CN103837509A - Time difference type light source apparatus for fluorescence detection, image capture system and capture method - Google Patents
Time difference type light source apparatus for fluorescence detection, image capture system and capture method Download PDFInfo
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- CN103837509A CN103837509A CN201210483558.0A CN201210483558A CN103837509A CN 103837509 A CN103837509 A CN 103837509A CN 201210483558 A CN201210483558 A CN 201210483558A CN 103837509 A CN103837509 A CN 103837509A
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Abstract
The invention relates to a time difference type light source apparatus for fluorescence detection, an image capture system and a capture method, wherein a set of pulse exciting light source assemblies in the light source apparatus is mainly adopted, and is matched with a set of shutters to capture a photoluminescence image of an object requiring detection and arranged on a predetermined position requiring detection. The time difference type light source apparatus for fluorescence detection comprises: a set of pulse exciting light source assemblies emitting light to the predetermined position requiring detection, and a set of controllers for instructing the pulse exciting light source assemblies to emit light and feeding back the signal to connect the shutters. According to the present invention, the shutters are closed when the pulse exciting light source assemblies emit the light, and the shutters are opened when the light emitting of the pulse exciting light source assemblies is completed so as to shield the reflection and diffuse reflection light beams and simply capture the photoluminescence data.
Description
[technical field]
The invention relates to a kind of light supply apparatus, image acquisition system and acquisition method thereof, the method for time difference type light supply apparatus, image acquisition system and acquisition photoluminescence image that especially a kind of detection photoluminescence (photoluminescence) image data is used.
[background technology]
Along with the progress of optical technology, make the difference between various video camera, camera, camera cell phone fuzzy gradually, even general camera cell phone also can be known and take pictures; In addition; because the application of fluoroscopic image is in recent years tending towards extensive; therefore the device that possesses fluoroscopic image acquisition function also receives publicity gradually; in practical application; fluoroscopic image is except be used in ecological observation and scientific research in the past; recently more extend to medical and beauty treatment, criminal identification or anti-counterfeiting identification, as: thereby identification of fingerprint, bill anti-counterfeiting identification, even the fluorescin detected in body fluid is searched bloodstain or body fluid.
In general, photoluminescence video picture is mainly that the incident light of a higher-frequency is irradiated and had on the determinand of fluorescence (fluorescence) or phosphorescence (phosphorescence) characteristic, make to have in determinand for example green fluorescent protein of molecule (the Green Fluorescence Protein of fluorescence or phosphorescent characteristics, GFP), absorbing after the photon excitation of for example 365nm ultraviolet light range, in the time that the electronics being excited in molecule is got back to ground state by the transition of high-energy orbital, just can discharge lower frequency fluorescence or the phosphorescence of for example 525nm green glow.
But in the process due to photoluminescence, the luminous bundle of excitation source, most absorption by fluorescence molecule, is directly reflected or diffuse reflection but expose to determinand surface; That is to say, catoptrical brightness is normally with thousand times of fluorescence that even ten thousand times of ground discharges higher than determinand, even if wherein only have for example centesimal light beam of small part to enter photo-sensitive cell, also by making the signal of above-mentioned photoluminescence image be subject to severe jamming because of reflection or irreflexive noise light, cause the difficulty in observation.
Further consider the interference of existing veiling glare in environment, for example be in the strong scene of sunlight, while looking for the bloodstain that is dispersed in ground, if irradiate everywhere with artificial ultraviolet light source, to observe according to this photoluminescence reaction of bloodstain, and figure for the position of bloodstain, and be also easy to be covered by the sunburst of environment and seriously erroneous judgement, significantly ignore fluorescence or the phosphorescence mark card of necessary being.
So relevant criminal identification, biology or Physiologic Studies, or medical cosmetology field personage, invariably wish that reflection source or extraneous veiling glare energy and photoluminescence image data separate to some extent, and the unlikely photoluminescence information that affects, as shown in Figure 1, existing a kind of secondary light source 9 is luminous by the lamp source of ultraviolet light, and via interior snake belly tube 91 leaded lights that are embedded with optical fiber, user can curve snake belly tube 91 voluntarily to suitable angle, allow light source irradiate determinand to approach lateral direction light emission, the direct reflected light of luminous bundle just can not return to immediately camera lens place and disturb fluorescence or phosphorescence image.
But the structure of this adjustment light-source angle, due to the restriction of snake belly tube 91, the bending angle of snake belly tube 91 is difficult for accurate adjustment on the one hand, illumination source, another aspect number only has both direction, and determinand is easy to produce shade and polishing is inhomogeneous, allows lighting effect often not as expected; In addition, when determinand is such as when the live body such as zebra fish or maggot, if excitation source is crossed strong or prolonged exposure, will make observing environment temperature rise too high, and easily cause live body Yin Gaowen and accelerate death; In addition, if will gather fingerprint for example on glass time, due to the existence of snake belly tube, cannot, by a simple light shield, easily outside veiling glare be covered, also can make the quality badness of institute's pick-up image, even failed.
As shown in Figure 2, applicant applies for disclosing in Chinese 201020110218.X utility application case " microscopy device used for fluorescence detection ", for solving the problems such as above-mentioned non-uniform light and excess Temperature, to adopt many light-emittingdiodes 91 ', and arrange luminously with annular, obtain fluoroscopic image for camera 90 ' or other image capture units; In addition, in No. 200910143131.4 application for a patent for invention case of the China having applicant " ring-shaped light source device of variable projection angle ", also propose to change lighting angle, use the floor light of details in a play not acted out on stage, but told through dialogues image light source is provided.
But, no matter how light source luminescent angle changes, that can only get rid of at most direct reflection disturbs light beam the most strongly, because the surface of general object is not real definitely smooth, therefore in the time being subject to illumination, unavoidably can be towards scatter reflections from all directions, this kind of irreflexive phenomenon, is not but to change above-mentioned light source the problem that angle just can solve is easily set.But compared to faint fluorescence or phosphorescence, diffuse even this and also can form the interference of certain degree.
Further, in field of medical examination, for reducing the actual bodily harm for sufferer, endoscopy surgery is widely used gradually at present, and so-called endoscopy surgery is much all the outer tube thicker by, stretch into the affected part of sufferer, in the caliber of deflection, be provided with an optical fiber, outside light source is imported and irradiation affected part; Again by affected part image transmission to outside screen, for healthcare givers observe.Stretch in the outer tube of introscope of affected part, be more provided with accurate apparatus, the ultrasound scanner head that for example calcination wound closure is used, or the mechanical chuck of acquisition portion of tissue, allow healthcare givers immediately test or treatment is operated on by Wicresoft.
Especially for example cancer, because cancer mutant has the function of bringing out angiogenesis, use the nutrient of grabbing normal cell tissue, allow cancer cell grow by paraplasm, therefore often can be at patient's affected area, the blood vessel noting abnormalities gathers, this is also to differentiate the common mechanism of one that cancer focus exists, in the processing of this type of disease, healthcare givers can be mixed with fluorescence by injection, the normal saline solution intravasation of phosphorescence or radioactive dyes, look for the region of confirming photoluminescence or radioactivity high response, thereby judge lesions position and regional extent.For example hematoporphyrin derivant (Haemato porphyrin Derivative, HpD) can send red fluorescence under light source irradiates.
But unfortunately, for avoiding the injury for patient, introscope must be in minimum pipe, institute can the required all constructional devices of ground installation extremely to the greatest extent, therefore cannot provide in the situation of the above-mentioned criminal identification of picture or research live body, allow multiple light-emittingdiodes directly around affected part, by luminous irradiation from all directions; Namely, light source is limited to be imported by outside, and be often that vertical irradiation is at lesions position, relief focus image is with almost identical path reflection passback, this structural limitations, can make the light disturbance of directly reflection cannot avoid completely, real important photoluminescence image, intensity often, not as good as one of direct catoptrical percentage, is greatly affected the accuracy of medical judgment.
Further, for filtering direct reflection and diffusing, the filter of specific wavelength must be set in light path, the loss while making to filter often reaches more than 40%, also allows the image data of actual detection is more difficult to be obtained by clear.Especially when some testing process, the fluorescence or the phosphorescence that have two or more disengage, for example, by blue-light excited green light, and green light activated red light.On last, green light represents the image data that will capture, and rear one on, the green light reflecting is the veiling glare of wanted filtering, and prior art cannot be differentiated above-mentioned two kinds of green light completely, therefore must adopt two kinds of distinct optical devices of wavelength, successively repeat above-mentioned detection for twice, once for green light image data, allow that green light passes through; Another time, for red light image data, stops green light to be passed through as far as possible.Such detection not only bothers, and also may allow image data overlappingly present, and causes the puzzlement of judgement.
Be worth mentioning, no matter directly reflected light or diffuse, is all after photon is sent by light source, exposes to determinand surface and turns back with the light velocity, can be considered to be luminous " simultaneously " and just return.Relatively, because fluorescence or phosphorus are the photons that absorbs exciting light with the peripheral electron in each molecule, numerous excited state electronics is in a period of time subsequently, base state is originally got back in transition randomly, and discharge fluorescence or phosphorescence simultaneously, make time that fluorescence or phosphorescence are progressively released can be longer, after exciting light reflection, almost turn back simultaneously, have the gap of a time, also therefore extend the Observable time of photoluminescence data, therefore how to utilize this mistiming characteristic, solve direct reflected light, diffuse or external light source interference, promote the quality of photoluminescence image, be the problem that the present invention pays close attention to.
[summary of the invention]
An object of the present invention is to provide one to utilize the mistiming, effectively the direct reflected light of blocking-up returns with diffusing, the time difference type fluoroscopic examination light supply apparatus of avoiding image to disturb, uses and significantly promotes the quality of photoluminescence image and the success ratio of image capture.
Another object of the present invention is to provide the one general optical image capture device of can arranging in pairs or groups, make it have the time difference type fluoroscopic examination light supply apparatus of fluorescence or phosphorescence acquisition ability, effectively promote use elasticity.
The time difference type fluoroscopic image acquisition system that provides a kind of operational use time poor is provided a further object of the present invention, effectively blocks direct reflected light and diffuses and return, and uses and significantly promotes the quality of photoluminescence image and the success ratio of image capture.
Another object of the present invention is to provide a kind of time difference type fluoroscopic image acquisition system with shade, further covers extraneous background miscellaneous light, promotes the quality of fluoroscopic image.
The time difference type fluoroscopic image acquisition system that provides a kind of operational use time poor is provided a more object of the present invention, allowing medical fluorescence or phosphorescence judge can be more accurate, effectively promote Medical Quality, reduce unnecessary erroneous judgement and delay, guarantee that sufferer is subject to appropriate treatment.
The time difference type fluoroscopic image acquisition method that provides a kind of operational use time poor is provided another object again of the present invention, not only raising obtains the success ratio of photoluminescence image, also can promote image quality simultaneously, allow the information being present in photoluminescence image effectively be obtained.
Of the present invention again again an object be to provide one can allow exciting light irradiate with stop irradiating repeatedly staggered, allow fluorescence or phosphorescence information constantly superpose and the time difference type photoluminescence image acquisition method of enhancing photoluminescence image brilliance, more further promote the success ratio of acquisition photoluminescence image.
For reaching above-mentioned purpose, the present invention discloses a kind of time difference type fluoroscopic examination light supply apparatus, for coordinating one group of shutter, to capture a photoluminescence image that is arranged at the determinand of a predetermined position to be measured, this time difference type fluoroscopic examination comprises with light supply apparatus: one group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured; And one group luminous and feedback signal and connect the controller of above-mentioned shutter in order to the above-mentioned pulse excitation light source assembly of instruction.
Time difference type fluoroscopic examination is installed in to image acquisition system with light supply apparatus, can obtain the image acquisition system that the present invention discloses, comprise: one group of time difference type fluoroscopic examination light supply apparatus, comprises one group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured; And one group in order to the luminous controller of the above-mentioned pulse excitation light source assembly of instruction; One group receives the shutter device that above-mentioned controller feedbacks signal, cuts out and open at the rear scheduled delay of luminous end in the time that above-mentioned pulse excitation light source assembly is luminous; And one group of image capture unit that is formed with an image data entrance, this image data entrance is corresponding to above-mentioned shutter device.
In addition, the present invention more provides a kind of method that captures photoluminescence image, be the photoluminescence image that is provided with one of photoluminescence image acquisition system acquisition and is arranged at the determinand of a predetermined position to be measured, aforementioned photoluminescence image acquisition system comprises one group of time difference type light supply apparatus for fluoroscopic examination, one group of shutter device and a group image capture device; Wherein above-mentioned time difference type fluoroscopic examination comprises one group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured with light supply apparatus; And one group of signal connects the controller of above-mentioned pulse excitation light source assembly; Above-mentioned image capture unit is formed with an image data entrance corresponding to above-mentioned shutter device, the method comprises the following steps: a) to close above-mentioned shutter device, and luminous to above-mentioned predetermined position to be measured with pulse mode by the above-mentioned pulse excitation light source assembly of above-mentioned controller instruction; And a b) scheduled delay after above-mentioned pulsed illumination finishes, the above-mentioned shutter device of instruction is opened, and allows above-mentioned image capture unit via above-mentioned image data entrance pick-up image data.
The present invention utilizes the mistiming between fluorescence or phosphorescence and reflected light dexterously, light source by pulsed irradiates, and shutter release is controlled on synchronization ground, the interference of blocking direct reflected light and diffusing, only allow the photoluminescence Zixuntong mistake after reflected light finishes, make the method for disclosed time difference type light supply apparatus, image acquisition system and acquisition photoluminescence image for fluoroscopic examination, can promote the success ratio of acquisition photoluminescence image, also effectively reduce light disturbance and promote the quality of fluoroscopic image; More can assist a ruler in governing a country by shade, effectively filter extraneous veiling glare; In addition, light supply apparatus also can be connected easily in optical equipments such as known camera, microscope, camera cell phones or merely with naked-eye observation, not only increase its range of application, and do not have the problem of sacrificing fluorescence or phosphorescence quality, meet various application requirements, thereby reach above-mentioned all objects.
[accompanying drawing explanation]
Fig. 1 is the schematic perspective view of a kind of existing camera and secondary light source;
Fig. 2 is the schematic perspective view of another kind of existing camera and floor light light source, illustrates that annular arranges luminous, and the floor light light source of details in a play not acted out on stage, but told through dialogues image can be provided;
Fig. 3 is the image acquisition system block schematic diagram of the present invention's the first preferred embodiment, and light supply apparatus of the present invention and camera collocation interactive relationship are described;
Fig. 4 is the operational flowchart of Fig. 3 embodiment;
Fig. 5 is the present invention's the second preferred embodiment, illustrates how light supply apparatus of the present invention coordinates existing introscope operation;
Fig. 6 is the light supply apparatus mechanical shutter structural representation of Fig. 5 embodiment;
Fig. 7 is the present invention's the 3rd preferred embodiment schematic perspective view;
Fig. 8 is the present invention's the 4th preferred embodiment schematic side view, illustrates how image acquisition system of the present invention coordinates existing microscope running.
[main element symbol description]
1 ... light supply apparatus 8 ... S.L.R
9 ... secondary light source 10 ... power supply module
11 ' ... motor 12,12 ", 12 " ' ... pulse excitation light source assembly
13 ' ... halo 13 " ... liquid crystal module
13 " ' ... shutter 14 ... controller
15 " ... start button 16 ... communication part
18 ' ... housing 50,51,52,53,54 ... step
80 ... shutter release button 82 ... shutter
86 ... transmission assembly 90 ' ... camera
91 ... snake belly tube 91 ' ... light-emittingdiode
130 ' ... slit 180 ' ... flexible hollow shell
182 ' ... optical fiber 70 ... microscope
72 ... pedestal
[embodiment]
About aforementioned and other technology contents, feature and effect of the present invention, in the detailed description of the following preferred embodiment that coordinates Figure of description, can clearly present; Same or analogous element, indicates the label with similar.
As shown in Figure 3, time difference type fluoroscopic examination in this example is to use with S.L.R 8 collocation with light supply apparatus 1, and be installed in the camera lens front of S.L.R 8, owing to there being at present S.L.R to be provided with USB Port and bluetooth communication device, as long as suitable program is write to this camera in advance, the excitation source can allow this routine light supply apparatus 1 take as fluoroscopic image time.In this example, time difference type fluoroscopic examination mainly comprises with light supply apparatus 1: power supply module 10, pulse excitation light source assembly 12, controller 14 and be illustrated as the communication part 16 of bluetooth device.
For the purpose of explanation, in this example, be searching warrant as example using criminal identification, and the outward appearance of this routine light supply apparatus 1 is roughly similar to earlier figures 2,8 of S.L.Rs comprise that shutter release button 80, shutter 82 and one group illustrate the transmission assembly 86 of corresponding bluetooth device.Main difference exists, and the camera shutter of known technology can be opened in the time of light source luminescent, also therefore allows photo-sensitive cell be subject to the direct reflection of determinand and the interference that diffuses; Relatively, S.L.R 8 of the present invention, Once you begin carry out above-mentioned formula, as shown in Figure 4, when operator is according to be accustomed in step 50 in the past, the shutter release button 80 of pressing S.L.R 8 tops, camera shutter 82 can't be opened immediately, but through transmission assembly 86, instruction signal is passed to the communication part 16 of light supply apparatus 1; And the pulse excitation light source assembly 12 that is illustrated as ultraviolet leds in step 51 by controller 14 instructions sends after the set of pulses light that for example continues 20 milliseconds (ms), stop immediately luminously in step 52, and feedback signal to the transmission assembly 86 of S.L.R 8.
When receiving transmission assembly 86, magazine processor (not shown) transmits signal, confirm that pulse excitation light source assembly 12 has stopped luminous, namely directly after reflected light and synchronous disappearance that diffuse almost, just really allow shutter 82 open for example 30ms in step 53, close after capturing smoothly faint photoluminescence data; Because photoluminescence data is faint, such time may be not enough to provide abundant exposure, therefore in step 54, determined again to carry out by the processor of camera stop after the pulsed illumination of above-mentioned 20ms luminous, and the loop of exposure 30mn, repeatedly above-mentioned steps 51 to 53 until fully exposure, obtain smoothly image and just finish loop.
Due to the general light beam in order to fluorescence excitation or phosphorescence, while exposing to fluorescence or phosphorescence thing to be observed, incident beam is directly reflection and returning mostly, or scattered by diffuse reflection, and be really few by the part of fluorescence or phosphorus absorption, make the real fluorescence producing compared to aforementioned reflection and the amount diffusing, very little in fact, even if change the lighting angle of light source, avoid the direct reflected light of maximum to disturb, if but irreflexive light beam and fluorescence, phosphorescence enters camera lens or human eye simultaneously, will severe jamming wish fluorescence or the phosphorescence image quality of observation.
In view of sustainable several seconds of phosphorescence, whether have containing a biological corpse or other object for laboratory examination and chemical testing (as sweat spot when for example inspecting exhibit, seminal stain, salivary stain etc.) time, the phosphorescence reagent of can arranging in pairs or groups, and time difference type fluoroscopic examination is closed with the first instruction shutter 82 of the controller 14 in light supply apparatus 1, and command pulse excitation source assembly 12 sends for example ultraviolet light to predetermined position to be measured, until this end-of-pulsing, the reflected light of turning back back all utilizes mistiming mode to be blocked with diffusing, thereby filter out the remainder light beam directly reflecting from target area, controller 14 is feedback rapidly signal again and is opened with order shutter 82, at this moment, can capture easily the phosphorescence image of determinand.
That is to say, utilize controller 14 to carry out the switch of gating pulse excitation source assembly 12 and shutter 82, allow reflected light and photoluminescence information distinguish to some extent, obtain suitable phosphorescence image data; Certainly, if will take normal white light image in contrast time, operator can take off time difference type fluoroscopic examination light supply apparatus by footpath row, and the photography that recovers rapidly Natural light environment is able to mutually compare with previous photoluminescence image; In addition, due to photoluminescence intensity a little less than, so need the exposure of long period, the shooting effect that just can obtain.
Via above-mentioned flow process, originally may form the direct reflected light of interference and diffuse in its crested reflection interval, originally faint photoluminescence image can make via multiexposure, multiple exposure the stack of image data intensity, even can be by the shade that is arranged on forefront, avoid outside veiling glare to enter interference, allow image quality promote, more can correctly obtain the message that originally will seek.Further, due to the enhancement of cell-phone function, be equipped with the camera cell phone of image acquisition system to become already the market mainstream, light supply apparatus of the present invention also can be selected the camera cell phone of arranging in pairs or groups, picture photographing effect of the photoluminescence such as fluorescence or phosphorescence is provided, significantly promotes application elasticity of the present invention.
Certainly; as be familiar with the art personage and can understand easily; above-mentioned shutter may not separate with light supply apparatus setting; also be not limited to the form of for example camera shutter; in the present invention's the second preferred embodiment as shown in Figure 5; take the introscope that is applied in medical domain as example; time difference type fluoroscopic examination comprises housing 18 ' with light supply apparatus and is installed in the shutter of housing 18 '; wherein; because housing 18 ' is extended with a bent flexible hollow shell 180 '; use protection, cover with restriction and bury optical fiber 182 ' wherein underground, claim that this optical fiber is optical path herein.Incident light and the light of being passed back all must be via optical fiber 182 ' the guiding transmission in flexible hollow shell 180 ', and be provided with a mechanical shutter as shown in Figure 6 at introscope operating portion, drive the 13 ' rotation of a slice halo via the motor 11 ' that for example rotating speed 5,000 per second turns, on halo 13 ', portray for example multiple open slots 130 ' of four, between each slit 130 ' and a time slit 130 ', can form the time interval of 50ns, when aperture opening ratio is 20%, represent that every the penetrating time of 10ns light the light time to chopping of 40ns will be had.
Through planning, in the 10ns that pulse excitation light source assembly can pass through at slit, allow incident light enter optical fiber 182 ' via one of them slit 130 ', for ease of explanation, defining this slit is entrance slit, and skip over optical fiber light inlet at slit, after illumination is blocked by the wall of halo 13 ', obtain by another slit 130 ' synchronization ground, position the fluorescence returning through optical fiber 182 ', the slit returning at this definition fluorescence is capture slit, owing to generally can reaching 10-5 second the perdurability of fluorescence, namely can after stopping, illumination continue about 10ns, therefore just can allow the synchronization of fluorescence information and return to via above-mentioned capture slit the image capture unit that is illustrated as CCD, and pass through equally multiple smooth incident and capture cycle, by the fluoroscopic image data stack obtaining, fluoroscopic image data clearly for example can be shown in general LCD screen, for check or healthcare givers with reference to.
That is pulse excitation light source assembly of the present invention may not be confined to itself need be luminous with impulse form, this routine pulse excitation light source assembly is exactly the entrance slit based on halo, makes its incident light that is incident to optical fiber present impulse form.When actual use, can first the material that is rich in the multiple fluorescence such as such as red, green, blue be injected in patient body, optionally generation effect be stranded in pathological tissues of fluorescent material, send for example white light by pulse excitation light source assembly subsequently, the blocking-up optical path transmission by halo rotation again; pathological tissues position can produce multi-fluorescence simultaneously; and return through the conduction of optical fiber; image capture unit obtains all various fluorescence data smoothly through the capture slit as shutter, and examiner just can see through the computer diagnostic pathology situation that naked eyes or outside connect.Especially further, because what import in this example is white light, only need to change motor rotary speed, can select to observe obtaining normal optical image.
And in practical application, because the retentivity time of eye of human eye is to be about the grade of 1/15 second, even directly obtain image with eyepiece observation introscope, also can be too short because of the above-mentioned time interval, the fluorescence data that observer is still thought return is a faint continuous luminous signal, can not know and perceive flicker, can not cause puzzlement depending on observation to naked, that does not also just observe is uncomfortable; In addition, this routine pulse excitation light source assembly also can be used in response to time difference type fluoroscopic examination the space size of light supply apparatus, and has the configuration mode of multi-form or quantity.
Again as shown in Figure 7, for example, will carry out authenticity of banknotes evaluation time, also image acquisition system of the present invention can be manufactured to the form as existing magnifier, using plural ultra-violet light-emitting diode as pulse excitation light source assembly 12 ", be arranged at shutter surrounding with loop configurations, and using a liquid crystal module 13 " as electronic shutter, when user's started by press button 15 " time, example is interpreted as pulse excitation light source assembly 12 " ultra-violet light-emitting diode will send ultraviolet light, and continue for example 20ns and expose to banknote to be identified, liquid crystal module 13 " see through and do not apply electric field, make disorderly arrangement of liquid crystal molecule and blocking-up light enters, and after UV-irradiation finishes, to liquid crystal module 13 " apply electric field, make liquid crystal molecule proper alignment and allow the follow-up photoluminescence such as fluorescence or phosphorescence image pass through.Similarly, due to the switching frequency of electronic shutter and light-emittingdiode, can observe speed far above human eye, therefore switch and will can not affect the observed image of operator rapidly.
Again as shown in Figure 8; many biological laboratories can be equipped with microscope 70 conventionally; and be removably placed on a pedestal 72, but due to the fluorescent microscope price general microscope close far above performance, therefore common laboratory not necessarily has fluorescent microscope.Once because experiment need to be observed such as fluorescin etc., just must buy more in addition and sell fluorescent microscope, this kind of resource consumption also do not meet general expectation, therefore, the fourth embodiment of the present invention is with one group, time difference type fluoroscopic examination light supply apparatus to be installed in one group of accessory (not label), and collocation is for example arranged at camera on microscope or video camera and jointly forms one group of photoluminescence image acquisition system.
Wherein, pulse excitation light source assembly 12 " ' be the shade inner side being arranged in this group accessory; one side stops outside veiling glare by shade and bias light enters object lens; another aspect is by shutter 13 " ' be arranged between shade and object lens, use and cause the above-mentioned mistiming, use the direct reflection of blocking-up in shade and diffuse, and allow that fluorescence Zixuntong crosses shutter 13 " ' and enter object lens for observing or record.Certainly, if possible produce the interference of wavelength, in this example, also can consider at shutter 13 " ' locate, or appropriate location in other optical paths installs filter additional, and further the noise of filtering close-spaced wavelength disturbs, and fluorescence microscopy image is met and detect the height requirement of analyzing.
Therefore, the present invention is by the design of time difference type, effectively cover extraneous veiling glare, reflection high light or diffuse, and allow that the photoluminescence images such as needed fluorescence or phosphorescence pass through, completely compatible with method of operating in the past on the one hand, also can more easily capture the image information of photoluminescence, and promote the quality of image data simultaneously; In addition, the present invention also can be connected easily in optical equipments such as known camera, microscope, camera cell phones or merely with naked-eye observation, it is widely used, and the system that the general nature photo-beat of also can arranging in pairs or groups is taken the photograph or directly observation, allow use elasticity to obtain significantly to promote and produce significant progress.
Only as described above, it is only preferred embodiment of the present invention, can not limit the scope of the present invention with this, every simple equivalence of doing according to the present patent application claims and description changes and modifies, and all should still remain within the scope of the patent.
Claims (12)
1. a time difference type fluoroscopic examination light supply apparatus, is for coordinating one group of shutter, and to capture a photoluminescence image that is arranged at the determinand of a predetermined position to be measured, this time difference type fluoroscopic examination comprises with light supply apparatus:
One group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured; And
One group luminous and feedback signal and connect the controller of above-mentioned shutter in order to the above-mentioned pulse excitation light source assembly of instruction.
2. time difference type fluoroscopic examination light supply apparatus as claimed in claim 1, more comprise that one driven by above-mentioned controller, just allow that at the rear scheduled delay of the luminous cut-off of above-mentioned pulse excitation light source assembly light penetrates, for the shutter of the above-mentioned determinand photoluminescence image of observation acquisition.
3. time difference type fluoroscopic examination light supply apparatus as claimed in claim 2, more comprises a housing that is formed with an optical path, and above-mentioned shutter is to be installed on this housing, for interdict this optical path in the time not driven by above-mentioned controller.
4. time difference type fluoroscopic examination light supply apparatus as claimed in claim 3, wherein this housing comprises a flexible hollow shell, and this optical path is the optical fiber being embedded in this flexible hollow shell.
5. the time difference type fluoroscopic examination light supply apparatus as described in claim 1,2 or 3, more comprises a shade that exposes to above-mentioned predetermined position to be measured for covering outer light.
6. time difference type fluoroscopic examination light supply apparatus as claimed in claim 5, more comprises a filter.
7. having an image acquisition system for time difference type fluoroscopic examination light supply apparatus, is the photoluminescence image that is arranged at the determinand of a predetermined position to be measured for one of acquisition, comprising:
One group of time difference type fluoroscopic examination light supply apparatus, comprises one group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured; And one group in order to the luminous controller of the above-mentioned pulse excitation light source assembly of instruction;
One group receives the shutter device that above-mentioned controller feedbacks signal, cuts out and open at the rear scheduled delay of luminous end in the time that above-mentioned pulse excitation light source assembly is luminous; And
One group is formed with the image capture unit of an image data entrance, and this image data entrance is corresponding to above-mentioned shutter device.
8. image acquisition system as claimed in claim 7, wherein above-mentioned shutter device is an electronic shutter.
9. image acquisition system as claimed in claim 8, wherein above-mentioned electronic shutter is a liquid crystal module.
10. image acquisition system as claimed in claim 7, wherein above-mentioned shutter device comprises a motor and a halo being rotated by aforementioned motor driving, is formed with the slit of at least one corresponding aforementioned image data entrance on this halo.
11. 1 kinds capture the method for photoluminescence image, be the photoluminescence image that is provided with one of photoluminescence image acquisition system acquisition and is arranged at the determinand of a predetermined position to be measured, aforementioned photoluminescence image acquisition system comprises one group of time difference type light supply apparatus for fluoroscopic examination, one group of shutter device and a group image capture device; Wherein above-mentioned time difference type fluoroscopic examination comprises one group of luminous pulse excitation light source assembly to above-mentioned predetermined position to be measured with light supply apparatus; And one group of signal connects the controller of above-mentioned pulse excitation light source assembly; Above-mentioned image capture unit is formed with an image data entrance corresponding to above-mentioned shutter device, and the method comprises the following steps:
A) close above-mentioned shutter device, and luminous to above-mentioned predetermined position to be measured with pulse mode by the above-mentioned pulse excitation light source assembly of above-mentioned controller instruction; And
B) scheduled delay after above-mentioned pulsed illumination finishes, the above-mentioned shutter device of instruction is opened, and allows above-mentioned image capture unit via above-mentioned image data entrance pick-up image data.
The method of 12. acquisition photoluminescence images as claimed in claim 11, more comprises repetition above-mentioned steps a) and b), until the step that reaches a predetermined exposure condition c).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107920191A (en) * | 2017-11-24 | 2018-04-17 | 广州腾德同普光电科技有限公司 | Material evidence trace obtains equipment |
| CN108187250A (en) * | 2018-02-07 | 2018-06-22 | 苏州元禾医疗器械有限公司 | A kind of automatic skin therapeutic device |
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| CN1457428A (en) * | 2001-02-28 | 2003-11-19 | 图像研究公司 | Imaging fluorometer for time resolved fluorescence |
| CN1804589A (en) * | 2005-12-28 | 2006-07-19 | 华南师范大学 | Method and apparatus for monitoring plant stress physiology and screening high-resistance plant |
| CN1898552A (en) * | 2003-12-19 | 2007-01-17 | 浜松光子学株式会社 | Harmful substance evaluating method and harmful substance evaluation kit |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1457428A (en) * | 2001-02-28 | 2003-11-19 | 图像研究公司 | Imaging fluorometer for time resolved fluorescence |
| CN1898552A (en) * | 2003-12-19 | 2007-01-17 | 浜松光子学株式会社 | Harmful substance evaluating method and harmful substance evaluation kit |
| CN1804589A (en) * | 2005-12-28 | 2006-07-19 | 华南师范大学 | Method and apparatus for monitoring plant stress physiology and screening high-resistance plant |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107920191A (en) * | 2017-11-24 | 2018-04-17 | 广州腾德同普光电科技有限公司 | Material evidence trace obtains equipment |
| CN107920191B (en) * | 2017-11-24 | 2019-08-23 | 广东腾德同普科技股份有限公司 | Material evidence trace obtains equipment |
| CN108187250A (en) * | 2018-02-07 | 2018-06-22 | 苏州元禾医疗器械有限公司 | A kind of automatic skin therapeutic device |
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Application publication date: 20140604 |