast, illustrate that the process of compound Drought-resistant seed soaking agent significantly can increase wheat roots growth under drought stress, anti-oxidant, Nitrate-N Use and photosynthetic performance, seed soaking agent also promotes seed germination, thus the injury that reduction drought stress causes seedling, improve Drought-resistance in Wheat ability
[1].Sun little Fang etc. (2000) adopt quartz sand Hydroponics, and research mepiquat chloride (DPC) and calcium chloride solution seed soaking are to the regulating action of Seed germination and emergence phase cotton plant salt resistance.Result shows, under 150mmol/LNaCl coerces, 0.5mg/LDPC and 10mmol/LCaCl
2two kinds of seed soakings can alleviate the negative effect of salt stress to the cotton seedling dry matter of cotton germination gesture, emergence rate and two leaf stage
[2].Agricultural University Of South China succeeds in developing " neat seedling is fast " seed soaking agent, after seed soaking agent process, the planting percent of Hunan early No. 24, Xian and excellent No. 2 of strain two improves 36.61% and 33.57% than contrast respectively, significantly reduce the plant height of rice seedling, increase leaf age and unit height of seedling dry mass, reduce membrane permeability and malonaldehyde (MDA) content of rice leaf
[3].The disease-resistant type seed soaking agent of beet of Chinese Academy of Agricultural Sciences's beet Research Institute, resistance in sugarbeet characteristic of disease through seed soaking agent process obviously strengthens, and damping off does not occur, and root rot morbidity is 0.01%, comparatively contrast reduction by 0.07%, enhance the resist ability to damping off and root rot
[4].
Existing seed soaking agent technology is mainly used in the crops such as paddy rice, wheat, corn and beet, and application report in kitchen garden produces is less, in the sprouting of kitchen garden, the growing of seedling, also have no report.
Goal of the invention:
The object of this invention is to provide and a kind ofly improve seed soaking agent of kitchen garden salt resistance and preparation method thereof, the sprouting of seed under salt stress can be promoted, improve the salt resistance of seedling, strengthen seed, seedling to the adaptation of salt stress, thus obtain higher germination rate and survival rate of seedling.
Technical scheme of the present invention is:
Seed soaking agent of the present invention comprises: calcium chloride 10-15mmol/L; Salicylic acid 10-15mmol/L; 5-ALA: 0.15-0.20mg/L, trace element: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na
2moO
40.10mg/L, CuSO
40.030mg/L, all the other are distilled water.
Preparation method: 1. take calcium chloride and be dissolved in distilled water; 2. take the absolute ethyl alcohol that salicylic acid is first dissolved in trace, then mix with solution above; 3. taking 5-ALA is dissolved in distilled water, now with the current, mixes during use with previous section; 4. trace element is made into mother liquor; 5. last distilled water is settled to the volume of required preparation.The raw materials used in use noresidue of seed soaking agent of the present invention, pollution-free, can seed germination be promoted, strengthen kitchen garden seedling salt resistance ability that there is volume increase, the advantage such as efficient, inexpensive.
Accompanying drawing explanation
Fig. 1 represents the impact of salicylic acid, calcium chloride, 5-ALA combination seed soaking on kitchen garden seed germination rate under 150mmol/LNacl Stress treatment.
Fig. 2 represents the impact of salicylic acid, calcium chloride, 5-ALA combination seed soaking on kitchen garden seedling Electrolytic leakage under 180mmol/LNacl Stress treatment.
Fig. 3 represents the impact of salicylic acid, calcium chloride, 5-ALA combination seed soaking on kitchen garden seedling malonaldehyde (MDA) content under 180mmol/LNacl Stress treatment.
Fig. 4 represents seed soaking agent seed soaking (left side), kitchen garden the emerging and growing state under 180mmol/LNacl Stress treatment on soak seed without seed soaking (centre) and distilled water (right side)
Fig. 1, Fig. 2, Fig. 3 respectively fill a prescription medium trace element composition identical: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na
2moO
40.10mg/L, CuSO
40.030mg/L, other constituent concentration is as follows:
Formula A: calcium chloride 10mmol/L; Salicylic acid 10mmol/L; 5-ALA: 0.15mg/L;
Formula B: calcium chloride 10mmol/L; Salicylic acid 15mmol/L; 5-ALA: 0.15mg/L;
Formula C: calcium chloride 10mmol/L; Salicylic acid 10mmol/L; 5-ALA: 0.20mg/L;
Formula D: calcium chloride 10mmol/L; Salicylic acid 15mmol/L; 5 amino-laevulic acids: 0.20mg/L;
Formula E: calcium chloride 15mmol/L; Salicylic acid 10mmol/L; 5-ALA: 0.15mg/L;
Formula F: calcium chloride 15mmol/L; Salicylic acid 15mmol/L; 5-ALA: 0.15mg/L;
Formula G: calcium chloride 15mmol/L; Salicylic acid 10mmol/L; 5-ALA: 0.20mg/L;
Formula H: calcium chloride 15mmol/L; Salicylic acid 15mmol/L; 5-ALA: 0.20mg/L;
Embodiment
Embodiment 1:
The composition of raw materials of seed soaking agent is: salicylic acid 15mmol/L; Calcium chloride 15mmol/L; 5-ALA: 0.15mg/L, trace element: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na
2moO
40.10mg/L, CuSO
40.030mg/L, all the other are distilled water.
Preparation method: 1. take calcium chloride and be dissolved in distilled water; 2. take the absolute ethyl alcohol that salicylic acid is first dissolved in trace, then mix with solution above; 3. taking 5-ALA is dissolved in distilled water, now with the current, mixes during use with previous section; 4. trace element is made into mother liquor; 5. last distilled water is settled to the volume of required preparation.
Effect:
Under this seed soaking agent process, the germination rate of seed is significantly improved under salt stress, as shown in Figure 1, under 150mmol/LNacl Stress treatment condition, its germination rate improves nearly 18.1% than contrast, difference reaches significantly (P < 0.05), illustrates that the seed soaking of this seed soaking agent can improve the germination rate of kitchen garden seed under Nacl Stress treatment.
Embodiment 2:
The composition of raw materials of seed soaking agent is: salicylic acid 10mmol/L; Calcium chloride 10mmol/L; 5-ALA: 0.20mg/L, trace element: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na
2moO
40.10mg/L, CuSO
40.030mg/L, all the other are distilled water.
Preparation method: 1. take calcium chloride and be dissolved in distilled water; 2. take the absolute ethyl alcohol that salicylic acid is first dissolved in trace, then mix with solution above; 3. taking 5-ALA is dissolved in distilled water, now with the current, mixes during use with previous section; 4. trace element is made into mother liquor; 5. last distilled water is settled to the volume of required preparation.
Effect:
Under this seed soaking agent process, alleviate six injuries of leaf phase seedling after 180mmol/LNacl coerces 7 days continuously, Electrolytic leakage in root and blade reduces 18.5% and 14.6% than contrast respectively, MDA content in root, leaf then reduces 18.3 μm of ol/gFW, 10.2 μm of ol/gFW respectively, significant difference (P < 0.05), as shown in Figure 2 and Figure 3.
Above result shows, can improve the germination rate of seed under normal sprouting, salt stress process, and improve seedling to the resistivity of salt stress under this seed soaking agent process.
Bibliography:
[1] Jia Hongtao, Cao Shandong, Du Lishu. the Primary Study [J] of wheat special compound Drought-resistant seed soaking agent and seed coating medicine drought resistance mechanism. wheat crops journal, 2010,30 (3): 496-499
[2] Sun little Fang, Zheng Qingsong, Liu Youliang. mepiquat chloride and the adjustment of calcium chloride seed soaking to Seed germination and emergence phase cotton plant salt resistance. Jiangsu's agriculture journal, 2000,16 (4): 204-207
[3] Li Wu, Luo Xiwen, Li Guoxi, etc. seed soaking agent and the impact [J] of irrigating Direct-seeding Rice seedling quality under Seedling Stage artificial hypothermia. North China agronomy report, 2010,25 (2): 224-238
[4] Wang Yuewu, Liu Hongbo, Sun Tiezhu. application seed soaking agent is on the impact [J] of beet yield and quality. Chinese carbohydrate, 2000,2:34-35.
Claims (2)
1. improve a seed treatment for kitchen garden salt resistance, it is characterized in that the formula of its raw material is: calcium chloride 10-15mmol/L; Salicylic acid 10-15mmol/L; 5-ALA: 0.15-0.20mg/L, trace element: MnSO
426.0mg/L, ZnSO
47.0mg/L, Na2MoO
40.10mg/L, CuSO
40.030mg/L, all the other are distilled water.
2. a kind of preparation method improving the seed treatment of kitchen garden salt resistance according to claim 1: it is characterized in that 1. taking calcium chloride is according to the following steps dissolved in distilled water; 2. take the absolute ethyl alcohol that salicylic acid is first dissolved in trace, then mix with solution above; 3. taking 5-ALA is dissolved in distilled water, now with the current, mixes during use with previous section; 4. trace element is made into mother liquor; 5. last distilled water is settled to the volume of required preparation.
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| CN104961545B (en) * | 2015-06-10 | 2017-10-13 | 江苏沿海地区农业科学研究所 | A kind of raising salt-soda soil sugar grass emerges the preparation of quality |
| CN106472137A (en) * | 2016-09-19 | 2017-03-08 | 江苏省农业科学院 | A kind of method improving Salt Tolerance in Rice Seedlings |
| CN106614740A (en) * | 2016-12-26 | 2017-05-10 | 江苏沿海地区农业科学研究所 | Seed treating agent for enhancing salt tolerance of brassica napus and preparation method of seed treating agent |
| CN107087641B (en) * | 2017-02-08 | 2021-04-13 | 福建三炬生物科技股份有限公司 | Marine oligosaccharide biological preparation for improving salt resistance of crops and preparation method thereof |
| CN108276208A (en) * | 2018-01-31 | 2018-07-13 | 潍坊友容实业有限公司 | A kind of grass seed seed soaking agent and its application method |
| CN112136831B (en) * | 2020-09-16 | 2021-08-03 | 南开大学 | Method for increasing plant salt resistance efficiency by self-synthesizing crystal |
| CN115246678A (en) * | 2021-10-13 | 2022-10-28 | 青岛大学 | Constructed wetland system with high salt tolerance |
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|---|---|---|---|---|
| US5661111A (en) * | 1994-11-28 | 1997-08-26 | Cosmo Research Institute | Method for improving plant salt-tolerance |
| CN1290483A (en) * | 1999-09-30 | 2001-04-11 | 山东师范大学 | Anti-salt drought resistant agent for plant and its using method |
| CN102168106A (en) * | 2010-12-20 | 2011-08-31 | 南京农业大学 | Transgenic method capable of controlling ALA synthesis in plants and promoting growth and stress resistance |
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2013
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5661111A (en) * | 1994-11-28 | 1997-08-26 | Cosmo Research Institute | Method for improving plant salt-tolerance |
| CN1290483A (en) * | 1999-09-30 | 2001-04-11 | 山东师范大学 | Anti-salt drought resistant agent for plant and its using method |
| CN102168106A (en) * | 2010-12-20 | 2011-08-31 | 南京农业大学 | Transgenic method capable of controlling ALA synthesis in plants and promoting growth and stress resistance |
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