CN103834475A

CN103834475A - Room-temperature gradient extraction of antioxidant from rosmarinus officinalis

Info

Publication number: CN103834475A
Application number: CN201410129306.7A
Authority: CN
Inventors: 姜立强
Original assignee: GUIZHOU REDSTAR DEVELOPMENT DUYUN LUYOU Co Ltd
Current assignee: GUIZHOU REDSTAR DEVELOPMENT DUYUN LUYOU Co Ltd
Priority date: 2014-04-02
Filing date: 2014-04-02
Publication date: 2014-06-04

Abstract

The invention relates to room-temperature gradient extraction of an antioxidant from rosmarinus officinalis. The gradient extraction is carried out by four steps under room temperature, and different rosmarinus officinalis antioxidants can be obtained at the same time. The room-temperature gradient four-level extraction process comprises the following steps of: crushing leaves of rosmarinus officinalis; distilling with water vapor; extracting essential oil; adding the residues and a non-polar solvent A to an extracting tank based on the ratio of 1: 6 to 1: 8; performing three-level extraction under proper temperature and pressure to obtain extracting liquid a and the filter residue; extracting the filter residue with a low-polar solvent B to obtain extracting liquid b and the filter residue; extracting the filter residue with a medium-polar solvent c to obtain extracting liquid c and the filter residue; extracting the filter reside with a strong-polar solvent D to obtain extracting liquid d and the filter residue, so as to obtain extracting liquid a, b, c, d and the rest filter residues from the four extractions; and then retting the filter residue to prepare organic fertilizer and biogas. With the adoption of the room-temperature gradient extraction, the loss of effective ingredients is decreased, the extracting rate of the effective ingredients is raised, the energy consumption is lowered down, and the variety and quality are improved.

Description

Room temperature gradient is extracted antioxidant from rosemary plant

Technical field

The present invention relates to a kind of method of extracting antioxidant, especially relate to and from rosemary plant, extract antioxidant by room temperature gradient.

Background technology

Antioxidant can stop particularly grease or the good antioxidant addn of oil-containing food of sanitas of food oxydative spoilage, it is Nantural non-toxic, at present conventional food preservatives is as formic acid, the Chemical Preservatives such as Sorbic Acid, oils antioxidant is TBH, these butylated hydroxy anisoles of the syntheticses such as BHT and butylated hydroxytoluene, to human liver, spleen, lungs etc. all have disadvantageous effect, even can cause lung cancer and hepatomegaly, wherein some product has been limited to use in some field of food, and rosemary antioxidant is the extract of natural phant, toxicological experiment proves its safety non-toxic, obtain the safety food raw material of U.S. FDA accreditation.High temperature resistant, radiation resistance good, ability 240

high temperature, can withstand long term exposure 190

high temperature is fried still has antioxidant effect, and has good solubility and stability.Stability Analysis of Structures is difficult for decomposing, and Rosmarinus officinalis is to have tea phenol structural framework---polycyclic polyphenolic compound, thereby its chemical property is very stable, is difficult for decomposing.Broad-spectrum high efficacy, Rosmarinus officinalis fat-soluble antioxidant is adding after various auxiliary shape agents, make a series of food that can be used for any system, lipoid oxidation to various complexity has extensively and very strong inhibition, under equal conditions, its resistance of oxidation is BHA, BHT, TBHQ 2-4 times, and the 1-2 of Ve, Vc doubly, is better than tea-polyphenol.Also there is efficient " carry and enter " ability.Good compatibility, it can be composite with the antioxidant compatibility such as tea-polyphenol, suppresses antibacterial ability strong, and rosemary antioxidant suppresses flavus, function bacterium, the moulds such as intestinal bacteria, compared with other oil antioxidants, there is safe and efficient cost low, be the antioxidant of green food first-selection, while or natural pigment stablizer, it is light green pressed powder, is convenient to transport, packaging, storage.Water-soluble rosemary antioxidant, except having fat-soluble above-mentioned feature, also there is structural constituent single, tawny powder and soluble in water, there is strong antiviral dirt and anti-inflammatory action, disturb the active of different approaches C3 convertase and can eliminate Bai Youji.

The production technique of China's rosemary extract is mainly two-step approach at present, first in Rosmarinus officinalis, extract essential oil, then extract effective constituent wherein with all kinds of SOLVENTS, the benefit of this technique is the peculiar smell of having removed extract, and the simple easy handling of technique, shortcoming is the product due to simultaneous extraction different size, causes the loss of effective ingredient serious, and extraction yield is not high.

The greenhouse gradient extraction method that the present invention works out, has effectively solved above problem, produces effect as a trial fine by industrialization.

Summary of the invention

The object of this invention is to provide a kind of room temperature gradient and from rosemary plant, extracted antioxidant, under greenhouse experiment, extract by four step steps, can obtain the rosemary antioxidant of different size simultaneously, room temperature gradient level Four extraction process: first Rosmarinus officinalis blade is pulverized, carry out steam distillation, extract essential oil, again residue and row's polar solvent A are added in extractor by 1:6-1:8, under suitable temperature, pressure, carry out three grades of extractions, be extracted a and filter residue, extract filter residue with weak polar solvent B again and be extracted liquid b and filter residue, with the solvent c extraction filter residue of middle polarity, be extracted liquid c and filter residue, finally extract filter residue with intensive polar solvent D, be extracted liquid d and filter residue, be extracted liquid a through 4 extractions, b, c, d and remaining filter residue, filter residue retting can be obtained to organic fertilizer and biogas.

Extraction liquid a is added in proportion to vehicle and can obtain the product of different size, by regulating concentration and the extraction time of solvent B, C, D, the processing condition such as temperature can corresponding adjusting extraction liquid a, the concentration of b, c, d, adopt air stream drying, vacuum-drying and obtain the fat-soluble antioxidant of different size except methods such as temperature.

Described non-polar solvent A is alkanes, and described weak polar solvent B is methyl alcohol or high concentration ethanol, and described middle grade solvent C is acetone, and the secret intensive polar solvent D stating is water, aqueous ethanolic solution.

Adopt the beneficial effect of the technical program: the antioxidant that simultaneously obtains at ambient temperature different size, reduce the loss of effective ingredient, improve the extraction yield of effective ingredient, reduce energy consumption, more original two-step approach has reduced cost, diversify, improved the quality of each product category.

Brief description of the drawings

Fig. 1 is the process flow sheet that room temperature room temperature gradient of the present invention is extracted antioxidant from rosemary plant.

Embodiment

Room temperature room temperature gradient of the present invention is extracted the processing method of antioxidant from rosemary plant, now contrasts process flow sheet and is further described:

1, the pulverizing of Rosmarinus officinalis blade is distilled with water vapour, obtain the essential oil and the residue that extract;

2, residue is used to non-polar solvent A, added to and in extractor, under suitable temperature, pressure, carry out three grades of extractions and be extracted liquid a and filter residue in the ratio of 1:6-1:8;

3, extraction liquid a is added to excipient, obtain product 1, product 2, the product 3 of rosemary antioxidant.

4,, by the filter residue of extraction liquid a, with weak polar solvent B extraction filter residue, be extracted liquid b and filter residue.

5, extraction liquid d is dried, obtains product 4;

6,, by the filter residue of extraction liquid b, with medium utmost point solvent c extraction filter residue, be extracted liquid c and filter residue;

7, extraction liquid c is dried, obtains product 5;

8, the filter residue of extraction liquid c is extracted to filter residue with intensive polar solvent D, be extracted liquid d and filter residue;

9, extraction liquid a is dried and obtains product 6;

10, the filter residue retting of extraction liquid a is become to organic fertilizer or the raw material as product biogas.

Claims

1. room temperature gradient is extracted an antioxidant from rosemary plant, it is characterized in that: the rosemary antioxidant that obtains at ambient temperature multiple different sizes by four step steps; The steps include: that (1) pulverize Rosmarinus officinalis blade to distill with water vapor, obtain the essential oil and the residue that extract; (2) by residue as polar solvent A, add to and in extractor, under suitable temperature, pressure, carry out three grades of extractions and be extracted liquid a and filter residue in the ratio of 1:6-1:8; (3) extraction liquid a is added to excipient, obtain product 1, product 2, the product 3 of rosemary antioxidant, by the filter residue of extraction liquid a, with weak polar solvent B extraction filter residue, be extracted liquid b and filter residue; (4) extraction liquid d is dried, obtains product 4.

2. from rosemary plant, extract antioxidant according to the room temperature gradient of claim 1, it is characterized in that: described non-polar solvent A is alkanes, described weak polar solvent B is methyl alcohol or high concentration ethanol, described middle grade solvent C is acetone, the secret intensive polar solvent D stating is water, aqueous ethanolic solution, by the filter residue of extraction liquid b, with medium utmost point solvent c extraction filter residue, be extracted liquid c and filter residue.

3. from rosemary plant, extract antioxidant according to the room temperature gradient of claim 1, it is characterized in that: extraction liquid c is dried, obtains product 5.

4. from rosemary plant, extract antioxidant according to the room temperature gradient of claim 1, it is characterized in that: the filter residue of extraction liquid c intensive polar solvent D is extracted to filter residue, be extracted liquid d and filter residue.

5. from rosemary plant, extract antioxidant according to the room temperature gradient of claim 1, it is characterized in that: extraction liquid a is dried and obtains product 6.

6. from rosemary plant, extract antioxidant according to the room temperature gradient of claim 1, it is characterized in that: the filter residue retting of extraction liquid a is become to organic fertilizer or the raw material as product biogas.