CN103834564A - Photic organism culture device - Google Patents

Photic organism culture device Download PDF

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Publication number
CN103834564A
CN103834564A CN201410076334.7A CN201410076334A CN103834564A CN 103834564 A CN103834564 A CN 103834564A CN 201410076334 A CN201410076334 A CN 201410076334A CN 103834564 A CN103834564 A CN 103834564A
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carrier
photo bio
cultivation apparatus
layer
nutritive medium
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CN103834564B (en
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王琳
朱振旗
陈传红
吴洪
罗少敬
陈昱
许�永
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ENN Science and Technology Development Co Ltd
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ENN Science and Technology Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14Scaffolds; Matrices

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Abstract

The invention relates to a photic organism culture device which comprises a supporting bracket and at least one carrier set suspended on the supporting bracket, wherein each carrier set comprises a plurality of carrier layers which are arranged at intervals; a carrier capable of adsorbing photic organism cells, a nutrient solution and a culture solution is arranged in each carrier layer; and for every two upper and lower adjacent carrier layers, the total area of the upper surface of all the carriers in the lower carrier layer is greater than the total area of the upper surface of all the carriers in the upper carrier layer. The photic organisms cultured by the photic organism culture device in such a structure have higher light receiving rate. Particularly, the carrier layers are arranged at intervals, and the total area of the upper surface of all the carrier in the lower carrier layer is greater than the total area of the upper surface of all the carriers in the upper carrier layer for every two upper and lower adjacent carrier layers, so the light receiving rate of the photic organism cells in the lower layer carrier can be enhanced, thereby enhancing the yield of the photic organisms.

Description

Photo bio cultivation apparatus
Technical field
The present invention relates to a kind of photo bio cultivation apparatus.
Background technology
Photosynthetic microorganism or vegetable cell, can utilize the form inorganicss such as sun power, water and simple mineral substance synthesis of organic substance and hydrogen, methane.Micro-algae is exactly one of them Typical Representative, and it is a class individual small photosynthetic autotrophs/type of supporting of holding concurrently, unicellular/simple multicellular organism, has the features such as widely distributed, of a great variety, photosynthetic efficiency is high, fast growth, strong adaptability.The annual fixing CO of micro-algae 2account for 40% of global net photosynthesis output, add it and be rich in the high value oxidation-resistance natural pigments such as lipid, hydro carbons, albumen, soluble polysaccharide, therefore micro-algae receives much attention in fields such as environmental protection, the energy and health cares.
Extensive, the high yielding culture of micro-algae are the bottlenecks of the micro-algae industrialized development of restriction, and the exploitation of low cost, high-efficient culture system and training method is the key that micro-algae large-scale is produced.Take " large water body is cultivated ", as main, its culture systems mainly comprises open raceway pond and sealing/semi-enclosed reactor to existing training method.Raceway pond mostly is oval or circular shallow pond, and in pond, mostly to be 20-30cm dark for algae liquid, by cylinder shape stirring rake, algae liquid is carried out to prolonged agitation, thereby realizes circulating of whole pond algae liquid, so that frustule and each nutrition distribution homogeneous in algae liquid.But the cultured output of raceway pond is always not ideal enough, mainly due to along with frustule growth, algae liquid turbidity raises gradually, daylight decay to zero after taking in several centimetres, algae liquid top layer, only top layer liquid level skim frustule visible ray carries out photosynthesis, and the mixed effect of algae liquid in the vertical direction is poor, the sensitive surface of unit volume algae liquid is less, and light utilising efficiency is low.Sealing/semi-enclosed reactor is pipe, post, tabular more, more than height can reach 2m, has the advantages such as unit occupied area glazing utilising efficiency is high, output is high.The light utilising efficiency of reactor increases with increase of its height, but meanwhile reactor middle part and bottom institute liquid body (algae liquid) pressure also increase, and cause reactor manufacturing cost and air feed energy consumption significantly to improve.In addition, reactor sensitive surface cannot be made corresponding adjustment with the variation of extraneous lamp, and height for reactor is subject to its manufacture material (particularly sensitive surface material) to possess the conditionality of better light transmission and higher-strength simultaneously, also makes the room for promotion of reactor light utilising efficiency be restricted.
U.S. Pat 20120107919A1 discloses a kind of multi-stage light reactor, and multiple-layer horizontal orifice plate is housed in this reactor, forms " cultivation region " of this reactor.Algae liquid pump is sprayed on first floor orifice plate to reactor top, the algae liquid falling within on first floor orifice plate flows down along hole on plate, and after being gathered into drop, the first floor orifice plate back side falls within on second layer orifice plate, on second layer orifice plate, algae liquid flows down and falls within after its back side is gathered into drop on the 3rd layer of orifice plate along hole on plate, so after drop falls into reactor bottom, is pumped to reactor top repetition said process again.CO 2pass into as nutritive element (carbon source) autoreactor bottom, make the frustule in drop be full of light source and CO 2growth in " cultivation region " of gas.In said structure, multiple-layer horizontal orifice plate is vertically arranged while being applied in physical environment, has serious Problem of Shading, causes frustule to be subject to light deficiency in this reactor " cultivation region " bottom.In addition, device can not carry out corresponding adjusting according to external light source variation and the different growth phase demands of frustule, to optimize spectrophotometric result.In addition carbon source (CO, 2/ containing CO 2mixed gas) pass into whole breeding spaces with gaseous form, mass-producing application difficulty is large, cost is high.
Summary of the invention
The object of the present invention is to provide a kind of photo bio cultivation apparatus of seeing that light rate is high.
For achieving the above object, provide a kind of photo bio cultivation apparatus, comprising: resting support; And hang at least one vehicle group on resting support, each vehicle group comprises the carrier layer that Spaced arranges, in each carrier layer, be provided with the carrier of adsorbable photo bio cell, nutritive medium and culturing liquid, wherein, in every adjacent upper and lower two carrier layer, in lower carrier layer, the total area of the upper surface of all carriers is greater than the total area of the upper surface of all carriers in carrier layer.
According to the present invention, in each vehicle group, the carrier layer of Spaced setting is most advanced and sophisticated taper upward.
According to the present invention, in each vehicle group, neighbouring carrier in two-layer is by proposing line series connection, and carrying between every upper and lower two-layer carrier is arranged with spacer tube on line.
According to the present invention, carrier is sheet shaped piece, and multiple through holes that run through sheet shaped piece upper and lower surface are set equably on sheet shaped piece; In every adjacent upper and lower two carrier layer, carry a through hole that penetrates the carrier that is positioned at upper strata after line is passed, merged by the multiple through holes of carrier that are arranged in lower floor.
According to the present invention, carrier is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, plastics, metal, synthetic plastics.
According to the present invention, carrier comprises the covering part of base portion and covering base portion, and covering part is made up of the sorbing material of adsorbable photo bio cell, nutritive medium and culturing liquid.
According to the present invention, carrier is concavo-convex chip architecture or the chip architecture that is provided with through hole.
According to the present invention, also comprise: for carrier provides the circulation device of nutritive medium or photo bio culturing liquid.
According to the present invention, circulation device comprises: be arranged at the cloth liquid part on resting support, the liquid outlet that cloth liquid part comprises fluid inlet and arranges towards carrier; And the infusion set being communicated with the fluid inlet fluid of cloth liquid part.
According to the present invention, resting support comprises: two compartment of terrains and the supporting bar being arranged in parallel; Two ends are connected in respectively the first strut member of two supporting bars movably, and wherein vehicle group hangs on the first strut member; Circulation device comprises: drive the first strut member to come and go mobile drive element along supporting bar.
Than prior art, beneficial effect of the present invention is:
1. on the carrier hanging on resting support, cultivate photo bio, can avoid in the time that Chi Tizhong cultivates, body bottom, pond photo bio is subject to the shortcoming of light deficiency, and the photo bio that makes to be positioned on carrier is fully subject to light, improve thus spectrophotometric result, thereby can improve the output of photo bio.In addition, the carrier layer arranging by Spaced, and in every adjacent upper and lower two carrier layer, in lower carrier layer, the total area of the upper surface of all carriers is greater than the total area of the upper surface of all carriers in carrier layer, that can improve lower floor's carrier glazing biomass cells sees light rate, improves thus the output of photo bio.Further, photo bio cultivation apparatus of the present invention is simple in structure, cost is low, and high compared with the high three-dimensional space availability ratio of expanding space because having, thus by this device for large-scale farming, improve thus the overall yield of photo bio.
2. by proposing the setting of line and spacer tube, can and loosen the space range of extension of carrying line adjusting carrier by tension, avoid to a certain extent or alleviated device self Problem of Shading, make bottom carrier see that light is more abundant.This structure can realize the light intensity that is subject to according to light intensity or photo bio self growth demand adjusting photo bio cell, thus for photo bio cell provides suitable illumination, to improve its productivity.
3. by the circulation device of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell) is provided for carrier, can be carrier supply nutritive medium or in supply nutritive medium, introduce new photo bio cell, in illumination abundance, guarantee that thus photo bio cell grows quickly.For example, by driving the first strut member to move downward, can or immerse fully carrier below by carrier part ground and be equipped with in the storage pool of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell), thus for carrier supply nutritive medium or supply nutritive medium time, introduce new photo bio cell.Again for example, by being arranged at the cloth liquid part on resting support, to carrier spraying nutrient solution or photo bio culturing liquid, thus for carrier supply nutritive medium or supply nutritive medium time, introduce new photo bio cell.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of first embodiment of photo bio cultivation apparatus of the present invention;
Fig. 2 is the vertical view of the photo bio cultivation apparatus shown in Fig. 1;
Fig. 3 is the mode of connection schematic diagram of the part carrier in the photo bio cultivation apparatus shown in Fig. 1;
Fig. 4 is the schematic diagram of second embodiment of photo bio cultivation apparatus of the present invention;
Fig. 5 is the schematic diagram of the 3rd embodiment of photo bio cultivation apparatus of the present invention;
Fig. 6 is the schematic diagram of the 4th embodiment of photo bio cultivation apparatus of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described.
See figures.1.and.2, first embodiment of photo bio cultivation apparatus of the present invention, comprises resting support 2; And hanging at least one vehicle group on resting support 2, each vehicle group comprises the carrier layer that Spaced arranges, and is provided with the carrier 4 of adsorbable photo bio cell, nutritive medium and culturing liquid in each carrier layer.Wherein, in every adjacent upper and lower two carrier layer, in lower carrier layer, the total area of the upper surface of all carriers is greater than the total area of the upper surface of all carriers in carrier layer, in other words, in as the vertical view of Fig. 2, the edge of lower carrier layer exceeds the edge of carrier layer, with improve lower carrier layer photo bio see light rate.Can understand, because vehicle group hangs on resting support 2, carrier 4 is subject to the direction of gravity identical with sensing from top to bottom.
Wherein, carrier 4 preferably, flexible high by water-retentivity, durable material is made, and it is slight to photo bio cell nontoxicity or toxicity.Alternatively, carrier 4 is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, plastics, metal, synthetic plastics, with absorption photo bio cell, nutritive medium and culturing liquid, certainly, be not limited to this.Or carrier 4 comprises the covering part of base portion and covering base portion alternatively, the base portion material with certain degree of hardness of serving as reasons is made, for example plastics, bamboo, and covering part is by adsorbable photo bio cell, the sorbing material of nutritive medium and culturing liquid is made, particularly, sorbing material is glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, plastics, metal, one or more materials in synthetic plastics, thus covering part is covered on base portion to form adsorbable photo bio cell, nutritive medium and culturing liquid, there is certain degree of hardness and in the time hanging, can keep the carrier 4 flattening.In the present embodiment, carrier 4 is by making on three layers of thick line cotton gauze bamboo circle that to stretch tight in outside diameter be 0.03m, and the base portion of carrier 4 is that outside diameter is the bamboo circle of 0.03m, and covering part is three layers of thick line cotton gauze.
On the carrier 4 hanging on resting support 2, cultivate photo bio, can avoid cultivating at Chi Tizhong, body bottom, pond photo bio is subject to the shortcoming of light deficiency, and the photo bio that makes to be positioned on carrier 4 is fully subject to light, improve thus spectrophotometric result, thereby can improve the output of photo bio.In addition, the carrier layer arranging by Spaced, and in every adjacent upper and lower two carrier layer, in lower carrier layer, the total area of the upper surface of all carriers is greater than the total area of the upper surface of all carriers in carrier layer, that can improve lower floor's carrier glazing biomass cells sees light rate, improves thus the output of photo bio.Further, photo bio cultivation apparatus of the present invention is simple in structure, cost is low, and high compared with the high three-dimensional space availability ratio of expanding space because having, thus by this device for large-scale farming, improve thus the overall yield of photo bio.
With further reference to Fig. 1 and Fig. 2, in each vehicle group, the carrier layer of Spaced setting is most advanced and sophisticated taper upward.Particularly, from top to bottom, first carrier layer arranges a carrier 4, hangs 3 carriers 4 and form second carrier layer on this carrier 4, each carrier 4 all directly hangs 3 carriers 4 afterwards, and directly hangs on the lower carrier layer of all carriers 4 compositions of carrier layer.Certainly, the quantity that hangs on a carrier 4 on carrier 4 is not limited to 3, according to actual cultivation needs, can change into other quantity.In this external every vehicle group, neighbouring carrier in two-layer is by proposing line series connection (with reference to Fig. 3), and carrying between every upper and lower two-layer carrier is arranged with spacer tube 5 on line.Carrier 4 is sheet shaped piece, and multiple through holes that run through sheet shaped piece upper and lower surface are set equably on sheet shaped piece; In every adjacent upper and lower two carrier layer, carry a through hole that penetrates the carrier that is positioned at upper strata after line is passed, merged by the multiple through holes of carrier that are arranged in lower floor.Preferably, in the present embodiment, 3 through holes that run through sheet shaped piece upper and lower surface are set on sheet shaped piece equably, and pass 3 by 3 through holes on a undermost carrier and carry line, carry line and jointly penetrate a through hole of a carrier 4 that is arranged in adjacent last layer for these 3, then carry line and each 3 through holes carrying line and jointly penetrate a carrier 4 that is arranged in adjacent last layer that passed by all the other two through holes of this carrier 4 for these 3, carry line and jointly penetrate for 9, by that analogy, until all carriers be together in series by carrying line.In the present embodiment, carrying line is the thick nylon wire of 0.005m, and spacer tube 5 is the lightweight transparent tube of outside diameter 0.01m, wall thickness 2mm.When light intensity is crossed strong or crosses weak to suppress or when restriction photo bio Growth of Cells, what can connect each carrier by adjusting puies forward line degree of tightness (strain and loosen), the relative angle of spacer tube between carrier is changed, and then the change carrier range of extension in space to the periphery, avoid to a certain extent or alleviated installing self Problem of Shading, thereby make photo bio cell obtain suitable light intensity, to improve its productivity.
In the present embodiment, resting support 2 comprises that two compartment of terrains and the supporting bar 7 being arranged in parallel and two ends are connected in respectively the first strut member 3 of two supporting bars 7 movably, and wherein vehicle group hangs on the first strut member 3.Can understand, the first strut member 3 is connected in two supporting bars 7, and the mode prop carrier 4 suspending in midair for carrier 4.Alternatively, the first strut member 3 can be rod member or the flexible strand of rigidity.In addition, in the present embodiment, be also included as carrier 4 the circulation device (not shown in figure 1) of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell) is provided, circulation device comprises that driving the first strut member 3 comes and goes mobile drive element and is positioned at the pond body 1 of carrier 4 belows along supporting bar 7.Alternatively, pond body 1 is brick square shallow pond, lined with PVC material, and the equal 5m of length and width, high 0.6m, hold nutritious liquid in pond body 1, and the dark 0.1-0.2m of nutritive medium.The first strut member 3 is made up of the rope made of hemp of diameter 0.05m, overall length 5m, and two ends are connected with diameter 0.035m Manila rope.On supporting bar, be provided with slide rail, and be provided with the pulley that can slide up and down with respect to slide rail, the above-mentioned rope made of hemp is connected in pulley, slides on slide rail through motor drive pulley.Thus, motor drive the first strut member 3 moves down and just can make carrier 4 immerse in nutritive medium to supplement the nutritive medium on carrier or make carrier 4 immerse in photo bio culturing liquid with supply nutritive medium and introduce new photo bio cell simultaneously, and motor drive the first strut member 3 moves up and just can make liquid level that carrier 4 departs from nutritive medium or culturing liquid to be all exposed under daylight.As above,, by the circulation device of nutritive medium or photo bio culturing liquid is provided for carrier 4, can in illumination abundance, guarantee that photo bio cell grows quickly.Certainly,, in embodiment alternatively, the first strut member 3 is preferably made up of water-fast, impregnating material, has good rigidity or snappiness.Alternatively, the first strut member 3 is by bamboo and wood, synthetic plastics, nylon, fiber crops or soak and mould one or more in metal and make.And supporting bar can be positioned at pond body 1 outside and is for example supported on ground, certainly, also can be positioned on the edge of pond body 1 inside or pond body 1.In addition, drive the first strut member 3 to come and go mobile drive unit and the first strut member 3 is not limited to this with the structure of supporting bar 7 along supporting bar 7, be alternately immersed in 1 neutralization of pond body and be exposed under daylight as long as can realize the vehicle group that makes to hang on the first strut member 3.
In the present embodiment, circulation device is to determine according to the absorption property of the concrete kind of photo bio and carrier 4 is common for carrier 4 provides nutritive medium or photo bio culturing liquid (containing nutritive medium and photo bio cell), in the present embodiment, in pond body 1, providing nutritive medium or photo bio culturing liquid is to determine according to the absorption property of the concrete kind of photo bio and carrier 4 is common.If what the photo bio of cultivating can be stable is adsorbed on carrier 4, be difficult to depart from and flow in pond body 1 by carrier 4 after the liquid immersing in pond body 1, in pond body 1, hold nutritive medium, can be regarded as and carry out solid culture.If and the photo bio of cultivating is easy to be departed from by carrier 4 and flow in pond body 1 (in the time that carrier 4 immerses in pond body 1 after the liquid immersing in pond body 1, the photo bio cell that has overwhelming majority absorption on carrier 4 flows in pond body 1, and photo bio cell in pond body 1 is understood some and is adsorbed onto on carrier 4), in pond body 1, hold photo bio culturing liquid, with in the time that each carrier 4 immerses, on carrier 4, supplement the photo bio culturing liquid (nutritive medium that contains photo bio cell is adsorbed onto on carrier 4) that inflow contains photo bio cell, maintain the photo bio cultivation quantity on carrier 4, can be regarded as liquid culture.Certainly, can understand, in the nutritive medium in the pond body 1 of above-mentioned solid culture, not contain photo bio cell completely, but in solid culture the content of the photo bio cell in nutritive medium far fewer than the content of the photo bio cell in culturing liquid in liquid culture.In the present embodiment, in pond body 1, hold nutritive medium, adopt solid culture mode.
Further narration below, by the process of above-mentioned photo bio cultivation apparatus cultivation photo bio, take cultivating microalgae as example.
In the present embodiment, carrier 4 is immersed and filled in the culture vessel of spirulina, and pass into air and CO 2mixed gas (wherein CO 2content is 0.5%-2%), under natural lighting condition, cultivate after 4 days, pull and form carrier 4 out vehicle group and hang on the first strut member 3.Establish altogether two resting support 2(not shown), 2 of two resting supports are at a distance of 2.5m.Within every 4 minutes, the first strut member 3 slowly moves to pond body 1 under the effect of electric motor, the carrier 4 hanging on the first strut member 3 is immersed in nutritive medium, after carrier 4 is completely submerged, motor drive the first strut member 3 with its on each carrier 4 rise from liquid level and be placed under daylight, and so forth.In addition, whole vehicle group and the first strut member can be around the axis perpendicular to the first strut member rotating connection, vehicle group can be rotated with the wind thus, also can improve to a certain extent light utilising efficiency.Within every 4-8 days, can gather in the crops the microalgae cell on carrier 4 one time, after results, remain in the conduct " seed " of microalgae cell on carrier 4, be directly used in a new round and cultivate.
In this embodiment, first residence time of strut member 3 in daylight adjusted, sets according to actual weather condition, when illumination is strong, shortened for first residence time of strut member 3 in daylight, and illumination increases by first residence time of strut member 3 in daylight when weak.Meanwhile, the residence time of the first strut member in daylight answered the liquid content of reference supports in addition.In addition, also can be provided with light intensity feedback system, the direction and intensity of light intensity feedback system measures illumination is also automatically controlled and is proposed the tension of line and loosen according to detected result, realizes automatically and regulating.
In breeding process, can directly in pond body 1, supplement the larger carbon source of micro algae growth consumption and (contain CO 2gas mixture, pure CO 2gas, flue gas, carbonate, supercarbonate etc.), phosphorus source (phosphoric acid salt), nitrogenous source etc., and the sterilizing agent polluting for prevention and control disease and pest, then by stirring or the mode such as aeration, the nutritive substance, the sterilizing agent etc. that fill into are uniformly distributed in pond.
Certainly, the structure of pond body is not limited to form shown in Fig. 1, can in pond body 1, dividing plate be set so that pond body 1 is divided into multiple sub-tanks, and nutrition/sterilizing agent can supplement respectively in sub-tank, also the nutritive medium in pond body 1 can be collected to rear unification and supplement.In addition, pond body 1 is for being positioned at the below of carrier 4, the device that the nutritive medium flowing down can be reclaimed.In pond body 1, also can fill the nutritive medium or the photo bio culturing liquid (including nutritive medium and microalgae cell) that are rich in micro algae growth desired nutritional material, use liquid culture.In addition, the method for attachment of the carrier between upper and lower two carrier layer is also not limited to said structure, and the material of carrying line and spacer tube is also not limited to above-described embodiment.For example, carry the preferred rope form of line, club shaped structure and by water-fast, anticorrosion and there is good rigidity or flexible material is made, alternatively by bamboo and wood, synthetic plastics, soak materials such as moulding metal, nylon, cotton, fiber crops and make.
Material, structure, size, making and the combined method of pond body, resting support, the first strut member, carrier in this embodiment; Mode of connection between carrier and the regulative mode of relative position; Circulation device; It is described that resting support spacing and the first support movements cycle etc. are all not limited to above-described embodiment.Can realize " dipping " nutritive medium; make microalgae cell carry out photosynthesis growth on implicit the carrier of nutritive substance; and the tapered setting of carrier; can be according to the variation of light intensity and direction of illumination; between adjusting carrier, relative position and carrier are at the range of extension in space, so that microalgae cell obtains any embodiment of more suitable growth conditions all in protection domain.
With reference to Fig. 4, second embodiment of this photo bio cultivation apparatus, wherein, exists together and repeats no more mutually with above-mentioned first embodiment.
In the present embodiment, carrier is the disk of being made up of 60 order nylon mesh and Zhu Quan, that is, the base portion of carrier 4 is that bamboo circle (annular element that bamboo is made), covering part are 60 order nylon mesh.Carrier is made rear direct formation vehicle group and is arranged on the first strut member 3 (without as first embodiment preculture 4 days in algae liquid), in pond body 1, be photo bio culturing liquid (being nutritive medium and microalgae cell in the present embodiment), algae kind is green chroococcoid, the first strut member 3 pumps once for every 2 minutes, when in pond body 1, microalgae density reaches 2-5g/L, in collecting tank, part microalgae cell to microalgae density in pond is 1-2g/L, starts a new round and cultivates.Can understand, in the photo bio cultivation apparatus of the present embodiment, in nutritive medium, microalgae cell can be adsorbed on carrier 4 together with nutritive medium, and can flow in pond body 1 with nutritive medium, form thus circulation, can think, in the time that carrier 4 proposes in by culturing liquid, can take a part of microalgae cell out of, this microalgae cell carries out photosynthesis growth under daylight, and in the time that carrier 4 immerses in culturing liquid again, part microalgae cell on carrier 4 flows into Chi Tizhong, and the new microalgae cell of the part of Chi Tizhong is attached to and leaves pond body 1 on carrier 4 and with carrier 4 and carry out photosynthesis, circulation thus, form liquid culture.
With reference to Fig. 5, the 3rd embodiment of photo bio cultivation apparatus of the present invention, wherein, repeats no more with first embodiment something in common.
In the present embodiment, the photo bio of cultivation is filamentous algae, and the first strut member 3 is made up of the thick bamboo pole of 0.07m and is directly fixed with supporting bar 7.Circulation device comprises the cloth liquid part 8 and the infusion set (not shown) that are arranged on resting support 2, the liquid outlet that cloth liquid part 8 comprises fluid inlet and arranges towards carrier 4, and the fluid inlet of cloth liquid part 8 is communicated with infusion set fluid.And infusion set is communicated with pond body 1, the nutritive medium in pond body 1 or photo bio culturing liquid can be pumped into the fluid inlet of cloth liquid part 8, in the present embodiment, in pond body 1, be nutritive medium, infusion set pumps into the nutritive medium in pond body 1 fluid inlet of cloth liquid part 8.With further reference to Fig. 5, cloth liquid part 8 is positioned on the first strut member 3, and in pond body 1, nutritive medium sprays in top layer carrier 4 by cloth liquid part via circulation device, i.e. carrier 4 in the carrier layer of the most close the first strut member 3.Nutritive medium is successively flowed through and is respectively attached with the carrier 4 of microalgae cell, supplies with moisture and the required multiple nutrients thing of growing for the microalgae cell on carrier 4, finally flows back in pond body 1.Wherein, in the present embodiment, cloth liquid part 8 is liquid distributor, and infusion set is water pump.
With reference to Fig. 6, the 4th embodiment of the present invention, wherein repeats no more with the 3rd embodiment something in common.In the present embodiment, carrier 4 is discoid, its covering part is that 50 order nylon mesh, base portion are bamboo circle, carrier 4 can be formed directly in vehicle group and is arranged on the first strut member 3 (without as first embodiment preculture 4 days in micro-algae liquid) after making, Chi Tizhong is photo bio culturing liquid (nutritive medium and microalgae cell), micro-algae kind is green chroococcoid, when in pond, microalgae density reaches 2-5g/L, in collecting tank, part microalgae cell to microalgae density in pond is 1-2g/L, starts a new round and cultivates.
Certainly, photo bio cultivation apparatus of the present invention is not limited to said structure.For example, preferably, carrier 4 is for concavo-convex chip architecture or be provided with the chip architecture of through hole, and concavo-convex chip architecture and the chip architecture that is provided with through hole all can improve the water retention capacity of carrier, in the time hanging carrier 4 can maintain wherein more water can be because deadweight departs from carrier 4.Thus, can reduce the load of circulation device, to reduce costs.In addition, when the mode to mention again after carrier is immersed to nutritive medium is during as carrier Additional nutrient solution, concavo-convex chip architecture and the chip architecture that is provided with through hole can be stayed more nutritive mediums in carrier 4 mentioning in process.
In addition, in the embodiment alternatively of photo bio cultivation apparatus of the present invention, also can comprise the travel mechanism that drives resting support to move, move better to make carrier follow the sun, realize more making full use of daylight.For example, in the time that pond body 1 is circular pond body, travel mechanism can drive resting support to rotate around the axis of circular pond body.
Certainly, whether the setting of pond body 1 can be basis according to whether needing to reclaim the nutritive medium that carrier 4 drips, and apply take the above-mentioned method that carrier 4 is dipped in pond body 1 to nutritive medium as basis.And this device can be built in original raceway pond, natural hole, pond, lakeside, make its fixing input low.And this device does not need to carry a large amount of water bodys (nutritive medium/photosynthetic microorganism nutrient solution), thereby water consumption, operation energy consumption and the equipment, the facility that have reduced breeding process drop into.In addition, in this device, carrier 4 can slowly, vertically pass in and out nutritive medium, resistance is little, and can be by gravitional force and other natural energy resources, therefore total energy consumption is low.
Alternatively, realize the mode that the first strut member moves along supporting bar and be not limited to above-described embodiment, for example, pulley is set on supporting bar, at the two ends of the first strut member stationary rope respectively, rope through pulley with utilize pulley that the first strut member is moved down or on carry.And drive such as electric motor of the optional use of drive element rope motion or that drive the first strut member to move along supporting bar, and propulsion source is preferably the eco-friendly power source such as sun power, tidal energy.In addition, circulation device also can be configured to be provided with the drive element of proposing line motion that driving is passed by the carrier in the superiors' carrier layer,, passed by the carrier in the superiors' carrier layer carry line and merge after alternatively through being fixed on pulley on support, drive element is pulled and is carried line by pulley, realize and propose moving down or moving up of line thus, thus vehicle group can be immersed in pond body 1 or on move the liquid level that departs from pond body 1.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims (10)

1. a photo bio cultivation apparatus, is characterized in that, comprising:
Resting support (2); And
Hang at least one vehicle group on described resting support (2), each vehicle group comprises the carrier layer that Spaced arranges, and is provided with the carrier of adsorbable photo bio cell, nutritive medium and culturing liquid in each carrier layer,
Wherein, in every adjacent upper and lower two carrier layer, in lower carrier layer, the total area of the upper surface of all carriers is greater than the total area of the upper surface of all carriers in carrier layer.
2. photo bio cultivation apparatus according to claim 1, is characterized in that,
In each vehicle group, the carrier layer of described Spaced setting is most advanced and sophisticated taper upward.
3. photo bio cultivation apparatus according to claim 1 and 2, is characterized in that,
In each vehicle group, neighbouring described carrier (4) in two-layer is by proposing line series connection, and carrying between every upper and lower two-layer carrier is arranged with spacer tube (5) on line.
4. photo bio cultivation apparatus according to claim 3, is characterized in that,
Described carrier (4) is sheet shaped piece, and multiple through holes that run through described sheet shaped piece upper and lower surface are set equably on described sheet shaped piece;
In every adjacent upper and lower two carrier layer, carry a through hole that penetrates the carrier that is positioned at upper strata after line is passed, merged by the described multiple through holes of carrier that are arranged in lower floor.
5. photo bio cultivation apparatus according to claim 1, is characterized in that,
Described carrier (4) is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, plastics, metal, synthetic plastics.
6. photo bio cultivation apparatus according to claim 1, is characterized in that,
Described carrier (4) comprises the covering part of base portion and the described base portion of covering, and described covering part is made up of the sorbing material of adsorbable photo bio cell, nutritive medium and culturing liquid.
7. photo bio cultivation apparatus according to claim 1, is characterized in that,
Described carrier (4) is concavo-convex chip architecture or the chip architecture that is provided with through hole.
8. photo bio cultivation apparatus according to claim 1, is characterized in that, also comprises:
For described carrier (4) provides the circulation device of nutritive medium or photo bio culturing liquid.
9. photo bio cultivation apparatus according to claim 8, is characterized in that, described circulation device comprises:
Be arranged at the cloth liquid part (8) on described resting support (2), the liquid outlet that described cloth liquid part (8) comprises fluid inlet and arranges towards described carrier (4); And
The infusion set being communicated with the fluid inlet fluid of described cloth liquid part (8).
10. photo bio cultivation apparatus according to claim 8, is characterized in that,
Described resting support (2) comprising: two compartment of terrains and the supporting bar (7) being arranged in parallel; Two ends are connected in respectively first strut member (3) of two described supporting bars (7) movably, and wherein said vehicle group hangs on described the first strut member (3);
Described circulation device comprises: drive described the first strut member (3) to come and go mobile drive element along described supporting bar (7).
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754239A (en) * 2016-11-16 2017-05-31 新奥科技发展有限公司 A kind of microalgae solid culture system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1062447A (en) * 1990-12-17 1992-07-08 林伟清 Layered tower planting trays
US20120107919A1 (en) * 2009-06-24 2012-05-03 Igv Institut Fuer Getreideverarbeitung Gmbh Method for Producing Biomass and Photobioreactor for Cultivating Phototrophic or Mixotrophic Organisms or Cells
CN103382443A (en) * 2012-05-02 2013-11-06 广西科学院 Novel method for culturing microalgae

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1062447A (en) * 1990-12-17 1992-07-08 林伟清 Layered tower planting trays
US20120107919A1 (en) * 2009-06-24 2012-05-03 Igv Institut Fuer Getreideverarbeitung Gmbh Method for Producing Biomass and Photobioreactor for Cultivating Phototrophic or Mixotrophic Organisms or Cells
CN103382443A (en) * 2012-05-02 2013-11-06 广西科学院 Novel method for culturing microalgae

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈必链: "《生物工程设备》", 30 September 2013 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106754239A (en) * 2016-11-16 2017-05-31 新奥科技发展有限公司 A kind of microalgae solid culture system
CN106754239B (en) * 2016-11-16 2019-02-26 新奥科技发展有限公司 A kind of microalgae solid culture system

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