CN103843778A

CN103843778A - Bactericidal composition containing tetraconazole

Info

Publication number: CN103843778A
Application number: CN201210515629.0A
Authority: CN
Inventors: 毕玉轩
Original assignee: Qingdao Baicaohui Institute of Chinese Herbal Medicine
Current assignee: Qingdao Baicaohui Institute of Chinese Herbal Medicine
Priority date: 2012-12-05
Filing date: 2012-12-05
Publication date: 2014-06-11

Abstract

The invention relates to a compound bactericide. The effective components of the compound bactericide are tetraconazole and boscalid, wherein the mass percent ratio of tetraconazole to boscalid is (1-80): (80-1), and the content of tetraconazole and boscalid in a compound preparation is 1-80%. The compound bactericide provided by the invention can be prepared into such dosage forms as missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion or the like by a method known to those skilled in the art. The bactericidal composition provided by the invention can be used for preventing and controlling fungous diseases and bacterial diseases of a variety of crops, such as powdery mildew, gray mold, rice blast, a variety of rots and root rot and the like, and the bactericidal composition is environment-friendly and has good synergism.

Description

A kind of bactericidal composition that contains tetraconazole

Technical field

The present invention relates to a kind of composite bactericide of pesticide field, its active ingredient is tetraconazole and Boscalid, and this composite control for multiple diseases has synergistic effect.

Background technology

Tetraconazole (tetraconazole), chemical name is: 2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl) propyl group 1,1,2,2-tetrafluoro ethyl ether.Tetraconazole belongs to sterol demethylation inhibitor, and this medicine is mainly the biosynthesis that destroys and organize the cell membrane important composition composition ergosterol of germ, causes cell membrane not form, and makes germ death.Due to absorption in having well, therefore can be absorbed by plants rapidly, and in conducted inside, there is good protection and therapeutic action.And the lasting period is long.

Boscalid is the new nicotinamide bactericide of BASF Aktiengesellschaft's exploitation.Belong to succinic acid ubiquinone reductase inhibitor in mitochondrial respiratory chain, the sprouting of spore is had to very strong restraint, and Boscalid and other bactericide do not have cross resistance.Boscalid has good control efficiency for powdery mildew, gray mold, rice blast, various rot, brown rot and root rot.

summary of the invention:

Because weather is comparatively unusual in recent years, Changes in weather is too fast, causes corps diseases serious, and too much dispenser is that various sterilization agent produces resistance, in view of the situation, invents a kind of microbicide compositions with synergistic effect and delaying drug resistance appearance.This bactericidal composition comprises active ingredient tetraconazole and Boscalid.Be used for preventing and treating the diseases such as powdery mildew, gray mold, rice blast and various rots.

Bactericidal composition of the present invention is characterised in that, the mass ratio of active ingredient tetraconazole and Boscalid is 1 ~ 80:80 ~ 1.

Bactericidal composition of the present invention is characterised in that, measures through virulence test, and active ingredient tetraconazole is 1 ~ 50:50:1 with the quality optimization ratio of Boscalid

The type that bactericidal composition of the present invention can be prepared according to the known method of the art personnel comprises the formulations such as missible oil, suspending agent, wetting powder, water dispersible granules, aqueous emulsion, microemulsion.

In described bactericidal composition, tetraconazole and Boscalid gross mass content are 1% ~ 80%, preferably than being 5% ~ 60%.

Bactericidal composition of the present invention has obvious synergistic effect, can reduce the consumption of active ingredient, delays the generation of disease for bactericide resistance.Have prevention and protective effect concurrently, tetraconazole can produce obvious production-increasing function after dispenser simultaneously.

Bactericidal composition of the present invention is applicable to fungal disease and the bacterial diseases such as powdery mildew, gray mold, rice blast, various rot, brown rot and the root rot in multiple kinds of crops.

Embodiment:

For making technical solution of the present invention clearer, below be illustrated with specific embodiment, but the present invention is not limited only to these examples.

Different farm chemical ingredients being carried out composite, is to solve at present some in using of some single doses comparatively effectively and mode efficiently.The agricultural of different types of structure is carried out composite conventionally there will be afterwards three kinds of type of action, i.e. antagonism, summation action and synergistic effect.But being specially which kind of type of action only has by great many of experiments and can learn.

The present invention carries out composite with mitochondrial respiratory inhibitor tetraconazole and Boscalid, they have obvious synergistic effect for some diseases at quality inspection combination, and are not only that two kinds of medicaments obtain simply addition.Test and be illustrated below by raw test.

tetraconazole proportioning joint toxicity measurings different from Boscalid

one, subjects: rice blast (Pyricularia oryzae Cav)

This test adopts pot-culture method, selects paddy rice to infect rice blast seed, and after vernalization, 20 of every basin sowings, grew to for three one heart stages of leaf, selects the rice seedlings numbering that growing way is consistent for subsequent use.According to medicament activity, 5 ~ 7 series mass concentration are set.Liquid is evenly sprayed in blade face to all moistening, for subsequent use after liquid natural air drying.Every processing 5 basins, and establish the processing that only contains solvent and surface-active and do not contain active ingredient as blank.After chemicals treatment 24h, on rice leaf, evenly spray inoculating spores suspension with sprayer.The collocation method of spore suspension is as follows: Pyricularia oryzae is cultured on suitable medium and produces a large amount of spores, with spore under aseptic washing, after filtration, making concentration is 1x10 ⁵the spore suspension of individual spore/mL.Dark moisturizing (relative moisture 100%, 25 ~ 26 DEG C of temperature) after inoculation, (humidity maintains 90% left and right) cultivated in 24 as a child illumination moisturizings, after inoculation 7d, takes out.Add up the scab number on every strain rice leaf (the 1st, 2,3 leaf) and carry out classification record, calculating disease index and control efficiency.

Rice blast grade scale:

0 grade: anosis;

1 grade: occur brown some scab;

3 grades: occur typical spindle scab, lesion area accounts for whole leaf area below 5%;

5 grades: typical scab, lesion area accounts for whole leaf area 6 ~ 25%;

7 grades: typical scab, lesion area accounts for whole leaf area 26 ~ 50%;

9 grades: typical scab, lesion area accounts for whole leaf area more than 50%.

Table 1 drug effect computational methods:

Control efficiency is converted into probability value (y), and liquor strength (μ g/mL) converts logarithm value (X) to, calculates concentration EC in virulence equation and inhibition with method of least squares ₅₀, according to toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.

Actual measurement toxicity index (ATI)=(standard medicament EC ₅₀/ reagent agent EC ₅₀) × 100

The percentage composition co-toxicity coefficient (CTC) of B in percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture=[the theoretical toxicity index of mixture actual measurement toxicity index (ATI)/mixture (TTI)] × 100

Evaluation criterion≤80, composition performance is antagonism; 80 < CTC < 120, composition performance is summation action; CTC >=120, composition performance is synergistic effect.

The pot experiment measurement result of the serial proportioning of table 2 tetraconazole and Boscalid to rice blast

Process	EC ₅₀（μg/mL）	ATI	TTI	Co-toxicity coefficient CTC
					Tetraconazole	4.82	100	——	——
Boscalid	1.61	299.4	——	——
					Tetraconazole 20:1 Boscalid	2.22	217.1	109.5	198.3
Tetraconazole 10:1 Boscalid	1.93	249.7	118.1	211.4
					Tetraconazole 5:1 Boscalid	1.63	295.7	133.2	222.0
Tetraconazole 1:1 Boscalid	0.98	491.8	199.7	246.3
					Tetraconazole 1:5 Boscalid	0.78	617.9	266.1	232.2
Tetraconazole 1:10 Boscalid	0.81	595.1	281.3	211.6
					Tetraconazole 1:20 Boscalid	0.87	554.0	289.9	191.1

Result of the test shows, the composite control rice blast of tetraconazole and Boscalid, proportioning is between 1 ~ 20:20 ~ 1 time, co-toxicity coefficient is all more than 120, all show as synergistic effect, and in the time that tetraconazole and Boscalid mass ratio are between 1 ~ 10:10 ~ 1, co-toxicity coefficient is all more than 200, synergistic effect is more obvious, better aspect the effect of control rice blast accordingly.

two, experimental subjects: powdery mildew of cucumber (Sphaerotheca fuliginea)

This test adopts pot-culture method.The cucumber variety of sowing sense powdery mildew, chooses the cucumber seedling that grows to 2 leaf periods, and every processing 5 basins, number for subsequent use.On the basis of preliminary experiment, according to active these 5 ~ 7 the series mass concentration of medicament.Liquid is evenly sprayed to blade face to all moistening, treats that medicine company natural air drying is for subsequent use.And establish the processing that only contains solvent and surface-active and do not contain active ingredient as blank.24h after dispenser, inoculation powdery mildew spore suspension.Spore suspension preparation method is as follows: get and cover with Powdery Mildew cucumber leaves, use fresh spore under the pure washing that is added with a small amount of surface reactive material, making concentration is 1x10 ⁵the spore suspension of individual spore/ml.In greenhouse, cultivate after 7d, record the lesion area on every strain cucumber seedling and add up, calculate disease index and control efficiency.

Powdery mildew of cucumber grade scale

0 grade: anosis;

1 grade: sorus area accounts for below 5% of whole leaf area;

3 grades: sorus area accounts for below 6 ~ 10% of whole leaf area;

5 grades: sorus area accounts for below 11 ~ 20% of whole leaf area;

7 grades: sorus area accounts for below 21 ~ 50% of whole leaf area;

9 grades: sorus area accounts for the more than 5% of whole leaf area.

Table 3 drug effect computational methods

The serial proportioning of table 4 tetraconazole and Boscalid is to powdery mildew of cucumber pot experiment measurement result

Process	EC ₅₀（μg/mL）	ATI	TTI	Co-toxicity coefficient CTC
					Tetraconazole	4.79	100	——	——
Boscalid	1.31	365.6	——	——
					Tetraconazole 20:1 Boscalid	2.13	224.9	112.6	199.6
Tetraconazole 10:1 Boscalid	1.73	276.9	124.1	223.0
					Tetraconazole 5:1 Boscalid	1.33	360.2	144.3	249.6
Tetraconazole 1:1 Boscalid	0.78	614.1	232.8	263.8
					Tetraconazole 1:5 Boscalid	0.62	772.6	321.4	240.4

Result of the test shows, the composite powdery mildew of cucumber of preventing and treating of tetraconazole and Boscalid, and proportioning all has synergistic effect within said ratio, is that 1:1 is that co-toxicity coefficient is 263.8 in proportioning, and synergistic effect is the most obvious.

Known by above test, the present invention has good synergistic effect for plant disease within said ratio.Be suitable for the multiple diseases of cereal, fruits and vegetables.Greatly reduced pesticide dosage, significant for environmental protection, toxicity is low simultaneously, suitable for producing green agricultural product.

Claims

1. taking tetraconazole and Boscalid as a composite bactericide for active ingredient, it is characterized in that: the mass ratio of tetraconazole and Boscalid is 1 ~ 80:80 ~ 1.

2. composite bactericide according to claim 1, is characterized in that: active ingredient tetraconazole is 1 ~ 50:50 ~ 1 with the quality optimization ratio of Boscalid.

3. composite bactericide according to claim 1, is characterized in that: active ingredient tetraconazole and the Boscalid total mass ratio in composition is 1 ~ 80%.

4. composite bactericide according to claim 1, is characterized in that: active ingredient tetraconazole with Boscalid the gross mass in composition preferably than being 5 ~ 60%.

5. composite bactericide according to claim 1, is characterized in that: adopt the known technical scheme of those skilled in the art can be prepared into the formulations such as missible oil, suspending agent, wetting powder, water dispersible granules, aqueous emulsion, microemulsion.

6. composite bactericide according to claim 1, is characterized in that: bactericidal composition of the present invention is applicable to fungal disease and the bacterial disease in multiple kinds of crops.